Rroxscaffold_3G00250990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001588
Physical Location & Seq
Forward (+)
44848104 .. 44863119
15016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250990.1

Sequence Viewer

Length: 4254 bp
ATGCCATTCTTATCTCTCTGCCTCTGCTCTGCTCCCCTCTGCTCTCTCGCCTTTCGCTTCCAGGCCAAAGCGTTTGATTCTTTGGTACTGTTCTTCGTTTCGTTTGATTTCAAGAATTATGGTTTGGGCAATGAGAGTAGAAGCTGCAAAGTTCCTGAGGATGGGCAGTACAGAAGTACAGGCACCATGTCAGAAGAGTGGATGAAAGGTTTGGGGTCACATATAAGTGATGGCTTTGAAAGCAGCACCTCGACTTATGTGGTAACAATGTTATCATCTCCATTAAATGAACCAGTATCTTGTGAGGACCTGGAAGGTGTTGGATCATTCAATACCACATGCTTGTTAAACTCAAACTTCTCTTTGAATTCTGATCTTCACATACGTGGTACTGGAAACCTGGAGATACTCCCTCATTTGCTGGTTCTGTGGTTTTTTGCGGCTGCCAATCTGACTATGGGGTATAATTCTTCTATAAATACAACATCTTTGGGTGGACTACCGCCTTCTCAAACTAGTGGAACTCCAGTGGGCTATGATGGAGGTGGTGGAGGGCATGGTGGCCGAGGAGCTTCTTGCTTGAAAAATAATCAGACGAGCTTTTGGGGTGGTGATGTTTATACTTGGTCAAAATTGTCCGAGCCATGGAGTTATGGGAGCAAGGGTGGAGGCACATCTACTAAAATTCCATTTGGAGGAAATGGTGGAGGACGTGTCAAGCTTCAAGTGAAGGATATGTTATATCTAAATGGATCTGTAACTGCAGAAGGAGGAGACGGCGGAACAACTGGTGGAGGAGGATCTGGTGGAAGTATTTTTATACATGCTGTTAAGTTGAAGGGATATGGTACAATAAGTGCTGCTGGTGGAAGAGGATGGGGTGGAGGTGGTGGTGGAAGAATATCACTTGATTGTTACAGCATACAAGAAGATGTAAAAGTCACTGTCCATGGTGGTTTAAGTATTGGCTGTCCTGGGAATTCTGGAGCAGCTGGCACATACTTTAATGCAGATCTGCTTAGTTTAAGAGTTGGAAATGACAATATCACAACAGAAACTGAAACCCCATTGCTTGATTTTTCTACCAGCCCATTATGGTCACATGTTTTTGTGGAGAATAGCGCAAAAGTGTTGGTTCCACTGCTGTGGACCAGAGTTCAGGTGAGAGGCCAAGTTAGTTTATACCGTGGAGGCAGCATCATCTTTGGACTGTCTGAGTTTCCAGTTTCCGAATTTGAACTTGTTGCTGAAGAGCTTCTGATGAGTGATTCCATCATAAAGGTGTTTGGTGCATTTAGAGTTGCTGTCAAGATGTTACTCATGTGGAACTCCAGAATTGAAATAAATGGCGGTGGAAATACCATTGTTACTTCTTCAGTTCTTGAAGTTAGGAATCTCATTGAAATGAGGGTTGGTGCTGGTTCTTTACTTCAGGCTCCAATGGATGATGATGCAAGCCGAAAAGTGGTAACAAAATCCCTATGCGAGAGTGAGACATGCCCGTTGGATTTGATTACCCCACCAGATGATTGCCATGTCAATTATACACTCTCCTTTTCACTTCAAATTTGTCGCGTAGAGGATCTTCTTGTTCATGGCGTTGTCAAGGGAAGTGTTGTTCACATCCACAGGGCTAGGACTATTATTATTGCTACTGATGGCCGGATCAGTGCATCTGAACTTGGTTGCAGTAAAGGTATTGGAAAGGGAAACTATTTAAATGGAGCTGGTAGTGGTGCTGGACATGGGGGAAGAGGGGGCTCGGGTTATTATAATGGGAGGGTGAGCAATGGCGGTAATGAATACGGTGATGCTAATTTCCCGTGTGAATTAGGGAGTGGAGCTGAGGGTGCCAATCAGTCATATGGAAATGTGGTAGGAGGGGGAATGATTGTGATGGGATCCATCCAGTGGCCACTCTTAAGGCTTGATGTCTATGGATCTTTGAGTGCTGATGGTCAAAGCTTTGATAAGGCAGCTAGAAACAGTAATGGTTCTATAATTGGCGGGCTTGGTGGAGGTTCAGGTGGGACAATCCTTCTTTTCCTTCAGGAGCTGAGGTTACTTGCACAGAATTCATCGTTATCTGTAGTGGGAGGCAAAGGTGGTCCACGAGGTGGGGGTGGGGGTGGGGGTGGAAGAGTTCATTTTCATTGGTCCAAGATAGAATTCAGGGATGAATATACTCCATTTGCGAGTATAAGTGGCTCCATTAACAGAAGTGGAGGTAATGGAGATGATGGAGGTAGTCATGGATATGATGGAACTATCACTGGGAAGAAATGCCCTAAGGGCCTTTATGGCACCTTCTGCAAGGAATGCCCTGTTGGCACATATAAAAATGTTGAGGGCTCTGATGCTCATCTTTGTACTCCTTGTTCTGTTGACCTTCTTCCACGACGTGCTGAATTCATTTATGTTCGAGGCGGGGTGACTCGTCCATCGTGTCCTTACAAATGCATATCTGACAAATATAGGATGCCAAAATGCTATACTCCTCTTGAGGAGCTTTTGTATACCTTTGGGGGGCCCTGGCCTTTTGCAATTTTATTGTCATGCATTTTGGTGCTTCTGGGTCTGTTATTAAGTACTTTGAGAATTAAACTTGTTGGATCATGCTCTCACGATAGAGCTGGTTCAGTTGAAGACCATAATCGTCATCGTTTTCCCTCTCTTCTTTCCTTATCTGAGGTGCGAGGAACCCGAGTTGAAGAAACTCAAAGCCATGTTCATCGCATGTACTTCATGGGTCCTAATACATTCAGAGAACCTTGGCATCTTCCTTATTCTCCTCCAGATGCAATCATTGAAATTGTGTATGAAGATGCATTTAACAGATTCATTGATGAGATCAATTCTGTGGCTGCTTATGACTGGTGGGAAGGGTCTGTTCATAGTATACTTTCAATTCTCGCATATCCTTGTTCCTGGTCCTGGAAACAGTGGCGGCAAAGAAATAAAATTCATCGCCTTCAGGAATATGTAAAGTCTGAATATGACCATTCGTGTCTCCGCTCTTGCAGATCTCGAGCCTTGTACAAAGGGATGAAGGTTTCTGAGCTTTCTGATGTAAGAGGGCTTGAGGTTGGAGCAACGCCAGACTTGATGGTAGCATATATTGATTTTTTCCTTGGTGGTGATGAGAAGCGTCTGGAATTGGTATCAATCATCCAGAAGAGGTTCCCCATGTGCATAATCTTTGGTGGTGATGGAAGCTATATGTCACCTTATAATCTTCACAGTGATTCATTGTTGACCAATCTCCTTGGCCAGCATGTCCCAGAAACTGTTTGGAATCATTTGGTGGCTGGTTTGAATGCTCAGTTGAGGATGGTGAGGCATGGATCTATTCGTACTCACCTAATTCCTGTTATTAATTGGATAAATAGCCACGCAAACCCTCAACTTGAGTTCCATGGGGTTAAAATTGAGCTTTTATGGTTTCAAGCAACAGCTTCTGGTTATTATCAGCTGGGGATATTGGTAGTAGTTGGAGATTATCCTCTTCAAAGTGTGCACCAGTTGAATACGTGGGATAGAAGTGATAATGAGCTGCCAAGGTCAAATTCTGCATGTGCAAGAAAGTGTCTAAAGCAAATGCAGCAAAGTTGGCCGTATGCAGGTCATGCATTATCAATTAAAAGGATAACTGGAGGAATAAATGGAGGCCTTATAAATCATACTACCTTGAGATCTATGGAGTACAAAAAGGATTTCCTCTTTCCACTTTCTCTTCTACTGAACAATACAAGACCTATTGGCCGTCAGGACACCCTGCTGCTGCTGATAAGTGTCATGCTTTTAGCAGACCTATCTGTCACCCTTCTCATGTTGCTTCAATACTATTGGATTTCTCTTGGGGCTTTTCTTGCTGTCTTGCTTATACTTCCTCTATCTCTACTTTCTCCCTTCCTTGCCGGATTGAGTGCCTTATTCAGTAAGGAAAATAGAAGAGCTTCACTTGCTCGGGTATATGCATTGTGGAATGCTACGTCCCTATCTAACATTGTGGTTGCATTCATATGTGGCATTTTCCATTATAGCTTTTCCTTTTTCCAGCCACCTGACCAGTCAAATACATGGAACACTAGGAGGGAGGATGATAAATGGTGGCTTTTGCCAACTGTTCTTCTGCTGTTCAAGTTAATACAAGCCCGTTTTGTAGACTGGCACATAGCGAATCTAGAAATTCAAGATTATTCTTTATTTTGCCCAGATCCAGATGCCTTTTGGGCACTTGAGGCTGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1417

