pycom01g03990

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014228
Physical Location & Seq
Forward (+)
3049331 .. 3050440
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03990.1

Sequence Viewer

Length: 1110 bp
ATGCAACTCCTCCACACTAAAGACTTGCTCCCCAACCGCAACTTGCAGCGCCTCATCCAGATCTGGTCCGACTCCCTCCGCCTCCCCCCACGCAACTCCACCTCCTCCTCGCCACCCTCTACACCCTTCCAACCACCACTGCCCAAGGACCAGATCCGCCACCTAATCCACCGCTTGGACGCCTATACCGAACCATCTTCCTCCTTCGATTCCCTTTCCAAGATTGCCCAGTTCGCAAGAGAGTCCGACGACAACAGCAAAATCCTCGCCGAAAGCGATGGTTTCATCCAAGTCGTCGTCGAGTTTCTCGGTGGCAGCGGCAATGCCGAAGACAAAAACATCGGCGCCCTCGAACAAGTTGTGAGAATCCTTGATCTGGTGAAGGACAAAATACAAGACCGGGAGGAGCTGTCGAGATGGATGCTAAAAAAGAGCGGTCAGGATTGCGTGGCTTCTCTGCTTCTTATTGCACAACATGGAAGCGTGGATTCTCGAATCGCATCGGCTGGGGTTTTGGAATCAATCGCGGTAAACGCGGAAGCAAAAATCCTGATAGCCGAGAAAGAAGGGCTTTTACCCCAGCTGCTTAAGTCGACGGCTCCGGAAAATGATCCGACGGTCATTGAAGCGAGTCTCTCTTGCTTGATCGCCCTGTCGACCCCGAAGCGAGTGAAGGTTAGATTGGTCCATTTAGGAGCAGTGAAGCTGCTGTCCAAGTTACTGTGGGACCCGAATTCCACTACGGCGACGGTGGAGGAGGCGTTGAAGTTGCTGGAAACGGTGTCGTCGGTGAAAGAGGGGCGGGGAAAGATATGCGAGGACGGCAAGTGTGTGTCGGCGATCGTGCAGAGGCTGCTGAAGGTGTCGAGTGCGGCGACGGAGCACGCGGTGACGATACTATGGAGCGTGTGCTATTTGTTTAAGGAAAGGGCGGCGCAAGAGGCGGTGGGGAGGGCGAATGGGTTGACCAAGATATTGCTGCTGATGCAAAGCAATTGCTCGCCGGCTGTTCGGCAAATGTGTGCCGATTTGCTCAAGATTTTTCGTGTGAATTCGAAATCGGTTATTTCTTGTTATGATACTAAGACCACTCATATCATGCCCTTCTGA

Protein Analysis

370

Amino Acids

40.51

Weight (kDa)

