pycom01g04600
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012455
Physical Location & Seq
Reverse (-)
3572117 .. 3575419
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3252 bp
ATGGCCCTTGACTCCCTTGAATCTCTCTCTCTCAAATCTGTTTCTCTCTCCGGCTCCATCTCCCTCCACTTCCCTCCCGGATCCCAGTGCAGCCCTCTCCTCACCTCCTTAGATCTGGCTCACAACTCCCTCTCTGGCCCTCTTTCCGACGTCCCCAACTTCGCCGCCGCTTGCTCCGCCTTGACCTTCCTCAACCTCTCCTCAAACTCTCTCGATTTCTCCACCATACCCTCCTCATCTGCCTTCCCCCTCCGCACCGTCCAGGTTCTCGATCTTTCTTACAACAAGATTACAGGCCCCAACGTCGTCCCTTGGATCTTATCCGATGGTTGCGGTAACTTGCAGAGGTTGGTTTTAAAAGGGAACAAAATCTCCGGCGAGATGAGCGTTGTCTCCACCTGCAGCAAGCTGGAGCACTTGGACTTGTCCTCCAACAACTTCTCCATTTCTCTTCCTTCTTTCGGCGATTGCTCGGCTTTGGACCACCTTGACATCTCTGGCAACAAGTTTTCTGGCGACGTTGGCCGCGCTATCTCCTCCTGCAAGCAGCTCACTTTCTTGAACCTCTCTATGAACCACTTCAACGGTCCGATTCCAGTCATGCCCACCAACAGTTTGAAGTTCCTGTCCCTTGGAGGGAATGGGTTTCAAGGTATAATTCCTATGAGCTTAATGGATTCGTGTGCAGAGCTTGTGGAGCTCGATCTGTCGGCAAATAGCCTTTCGGGGTCGGTTCCTGATGCATTGAGCTCTTGTTCTTTGTTGGAGTCGCTGGACATATCCGGCAATAACTTTTCCAGTGAGTTGCCCGTTGAGATTTTGATGAAGCTCGCCAACTTAAAGGCTGTCTCGCTTTCTTTCAACAATTTCTATGGTACTCTGCCCGATTCTCTGTCTAAGGTCGCCACATTGGAGAGTTTGGATCTCAGCTCCAACAACTTTTCTGGGTCAATCCCAGCTGGGCTGTGCGGGGATCCTGGCAACAGCTGGAAGGAGCTGTACCTTCAGAACAATCTGTTTACCGGCACGATTCCTCCGTCTCTGAGTAACTGTTCTCAGCTTGTTTCTCTTGATTTGAGCTTCAATTACCTCAAGGGCAACATCCCTTCGAGCTTGGGGTCATTGTCCAAGCTTCGCGATTTGATCATTTGGTTGAACCAGCTGAGTGGGGAAATCCCACAGGAGCTGATGTACCTTGGGTCGCTTGAGAATCTCATTCTGGACTTCAACGAGCTTACAGGGTCGATTCCCATCGGTCTAAGCAACTGCACCAATTTGAATTGGATTTCATTGGCAAACAACAAGTTGAGTGGTGAGGTTCCCGGGTGGATTGGGAAGCTTCCAAATCTAGCAATACTCAAGCTCAGTAACAACTCATTCTCTGGTAGTATTCCCCCAGAGCTCGGTGACTGTAAGAGCTTGATATGGTTGGATCTCAATACCAACATGTTGAATGGCACAATCCCTCCTGCGCTTTTCAAGCAATCCGGAAACATTGCAGTGAATTTTGTTGCTTCCAAAACGTATGTTTATATCAAGAATGATGGTAGCAAGGAGTGCCATGGAGCAGGAAATTTGCTCGAGTTTGCGGGGATCAGAACTCAGCAGCTGAACAGGATTTCGACAAGAAATCCCTGCAACTTCACAAGAGTATACAGAGGTATTCTCCAACCAACGTTTAACCATAACGGTTCTATGATTTTCCTTGACCTTTCGCATAACTCCTTGTCCGGTAGCATTCCCAAAGAGATTGGGAGCATGTACTATCTCTATATTTTGAATTTGGGCGATAACAATATATCTGGGTCAATCCCTCAAGAGCTTGGAAAAATGACCAGCCTTAACATTCTTGATCTCTCTAGCAATAGCCTCGCGGGGACTATTCCACCGGCTCTGAGTGGCCTTACCTTGCTAACTGAGATCGATCTTTCAAACAACCTTTTGTCTGGAATGATTCCAGAGTCGGGTCAGTTTGAGACGTTCCCTGCCAACAGATTCGCCAACAATTCTGGCCTCTGTGGCTATCCATTGACTTCGTGTGGGGGGGCTTTGGGGCCAATTGCAAATACACATCAAAAGTCCCATCGACGACAAGCATCCCTGGCAGGCAGTGTGGCAACGGGGTTACTCATCGCACTTTTCTGCATCTTTGGTTTGCTTATTGTTGCCATTGAAACCAAGAAAAGGCGAAAAAAGAAGGAAACAGCCCTTGATGTTTATATTGACAGTTGTAACCAATCAGGAACTGCCAATGGCTGGAAGCTAACTGGTGCCCGGGAAGCATTAAATCTTCTCAAGGCCACCAATGGTTTCCACGATGACAGCCTTATTGGCTCGGGTGGTTTTGGTGATGTATACAAGGCCCAGTTGAAAGATGGCAGCACTGTAGCCATAAAGAAACTAATACATATCAGCGGACAGGGTGATCGTGAATTCACTGCCGAAATGGAAACAATAGGGACGATCAAGCACGGGAACCTTGTACCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTGGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACCGAAGAAAGCTGGCATCAAGTTAAACTGGGCTGCAAGGAGGAAGATTGCCATTGGGTCCGCGAGGGGACTGGCCTTTCTTCACCACAATTGTGTCCCACACATCATTCACAGGGATATGAAATCGAGCAACGTGCTGGTTGATGAAAATTTGGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTACAATGGACACCCATTTGAGTGTGAGCACTCTTGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGCTCCACGAAAGGCGATGTTTATAGTTATGGAGTAGTATTGCTTGAGCTGCTAACAGGAAAACGTCCTACAGATTCAGCAGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCTAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGTGAGCCTTGAAATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGACGATAGGCCATGGCGGCGTCCAACAATGATCGAAGTGATGGCAATGTTCAAGGAAATTCAAGCAGGGTCTGGGATGGACTCCCAATCAACCATAGCCACCGAGGACGGAGGTTTTGGCGTAGTTGAAATGGTAGAGATGAGCATAAAAGAAGTCCCAGAAAGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1084

