Prupe.7G192400_v2.0.a1

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0017963
Physical Location & Seq
Forward (+)
18357081 .. 18359221
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G192400.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1665 bp
ATGAAGCGGTCAAGAAACTGCATCAAGATGAACATGCTTATGTTCTTTCCCTCCTTAACATCTTTGCTTCTAATGGCTTCCTTCTTCTTCTTCCACTTGGGCTTCTCTTTCAGCCACCAAATTCCACACCCCACAATCAAGAACGCTTGCACCACTCTTCTTCACCCATCTCTGTGCTTCACTGCCCTTGCCTCTGCCAAAACCCATGACCATTTCACTGCATTTCATCATCTTCTTGAGGCCAACATCAACCAAACTGTGGCATCTGTGGAAAGTACCAGGCAGAGCATTAAGGGTCTTCTGAAGCTCCAAGATTTGTATTTGCAAGAGAGGAATGCGCTGAAAGATTGTCTAGAGATGTTAGACCAAACCCTTTATGAACTTGGCCAGGCAGTTGATGATTTGCATGGATTTCGTGTGTCCTACGGTAACCTCAAGACTCTTCTTAGTGCAGCTATGACAAATGAGAACACTTGCATTGATGGGTTTTTTGAATTGGAAGAATTTGATTCAGAAAATCAAATCGGTCTCAATGGTCACCTCCAAGGCTTGTTGGCTCCCATCTCTGAATTGATGAGTGATTGCTTGGCCATGATCAGATACCAAGAACAAGCCCTAAATGAACAGAAGAGGCTTGTTACCAAGAAACCAAAGAAGAGGTTCCCAGTGTGGATGCTGCCCGGTGATCGAAATCTGATGCAGAGAGAACCAACAATGAAGGCTGACATTGTTGTTGCCGCTGATGGCAATGGAGATTATGAGACTATTGGTGAAGCACTGAAGATGGCTCCTAACATGAGCAGGACCAGGTTTGTGATTAAAATAAAGGCAGGAGTTTACAATGAGACCGTAGAGATTTCCAGAGAAAAGGCTAATATCATGTTGGTTGGAGATGGGATGAACACAACCATAATCACAGGGTCTAAAAGCTTTGCAGACGGATTTTCCACCTTTGCTACAGCCACTTTAACTGTAATTGGGGACAGGTTTTTAGCCAGGGACTTGACCATAACCAACACAGCAGGTTCAAAGAAGTTCCAGGCCGTTGCTGCAAGAGTGACCTCTAACTCTGCCTTCTATCATTGCAACTTCAGTTCTTATCAAGACACCCTTTATGTTCATTCCCTCCGCCAATTCTACCGTGACTGCATAATCGAAGGCACGATTGATTTCATTTTCGGAAATGCAGCTGCAGTCTTCCAAAACTGCACAGTAATTGTTCGAAAACCGATTCCCGGTCAGAAATTGATGATCACTGCTCAAGGGAGAACAGACCCAAATCAAAACACTGGCATTTCGTTGCAGAACTGTACCATTGTGGCTGCACCAGATTTCAACAAGACTGAGAGGCAAAATTTCACAACTTTTTTGGGTAGGCCTTGGAGAAACTATTCAAGGACTATAGTCATGAAGAGCTACTTGGGGGACATGATAAATCCACAAGGGTGGTCAAAGTGGGATGATTATAGCACTGTGGAAACTGTGGAGTACATAGAGTACTTGAATTTTGGACCTGGATCAGATACGAGGCACAGGGTCAAATGGGGAGGCTATAAAAAGAATTGCAGTGAAGAGATTGCGAGGCAGTTTACAGTTGGTTTGTTCTTACATGGAGCAGGTCATTGGCTAAAGACAACGGGCATTCCACTTTCGTATGGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

555

Amino Acids

62.36

Weight (kDa)

8.5

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1013, 1607
AccB7I CCANNNNNTGG 1 cut(s) 259
AciI CCGC 3 cut(s) 7, 738, 1129
AclWI GGATC 1 cut(s) 1525
AcoI YGGCCR 2 cut(s) 385, 588
AcsI RAATTY 4 cut(s) 120, 503, 1354, 1504
AcuI CTGAAG 3 cut(s) 323, 800, 1075
AfaI GTAC 4 cut(s) 277, 1312, 1490, 1499
AfiI CCNNNNNNNGG 2 cut(s) 259, 1235
AgsI TTSAA 5 cut(s) 494, 1029, 1336, 1395, 1504
AjnI CCWGG 6 cut(s) 278, 387, 806, 995, 1038, 1513
AjuI GAANNNNNNNTTGG 2 cut(s) 1403, 1435
AleI CACNNNNGTG 1 cut(s) 1444