8.54

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 51 - 366 6.9e-78 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09800 AT5G64660
fragaria_vesca FvH4_1g24000
malus_domestica MD01G1010900.v1.1
prunus_persica Prupe.6G159600_v2.0.a1
pyrus_communis pycom01g03990 pycom1353g00040
rosa_chinensis RchiOBHm_Chr2g0120131
rosa_laevigata RLG00000018524
rosa_multiflora Rmu_sc0016643.1_g000003
rosa_roxburghii Rroxscaffold_2G00123700
rosa_rugosa Rorug02G0227000
rosa_samantha Rh2AG283600 Rh2BG294900 Rh2CG272300 Rh2DG308600
rosa_wichuraiana Rw2G022710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 344
AccB7I CCANNNNNTGG 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 435
AccI GTMKAC 2 cut(s) 593, 656
AccII CGCG 3 cut(s) 527, 536, 887
AccIII TCCGGA 1 cut(s) 601
AclWI GGATC 2 cut(s) 148, 605
AcsI RAATTY 2 cut(s) 733, 1051
AcuI CTGAAG 1 cut(s) 878
AcyI GRCGYC 2 cut(s) 180, 345
AdeI CACNNNGTG 1 cut(s) 889
AfiI CCNNNNNNNGG 2 cut(s) 175, 376
AflII CTTAAG 1 cut(s) 587
AgsI TTSAA 2 cut(s) 626, 766
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 3 cut(s) 409, 583, 706
AluI AGCT 3 cut(s) 409, 583, 706
Alw21I GWGCWC 1 cut(s) 885
Alw26I GTCTC 1 cut(s) 638
AlwI GGATC 2 cut(s) 148, 605
AlwNI CAGNNNCTG 1 cut(s) 853
Aor13HI TCCGGA 1 cut(s) 601
ApeKI GCWGC 6 cut(s) 46, 315, 583, 706, 853, 979
ApoI RAATTY 2 cut(s) 733, 1051
AspLEI GCGC 3 cut(s) 51, 347, 937
AspS9I GGNCC 4 cut(s) 66, 148, 685, 727
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 391, 802, 901
AsuII TTCGAA 1 cut(s) 1055
AvaII GGWCC 4 cut(s) 66, 148, 685, 727
BanI GGYRCC 1 cut(s) 344
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 885
BbvI GCAGC 6 cut(s) 58, 327, 570, 693, 840, 966
BccI CCATC 3 cut(s) 202, 272, 411
BceAI ACGGC 3 cut(s) 612, 759, 838
BcgI CGANNNNNNTGC 4 cut(s) 247, 281, 403, 437
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 1 cut(s) 638
BfoI RGCGCY 2 cut(s) 52, 348
BfrI CTTAAG 1 cut(s) 587
BglII AGATCT 1 cut(s) 60
BisI GCNGC 9 cut(s) 47, 316, 319, 584, 707, 854, 873, 933, 980
BlsI GCNGC 9 cut(s) 48, 317, 320, 585, 708, 855, 874, 934, 981
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 4 cut(s) 66, 148, 685, 727
BmgT120I GGNCC 4 cut(s) 66, 148, 685, 727
BmiI GGNNCC 4 cut(s) 346, 600, 728, 729
BmrFI CCNGG 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 3 cut(s) 411, 509, 975
BmuI ACTGGG 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 1055
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 1 cut(s) 401
BsaHI GRCGYC 2 cut(s) 180, 345
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 6 cut(s) 86, 116, 395, 425, 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 376
Bse118I RCCGGY 1 cut(s) 1003
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 328
BseAI TCCGGA 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 3 cut(s) 54, 285, 426
BseLI CCNNNNNNNGG 2 cut(s) 175, 376
BseMI GCAATG 1 cut(s) 328
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 4 cut(s) 94, 97, 419, 770
BseXI GCAGC 6 cut(s) 58, 327, 570, 693, 840, 966
BseYI CCCAGC 2 cut(s) 506, 579
BsgI GTGCAG 1 cut(s) 866
Bsh1236I CGCG 3 cut(s) 527, 536, 887
Bsh1285I CGRYCG 1 cut(s) 843
BshNI GGYRCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 885
BsiSI CCGG 3 cut(s) 400, 602, 1004
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 2 cut(s) 175, 376
BsmAI GTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 740
Bsp119I TTCGAA 1 cut(s) 1055
Bsp1286I GDGCHC 1 cut(s) 885
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 6 cut(s) 60, 153, 373, 610, 645, 840
