Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0008850

Physical Location & Seq

Genomic Coordinates Reverse (-)

5177774 .. 5183919

Gene Structure 6146 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G101400.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

ATGAGAGAAGAGAACCCATCAGAAAGCAGAGCAAGATCGATCAAGTTTTCTGACCAAAATCAGATACCAAAGTGCCAGAATGTGAAGGGCAATAGCAACGCTTCAAAGTTAAGGTCTGCTTCTTCATGGGGTTCTCATATTGTGAAAGGGCTTGCAGGAGACAAGAAGACCAAGGTTCAACCCATAGTGACAAACAAGAAACCGCCGCTAATGGGCTCTGATATGGCGAACCAGAAGAATTCATTTGTGCCTTCTCATCCACGCGTCAAGCGTTCGCTCATTGGAGACTTGTCATGCTCAGTCAATGGTAATCAAGTTCATCCTCAAATGCATCCCACCCATCGGAGACAGTCCTCTAGGGACTTATTTATTGAGCTTGATCATTTGAGAAACTTACTTCGAGAATCTAAGGAAAGAGAGTTCCAGTTGCAAGCTGAGTTATCGGAGTGTAAGCGAAACCCGAAAGTTTTAGACCTTGAGAGGGAGCTTGAGGTGAAAAGGATTGAGCTTGATGGTCTAGCTCGAAAAGTTGAGTTGCTGGAAGAAGAAAAAACTAGCCTCTCTGAGCAATTATCGGCATTAACATCGATTTTGGACAGGAATGAAGGAGTGACATTGAAAAAAGAAGAACAAGAAAGTTCAGTGGCATCAGCATCAGGGAGTGTGGAAATGGAGGTTGTGGAGTTGAGGCGCCTGAACAAGGAGCTACAGCTTCAGAAGAGAAATCTTGCTTGCAAGCTTTCGTCTGTGACATCCCAGTTGGCTTCTCTAGCAAAGGCTTCTGAGAGTGACATTGTTGAGAAGATCAAAGCTGAGGCATCTGCGCTAAGACATACAAATGAAGATTTGTGTAAACAAGTTGAAGGTTTACAAATGAGCAGGTTGAATGAGGTTGAGGAGCTAGCCTACCTGAGGTGGGTGAATTCGTGTTTACGAAATGAGTTGCAGAACTCCAATTCATGCTCCACAACAAATTCTGATAAGCCATTAAGCCCCGGTTCGTTTGAGAGGAGTAGTAAATCTGCTGGAGCATTGCCATCTAGAAGCAGCGAGTACTTAGAATACGGTAGTGTAAAGAGATTGAATCTAATAAAAAAGCTAAAGAAATGGCCTATTGCTGATGAGGACCTGCCCAACCTAGAATGCCCAGATGGTCTCTTGGATAAAAGTTGGGTTGATTCAGAGGAAGGTAGAAGTCCTAGAAGAAGACACTCAATAAGTGGATCTAAATGCTGTGCAGAAGAGTTGGTGCAGAGTAATAAGAGAAGGCAATCTGATGGTTTTATGTGTGCTCAAGAAATGGAGAAGGATACAGAGCCAGTAGCTTCTGAAAATTTTGATTTATTCTTTGGAAATTGTCATGAAATTAACAAAATTCCAGCTTCATTAGATGTGGAGAAAAGGGCCTTGAGAATTCCAAATCCCCCTCCAAGGCCTTCATGTTCCATTTCTCGTGGAACCAAAGTGGATGGTTCTGCCCAAGTTCCACCGCCACCGCCTCCACCACCCCCGCCACCTCCTCCCAAGTTTGCAATGAAGACTAGTACCACAGGAATGGTACAAAGAGCCCCACAAGTAGTTGAGTTTTATCATTCACTCATGAAGAGAGATTCTAGGAAGGATTCTTCAAATGGAGGAGTTTGTGATGGCCCAGATGTTGCAAATGTTCGTAGTAGCATGATTGGAGAAATTGAGAACCGATCATCACATTTGCTTGCTATAAAGGCAGATGTTGAGACTCAAGGAGAATTTGTGAATTCGTTGATCAGAGAGGTGAACAACGCAGTTTATCAAAACATTGACGATGTTGTGGCCTTTGTGAAGTGGCTCGATGACGAACTTTGCTTTCTTGTGGATGAGAGGGCAGTCCTAAAGCATTTTGATTGGCCAGAAAAGAAAGCCGACACACTACGAGAAGCAGCATTCGGTTATAGAGATCTCAAGAAATTGGAGTCTGAAGTGTCTTCATACAAGGAAGATATAAGACTGCCTTGTGATATTGCCCTAAAGAAAATGGTCGCATTGTCTGAGAAGATGGAGCGTACTGTTTATAACCTTCTCCGGACAAGGGAGCCCTTGATGCGTCATTGCAAGGAGTTTCAGATACCCACAGATTGGATGCTTGACAATGGAATTTTAAGCAAGATAAAGTTTGGCTCAGTCAAGTTGGCAAAGATGTACATGAAGAGGGTGGCCATGGAACTTCAGTCAAAGGCGGCAGCGGAGAAAGATCCTGCTATGGATTACATGCTGCTTCAGGGAGTGAGATTTGCTTTCAGAATTCATCAGGGGGATTCGACGCAGACACGATGCATGCGTTCGAGGAACTTCGTTACCTTGCTCATCTTCTTAACAAAAAGTAAAGAGGGAAAGGCGGTGGAAGGGGAAGGATTTAACAGAACATTTTTGGCTTACCATCTCAGTAAGCTTCATACGGAAACGTATAATACATTCCGTAGAAAGCAATCTGGCCAGACAACGTGTTTTGGTGTAGTTTTGAGTTTGGCTGTTAATGAGCTTGGTGTGAAAAATAGGAAAGGATTTAGGTTTGGGTTAATACTTAAAATTACCAGAAGTGAAATGATATGTTGCATGTATTGCAACGCTTCTTGTAATATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

