Prupe.2G325700_v2.0.a1

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0015697
Physical Location & Seq
Reverse (-)
30082554 .. 30085325
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G325700.1

Sequence Viewer

Length: 1233 bp
ATGGCTGGGTCCACCTCGACCGGCGGGAGCGGCCCGTCGAATCCTCCCAAAATCCTACTAGCAAAGCCGGGACTGGTGACGGGGGCTCCGGTGGCGGGTAAATTCGGGCGCGCCGGTACTGGGGATGAGTATACGACGTCGCTTCGGTCCCGTCTCCCTTCTTCTATCGGATCCCTCAACCTCCTCTCTGATTCATGGGACTTCCACATAGACCGCTTCCTCCCGTTTTTGACTGAGAACACGGAGTTTACGGTGGTGGGGGTGATTGGTCCGCCTGGGGTGGGCAAGTCCACGATTATGAACGAGCTTTATGGCTTCGATGCGACTTCACCTGGAATGTTACCCCCATTTGCTATAGAGACGGAAGAGGGTAAAGCCATGGCAAGGCATTGCAGCATGGGCATTGAACCCAGGGTTTCTGCTGAACGCCTTATACTTCTTGATACCCAGCCTGTTTTTAGTCCTTCTGTTTTAGCTGAGATGATGAGACCGGATGGCTCGTCCACCGTTCCTGTTCTAACTACTGCTGAATCTTTATCTGCTGAGTTAGCTCATGAACTTATGGGTATTCAGCTTGGTGTTCTTTTGACCTCAATCTGTCACATTTTGCTGGTTATATCAGAGGGAGTTCATGATCATAGTACGTGGCGTTTGATGTCCACGGTTGACCTTTTGAAACATGGCATACCTGATCCATCTTCACCAACCCTTTCCAATTCTCTGAGCTCTAATATAGGGCCTGAAAAAGATAGCAGAGATAAAGCTCATGAAGGTGGAGAATATATGGCTACTCCAGTTTTTGTGCACACAAAACTACGGGACCATGATCTTAGTCCACATAATTTTGTGCAACTGAGGAAGGCACTTGCACAATATCTCAGCACCTCCTCTTTCATGAGACCAGAAAATGGAAATATGTCCAAAAGTCGTGATCCAGATTCTATGGCTCCTGGTACTCAAAGTCGCGATCCAGATTCTTTAGTGCCAAGTTTGTTTGTAATCCCAACAAAGAATAAAGATGACTCCCCAGGAGATCAGTATGAAAGTTATACCTCTATGCTATGGAAATTGCGGGATAAGGTTTTGTCCATGAAATCTCCTCCTTTTGGAAGAACTGTGAGTGAACGTGAATGGTTGAAGAATTCTGCCAAGATATGGGAATTGGTGAAGAACTCTCCTATCATTGCAGAATACAGCAGAACACTTCAAAGTTGTGGAATGTTTAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

44.62

Weight (kDa)