Amino Acids

154.11

Weight (kDa)

6.77

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 757 - 831 9.4e-35 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1773, 3231, 3674
AasI GACNNNNNNGTC 1 cut(s) 3815
Acc36I ACCTGC 1 cut(s) 3611
AccB1I GGYRCC 3 cut(s) 182, 1850, 2303
AccB7I CCANNNNNTGG 2 cut(s) 1911, 2117
AccBSI CCGCTC 1 cut(s) 3015
AccI GTMKAC 3 cut(s) 2516, 2899, 4164
AccII CGCG 1 cut(s) 1575
AciI CCGC 9 cut(s) 440, 503, 780, 1350, 1794, 2007, 2427, 2947, 3013
AcoI YGGCCR 6 cut(s) 562, 1660, 1913, 3268, 3611, 3760
AcuI CTGAAG 5 cut(s) 1269, 1359, 1415, 2033, 2957
AdeI CACNNNGTG 2 cut(s) 2117, 2402
AfiI CCNNNNNNNGG 9 cut(s) 161, 645, 695, 1465, 1911, 2117, 2526, 2934, 3620
AflII CTTAAG 1 cut(s) 1921
AflIII ACRYGT 2 cut(s) 712, 1102
AhlI ACTAGT 1 cut(s) 515
AjiI CACGTC 2 cut(s) 713, 2402
AjnI CCWGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
AleI CACNNNNGTG 2 cut(s) 384, 1144
AloI GAACNNNNNNTCC 2 cut(s) 1602, 1634
Alw21I GWGCWC 1 cut(s) 3519
Alw26I GTCTC 3 cut(s) 768, 1487, 3014
Alw44I GTGCAC 1 cut(s) 3515
AlwNI CAGNNNCTG 4 cut(s) 1058, 2056, 3287, 3458
Ama87I CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
ApaI GGGCCC 1 cut(s) 2532
ApaLI GTGCAC 1 cut(s) 3515
AseI ATTAAT 1 cut(s) 3375
Asp700I GAANNNNTTC 3 cut(s) 1254, 3179, 3955
AspLEI GCGC 1 cut(s) 1124
AspS9I GGNCC 9 cut(s) 307, 1149, 2108, 2157, 2293, 2528, 2529, 2750, 2931
AvaI CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
AvaII GGWCC 6 cut(s) 307, 1149, 2108, 2157, 2750, 2931
AxyI CCTNAGG 2 cut(s) 156, 2289
BaeGI GKGCMC 3 cut(s) 2532, 3519, 4237
BalI TGGCCA 2 cut(s) 1915, 3270
BamHI GGATCC 1 cut(s) 1901
BanI GGYRCC 3 cut(s) 182, 1850, 2303
BanII GRGCYC 3 cut(s) 1763, 2354, 2532
BarI GAAGNNNNNNTAC 2 cut(s) 1569, 1601
BauI CACGAG 1 cut(s) 2112
BbsI GAAGAC 1 cut(s) 2652
