pycom07g28070

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015697
Physical Location & Seq
Reverse (-)
27543888 .. 27546342
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28070.1

Sequence Viewer

Length: 1281 bp
ATGAGGCCCGGCAACCCAAACGACGGTGTAAACTCCTTTATCATATCGATGGCTGGGTCCGCCACCTCGACCGGCGGGAGCGGTCCGTCGAATCCTCCGAAAATCCTACTAGCAAAGCCGGGACTCGTGCCGGGGACTCCGGTGGCGGGTAAATTCGGGCGTGGCGGCACCGGCGACGAGTACACGACGTCTCTTCGGTCCCGTCTCCCTTCTTCTATCGGATCCCTCAACCTCCTCTCTGATTCCTGGGACTTCCACATCGACCGCTTCCTTCCGTTTTTGACTGAGAACACTGAGTTTACGGTGGTGGGGGTGATCGGTCCAACGGGGGTCGGCAAGTCCACGATTATGAACGAGCTTTACGGCTTCGATGCCACCTCACCTGGAATGTTACCCCCATTTGCTATAGAGACGGAAGAGGGTAAAGCAATGGCAAGGCATTGCAGCATGGGCATTGAACCCCGGGTTTCTGCTGAACGCATTATCCTTCTCGATACTCAGCCTGTTTTTAGTCCTTCTGTTTTAGCTGAGATGATGAGACCCGACGGCTCATCAACCATTCCTGTTCTACCTTCAGCCGAGTCTTTATCTGCTGAGTTAGCTCATGAGCTTATAGGTATTCAGCTTGGTGTTCTTCTGACCTCCATCTGTCACTTTTTGCTGGTTATATCAGACGGAGTTCATGATCATAAAATGTGGCGTCTAATGTCCACGGTCGACCTCTTGAAACATGGCATTCCTGATCCATCATCACTGACCCTTGCCCCTTCCCAAAGCTCTAATACAGGGCCTGAAAAGGATAGAGAGAAAGTTCATGAAGGCAGAGAATATATGGCTATGCCCGTCTTTGTGCACACAAAACTGCGTGATCGTGATCTTAGTCCACATAATATTGCGCGATTGAGGAAGGCACTTGCACAATATTTCAGTACCTCTTCTCTCACGAAGGCAGACAATGGAAACACGTCCATAAGTCGTGATCCAGATTCTGTGGCTCCTGGTACTCAAAGTCATGATCTGGATTCCTTAATGCCAAGTTTGTTTGTAATCCCAACAAAGAATAAAGATGACTCCCCAGGAGATCAGTATGAAAGTTATACCTCTATGCTATGGAAATTGCGAGATAAGGTTTTATCAATGAATTCTCCTCCATTTGGAAGGACGGTGACGGAACGTGACTGGTTGAAGAATTCTGCAAAGATATGGGAACTGGTGAAGAGCTCTCCGATCATTGCAGAATACAGCAGAACACTTCAAAGTTGTGGAATGTTTAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

46.34

Weight (kDa)

6.27

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33920 FvH4_7g33920
malus_domestica MD07G1308500.v1.1
prunus_persica Prupe.2G325700_v2.0.a1
pyrus_communis pycom07g28070
rosa_chinensis RchiOBHm_Chr1g0384131
rosa_laevigata RLG00000026069
rosa_multiflora Rmu_sc0005139.1_g000016 Rmu_sc0014419.1_g000001
rosa_roxburghii Rroxscaffold_4G00276960
rosa_rugosa Rorug01G0445100
rosa_samantha Rh1AG470600 Rh1BG428000 Rh1CG440700 Rh1DG461000
rosa_wichuraiana Rw1G040210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 717
AccII CGCG 1 cut(s) 898
AciI CCGC 6 cut(s) 60, 75, 81, 146, 165, 265
AclWI GGATC 4 cut(s) 216, 229, 737, 974
AcsI RAATTY 3 cut(s) 152, 1141, 1189
AcuI CTGAAG 1 cut(s) 558
AcyI GRCGYC 2 cut(s) 188, 700
AfaI GTAC 3 cut(s) 182, 931, 1003
AfiI CCNNNNNNNGG 3 cut(s) 23, 146, 1154
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 4 cut(s) 458, 727, 1186, 1256
AjiI CACGTC 1 cut(s) 966
AjnI CCWGG 4 cut(s) 245, 382, 997, 1075
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 7 cut(s) 358, 527, 602, 610, 625, 777, 1221
