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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G18310 |
| fragaria_vesca | FvH4_5g25850 |
| malus_domestica | MD04G1026400.v1.1 |
| prunus_persica | Prupe.2G050600_v2.0.a1 |
| pyrus_communis | pycom04g02250 |
| rosa_chinensis | RchiOBHm_Chr6g0264721 RchiOBHm_Chr6g0264731 RchiOBHm_Chr7g0216331 |
| rosa_laevigata | RLG00000002581 |
| rosa_multiflora | Rmu_sc0015060.1_g000019 |
| rosa_roxburghii | Rroxscaffold_3G00243320 |
| rosa_rugosa | Rorug07G0160000 |
| rosa_samantha | Rh7AG297400 Rh7BG289700 Rh7CG317600 Rh7DG299800 |
| rosa_wichuraiana | Rw7G025300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 849, 2490 |
| Acc16I | TGCGCA | 1 cut(s) | 1091 |
| Acc36I | ACCTGC | 1 cut(s) | 2296 |
| AccBSI | CCGCTC | 1 cut(s) | 2721 |
| AccI | GTMKAC | 1 cut(s) | 2470 |
| AciI | CCGC | 5 cut(s) | 689, 1866, 2128, 2284, 2719 |
| AclWI | GGATC | 2 cut(s) | 1309, 1665 |
| AcuI | CTGAAG | 1 cut(s) | 1344 |
| AfaI | GTAC | 6 cut(s) | 278, 566, 672, 1715, 2070, 2184 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 21 |
| AgeI | ACCGGT | 1 cut(s) | 365 |
| AhlI | ACTAGT | 1 cut(s) | 1028 |
| AjnI | CCWGG | 3 cut(s) | 1346, 1380, 2158 |
| Alw21I | GWGCWC | 1 cut(s) | 1356 |
| Alw26I | GTCTC | 1 cut(s) | 317 |
| AlwI | GGATC | 2 cut(s) | 1309, 1665 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 221 |
| AoxI | GGCC | 3 cut(s) | 265, 1205, 2108 |
| ApaI | GGGCCC | 1 cut(s) | 269 |
| ApeKI | GCWGC | 5 cut(s) | 1456, 1645, 2130, 2286, 2444 |
| AsiGI | ACCGGT | 1 cut(s) | 365 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 321 |
| AspA2I | CCTAGG | 2 cut(s) | 1700, 2111 |
| AspLEI | GCGC | 3 cut(s) | 1092, 1435, 1461 |
| AspS9I | GGNCC | 9 cut(s) | 92, 265, 266, 527, 605, 795, 1277, 2177, 2570 |
| AsuHPI | GGTGA | 3 cut(s) | 204, 380, 2174 |
| AvaII | GGWCC | 7 cut(s) | 92, 527, 605, 795, 1277, 2177, 2570 |
| AvrII | CCTAGG | 2 cut(s) | 1700, 2111 |
| AxyI | CCTNAGG | 1 cut(s) | 1567 |
| BaeGI | GKGCMC | 1 cut(s) | 269 |
| BanII | GRGCYC | 3 cut(s) | 269, 1123, 1356 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 2175, 2207, 2699, 2731 |
| BbsI | GAAGAC | 3 cut(s) | 43, 643, 1500 |
| Bbv12I | GWGCWC | 1 cut(s) | 1356 |
| BbvI | GCAGC | 5 cut(s) | 1468, 1632, 2142, 2298, 2456 |
| BccI | CCATC | 1 cut(s) | 2375 |
| BceAI | ACGGC | 1 cut(s) | 2104 |
| BciT130I | CCWGG | 3 cut(s) | 1348, 1382, 2160 |
| BclI | TGATCA | 2 cut(s) | 2338, 2356 |
| BcoDI | GTCTC | 1 cut(s) | 317 |
| BcuI | ACTAGT | 1 cut(s) | 1028 |
| BfmI | CTRYAG | 1 cut(s) | 1735 |