Bbv12I GWGCWC 1 cut(s) 3519
BbvCI CCTCAGC 2 cut(s) 1845, 2057
BceAI ACGGC 3 cut(s) 793, 3598, 3747
BciT130I CCWGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
BcoDI GTCTC 3 cut(s) 768, 1487, 3014
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 5 cut(s) 516, 1635, 1980, 4089, 4184
BfmI CTRYAG 2 cut(s) 762, 2088
BfrI CTTAAG 1 cut(s) 1921
BfuAI ACCTGC 1 cut(s) 3611
BglI GCCNNNNNGGC 4 cut(s) 2292, 2301, 2328, 4232
BglII AGATCT 3 cut(s) 1012, 3023, 3692
BmcAI AGTACT 1 cut(s) 2590
Bme1390I CCNGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
Bme18I GGWCC 6 cut(s) 307, 1149, 2108, 2157, 2750, 2931
BmeT110I CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
BmgBI CACGTC 2 cut(s) 713, 2402
BmgT120I GGNCC 9 cut(s) 307, 1149, 2108, 2157, 2293, 2528, 2529, 2750, 2931
BmrFI CCNGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
BmrI ACTGGG 1 cut(s) 2283
BmuI ACTGGG 1 cut(s) 2283
BpiI GAAGAC 1 cut(s) 2652
BpmI CTGGAG 6 cut(s) 422, 510, 1005, 1315, 2778, 3672
Bpu10I CCTNAGC 2 cut(s) 1845, 2057
BpuEI CTTGAG 4 cut(s) 2522, 3101, 3428, 3709
BsaAI YACGTR 2 cut(s) 386, 3531
BsaBI GATNNNNATC 1 cut(s) 2361
BsaXI ACNNNNNCTCC 8 cut(s) 649, 679, 699, 729, 2045, 2075, 2498, 2528
Bsc4I CCNNNNNNNGG 9 cut(s) 161, 645, 695, 1465, 1911, 2117, 2526, 2934, 3620
Bse21I CCTNAGG 2 cut(s) 156, 2289
Bse3DI GCAATG 3 cut(s) 136, 1067, 1795
Bse8I GATNNNNATC 1 cut(s) 2361
BseBI CCWGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
BseJI GATNNNNATC 1 cut(s) 2361
BseLI CCNNNNNNNGG 9 cut(s) 161, 645, 695, 1465, 1911, 2117, 2526, 2934, 3620
BseMI GCAATG 3 cut(s) 136, 1067, 1795
BseMII CTCAG 7 cut(s) 147, 1206, 1836, 2048, 2679, 3048, 3335
BseRI GAGGAG 6 cut(s) 582, 786, 810, 2487, 2519, 2781
BseSI GKGCMC 3 cut(s) 2532, 3519, 4237
BseYI CCCAGC 1 cut(s) 3472
Bsh1236I CGCG 1 cut(s) 1575
BshNI GGYRCC 3 cut(s) 182, 1850, 2303
BsiHKAI GWGCWC 1 cut(s) 3519
BsiHKCI CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
BsiSI CCGG 2 cut(s) 1663, 3918
BslFI GGGAC 3 cut(s) 2044, 3263, 3979
BslI CCNNNNNNNGG 9 cut(s) 161, 645, 695, 1465, 1911, 2117, 2526, 2934, 3620
BsmAI GTCTC 3 cut(s) 768, 1487, 3014