AluI AGCT 7 cut(s) 358, 527, 602, 610, 625, 777, 1221
Alw21I GWGCWC 2 cut(s) 855, 1223
Alw26I GTCTC 4 cut(s) 195, 209, 404, 532
Alw44I GTGCAC 1 cut(s) 851
AlwI GGATC 4 cut(s) 216, 229, 737, 974
AlwNI CAGNNNCTG 2 cut(s) 791, 989
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 2 cut(s) 5, 788
ApaLI GTGCAC 1 cut(s) 851
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 3 cut(s) 152, 1141, 1189
AspLEI GCGC 1 cut(s) 898
AspS9I GGNCC 6 cut(s) 6, 57, 83, 198, 320, 788
AsuC2I CCSGG 5 cut(s) 9, 120, 132, 463, 464
AsuHPI GGTGA 4 cut(s) 325, 372, 1177, 1225
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 4 cut(s) 57, 83, 198, 320
BaeGI GKGCMC 1 cut(s) 855
BamHI GGATCC 1 cut(s) 221
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 1223
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 855, 1223
BbvI GCAGC 1 cut(s) 456
BccI CCATC 3 cut(s) 43, 653, 754
BceAI ACGGC 2 cut(s) 379, 562
BciT130I CCWGG 4 cut(s) 247, 384, 999, 1077
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 5 cut(s) 9, 120, 132, 463, 464
BcoDI GTCTC 4 cut(s) 195, 209, 404, 532
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 405
BisI GCNGC 2 cut(s) 166, 445
BlsI GCNGC 2 cut(s) 167, 446
Bme1390I CCNGG 9 cut(s) 9, 120, 132, 247, 384, 463, 464, 999, 1077
Bme18I GGWCC 4 cut(s) 57, 83, 198, 320
BmeT110I CYCGRG 1 cut(s) 462
BmgBI CACGTC 1 cut(s) 966
BmgT120I GGNCC 6 cut(s) 6, 57, 83, 198, 320, 788
BmiI GGNNCC 5 cut(s) 58, 169, 200, 223, 996
BmrFI CCNGG 9 cut(s) 9, 120, 132, 247, 384, 463, 464, 999, 1077
BmsI GCATC 1 cut(s) 361
BpuMI CCSGG 5 cut(s) 9, 120, 132, 463, 464
Bsa29I ATCGAT 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 873
BsaHI GRCGYC 2 cut(s) 188, 700
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 6 cut(s) 131, 246, 461, 462, 711, 1075
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 146, 1154
Bse118I RCCGGY 2 cut(s) 71, 170
Bse1I ACTGG 2 cut(s) 1184, 1215
Bse3DI GCAATG 3 cut(s) 435, 439, 1230
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 4 cut(s) 247, 384, 999, 1077
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 6 cut(s) 131, 246, 461, 462, 711, 1075
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 3 cut(s) 23, 146, 1154
BseMI GCAATG 3 cut(s) 435, 439, 1230
BseMII CTCAG 5 cut(s) 276, 285, 512, 519, 585
BseNI ACTGG 2 cut(s) 1184, 1215
BseRI GAGGAG 2 cut(s) 224, 1137
BseSI GKGCMC 1 cut(s) 855
BseXI GCAGC 1 cut(s) 456
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 898
Bsh1285I CGRYCG 3 cut(s) 72, 265, 717
BshFI GGCC 2 cut(s) 7, 790
BshNI GGYRCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 47
BsiEI CGRYCG 3 cut(s) 72, 265, 717
BsiHKAI GWGCWC 2 cut(s) 855, 1223
