Rw1G040210

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015697
Physical Location & Seq
Forward (+)
67576079 .. 67578093
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040210.1

Sequence Viewer

Length: 1140 bp
ATGGCAGGGTCGTCGTCCTCCGGCGGAGGGCCGTCGAATCCTCCGAAAATCCTTTTGGCAAAGCCGGGACTGGTGACCGGCGCGCCGGTGGCGGGTAAATATGGGCGCGGCGGCAGTGGGGATGAGTATACGGCGTCGCTTCGGTCCCGCCTCCCTTCTATCGGATCCCTCAACCTCCTCTCTGATTCCTGGGAGTTCCACATCGACCGCTTCCTCCCGTTTTTGACTGAGAACACCGAGTTCACGGTGGTGGGGGTGATTGGTCCGCCTGGAGTCGGCAAGTCCACCATTATGAACGAGCTTTACGGCTTCGATGCCACCTCACCTGGGATGCTACCTCCATTTGCTATAGAGACTGAAGAAGGTAAAGCAATGGCAAGGCATTGTAGTGTGGGAATTGAACCTAGGGTTTCTGCTGAACGCCTTATACTTCTTGACACTCAGCCGGTTTTTAGTCCTTCTGTTTTAGCTGAGATGATGAGGCCTGATGGATCATCCACCATTCCTGTTCTAAATGCTGAATCTTTATCGGCCGAGTTAGCTCATGAACTTATGGGAATTCAGCTTGGTGTTCTTCTGACTTCCATCTGTCACATTATTCTGGTTATATCGGAAGGAGTTCATGACCATAATATGTGGCATTTAATGTCAACGGTTGACCTATTGAAACATGGTATACCTGATCCATCTTCACACACCCTTTCTCATTCTCAGAGCTCTAATATAGGGCCTGAAAAAGATAGCAAGGAAAAAGTTCCAGAAGGTGGAGAGTACATGGCTATGCCAGTTTTTGTGCACACAAAACTACGAGATCACGATCTTAGTCCACATAGTATTGTGCAACTGAGGAAGACACTTCAACAATACACCAGCACCTCTTCTTTCACAAGAGCAAAAAATGCAAATTTGTATGTAATCCCAATGAAGAGTAAAGATGACTCCCCAGGAGATCAATATGAAAGTTATACCTCTATGCTATGGAAATTGCGGGACCAGGTCTTATCTATGAATCCCCCTTCTTTCCGAAAGACTCTGACTGAACGTGACTGGTTGAAGAACTCTGCCAAGATATGGGAACTGGTGAAGAGCTCCGATTATTGCAGAATACAGCAGAACACTTCAGAGCTCTGGTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

41.58

Weight (kDa)

6.01

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015697)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33920 FvH4_7g33920
malus_domestica MD07G1308500.v1.1
prunus_persica Prupe.2G325700_v2.0.a1
pyrus_communis pycom07g28070
rosa_chinensis RchiOBHm_Chr1g0384131
rosa_laevigata RLG00000026069
rosa_multiflora Rmu_sc0005139.1_g000016 Rmu_sc0014419.1_g000001
rosa_roxburghii Rroxscaffold_4G00276960
rosa_rugosa Rorug01G0445100
rosa_samantha Rh1AG470600 Rh1BG428000 Rh1CG440700 Rh1DG461000
rosa_wichuraiana Rw1G040210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 764, 1071
AccI GTMKAC 2 cut(s) 128, 676
AccII CGCG 2 cut(s) 83, 108
AciI CCGC 8 cut(s) 24, 92, 108, 111, 148, 208, 266, 988
AclWI GGATC 4 cut(s) 159, 172, 499, 677
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 2 cut(s) 558, 904
AcuI CTGAAG 2 cut(s) 378, 1104
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 7 cut(s) 27, 92, 161, 275, 327, 764, 1071
AgsI TTSAA 4 cut(s) 401, 667, 860, 1054
AjnI CCWGG 5 cut(s) 188, 268, 325, 943, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 7 cut(s) 301, 470, 542, 565, 717, 1089, 1126
AluI AGCT 7 cut(s) 301, 470, 542, 565, 717, 1089, 1126
Alw21I GWGCWC 4 cut(s) 719, 798, 1091, 1128