BsmBI CGTCTC 1 cut(s) 768
BsmFI GGGAC 3 cut(s) 2044, 3263, 3979
BsmI GAATGC 4 cut(s) 2324, 3322, 3991, 4016
BsoBI CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
Bsp120I GGGCCC 1 cut(s) 2528
Bsp1286I GDGCHC 5 cut(s) 1763, 2354, 2532, 3519, 4237
Bsp1407I TGTACA 1 cut(s) 3036
Bsp19I CCATGG 3 cut(s) 644, 949, 3415
BspACI CCGC 9 cut(s) 440, 503, 780, 1350, 1794, 2007, 2427, 2947, 3013
BspCNI CTCAG 7 cut(s) 148, 1207, 1837, 2049, 2680, 3049, 3334
BspFNI CGCG 1 cut(s) 1575
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 3611
BspQI GCTCTTC 2 cut(s) 1245, 3946
BspT107I GGYRCC 3 cut(s) 182, 1850, 2303
BspTI CTTAAG 1 cut(s) 1921
BsrBI CCGCTC 1 cut(s) 3015
BsrDI GCAATG 3 cut(s) 136, 1067, 1795
BsrGI TGTACA 1 cut(s) 3036
BssNAI GTATAC 2 cut(s) 2517, 2900
BssSI CACGAG 1 cut(s) 2112
BssT1I CCWWGG 7 cut(s) 644, 949, 2771, 3130, 3265, 3415, 3557
Bst1107I GTATAC 2 cut(s) 2517, 2900
Bst2BI CACGAG 1 cut(s) 2112
Bst2UI CCWGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
BstAFI CTTAAG 1 cut(s) 1921
BstAPI GCANNNNNTGC 3 cut(s) 2310, 2319, 3626
BstAUI TGTACA 1 cut(s) 3036
BstBAI YACGTR 2 cut(s) 386, 3531
BstC8I GCNNGC 5 cut(s) 994, 1456, 2009, 3272, 4246
BstDEI CTNAG 9 cut(s) 156, 1019, 1215, 1845, 2057, 2289, 2688, 3057, 3321
BstDSI CCRYGG 4 cut(s) 644, 949, 1186, 3415
BstFNI CGCG 1 cut(s) 1575
BstHHI GCGC 1 cut(s) 1124
BstMAI GTCTC 3 cut(s) 768, 1487, 3014
BstNI CCWGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
BstNSI RCATGY 7 cut(s) 342, 827, 1106, 1500, 2740, 3278, 3576
BstSCI CCNGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
BstSFI CTRYAG 2 cut(s) 762, 2088
BstSLI GKGCMC 3 cut(s) 2532, 3519, 4237
BstUI CGCG 1 cut(s) 1575
BstV2I GAAGAC 1 cut(s) 2652
BstXI CCANNNNNNTGG 1 cut(s) 1146
BstZ17I GTATAC 2 cut(s) 2517, 2900
Bsu36I CCTNAGG 2 cut(s) 156, 2289
BtgI CCRYGG 4 cut(s) 644, 949, 1186, 3415
BtgZI GCGATG 2 cut(s) 2717, 2951
BtrI CACGTC 2 cut(s) 713, 2402
BtsI GCAGTG 1 cut(s) 1139
BtsIMutI CAGTG 8 cut(s) 534, 942, 1139, 1675, 1916, 2271, 2948, 3247
BveI ACCTGC 1 cut(s) 3611
Cac8I GCNNGC 5 cut(s) 994, 1456, 2009, 3272, 4246
CaiI CAGNNNCTG 4 cut(s) 1058, 2056, 3287, 3458
