Rorug07G0100300

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001588
Physical Location & Seq
Forward (+)
7954612 .. 7957867
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0100300.1

Sequence Viewer

Length: 1056 bp
ATGGGAGGTAATCCAATAAATGGAGGGTTAATTCTCTCTGTTGGATTTGTGCCCGATTTAAAAGTGGATGTCATCCCACTTCCAAGATCAAAGACTCAACCCAATATGCCATTGATGGAGTACTTTATGCTGGAGCTTAGGAGTTACACTGCTTCAGTTTCAGGTTCTCTCGACCCAGCTTGTATCACAATGTTCGGAGATCATCGTGTTGATATAAGACCCATTGTTGAGAAACTAGATCTTGTCATGCCTGAGGAGACTGCCGTTGTAGGAGACGCAAAAGGCTCCTTTCTGTTCCGCCGCAGGGACCAAACCCAAAATTACAGTGCTGATCTGTTCTTCTTTGATGCTGCTGCTCTAGTATTTGCCAGGGACCAAAACAAGTCTGCTGAGTTAGGGGAAACTCGGTTCCATGTGGCGGTGGCCGACGGCATAATTCCATTTGGTCCTGAGCTCAGGAGCGTTTCAGTGTTTGAGGATTTCTTTGCTCAATCTTGGATTTCGGGCGTAGTGGAAGGAGCAGAAGAAGGGTATCCCATGCTTGATTGCGTCACTGTTTTACAACAGCTTATAAATCAGTTGGACTTGGGAAACCCAAATTTATACATTGAGGTCAGGAAAGGTAGACAATACTCCACACAATTCAGAACAACCCTGGCTTGTCATGAACTTTTGGGGGGTAACTACGAGTCCATAATCGTCAAGGGTTGTGGCATACAACCTGGGGATAGATTCATATTCCTCCACTCAGACGCCGGAAGTGCACTGACTCGTGTTGACGATGCTCGTCACAGTTTGGAAGCATTGGATGCTAATCAAGAGGTGTTTGGAGGCTTGATCTTCTCTAGCTACCACCGCGACAGGTCCTTTTTCGGAGCCTCTCATGTGGATATAACTCCCTTTACTGACAAGCTCAGTGGAGTTCCCATGGCTGGTATCTCCTGCACAAGAGAAATTGGGCGTGGGGCGTTACTTGGTCCAGGACTAGGGTATTGCCTCCATGCACATAGCTGTGTGTACCTAGCTATGTCTTATGTTCCTCCTATGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

351

Amino Acids

38.53

Weight (kDa)

