Prupe.5G049600_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001588
Physical Location & Seq
Forward (+)
5239502 .. 5258965
19464 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G049600.16

Sequence Viewer

Length: 4344 bp
ATGCCTCCTTCACAAATGAAATGGTACCTTTGGTGTTGCATTCTGGCGGGGCATGTTTGGATATCTGCCCTTTCTCTTACTTCAGGGAAGTACAGAAGCACAGCCAATGGTTCAGAAGAGTGGTTGCACAATGTCAGCTCAGATATAATAGAAGATTTTCAGAGAAGCACCTCAACTTATAAGGTCACAAAGTTATCATCCCTGTTAAATGATTCTGTATCTTGTGAGGACCTGGAAGGTGTTGGATCATTCAATACTACATGCTTGTTAAACTCAAACTTGAATTTCAGTTCGGATCTTTACATATATGGTACTGGAAACCTGGAGATTCTCCCTCATGTATCAATTGTCTGTCCAATTGAAGGCTGCATGATTACATTTAATATGTCAGGAAATGTTAAAATTGGTCAATTTGCTGCAATAGTTGCTGGTTCTGTAGTTTTTTCTGCTGCCAATCTGACTATGGAATATAATTCTTCTATAAATACAACATCTTTGGGTGGATTACCGCCTTCTCAAACTAGTGGAACTCCAGTGGGCTATGATGGAGGTGGTGGAGGGCATGGTGGCCGAGGTGCTTCCTGCTTGAAAAATAATCAGTCAAGTTTTTGGGGTGGTGATGTTTATACGTGGTCAACATTGTCCGAGCCGTGGAGTTATGGGAGTAAGGGTAGGGGCTTATCTACTAAAATTCCATTTGGAGGGAATGGTGGAGGACGTGTCAAGCTTCAAGTGAAGGATATGCTGTATATGAATGGGTCTGTAACTGCAGAAGGGGGGGATGGTGGGACAACTGGCGGAGGAGGATCTGGTGGAAGTATTTCTGTACATGCTGTAAAGTTGAAGGGATATGGTACCATAAGTGCTGCTGGTGGAAGAGGATGGGGTGGAGGTGGCGGTGGAAGGTTATCACTTGATTGTTACAGCATACAAGAAGATGTAAAAGTCACTGTACATGGTGGTTTGAGTATCGGTTGTCCTGGGAATGCTGGAGCTGCTGGCACATACTTTAATGCCGATTTACTTAGTTTAAGGGTTGGAAATGACAATATCACAACAGAAACTGAAACCCCTTTGCTCGACTTTCCTACCAGCCCGTTATGGTCACATGTTTTTGTGGAGAATAATGCAAAAGTGTTGGTTCCACTTCTTTGGACCAGAGTTCAGGTGAGAGGCCAAATTAGTCTATACCGTGGGGGCAGCATCATCTTTGGATTGTCCGAGTATCCAATTTCAGAATTTGAGCTTGTTGCTGAAGAACTTCTGATGAGTGATTCCATCATAAAGGTTTTTGGTGCATTTAGAGTCGCTGTCAAGATGTTACTCATGTGGAACTCCAAAATTCAAATAGATGGTGGTGGAAATCCCATTGTTACTGCTTCAGTCCTTGAAGTCAGGAATCTGATTGTTCTGAGGCAAAACTCTGTCATTAGTTCAAATACAAACTTGGGTGTATATGGCCAGGGACTACTGAAGTTGACTGGTCATGGTGATGCAATAAAAGCCCAGAGGCTCTCCTTGTCTCTCTTTTATAACATCACTGTTGGTGCTGGTTCTTTACTTCAGGCTCCATTGGATGATGATGCTAACAGAAATGTGGTTACAAAATTGCTTTGTGAGAGTCAAGCATGTCCATTGGATTTGATTACTCCACCGGATGATTGCCATGTTAATTATACACTCTCCTTTTCACTTCAAATTTGTCGTGTTGAGGACCTTCTTGTTGATGGTGTCGTGAAGGGAAGTGTAATTCACATCCACAGGGCGAGGACTATTATTGTAGATAATAATGGCCTGATCACTGCATCAGAACTAGGTTGCAGTAAAGGTATTGGAAGTGGAAACTTTTCAAATGGAGCTGGTAGTGGTGCTGGACACGGGGGAAGAGGGGGCTCTGGATACTTTAATGGGAGGGTGTGCAATGGTGGTAATGAATATGGCAATGCTGATCTCCCATGTGAATTGGGGAGTGGAGCTGAGGGTCCCAGTCCGTCATATGGGAATGTGGTTGGAGGGGGAATGATTGTGATGGGATCCATCCAGTGGCCACTCTTAAAGCTTGATGTCTTTGGAACATTGAGTGCTGATGGTCAAAGCTTTCATAAAGCAGCTAGAAATGGTAATGGTACTTTGATTGGCGGACTTGGTGGAGGTTCAGGTGGGACAATCCTTATTTTCCTTCAAGAGCTTGGATTACTTGCACAGAATTCATCTTTATCTGTTGCTGGGGGCAAAGGTGGTCCTCTTGGTGGAGGTGGGGGTGGGGGTGGAAGAGTTCATTTTCATTGGTCTAAGATAGATTTTGAGGATGAATATGTTCCGGTTGCTAGTATAAGTGGCTCCATTAACAGCAGTGGAGGTGCTGGGGATGATGGAGGTCATCATGGAAGCGATGGAACTATCACTGGGAAAAAATGCCCAAAGGGCCTTTATGGAACCTTCTGCAAGGAATGCCCTGTTGGCACATTCAAAAATGTTGATGGTTCTGATGCACATCTTTGTATTCCTTGTTCTGTTGATCTTCTTCCAAGTCGTGCTGAGTTCATCTATGTGCGAGGCGGGGTGACTCAGCCATCATGTCCCTATAAATGTGTATCTGACAATTATAGGATGCCAAAATGCTACACACCTCTCGAGGAGCTTTTGTATACTTTCGGGGGGCCCTGGCCTTTTGCAATTTTATTGTCATGCGTTTTGGTGGTTCTGGCTTTGTTACTGAGGACTTTGAGAATCAAATTGGTTGGTTCATGTTCATATCACAGGGCTGGTTCAATTGAACAGCACAGTCATCATCAATTCCCATGTCTTCTTTCCCTATCTGAGGTACGGGGAACCAGAGTTGAAGAAACTCAAAGCCATGTTCATCGAATGTACTTCATGGGTCCTAATACTTTCAGAGAACCATGGCATCTTCCTTACTCTCCTCCTAATGCAATCATTGAAATTGTATATGAAGATGCATTTAACAGATTTATTGATGAGATCAATTCTGTTGCTGCATATGATTGGTGGGAAGGGTCTGTGCATAGTATACTTTCAGTTCTCGCGTACCCTTGTTCCTGGTCCTGGAAACAGTGGCGGCAAAGAAATAAAGTTCATCGCCTTCAGGAATATGTGAAGTCTGAATATGACCATTCATGTCTTCGCTCTTGCAGATCTCGAGCCTTGTACAAAGGGATGAAGGTTGGAGCAACACCAGACTTAATGTTGGCATATATTGATTTTTTCCTTGGTGGTGATGAGAAGCGTCTAGATATGGTATCAATCATACAGAAGAGGTTCCCCATGTGCATAATTTTTGGTGGTGATGGCAGCTATATGTCACCTTATAATCTTCACAATGATACGTTGTTGACCAACCTCCTTGGCCAGCATGTCCCAGAAACTGTTTGGAATCATTTGGTTGCTGGTTTGAATGCTCAGTTGAGGATGGTGAGGCATGGATCTATCCGTTCTCACCTAATTCCTGTGATTAATTGGATAAATAGCCATGCAAACCCTCAACTTGTGTTTCATGGAGTTAGAGTTGAGCTTGGGTGGTTTCAAGCAACAGCCTCTGGTTATTATCAGCTGGGAATATTGGTAGTGGTTGGGGATTATCCACTTCAAAGTCTGCACCAGTCTGATATGGGGGATAACGAGCTACCAAGGTCCAATGCTGCATGCACAAGAAAATGTTCAAAGCAGATGCAGCAAAATTGGCCATTTGTAGGTCATGCATTATCTGTTAAAAGGATAACTGGAGGAATTAATGGAGGTCTTATAAATCATACTACCTTGAGATCTTTGGATTATAAAAGGGATTTCCTCTTTCCACTCTCTCTCTTACTGCACAATACAAGACCTGTTGGTCGTCAGGACACCCTGCTGCTGCTGATAAGTTTCATGCTGTTAGAAGATCTTTCTGTCACCATTCTTATGTTACTTGAATTCTATTGGATATCTCTTGGGGCTTTCCTTGCTGTCTTGCTCATACTTCCTTTATCCCTTCTCTCTCCCTTCCTTGCTGGATTGAATGCCTTATTCAGTAAGGAACTTAGAAGAGCTTCACTAGCTCGTGTATATGCATTGTGGAATGCTACATCGCTTTCTAATATTGTGGTTGCTTTCATGTGCGGCATTTTACATTATGGATTTTCATTTTTCCAGCCACCTCACAAGTCAAATACATGGAACACTAGAAGGGATGATGATAAGTGGTGGCTTTTACCATCCATTCTTCTGCTATTCAAGTTAATGCAGGCGCTGTTTGTGGACTGGCACATAGCAAATTTAGAAATCCAAGATCATTCTCTATTTTTCCCAGATCCGGATGCGTTTTGGGCACACGAATCTGGAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1448

Amino Acids

156.47

Weight (kDa)

6.63

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 180, 1533, 3330, 3764, 3795
AasI GACNNNNNNGTC 1 cut(s) 3849