874

Amino Acids

98.54

Weight (kDa)

8.92

Isoelectric Point (pI)

59.1

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008850)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11100 FvH4_3g11100 FvH4_3g11100 FvH4_3g11100
malus_domestica	MD05G1264200.v1.1 MD10G1242300.v1.1
prunus_persica	Prupe.4G101400_v2.0.a1 Prupe.4G101400_v2.0.a1 Prupe.4G101400_v2.0.a1
pyrus_communis	pycom05g24830 pycom05g24840 pycom10g20290
rosa_chinensis	RchiOBHm_Chr5g0017991
rosa_laevigata	RLG00000032329
rosa_multiflora	Rmu_sc0010280.1_g000003 Rmu_sc0011118.1_g000004
rosa_roxburghii	Rroxscaffold_1G00059610
rosa_rugosa	Rorug05G0039500
rosa_samantha	Rh5AG132400 Rh5BG130500 Rh5CG142900 Rh5DG132500
rosa_wichuraiana	Rw5G011530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2052
Acc36I	ACCTGC	2 cut(s)	870, 1137
AccB1I	GGYRCC	1 cut(s)	690
AccB7I	CCANNNNNTGG	1 cut(s)	2115
AccII	CGCG	1 cut(s)	264
AccIII	TCCGGA	1 cut(s)	2061
AciI	CCGC	8 cut(s)	203, 206, 1490, 1496, 1511, 2216, 2222, 2375
AclWI	GGATC	2 cut(s)	1231, 2225
AcoI	YGGCCR	3 cut(s)	1886, 2193, 2470
AcuI	CTGAAG	4 cut(s)	698, 1977, 2189, 2240
AcyI	GRCGYC	1 cut(s)	691
AfaI	GTAC	5 cut(s)	1055, 1546, 1560, 2044, 2180
AfiI	CCNNNNNNNGG	9 cut(s)	212, 342, 481, 700, 912, 916, 1431, 2068, 2115
AflIII	ACRYGT	2 cut(s)	262, 2480
AgsI	TTSAA	7 cut(s)	105, 179, 619, 863, 886, 1084, 1629
AhlI	ACTAGT	1 cut(s)	1541
AloI	GAACNNNNNNTCC	2 cut(s)	404, 436
Alw21I	GWGCWC	1 cut(s)	1294
Alw26I	GTCTC	5 cut(s)	153, 279, 340, 1160, 1730
AlwI	GGATC	2 cut(s)	1231, 2225
Aor13HI	TCCGGA	1 cut(s)	2061
AoxI	GGCC	8 cut(s)	1109, 1404, 1433, 1648, 1812, 1886, 2193, 2470
ApeKI	GCWGC	4 cut(s)	1047, 1919, 2219, 2251
ArsI	GACNNNNNNTTYG	2 cut(s)	98, 130
AspLEI	GCGC	2 cut(s)	693, 826
AspS9I	GGNCC	3 cut(s)	1126, 1404, 1649
AsuC2I	CCSGG	1 cut(s)	996
AsuHPI	GGTGA	3 cut(s)	505, 931, 1786
AsuNHI	GCTAGC	1 cut(s)	901
AvaII	GGWCC	1 cut(s)	1126
AxyI	CCTNAGG	1 cut(s)	911
BaeI	ACNNNNGTAYC	2 cut(s)	56, 89
BalI	TGGCCA	3 cut(s)	1888, 2195, 2472
BanI	GGYRCC	1 cut(s)	690
BanII	GRGCYC	3 cut(s)	218, 1570, 2076
BauI	CACGAG	1 cut(s)	1452
BbsI	GAAGAC	4 cut(s)	173, 1213, 1544, 1956
Bbv12I	GWGCWC	1 cut(s)	1294
BbvCI	CCTCAGC	1 cut(s)	813
BbvI	GCAGC	4 cut(s)	1059, 1931, 2231, 2238