| BfuAI | ACCTGC | 1 cut(s) | 2296 |
| BglII | AGATCT | 2 cut(s) | 1363, 2074 |
| BisI | GCNGC | 7 cut(s) | 1457, 1646, 2128, 2131, 2284, 2287, 2445 |
| BlnI | CCTAGG | 2 cut(s) | 1700, 2111 |
| BlpI | GCTNAGC | 1 cut(s) | 2279 |
| BlsI | GCNGC | 7 cut(s) | 1458, 1647, 2129, 2132, 2285, 2288, 2446 |
| Bme1390I | CCNGG | 3 cut(s) | 1348, 1382, 2160 |
| Bme18I | GGWCC | 7 cut(s) | 92, 527, 605, 795, 1277, 2177, 2570 |
| BmgT120I | GGNCC | 9 cut(s) | 92, 265, 266, 527, 605, 795, 1277, 2177, 2570 |
| BmiI | GGNNCC | 5 cut(s) | 93, 267, 796, 1800, 2178 |
| BmrFI | CCNGG | 3 cut(s) | 1348, 1382, 2160 |
| BmsI | GCATC | 8 cut(s) | 886, 898, 1092, 1137, 1420, 1607, 1762, 2605 |
| BpiI | GAAGAC | 3 cut(s) | 43, 643, 1500 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2279 |
| BpuEI | CTTGAG | 1 cut(s) | 1788 |
| BsaJI | CCNNGG | 4 cut(s) | 1347, 1700, 1977, 2111 |
| BsaWI | WCCGGW | 2 cut(s) | 365, 2179 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1269, 1299 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 21 |
| Bse118I | RCCGGY | 3 cut(s) | 365, 1105, 2437 |
| Bse21I | CCTNAGG | 1 cut(s) | 1567 |
| Bse3DI | GCAATG | 1 cut(s) | 1121 |
| BseBI | CCWGG | 3 cut(s) | 1348, 1382, 2160 |
| BseDI | CCNNGG | 4 cut(s) | 1347, 1700, 1977, 2111 |
| BseGI | GGATG | 4 cut(s) | 877, 1195, 1777, 2543 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 21 |
| BseMI | GCAATG | 1 cut(s) | 1121 |
| BseMII | CTCAG | 3 cut(s) | 1581, 2096, 2639 |
| BseRI | GAGGAG | 3 cut(s) | 199, 216, 1969 |
| BseSI | GKGCMC | 1 cut(s) | 269 |
| BseXI | GCAGC | 5 cut(s) | 1468, 1632, 2142, 2298, 2456 |
| BseYI | CCCAGC | 1 cut(s) | 1477 |
| BshFI | GGCC | 3 cut(s) | 267, 1207, 2110 |
| BshTI | ACCGGT | 1 cut(s) | 365 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1356 |
| BsiSI | CCGG | 4 cut(s) | 366, 1106, 2180, 2438 |
| BslFI | GGGAC | 3 cut(s) | 808, 830, 2607 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 21 |
| BsmAI | GTCTC | 1 cut(s) | 317 |
| BsmBI | CGTCTC | 1 cut(s) | 317 |
| BsmFI | GGGAC | 3 cut(s) | 808, 830, 2607 |
| BsmI | GAATGC | 2 cut(s) | 1391, 1868 |
| BsnI | GGCC | 3 cut(s) | 267, 1207, 2110 |
| Bsp120I | GGGCCC | 1 cut(s) | 265 |
| Bsp1286I | GDGCHC | 3 cut(s) | 269, 1123, 1356 |
| Bsp1407I | TGTACA | 1 cut(s) | 670 |
| Bsp143I | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2279 |
| Bsp19I | CCATGG | 1 cut(s) | 1977 |
| BspACI | CCGC | 5 cut(s) | 689, 1866, 2128, 2284, 2719 |
| BspANI | GGCC | 3 cut(s) | 267, 1207, 2110 |
| BspCNI | CTCAG | 3 cut(s) | 1580, 2095, 2640 |
