pycom04g02250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014015
Physical Location & Seq
Forward (+)
2007171 .. 2009807
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g02250.1

Sequence Viewer

Length: 2637 bp
ATGAACATAATCCCAGAGGAATGGAAATCTCTCTTCCCAATCTCATCAGTCTTCAAGCCTCCTCTCCTCCTCTCAAACCCTTCACTTAAACCCATCCTCGGTCCCCTCATCTTCAACCCTAAACCCAACTCCGCCGCCATCCTCTTCTCTTCCTCCTGCTTCCTCCCCCATCTCCCTCCTCCACCCCACCTCTCTCTCCCCCGTTTCCTCCTCACCTCCTCCCCAGACTCCGCTCCTCTTCCCTCCACCTCCCATTCCATCGCCTCTCTATTTGGCCCCCACAACCACAAACACGACGTCGTCTCTTCCCTCCTCCACAACCGCCTTGAGCTCCTCCAGTGCCCGCAGATTAACACCATTGTTGTTTTCTTCCCCACTGGCCAGAACTCTGACCAAGTTGGGTTCTTGCAGTTGGTGCTAAAAGATTCGACCTTTGGTGTCAAAGCTGATGAAAGCGGTGAGGTTTTGGGTTTGAGGGGTCCATCCAATTACCGGATTTCGAGGATTTCAGTGAACCCGGTTCCAGGGTTTTCAAGTTCAAGAGGTAATGATTCTTCTGTTGTAATTGGGTATTTGTTGGCTTCTACTATGCACTCTGTCCAGTGGTTTACTGTAAAGGTTGGAGATACAAGTTCGAATTTGGGTAATAAAGTTAGTTTAAGTTACTTGGGCAGTAAGGTTTTCAAGACTTGCTGTGTTGTACATGCCTCTTGGAGTCCACATTTACAGGAAGAGAGTGTGGTTTTGTTAGAAAATGGTGCTTTGTTTTTGTTTGATTTGGAGTCTCGTCCGAAAGCCCATAATTTTAAGTTTAAAGGGACCAGATTGAAAGTAGTATGGGATAATGGTGATGTTTCGAGTACTTCAAGAAATTATAGGTGGTTGAGTTGTGAATTCAGTTGGCATCCTAGAGTTTTGGTCATTGCGCGTTCGGATGCAGTTTTCTTAGTTGATTTGAGGTCCTGTGAATGTAGTGTGACTTGTCTGATGAAGATTGAGATGTTGCATATGTATGTCCCGGTTGAAAAGGAGCAGTTCCTTGTGCTTTCAAGGACTGCTTCTGATGATTTTCACTTTGTTTTAGCTTCTGACACGTTATTACTTCTTTGTGATGTGCGCAAACCATTGATGCCGGTGTTGCAATGGGCTCATGGCCTTGATAAACCAAGCTATCTTGATGTGTTTAGACTGTCAGAATTGAGGTCACACTTGAGGGAGGACATGTATAAGTGGGCTTCTGACTCGGGTTTTTGCATTGTGATGGGGTCATTTTGGAATTGTCAGTTTAATACCTTTTGCTATGGCCCTTCCCTCCCAACTCCAATAGGATCAGTTGCTTCCAAAGTAGCAGAACTTCGGAAGTCATTTTATGCATGGGAGCTACCTTCAGATGTTTTACTGCCGGGCCACGAGTGCCATTGTGGAAACTGTATATTAAGAGAAGAGTTTGTAAAAGACGCTCTTCCTGAATGGGTTGATTTGCAACAGAAGAAAGAAATAGTTTTGGGTTTTGGCATTGTAAACAAAGAATTGTCTTCTCTGCTTTCTGAGCCAGATGAATTCGGTGGTTTTACATTGATCAGGCTTATGTCCTCGGGGAAGCTAGAATTACAGAGATACTGTGCATCGTGGGATCCTGTAAAAAAGGTAGAAGGATCGCATAGGGAATTGCTGCATTTTAAGGATTATTTTCTGGATTCCCTAGCTGATGAGGAGTACAAATTCCCTAGGAGATTTAATTACCTTAAACTGGACTACCTCTGTGCTTATCTAAATGATAAGCTTGATGAAGTCTTAGATACAAAAATGAAAGTTCCCTCTAAGATTGTTCAAGGGACGGAACTTTTTAGCCCAGAATACCATGAGCTTTTATGTAAAAAGTTACGGGCTTGTGGTTTTGGTCAGTTTAGATCATCTCCTGCGGTCACAAGTGTTTTAAATGACATCAGCTTGCCAACAAGCATACATGAAGTAGTTTTGAAGAGATTGTGGTCAGAATTACCAATGGAACTTCTGCAACTGGCCTTTTCTAACTACCCTGAAATCCTTGAAGTGCTTGTGGACGAAAAGAGGTCGGCTTTGGAATTCTCTGCCGTACCGGACCAATCCCAGTTGCCTCCTTTTATTTTAAGGAAGCCTTCATGCCGCAGCAGTAAGTGGTCACAGAAAGTGAAACCCGGTGATGCCCTTGTCGGTCCAGTTCTTCCTCTTCCTATATTGCTTACACTTCACGAACTTCGCAATGGGTGTCTGAATTCGCAAGATGAACAGTCAGGTAAATTTTCAGTAGAGGCAGAAATTAGTCGCAGCTGTAATAAGATCATGCAGGTCGCCAGCGAAATGACTGTTTCTAAACCGGACCTTGAGATTGTTGATGAACAAGCGGCATCCCTAGCTAATGATGGAGAGGAGACATGGAGATGCACTCAACAACCGAAACCTTTCTTTTCATACCAGCCAGTTGCAGGTACAGGATCTCCAATGGACCATATACGGGACAAATCTGTCTTTAAGGATGACAGATTTGACACCGTAATTTCTAAAGTGTCTGAGAAGAATCCTGCTCCCAACGGTAGTGAGGATAACGTCGGACTGGAACTGTTTGATCGATCTTTGTCCAGTAGAGTTGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

879

Amino Acids

98.59

Weight (kDa)

6.09

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF1C_beta-prop PF20641 236 - 332 1.8e-06 TAF1C, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18310
fragaria_vesca FvH4_5g25850
malus_domestica MD04G1026400.v1.1
prunus_persica Prupe.2G050600_v2.0.a1
pyrus_communis pycom04g02250
rosa_chinensis RchiOBHm_Chr6g0264721 RchiOBHm_Chr6g0264731 RchiOBHm_Chr7g0216331
rosa_laevigata RLG00000002581
rosa_multiflora Rmu_sc0015060.1_g000019
rosa_roxburghii Rroxscaffold_3G00243320
rosa_rugosa Rorug07G0160000
rosa_samantha Rh7AG297400 Rh7BG289700 Rh7CG317600 Rh7DG299800
rosa_wichuraiana Rw7G025300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2504
AatII GACGTC 1 cut(s) 300
Acc16I TGCGCA 1 cut(s) 1118
Acc36I ACCTGC 2 cut(s) 2317, 2456
AccBSI CCGCTC 1 cut(s) 233
AccII CGCG 1 cut(s) 928
AciI CCGC 9 cut(s) 132, 135, 231, 322, 344, 456, 1922, 2146, 2384
AclWI GGATC 5 cut(s) 1336, 1628, 1641, 1663, 2482
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 7 cut(s) 637, 893, 1559, 1721, 2084, 2254, 2279
AcuI CTGAAG 1 cut(s) 1371
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 5 cut(s) 702, 862, 1718, 2097, 2470
AfiI CCNNNNNNNGG 6 cut(s) 98, 492, 524, 1750, 2465, 2494
AflIII ACRYGT 2 cut(s) 1092, 1221
AjnI CCWGG 1 cut(s) 523
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 3 cut(s) 307, 789, 2405
AlwI GGATC 5 cut(s) 1336, 1628, 1641, 1663, 2482
Ama87I CYCGRG 2 cut(s) 1243, 1594
AoxI GGCC 6 cut(s) 274, 379, 1153, 1303, 1405, 2022
ApeKI GCWGC 3 cut(s) 1672, 2148, 2307
ApoI RAATTY 7 cut(s) 637, 893, 1559, 1721, 2084, 2254, 2279
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
Asp700I GAANNNNTTC 1 cut(s) 2441
AspA2I CCTAGG 1 cut(s) 1727
AspLEI GCGC 2 cut(s) 928, 1119
AsuC2I CCSGG 4 cut(s) 518, 1019, 1404, 2178
AsuHPI GGTGA 4 cut(s) 205, 470, 860, 2192
AsuII TTCGAA 1 cut(s) 635
AvaI CYCGRG 2 cut(s) 1243, 1594
AvaII GGWCC 8 cut(s) 101, 479, 819, 960, 2101, 2195, 2359, 2485