CfoI GCGC 1 cut(s) 1124
Cfr13I GGNCC 9 cut(s) 307, 1149, 2108, 2157, 2293, 2528, 2529, 2750, 2931
CseI GACGC 1 cut(s) 3137
DdeI CTNAG 9 cut(s) 156, 1019, 1215, 1845, 2057, 2289, 2688, 3057, 3321
DraI TTTAAA 1 cut(s) 1719
DraIII CACNNNGTG 2 cut(s) 2117, 2402
DrdI GACNNNNNNGTC 1 cut(s) 3815
DseDI GACNNNNNNGTC 1 cut(s) 3815
EaeI YGGCCR 6 cut(s) 562, 1660, 1913, 3268, 3611, 3760
EciI GGCGGA 1 cut(s) 795
Eco130I CCWWGG 7 cut(s) 644, 949, 2771, 3130, 3265, 3415, 3557
Eco147I AGGCCT 1 cut(s) 3669
Eco24I GRGCYC 3 cut(s) 1763, 2354, 2532
Eco47I GGWCC 6 cut(s) 307, 1149, 2108, 2157, 2750, 2931
Eco57I CTGAAG 5 cut(s) 1269, 1359, 1415, 2033, 2957
Eco81I CCTNAGG 2 cut(s) 156, 2289
Eco88I CYCGRG 4 cut(s) 1762, 2703, 3027, 3966
EcoO109I RGGNCCY 5 cut(s) 307, 2293, 2528, 2529, 2750
EcoRI GAATTC 5 cut(s) 367, 979, 2074, 2168, 2408
EcoRII CCWGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
EcoT14I CCWWGG 7 cut(s) 644, 949, 2771, 3130, 3265, 3415, 3557
EcoT22I ATGCAT 5 cut(s) 2462, 2561, 2830, 3631, 3979
EcoT38I GRGCYC 3 cut(s) 1763, 2354, 2532
ErhI CCWWGG 7 cut(s) 644, 949, 2771, 3130, 3265, 3415, 3557
Esp3I CGTCTC 1 cut(s) 768
FaqI GGGAC 3 cut(s) 2044, 3263, 3979
FauI CCCGC 2 cut(s) 2000, 2420
FauNDI CATATG 2 cut(s) 1864, 4022
FblI GTMKAC 3 cut(s) 2516, 2899, 4164
FriOI GRGCYC 3 cut(s) 1763, 2354, 2532
FspBI CTAG 5 cut(s) 516, 1635, 1980, 4089, 4184
GlaI GCGC 1 cut(s) 1123
GsaI CCCAGC 1 cut(s) 3476
GsuI CTGGAG 6 cut(s) 422, 510, 1005, 1315, 2778, 3672
HapII CCGG 2 cut(s) 1663, 3918
HgaI GACGC 1 cut(s) 3137
HhaI GCGC 1 cut(s) 1124
Hin6I GCGC 1 cut(s) 1122
HinP1I GCGC 1 cut(s) 1122
HincII GTYRAC 2 cut(s) 2386, 3255
HindII GTYRAC 2 cut(s) 2386, 3255
HindIII AAGCTT 2 cut(s) 719, 1963
HinfI GANTC 8 cut(s) 77, 1268, 1393, 2434, 2838, 3245, 3295, 4180
HpaII CCGG 2 cut(s) 1663, 3918
Hpy99I CGWCG 1 cut(s) 2403
HpyCH4IV ACGT 5 cut(s) 385, 712, 2401, 3530, 3992
HpyF3I CTNAG 9 cut(s) 156, 1019, 1215, 1845, 2057, 2289, 2688, 3057, 3321
HpySE526I ACGT 5 cut(s) 385, 712, 2401, 3530, 3992
HspAI GCGC 1 cut(s) 1122
LguI GCTCTTC 2 cut(s) 1245, 3946
MaeI CTAG 5 cut(s) 516, 1635, 1980, 4089, 4184