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 7 cut(s) 9, 72, 119, 131, 140, 171, 463
BslFI GGGAC 4 cut(s) 135, 148, 184, 263
BslI CCNNNNNNNGG 3 cut(s) 23, 146, 1154
BsmAI GTCTC 4 cut(s) 195, 209, 404, 532
BsmBI CGTCTC 3 cut(s) 195, 209, 404
BsmFI GGGAC 4 cut(s) 135, 148, 184, 263
BsmI GAATGC 1 cut(s) 735
BsnI GGCC 2 cut(s) 7, 790
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 2 cut(s) 855, 1223
BspACI CCGC 6 cut(s) 60, 75, 81, 146, 165, 265
BspANI GGCC 2 cut(s) 7, 790
BspCNI CTCAG 5 cut(s) 277, 286, 511, 520, 586
BspDI ATCGAT 1 cut(s) 47
BspFNI CGCG 1 cut(s) 898
BspHI TCATGA 4 cut(s) 604, 682, 814, 1012
BspLI GGNNCC 5 cut(s) 58, 169, 200, 223, 996
BspPI GGATC 4 cut(s) 216, 229, 737, 974
BspQI GCTCTTC 1 cut(s) 1211
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 532
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 3 cut(s) 435, 439, 1230
BsrFI RCCGGY 2 cut(s) 71, 170
BsrI ACTGG 2 cut(s) 1184, 1215
BssAI RCCGGY 2 cut(s) 71, 170
BssECI CCNNGG 6 cut(s) 131, 246, 461, 462, 711, 1075
BssNI GRCGYC 2 cut(s) 188, 700
BssSI CACGAG 1 cut(s) 125
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 4 cut(s) 247, 384, 999, 1077
Bst4CI ACNGT 4 cut(s) 26, 304, 715, 1165
Bst6I CTCTTC 4 cut(s) 198, 411, 940, 1211
BstACI GRCGYC 2 cut(s) 188, 700
BstDEI CTNAG 6 cut(s) 285, 294, 498, 528, 594, 878
BstDSI CCRYGG 1 cut(s) 711
BstFNI CGCG 1 cut(s) 898
BstHHI GCGC 1 cut(s) 898
BstMAI GTCTC 4 cut(s) 195, 209, 404, 532
BstMCI CGRYCG 3 cut(s) 72, 265, 717
BstMWI GCNNNNNNNGC 4 cut(s) 59, 171, 450, 599
BstNI CCWGG 4 cut(s) 247, 384, 999, 1077
BstSCI CCNGG 9 cut(s) 7, 118, 130, 245, 382, 461, 462, 997, 1075
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 855
BstUI CGCG 1 cut(s) 898
BstV1I GCAGC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 2 cut(s) 7, 790
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 711
BtrI CACGTC 1 cut(s) 966
BtsIMutI CAGTG 2 cut(s) 291, 752
CaiI CAGNNNCTG 2 cut(s) 791, 989
CciI TCATGA 4 cut(s) 604, 682, 814, 1012
CfoI GCGC 1 cut(s) 898
Cfr10I RCCGGY 2 cut(s) 71, 170
Cfr13I GGNCC 6 cut(s) 6, 57, 83, 198, 320, 788
Cfr9I CCCGGG 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 47
CpoI CGGWCCG 1 cut(s) 83
CseI GACGC 1 cut(s) 689
Csp6I GTAC 3 cut(s) 181, 930, 1002
CspI CGGWCCG 1 cut(s) 83
CviAII CATG 6 cut(s) 448, 605, 683, 731, 815, 1013
CviQI GTAC 3 cut(s) 181, 930, 1002
DdeI CTNAG 6 cut(s) 285, 294, 498, 528, 594, 878
Eam1104I CTCTTC 4 cut(s) 198, 411, 940, 1211
EarI CTCTTC 4 cut(s) 198, 411, 940, 1211
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 1221
Eco24I GRGCYC 1 cut(s) 1223