6.14

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33920 FvH4_7g33920
malus_domestica MD07G1308500.v1.1
prunus_persica Prupe.2G325700_v2.0.a1
pyrus_communis pycom07g28070
rosa_chinensis RchiOBHm_Chr1g0384131
rosa_laevigata RLG00000026069
rosa_multiflora Rmu_sc0005139.1_g000016 Rmu_sc0014419.1_g000001
rosa_roxburghii Rroxscaffold_4G00276960
rosa_rugosa Rorug01G0445100
rosa_samantha Rh1AG470600 Rh1BG428000 Rh1CG440700 Rh1DG461000
rosa_wichuraiana Rw1G040210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 140
AccB7I CCANNNNNTGG 2 cut(s) 908, 1155
AccBSI CCGCTC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 131
AccII CGCG 2 cut(s) 111, 966
AciI CCGC 6 cut(s) 24, 30, 95, 214, 272, 1072
AclWI GGATC 5 cut(s) 165, 178, 686, 926, 962
AcsI RAATTY 2 cut(s) 101, 1141
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 3 cut(s) 118, 643, 955
AfiI CCNNNNNNNGG 7 cut(s) 95, 120, 281, 384, 908, 1106, 1155
AgsI TTSAA 4 cut(s) 407, 676, 1138, 1208
AjnI CCWGG 5 cut(s) 274, 331, 410, 949, 1027
AluBI AGCT 6 cut(s) 307, 476, 551, 574, 726, 764
AluI AGCT 6 cut(s) 307, 476, 551, 574, 726, 764
Alw21I GWGCWC 2 cut(s) 728, 807
Alw26I GTCTC 4 cut(s) 158, 353, 481, 892
Alw44I GTGCAC 1 cut(s) 803
AlwI GGATC 5 cut(s) 165, 178, 686, 926, 962
AoxI GGCC 2 cut(s) 31, 737
ApaLI GTGCAC 1 cut(s) 803
ApeKI GCWGC 1 cut(s) 393
ApoI RAATTY 2 cut(s) 101, 1141
AscI GGCGCGCC 1 cut(s) 109
AspLEI GCGC 2 cut(s) 111, 113
AspS9I GGNCC 6 cut(s) 9, 32, 147, 269, 737, 820
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 5 cut(s) 88, 274, 321, 693, 1177
AvaII GGWCC 4 cut(s) 9, 147, 269, 820
BaeGI GKGCMC 1 cut(s) 807
BamHI GGATCC 1 cut(s) 170
BanII GRGCYC 2 cut(s) 88, 728
Bbv12I GWGCWC 2 cut(s) 728, 807
BbvI GCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 488, 703
BciT130I CCWGG 5 cut(s) 276, 333, 412, 951, 1029
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 4 cut(s) 158, 353, 481, 892
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 354
BisI GCNGC 2 cut(s) 31, 394
BlsI GCNGC 2 cut(s) 32, 395
Bme1390I CCNGG 6 cut(s) 69, 276, 333, 412, 951, 1029
Bme18I GGWCC 4 cut(s) 9, 147, 269, 820
BmgT120I GGNCC 6 cut(s) 9, 32, 147, 269, 737, 820
BmiI GGNNCC 6 cut(s) 10, 87, 149, 172, 821, 948
BmrFI CCNGG 6 cut(s) 69, 276, 333, 412, 951, 1029
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 481, 892
BsaJI CCNNGG 6 cut(s) 275, 378, 410, 411, 660, 1027
BsaWI WCCGGW 2 cut(s) 88, 490
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 120, 281, 384, 908, 1106, 1155
Bse118I RCCGGY 2 cut(s) 20, 113
Bse1I ACTGG 3 cut(s) 78, 124, 794
Bse3DI GCAATG 2 cut(s) 388, 1182
BseBI CCWGG 5 cut(s) 276, 333, 412, 951, 1029
BseDI CCNNGG 6 cut(s) 275, 378, 410, 411, 660, 1027
BseGI GGATG 2 cut(s) 130, 499
BseLI CCNNNNNNNGG 7 cut(s) 95, 120, 281, 384, 908, 1106, 1155
BseMI GCAATG 2 cut(s) 388, 1182
BseMII CTCAG 6 cut(s) 225, 468, 534, 713, 845, 892
BseNI ACTGG 3 cut(s) 78, 124, 794
BsePI GCGCGC 1 cut(s) 109
BseRI GAGGAG 3 cut(s) 173, 877, 1089
BseSI GKGCMC 1 cut(s) 807
BseXI GCAGC 1 cut(s) 405
BseYI CCCAGC 2 cut(s) 5, 447
Bsh1236I CGCG 2 cut(s) 111, 966
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 33, 739
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 728, 807
BsiSI CCGG 5 cut(s) 21, 68, 89, 114, 491
BslFI GGGAC 4 cut(s) 84, 133, 212, 833
BslI CCNNNNNNNGG 7 cut(s) 95, 120, 281, 384, 908, 1106, 1155
BsmAI GTCTC 4 cut(s) 158, 353, 481, 892
BsmBI CGTCTC 2 cut(s) 158, 353
BsmFI GGGAC 4 cut(s) 84, 133, 212, 833
BsnI GGCC 2 cut(s) 33, 739
Bso31I GGTCTC 2 cut(s) 481, 892