AluBI AGCT 5 cut(s) 307, 455, 930, 1190, 1416
AluI AGCT 5 cut(s) 307, 455, 930, 1190, 1416
Alw26I GTCTC 3 cut(s) 533, 755, 839
AlwI GGATC 1 cut(s) 1525
AoxI GGCC 5 cut(s) 240, 385, 588, 1041, 1376
ApeKI GCWGC 6 cut(s) 452, 676, 1049, 1187, 1190, 1322
ApoI RAATTY 4 cut(s) 120, 503, 1354, 1504
Asp700I GAANNNNTTC 2 cut(s) 659, 1390
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 804, 1511
AsuC2I CCSGG 2 cut(s) 681, 1236
AsuHPI GGTGA 4 cut(s) 155, 530, 695, 782
AsuII TTCGAA 1 cut(s) 1222
AvaII GGWCC 2 cut(s) 804, 1511
BalI TGGCCA 2 cut(s) 387, 590
BbsI GAAGAC 2 cut(s) 290, 1189
BbvI GCAGC 6 cut(s) 464, 663, 1036, 1177, 1199, 1309
BccI CCATC 6 cut(s) 175, 476, 569, 737, 778, 887
BceAI ACGGC 1 cut(s) 1028
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 6 cut(s) 280, 389, 808, 997, 1040, 1515
BclI TGATCA 2 cut(s) 594, 1251
BcnI CCSGG 2 cut(s) 681, 1236
BcoDI GTCTC 3 cut(s) 533, 755, 839
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 3 cut(s) 957, 1191, 1401
BfuAI ACCTGC 2 cut(s) 1013, 1607
BisI GCNGC 7 cut(s) 453, 677, 738, 1050, 1188, 1191, 1323
BlsI GCNGC 7 cut(s) 454, 678, 739, 1051, 1189, 1192, 1324
BmcAI AGTACT 1 cut(s) 1499
Bme1390I CCNGG 8 cut(s) 280, 389, 681, 808, 997, 1040, 1236, 1515
Bme18I GGWCC 2 cut(s) 804, 1511
BmgT120I GGNCC 2 cut(s) 804, 1511
BmiI GGNNCC 3 cut(s) 558, 662, 789
BmrFI CCNGG 8 cut(s) 280, 389, 681, 808, 997, 1040, 1236, 1515
BmrI ACTGGG 1 cut(s) 659
BmsI GCATC 4 cut(s) 30, 272, 663, 687
BmuI ACTGGG 1 cut(s) 659
BoxI GACNNNNGTC 1 cut(s) 1403
BpiI GAAGAC 2 cut(s) 290, 1189
Bpu14I TTCGAA 1 cut(s) 1222
BpuEI CTTGAG 3 cut(s) 257, 419, 1245
BpuMI CCSGG 2 cut(s) 681, 1236
BsaBI GATNNNNATC 1 cut(s) 690
BsaI GGTCTC 2 cut(s) 533, 839
BsaJI CCNNGG 3 cut(s) 544, 996, 1379
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 1235
Bse1I ACTGG 2 cut(s) 665, 1294
Bse3DI GCAATG 2 cut(s) 754, 1081
Bse8I GATNNNNATC 1 cut(s) 690
BseBI CCWGG 6 cut(s) 280, 389, 808, 997, 1040, 1515
BseDI CCNNGG 3 cut(s) 544, 996, 1379
BseGI GGATG 3 cut(s) 678, 903, 1465
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 2 cut(s) 259, 1235
BseMI GCAATG 2 cut(s) 754, 1081
BseMII CTCAG 1 cut(s) 1335
BseNI ACTGG 2 cut(s) 665, 1294
BseXI GCAGC 6 cut(s) 464, 663, 1036, 1177, 1199, 1309
BsgI GTGCAG 3 cut(s) 471, 1192, 1308
BshFI GGCC 5 cut(s) 242, 387, 590, 1043, 1378
BsiSI CCGG 2 cut(s) 681, 1236
BslFI GGGAC 3 cut(s) 995, 1013, 1439
BslI CCNNNNNNNGG 2 cut(s) 259, 1235
BsmAI GTCTC 3 cut(s) 533, 755, 839
BsmFI GGGAC 3 cut(s) 995, 1013, 1439
BsmI GAATGC 2 cut(s) 340, 1641
