Prupe.2G196300_v2.0.a1

Pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0011389
Physical Location & Seq
Reverse (-)
23365023 .. 23369401
4379 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G196300.1

Sequence Viewer

Length: 1392 bp
ATGCATCTGCTCTTTCAGCGGTCCTCAAAAGCCGCAGTAACGGTCACTCGCTCTCTAGCCCTCCCGCCAAAACCCCCATCCTCCTCCTCCTCCTCCTCCGTCTTCTTCCTCTCATCACAACCCCATAACCAGAACCACGAAATCGGCTCAATCGGCTCCCCTTCCAGGATCCAAAATCTCATCGCTTCACAATCAGACCCTCTCCTCGCCAAAGAGATCTTCGACTTGGCCGCCCGCCAACCCCATTTTCGCCACTCCTACTCTTCCTTCTTCACTCTCATCCTCAAGCTCGGCCGCTCCAAGTACTTCTCTCTCGTCGATGACCTCCTCATCCGCCTCAAGACCCAGAACTACTCTGTCTCCCCGGCTCTCTTTGCCCACTTGATCAAGATTTACGGCGAAGCCAATTTGCCACAGAAAGCCCTCCGCACCTTTTATACCATGGTGGAATTTGACTGCAGGCCTTCCGTCAAACACTTGAATCGCATTCTTCAAATCCTCGTTTCTCACCGGAACTTTCTCCGCCCGGCGTTCGACGTCTTCAAGGACGCTCACCGTCATGGGGTGATGCCCAATACTCAGTCTTACAACATCTTGATGCGTGCTTTTTGTTTGAATGGGGATCTTAGCATTGCCTACCAGCTATTCAACAAAATGTTTGAGAGAGACCTTGTTCCAGATGTGCAGTCGTATCGGATTCTGATGCAGGGGTTGTGCAGGAAGGGTCAGGTGAATACGGCGGTTGATTTTTTGGAGGACATGTTGAACAAAGGGTTCGTTCCGGATTCGTTGAGTTACACTAGTTTGTTGAATAGTTTGTGTAGAAAGAAGAAGCTTCGTGAGGCTTATAAGCTGCTTTGTAGGATGAAGGTTAAAGGGTGCAATCCTGATATTGTGCATTATAATACTGTTATTTTAGGATTTTGTAGAGAAGGGCGTCCAGTTGATGCTTGTAAAGTTCTTGAGGATATGGCATCAAATGGGTGCTTGCCAAATTTGGTGTCCTATCGGACTTTGGTTAGCGGGTTATGTGATCATGGGATGCTTGATGAGGCAAAGAGTTATATGGAGACAATGATATCAAGGGGGTTTTCTCCGCATTTTTCGGTTGTTCATGCTTTAGTTAAAGGATTCTGTAATGTGGGCAGGGTTGAGGAAGCCTTTGCAGTTTTGGAGGAGGTGCTAAAGCATGGGGAGGTTCCTCATACAGATACTTGGCTTACAATAGTTCCTGGGATATGCGAAGAGATTGAGCTAGAGAGATTGGAGGAAATTCTGAGAGAGGTAATGAAGGTGGAAATTAGGCCGAACACCAGAATAGTAGAGGCAGCTATTGGTTTGGAGGATTACTTGATAAAAAAGATACGGGCTAAACCGAGGAGAGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

464

Amino Acids

52.62

Weight (kDa)