AvrII CCTAGG 1 cut(s) 1727
BaeGI GKGCMC 1 cut(s) 344
BalI TGGCCA 1 cut(s) 381
BamHI GGATCC 1 cut(s) 1633
BanII GRGCYC 2 cut(s) 333, 1150
BarI GAAGNNNNNNTAC 4 cut(s) 538, 570, 1338, 1370
BauI CACGAG 1 cut(s) 1409
BbsI GAAGAC 2 cut(s) 43, 1527
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 1659, 2160, 2319
BccI CCATC 7 cut(s) 101, 146, 177, 266, 490, 1255, 2396
BceAI ACGGC 1 cut(s) 2078
BciT130I CCWGG 1 cut(s) 525
BclI TGATCA 1 cut(s) 1578
BcnI CCSGG 4 cut(s) 518, 1019, 1404, 2178
BcoDI GTCTC 3 cut(s) 307, 789, 2405
BfaI CTAG 5 cut(s) 909, 1604, 1703, 1728, 2393
BfuAI ACCTGC 2 cut(s) 2317, 2456
BisI GCNGC 6 cut(s) 135, 1673, 2146, 2149, 2308, 2385
BlnI CCTAGG 1 cut(s) 1727
BlsI GCNGC 6 cut(s) 136, 1674, 2147, 2150, 2309, 2386
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 5 cut(s) 518, 525, 1019, 1404, 2178
Bme18I GGWCC 8 cut(s) 101, 479, 819, 960, 2101, 2195, 2359, 2485
BmeT110I CYCGRG 2 cut(s) 1243, 1594
BmiI GGNNCC 6 cut(s) 103, 277, 480, 522, 820, 1635
BmrFI CCNGG 5 cut(s) 518, 525, 1019, 1404, 2178
BmrI ACTGGG 1 cut(s) 2104
BmsI GCATC 7 cut(s) 913, 925, 1119, 1634, 2173, 2396, 2411
BmuI ACTGGG 1 cut(s) 2104
BpiI GAAGAC 2 cut(s) 43, 1527
BplI GAGNNNNNCTC 2 cut(s) 2410, 2442
BpmI CTGGAG 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 3 cut(s) 347, 1231, 2384
BpuMI CCSGG 4 cut(s) 518, 1019, 1404, 2178
Bsa29I ATCGAT 1 cut(s) 2608
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 4 cut(s) 97, 524, 1593, 1727
BsaWI WCCGGW 3 cut(s) 492, 2098, 2356
BsaXI ACNNNNNCTCC 4 cut(s) 1208, 1238, 2461, 2491
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 492, 524, 1750, 2465, 2494
Bse118I RCCGGY 1 cut(s) 1132
Bse3DI GCAATG 3 cut(s) 921, 1148, 2248
BseBI CCWGG 1 cut(s) 525
BseCI ATCGAT 1 cut(s) 2608
BseDI CCNNGG 4 cut(s) 97, 524, 1593, 1727
BseGI GGATG 7 cut(s) 93, 138, 482, 904, 940, 2387, 2521
BseLI CCNNNNNNNGG 6 cut(s) 98, 492, 524, 1750, 2465, 2494
BseMI GCAATG 3 cut(s) 921, 1148, 2248
BseMII CTCAG 2 cut(s) 1539, 2541
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 3 cut(s) 1659, 2160, 2319
Bsh1236I CGCG 1 cut(s) 928
BshFI GGCC 6 cut(s) 276, 381, 1155, 1305, 1407, 2024
BshVI ATCGAT 1 cut(s) 2608
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 1243, 1594
BsiSI CCGG 8 cut(s) 493, 518, 1019, 1133, 1403, 2099, 2178, 2357
BslFI GGGAC 5 cut(s) 87, 832, 1001, 1849, 2510
BslI CCNNNNNNNGG 6 cut(s) 98, 492, 524, 1750, 2465, 2494