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 3 cut(s) 527, 536, 887
BspLI GGNNCC 4 cut(s) 346, 600, 728, 729
BspPI GGATC 2 cut(s) 148, 605
BspT104I TTCGAA 1 cut(s) 1055
BspT107I GGYRCC 1 cut(s) 344
BspTI CTTAAG 1 cut(s) 587
BsrBI CCGCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 1003
BsrI ACTGG 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 6 cut(s) 60, 153, 373, 610, 645, 840
BssNI GRCGYC 2 cut(s) 180, 345
BssT1I CCWWGG 1 cut(s) 144
Bst4CI ACNGT 4 cut(s) 619, 723, 751, 781
BstACI GRCGYC 2 cut(s) 180, 345
BstAFI CTTAAG 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 1055
BstC8I GCNNGC 3 cut(s) 885, 1001, 1005
BstDEI CTNAG 1 cut(s) 1083
BstF5I GGATG 3 cut(s) 54, 285, 426
BstFNI CGCG 3 cut(s) 527, 536, 887
BstH2I RGCGCY 2 cut(s) 52, 348
BstHHI GCGC 3 cut(s) 51, 347, 937
BstKTI GATC 6 cut(s) 63, 156, 376, 613, 648, 843
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 6 cut(s) 60, 153, 373, 610, 645, 840
BstMCI CGRYCG 1 cut(s) 843
BstMWI GCNNNNNNNGC 6 cut(s) 233, 533, 822, 853, 941, 985
BstSCI CCNGG 1 cut(s) 399
BstUI CGCG 3 cut(s) 527, 536, 887
BstV1I GCAGC 6 cut(s) 58, 327, 570, 693, 840, 966
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 60, 153
BstYI RGATCY 2 cut(s) 60, 153
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 54, 285, 426
BtsI GCAGTG 2 cut(s) 137, 705
BtsIMutI CAGTG 2 cut(s) 137, 705
Cac8I GCNNGC 3 cut(s) 885, 1001, 1005
CaiI CAGNNNCTG 1 cut(s) 853
CfoI GCGC 3 cut(s) 51, 347, 937
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 4 cut(s) 66, 148, 685, 727
CseI GACGC 1 cut(s) 188
CviAII CATG 2 cut(s) 476, 1099
DdeI CTNAG 1 cut(s) 1083
DinI GGCGCC 1 cut(s) 346
DpnI GATC 6 cut(s) 62, 155, 375, 612, 647, 842
DpnII GATC 6 cut(s) 60, 153, 373, 610, 645, 840
DraIII CACNNNGTG 1 cut(s) 889
EciI GGCGGA 2 cut(s) 68, 146
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 4 cut(s) 66, 148, 685, 727
Eco57I CTGAAG 1 cut(s) 878
EcoO109I RGGNCCY 1 cut(s) 727
EcoRI GAATTC 2 cut(s) 733, 1051
EcoT14I CCWWGG 1 cut(s) 144
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 2 cut(s) 479, 1102
FaiI YATR 7 cut(s) 186, 477, 814, 901, 1077, 1095, 1100
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FaqI GGGAC 1 cut(s) 740
FatI CATG 2 cut(s) 475, 1098
FauI CCCGC 1 cut(s) 795
FblI GTMKAC 2 cut(s) 593, 656
Fnu4HI GCNGC 9 cut(s) 47, 316, 319, 584, 707, 854, 873, 933, 980
FokI GGATG 3 cut(s) 41, 272, 433
Fsp4HI GCNGC 9 cut(s) 47, 316, 319, 584, 707, 854, 873, 933, 980
GlaI GCGC 3 cut(s) 50, 346, 936
GluI GCNGC 9 cut(s) 47, 316, 319, 584, 707, 854, 873, 933, 980
GsaI CCCAGC 2 cut(s) 510, 583
HaeII RGCGCY 2 cut(s) 52, 348
HapII CCGG 3 cut(s) 400, 602, 1004
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 51, 347, 937
Hin1I GRCGYC 2 cut(s) 180, 345
Hin1II CATG 2 cut(s) 479, 1102
Hin6I GCGC 3 cut(s) 49, 345, 935
HinP1I GCGC 3 cut(s) 49, 345, 935
HincII GTYRAC 3 cut(s) 594, 657, 966
HindII GTYRAC 3 cut(s) 594, 657, 966
HinfI GANTC 8 cut(s) 71, 209, 242, 366, 488, 495, 518, 631
HpaII CCGG 3 cut(s) 400, 602, 1004
HphI GGTGA 3 cut(s) 391, 802, 901
Hpy166II GTNNAC 4 cut(s) 532, 594, 657, 966
Hpy188I TCNGA 4 cut(s) 70, 247, 615, 1109
Hpy188III TCNNGA 7 cut(s) 58, 414, 440, 492, 550, 602, 1036
Hpy8I GTNNAC 4 cut(s) 532, 594, 657, 966
Hpy99I CGWCG 8 cut(s) 251, 299, 302, 598, 619, 751, 790, 880
HpyAV CCTTC 6 cut(s) 136, 214, 376, 560, 667, 853
HpyCH4III ACNGT 4 cut(s) 619, 723, 751, 781
HpyCH4V TGCA 5 cut(s) 4, 46, 470, 847, 988
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 533, 822, 853, 941, 985
HpyF3I CTNAG 1 cut(s) 1083
Hsp92I GRCGYC 2 cut(s) 180, 345