Acc65I GGTACC 2 cut(s) 24, 854
AccB1I GGYRCC 2 cut(s) 24, 854
AccB7I CCANNNNNTGG 1 cut(s) 2043
AccI GTMKAC 2 cut(s) 2648, 3031
AccII CGCG 1 cut(s) 3047
AccIII TCCGGA 1 cut(s) 4309
AciI CCGC 8 cut(s) 47, 509, 798, 897, 2139, 2559, 3079, 4116
AclWI GGATC 7 cut(s) 253, 303, 814, 2028, 2041, 3451, 4301
AcoI YGGCCR 5 cut(s) 568, 1459, 2045, 3367, 3701
AcsI RAATTY 8 cut(s) 283, 690, 1238, 1341, 1698, 2206, 3929, 4270
AcuI CTGAAG 6 cut(s) 66, 1275, 1365, 1493, 1547, 3089
AflIII ACRYGT 2 cut(s) 718, 1108
AhlI ACTAGT 1 cut(s) 521
AjiI CACGTC 1 cut(s) 719
AjnI CCWGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
AjuI GAANNNNNNNTTGG 2 cut(s) 2442, 2474
Alw26I GTCTC 1 cut(s) 1527
AlwI GGATC 7 cut(s) 253, 303, 814, 2028, 2041, 3451, 4301
AlwNI CAGNNNCTG 4 cut(s) 1064, 3386, 3557, 4246
Ama87I CYCGRG 2 cut(s) 2633, 3159
Aor13HI TCCGGA 1 cut(s) 4309
ApaI GGGCCC 1 cut(s) 2664
ApoI RAATTY 8 cut(s) 283, 690, 1238, 1341, 1698, 2206, 3929, 4270
AseI ATTAAT 2 cut(s) 3474, 3750
Asp700I GAANNNNTTC 8 cut(s) 156, 820, 1260, 1846, 2316, 3278, 3676, 4045
Asp718I GGTACC 2 cut(s) 24, 854
AspLEI GCGC 1 cut(s) 4246
AvaI CYCGRG 2 cut(s) 2633, 3159
AvaII GGWCC 8 cut(s) 229, 1155, 1714, 1982, 2240, 2882, 3063, 3651
BaeGI GKGCMC 2 cut(s) 2664, 4327
BalI TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
BamHI GGATCC 1 cut(s) 2033
BanI GGYRCC 2 cut(s) 24, 854
BanII GRGCYC 2 cut(s) 1895, 2664
BauI CACGAG 1 cut(s) 4056
BbsI GAAGAC 2 cut(s) 2798, 3134
BbvCI CCTCAGC 1 cut(s) 1977
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
BciVI GTATCC 2 cut(s) 1236, 1892
BclI TGATCA 1 cut(s) 1797
BcoDI GTCTC 1 cut(s) 1527
BcuI ACTAGT 1 cut(s) 521
BfaI CTAG 7 cut(s) 522, 1814, 2112, 2328, 3251, 4052, 4179
BfmI CTRYAG 2 cut(s) 435, 768
BfoI RGCGCY 1 cut(s) 4247
BfuI GTATCC 2 cut(s) 1236, 1892
BglI GCCNNNNNGGC 2 cut(s) 2424, 2460
BglII AGATCT 3 cut(s) 3155, 3782, 3898
Bme1390I CCNGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
Bme18I GGWCC 8 cut(s) 229, 1155, 1714, 1982, 2240, 2882, 3063, 3651
BmeT110I CYCGRG 2 cut(s) 2633, 3159
BmgBI CACGTC 1 cut(s) 719
BmrFI CCNGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
BmrI ACTGGG 2 cut(s) 1980, 2415
BmuI ACTGGG 2 cut(s) 1980, 2415
BpiI GAAGAC 2 cut(s) 2798, 3134
BpmI CTGGAG 4 cut(s) 344, 516, 1011, 3762
Bpu10I CCTNAGC 1 cut(s) 1977
BpuEI CTTGAG 1 cut(s) 3799
BsaAI YACGTR 1 cut(s) 630
BsaBI GATNNNNATC 1 cut(s) 2493