| BspHI | TCATGA | 2 cut(s) | 1834, 2212 |
| BspLI | GGNNCC | 5 cut(s) | 93, 267, 796, 1800, 2178 |
| BspMI | ACCTGC | 1 cut(s) | 2296 |
| BspPI | GGATC | 2 cut(s) | 1309, 1665 |
| BsrBI | CCGCTC | 1 cut(s) | 2721 |
| BsrDI | GCAATG | 1 cut(s) | 1121 |
| BsrFI | RCCGGY | 3 cut(s) | 365, 1105, 2437 |
| BsrGI | TGTACA | 1 cut(s) | 670 |
| BssAI | RCCGGY | 3 cut(s) | 365, 1105, 2437 |
| BssECI | CCNNGG | 4 cut(s) | 1347, 1700, 1977, 2111 |
| BssMI | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| BssT1I | CCWWGG | 3 cut(s) | 1700, 1977, 2111 |
| Bst2UI | CCWGG | 3 cut(s) | 1348, 1382, 2160 |
| Bst4CI | ACNGT | 4 cut(s) | 311, 1067, 1164, 1374 |
| Bst6I | CTCTTC | 9 cut(s) | 38, 145, 234, 247, 696, 1410, 2196, 2382, 2663 |
| BstAUI | TGTACA | 1 cut(s) | 670 |
| BstC8I | GCNNGC | 7 cut(s) | 661, 1094, 1141, 1925, 1929, 1933, 2314 |
| BstDEI | CTNAG | 9 cut(s) | 919, 1328, 1440, 1567, 1794, 2082, 2279, 2648, 2680 |
| BstDSI | CCRYGG | 1 cut(s) | 1977 |
| BstF5I | GGATG | 4 cut(s) | 877, 1195, 1777, 2543 |
| BstHHI | GCGC | 3 cut(s) | 1092, 1435, 1461 |
| BstKTI | GATC | 8 cut(s) | 1304, 1366, 1660, 1886, 2077, 2341, 2359, 2590 |
| BstMAI | GTCTC | 1 cut(s) | 317 |
| BstMBI | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1102, 1111, 1386, 2116, 2372 |
| BstNI | CCWGG | 3 cut(s) | 1348, 1382, 2160 |
| BstNSI | RCATGY | 2 cut(s) | 677, 695 |
| BstSCI | CCNGG | 3 cut(s) | 1346, 1380, 2158 |
| BstSFI | CTRYAG | 1 cut(s) | 1735 |
| BstSLI | GKGCMC | 1 cut(s) | 269 |
| BstV1I | GCAGC | 5 cut(s) | 1468, 1632, 2142, 2298, 2456 |
| BstV2I | GAAGAC | 3 cut(s) | 43, 643, 1500 |
| BstX2I | RGATCY | 2 cut(s) | 1363, 2074 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2320 |
| BstYI | RGATCY | 2 cut(s) | 1363, 2074 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1567 |
| BsuRI | GGCC | 3 cut(s) | 267, 1207, 2110 |
| BtgI | CCRYGG | 1 cut(s) | 1977 |
| BtsCI | GGATG | 4 cut(s) | 877, 1195, 1777, 2543 |
| BtsIMutI | CAGTG | 1 cut(s) | 1547 |
| BveI | ACCTGC | 1 cut(s) | 2296 |
| Cac8I | GCNNGC | 7 cut(s) | 661, 1094, 1141, 1925, 1929, 1933, 2314 |
| CaiI | CAGNNNCTG | 1 cut(s) | 221 |
| CciI | TCATGA | 2 cut(s) | 1834, 2212 |
| CfoI | GCGC | 3 cut(s) | 1092, 1435, 1461 |
| Cfr10I | RCCGGY | 3 cut(s) | 365, 1105, 2437 |
| Cfr13I | GGNCC | 9 cut(s) | 92, 265, 266, 527, 605, 795, 1277, 2177, 2570 |
| CsiI | ACCWGGT | 1 cut(s) | 2158 |
| Csp6I | GTAC | 6 cut(s) | 277, 565, 671, 1714, 2069, 2183 |
| CspAI | ACCGGT | 1 cut(s) | 365 |
| CviQI | GTAC | 6 cut(s) | 277, 565, 671, 1714, 2069, 2183 |