BsmAI GTCTC 3 cut(s) 307, 789, 2405
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 5 cut(s) 87, 832, 1001, 1849, 2510
BsnI GGCC 6 cut(s) 276, 381, 1155, 1305, 1407, 2024
BsoBI CYCGRG 2 cut(s) 1243, 1594
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 333, 344, 1150
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
BspACI CCGC 9 cut(s) 132, 135, 231, 322, 344, 456, 1922, 2146, 2384
BspANI GGCC 6 cut(s) 276, 381, 1155, 1305, 1407, 2024
BspCNI CTCAG 2 cut(s) 1540, 2542
BspDI ATCGAT 1 cut(s) 2608
BspFNI CGCG 1 cut(s) 928
BspLI GGNNCC 6 cut(s) 103, 277, 480, 522, 820, 1635
BspMI ACCTGC 2 cut(s) 2317, 2456
BspPI GGATC 5 cut(s) 1336, 1628, 1641, 1663, 2482
BspQI GCTCTTC 1 cut(s) 1467
BspT104I TTCGAA 1 cut(s) 635
BsrBI CCGCTC 1 cut(s) 233
BsrDI GCAATG 3 cut(s) 921, 1148, 2248
BsrFI RCCGGY 1 cut(s) 1132
BsrGI TGTACA 1 cut(s) 700
BssAI RCCGGY 1 cut(s) 1132
BssECI CCNNGG 4 cut(s) 97, 524, 1593, 1727
BssMI GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 1409
BssT1I CCWWGG 1 cut(s) 1727
Bst2BI CACGAG 1 cut(s) 1409
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 9 cut(s) 613, 1191, 1430, 1622, 2271, 2347, 2533, 2573, 2601
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 415
BstAUI TGTACA 1 cut(s) 700
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 344, 1952, 2335
BstDEI CTNAG 5 cut(s) 946, 1548, 1795, 1821, 2550
BstF5I GGATG 7 cut(s) 93, 138, 482, 904, 940, 2387, 2521
BstFNI CGCG 1 cut(s) 928
BstHHI GCGC 2 cut(s) 928, 1119
BstKTI GATC 9 cut(s) 1331, 1581, 1636, 1658, 1913, 2322, 2477, 2608, 2612
BstMAI GTCTC 3 cut(s) 307, 789, 2405
BstMBI GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
BstMWI GCNNNNNNNGC 5 cut(s) 415, 1138, 1413, 1549, 2393
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 2 cut(s) 707, 1225
BstSCI CCNGG 5 cut(s) 516, 523, 1017, 1402, 2176
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 1 cut(s) 928
BstV1I GCAGC 3 cut(s) 1659, 2160, 2319
BstV2I GAAGAC 2 cut(s) 43, 1527
BstX2I RGATCY 2 cut(s) 1633, 2474
BstXI CCANNNNNNTGG 1 cut(s) 21
BstYI RGATCY 2 cut(s) 1633, 2474
Bsu15I ATCGAT 1 cut(s) 2608
BsuRI GGCC 6 cut(s) 276, 381, 1155, 1305, 1407, 2024
BsuTUI ATCGAT 1 cut(s) 2608
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 7 cut(s) 93, 138, 482, 904, 940, 2387, 2521
BtsIMutI CAGTG 4 cut(s) 344, 375, 516, 608
BveI ACCTGC 2 cut(s) 2317, 2456
Cac8I GCNNGC 3 cut(s) 344, 1952, 2335
CfoI GCGC 2 cut(s) 928, 1119