BsnI GGCC 5 cut(s) 242, 387, 590, 1043, 1378
Bso31I GGTCTC 2 cut(s) 533, 839
Bsp119I TTCGAA 1 cut(s) 1222
Bsp143I GATC 4 cut(s) 594, 685, 1251, 1517
BspACI CCGC 3 cut(s) 7, 738, 1129
BspANI GGCC 5 cut(s) 242, 387, 590, 1043, 1378
BspCNI CTCAG 1 cut(s) 1336
BspHI TCATGA 1 cut(s) 1407
BspLI GGNNCC 3 cut(s) 558, 662, 789
BspMAI CTGCAG 1 cut(s) 1195
BspMI ACCTGC 2 cut(s) 1013, 1607
BspPI GGATC 1 cut(s) 1525
BspQI GCTCTTC 1 cut(s) 1406
BspT104I TTCGAA 1 cut(s) 1222
BspTNI GGTCTC 2 cut(s) 533, 839
BsrDI GCAATG 2 cut(s) 754, 1081
BsrI ACTGG 2 cut(s) 665, 1294
BssECI CCNNGG 3 cut(s) 544, 996, 1379
BssMI GATC 4 cut(s) 594, 685, 1251, 1517
BssT1I CCWWGG 2 cut(s) 544, 1379
Bst2UI CCWGG 6 cut(s) 280, 389, 808, 997, 1040, 1515
Bst6I CTCTTC 6 cut(s) 162, 447, 623, 650, 1406, 1566
BstBI TTCGAA 1 cut(s) 1222
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 2 cut(s) 446, 1344
BstEII GGTNACC 2 cut(s) 428, 536
BstF5I GGATG 3 cut(s) 678, 903, 1465
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 4 cut(s) 597, 688, 1254, 1520
BstMAI GTCTC 3 cut(s) 533, 755, 839
BstMBI GATC 4 cut(s) 594, 685, 1251, 1517
BstMWI GCNNNNNNNGC 1 cut(s) 1049
BstNI CCWGG 6 cut(s) 280, 389, 808, 997, 1040, 1515
BstNSI RCATGY 1 cut(s) 37
BstPAI GACNNNNGTC 1 cut(s) 1403
BstPI GGTNACC 2 cut(s) 428, 536
BstSCI CCNGG 8 cut(s) 278, 387, 679, 806, 995, 1038, 1234, 1513
BstSFI CTRYAG 3 cut(s) 957, 1191, 1401
BstV1I GCAGC 6 cut(s) 464, 663, 1036, 1177, 1199, 1309
BstV2I GAAGAC 2 cut(s) 290, 1189
BstXI CCANNNNNNTGG 1 cut(s) 1446
BsuRI GGCC 5 cut(s) 242, 387, 590, 1043, 1378
BtsCI GGATG 3 cut(s) 678, 903, 1465
BtsI GCAGTG 4 cut(s) 180, 216, 1254, 1573
BtsIMutI CAGTG 8 cut(s) 180, 216, 672, 776, 1254, 1287, 1470, 1573
BveI ACCTGC 2 cut(s) 1013, 1607
Cac8I GCNNGC 1 cut(s) 148
CciI TCATGA 1 cut(s) 1407
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 804, 1511
CsiI ACCWGGT 1 cut(s) 806
Csp6I GTAC 4 cut(s) 276, 1311, 1489, 1498
CviAII CATG 9 cut(s) 34, 206, 407, 592, 796, 880, 1408, 1429, 1610
CviQI GTAC 4 cut(s) 276, 1311, 1489, 1498
DdeI CTNAG 2 cut(s) 446, 1344
DpnI GATC 4 cut(s) 596, 687, 1253, 1519
DpnII GATC 4 cut(s) 594, 685, 1251, 1517
EaeI YGGCCR 2 cut(s) 385, 588
Eam1104I CTCTTC 6 cut(s) 162, 447, 623, 650, 1406, 1566
EarI CTCTTC 6 cut(s) 162, 447, 623, 650, 1406, 1566
EciI GGCGGA 1 cut(s) 1118
Eco130I CCWWGG 2 cut(s) 544, 1379
Eco147I AGGCCT 1 cut(s) 1378
Eco31I GGTCTC 2 cut(s) 533, 839
Eco47I GGWCC 2 cut(s) 804, 1511
Eco57I CTGAAG 3 cut(s) 323, 800, 1075
Eco91I GGTNACC 2 cut(s) 428, 536
EcoO65I GGTNACC 2 cut(s) 428, 536