BcgI	CGANNNNNNTGC	2 cut(s)	567, 601
BciVI	GTATCC	1 cut(s)	1303
BclI	TGATCA	2 cut(s)	379, 1764
BcnI	CCSGG	1 cut(s)	996
BcoDI	GTCTC	5 cut(s)	153, 279, 340, 1160, 1730
BcuI	ACTAGT	1 cut(s)	1541
BfmI	CTRYAG	1 cut(s)	707
BfoI	RGCGCY	1 cut(s)	694
BfuAI	ACCTGC	2 cut(s)	870, 1137
BfuI	GTATCC	1 cut(s)	1303
BglII	AGATCT	1 cut(s)	1936
BisI	GCNGC	6 cut(s)	206, 1048, 1920, 2217, 2220, 2252
BlsI	GCNGC	6 cut(s)	207, 1049, 1921, 2218, 2221, 2253
BmcAI	AGTACT	1 cut(s)	1055
Bme1390I	CCNGG	1 cut(s)	996
Bme18I	GGWCC	1 cut(s)	1126
BmgT120I	GGNCC	3 cut(s)	1126, 1404, 1649
BmiI	GGNNCC	3 cut(s)	692, 1459, 2073
BmrFI	CCNGG	1 cut(s)	996
BmrI	ACTGGG	1 cut(s)	751
BmsI	GCATC	7 cut(s)	340, 656, 662, 827, 2070, 2109, 2300
BmtI	GCTAGC	1 cut(s)	905
BmuI	ACTGGG	1 cut(s)	751
BpiI	GAAGAC	4 cut(s)	173, 1213, 1544, 1956
BpmI	CTGGAG	1 cut(s)	1047
Bpu10I	CCTNAGC	1 cut(s)	813
BpuEI	CTTGAG	6 cut(s)	497, 509, 1278, 1429, 1725, 1925
BpuMI	CCSGG	1 cut(s)	996
Bsa29I	ATCGAT	2 cut(s)	38, 587
BsaHI	GRCGYC	1 cut(s)	691
BsaI	GGTCTC	1 cut(s)	1160
BsaJI	CCNNGG	4 cut(s)	171, 994, 1430, 2196
BsaWI	WCCGGW	1 cut(s)	2061
BsaXI	ACNNNNNCTCC	2 cut(s)	1737, 1767
Bsc4I	CCNNNNNNNGG	9 cut(s)	212, 342, 481, 700, 912, 916, 1431, 2068, 2115
Bse1I	ACTGG	3 cut(s)	424, 757, 1319
Bse21I	CCTNAGG	1 cut(s)	911
Bse3DI	GCAATG	3 cut(s)	1031, 1539, 2086
BseAI	TCCGGA	1 cut(s)	2061
BseCI	ATCGAT	2 cut(s)	38, 587
BseDI	CCNNGG	4 cut(s)	171, 994, 1430, 2196
BseGI	GGATG	7 cut(s)	256, 319, 331, 752, 1474, 1861, 2124
BseLI	CCNNNNNNNGG	9 cut(s)	212, 342, 481, 700, 912, 916, 1431, 2068, 2115
BseMI	GCAATG	3 cut(s)	1031, 1539, 2086
BseMII	CTCAG	9 cut(s)	312, 426, 555, 774, 804, 902, 2019, 2172, 2434
BseNI	ACTGG	3 cut(s)	424, 757, 1319
BseRI	GAGGAG	4 cut(s)	911, 1024, 1509, 1650
BseXI	GCAGC	4 cut(s)	1059, 1931, 2231, 2238
BsgI	GTGCAG	2 cut(s)	1257, 1271
Bsh1236I	CGCG	1 cut(s)	264
BshFI	GGCC	8 cut(s)	1111, 1406, 1435, 1650, 1814, 1888, 2195, 2472
BshNI	GGYRCC	1 cut(s)	690
BshVI	ATCGAT	2 cut(s)	38, 587
BsiHKAI	GWGCWC	1 cut(s)	1294
BsiSI	CCGG	2 cut(s)	996, 2062
BslFI	GGGAC	1 cut(s)	374
BslI	CCNNNNNNNGG	9 cut(s)	212, 342, 481, 700, 912, 916, 1431, 2068, 2115
BsmAI	GTCTC	5 cut(s)	153, 279, 340, 1160, 1730
