Prupe.6G270700_v2.0.a1

anion transporter 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010728
Physical Location & Seq
Reverse (-)
25679964 .. 25683708
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G270700.1

Sequence Viewer

Length: 1581 bp
ATGGCGGCTGTATCTACCACCACTACATCGCAGCTGCATATTTCATACCCTGCTCGTTTATTCAAAACCCAATCAACTCTGATCAATACAGAGAAAACCCAGTTGGGGTTTAAGCCCAGAGGCAGAGTCCAAGCAAAATGCGAGACGGGGATTGCTTCTTCTGCAGGTTTCGGATTGGGAAACAGAGGAAGAAGAAGGAAAACAGAGGGGTTTCAGAGGGGGAAGCCATGGGTGGTGACGTGTACGGCCGAGGGCATAGAGAGAGGAATGTTGATGGGAAACAGAGGAGGAGGATCAGGAGAGGCTGCTGCTGCTAAGAAAGCAATCAGTGTGCCAGAGAGGTTCAAGGTGGTGGCTTTGTTGGCGTGTGTGATGTGCTTGTGTAATGCCGATAGAGTCGTCATGTCTGTGGCTATTGTTCCACTCGCAGCTAAAAATGGCTGGACCAGTTCTTTCTTGGGCGTCGTTCAGTCATCTTTTCTATGGGGATACATATTCTCATCAGTGATTGGAGGAGCCTTGGTGGACAAGTATGGAGGAAAGAGAGTGATGGCATGGGGTGTGGCCATGTGGTCTCTGGCTACTCTCCTCACCCCATGGGCTGCCAACCATTCCACAACCAGCCTCTTGGCCGTTCGTGCCTTCTTTGGACTGGCTGAAGGTGTGGCCTTGCCATCCATGAGCACCCTCTTGTCCAGGTGGTTTCCAACCCAAGAAAGAGCAAGTGCAGTTGGAATCTCTATGGCTGGTTTTCACATTGGCAATGTCATAGGCTTACTGCTAACTCCAGTTATGTTATCATCAATTGGAATTTCTGGCCCCTTTATTCTCTTCTCTTCCATTGGACTTCTCTGGCTGACAATCTGGGCATATACAGTCACAAATGATCCAGGAGAGAGTTATTGCATCAGCAAATCAGAGCTCCGATTGATCCAAGCTGGAAAATCTGATTCTCCAGTAAACAGTGGCAAGCTCCCACCTCTGAGGCTCCTCTTATCAAAATTACCAACATGGACCATTATTTTGGCCAATATAACTAACAATTGGGGATACTTTGTTCTACTCTCGTGGATGCCTGTTTATTTTAAGACTGTATTTGGTGTAAACTTGAAGCAAGCAGCATGGTTTAGTGCTGTTCCATGGGGAACAATGGCAGTTTCCAGCTACATTGCTGGTGCAGCATCAGATTCATTAATCAAGGCAGGCTACTCTTTGACTTCAGTTCGAAAGATCATGCAGTCAATTGGTTTCATTGGACCTGGAGTGTCATTGCTCTGCCTAAACTATGCGAACACACCCACGGATGCAGCGGTACTCCTCACAGCAGCCTTGTGCTTCAGCTCTTTCAGCCAAGCAGGCTTTCTCCTTAATATACAAGATATCGCCCCTCAATACGCTGGATTTCTCCACGGGATTTCAAATTCAGCAGGGACATTTGCAGCCATAGTCAGCACAATAGGAACTGGTTATTTTGTACAATGGTTAGGATCATTTCAGGCATTCCTAACTGTCACTGCGGCGCTCTATTTCGCGACAGCAATCTTTTGGAACCTATTTGCTACTGGAGAGCGTGTCTTCTAG

Protein Analysis

527

Amino Acids

56.19

Weight (kDa)