EcoRII CCWGG 6 cut(s) 278, 387, 806, 995, 1038, 1513
EcoT14I CCWWGG 2 cut(s) 544, 1379
ErhI CCWWGG 2 cut(s) 544, 1379
FaeI CATG 9 cut(s) 37, 209, 410, 595, 799, 883, 1411, 1432, 1613
FalI AAGNNNNNCTT 4 cut(s) 1095, 1127, 1403, 1435
FaqI GGGAC 3 cut(s) 995, 1013, 1439
FatI CATG 9 cut(s) 33, 205, 406, 591, 795, 879, 1407, 1428, 1609
FbaI TGATCA 2 cut(s) 594, 1251
Fnu4HI GCNGC 7 cut(s) 453, 677, 738, 1050, 1188, 1191, 1323
FokI GGATG 3 cut(s) 685, 910, 1472
Fsp4HI GCNGC 7 cut(s) 453, 677, 738, 1050, 1188, 1191, 1323
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 339
GluI GCNGC 7 cut(s) 453, 677, 738, 1050, 1188, 1191, 1323
HaeIII GGCC 5 cut(s) 242, 387, 590, 1043, 1378
HapII CCGG 2 cut(s) 681, 1236
HhaI GCGC 1 cut(s) 340
Hin1II CATG 9 cut(s) 37, 209, 410, 595, 799, 883, 1411, 1432, 1613
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HindIII AAGCTT 1 cut(s) 928
HinfI GANTC 3 cut(s) 439, 509, 1231
HpaII CCGG 2 cut(s) 681, 1236
HphI GGTGA 4 cut(s) 155, 530, 695, 782
Hpy166II GTNNAC 2 cut(s) 838, 1590
Hpy188I TCNGA 8 cut(s) 303, 514, 568, 599, 696, 1181, 1242, 1522
Hpy188III TCNNGA 9 cut(s) 12, 25, 139, 236, 353, 436, 861, 1103, 1408
Hpy8I GTNNAC 2 cut(s) 838, 1590
HpyAV CCTTC 4 cut(s) 91, 712, 1084, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 1049
HpyF3I CTNAG 2 cut(s) 446, 1344
Hsp92II CATG 9 cut(s) 37, 209, 410, 595, 799, 883, 1411, 1432, 1613
HspAI GCGC 1 cut(s) 338
Ksp22I TGATCA 2 cut(s) 594, 1251
Kzo9I GATC 4 cut(s) 594, 685, 1251, 1517
LguI GCTCTTC 1 cut(s) 1406
LmnI GCTCC 4 cut(s) 312, 562, 793, 1613
Lsp1109I GCAGC 6 cut(s) 464, 663, 1036, 1177, 1199, 1309
LweI GCATC 4 cut(s) 30, 272, 663, 687
MabI ACCWGGT 1 cut(s) 806
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 5 cut(s) 428, 536, 637, 1057, 1142
MalI GATC 4 cut(s) 596, 687, 1253, 1519
MboI GATC 4 cut(s) 594, 685, 1251, 1517
MlsI TGGCCA 2 cut(s) 387, 590
MluNI TGGCCA 2 cut(s) 387, 590
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 868
Mox20I TGGCCA 2 cut(s) 387, 590
MroXI GAANNNNTTC 2 cut(s) 659, 1390
MscI TGGCCA 2 cut(s) 387, 590
MseI TTAA 4 cut(s) 56, 291, 819, 968
MslI CAYNNNNRTG 4 cut(s) 26, 38, 172, 1444
Msp20I TGGCCA 2 cut(s) 387, 590
MspA1I CMGCKG 2 cut(s) 740, 1190
MspI CCGG 2 cut(s) 681, 1236
MspR9I CCNGG 8 cut(s) 280, 389, 681, 808, 997, 1040, 1236, 1515
Mva1269I GAATGC 2 cut(s) 340, 1641
MvaI CCWGG 6 cut(s) 280, 389, 808, 997, 1040, 1515
MwoI GCNNNNNNNGC 1 cut(s) 1049
NciI CCSGG 2 cut(s) 681, 1236
NdeII GATC 4 cut(s) 594, 685, 1251, 1517
NlaIII CATG 9 cut(s) 37, 209, 410, 595, 799, 883, 1411, 1432, 1613