BsmFI	GGGAC	1 cut(s)	374
BsmI	GAATGC	2 cut(s)	1148, 1922
BsnI	GGCC	8 cut(s)	1111, 1406, 1435, 1650, 1814, 1888, 2195, 2472
Bso31I	GGTCTC	1 cut(s)	1160
Bsp1286I	GDGCHC	4 cut(s)	218, 1294, 1570, 2076
Bsp13I	TCCGGA	1 cut(s)	2061
Bsp1407I	TGTACA	1 cut(s)	2178
Bsp143I	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
Bsp19I	CCATGG	1 cut(s)	2196
BspACI	CCGC	8 cut(s)	203, 206, 1490, 1496, 1511, 2216, 2222, 2375
BspANI	GGCC	8 cut(s)	1111, 1406, 1435, 1650, 1814, 1888, 2195, 2472
BspCNI	CTCAG	9 cut(s)	311, 427, 556, 775, 805, 903, 2020, 2171, 2433
BspDI	ATCGAT	2 cut(s)	38, 587
BspEI	TCCGGA	1 cut(s)	2061
BspFNI	CGCG	1 cut(s)	264
BspHI	TCATGA	2 cut(s)	1360, 1599
BspLI	GGNNCC	3 cut(s)	692, 1459, 2073
BspMI	ACCTGC	2 cut(s)	870, 1137
BspOI	GCTAGC	1 cut(s)	905
BspPI	GGATC	2 cut(s)	1231, 2225
BspT107I	GGYRCC	1 cut(s)	690
BspTNI	GGTCTC	1 cut(s)	1160
BsrDI	GCAATG	3 cut(s)	1031, 1539, 2086
BsrGI	TGTACA	1 cut(s)	2178
BsrI	ACTGG	3 cut(s)	424, 757, 1319
BssECI	CCNNGG	4 cut(s)	171, 994, 1430, 2196
BssMI	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
BssNI	GRCGYC	1 cut(s)	691
BssSI	CACGAG	1 cut(s)	1452
BssT1I	CCWWGG	3 cut(s)	171, 1430, 2196
Bst2BI	CACGAG	1 cut(s)	1452
Bst4CI	ACNGT	3 cut(s)	351, 1067, 2047
Bst6I	CTCTTC	5 cut(s)	3, 713, 1236, 1598, 2180
BstACI	GRCGYC	1 cut(s)	691
BstAPI	GCANNNNNTGC	1 cut(s)	2598
BstAUI	TGTACA	1 cut(s)	2178
BstC8I	GCNNGC	7 cut(s)	153, 432, 733, 737, 903, 1716, 2315
BstDSI	CCRYGG	1 cut(s)	2196
BstENI	CCTNNNNNAGG	2 cut(s)	698, 910
BstF5I	GGATG	7 cut(s)	256, 319, 331, 752, 1474, 1861, 2124
BstFNI	CGCG	1 cut(s)	264
BstH2I	RGCGCY	1 cut(s)	694
BstHHI	GCGC	2 cut(s)	693, 826
BstKTI	GATC	9 cut(s)	38, 42, 382, 807, 1226, 1703, 1767, 1939, 2233
BstMAI	GTCTC	5 cut(s)	153, 279, 340, 1160, 1730
BstMBI	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
BstMWI	GCNNNNNNNGC	4 cut(s)	770, 1724, 2080, 2598
BstNSI	RCATGY	3 cut(s)	2251, 2317, 2596
BstSCI	CCNGG	1 cut(s)	994
BstSFI	CTRYAG	1 cut(s)	707
BstUI	CGCG	1 cut(s)	264
BstV1I	GCAGC	4 cut(s)	1059, 1931, 2231, 2238
BstV2I	GAAGAC	4 cut(s)	173, 1213, 1544, 1956
BstX2I	RGATCY	3 cut(s)	1223, 1936, 2230
BstXI	CCANNNNNNTGG	1 cut(s)	1555
BstYI	RGATCY	3 cut(s)	1223, 1936, 2230