| DdeI | CTNAG | 9 cut(s) | 919, 1328, 1440, 1567, 1794, 2082, 2279, 2648, 2680 |
| DpnI | GATC | 8 cut(s) | 1303, 1365, 1659, 1885, 2076, 2340, 2358, 2589 |
| DpnII | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| DraI | TTTAAA | 3 cut(s) | 790, 1425, 1911 |
| Eam1104I | CTCTTC | 9 cut(s) | 38, 145, 234, 247, 696, 1410, 2196, 2382, 2663 |
| EarI | CTCTTC | 9 cut(s) | 38, 145, 234, 247, 696, 1410, 2196, 2382, 2663 |
| Ecl136II | GAGCTC | 1 cut(s) | 1354 |
| Eco130I | CCWWGG | 3 cut(s) | 1700, 1977, 2111 |
| Eco147I | AGGCCT | 1 cut(s) | 2110 |
| Eco24I | GRGCYC | 3 cut(s) | 269, 1123, 1356 |
| Eco47I | GGWCC | 7 cut(s) | 92, 527, 605, 795, 1277, 2177, 2570 |
| Eco53kI | GAGCTC | 1 cut(s) | 1354 |
| Eco57I | CTGAAG | 1 cut(s) | 1344 |
| Eco81I | CCTNAGG | 1 cut(s) | 1567 |
| EcoICRI | GAGCTC | 1 cut(s) | 1354 |
| EcoO109I | RGGNCCY | 2 cut(s) | 92, 266 |
| EcoRI | GAATTC | 3 cut(s) | 373, 610, 2057 |
| EcoRII | CCWGG | 3 cut(s) | 1346, 1380, 2158 |
| EcoT14I | CCWWGG | 3 cut(s) | 1700, 1977, 2111 |
| EcoT38I | GRGCYC | 3 cut(s) | 269, 1123, 1356 |
| ErhI | CCWWGG | 3 cut(s) | 1700, 1977, 2111 |
| Esp3I | CGTCTC | 1 cut(s) | 317 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1419, 1451, 2334, 2366 |
| FaqI | GGGAC | 3 cut(s) | 808, 830, 2607 |
| FauNDI | CATATG | 1 cut(s) | 981 |
| FbaI | TGATCA | 2 cut(s) | 2338, 2356 |
| FblI | GTMKAC | 1 cut(s) | 2470 |
| Fnu4HI | GCNGC | 7 cut(s) | 1457, 1646, 2128, 2131, 2284, 2287, 2445 |
| FokI | GGATG | 4 cut(s) | 864, 1202, 1784, 2530 |
| FriOI | GRGCYC | 3 cut(s) | 269, 1123, 1356 |
| Fsp4HI | GCNGC | 7 cut(s) | 1457, 1646, 2128, 2131, 2284, 2287, 2445 |
| FspI | TGCGCA | 1 cut(s) | 1091 |
| GlaI | GCGC | 3 cut(s) | 1091, 1434, 1460 |
| GluI | GCNGC | 7 cut(s) | 1457, 1646, 2128, 2131, 2284, 2287, 2445 |
| GsaI | CCCAGC | 1 cut(s) | 1481 |
| HaeIII | GGCC | 3 cut(s) | 267, 1207, 2110 |
| HapII | CCGG | 4 cut(s) | 366, 1106, 2180, 2438 |
| HhaI | GCGC | 3 cut(s) | 1092, 1435, 1461 |
| Hin6I | GCGC | 3 cut(s) | 1090, 1433, 1459 |
| HinP1I | GCGC | 3 cut(s) | 1090, 1433, 1459 |
| HincII | GTYRAC | 1 cut(s) | 535 |
| HindII | GTYRAC | 1 cut(s) | 535 |
| HpaI | GTTAAC | 1 cut(s) | 535 |
| HpaII | CCGG | 4 cut(s) | 366, 1106, 2180, 2438 |
| HphI | GGTGA | 3 cut(s) | 204, 380, 2174 |
| Hpy166II | GTNNAC | 5 cut(s) | 436, 535, 1495, 2035, 2471 |
| Hpy8I | GTNNAC | 5 cut(s) | 436, 535, 1495, 2035, 2471 |
| HpyAV | CCTTC | 9 cut(s) | 90, 268, 332, 506, 1180, 1218, 1290, 1368, 2635 |
| HpyCH4III | ACNGT | 4 cut(s) | 311, 1067, 1164, 1374 |