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 4 cut(s) 57, 83, 198, 320
Eco53kI GAGCTC 1 cut(s) 1221
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 1221
EcoO109I RGGNCCY 1 cut(s) 788
EcoRI GAATTC 2 cut(s) 1141, 1189
EcoRII CCWGG 4 cut(s) 245, 382, 997, 1075
EcoT38I GRGCYC 1 cut(s) 1223
Esp3I CGTCTC 3 cut(s) 195, 209, 404
FaeI CATG 6 cut(s) 451, 608, 686, 734, 818, 1016
FaqI GGGAC 4 cut(s) 135, 148, 184, 263
FatI CATG 6 cut(s) 447, 604, 682, 730, 814, 1012
FauI CCCGC 2 cut(s) 68, 139
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 2 cut(s) 166, 445
FriOI GRGCYC 1 cut(s) 1223
Fsp4HI GCNGC 2 cut(s) 166, 445
FspBI CTAG 1 cut(s) 110
GlaI GCGC 1 cut(s) 897
GluI GCNGC 2 cut(s) 166, 445
GsaI CCCAGC 1 cut(s) 57
HaeIII GGCC 2 cut(s) 7, 790
HapII CCGG 7 cut(s) 9, 72, 119, 131, 140, 171, 463
HgaI GACGC 1 cut(s) 689
HhaI GCGC 1 cut(s) 898
Hin1I GRCGYC 2 cut(s) 188, 700
Hin1II CATG 6 cut(s) 451, 608, 686, 734, 818, 1016
Hin6I GCGC 1 cut(s) 896
HinP1I GCGC 1 cut(s) 896
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 8 cut(s) 91, 123, 136, 242, 581, 986, 1022, 1070
HpaII CCGG 7 cut(s) 9, 72, 119, 131, 140, 171, 463
HphI GGTGA 4 cut(s) 325, 372, 1177, 1225
Hpy166II GTNNAC 8 cut(s) 31, 183, 300, 342, 711, 718, 853, 884
Hpy188I TCNGA 6 cut(s) 99, 221, 241, 639, 673, 1227
Hpy8I GTNNAC 8 cut(s) 31, 183, 300, 342, 711, 718, 853, 884
Hpy99I CGWCG 5 cut(s) 26, 91, 179, 190, 548
HpyCH4III ACNGT 4 cut(s) 26, 304, 715, 1165
HpyCH4IV ACGT 3 cut(s) 188, 965, 1174
HpyCH4V TGCA 5 cut(s) 444, 853, 917, 1196, 1235
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 171, 450, 599
HpyF3I CTNAG 6 cut(s) 285, 294, 498, 528, 594, 878
HpySE526I ACGT 3 cut(s) 188, 965, 1174
Hsp92I GRCGYC 2 cut(s) 188, 700
Hsp92II CATG 6 cut(s) 451, 608, 686, 734, 818, 1016
HspAI GCGC 1 cut(s) 896
Ksp22I TGATCA 1 cut(s) 685
LguI GCTCTTC 1 cut(s) 1211
LmnI GCTCC 2 cut(s) 78, 1000
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 1 cut(s) 361
MaeI CTAG 1 cut(s) 110
MaeII ACGT 3 cut(s) 188, 965, 1174
MaeIII GTNAC 4 cut(s) 390, 650, 1165, 1175
MbiI CCGCTC 1 cut(s) 81
MboII GAAGA 7 cut(s) 185, 204, 428, 626, 927, 1198, 1228
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 2 cut(s) 855, 1223
MluCI AATT 4 cut(s) 152, 1115, 1141, 1189
MlyI GAGTC 4 cut(s) 117, 130, 590, 1064
MmeI TCCRAC 1 cut(s) 347
MseI TTAA 1 cut(s) 1028
MslI CAYNNNNRTG 2 cut(s) 47, 347
MspI CCGG 7 cut(s) 9, 72, 119, 131, 140, 171, 463
MspR9I CCNGG 9 cut(s) 9, 120, 132, 247, 384, 463, 464, 999, 1077
Mva1269I GAATGC 1 cut(s) 735
MvaI CCWGG 4 cut(s) 247, 384, 999, 1077
MvnI CGCG 1 cut(s) 898
MwoI GCNNNNNNNGC 4 cut(s) 59, 171, 450, 599
NciI CCSGG 5 cut(s) 9, 120, 132, 463, 464