MaeII ACGT 5 cut(s) 385, 712, 2401, 3530, 3992
MbiI CCGCTC 1 cut(s) 3015
MhlI GDGCHC 5 cut(s) 1763, 2354, 2532, 3519, 4237
MlsI TGGCCA 2 cut(s) 1915, 3270
MluNI TGGCCA 2 cut(s) 1915, 3270
MlyI GAGTC 1 cut(s) 2428
MmeI TCCRAC 6 cut(s) 301, 1012, 1485, 2590, 3067, 3472
Mox20I TGGCCA 2 cut(s) 1915, 3270
Mph1103I ATGCAT 5 cut(s) 2462, 2561, 2830, 3631, 3979
MroXI GAANNNNTTC 3 cut(s) 1254, 3179, 3955
MscI TGGCCA 2 cut(s) 1915, 3270
MslI CAYNNNNRTG 8 cut(s) 225, 384, 1144, 1280, 1403, 2256, 2564, 4021
Msp20I TGGCCA 2 cut(s) 1915, 3270
MspA1I CMGCKG 2 cut(s) 992, 3472
MspCI CTTAAG 1 cut(s) 1921
MspI CCGG 2 cut(s) 1663, 3918
MspR9I CCNGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
Mva1269I GAATGC 4 cut(s) 2324, 3322, 3991, 4016
MvaI CCWGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
MvnI CGCG 1 cut(s) 1575
NcoI CCATGG 3 cut(s) 644, 949, 3415
NdeI CATATG 2 cut(s) 1864, 4022
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 6 cut(s) 216, 940, 1098, 2431, 3222, 3817
NsiI ATGCAT 5 cut(s) 2462, 2561, 2830, 3631, 3979
NspI RCATGY 7 cut(s) 342, 827, 1106, 1500, 2740, 3278, 3576
OliI CACNNNNGTG 2 cut(s) 384, 1144
PaeR7I CTCGAG 1 cut(s) 3027
PceI AGGCCT 1 cut(s) 3669
PciI ACATGT 1 cut(s) 1102
PciSI GCTCTTC 2 cut(s) 1245, 3946
PctI GAATGC 4 cut(s) 2324, 3322, 3991, 4016
PdmI GAANNNNTTC 3 cut(s) 1254, 3179, 3955
PfeI GAWTC 7 cut(s) 77, 1268, 1393, 2838, 3245, 3295, 4180
PflMI CCANNNNNTGG 2 cut(s) 1911, 2117
PfoI TCCNGGA 1 cut(s) 2933
PleI GAGTC 1 cut(s) 2428
PpsI GAGTC 1 cut(s) 2428
Ppu21I YACGTR 2 cut(s) 386, 3531
PpuMI RGGWCCY 2 cut(s) 307, 2750
PscI ACATGT 1 cut(s) 1102
PshBI ATTAAT 1 cut(s) 3375
PsiI TTATAA 3 cut(s) 1773, 3231, 3674
Psp5II RGGWCCY 2 cut(s) 307, 2750
Psp6I CCWGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
PspFI CCCAGC 1 cut(s) 3472
PspGI CCWGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
PspOMI GGGCCC 1 cut(s) 2528
PspPI GGNCC 9 cut(s) 307, 1149, 2108, 2157, 2293, 2528, 2529, 2750, 2931
PspPPI RGGWCCY 2 cut(s) 307, 2750
PsrI GAACNNNNNNTAC 2 cut(s) 2362, 2394
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 4 cut(s) 1058, 2056, 3287, 3458
PvuII CAGCTG 2 cut(s) 992, 3472