| HpyCH4IV | ACGT | 1 cut(s) | 901 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1102, 1111, 1386, 2116, 2372 |
| HpyF3I | CTNAG | 9 cut(s) | 919, 1328, 1440, 1567, 1794, 2082, 2279, 2648, 2680 |
| HpySE526I | ACGT | 1 cut(s) | 901 |
| HspAI | GCGC | 3 cut(s) | 1090, 1433, 1459 |
| Ksp22I | TGATCA | 2 cut(s) | 2338, 2356 |
| KspAI | GTTAAC | 1 cut(s) | 535 |
| Kzo9I | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| LmnI | GCTCC | 6 cut(s) | 229, 1003, 1324, 1351, 1359, 1981 |
| Lsp1109I | GCAGC | 5 cut(s) | 1468, 1632, 2142, 2298, 2456 |
| LweI | GCATC | 8 cut(s) | 886, 898, 1092, 1137, 1420, 1607, 1762, 2605 |
| MabI | ACCWGGT | 1 cut(s) | 2158 |
| MaeII | ACGT | 1 cut(s) | 901 |
| MaeIII | GTNAC | 4 cut(s) | 949, 1071, 1854, 1971 |
| MalI | GATC | 8 cut(s) | 1303, 1365, 1659, 1885, 2076, 2340, 2358, 2589 |
| MbiI | CCGCTC | 1 cut(s) | 2721 |
| MboI | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| MfeI | CAATTG | 2 cut(s) | 1902, 1991 |
| MflI | RGATCY | 2 cut(s) | 1363, 2074 |
| MhlI | GDGCHC | 3 cut(s) | 269, 1123, 1356 |
| MlyI | GAGTC | 3 cut(s) | 694, 2396, 2645 |
| MmeI | TCCRAC | 3 cut(s) | 574, 662, 2548 |
| MroXI | GAANNNNTTC | 1 cut(s) | 321 |
| MslI | CAYNNNNRTG | 2 cut(s) | 984, 1201 |
| MspA1I | CMGCKG | 1 cut(s) | 2289 |
| MspI | CCGG | 4 cut(s) | 366, 1106, 2180, 2438 |
| MspR9I | CCNGG | 3 cut(s) | 1348, 1382, 2160 |
| MunI | CAATTG | 2 cut(s) | 1902, 1991 |
| Mva1269I | GAATGC | 2 cut(s) | 1391, 1868 |
| MvaI | CCWGG | 3 cut(s) | 1348, 1382, 2160 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1102, 1111, 1386, 2116, 2372 |
| NcoI | CCATGG | 1 cut(s) | 1977 |
| NdeI | CATATG | 1 cut(s) | 981 |
| NdeII | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| NlaIV | GGNNCC | 5 cut(s) | 93, 267, 796, 1800, 2178 |
| NmuCI | GTSAC | 1 cut(s) | 949 |
| NsbI | TGCGCA | 1 cut(s) | 1091 |
| NspI | RCATGY | 2 cut(s) | 677, 695 |
| PagI | TCATGA | 2 cut(s) | 1834, 2212 |
| PceI | AGGCCT | 1 cut(s) | 2110 |
| PctI | GAATGC | 2 cut(s) | 1391, 1868 |
| PdmI | GAANNNNTTC | 1 cut(s) | 321 |
| PfeI | GAWTC | 8 cut(s) | 218, 413, 490, 752, 1214, 1607, 1628, 2732 |
| PinAI | ACCGGT | 1 cut(s) | 365 |
| PkrI | GCNGC | 7 cut(s) | 1458, 1647, 2129, 2132, 2285, 2288, 2446 |
| PleI | GAGTC | 3 cut(s) | 693, 2396, 2644 |
| PpsI | GAGTC | 3 cut(s) | 693, 2396, 2644 |
| PpuMI | RGGWCCY | 1 cut(s) | 92 |
| PsiI | TTATAA | 2 cut(s) | 849, 2490 |
| Psp124BI | GAGCTC | 1 cut(s) | 1356 |
| Psp5II | RGGWCCY | 1 cut(s) | 92 |
| Psp6I | CCWGG | 3 cut(s) | 1346, 1380, 2158 |