BsaWI WCCGGW 3 cut(s) 1654, 2320, 4309
BsaXI ACNNNNNCTCC 8 cut(s) 655, 685, 705, 735, 2630, 2660, 3510, 3540
Bse3DI GCAATG 2 cut(s) 1927, 1948
Bse8I GATNNNNATC 1 cut(s) 2493
BseAI TCCGGA 1 cut(s) 4309
BseBI CCWGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
BseJI GATNNNNATC 1 cut(s) 2493
BseMI GCAATG 2 cut(s) 1927, 1948
BseMII CTCAG 8 cut(s) 153, 1403, 1968, 2529, 2582, 2708, 2811, 3434
BseRI GAGGAG 3 cut(s) 816, 2651, 2913
BseSI GKGCMC 2 cut(s) 2664, 4327
BseYI CCCAGC 3 cut(s) 2225, 2363, 3571
BsgI GTGCAG 2 cut(s) 3599, 3815
Bsh1236I CGCG 1 cut(s) 3047
BshNI GGYRCC 2 cut(s) 24, 854
BsiHKCI CYCGRG 2 cut(s) 2633, 3159
BsiSI CCGG 3 cut(s) 1655, 2321, 4310
BslFI GGGAC 6 cut(s) 802, 1479, 1968, 2176, 2565, 3362
BsmAI GTCTC 1 cut(s) 1527
BsmFI GGGAC 6 cut(s) 802, 1479, 1968, 2176, 2565, 3362
BsmI GAATGC 6 cut(s) 39, 991, 2456, 3421, 4021, 4081
BsoBI CYCGRG 2 cut(s) 2633, 3159
Bsp120I GGGCCC 1 cut(s) 2660
Bsp1286I GDGCHC 3 cut(s) 1895, 2664, 4327
Bsp13I TCCGGA 1 cut(s) 4309
Bsp1407I TGTACA 3 cut(s) 826, 952, 3168
Bsp19I CCATGG 1 cut(s) 2903
BspACI CCGC 8 cut(s) 47, 509, 798, 897, 2139, 2559, 3079, 4116
BspCNI CTCAG 8 cut(s) 152, 1404, 1969, 2530, 2581, 2709, 2812, 3433
BspEI TCCGGA 1 cut(s) 4309
BspFNI CGCG 1 cut(s) 3047
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 7 cut(s) 253, 303, 814, 2028, 2041, 3451, 4301
BspQI GCTCTTC 1 cut(s) 4036
BspT107I GGYRCC 2 cut(s) 24, 854
BsrDI GCAATG 2 cut(s) 1927, 1948
BsrGI TGTACA 3 cut(s) 826, 952, 3168
BssNAI GTATAC 2 cut(s) 2649, 3032
BssSI CACGAG 1 cut(s) 4056
BssT1I CCWWGG 4 cut(s) 2903, 3229, 3364, 3647
Bst1107I GTATAC 2 cut(s) 2649, 3032
Bst2BI CACGAG 1 cut(s) 4056
Bst2UI CCWGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
Bst4CI ACNGT 6 cut(s) 952, 1193, 1543, 2786, 3075, 3388
Bst6I CTCTTC 6 cut(s) 111, 871, 1879, 2266, 3269, 4036
BstAPI GCANNNNNTGC 2 cut(s) 425, 2451
BstAUI TGTACA 3 cut(s) 826, 952, 3168
BstBAI YACGTR 1 cut(s) 630
BstC8I GCNNGC 4 cut(s) 1000, 3371, 3664, 4242
BstDSI CCRYGG 3 cut(s) 650, 1192, 2903
BstENI CCTNNNNNAGG 1 cut(s) 2819
BstFNI CGCG 1 cut(s) 3047
BstH2I RGCGCY 1 cut(s) 4247
BstHHI GCGC 1 cut(s) 4246
BstMAI GTCTC 1 cut(s) 1527
BstNI CCWGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
BstNSI RCATGY 7 cut(s) 56, 264, 833, 1112, 1632, 3377, 3666
BstSCI CCNGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
BstSFI CTRYAG 2 cut(s) 435, 768
BstSLI GKGCMC 2 cut(s) 2664, 4327