RseI CAYNNNNRTG 8 cut(s) 225, 384, 1144, 1280, 1403, 2256, 2564, 4021
SapI GCTCTTC 2 cut(s) 1245, 3946
Sau96I GGNCC 9 cut(s) 307, 1149, 2108, 2157, 2293, 2528, 2529, 2750, 2931
ScaI AGTACT 1 cut(s) 2590
SchI GAGTC 1 cut(s) 2428
ScrFI CCNGG 7 cut(s) 62, 311, 401, 975, 2533, 2929, 2935
SduI GDGCHC 5 cut(s) 1763, 2354, 2532, 3519, 4237
SfcI CTRYAG 2 cut(s) 762, 2088
Sfr274I CTCGAG 1 cut(s) 3027
SinI GGWCC 6 cut(s) 307, 1149, 2108, 2157, 2750, 2931
SlaI CTCGAG 1 cut(s) 3027
SmiI ATTTAAAT 1 cut(s) 1719
SmiMI CAYNNNNRTG 8 cut(s) 225, 384, 1144, 1280, 1403, 2256, 2564, 4021
SmlI CTYRAG 7 cut(s) 1921, 2501, 3027, 3080, 3407, 3688, 4238
SmoI CTYRAG 7 cut(s) 1921, 2501, 3027, 3080, 3407, 3688, 4238
SpeI ACTAGT 1 cut(s) 515
SseBI AGGCCT 1 cut(s) 3669
SsiI CCGC 9 cut(s) 440, 503, 780, 1350, 1794, 2007, 2427, 2947, 3013
SspMI CTAG 5 cut(s) 516, 1635, 1980, 4089, 4184
StuI AGGCCT 1 cut(s) 3669
StyD4I CCNGG 7 cut(s) 60, 309, 399, 973, 2531, 2927, 2933
StyI CCWWGG 7 cut(s) 644, 949, 2771, 3130, 3265, 3415, 3557
SwaI ATTTAAAT 1 cut(s) 1719
TaiI ACGT 5 cut(s) 388, 715, 2404, 3533, 3995
TaqI TCGA 3 cut(s) 251, 2422, 3028
TatI WGTACW 7 cut(s) 168, 176, 2369, 2588, 2739, 3036, 3702
TauI GCSGC 2 cut(s) 443, 2950
TfiI GAWTC 7 cut(s) 77, 1268, 1393, 2838, 3245, 3295, 4180
TscAI CASTG 8 cut(s) 534, 949, 1146, 1675, 1916, 2278, 2948, 3247
TseFI GTSAC 6 cut(s) 216, 940, 1098, 2431, 3222, 3817
Tsp45I GTSAC 6 cut(s) 216, 940, 1098, 2431, 3222, 3817
TspRI CASTG 8 cut(s) 534, 949, 1146, 1675, 1916, 2278, 2948, 3247
Van91I CCANNNNNTGG 2 cut(s) 1911, 2117
Vha464I CTTAAG 1 cut(s) 1921
VneI GTGCAC 1 cut(s) 3515
VpaK11BI GGWCC 6 cut(s) 307, 1149, 2108, 2157, 2750, 2931
VspI ATTAAT 1 cut(s) 3375
XbaI TCTAGA 1 cut(s) 4183
XceI RCATGY 7 cut(s) 342, 827, 1106, 1500, 2740, 3278, 3576
XcmI CCANNNNNNNNNTGG 3 cut(s) 454, 3288, 4227
XhoI CTCGAG 1 cut(s) 3027
XmiI GTMKAC 3 cut(s) 2516, 2899, 4164
XmnI GAANNNNTTC 3 cut(s) 1254, 3179, 3955
XspI CTAG 5 cut(s) 516, 1635, 1980, 4089, 4184
ZrmI AGTACT 1 cut(s) 2590
Zsp2I ATGCAT 5 cut(s) 2462, 2561, 2830, 3631, 3979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.