9.81

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010728)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38060 AT2G38060
fragaria_vesca FvH4_6g12020 FvH4_6g12020 FvH4_6g12020 FvH4_6g12020
malus_domestica MD04G1146900.v1.1
prunus_persica Prupe.6G270700_v2.0.a1
pyrus_communis pycom04g13270
rosa_chinensis RchiOBHm_Chr3g0463851
rosa_laevigata RLG00000024750
rosa_multiflora Rmu_sc0004796.1_g000002
rosa_roxburghii Rroxscaffold_6G00416250
rosa_rugosa Rorug03G0068400 Rorug03G0068500
rosa_samantha Rh3AG125600 Rh3BG129700 Rh3CG132200 Rh3DG130800
rosa_wichuraiana Rw3G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 155
AccII CGCG 1 cut(s) 1532
AciI CCGC 3 cut(s) 5, 1310, 1517
AclWI GGATC 4 cut(s) 301, 881, 925, 1495
AcoI YGGCCR 4 cut(s) 246, 564, 630, 1026
AcsI RAATTY 2 cut(s) 810, 1420
AcuI CTGAAG 3 cut(s) 678, 1203, 1321
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 3 cut(s) 244, 1314, 1476
AfiI CCNNNNNNNGG 1 cut(s) 105
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 4 cut(s) 64, 346, 1111, 1419
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 3 cut(s) 695, 889, 1258
AjuI GAANNNNNNNTTGG 4 cut(s) 86, 118, 1344, 1376
AloI GAACNNNNNNTCC 2 cut(s) 1041, 1073
AluBI AGCT 7 cut(s) 34, 431, 922, 938, 973, 1164, 1341
AluI AGCT 7 cut(s) 34, 431, 922, 938, 973, 1164, 1341
Alw21I GWGCWC 2 cut(s) 686, 924
Alw26I GTCTC 2 cut(s) 137, 579
AlwI GGATC 4 cut(s) 301, 881, 925, 1495
AoxI GGCC 6 cut(s) 246, 564, 630, 666, 817, 1026
ApoI RAATTY 2 cut(s) 810, 1420
ArsI GACNNNNNNTTYG 2 cut(s) 1006, 1038
AseI ATTAAT 1 cut(s) 1193
AspLEI GCGC 1 cut(s) 1522
AspS9I GGNCC 4 cut(s) 444, 818, 1014, 1256
AsuHPI GGTGA 2 cut(s) 247, 583
AsuII TTCGAA 1 cut(s) 1225
AvaII GGWCC 3 cut(s) 444, 1014, 1256
BalI TGGCCA 2 cut(s) 566, 1028
BanII GRGCYC 1 cut(s) 924
BauI CACGAG 1 cut(s) 1066
BbsI GAAGAC 1 cut(s) 1567
Bbv12I GWGCWC 2 cut(s) 686, 924
BccI CCATC 3 cut(s) 268, 544, 682
BceAI ACGGC 2 cut(s) 261, 617
BciT130I CCWGG 3 cut(s) 697, 891, 1260
BciVI GTATCC 2 cut(s) 482, 1043
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 137, 579
BfaI CTAG 1 cut(s) 1579
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 1523
BfuAI ACCTGC 1 cut(s) 155
BfuI GTATCC 2 cut(s) 482, 1043
BglI GCCNNNNNGGC 1 cut(s) 1356
Bme1390I CCNGG 3 cut(s) 697, 891, 1260
Bme18I GGWCC 3 cut(s) 444, 1014, 1256
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 4 cut(s) 444, 818, 1014, 1256
BmiI GGNNCC 4 cut(s) 517, 820, 989, 1550
BmrFI CCNGG 3 cut(s) 697, 891, 1260
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 4 cut(s) 915, 1062, 1190, 1294
BmuI ACTGGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 1567
BpmI CTGGAG 3 cut(s) 771, 939, 1281
Bpu14I TTCGAA 1 cut(s) 1225
BsaHI GRCGYC 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 7 cut(s) 227, 249, 519, 596, 1139, 1299, 1408
BsaXI ACNNNNNCTCC 3 cut(s) 507, 537, 1557