Cfr10I RCCGGY 1 cut(s) 1132
ClaI ATCGAT 1 cut(s) 2608
CseI GACGC 1 cut(s) 1466
Csp6I GTAC 5 cut(s) 701, 861, 1717, 2096, 2469
CviAII CATG 9 cut(s) 704, 1151, 1222, 1374, 1862, 1967, 2142, 2323, 2415
CviQI GTAC 5 cut(s) 701, 861, 1717, 2096, 2469
DdeI CTNAG 5 cut(s) 946, 1548, 1795, 1821, 2550
DpnI GATC 9 cut(s) 1330, 1580, 1635, 1657, 1912, 2321, 2476, 2607, 2611
DpnII GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
DraI TTTAAA 2 cut(s) 814, 1938
DrdI GACNNNNNNGTC 1 cut(s) 2504
DseDI GACNNNNNNGTC 1 cut(s) 2504
EaeI YGGCCR 1 cut(s) 379
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 1727
Eco24I GRGCYC 2 cut(s) 333, 1150
Eco47I GGWCC 8 cut(s) 101, 479, 819, 960, 2101, 2195, 2359, 2485
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 1371
Eco88I CYCGRG 2 cut(s) 1243, 1594
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 960
EcoRI GAATTC 4 cut(s) 893, 1559, 2084, 2254
EcoRII CCWGG 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 1727
EcoT22I ATGCAT 1 cut(s) 1375
EcoT38I GRGCYC 2 cut(s) 333, 1150
ErhI CCWWGG 1 cut(s) 1727
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 9 cut(s) 707, 1154, 1225, 1377, 1865, 1970, 2145, 2326, 2418
FalI AAGNNNNNCTT 6 cut(s) 1042, 1074, 1446, 1478, 2122, 2154
FaqI GGGAC 5 cut(s) 87, 832, 1001, 1849, 2510
FatI CATG 9 cut(s) 703, 1150, 1221, 1373, 1861, 1966, 2141, 2322, 2414
FauI CCCGC 1 cut(s) 351
FauNDI CATATG 1 cut(s) 1008
FbaI TGATCA 1 cut(s) 1578
Fnu4HI GCNGC 6 cut(s) 135, 1673, 2146, 2149, 2308, 2385
FokI GGATG 7 cut(s) 80, 125, 469, 891, 947, 2374, 2528
FriOI GRGCYC 2 cut(s) 333, 1150
Fsp4HI GCNGC 6 cut(s) 135, 1673, 2146, 2149, 2308, 2385
FspBI CTAG 5 cut(s) 909, 1604, 1703, 1728, 2393
FspI TGCGCA 1 cut(s) 1118
GlaI GCGC 2 cut(s) 927, 1118
GluI GCNGC 6 cut(s) 135, 1673, 2146, 2149, 2308, 2385
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 6 cut(s) 276, 381, 1155, 1305, 1407, 2024
HapII CCGG 8 cut(s) 493, 518, 1019, 1133, 1403, 2099, 2178, 2357
HgaI GACGC 1 cut(s) 1466
HhaI GCGC 2 cut(s) 928, 1119
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 9 cut(s) 707, 1154, 1225, 1377, 1865, 1970, 2145, 2326, 2418
Hin6I GCGC 2 cut(s) 926, 1117
HinP1I GCGC 2 cut(s) 926, 1117
HindIII AAGCTT 1 cut(s) 1781
HinfI GANTC 8 cut(s) 227, 425, 551, 715, 782, 1241, 1697, 2557
HpaII CCGG 8 cut(s) 493, 518, 1019, 1133, 1403, 2099, 2178, 2357
HphI GGTGA 4 cut(s) 205, 470, 860, 2192
Hpy166II GTNNAC 5 cut(s) 514, 609, 719, 1522, 2062