Alw26I GTCTC 1 cut(s) 347
Alw44I GTGCAC 1 cut(s) 794
AlwI GGATC 4 cut(s) 159, 172, 499, 677
AoxI GGCC 4 cut(s) 29, 482, 531, 728
ApaLI GTGCAC 1 cut(s) 794
ApoI RAATTY 2 cut(s) 558, 904
AscI GGCGCGCC 1 cut(s) 81
Asp700I GAANNNNTTC 2 cut(s) 618, 753
AspA2I CCTAGG 1 cut(s) 404
AspLEI GCGC 3 cut(s) 83, 85, 108
AspS9I GGNCC 5 cut(s) 29, 144, 263, 728, 991
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 4 cut(s) 85, 268, 315, 1093
AvaII GGWCC 3 cut(s) 144, 263, 991
AvrII CCTAGG 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 798
BamHI GGATCC 1 cut(s) 164
BanII GRGCYC 3 cut(s) 719, 1091, 1128
BbsI GAAGAC 1 cut(s) 857
Bbv12I GWGCWC 4 cut(s) 719, 798, 1091, 1128
BccI CCATC 3 cut(s) 482, 593, 694
BceAI ACGGC 3 cut(s) 16, 147, 322
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 5 cut(s) 190, 270, 327, 945, 995
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 1 cut(s) 348
BisI GCNGC 2 cut(s) 109, 112
BlnI CCTAGG 1 cut(s) 404
BlsI GCNGC 2 cut(s) 110, 113
Bme1390I CCNGG 6 cut(s) 66, 190, 270, 327, 945, 995
Bme18I GGWCC 3 cut(s) 144, 263, 991
BmgT120I GGNCC 5 cut(s) 29, 144, 263, 728, 991
BmiI GGNNCC 3 cut(s) 146, 166, 992
BmrFI CCNGG 6 cut(s) 66, 190, 270, 327, 945, 995
BmsI GCATC 2 cut(s) 304, 321
BpiI GAAGAC 1 cut(s) 857
BpmI CTGGAG 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 4 cut(s) 189, 326, 404, 943
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 92, 161, 275, 327, 764, 1071
Bse118I RCCGGY 3 cut(s) 77, 85, 445
Bse1I ACTGG 4 cut(s) 75, 785, 1052, 1083
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 5 cut(s) 190, 270, 327, 945, 995
BseDI CCNNGG 4 cut(s) 189, 326, 404, 943
BseGI GGATG 3 cut(s) 127, 336, 494
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 7 cut(s) 27, 92, 161, 275, 327, 764, 1071
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 5 cut(s) 219, 455, 462, 725, 836
BseNI ACTGG 4 cut(s) 75, 785, 1052, 1083
BsePI GCGCGC 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 798
BseX3I CGGCCG 1 cut(s) 531
Bsh1236I CGCG 2 cut(s) 83, 108
Bsh1285I CGRYCG 2 cut(s) 208, 534
BshFI GGCC 4 cut(s) 31, 484, 533, 730
BsiEI CGRYCG 2 cut(s) 208, 534
BsiHKAI GWGCWC 4 cut(s) 719, 798, 1091, 1128
BsiSI CCGG 5 cut(s) 21, 65, 78, 86, 446
BslFI GGGAC 3 cut(s) 81, 130, 1004
BslI CCNNNNNNNGG 7 cut(s) 27, 92, 161, 275, 327, 764, 1071
BsmAI GTCTC 1 cut(s) 347
BsmFI GGGAC 3 cut(s) 81, 130, 1004
BsnI GGCC 4 cut(s) 31, 484, 533, 730
Bsp1286I GDGCHC 4 cut(s) 719, 798, 1091, 1128
Bsp143I GATC 6 cut(s) 164, 491, 682, 811, 817, 949
BspACI CCGC 8 cut(s) 24, 92, 108, 111, 148, 208, 266, 988
BspANI GGCC 4 cut(s) 31, 484, 533, 730
BspCNI CTCAG 5 cut(s) 220, 454, 463, 724, 837