BstUI CGCG 1 cut(s) 3047
BstV2I GAAGAC 2 cut(s) 2798, 3134
BstX2I RGATCY 8 cut(s) 295, 806, 2033, 3155, 3443, 3782, 3898, 4306
BstXI CCANNNNNNTGG 1 cut(s) 1152
BstYI RGATCY 8 cut(s) 295, 806, 2033, 3155, 3443, 3782, 3898, 4306
BstZ17I GTATAC 2 cut(s) 2649, 3032
BsuI GTATCC 2 cut(s) 1236, 1892
BtgI CCRYGG 3 cut(s) 650, 1192, 2903
BtgZI GCGATG 3 cut(s) 2406, 3083, 4068
BtrI CACGTC 1 cut(s) 719
BtsI GCAGTG 2 cut(s) 1800, 2359
BtsIMutI CAGTG 8 cut(s) 540, 948, 1539, 1800, 2048, 2359, 2403, 3080
Cac8I GCNNGC 4 cut(s) 1000, 3371, 3664, 4242
CaiI CAGNNNCTG 4 cut(s) 1064, 3386, 3557, 4246
CfoI GCGC 1 cut(s) 4246
CseI GACGC 1 cut(s) 3236
DrdI GACNNNNNNGTC 1 cut(s) 3849
DseDI GACNNNNNNGTC 1 cut(s) 3849
EaeI YGGCCR 5 cut(s) 568, 1459, 2045, 3367, 3701
Eam1104I CTCTTC 6 cut(s) 111, 871, 1879, 2266, 3269, 4036
EarI CTCTTC 6 cut(s) 111, 871, 1879, 2266, 3269, 4036
EciI GGCGGA 2 cut(s) 813, 2154
Eco130I CCWWGG 4 cut(s) 2903, 3229, 3364, 3647
Eco24I GRGCYC 2 cut(s) 1895, 2664
Eco32I GATATC 2 cut(s) 63, 3942
Eco47I GGWCC 8 cut(s) 229, 1155, 1714, 1982, 2240, 2882, 3063, 3651
Eco57I CTGAAG 6 cut(s) 66, 1275, 1365, 1493, 1547, 3089
Eco88I CYCGRG 2 cut(s) 2633, 3159
EcoNI CCTNNNNNAGG 1 cut(s) 2819
EcoO109I RGGNCCY 7 cut(s) 229, 1714, 1982, 2425, 2660, 2661, 2882
EcoRI GAATTC 2 cut(s) 2206, 3929
EcoRII CCWGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
EcoRV GATATC 2 cut(s) 63, 3942
EcoT14I CCWWGG 4 cut(s) 2903, 3229, 3364, 3647
EcoT22I ATGCAT 3 cut(s) 2962, 3721, 4069
EcoT38I GRGCYC 2 cut(s) 1895, 2664
ErhI CCWWGG 4 cut(s) 2903, 3229, 3364, 3647
FaqI GGGAC 6 cut(s) 802, 1479, 1968, 2176, 2565, 3362
FauI CCCGC 2 cut(s) 40, 2552
FauNDI CATATG 2 cut(s) 1996, 3001
FbaI TGATCA 1 cut(s) 1797
FblI GTMKAC 2 cut(s) 2648, 3031
FriOI GRGCYC 2 cut(s) 1895, 2664
FspBI CTAG 7 cut(s) 522, 1814, 2112, 2328, 3251, 4052, 4179
GlaI GCGC 1 cut(s) 4245
GsaI CCCAGC 3 cut(s) 2229, 2367, 3575
GsuI CTGGAG 4 cut(s) 344, 516, 1011, 3762
HaeII RGCGCY 1 cut(s) 4247
HapII CCGG 3 cut(s) 1655, 2321, 4310
HgaI GACGC 1 cut(s) 3236
HhaI GCGC 1 cut(s) 4246
Hin6I GCGC 1 cut(s) 4244
HinP1I GCGC 1 cut(s) 4244
HincII GTYRAC 3 cut(s) 636, 1479, 3354
HindII GTYRAC 3 cut(s) 636, 1479, 3354
HindIII AAGCTT 3 cut(s) 725, 2057, 2095
HpaII CCGG 3 cut(s) 1655, 2321, 4310
Hpy166II GTNNAC 6 cut(s) 636, 1479, 2649, 3032, 3354, 4255
Hpy8I GTNNAC 6 cut(s) 636, 1479, 2649, 3032, 3354, 4255
HpyCH4III ACNGT 6 cut(s) 952, 1193, 1543, 2786, 3075, 3388