Hpy188III TCNNGA 7 cut(s) 540, 685, 867, 1175, 1466, 1694, 2231
Hpy8I GTNNAC 5 cut(s) 514, 609, 719, 1522, 2062
Hpy99I CGWCG 3 cut(s) 299, 302, 2591
HpyAV CCTTC 5 cut(s) 90, 1317, 1395, 1646, 2148
HpyCH4III ACNGT 9 cut(s) 613, 1191, 1430, 1622, 2271, 2347, 2533, 2573, 2601
HpyCH4IV ACGT 3 cut(s) 297, 1094, 2586
HpyF10VI GCNNNNNNNGC 5 cut(s) 415, 1138, 1413, 1549, 2393
HpyF3I CTNAG 5 cut(s) 946, 1548, 1795, 1821, 2550
HpySE526I ACGT 3 cut(s) 297, 1094, 2586
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 9 cut(s) 707, 1154, 1225, 1377, 1865, 1970, 2145, 2326, 2418
HspAI GCGC 2 cut(s) 926, 1117
Ksp22I TGATCA 1 cut(s) 1578
Kzo9I GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
LguI GCTCTTC 1 cut(s) 1467
LmnI GCTCC 5 cut(s) 238, 336, 1030, 1378, 2569
Lsp1109I GCAGC 3 cut(s) 1659, 2160, 2319
LweI GCATC 7 cut(s) 913, 925, 1119, 1634, 2173, 2396, 2411
MaeI CTAG 5 cut(s) 909, 1604, 1703, 1728, 2393
MaeII ACGT 3 cut(s) 297, 1094, 2586
MaeIII GTNAC 6 cut(s) 662, 976, 1203, 1881, 1924, 2160
MalI GATC 9 cut(s) 1330, 1580, 1635, 1657, 1912, 2321, 2476, 2607, 2611
MbiI CCGCTC 1 cut(s) 233
MboI GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
MflI RGATCY 2 cut(s) 1633, 2474
MhlI GDGCHC 3 cut(s) 333, 344, 1150
MlsI TGGCCA 1 cut(s) 381
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 4 cut(s) 221, 724, 791, 1235
MmeI TCCRAC 2 cut(s) 601, 2569
Mox20I TGGCCA 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 1375
MroXI GAANNNNTTC 1 cut(s) 2441
MscI TGGCCA 1 cut(s) 381
MslI CAYNNNNRTG 3 cut(s) 1011, 1259, 2419
Msp20I TGGCCA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 2310
MspI CCGG 8 cut(s) 493, 518, 1019, 1133, 1403, 2099, 2178, 2357
MspR9I CCNGG 5 cut(s) 518, 525, 1019, 1404, 2178
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 1 cut(s) 928
MwoI GCNNNNNNNGC 5 cut(s) 415, 1138, 1413, 1549, 2393
NciI CCSGG 4 cut(s) 518, 1019, 1404, 2178
NdeI CATATG 1 cut(s) 1008
NdeII GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
NlaIII CATG 9 cut(s) 707, 1154, 1225, 1377, 1865, 1970, 2145, 2326, 2418
NlaIV GGNNCC 6 cut(s) 103, 277, 480, 522, 820, 1635
NmuCI GTSAC 4 cut(s) 976, 1203, 1924, 2160
NsbI TGCGCA 1 cut(s) 1118
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 2 cut(s) 707, 1225
NspV TTCGAA 1 cut(s) 635
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 1467
PdmI GAANNNNTTC 1 cut(s) 2441
PfeI GAWTC 4 cut(s) 425, 551, 1697, 2557