BspFNI CGCG 2 cut(s) 83, 108
BspHI TCATGA 2 cut(s) 544, 622
BspLI GGNNCC 3 cut(s) 146, 166, 992
BspPI GGATC 4 cut(s) 159, 172, 499, 677
BspQI GCTCTTC 1 cut(s) 1079
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 3 cut(s) 77, 85, 445
BsrI ACTGG 4 cut(s) 75, 785, 1052, 1083
BssAI RCCGGY 3 cut(s) 77, 85, 445
BssECI CCNNGG 4 cut(s) 189, 326, 404, 943
BssHII GCGCGC 1 cut(s) 81
BssMI GATC 6 cut(s) 164, 491, 682, 811, 817, 949
BssNAI GTATAC 2 cut(s) 129, 677
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 1 cut(s) 404
Bst1107I GTATAC 2 cut(s) 129, 677
Bst2UI CCWGG 5 cut(s) 190, 270, 327, 945, 995
Bst4CI ACNGT 2 cut(s) 247, 655
Bst6I CTCTTC 3 cut(s) 883, 920, 1079
BstACI GRCGYC 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 899
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 6 cut(s) 228, 441, 471, 711, 821, 845
BstEII GGTNACC 1 cut(s) 73
BstF5I GGATG 3 cut(s) 127, 336, 494
BstFNI CGCG 2 cut(s) 83, 108
BstHHI GCGC 3 cut(s) 83, 85, 108
BstKTI GATC 6 cut(s) 167, 494, 685, 814, 820, 952
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 6 cut(s) 164, 491, 682, 811, 817, 949
BstMCI CGRYCG 2 cut(s) 208, 534
BstMWI GCNNNNNNNGC 3 cut(s) 89, 539, 899
BstNI CCWGG 5 cut(s) 190, 270, 327, 945, 995
BstPI GGTNACC 1 cut(s) 73
BstSCI CCNGG 6 cut(s) 64, 188, 268, 325, 943, 993
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 2 cut(s) 83, 108
BstV2I GAAGAC 1 cut(s) 857
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BstZ17I GTATAC 2 cut(s) 129, 677
BstZI CGGCCG 1 cut(s) 531
BsuRI GGCC 4 cut(s) 31, 484, 533, 730
BtsCI GGATG 3 cut(s) 127, 336, 494
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 83
CciI TCATGA 2 cut(s) 544, 622
CfoI GCGC 3 cut(s) 83, 85, 108
Cfr10I RCCGGY 3 cut(s) 77, 85, 445
Cfr13I GGNCC 5 cut(s) 29, 144, 263, 728, 991
CseI GACGC 1 cut(s) 123
CsiI ACCWGGT 1 cut(s) 993
Csp6I GTAC 1 cut(s) 772
CviAII CATG 4 cut(s) 545, 623, 671, 775
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 6 cut(s) 228, 441, 471, 711, 821, 845
DpnI GATC 6 cut(s) 166, 493, 684, 813, 819, 951
DpnII GATC 6 cut(s) 164, 491, 682, 811, 817, 949
EaeI YGGCCR 1 cut(s) 531
EagI CGGCCG 1 cut(s) 531
Eam1104I CTCTTC 3 cut(s) 883, 920, 1079
EarI CTCTTC 3 cut(s) 883, 920, 1079
EciI GGCGGA 2 cut(s) 39, 255
Ecl136II GAGCTC 3 cut(s) 717, 1089, 1126
EclXI CGGCCG 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 404
Eco147I AGGCCT 1 cut(s) 484
Eco24I GRGCYC 3 cut(s) 719, 1091, 1128
Eco47I GGWCC 3 cut(s) 144, 263, 991
Eco52I CGGCCG 1 cut(s) 531
Eco53kI GAGCTC 3 cut(s) 717, 1089, 1126
Eco57I CTGAAG 2 cut(s) 378, 1104
Eco91I GGTNACC 1 cut(s) 73
EcoICRI GAGCTC 3 cut(s) 717, 1089, 1126
EcoO109I RGGNCCY 1 cut(s) 728