Bsu15I	ATCGAT	2 cut(s)	38, 587
Bsu36I	CCTNAGG	1 cut(s)	911
BsuI	GTATCC	1 cut(s)	1303
BsuRI	GGCC	8 cut(s)	1111, 1406, 1435, 1650, 1814, 1888, 2195, 2472
BsuTUI	ATCGAT	2 cut(s)	38, 587
BtgI	CCRYGG	1 cut(s)	2196
BtsCI	GGATG	7 cut(s)	256, 319, 331, 752, 1474, 1861, 2124
BtsIMutI	CAGTG	1 cut(s)	648
BveI	ACCTGC	2 cut(s)	870, 1137
Cac8I	GCNNGC	7 cut(s)	153, 432, 733, 737, 903, 1716, 2315
CciI	TCATGA	2 cut(s)	1360, 1599
CfoI	GCGC	2 cut(s)	693, 826
Cfr13I	GGNCC	3 cut(s)	1126, 1404, 1649
ClaI	ATCGAT	2 cut(s)	38, 587
CseI	GACGC	3 cut(s)	253, 2072, 2308
Csp6I	GTAC	5 cut(s)	1054, 1545, 1559, 2043, 2179
CviQI	GTAC	5 cut(s)	1054, 1545, 1559, 2043, 2179
DinI	GGCGCC	1 cut(s)	692
DpnI	GATC	9 cut(s)	37, 41, 381, 806, 1225, 1702, 1766, 1938, 2232
DpnII	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
EaeI	YGGCCR	3 cut(s)	1886, 2193, 2470
Eam1104I	CTCTTC	5 cut(s)	3, 713, 1236, 1598, 2180
EarI	CTCTTC	5 cut(s)	3, 713, 1236, 1598, 2180
Eco130I	CCWWGG	3 cut(s)	171, 1430, 2196
Eco147I	AGGCCT	1 cut(s)	1435
Eco24I	GRGCYC	3 cut(s)	218, 1570, 2076
Eco31I	GGTCTC	1 cut(s)	1160
Eco47I	GGWCC	1 cut(s)	1126
Eco57I	CTGAAG	4 cut(s)	698, 1977, 2189, 2240
Eco81I	CCTNAGG	1 cut(s)	911
EcoNI	CCTNNNNNAGG	2 cut(s)	698, 910
EcoO109I	RGGNCCY	2 cut(s)	1126, 1404
EcoRI	GAATTC	5 cut(s)	238, 922, 1413, 1756, 2280
EcoT14I	CCWWGG	3 cut(s)	171, 1430, 2196
EcoT22I	ATGCAT	2 cut(s)	333, 2315
EcoT38I	GRGCYC	3 cut(s)	218, 1570, 2076
EgeI	GGCGCC	1 cut(s)	692
EheI	GGCGCC	1 cut(s)	692
ErhI	CCWWGG	3 cut(s)	171, 1430, 2196
FalI	AAGNNNNNCTT	4 cut(s)	103, 135, 1975, 2007
FaqI	GGGAC	1 cut(s)	374
FauI	CCCGC	1 cut(s)	1518
FbaI	TGATCA	2 cut(s)	379, 1764
Fnu4HI	GCNGC	6 cut(s)	206, 1048, 1920, 2217, 2220, 2252
FokI	GGATG	7 cut(s)	243, 306, 318, 739, 1481, 1868, 2131
FriOI	GRGCYC	3 cut(s)	218, 1570, 2076
Fsp4HI	GCNGC	6 cut(s)	206, 1048, 1920, 2217, 2220, 2252
GlaI	GCGC	2 cut(s)	692, 825
GluI	GCNGC	6 cut(s)	206, 1048, 1920, 2217, 2220, 2252
GsuI	CTGGAG	1 cut(s)	1047
HaeII	RGCGCY	1 cut(s)	694
HaeIII	GGCC	8 cut(s)	1111, 1406, 1435, 1650, 1814, 1888, 2195, 2472
HapII	CCGG	2 cut(s)	996, 2062
HgaI	GACGC	3 cut(s)	253, 2072, 2308
HhaI	GCGC	2 cut(s)	693, 826
Hin1I	GRCGYC	1 cut(s)	691
Hin6I	GCGC	2 cut(s)	691, 824