5.11

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 20
AccI GTMKAC 1 cut(s) 625
AccII CGCG 1 cut(s) 858
AciI CCGC 4 cut(s) 298, 301, 419, 856
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 753
AfaI GTAC 2 cut(s) 122, 1019
AfiI CCNNNNNNNGG 5 cut(s) 20, 304, 418, 932, 986
AjnI CCWGG 4 cut(s) 368, 654, 721, 979
AloI GAACNNNNNNTCC 2 cut(s) 392, 424
AluBI AGCT 8 cut(s) 136, 179, 454, 568, 849, 913, 1011, 1025
AluI AGCT 8 cut(s) 136, 179, 454, 568, 849, 913, 1011, 1025
Alw21I GWGCWC 2 cut(s) 456, 766
Alw26I GTCTC 2 cut(s) 251, 267
Alw44I GTGCAC 1 cut(s) 762
AoxI GGCC 1 cut(s) 423
ApaLI GTGCAC 1 cut(s) 762
ApeKI GCWGC 2 cut(s) 350, 353
ApoI RAATTY 1 cut(s) 598
AspS9I GGNCC 5 cut(s) 307, 373, 446, 864, 977
AvaII GGWCC 5 cut(s) 307, 373, 446, 864, 977
AxyI CCTNAGG 1 cut(s) 252
BaeGI GKGCMC 2 cut(s) 54, 766
BanII GRGCYC 1 cut(s) 456
BauI CACGAG 1 cut(s) 771
Bbv12I GWGCWC 2 cut(s) 456, 766
BbvI GCAGC 2 cut(s) 337, 340
BccI CCATC 1 cut(s) 109
BceAI ACGGC 2 cut(s) 248, 445
BciT130I CCWGG 4 cut(s) 370, 656, 723, 981
BciVI GTATCC 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 251, 267
BfaI CTAG 5 cut(s) 236, 359, 846, 986, 1022
BfuI GTATCC 1 cut(s) 543
BglII AGATCT 1 cut(s) 238
BisI GCNGC 3 cut(s) 301, 351, 354
BlsI GCNGC 3 cut(s) 302, 352, 355
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 4 cut(s) 370, 656, 723, 981
Bme18I GGWCC 5 cut(s) 307, 373, 446, 864, 977
BmgT120I GGNCC 5 cut(s) 307, 373, 446, 864, 977
BmiI GGNNCC 5 cut(s) 286, 308, 374, 410, 877
BmrFI CCNGG 4 cut(s) 370, 656, 723, 981
BmsI GCATC 3 cut(s) 337, 772, 799
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 3 cut(s) 137, 450, 455
BsaBI GATNNNNATC 1 cut(s) 813
BsaHI GRCGYC 1 cut(s) 753
BsaJI CCNNGG 4 cut(s) 369, 654, 722, 927
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 304, 418, 932, 986
Bse21I CCTNAGG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 813
BseBI CCWGG 4 cut(s) 370, 656, 723, 981
BseDI CCNNGG 4 cut(s) 369, 654, 722, 927
BseGI GGATG 3 cut(s) 72, 73, 814
BseJI GATNNNNATC 1 cut(s) 813
BseLI CCNNNNNNNGG 5 cut(s) 20, 304, 418, 932, 986
BseMII CTCAG 6 cut(s) 243, 381, 441, 469, 762, 928
BseRI GAGGAG 1 cut(s) 269
BseSI GKGCMC 2 cut(s) 54, 766
BseXI GCAGC 2 cut(s) 337, 340
BseYI CCCAGC 1 cut(s) 175
BsgI GTGCAG 1 cut(s) 928
Bsh1236I CGCG 1 cut(s) 858
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 2 cut(s) 456, 766
BsiSI CCGG 1 cut(s) 756
BslFI GGGAC 2 cut(s) 320, 386
BslI CCNNNNNNNGG 5 cut(s) 20, 304, 418, 932, 986
BsmAI GTCTC 2 cut(s) 251, 267
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 2 cut(s) 320, 386
BsnI GGCC 1 cut(s) 425
Bsp1286I GDGCHC 3 cut(s) 54, 456, 766
Bsp143I GATC 5 cut(s) 86, 199, 238, 331, 837
Bsp19I CCATGG 1 cut(s) 927
BspACI CCGC 4 cut(s) 298, 301, 419, 856
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 6 cut(s) 244, 382, 442, 468, 761, 927
BspFNI CGCG 1 cut(s) 858
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 5 cut(s) 286, 308, 374, 410, 877
BssECI CCNNGG 4 cut(s) 369, 654, 722, 927
BssMI GATC 5 cut(s) 86, 199, 238, 331, 837