EcoO65I GGTNACC 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 5 cut(s) 188, 268, 325, 943, 993
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 3 cut(s) 719, 1091, 1128
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 4 cut(s) 548, 626, 674, 778
FaqI GGGAC 3 cut(s) 81, 130, 1004
FatI CATG 4 cut(s) 544, 622, 670, 774
FauI CCCGC 3 cut(s) 85, 155, 981
FblI GTMKAC 2 cut(s) 128, 676
Fnu4HI GCNGC 2 cut(s) 109, 112
FokI GGATG 3 cut(s) 134, 343, 481
FriOI GRGCYC 3 cut(s) 719, 1091, 1128
Fsp4HI GCNGC 2 cut(s) 109, 112
FspBI CTAG 1 cut(s) 405
GlaI GCGC 3 cut(s) 82, 84, 107
GluI GCNGC 2 cut(s) 109, 112
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 4 cut(s) 31, 484, 533, 730
HapII CCGG 5 cut(s) 21, 65, 78, 86, 446
HgaI GACGC 1 cut(s) 123
HhaI GCGC 3 cut(s) 83, 85, 108
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 4 cut(s) 548, 626, 674, 778
Hin6I GCGC 3 cut(s) 81, 83, 106
HinP1I GCGC 3 cut(s) 81, 83, 106
HincII GTYRAC 2 cut(s) 651, 658
HindII GTYRAC 2 cut(s) 651, 658
HinfI GANTC 7 cut(s) 37, 185, 273, 521, 938, 1009, 1030
HpaII CCGG 5 cut(s) 21, 65, 78, 86, 446
HphI GGTGA 4 cut(s) 85, 268, 315, 1093
Hpy166II GTNNAC 8 cut(s) 129, 243, 285, 651, 658, 677, 796, 827
Hpy188III TCNNGA 5 cut(s) 434, 545, 623, 758, 815
Hpy8I GTNNAC 8 cut(s) 129, 243, 285, 651, 658, 677, 796, 827
Hpy99I CGWCG 3 cut(s) 16, 37, 139
HpyAV CCTTC 6 cut(s) 165, 356, 468, 608, 755, 1026
HpyCH4III ACNGT 2 cut(s) 247, 655
HpyCH4IV ACGT 1 cut(s) 1042
HpyCH4V TGCA 4 cut(s) 796, 841, 902, 1101
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 539, 899
HpyF3I CTNAG 6 cut(s) 228, 441, 471, 711, 821, 845
HpySE526I ACGT 1 cut(s) 1042
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 4 cut(s) 548, 626, 674, 778
HspAI GCGC 3 cut(s) 81, 83, 106
Kzo9I GATC 6 cut(s) 164, 491, 682, 811, 817, 949
LguI GCTCTTC 1 cut(s) 1079
LmnI GCTCC 1 cut(s) 1094
LweI GCATC 2 cut(s) 304, 321
MabI ACCWGGT 1 cut(s) 993
MaeI CTAG 1 cut(s) 405
MaeII ACGT 1 cut(s) 1042
MaeIII GTNAC 3 cut(s) 73, 590, 1043
MalI GATC 6 cut(s) 166, 493, 684, 813, 819, 951
MboI GATC 6 cut(s) 164, 491, 682, 811, 817, 949
MboII GAAGA 8 cut(s) 371, 566, 681, 862, 870, 937, 1066, 1096
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 4 cut(s) 719, 798, 1091, 1128
MluCI AATT 4 cut(s) 396, 558, 904, 983
MlyI GAGTC 3 cut(s) 282, 932, 1024
MroXI GAANNNNTTC 2 cut(s) 618, 753
MseI TTAA 1 cut(s) 644
MslI CAYNNNNRTG 4 cut(s) 248, 290, 387, 779
MspI CCGG 5 cut(s) 21, 65, 78, 86, 446
MspR9I CCNGG 6 cut(s) 66, 190, 270, 327, 945, 995
MvaI CCWGG 5 cut(s) 190, 270, 327, 945, 995
MvnI CGCG 2 cut(s) 83, 108
MwoI GCNNNNNNNGC 3 cut(s) 89, 539, 899
NciI CCSGG 1 cut(s) 66