Hsp92II CATG 2 cut(s) 479, 1102
HspAI GCGC 3 cut(s) 49, 345, 935
KasI GGCGCC 1 cut(s) 344
KflI GGGWCCC 1 cut(s) 727
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 1003
KroNI GCCGGC 1 cut(s) 1005
Kzo9I GATC 6 cut(s) 60, 153, 373, 610, 645, 840
LmnI GCTCC 6 cut(s) 33, 406, 604, 695, 880, 903
Lsp1109I GCAGC 6 cut(s) 58, 327, 570, 693, 840, 966
LweI GCATC 3 cut(s) 411, 509, 975
MaeIII GTNAC 2 cut(s) 717, 889
MalI GATC 6 cut(s) 62, 155, 375, 612, 647, 842
MbiI CCGCTC 1 cut(s) 435
MboI GATC 6 cut(s) 60, 153, 373, 610, 645, 840
MboII GAAGA 2 cut(s) 189, 341
MfeI CAATTG 1 cut(s) 994
MflI RGATCY 2 cut(s) 60, 153
MhlI GDGCHC 1 cut(s) 885
MluCI AATT 3 cut(s) 733, 994, 1051
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 3 cut(s) 65, 251, 640
MmeI TCCRAC 4 cut(s) 93, 154, 270, 638
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 1003
MseI TTAA 2 cut(s) 588, 921
MspA1I CMGCKG 2 cut(s) 318, 583
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 3 cut(s) 400, 602, 1004
MspR9I CCNGG 1 cut(s) 401
MunI CAATTG 1 cut(s) 994
MvnI CGCG 3 cut(s) 527, 536, 887
MwoI GCNNNNNNNGC 6 cut(s) 233, 533, 822, 853, 941, 985
NaeI GCCGGC 1 cut(s) 1005
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 1 cut(s) 401
NdeII GATC 6 cut(s) 60, 153, 373, 610, 645, 840
NgoMIV GCCGGC 1 cut(s) 1003
NlaIII CATG 2 cut(s) 479, 1102
NlaIV GGNNCC 4 cut(s) 346, 600, 728, 729
NmeAIII GCCGAG 1 cut(s) 583
NmuCI GTSAC 1 cut(s) 889
NspV TTCGAA 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 273, 348, 872
PdiI GCCGGC 1 cut(s) 1005
PfeI GAWTC 5 cut(s) 209, 366, 488, 495, 518
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 9 cut(s) 48, 317, 320, 585, 708, 855, 874, 934, 981
Ple19I CGATCG 1 cut(s) 843
PleI GAGTC 3 cut(s) 65, 250, 639
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 3 cut(s) 65, 250, 639
PpuMI RGGWCCY 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 727
PspFI CCCAGC 2 cut(s) 506, 579
PspN4I GGNNCC 4 cut(s) 346, 600, 728, 729
PspPI GGNCC 4 cut(s) 66, 148, 685, 727
PspPPI RGGWCCY 1 cut(s) 727
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 2 cut(s) 60, 153
PvuI CGATCG 1 cut(s) 843
PvuII CAGCTG 1 cut(s) 583
SalI GTCGAC 2 cut(s) 592, 655
SaqAI TTAA 2 cut(s) 588, 921
SatI GCNGC 9 cut(s) 47, 316, 319, 584, 707, 854, 873, 933, 980
Sau3AI GATC 6 cut(s) 60, 153, 373, 610, 645, 840
Sau96I GGNCC 4 cut(s) 66, 148, 685, 727
SchI GAGTC 3 cut(s) 65, 251, 640
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 885
SetI ASST 7 cut(s) 104, 165, 411, 585, 678, 708, 864
SfaNI GCATC 3 cut(s) 411, 509, 975
SfoI GGCGCC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 1055
SinI GGWCC 4 cut(s) 66, 148, 685, 727
SmlI CTYRAG 2 cut(s) 587, 1034
SmoI CTYRAG 2 cut(s) 587, 1034
Sse9I AATT 3 cut(s) 733, 994, 1051
SspDI GGCGCC 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 619, 723, 751, 781
TaqI TCGA 9 cut(s) 207, 300, 351, 413, 493, 593, 656, 866, 1055
TasI AATT 3 cut(s) 733, 994, 1051
TauI GCSGC 3 cut(s) 321, 875, 935
TfiI GAWTC 5 cut(s) 209, 366, 488, 495, 518
Tru1I TTAA 2 cut(s) 588, 921
Tru9I TTAA 2 cut(s) 588, 921
TscAI CASTG 2 cut(s) 144, 705
TseFI GTSAC 1 cut(s) 889
TseI GCWGC 6 cut(s) 46, 315, 583, 706, 853, 979
Tsp45I GTSAC 1 cut(s) 889
TspDTI ATGAA 1 cut(s) 274
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 2 cut(s) 144, 705
Van91I CCANNNNNTGG 1 cut(s) 175
Vha464I CTTAAG 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 66, 148, 685, 727
XapI RAATTY 2 cut(s) 733, 1051
XmiI GTMKAC 2 cut(s) 593, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.