9.14

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01400
fragaria_vesca FvH4_7g17740 FvH4_7g17740 FvH4_7g17740
malus_domestica MD01G1088700.v1.1
prunus_persica Prupe.2G196300_v2.0.a1
pyrus_communis pycom01g11310
rosa_chinensis RchiOBHm_Chr1g0361141
rosa_laevigata RLG00000027746
rosa_multiflora Rmu_sc0002863.1_g000035 Rmu_sc0020096.1_g000003
rosa_roxburghii Rroxscaffold_4G00295390
rosa_rugosa Rorug01G0286000 Rorug02G0035000
rosa_samantha Rh1AG297500 Rh1BG261600 Rh1CG278900 Rh1DG291400
rosa_wichuraiana Rw1G026270 Rw1G026900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 849, 903
AatII GACGTC 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 297
AccIII TCCGGA 1 cut(s) 781
AclWI GGATC 3 cut(s) 163, 176, 630
AcoI YGGCCR 2 cut(s) 228, 292
AcsI RAATTY 3 cut(s) 449, 994, 1272
AcyI GRCGYC 2 cut(s) 537, 937
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 165, 562
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 7 cut(s) 481, 494, 544, 616, 649, 766, 811
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 2 cut(s) 164, 1231
AjuI GAANNNNNNNTTGG 4 cut(s) 203, 231, 235, 263
AluBI AGCT 7 cut(s) 289, 643, 835, 853, 1255, 1331, 1385
AluI AGCT 7 cut(s) 289, 643, 835, 853, 1255, 1331, 1385
Alw26I GTCTC 3 cut(s) 364, 660, 1064
AlwI GGATC 3 cut(s) 163, 176, 630
Aor13HI TCCGGA 1 cut(s) 781
AoxI GGCC 4 cut(s) 228, 292, 461, 1304
ApeKI GCWGC 2 cut(s) 853, 1328
ApoI RAATTY 3 cut(s) 449, 994, 1272
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 2 cut(s) 365, 527
AsuHPI GGTGA 4 cut(s) 500, 545, 577, 742
AvaII GGWCC 1 cut(s) 21
BamHI GGATCC 1 cut(s) 168
BbsI GAAGAC 2 cut(s) 94, 532
BbvI GCAGC 2 cut(s) 840, 1340
BccI CCATC 1 cut(s) 85
BceAI ACGGC 2 cut(s) 412, 753
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 2 cut(s) 166, 1233
BclI TGATCA 2 cut(s) 384, 1033
BcnI CCSGG 2 cut(s) 365, 527
BcoDI GTCTC 3 cut(s) 364, 660, 1064
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 3 cut(s) 56, 801, 1256
BfmI CTRYAG 1 cut(s) 457
BglII AGATCT 1 cut(s) 216
BisI GCNGC 5 cut(s) 33, 231, 295, 854, 1329
BlsI GCNGC 5 cut(s) 34, 232, 296, 855, 1330
BmcAI AGTACT 1 cut(s) 305
Bme1390I CCNGG 4 cut(s) 166, 365, 527, 1233
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 3 cut(s) 157, 170, 1200
BmrFI CCNGG 4 cut(s) 166, 365, 527, 1233
BmsI GCATC 7 cut(s) 13, 558, 588, 693, 937, 983, 1032
BpiI GAAGAC 2 cut(s) 94, 532
BpuEI CTTGAG 3 cut(s) 269, 323, 983
BpuMI CCSGG 2 cut(s) 365, 527
BsaHI GRCGYC 2 cut(s) 537, 937
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 4 cut(s) 363, 441, 1232, 1376
BsaWI WCCGGW 2 cut(s) 510, 781
BsaXI ACNNNNNCTCC 4 cut(s) 344, 374, 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 562
Bse1I ACTGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 630
BseAI TCCGGA 1 cut(s) 781
BseBI CCWGG 2 cut(s) 166, 1233
BseDI CCNNGG 4 cut(s) 363, 441, 1232, 1376
BseGI GGATG 5 cut(s) 77, 279, 330, 870, 1047
BseLI CCNNNNNNNGG 2 cut(s) 165, 562
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 593, 1268
BseNI ACTGG 1 cut(s) 941
BseRI GAGGAG 8 cut(s) 73, 76, 79, 82, 85, 194, 317, 1190
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 2 cut(s) 840, 1340
BsgI GTGCAG 2 cut(s) 704, 736
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 4 cut(s) 230, 294, 463, 1306
BsiEI CGRYCG 1 cut(s) 295
BsiSI CCGG 4 cut(s) 365, 511, 527, 782
BslI CCNNNNNNNGG 2 cut(s) 165, 562
BsmAI GTCTC 3 cut(s) 364, 660, 1064
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 4 cut(s) 230, 294, 463, 1306