| PspFI | CCCAGC | 1 cut(s) | 1477 |
| PspGI | CCWGG | 3 cut(s) | 1346, 1380, 2158 |
| PspN4I | GGNNCC | 5 cut(s) | 93, 267, 796, 1800, 2178 |
| PspOMI | GGGCCC | 1 cut(s) | 265 |
| PspPI | GGNCC | 9 cut(s) | 92, 265, 266, 527, 605, 795, 1277, 2177, 2570 |
| PspPPI | RGGWCCY | 1 cut(s) | 92 |
| PstNI | CAGNNNCTG | 1 cut(s) | 221 |
| PsuI | RGATCY | 2 cut(s) | 1363, 2074 |
| PvuII | CAGCTG | 1 cut(s) | 2289 |
| RsaI | GTAC | 6 cut(s) | 278, 566, 672, 1715, 2070, 2184 |
| RsaNI | GTAC | 6 cut(s) | 277, 565, 671, 1714, 2069, 2183 |
| RseI | CAYNNNNRTG | 2 cut(s) | 984, 1201 |
| SacI | GAGCTC | 1 cut(s) | 1356 |
| SatI | GCNGC | 7 cut(s) | 1457, 1646, 2128, 2131, 2284, 2287, 2445 |
| Sau3AI | GATC | 8 cut(s) | 1301, 1363, 1657, 1883, 2074, 2338, 2356, 2587 |
| Sau96I | GGNCC | 9 cut(s) | 92, 265, 266, 527, 605, 795, 1277, 2177, 2570 |
| SchI | GAGTC | 3 cut(s) | 694, 2396, 2645 |
| ScrFI | CCNGG | 3 cut(s) | 1348, 1382, 2160 |
| SduI | GDGCHC | 3 cut(s) | 269, 1123, 1356 |
| SexAI | ACCWGGT | 1 cut(s) | 2158 |
| SfaNI | GCATC | 8 cut(s) | 886, 898, 1092, 1137, 1420, 1607, 1762, 2605 |
| SfcI | CTRYAG | 1 cut(s) | 1735 |
| SinI | GGWCC | 7 cut(s) | 92, 527, 605, 795, 1277, 2177, 2570 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 984, 1201 |
| SmlI | CTYRAG | 1 cut(s) | 1803 |
| SmoI | CTYRAG | 1 cut(s) | 1803 |
| SpeI | ACTAGT | 1 cut(s) | 1028 |
| SseBI | AGGCCT | 1 cut(s) | 2110 |
| SsiI | CCGC | 5 cut(s) | 689, 1866, 2128, 2284, 2719 |
| SspI | AATATT | 3 cut(s) | 87, 346, 1842 |
| SstI | GAGCTC | 1 cut(s) | 1356 |
| StuI | AGGCCT | 1 cut(s) | 2110 |
| StyD4I | CCNGG | 3 cut(s) | 1346, 1380, 2158 |
| StyI | CCWWGG | 3 cut(s) | 1700, 1977, 2111 |
| TaaI | ACNGT | 4 cut(s) | 311, 1067, 1164, 1374 |
| TaiI | ACGT | 1 cut(s) | 904 |
| TaqI | TCGA | 6 cut(s) | 315, 396, 416, 1131, 1184, 2639 |
| TaqII | GACCGA | 1 cut(s) | 1862 |
| TatI | WGTACW | 3 cut(s) | 276, 564, 670 |
| TauI | GCSGC | 2 cut(s) | 2130, 2286 |
| TfiI | GAWTC | 8 cut(s) | 218, 413, 490, 752, 1214, 1607, 1628, 2732 |
| TscAI | CASTG | 1 cut(s) | 1554 |
| TseFI | GTSAC | 1 cut(s) | 949 |
| TseI | GCWGC | 5 cut(s) | 1456, 1645, 2130, 2286, 2444 |
| Tsp45I | GTSAC | 1 cut(s) | 949 |
| TspGWI | ACGGA | 1 cut(s) | 1001 |
| TspRI | CASTG | 1 cut(s) | 1554 |
| VpaK11BI | GGWCC | 7 cut(s) | 92, 527, 605, 795, 1277, 2177, 2570 |
| XbaI | TCTAGA | 2 cut(s) | 760, 2519 |
| XceI | RCATGY | 2 cut(s) | 677, 695 |
| XmaJI | CCTAGG | 2 cut(s) | 1700, 2111 |
| XmiI | GTMKAC | 1 cut(s) | 2470 |
| XmnI | GAANNNNTTC | 1 cut(s) | 321 |