HpyCH4IV ACGT 4 cut(s) 629, 718, 3347, 4340
HpySE526I ACGT 4 cut(s) 629, 718, 3347, 4340
HspAI GCGC 1 cut(s) 4244
KflI GGGWCCC 1 cut(s) 1982
Kpn2I TCCGGA 1 cut(s) 4309
KpnI GGTACC 2 cut(s) 28, 858
Ksp22I TGATCA 1 cut(s) 1797
LguI GCTCTTC 1 cut(s) 4036
LmnI GCTCC 7 cut(s) 992, 1573, 1856, 1973, 2345, 2638, 3188
MaeI CTAG 7 cut(s) 522, 1814, 2112, 2328, 3251, 4052, 4179
MaeII ACGT 4 cut(s) 629, 718, 3347, 4340
MfeI CAATTG 3 cut(s) 345, 357, 2772
MflI RGATCY 8 cut(s) 295, 806, 2033, 3155, 3443, 3782, 3898, 4306
MhlI GDGCHC 3 cut(s) 1895, 2664, 4327
MlsI TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
MluNI TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
MlyI GAGTC 3 cut(s) 1314, 1630, 2560
MmeI TCCRAC 4 cut(s) 223, 1018, 1990, 3166
Mox20I TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
Mph1103I ATGCAT 3 cut(s) 2962, 3721, 4069
MroI TCCGGA 1 cut(s) 4309
MroXI GAANNNNTTC 8 cut(s) 156, 820, 1260, 1846, 2316, 3278, 3676, 4045
MscI TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
MslI CAYNNNNRTG 3 cut(s) 1491, 2549, 3917
Msp20I TGGCCA 4 cut(s) 1461, 2047, 3369, 3703
MspA1I CMGCKG 1 cut(s) 3571
MspI CCGG 3 cut(s) 1655, 2321, 4310
MspR9I CCNGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
MunI CAATTG 3 cut(s) 345, 357, 2772
Mva1269I GAATGC 6 cut(s) 39, 991, 2456, 3421, 4021, 4081
MvaI CCWGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
MvnI CGCG 1 cut(s) 3047
NcoI CCATGG 1 cut(s) 2903
NdeI CATATG 2 cut(s) 1996, 3001
NmeAIII GCCGAG 1 cut(s) 596
NmuCI GTSAC 6 cut(s) 184, 946, 1104, 2563, 3321, 3907
NsiI ATGCAT 3 cut(s) 2962, 3721, 4069
NspI RCATGY 7 cut(s) 56, 264, 833, 1112, 1632, 3377, 3666
PaeI GCATGC 1 cut(s) 3666
PaeR7I CTCGAG 2 cut(s) 2633, 3159
PciI ACATGT 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 4036
PctI GAATGC 6 cut(s) 39, 991, 2456, 3421, 4021, 4081
PdmI GAANNNNTTC 8 cut(s) 156, 820, 1260, 1846, 2316, 3278, 3676, 4045
PfeI GAWTC 7 cut(s) 212, 328, 1274, 1399, 2730, 3394, 4331
PflMI CCANNNNNTGG 1 cut(s) 2043
PfoI TCCNGGA 1 cut(s) 3065
PleI GAGTC 3 cut(s) 1313, 1629, 2560
PpsI GAGTC 3 cut(s) 1313, 1629, 2560
Ppu21I YACGTR 1 cut(s) 630
PpuMI RGGWCCY 4 cut(s) 229, 1714, 1982, 2882
PscI ACATGT 1 cut(s) 1108
PshBI ATTAAT 2 cut(s) 3474, 3750
PsiI TTATAA 5 cut(s) 180, 1533, 3330, 3764, 3795
Psp5II RGGWCCY 4 cut(s) 229, 1714, 1982, 2882
Psp6I CCWGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