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 7 cut(s) 100, 447, 657, 788, 956, 1468, 1567
Bse3DI GCAATG 3 cut(s) 769, 1167, 1268
BseBI CCWGG 3 cut(s) 697, 891, 1260
BseDI CCNNGG 7 cut(s) 227, 249, 519, 596, 1139, 1299, 1408
BseGI GGATG 3 cut(s) 674, 1077, 1309
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 3 cut(s) 769, 1167, 1268
BseMII CTCAG 1 cut(s) 974
BseNI ACTGG 7 cut(s) 100, 447, 657, 788, 956, 1468, 1567
BseRI GAGGAG 6 cut(s) 300, 303, 528, 578, 980, 1307
BseX3I CGGCCG 1 cut(s) 246
BsgI GTGCAG 2 cut(s) 747, 1197
Bsh1236I CGCG 1 cut(s) 1532
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 6 cut(s) 248, 566, 632, 668, 819, 1028
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 2 cut(s) 686, 924
BslFI GGGAC 1 cut(s) 1444
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 2 cut(s) 137, 579
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 1444
BsmI GAATGC 1 cut(s) 1499
BsnI GGCC 6 cut(s) 248, 566, 632, 668, 819, 1028
Bso31I GGTCTC 1 cut(s) 579
Bsp119I TTCGAA 1 cut(s) 1225
Bsp1286I GDGCHC 2 cut(s) 686, 924
Bsp1407I TGTACA 1 cut(s) 1474
Bsp143I GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
Bsp19I CCATGG 3 cut(s) 227, 596, 1139
Bsp68I TCGCGA 1 cut(s) 1532
BspACI CCGC 3 cut(s) 5, 1310, 1517
BspANI GGCC 6 cut(s) 248, 566, 632, 668, 819, 1028
BspCNI CTCAG 1 cut(s) 975
BspFNI CGCG 1 cut(s) 1532
BspLI GGNNCC 4 cut(s) 517, 820, 989, 1550
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 4 cut(s) 301, 881, 925, 1495
BspT104I TTCGAA 1 cut(s) 1225
BspTNI GGTCTC 1 cut(s) 579
BsrDI GCAATG 3 cut(s) 769, 1167, 1268
BsrGI TGTACA 1 cut(s) 1474
BsrI ACTGG 7 cut(s) 100, 447, 657, 788, 956, 1468, 1567
BssECI CCNNGG 7 cut(s) 227, 249, 519, 596, 1139, 1299, 1408
BssMI GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 1066
BssT1I CCWWGG 4 cut(s) 227, 519, 596, 1139
Bst2BI CACGAG 1 cut(s) 1066
Bst2UI CCWGG 3 cut(s) 697, 891, 1260
Bst4CI ACNGT 4 cut(s) 877, 965, 1093, 1510
Bst6I CTCTTC 2 cut(s) 836, 841
BstACI GRCGYC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 1474
BstBI TTCGAA 1 cut(s) 1225
BstC8I GCNNGC 4 cut(s) 971, 1116, 1204, 1357
BstDEI CTNAG 2 cut(s) 315, 983
BstDSI CCRYGG 5 cut(s) 227, 596, 1139, 1299, 1408
BstF5I GGATG 3 cut(s) 674, 1077, 1309
BstFNI CGCG 1 cut(s) 1532
BstH2I RGCGCY 1 cut(s) 1523
BstHHI GCGC 1 cut(s) 1522
BstKTI GATC 6 cut(s) 84, 296, 889, 933, 1233, 1490
BstMAI GTCTC 2 cut(s) 137, 579
BstMBI GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 8 cut(s) 161, 311, 320, 362, 638, 1178, 1347, 1356
BstNI CCWGG 3 cut(s) 697, 891, 1260
BstSCI CCNGG 3 cut(s) 695, 889, 1258
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 1 cut(s) 1532
BstV2I GAAGAC 1 cut(s) 1567
BstXI CCANNNNNNTGG 2 cut(s) 628, 1024
BstZI CGGCCG 1 cut(s) 246
BsuI GTATCC 2 cut(s) 482, 1043
BsuRI GGCC 6 cut(s) 248, 566, 632, 668, 819, 1028
BtgI CCRYGG 5 cut(s) 227, 596, 1139, 1299, 1408