Amino Acids

116.68

Weight (kDa)

5.67

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 113 - 170 1.6e-06 Leucine rich repeat
LRR_8 PF13855 136 - 194 7.6e-07 Leucine rich repeat
LRR_8 PF13855 205 - 265 3e-06 Leucine rich repeat
LRR_14 PF23598 346 - 498 3.3e-06 Leucine-rich repeat region
LRR_8 PF13855 401 - 459 9.1e-07 Leucine rich repeat
LRR_8 PF13855 425 - 484 7.6e-07 Leucine rich repeat
Island PF20141 497 - 561 2.6e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 590 - 648 1.1e-07 Leucine rich repeat
Pkinase PF00069 771 - 1041 4.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 775 - 1005 1.9e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 407
AccB1I GGYRCC 2 cut(s) 2270, 2807
AccB7I CCANNNNNTGG 1 cut(s) 2256
AccI GTMKAC 2 cut(s) 1653, 2355
AccII CGCG 4 cut(s) 528, 1137, 1874, 2636
AccIII TCCGGA 2 cut(s) 1487, 3004
AclI AACGTT 1 cut(s) 1676
AclWI GGATC 9 cut(s) 75, 88, 323, 930, 968, 981, 1440, 1601, 2996
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 6 cut(s) 1504, 1573, 1780, 2432, 2722, 3135
AcuI CTGAAG 1 cut(s) 989
AcyI GRCGYC 2 cut(s) 150, 3097
AfaI GTAC 7 cut(s) 877, 1001, 1193, 1763, 2484, 2537, 2770
AfiI CCNNNNNNNGG 7 cut(s) 461, 636, 1403, 1964, 2184, 2256, 2492
AflIII ACRYGT 1 cut(s) 1446
AjiI CACGTC 1 cut(s) 3025
AjnI CCWGG 4 cut(s) 261, 976, 2100, 2812
AleI CACNNNNGTG 1 cut(s) 2664
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
Alw21I GWGCWC 6 cut(s) 417, 702, 752, 1404, 2798, 3012
Alw26I GTCTC 5 cut(s) 397, 853, 1044, 1970, 2743
AlwI GGATC 9 cut(s) 75, 88, 323, 930, 968, 981, 1440, 1601, 2996
AlwNI CAGNNNCTG 4 cut(s) 1186, 1609, 2246, 3149
Ama87I CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
Aor13HI TCCGGA 2 cut(s) 1487, 3004
ApeKI GCWGC 7 cut(s) 90, 402, 547, 1606, 2379, 2606, 2899
ApoI RAATTY 6 cut(s) 1504, 1573, 1780, 2432, 2722, 3135
ArsI GACNNNNNNTTYG 2 cut(s) 3176, 3208
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 530, 1474
AspS9I GGNCC 8 cut(s) 4, 137, 296, 481, 587, 2054, 2362, 2631
AsuC2I CCSGG 5 cut(s) 78, 1323, 1324, 2275, 2276
AsuHPI GGTGA 6 cut(s) 94, 1325, 1418, 2360, 2435, 2648
AvaI CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
AvaII GGWCC 3 cut(s) 481, 587, 2631
BaeGI GKGCMC 1 cut(s) 2275
BamHI GGATCC 2 cut(s) 80, 973
BanI GGYRCC 2 cut(s) 2270, 2807
BanII GRGCYC 4 cut(s) 702, 752, 1404, 3012
BarI GAAGNNNNNNTAC 2 cut(s) 983, 1015
BauI CACGAG 1 cut(s) 2951
Bbv12I GWGCWC 6 cut(s) 417, 702, 752, 1404, 2798, 3012
BbvI GCAGC 7 cut(s) 102, 414, 559, 1618, 2391, 2593, 2886
BccI CCATC 8 cut(s) 65, 320, 1259, 1538, 2091, 2369, 3112, 3148