Bsp1286I GDGCHC 3 cut(s) 88, 728, 807
Bsp143I GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
Bsp19I CCATGG 1 cut(s) 378
Bsp68I TCGCGA 1 cut(s) 966
BspACI CCGC 6 cut(s) 24, 30, 95, 214, 272, 1072
BspANI GGCC 2 cut(s) 33, 739
BspCNI CTCAG 6 cut(s) 226, 469, 535, 714, 846, 891
BspFNI CGCG 2 cut(s) 111, 966
BspHI TCATGA 4 cut(s) 553, 631, 766, 894
BspLI GGNNCC 6 cut(s) 10, 87, 149, 172, 821, 948
BspPI GGATC 5 cut(s) 165, 178, 686, 926, 962
BspTNI GGTCTC 2 cut(s) 481, 892
BsrBI CCGCTC 1 cut(s) 30
BsrDI GCAATG 2 cut(s) 388, 1182
BsrFI RCCGGY 2 cut(s) 20, 113
BsrI ACTGG 3 cut(s) 78, 124, 794
BssAI RCCGGY 2 cut(s) 20, 113
BssECI CCNNGG 6 cut(s) 275, 378, 410, 411, 660, 1027
BssHII GCGCGC 1 cut(s) 109
BssMI GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
BssNAI GTATAC 1 cut(s) 132
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 378
Bst1107I GTATAC 1 cut(s) 132
Bst2UI CCWGG 5 cut(s) 276, 333, 412, 951, 1029
Bst4CI ACNGT 4 cut(s) 253, 508, 664, 1117
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 7 cut(s) 234, 477, 543, 722, 830, 854, 878
BstDSI CCRYGG 2 cut(s) 378, 660
BstF5I GGATG 2 cut(s) 130, 499
BstFNI CGCG 2 cut(s) 111, 966
BstHHI GCGC 2 cut(s) 111, 113
BstKTI GATC 7 cut(s) 173, 637, 694, 829, 934, 970, 1036
BstMAI GTCTC 4 cut(s) 158, 353, 481, 892
BstMBI GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 4 cut(s) 30, 92, 399, 548
BstNI CCWGG 5 cut(s) 276, 333, 412, 951, 1029
BstSCI CCNGG 6 cut(s) 67, 274, 331, 410, 949, 1027
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 807
BstUI CGCG 2 cut(s) 111, 966
BstV1I GCAGC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BstZ17I GTATAC 1 cut(s) 132
BsuRI GGCC 2 cut(s) 33, 739
BtgI CCRYGG 2 cut(s) 378, 660
BtsCI GGATG 2 cut(s) 130, 499
BtuMI TCGCGA 1 cut(s) 966
Cac8I GCNNGC 1 cut(s) 111
CciI TCATGA 4 cut(s) 553, 631, 766, 894
CfoI GCGC 2 cut(s) 111, 113
Cfr10I RCCGGY 2 cut(s) 20, 113
Cfr13I GGNCC 6 cut(s) 9, 32, 147, 269, 737, 820
Csp6I GTAC 3 cut(s) 117, 642, 954
CviQI GTAC 3 cut(s) 117, 642, 954
DdeI CTNAG 7 cut(s) 234, 477, 543, 722, 830, 854, 878
DpnI GATC 7 cut(s) 172, 636, 693, 828, 933, 969, 1035
DpnII GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 726
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 2 cut(s) 88, 728
Eco31I GGTCTC 2 cut(s) 481, 892
Eco47I GGWCC 4 cut(s) 9, 147, 269, 820
Eco53kI GAGCTC 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 1141
EcoRII CCWGG 5 cut(s) 274, 331, 410, 949, 1027
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 2 cut(s) 88, 728
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 2 cut(s) 158, 353
FaqI GGGAC 4 cut(s) 84, 133, 212, 833
FauI CCCGC 3 cut(s) 17, 88, 1065
FbaI TGATCA 1 cut(s) 634
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 2 cut(s) 31, 394
FokI GGATG 2 cut(s) 137, 506
FriOI GRGCYC 2 cut(s) 88, 728
Fsp4HI GCNGC 2 cut(s) 31, 394
FspBI CTAG 1 cut(s) 59
GlaI GCGC 2 cut(s) 110, 112
GluI GCNGC 2 cut(s) 31, 394
GsaI CCCAGC 2 cut(s) 9, 451
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 2 cut(s) 33, 739
HapII CCGG 5 cut(s) 21, 68, 89, 114, 491
HhaI GCGC 2 cut(s) 111, 113
Hin1I GRCGYC 1 cut(s) 137
Hin6I GCGC 2 cut(s) 109, 111
HinP1I GCGC 2 cut(s) 109, 111
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 6 cut(s) 40, 191, 530, 938, 974, 1022
HpaII CCGG 5 cut(s) 21, 68, 89, 114, 491
HphI GGTGA 5 cut(s) 88, 274, 321, 693, 1177
Hpy188I TCNGA 4 cut(s) 170, 190, 622, 723
Hpy188III TCNNGA 9 cut(s) 440, 554, 632, 767, 895, 929, 935, 965, 971
Hpy99I CGWCG 3 cut(s) 40, 139, 142
HpyAV CCTTC 4 cut(s) 168, 474, 764, 853