PspFI CCCAGC 3 cut(s) 2225, 2363, 3571
PspGI CCWGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
PspOMI GGGCCC 1 cut(s) 2660
PspPPI RGGWCCY 4 cut(s) 229, 1714, 1982, 2882
PsrI GAACNNNNNNTAC 2 cut(s) 2494, 2526
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 4 cut(s) 1064, 3386, 3557, 4246
PsuI RGATCY 8 cut(s) 295, 806, 2033, 3155, 3443, 3782, 3898, 4306
PvuII CAGCTG 1 cut(s) 3571
RseI CAYNNNNRTG 3 cut(s) 1491, 2549, 3917
SapI GCTCTTC 1 cut(s) 4036
SchI GAGTC 3 cut(s) 1314, 1630, 2560
ScrFI CCNGG 7 cut(s) 233, 323, 981, 1463, 2665, 3061, 3067
SduI GDGCHC 3 cut(s) 1895, 2664, 4327
SfcI CTRYAG 2 cut(s) 435, 768
Sfr274I CTCGAG 2 cut(s) 2633, 3159
SinI GGWCC 8 cut(s) 229, 1155, 1714, 1982, 2240, 2882, 3063, 3651
SlaI CTCGAG 2 cut(s) 2633, 3159
SmiMI CAYNNNNRTG 3 cut(s) 1491, 2549, 3917
SmlI CTYRAG 3 cut(s) 2633, 3159, 3778
SmoI CTYRAG 3 cut(s) 2633, 3159, 3778
SpeI ACTAGT 1 cut(s) 521
SphI GCATGC 1 cut(s) 3666
SsiI CCGC 8 cut(s) 47, 509, 798, 897, 2139, 2559, 3079, 4116
SspI AATATT 2 cut(s) 3579, 4096
SspMI CTAG 7 cut(s) 522, 1814, 2112, 2328, 3251, 4052, 4179
StyD4I CCNGG 7 cut(s) 231, 321, 979, 1461, 2663, 3059, 3065
StyI CCWWGG 4 cut(s) 2903, 3229, 3364, 3647
TaaI ACNGT 6 cut(s) 952, 1193, 1543, 2786, 3075, 3388
TaiI ACGT 4 cut(s) 632, 721, 3350, 4343
TaqI TCGA 4 cut(s) 1080, 2634, 2866, 3160
TatI WGTACW 5 cut(s) 90, 826, 952, 2871, 3168
TauI GCSGC 2 cut(s) 3082, 4119
TfiI GAWTC 7 cut(s) 212, 328, 1274, 1399, 2730, 3394, 4331
TscAI CASTG 8 cut(s) 540, 955, 1546, 1807, 2048, 2359, 2410, 3080
TseFI GTSAC 6 cut(s) 184, 946, 1104, 2563, 3321, 3907
Tsp45I GTSAC 6 cut(s) 184, 946, 1104, 2563, 3321, 3907
TspGWI ACGGA 2 cut(s) 1980, 3440
TspRI CASTG 8 cut(s) 540, 955, 1546, 1807, 2048, 2359, 2410, 3080
Van91I CCANNNNNTGG 1 cut(s) 2043
VpaK11BI GGWCC 8 cut(s) 229, 1155, 1714, 1982, 2240, 2882, 3063, 3651
VspI ATTAAT 2 cut(s) 3474, 3750
XagI CCTNNNNNAGG 1 cut(s) 2819
XapI RAATTY 8 cut(s) 283, 690, 1238, 1341, 1698, 2206, 3929, 4270
XbaI TCTAGA 1 cut(s) 3250
XceI RCATGY 7 cut(s) 56, 264, 833, 1112, 1632, 3377, 3666
XcmI CCANNNNNNNNNTGG 2 cut(s) 460, 3387
XhoI CTCGAG 2 cut(s) 2633, 3159
XmiI GTMKAC 2 cut(s) 2648, 3031
XmnI GAANNNNTTC 8 cut(s) 156, 820, 1260, 1846, 2316, 3278, 3676, 4045
XspI CTAG 7 cut(s) 522, 1814, 2112, 2328, 3251, 4052, 4179
Zsp2I ATGCAT 3 cut(s) 2962, 3721, 4069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.