Bso31I GGTCTC 1 cut(s) 660
Bsp13I TCCGGA 1 cut(s) 781
Bsp143I GATC 5 cut(s) 168, 216, 384, 622, 1033
Bsp19I CCATGG 1 cut(s) 441
BspANI GGCC 4 cut(s) 230, 294, 463, 1306
BspCNI CTCAG 2 cut(s) 592, 1269
BspEI TCCGGA 1 cut(s) 781
BspLI GGNNCC 3 cut(s) 157, 170, 1200
BspMAI CTGCAG 1 cut(s) 461
BspPI GGATC 3 cut(s) 163, 176, 630
BspTNI GGTCTC 1 cut(s) 660
BsrBI CCGCTC 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 1 cut(s) 941
BssECI CCNNGG 4 cut(s) 363, 441, 1232, 1376
BssMI GATC 5 cut(s) 168, 216, 384, 622, 1033
BssNI GRCGYC 2 cut(s) 537, 937
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 166, 1233
Bst4CI ACNGT 3 cut(s) 43, 557, 910
Bst6I CTCTTC 2 cut(s) 268, 1239
BstACI GRCGYC 2 cut(s) 537, 937
BstC8I GCNNGC 4 cut(s) 235, 461, 603, 989
BstDEI CTNAG 3 cut(s) 579, 626, 1277
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 5 cut(s) 77, 279, 330, 870, 1047
BstKTI GATC 5 cut(s) 171, 219, 387, 625, 1036
BstMAI GTCTC 3 cut(s) 364, 660, 1064
BstMBI GATC 5 cut(s) 168, 216, 384, 622, 1033
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 3 cut(s) 16, 153, 374
BstNI CCWGG 2 cut(s) 166, 1233
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 4 cut(s) 164, 363, 525, 1231
BstSFI CTRYAG 1 cut(s) 457
BstV1I GCAGC 2 cut(s) 840, 1340
BstV2I GAAGAC 2 cut(s) 94, 532
BstX2I RGATCY 3 cut(s) 168, 216, 622
BstYI RGATCY 3 cut(s) 168, 216, 622
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 4 cut(s) 230, 294, 463, 1306
BtgI CCRYGG 1 cut(s) 441
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 5 cut(s) 77, 279, 330, 870, 1047
Cac8I GCNNGC 4 cut(s) 235, 461, 603, 989
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 2 cut(s) 557, 926
Csp6I GTAC 1 cut(s) 304
CviAII CATG 6 cut(s) 442, 560, 760, 1037, 1115, 1190
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 3 cut(s) 579, 626, 1277
DpnI GATC 5 cut(s) 170, 218, 386, 624, 1035
DpnII GATC 5 cut(s) 168, 216, 384, 622, 1033
EaeI YGGCCR 2 cut(s) 228, 292
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 268, 1239
EarI CTCTTC 2 cut(s) 268, 1239
EciI GGCGGA 2 cut(s) 323, 512
EclXI CGGCCG 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 441
Eco147I AGGCCT 1 cut(s) 463
Eco31I GGTCTC 1 cut(s) 660
Eco32I GATATC 1 cut(s) 1080
Eco47I GGWCC 1 cut(s) 21
Eco52I CGGCCG 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 164, 1231
EcoRV GATATC 1 cut(s) 1080
EcoT14I CCWWGG 1 cut(s) 441
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 6 cut(s) 445, 563, 763, 1040, 1118, 1193
FatI CATG 6 cut(s) 441, 559, 759, 1036, 1114, 1189
FauI CCCGC 3 cut(s) 72, 242, 1016
FbaI TGATCA 2 cut(s) 384, 1033
Fnu4HI GCNGC 5 cut(s) 33, 231, 295, 854, 1329
FokI GGATG 5 cut(s) 64, 266, 317, 877, 1054
Fsp4HI GCNGC 5 cut(s) 33, 231, 295, 854, 1329
FspBI CTAG 3 cut(s) 56, 801, 1256
GluI GCNGC 5 cut(s) 33, 231, 295, 854, 1329
HaeIII GGCC 4 cut(s) 230, 294, 463, 1306
HapII CCGG 4 cut(s) 365, 511, 527, 782
HgaI GACGC 2 cut(s) 557, 926
Hin1I GRCGYC 2 cut(s) 537, 937
Hin1II CATG 6 cut(s) 445, 563, 763, 1040, 1118, 1193
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 4 cut(s) 481, 697, 785, 1131
HpaII CCGG 4 cut(s) 365, 511, 527, 782
HphI GGTGA 4 cut(s) 500, 545, 577, 742
Hpy188I TCNGA 5 cut(s) 196, 696, 702, 1011, 1278
Hpy188III TCNNGA 8 cut(s) 340, 388, 595, 677, 782, 839, 887, 962
Hpy99I CGWCG 2 cut(s) 320, 539
HpyAV CCTTC 7 cut(s) 171, 277, 474, 715, 862, 926, 1285
HpyCH4III ACNGT 3 cut(s) 43, 557, 910