BtgZI GCGATG 1 cut(s) 12
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 3 cut(s) 674, 1077, 1309
BtsI GCAGTG 1 cut(s) 1512
BtsIMutI CAGTG 4 cut(s) 334, 510, 970, 1512
BtuMI TCGCGA 1 cut(s) 1532
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 4 cut(s) 971, 1116, 1204, 1357
CfoI GCGC 1 cut(s) 1522
Cfr13I GGNCC 4 cut(s) 444, 818, 1014, 1256
CseI GACGC 1 cut(s) 451
Csp6I GTAC 3 cut(s) 243, 1313, 1475
CviQI GTAC 3 cut(s) 243, 1313, 1475
DdeI CTNAG 2 cut(s) 315, 983
DpnI GATC 6 cut(s) 83, 295, 888, 932, 1232, 1489
DpnII GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
EaeI YGGCCR 4 cut(s) 246, 564, 630, 1026
EagI CGGCCG 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 836, 841
EarI CTCTTC 2 cut(s) 836, 841
Ecl136II GAGCTC 1 cut(s) 922
EclXI CGGCCG 1 cut(s) 246
Eco130I CCWWGG 4 cut(s) 227, 519, 596, 1139
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 579
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 3 cut(s) 444, 1014, 1256
Eco52I CGGCCG 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 3 cut(s) 678, 1203, 1321
EcoICRI GAGCTC 1 cut(s) 922
EcoRII CCWGG 3 cut(s) 695, 889, 1258
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 4 cut(s) 227, 519, 596, 1139
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 4 cut(s) 227, 519, 596, 1139
Esp3I CGTCTC 1 cut(s) 137
FaqI GGGAC 1 cut(s) 1444
FbaI TGATCA 1 cut(s) 81
FokI GGATG 3 cut(s) 661, 1084, 1316
FriOI GRGCYC 1 cut(s) 924
FspBI CTAG 1 cut(s) 1579
GlaI GCGC 1 cut(s) 1521
GsuI CTGGAG 3 cut(s) 771, 939, 1281
HaeII RGCGCY 1 cut(s) 1523
HaeIII GGCC 6 cut(s) 248, 566, 632, 668, 819, 1028
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 1522
Hin1I GRCGYC 1 cut(s) 462
Hin6I GCGC 1 cut(s) 1520
HinP1I GCGC 1 cut(s) 1520
HinfI GANTC 5 cut(s) 126, 396, 735, 950, 1187
HphI GGTGA 2 cut(s) 247, 583
Hpy166II GTNNAC 4 cut(s) 243, 526, 961, 1105
Hpy188I TCNGA 8 cut(s) 81, 173, 216, 919, 926, 949, 984, 1186
Hpy188III TCNNGA 2 cut(s) 297, 1531
Hpy8I GTNNAC 4 cut(s) 243, 526, 961, 1105
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 3 cut(s) 189, 652, 653
HpyCH4III ACNGT 4 cut(s) 877, 965, 1093, 1510
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 8 cut(s) 37, 164, 728, 906, 1178, 1237, 1307, 1439
HpyF10VI GCNNNNNNNGC 8 cut(s) 161, 311, 320, 362, 638, 1178, 1347, 1356
HpyF3I CTNAG 2 cut(s) 315, 983
HpySE526I ACGT 1 cut(s) 239
Hsp92I GRCGYC 1 cut(s) 462
HspAI GCGC 1 cut(s) 1520
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
LmnI GCTCC 4 cut(s) 515, 927, 978, 993
LweI GCATC 4 cut(s) 915, 1062, 1190, 1294
MaeI CTAG 1 cut(s) 1579
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 3 cut(s) 235, 877, 1510
MalI GATC 6 cut(s) 83, 295, 888, 932, 1232, 1489
MboI GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
MboII GAAGA 6 cut(s) 150, 201, 204, 823, 828, 1567
MfeI CAATTG 3 cut(s) 804, 1042, 1242