BssNI GRCGYC 1 cut(s) 753
BssSI CACGAG 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 771
Bst2UI CCWGG 4 cut(s) 370, 656, 723, 981
Bst4CI ACNGT 3 cut(s) 326, 556, 794
BstACI GRCGYC 1 cut(s) 753
BstAPI GCANNNNNTGC 1 cut(s) 809
BstDEI CTNAG 7 cut(s) 137, 252, 390, 450, 455, 748, 914
BstDSI CCRYGG 1 cut(s) 927
BstF5I GGATG 3 cut(s) 72, 73, 814
BstFNI CGCG 1 cut(s) 858
BstKTI GATC 5 cut(s) 89, 202, 241, 334, 840
BstMAI GTCTC 2 cut(s) 251, 267
BstMBI GATC 5 cut(s) 86, 199, 238, 331, 837
BstMWI GCNNNNNNNGC 3 cut(s) 761, 809, 855
BstNI CCWGG 4 cut(s) 370, 656, 723, 981
BstSCI CCNGG 4 cut(s) 368, 654, 721, 979
BstSLI GKGCMC 2 cut(s) 54, 766
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 2 cut(s) 337, 340
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu36I CCTNAGG 1 cut(s) 252
BsuI GTATCC 1 cut(s) 543
BsuRI GGCC 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 927
BtsCI GGATG 3 cut(s) 72, 73, 814
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 6 cut(s) 147, 331, 474, 552, 764, 922
CciI TCATGA 1 cut(s) 664
Cfr13I GGNCC 5 cut(s) 307, 373, 446, 864, 977
CseI GACGC 3 cut(s) 284, 538, 761
Csp6I GTAC 2 cut(s) 121, 1018
CspCI CAANNNNNGTGG 4 cut(s) 691, 726, 898, 933
CviAII CATG 7 cut(s) 247, 413, 538, 665, 884, 928, 1001
CviQI GTAC 2 cut(s) 121, 1018
DdeI CTNAG 7 cut(s) 137, 252, 390, 450, 455, 748, 914
DpnI GATC 5 cut(s) 88, 201, 240, 333, 839
DpnII GATC 5 cut(s) 86, 199, 238, 331, 837
DraI TTTAAA 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 423
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 5 cut(s) 307, 373, 446, 864, 977
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 454
EcoO109I RGGNCCY 1 cut(s) 864
EcoRII CCWGG 4 cut(s) 368, 654, 721, 979
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 927
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 7 cut(s) 250, 416, 541, 668, 887, 931, 1004
FaqI GGGAC 2 cut(s) 320, 386
FatI CATG 7 cut(s) 246, 412, 537, 664, 883, 927, 1000
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 3 cut(s) 301, 351, 354
FokI GGATG 3 cut(s) 59, 80, 821
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 301, 351, 354
FspBI CTAG 5 cut(s) 236, 359, 846, 986, 1022
GluI GCNGC 3 cut(s) 301, 351, 354
GsaI CCCAGC 1 cut(s) 179
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 425
HapII CCGG 1 cut(s) 756
HgaI GACGC 3 cut(s) 284, 538, 761
Hin1I GRCGYC 1 cut(s) 753
Hin1II CATG 7 cut(s) 250, 416, 541, 668, 887, 931, 1004
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 4 cut(s) 94, 689, 732, 769
HpaII CCGG 1 cut(s) 756
Hpy166II GTNNAC 4 cut(s) 626, 764, 778, 1018
Hpy188I TCNGA 4 cut(s) 197, 647, 751, 875
Hpy188III TCNNGA 6 cut(s) 170, 449, 457, 616, 665, 818
Hpy8I GTNNAC 4 cut(s) 626, 764, 778, 1018
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 509, 521
HpyCH4III ACNGT 3 cut(s) 326, 556, 794
HpyCH4V TGCA 3 cut(s) 764, 945, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 761, 809, 855
HpyF3I CTNAG 7 cut(s) 137, 252, 390, 450, 455, 748, 914
Hsp92I GRCGYC 1 cut(s) 753
Hsp92II CATG 7 cut(s) 250, 416, 541, 668, 887, 931, 1004