HinP1I	GCGC	2 cut(s)	691, 824
HindIII	AAGCTT	2 cut(s)	737, 2426
HinfI	GANTC	8 cut(s)	404, 1084, 1178, 1610, 1622, 1738, 1952, 2294
HpaII	CCGG	2 cut(s)	996, 2062
HphI	GGTGA	3 cut(s)	505, 931, 1786
Hpy166II	GTNNAC	4 cut(s)	854, 869, 932, 1777
Hpy188III	TCNNGA	7 cut(s)	401, 1041, 1295, 1361, 1600, 1942, 2062
Hpy8I	GTNNAC	4 cut(s)	854, 869, 932, 1777
Hpy99I	CGWCG	1 cut(s)	2302
HpyCH4III	ACNGT	3 cut(s)	351, 1067, 2047
HpyCH4IV	ACGT	2 cut(s)	2441, 2480
HpyF10VI	GCNNNNNNNGC	4 cut(s)	770, 1724, 2080, 2598
HpySE526I	ACGT	2 cut(s)	2441, 2480
Hsp92I	GRCGYC	1 cut(s)	691
HspAI	GCGC	2 cut(s)	691, 824
KasI	GGCGCC	1 cut(s)	690
Kpn2I	TCCGGA	1 cut(s)	2061
Ksp22I	TGATCA	2 cut(s)	379, 1764
Kzo9I	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
LmnI	GCTCC	7 cut(s)	484, 703, 898, 968, 1028, 2038, 2071
Lsp1109I	GCAGC	4 cut(s)	1059, 1931, 2231, 2238
LweI	GCATC	7 cut(s)	340, 656, 662, 827, 2070, 2109, 2300
MaeII	ACGT	2 cut(s)	2441, 2480
MaeIII	GTNAC	5 cut(s)	187, 610, 748, 788, 2332
MalI	GATC	9 cut(s)	37, 41, 381, 806, 1225, 1702, 1766, 1938, 2232
MboI	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
MflI	RGATCY	3 cut(s)	1223, 1936, 2230
MhlI	GDGCHC	4 cut(s)	218, 1294, 1570, 2076
MlsI	TGGCCA	3 cut(s)	1888, 2195, 2472
MluI	ACGCGT	1 cut(s)	262
MluNI	TGGCCA	3 cut(s)	1888, 2195, 2472
Mly113I	GGCGCC	1 cut(s)	691
MlyI	GAGTC	2 cut(s)	1732, 1961
Mox20I	TGGCCA	3 cut(s)	1888, 2195, 2472
Mph1103I	ATGCAT	2 cut(s)	333, 2315
MroI	TCCGGA	1 cut(s)	2061
MscI	TGGCCA	3 cut(s)	1888, 2195, 2472
MslI	CAYNNNNRTG	2 cut(s)	837, 1553
Msp20I	TGGCCA	3 cut(s)	1888, 2195, 2472
MspA1I	CMGCKG	1 cut(s)	2222
MspI	CCGG	2 cut(s)	996, 2062
MspR9I	CCNGG	1 cut(s)	996
Mva1269I	GAATGC	2 cut(s)	1148, 1922
MvnI	CGCG	1 cut(s)	264
MwoI	GCNNNNNNNGC	4 cut(s)	770, 1724, 2080, 2598
NarI	GGCGCC	1 cut(s)	691
NciI	CCSGG	1 cut(s)	996
NcoI	CCATGG	1 cut(s)	2196
NdeII	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
NheI	GCTAGC	1 cut(s)	901
NlaIV	GGNNCC	3 cut(s)	692, 1459, 2073
NmuCI	GTSAC	4 cut(s)	187, 610, 748, 788
NsiI	ATGCAT	2 cut(s)	333, 2315
NspI	RCATGY	3 cut(s)	2251, 2317, 2596
PaeI	GCATGC	1 cut(s)	2317
PagI	TCATGA	2 cut(s)	1360, 1599
PceI	AGGCCT	1 cut(s)	1435
PcsI	WCGNNNNNNNCGW	2 cut(s)	268, 2314