NdeII GATC 6 cut(s) 164, 491, 682, 811, 817, 949
NlaIII CATG 4 cut(s) 548, 626, 674, 778
NlaIV GGNNCC 3 cut(s) 146, 166, 992
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 3 cut(s) 73, 590, 1043
OliI CACNNNNGTG 1 cut(s) 248
PagI TCATGA 2 cut(s) 544, 622
PalAI GGCGCGCC 1 cut(s) 81
PauI GCGCGC 1 cut(s) 81
PceI AGGCCT 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 618, 753
PfeI GAWTC 4 cut(s) 37, 185, 521, 1009
PflFI GACNNNGTC 1 cut(s) 995
PflMI CCANNNNNTGG 2 cut(s) 764, 1071
PkrI GCNGC 2 cut(s) 110, 113
PleI GAGTC 3 cut(s) 281, 932, 1024
PpsI GAGTC 3 cut(s) 281, 932, 1024
Psp124BI GAGCTC 3 cut(s) 719, 1091, 1128
Psp6I CCWGG 5 cut(s) 188, 268, 325, 943, 993
PspEI GGTNACC 1 cut(s) 73
PspGI CCWGG 5 cut(s) 188, 268, 325, 943, 993
PspN4I GGNNCC 3 cut(s) 146, 166, 992
PspPI GGNCC 5 cut(s) 29, 144, 263, 728, 991
PsrI GAACNNNNNNTAC 4 cut(s) 287, 319, 411, 443
PsuI RGATCY 1 cut(s) 164
PsyI GACNNNGTC 1 cut(s) 995
PteI GCGCGC 1 cut(s) 81
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 4 cut(s) 248, 290, 387, 779
SacI GAGCTC 3 cut(s) 719, 1091, 1128
SapI GCTCTTC 1 cut(s) 1079
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 2 cut(s) 109, 112
Sau3AI GATC 6 cut(s) 164, 491, 682, 811, 817, 949
Sau96I GGNCC 5 cut(s) 29, 144, 263, 728, 991
SchI GAGTC 3 cut(s) 282, 932, 1024
ScrFI CCNGG 6 cut(s) 66, 190, 270, 327, 945, 995
SduI GDGCHC 4 cut(s) 719, 798, 1091, 1128
SexAI ACCWGGT 1 cut(s) 993
SfaNI GCATC 2 cut(s) 304, 321
SfcI CTRYAG 1 cut(s) 348
SgrAI CRCCGGYG 1 cut(s) 85
SgsI GGCGCGCC 1 cut(s) 81
SinI GGWCC 3 cut(s) 144, 263, 991
SmiMI CAYNNNNRTG 4 cut(s) 248, 290, 387, 779
Sse9I AATT 4 cut(s) 396, 558, 904, 983
SseBI AGGCCT 1 cut(s) 484
SsiI CCGC 8 cut(s) 24, 92, 108, 111, 148, 208, 266, 988
SspMI CTAG 1 cut(s) 405
SstI GAGCTC 3 cut(s) 719, 1091, 1128
StuI AGGCCT 1 cut(s) 484
StyD4I CCNGG 6 cut(s) 64, 188, 268, 325, 943, 993
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 247, 655
TaiI ACGT 1 cut(s) 1045
TaqI TCGA 3 cut(s) 35, 204, 312
TaqII GACCGA 1 cut(s) 132
TasI AATT 4 cut(s) 396, 558, 904, 983
TatI WGTACW 1 cut(s) 771
TauI GCSGC 2 cut(s) 111, 114
TfiI GAWTC 4 cut(s) 37, 185, 521, 1009
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 1 cut(s) 121
TseFI GTSAC 3 cut(s) 73, 590, 1043
Tsp45I GTSAC 3 cut(s) 73, 590, 1043
TspDTI ATGAA 6 cut(s) 308, 561, 611, 938, 972, 1022
TspRI CASTG 1 cut(s) 121
Tth111I GACNNNGTC 1 cut(s) 995
Van91I CCANNNNNTGG 2 cut(s) 764, 1071
VneI GTGCAC 1 cut(s) 794
VpaK11BI GGWCC 3 cut(s) 144, 263, 991
XapI RAATTY 2 cut(s) 558, 904
XmaJI CCTAGG 1 cut(s) 404
XmiI GTMKAC 2 cut(s) 128, 676
XmnI GAANNNNTTC 2 cut(s) 618, 753
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.