HpyCH4III ACNGT 4 cut(s) 253, 508, 664, 1117
HpyCH4IV ACGT 3 cut(s) 137, 644, 1126
HpyCH4V TGCA 5 cut(s) 393, 805, 850, 869, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 92, 399, 548
HpyF3I CTNAG 7 cut(s) 234, 477, 543, 722, 830, 854, 878
HpySE526I ACGT 3 cut(s) 137, 644, 1126
Hsp92I GRCGYC 1 cut(s) 137
HspAI GCGC 2 cut(s) 109, 111
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
LmnI GCTCC 3 cut(s) 27, 91, 952
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 1 cut(s) 310
MaeI CTAG 1 cut(s) 59
MaeII ACGT 3 cut(s) 137, 644, 1126
MaeIII GTNAC 3 cut(s) 76, 339, 599
MalI GATC 7 cut(s) 172, 636, 693, 828, 933, 969, 1035
MbiI CCGCTC 1 cut(s) 30
MboI GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
MboII GAAGA 6 cut(s) 153, 377, 690, 1122, 1150, 1180
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 3 cut(s) 88, 728, 807
MluCI AATT 6 cut(s) 101, 715, 841, 1067, 1141, 1160
MlyI GAGTC 1 cut(s) 1016
MslI CAYNNNNRTG 2 cut(s) 296, 771
MspI CCGG 5 cut(s) 21, 68, 89, 114, 491
MspR9I CCNGG 6 cut(s) 69, 276, 333, 412, 951, 1029
MvaI CCWGG 5 cut(s) 276, 333, 412, 951, 1029
MvnI CGCG 2 cut(s) 111, 966
MwoI GCNNNNNNNGC 4 cut(s) 30, 92, 399, 548
NciI CCSGG 1 cut(s) 69
NcoI CCATGG 1 cut(s) 378
NdeII GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
NlaIV GGNNCC 6 cut(s) 10, 87, 149, 172, 821, 948
NmuCI GTSAC 2 cut(s) 76, 599
NruI TCGCGA 1 cut(s) 966
PagI TCATGA 4 cut(s) 553, 631, 766, 894
PalAI GGCGCGCC 1 cut(s) 109
PasI CCCWGGG 1 cut(s) 411
PauI GCGCGC 1 cut(s) 109
PfeI GAWTC 5 cut(s) 40, 191, 530, 938, 974
PflMI CCANNNNNTGG 2 cut(s) 908, 1155
PkrI GCNGC 2 cut(s) 32, 395
PleI GAGTC 1 cut(s) 1016
PpsI GAGTC 1 cut(s) 1016
Ppu21I YACGTR 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 728
Psp6I CCWGG 5 cut(s) 274, 331, 410, 949, 1027
PspFI CCCAGC 2 cut(s) 5, 447
PspGI CCWGG 5 cut(s) 274, 331, 410, 949, 1027
PspN4I GGNNCC 6 cut(s) 10, 87, 149, 172, 821, 948
PspPI GGNCC 6 cut(s) 9, 32, 147, 269, 737, 820
PsrI GAACNNNNNNTAC 2 cut(s) 417, 449
PsuI RGATCY 1 cut(s) 170
PteI GCGCGC 1 cut(s) 109
RruI TCGCGA 1 cut(s) 966
RsaI GTAC 3 cut(s) 118, 643, 955
RsaNI GTAC 3 cut(s) 117, 642, 954
RseI CAYNNNNRTG 2 cut(s) 296, 771
SacI GAGCTC 1 cut(s) 728
SatI GCNGC 2 cut(s) 31, 394
Sau3AI GATC 7 cut(s) 170, 634, 691, 826, 931, 967, 1033
Sau96I GGNCC 6 cut(s) 9, 32, 147, 269, 737, 820
SchI GAGTC 1 cut(s) 1016
ScrFI CCNGG 6 cut(s) 69, 276, 333, 412, 951, 1029
SduI GDGCHC 3 cut(s) 88, 728, 807
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 354
SgsI GGCGCGCC 1 cut(s) 109
SinI GGWCC 4 cut(s) 9, 147, 269, 820
SmiMI CAYNNNNRTG 2 cut(s) 296, 771
Sse9I AATT 6 cut(s) 101, 715, 841, 1067, 1141, 1160
SsiI CCGC 6 cut(s) 24, 30, 95, 214, 272, 1072
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 728
StyD4I CCNGG 6 cut(s) 67, 274, 331, 410, 949, 1027
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 253, 508, 664, 1117
TaiI ACGT 3 cut(s) 140, 647, 1129
TaqI TCGA 3 cut(s) 17, 38, 318
TaqII GACCGA 1 cut(s) 135
TasI AATT 6 cut(s) 101, 715, 841, 1067, 1141, 1160
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 5 cut(s) 40, 191, 530, 938, 974
TseFI GTSAC 2 cut(s) 76, 599
TseI GCWGC 1 cut(s) 393
Tsp45I GTSAC 2 cut(s) 76, 599
TspDTI ATGAA 8 cut(s) 183, 314, 570, 620, 783, 883, 1056, 1106
TspGWI ACGGA 2 cut(s) 257, 377
Van91I CCANNNNNTGG 2 cut(s) 908, 1155
VneI GTGCAC 1 cut(s) 803
VpaK11BI GGWCC 4 cut(s) 9, 147, 269, 820
XapI RAATTY 2 cut(s) 101, 1141
XmiI GTMKAC 1 cut(s) 131
XspI CTAG 1 cut(s) 59
ZraI GACGTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.