NlaIV GGNNCC 3 cut(s) 558, 662, 789
NmuCI GTSAC 3 cut(s) 536, 1057, 1142
NspI RCATGY 1 cut(s) 37
NspV TTCGAA 1 cut(s) 1222
OliI CACNNNNGTG 1 cut(s) 1444
PagI TCATGA 1 cut(s) 1407
PceI AGGCCT 1 cut(s) 1378
PciSI GCTCTTC 1 cut(s) 1406
PctI GAATGC 2 cut(s) 340, 1641
PdmI GAANNNNTTC 2 cut(s) 659, 1390
PfeI GAWTC 2 cut(s) 509, 1231
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 7 cut(s) 454, 678, 739, 1051, 1189, 1192, 1324
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PshAI GACNNNNGTC 1 cut(s) 1403
Psp6I CCWGG 6 cut(s) 278, 387, 806, 995, 1038, 1513
PspEI GGTNACC 2 cut(s) 428, 536
PspGI CCWGG 6 cut(s) 278, 387, 806, 995, 1038, 1513
PspN4I GGNNCC 3 cut(s) 558, 662, 789
PspPI GGNCC 2 cut(s) 804, 1511
PstI CTGCAG 1 cut(s) 1195
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 4 cut(s) 277, 1312, 1490, 1499
RsaNI GTAC 4 cut(s) 276, 1311, 1489, 1498
RseI CAYNNNNRTG 4 cut(s) 26, 38, 172, 1444
SapI GCTCTTC 1 cut(s) 1406
SaqAI TTAA 4 cut(s) 56, 291, 819, 968
SatI GCNGC 7 cut(s) 453, 677, 738, 1050, 1188, 1191, 1323
Sau3AI GATC 4 cut(s) 594, 685, 1251, 1517
Sau96I GGNCC 2 cut(s) 804, 1511
ScaI AGTACT 1 cut(s) 1499
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 8 cut(s) 280, 389, 681, 808, 997, 1040, 1236, 1515
SexAI ACCWGGT 1 cut(s) 806
SfaNI GCATC 4 cut(s) 30, 272, 663, 687
SfcI CTRYAG 3 cut(s) 957, 1191, 1401
SfuI TTCGAA 1 cut(s) 1222
SinI GGWCC 2 cut(s) 804, 1511
SmiMI CAYNNNNRTG 4 cut(s) 26, 38, 172, 1444
SmlI CTYRAG 3 cut(s) 236, 434, 1260
SmoI CTYRAG 3 cut(s) 236, 434, 1260
SseBI AGGCCT 1 cut(s) 1378
SsiI CCGC 3 cut(s) 7, 738, 1129
SspMI CTAG 1 cut(s) 353
StuI AGGCCT 1 cut(s) 1378
StyD4I CCNGG 8 cut(s) 278, 387, 679, 806, 995, 1038, 1234, 1513
StyI CCWWGG 2 cut(s) 544, 1379
TaqI TCGA 3 cut(s) 688, 1155, 1222
TaqII GACCGA 1 cut(s) 515
TatI WGTACW 2 cut(s) 1488, 1497
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 2 cut(s) 509, 1231
Tru1I TTAA 4 cut(s) 56, 291, 819, 968
Tru9I TTAA 4 cut(s) 56, 291, 819, 968
TscAI CASTG 8 cut(s) 187, 223, 672, 783, 1261, 1294, 1477, 1573
TseFI GTSAC 3 cut(s) 536, 1057, 1142
TseI GCWGC 6 cut(s) 452, 676, 1049, 1187, 1190, 1322
Tsp45I GTSAC 3 cut(s) 536, 1057, 1142
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 8 cut(s) 187, 223, 672, 783, 1261, 1294, 1477, 1573
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 804, 1511
XapI RAATTY 4 cut(s) 120, 503, 1354, 1504
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 1 cut(s) 37
XmnI GAANNNNTTC 2 cut(s) 659, 1390
XspI CTAG 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.