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 8 cut(s) 4, 459, 685, 706, 717, 882, 898, 1166
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 153, 374
HpyF3I CTNAG 3 cut(s) 579, 626, 1277
HpySE526I ACGT 1 cut(s) 537
Hsp92I GRCGYC 2 cut(s) 537, 937
Hsp92II CATG 6 cut(s) 445, 563, 763, 1040, 1118, 1193
Kpn2I TCCGGA 1 cut(s) 781
Ksp22I TGATCA 2 cut(s) 384, 1033
Kzo9I GATC 5 cut(s) 168, 216, 384, 622, 1033
LmnI GCTCC 2 cut(s) 161, 302
Lsp1109I GCAGC 2 cut(s) 840, 1340
LweI GCATC 7 cut(s) 13, 558, 588, 693, 937, 983, 1032
MaeI CTAG 3 cut(s) 56, 801, 1256
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 3 cut(s) 37, 43, 794
MalI GATC 5 cut(s) 170, 218, 386, 624, 1035
MbiI CCGCTC 1 cut(s) 297
MboI GATC 5 cut(s) 168, 216, 384, 622, 1033
MboII GAAGA 9 cut(s) 94, 97, 211, 255, 262, 482, 532, 841, 1256
MflI RGATCY 3 cut(s) 168, 216, 622
MluCI AATT 5 cut(s) 406, 449, 994, 1272, 1299
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 781
MseI TTAA 2 cut(s) 873, 1125
MslI CAYNNNNRTG 2 cut(s) 558, 596
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 4 cut(s) 365, 511, 527, 782
MspR9I CCNGG 4 cut(s) 166, 365, 527, 1233
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 2 cut(s) 166, 1233
MwoI GCNNNNNNNGC 3 cut(s) 16, 153, 374
NciI CCSGG 2 cut(s) 365, 527
NcoI CCATGG 1 cut(s) 441
NdeII GATC 5 cut(s) 168, 216, 384, 622, 1033
NlaIII CATG 6 cut(s) 445, 563, 763, 1040, 1118, 1193
NlaIV GGNNCC 3 cut(s) 157, 170, 1200
NmeAIII GCCGAG 1 cut(s) 270
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 763
PceI AGGCCT 1 cut(s) 463
PciI ACATGT 1 cut(s) 759
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 4 cut(s) 481, 697, 785, 1131
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 5 cut(s) 34, 232, 296, 855, 1330
PscI ACATGT 1 cut(s) 759
PsiI TTATAA 2 cut(s) 849, 903
Psp6I CCWGG 2 cut(s) 164, 1231
PspGI CCWGG 2 cut(s) 164, 1231
PspN4I GGNNCC 3 cut(s) 157, 170, 1200
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 461
PsuI RGATCY 3 cut(s) 168, 216, 622
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 558, 596
SaqAI TTAA 2 cut(s) 873, 1125
SatI GCNGC 5 cut(s) 33, 231, 295, 854, 1329
Sau3AI GATC 5 cut(s) 168, 216, 384, 622, 1033
Sau96I GGNCC 1 cut(s) 21
ScaI AGTACT 1 cut(s) 305
ScrFI CCNGG 4 cut(s) 166, 365, 527, 1233
SfaNI GCATC 7 cut(s) 13, 558, 588, 693, 937, 983, 1032
SfcI CTRYAG 1 cut(s) 457
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 2 cut(s) 558, 596
SmlI CTYRAG 3 cut(s) 284, 338, 962
SmoI CTYRAG 3 cut(s) 284, 338, 962
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 5 cut(s) 406, 449, 994, 1272, 1299
SseBI AGGCCT 1 cut(s) 463
SspMI CTAG 3 cut(s) 56, 801, 1256
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 4 cut(s) 164, 363, 525, 1231
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 3 cut(s) 43, 557, 910
TaiI ACGT 1 cut(s) 540
TaqI TCGA 3 cut(s) 222, 318, 534
TasI AATT 5 cut(s) 406, 449, 994, 1272, 1299
TatI WGTACW 1 cut(s) 303
TauI GCSGC 3 cut(s) 35, 233, 297
TfiI GAWTC 4 cut(s) 481, 697, 785, 1131
Tru1I TTAA 2 cut(s) 873, 1125
Tru9I TTAA 2 cut(s) 873, 1125
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 853, 1328
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 3 cut(s) 881, 1103, 1304
TspGWI ACGGA 2 cut(s) 88, 457
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 3 cut(s) 449, 994, 1272
XceI RCATGY 1 cut(s) 763
XspI CTAG 3 cut(s) 56, 801, 1256
ZraI GACGTC 1 cut(s) 538
ZrmI AGTACT 1 cut(s) 305
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.