NlaIII CATG 6 cut(s) 451, 608, 686, 734, 818, 1016
NlaIV GGNNCC 5 cut(s) 58, 169, 200, 223, 996
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 3 cut(s) 650, 1165, 1175
PagI TCATGA 4 cut(s) 604, 682, 814, 1012
PciSI GCTCTTC 1 cut(s) 1211
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 1 cut(s) 735
PfeI GAWTC 4 cut(s) 91, 242, 986, 1022
PkrI GCNGC 2 cut(s) 167, 446
PleI GAGTC 4 cut(s) 117, 130, 589, 1064
PpsI GAGTC 4 cut(s) 117, 130, 589, 1064
Psp124BI GAGCTC 1 cut(s) 1223
Psp6I CCWGG 4 cut(s) 245, 382, 997, 1075
PspFI CCCAGC 1 cut(s) 53
PspGI CCWGG 4 cut(s) 245, 382, 997, 1075
PspN4I GGNNCC 5 cut(s) 58, 169, 200, 223, 996
PspPI GGNCC 6 cut(s) 6, 57, 83, 198, 320, 788
PsrI GAACNNNNNNTAC 2 cut(s) 344, 376
PstNI CAGNNNCTG 2 cut(s) 791, 989
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 3 cut(s) 182, 931, 1003
RsaNI GTAC 3 cut(s) 181, 930, 1002
RseI CAYNNNNRTG 2 cut(s) 47, 347
Rsr2I CGGWCCG 1 cut(s) 83
RsrII CGGWCCG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 1223
SalI GTCGAC 1 cut(s) 716
SapI GCTCTTC 1 cut(s) 1211
SaqAI TTAA 1 cut(s) 1028
SatI GCNGC 2 cut(s) 166, 445
Sau96I GGNCC 6 cut(s) 6, 57, 83, 198, 320, 788
SchI GAGTC 4 cut(s) 117, 130, 590, 1064
ScrFI CCNGG 9 cut(s) 9, 120, 132, 247, 384, 463, 464, 999, 1077
SduI GDGCHC 2 cut(s) 855, 1223
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 1 cut(s) 405
SgrAI CRCCGGYG 1 cut(s) 170
SinI GGWCC 4 cut(s) 57, 83, 198, 320
SmaI CCCGGG 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 47, 347
Sse9I AATT 4 cut(s) 152, 1115, 1141, 1189
SsiI CCGC 6 cut(s) 60, 75, 81, 146, 165, 265
SspI AATATT 2 cut(s) 892, 923
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 1223
StyD4I CCNGG 9 cut(s) 7, 118, 130, 245, 382, 461, 462, 997, 1075
TaaI ACNGT 4 cut(s) 26, 304, 715, 1165
TaiI ACGT 3 cut(s) 191, 968, 1177
TaqI TCGA 7 cut(s) 47, 68, 89, 261, 369, 492, 717
TaqII GACCGA 2 cut(s) 186, 308
TasI AATT 4 cut(s) 152, 1115, 1141, 1189
TatI WGTACW 1 cut(s) 180
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 4 cut(s) 91, 242, 986, 1022
Tru1I TTAA 1 cut(s) 1028
Tru9I TTAA 1 cut(s) 1028
TscAI CASTG 2 cut(s) 298, 759
TseFI GTSAC 3 cut(s) 650, 1165, 1175
TseI GCWGC 1 cut(s) 444
Tsp45I GTSAC 3 cut(s) 650, 1165, 1175
TspDTI ATGAA 6 cut(s) 365, 671, 803, 831, 1104, 1154
TspGWI ACGGA 5 cut(s) 75, 264, 428, 690, 1184
TspMI CCCGGG 1 cut(s) 462
TspRI CASTG 2 cut(s) 298, 759
VneI GTGCAC 1 cut(s) 851
VpaK11BI GGWCC 4 cut(s) 57, 83, 198, 320
XapI RAATTY 3 cut(s) 152, 1141, 1189
XmaI CCCGGG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 717
XspI CTAG 1 cut(s) 110
ZraI GACGTC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.