Kzo9I GATC 5 cut(s) 86, 199, 238, 331, 837
LmnI GCTCC 5 cut(s) 133, 290, 459, 518, 875
Lsp1109I GCAGC 2 cut(s) 337, 340
LweI GCATC 3 cut(s) 337, 772, 799
MaeI CTAG 5 cut(s) 236, 359, 846, 986, 1022
MaeIII GTNAC 5 cut(s) 143, 550, 680, 788, 969
MalI GATC 5 cut(s) 88, 201, 240, 333, 839
MboI GATC 5 cut(s) 86, 199, 238, 331, 837
MboII GAAGA 3 cut(s) 331, 536, 832
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 3 cut(s) 54, 456, 766
MluCI AATT 6 cut(s) 30, 319, 435, 598, 641, 954
MlyI GAGTC 3 cut(s) 88, 698, 763
MmeI TCCRAC 2 cut(s) 22, 561
MseI TTAA 2 cut(s) 29, 59
MslI CAYNNNNRTG 1 cut(s) 1011
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 4 cut(s) 370, 656, 723, 981
MvaI CCWGG 4 cut(s) 370, 656, 723, 981
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 3 cut(s) 761, 809, 855
NcoI CCATGG 1 cut(s) 927
NdeII GATC 5 cut(s) 86, 199, 238, 331, 837
NlaIII CATG 7 cut(s) 250, 416, 541, 668, 887, 931, 1004
NlaIV GGNNCC 5 cut(s) 286, 308, 374, 410, 877
NmuCI GTSAC 2 cut(s) 550, 788
PagI TCATGA 1 cut(s) 664
PfeI GAWTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 979
PkrI GCNGC 3 cut(s) 302, 352, 355
PleI GAGTC 3 cut(s) 88, 697, 763
PpsI GAGTC 3 cut(s) 88, 697, 763
PpuMI RGGWCCY 1 cut(s) 864
PsiI TTATAA 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 864
Psp6I CCWGG 4 cut(s) 368, 654, 721, 979
PspFI CCCAGC 1 cut(s) 175
PspGI CCWGG 4 cut(s) 368, 654, 721, 979
PspN4I GGNNCC 5 cut(s) 286, 308, 374, 410, 877
PspPI GGNCC 5 cut(s) 307, 373, 446, 864, 977
PspPPI RGGWCCY 1 cut(s) 864
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 2 cut(s) 122, 1019
RsaNI GTAC 2 cut(s) 121, 1018
RseI CAYNNNNRTG 1 cut(s) 1011
SacI GAGCTC 1 cut(s) 456
SaqAI TTAA 2 cut(s) 29, 59
SatI GCNGC 3 cut(s) 301, 351, 354
Sau3AI GATC 5 cut(s) 86, 199, 238, 331, 837
Sau96I GGNCC 5 cut(s) 307, 373, 446, 864, 977
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 3 cut(s) 88, 698, 763
ScrFI CCNGG 4 cut(s) 370, 656, 723, 981
SduI GDGCHC 3 cut(s) 54, 456, 766
SfaNI GCATC 3 cut(s) 337, 772, 799
SinI GGWCC 5 cut(s) 307, 373, 446, 864, 977
SmiMI CAYNNNNRTG 1 cut(s) 1011
Sse9I AATT 6 cut(s) 30, 319, 435, 598, 641, 954
SsiI CCGC 4 cut(s) 298, 301, 419, 856
SspMI CTAG 5 cut(s) 236, 359, 846, 986, 1022
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 4 cut(s) 368, 654, 721, 979
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 3 cut(s) 326, 556, 794
TaqI TCGA 1 cut(s) 171
TasI AATT 6 cut(s) 30, 319, 435, 598, 641, 954
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 2 cut(s) 29, 59
Tru9I TTAA 2 cut(s) 29, 59
TscAI CASTG 6 cut(s) 154, 331, 474, 559, 771, 922
TseFI GTSAC 2 cut(s) 550, 788
TseI GCWGC 2 cut(s) 350, 353
Tsp45I GTSAC 2 cut(s) 550, 788
TspDTI ATGAA 2 cut(s) 681, 724
TspRI CASTG 6 cut(s) 154, 331, 474, 559, 771, 922
Van91I CCANNNNNTGG 1 cut(s) 20
VneI GTGCAC 1 cut(s) 762
VpaK11BI GGWCC 5 cut(s) 307, 373, 446, 864, 977
XapI RAATTY 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 625
XspI CTAG 5 cut(s) 236, 359, 846, 986, 1022
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.