PctI	GAATGC	2 cut(s)	1148, 1922
PfeI	GAWTC	6 cut(s)	404, 1084, 1178, 1610, 1622, 2294
PflMI	CCANNNNNTGG	1 cut(s)	2115
PkrI	GCNGC	6 cut(s)	207, 1049, 1921, 2218, 2221, 2253
PleI	GAGTC	2 cut(s)	1732, 1960
PluTI	GGCGCC	1 cut(s)	694
PpsI	GAGTC	2 cut(s)	1732, 1960
PpuMI	RGGWCCY	1 cut(s)	1126
PsiI	TTATAA	1 cut(s)	2052
Psp5II	RGGWCCY	1 cut(s)	1126
PspN4I	GGNNCC	3 cut(s)	692, 1459, 2073
PspPI	GGNCC	3 cut(s)	1126, 1404, 1649
PspPPI	RGGWCCY	1 cut(s)	1126
PsuI	RGATCY	3 cut(s)	1223, 1936, 2230
RsaI	GTAC	5 cut(s)	1055, 1546, 1560, 2044, 2180
RsaNI	GTAC	5 cut(s)	1054, 1545, 1559, 2043, 2179
RseI	CAYNNNNRTG	2 cut(s)	837, 1553
SatI	GCNGC	6 cut(s)	206, 1048, 1920, 2217, 2220, 2252
Sau3AI	GATC	9 cut(s)	35, 39, 379, 804, 1223, 1700, 1764, 1936, 2230
Sau96I	GGNCC	3 cut(s)	1126, 1404, 1649
ScaI	AGTACT	1 cut(s)	1055
SchI	GAGTC	2 cut(s)	1732, 1961
ScrFI	CCNGG	1 cut(s)	996
SduI	GDGCHC	4 cut(s)	218, 1294, 1570, 2076
SfaNI	GCATC	7 cut(s)	340, 656, 662, 827, 2070, 2109, 2300
SfcI	CTRYAG	1 cut(s)	707
SfoI	GGCGCC	1 cut(s)	692
SinI	GGWCC	1 cut(s)	1126
SmiMI	CAYNNNNRTG	2 cut(s)	837, 1553
SmlI	CTYRAG	6 cut(s)	476, 488, 1293, 1408, 1740, 1940
SmoI	CTYRAG	6 cut(s)	476, 488, 1293, 1408, 1740, 1940
SpeI	ACTAGT	1 cut(s)	1541
SphI	GCATGC	1 cut(s)	2317
SseBI	AGGCCT	1 cut(s)	1435
SsiI	CCGC	8 cut(s)	203, 206, 1490, 1496, 1511, 2216, 2222, 2375
SspDI	GGCGCC	1 cut(s)	690
StuI	AGGCCT	1 cut(s)	1435
StyD4I	CCNGG	1 cut(s)	994
StyI	CCWWGG	3 cut(s)	171, 1430, 2196
TaaI	ACNGT	3 cut(s)	351, 1067, 2047
TaiI	ACGT	2 cut(s)	2444, 2483
TaqI	TCGA	7 cut(s)	38, 400, 523, 587, 1830, 2297, 2321
TatI	WGTACW	2 cut(s)	1053, 2178
TauI	GCSGC	2 cut(s)	208, 2219
TfiI	GAWTC	6 cut(s)	404, 1084, 1178, 1610, 1622, 2294
TscAI	CASTG	1 cut(s)	648
TseFI	GTSAC	4 cut(s)	187, 610, 748, 788
TseI	GCWGC	4 cut(s)	1047, 1919, 2219, 2251
Tsp45I	GTSAC	4 cut(s)	187, 610, 748, 788
TspGWI	ACGGA	2 cut(s)	2444, 2450
TspRI	CASTG	1 cut(s)	648
Van91I	CCANNNNNTGG	1 cut(s)	2115
VpaK11BI	GGWCC	1 cut(s)	1126
XagI	CCTNNNNNAGG	2 cut(s)	698, 910
XbaI	TCTAGA	1 cut(s)	1040
XceI	RCATGY	3 cut(s)	2251, 2317, 2596
ZrmI	AGTACT	1 cut(s)	1055
Zsp2I	ATGCAT	2 cut(s)	333, 2315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.