PflFI GACNNNGTC 1 cut(s) 299
PkrI GCNGC 6 cut(s) 136, 1674, 2147, 2150, 2309, 2386
PleI GAGTC 4 cut(s) 221, 723, 790, 1235
PpsI GAGTC 4 cut(s) 221, 723, 790, 1235
PpuMI RGGWCCY 1 cut(s) 960
PscI ACATGT 1 cut(s) 1221
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 960
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 6 cut(s) 103, 277, 480, 522, 820, 1635
PspPPI RGGWCCY 1 cut(s) 960
PsuI RGATCY 2 cut(s) 1633, 2474
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 2310
RsaI GTAC 5 cut(s) 702, 862, 1718, 2097, 2470
RsaNI GTAC 5 cut(s) 701, 861, 1717, 2096, 2469
RseI CAYNNNNRTG 3 cut(s) 1011, 1259, 2419
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 1467
SatI GCNGC 6 cut(s) 135, 1673, 2146, 2149, 2308, 2385
Sau3AI GATC 9 cut(s) 1328, 1578, 1633, 1655, 1910, 2319, 2474, 2605, 2609
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 4 cut(s) 221, 724, 791, 1235
ScrFI CCNGG 5 cut(s) 518, 525, 1019, 1404, 2178
SduI GDGCHC 3 cut(s) 333, 344, 1150
SfaNI GCATC 7 cut(s) 913, 925, 1119, 1634, 2173, 2396, 2411
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 8 cut(s) 101, 479, 819, 960, 2101, 2195, 2359, 2485
SmiMI CAYNNNNRTG 3 cut(s) 1011, 1259, 2419
SmlI CTYRAG 3 cut(s) 326, 1210, 2363
SmoI CTYRAG 3 cut(s) 326, 1210, 2363
SsiI CCGC 9 cut(s) 132, 135, 231, 322, 344, 456, 1922, 2146, 2384
SspMI CTAG 5 cut(s) 909, 1604, 1703, 1728, 2393
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 5 cut(s) 516, 523, 1017, 1402, 2176
StyI CCWWGG 1 cut(s) 1727
TaaI ACNGT 9 cut(s) 613, 1191, 1430, 1622, 2271, 2347, 2533, 2573, 2601
TaiI ACGT 3 cut(s) 300, 1097, 2589
TaqI TCGA 5 cut(s) 428, 500, 635, 857, 2608
TaqII GACCGA 2 cut(s) 89, 2183
TatI WGTACW 3 cut(s) 700, 860, 1716
TauI GCSGC 3 cut(s) 137, 2148, 2387
TfiI GAWTC 4 cut(s) 425, 551, 1697, 2557
TscAI CASTG 4 cut(s) 344, 382, 516, 608
TseFI GTSAC 4 cut(s) 976, 1203, 1924, 2160
TseI GCWGC 3 cut(s) 1672, 2148, 2307
Tsp45I GTSAC 4 cut(s) 976, 1203, 1924, 2160
TspGWI ACGGA 1 cut(s) 1853
TspRI CASTG 4 cut(s) 344, 382, 516, 608
Tth111I GACNNNGTC 1 cut(s) 299
VpaK11BI GGWCC 8 cut(s) 101, 479, 819, 960, 2101, 2195, 2359, 2485
XapI RAATTY 7 cut(s) 637, 893, 1559, 1721, 2084, 2254, 2279
XceI RCATGY 2 cut(s) 707, 1225
XmaJI CCTAGG 1 cut(s) 1727
XmnI GAANNNNTTC 1 cut(s) 2441
XspI CTAG 5 cut(s) 909, 1604, 1703, 1728, 2393
ZraI GACGTC 1 cut(s) 298
ZrmI AGTACT 1 cut(s) 862
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.