MhlI GDGCHC 2 cut(s) 686, 924
MlsI TGGCCA 2 cut(s) 566, 1028
MluCI AATT 6 cut(s) 804, 810, 1001, 1042, 1242, 1420
MluNI TGGCCA 2 cut(s) 566, 1028
MlyI GAGTC 2 cut(s) 135, 405
MmeI TCCRAC 2 cut(s) 712, 731
Mox20I TGGCCA 2 cut(s) 566, 1028
MscI TGGCCA 2 cut(s) 566, 1028
MseI TTAA 4 cut(s) 111, 1086, 1193, 1368
MslI CAYNNNNRTG 1 cut(s) 407
Msp20I TGGCCA 2 cut(s) 566, 1028
MspA1I CMGCKG 2 cut(s) 34, 1310
MspR9I CCNGG 3 cut(s) 697, 891, 1260
MunI CAATTG 3 cut(s) 804, 1042, 1242
Mva1269I GAATGC 1 cut(s) 1499
MvaI CCWGG 3 cut(s) 697, 891, 1260
MvnI CGCG 1 cut(s) 1532
MwoI GCNNNNNNNGC 8 cut(s) 161, 311, 320, 362, 638, 1178, 1347, 1356
NcoI CCATGG 3 cut(s) 227, 596, 1139
NdeII GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
NlaIV GGNNCC 4 cut(s) 517, 820, 989, 1550
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 3 cut(s) 235, 877, 1510
NruI TCGCGA 1 cut(s) 1532
NspV TTCGAA 1 cut(s) 1225
PctI GAATGC 1 cut(s) 1499
PfeI GAWTC 3 cut(s) 735, 950, 1187
PfoI TCCNGGA 1 cut(s) 889
PleI GAGTC 2 cut(s) 134, 404
PpsI GAGTC 2 cut(s) 134, 404
PshBI ATTAAT 1 cut(s) 1193
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 3 cut(s) 695, 889, 1258
PspGI CCWGG 3 cut(s) 695, 889, 1258
PspN4I GGNNCC 4 cut(s) 517, 820, 989, 1550
PspPI GGNCC 4 cut(s) 444, 818, 1014, 1256
PstI CTGCAG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 34
RruI TCGCGA 1 cut(s) 1532
RsaI GTAC 3 cut(s) 244, 1314, 1476
RsaNI GTAC 3 cut(s) 243, 1313, 1475
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 4 cut(s) 111, 1086, 1193, 1368
Sau3AI GATC 6 cut(s) 81, 293, 886, 930, 1230, 1487
Sau96I GGNCC 4 cut(s) 444, 818, 1014, 1256
SchI GAGTC 2 cut(s) 135, 405
ScrFI CCNGG 3 cut(s) 697, 891, 1260
SduI GDGCHC 2 cut(s) 686, 924
SfaNI GCATC 4 cut(s) 915, 1062, 1190, 1294
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 1225
SinI GGWCC 3 cut(s) 444, 1014, 1256
SmiMI CAYNNNNRTG 1 cut(s) 407
Sse9I AATT 6 cut(s) 804, 810, 1001, 1042, 1242, 1420
SsiI CCGC 3 cut(s) 5, 1310, 1517
SspMI CTAG 1 cut(s) 1579
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 3 cut(s) 695, 889, 1258
StyI CCWWGG 4 cut(s) 227, 519, 596, 1139
TaaI ACNGT 4 cut(s) 877, 965, 1093, 1510
TaiI ACGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 1225
TasI AATT 6 cut(s) 804, 810, 1001, 1042, 1242, 1420
TatI WGTACW 1 cut(s) 1474
TauI GCSGC 2 cut(s) 8, 1520
TfiI GAWTC 3 cut(s) 735, 950, 1187
Tru1I TTAA 4 cut(s) 111, 1086, 1193, 1368
Tru9I TTAA 4 cut(s) 111, 1086, 1193, 1368
TscAI CASTG 4 cut(s) 334, 510, 970, 1519
TseFI GTSAC 3 cut(s) 235, 877, 1510
Tsp45I GTSAC 3 cut(s) 235, 877, 1510
TspDTI ATGAA 3 cut(s) 33, 1179, 1240
TspGWI ACGGA 1 cut(s) 1316
TspRI CASTG 4 cut(s) 334, 510, 970, 1519
VpaK11BI GGWCC 3 cut(s) 444, 1014, 1256
VspI ATTAAT 1 cut(s) 1193
XapI RAATTY 2 cut(s) 810, 1420
XcmI CCANNNNNNNNNTGG 2 cut(s) 454, 574
XspI CTAG 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.