BceAI ACGGC 1 cut(s) 2534
BcgI CGANNNNNNTGC 4 cut(s) 1852, 1886, 2689, 2723
BciT130I CCWGG 4 cut(s) 263, 978, 2102, 2814
BciVI GTATCC 1 cut(s) 2489
BclI TGATCA 1 cut(s) 1143
BcnI CCSGG 5 cut(s) 78, 1323, 1324, 2275, 2276
BcoDI GTCTC 5 cut(s) 397, 853, 1044, 1970, 2743
BfaI CTAG 2 cut(s) 1349, 1860
BfmI CTRYAG 3 cut(s) 400, 2385, 2919
BfuAI ACCTGC 1 cut(s) 407
BfuI GTATCC 1 cut(s) 2489
BglI GCCNNNNNGGC 3 cut(s) 2019, 2331, 3094
BglII AGATCT 1 cut(s) 112
Bme1390I CCNGG 9 cut(s) 78, 263, 978, 1323, 1324, 2102, 2275, 2276, 2814
Bme18I GGWCC 3 cut(s) 481, 587, 2631
BmeT110I CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
BmgBI CACGTC 1 cut(s) 3025
BmgT120I GGNCC 8 cut(s) 4, 137, 296, 481, 587, 2054, 2362, 2631
BmrFI CCNGG 9 cut(s) 78, 263, 978, 1323, 1324, 2102, 2275, 2276, 2814
BmrI ACTGGG 3 cut(s) 79, 2359, 2611
BmsI GCATC 5 cut(s) 730, 2105, 2154, 2598, 2840
BmuI ACTGGG 3 cut(s) 79, 2359, 2611
BplI GAGNNNNNCTC 2 cut(s) 1650, 1682
BpmI CTGGAG 1 cut(s) 431
BpuEI CTTGAG 6 cut(s) 1076, 1226, 1343, 1800, 2279, 2915
BpuMI CCSGG 5 cut(s) 78, 1323, 1324, 2275, 2276
Bsa29I ATCGAT 1 cut(s) 1923
BsaBI GATNNNNATC 1 cut(s) 1250
BsaHI GRCGYC 2 cut(s) 150, 3097
BsaWI WCCGGW 3 cut(s) 1487, 1730, 3004
Bsc4I CCNNNNNNNGG 7 cut(s) 461, 636, 1403, 1964, 2184, 2256, 2492
Bse118I RCCGGY 2 cut(s) 1022, 1888
Bse1I ACTGG 7 cut(s) 85, 596, 798, 2272, 2365, 2606, 2649
Bse3DI GCAATG 2 cut(s) 1494, 3129
Bse8I GATNNNNATC 1 cut(s) 1250
BseAI TCCGGA 2 cut(s) 1487, 3004
BseBI CCWGG 4 cut(s) 263, 978, 2102, 2814
BseCI ATCGAT 1 cut(s) 1923
BseGI GGATG 3 cut(s) 1101, 2096, 3159
BseJI GATNNNNATC 1 cut(s) 1250
BseLI CCNNNNNNNGG 7 cut(s) 461, 636, 1403, 1964, 2184, 2256, 2492
BseMI GCAATG 2 cut(s) 1494, 3129
BseMII CTCAG 8 cut(s) 940, 1034, 1070, 1154, 1378, 1616, 1886, 1908
BseNI ACTGG 7 cut(s) 85, 596, 798, 2272, 2365, 2606, 2649
BseRI GAGGAG 4 cut(s) 89, 190, 223, 526
BseSI GKGCMC 1 cut(s) 2275
BseXI GCAGC 7 cut(s) 102, 414, 559, 1618, 2391, 2593, 2886
BseYI CCCAGC 2 cut(s) 955, 959
BsgI GTGCAG 3 cut(s) 109, 705, 1252
Bsh1236I CGCG 4 cut(s) 528, 1137, 1874, 2636
BshNI GGYRCC 2 cut(s) 2270, 2807
BshVI ATCGAT 1 cut(s) 1923
BsiHKAI GWGCWC 6 cut(s) 417, 702, 752, 1404, 2798, 3012
BsiHKCI CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
BslI CCNNNNNNNGG 7 cut(s) 461, 636, 1403, 1964, 2184, 2256, 2492
BsmAI GTCTC 5 cut(s) 397, 853, 1044, 1970, 2743
BsmBI CGTCTC 2 cut(s) 1044, 1970
BsmI GAATGC 1 cut(s) 1737
BsoBI CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
Bsp1286I GDGCHC 7 cut(s) 417, 702, 752, 1404, 2275, 2798, 3012
Bsp13I TCCGGA 2 cut(s) 1487, 3004
Bsp19I CCATGG 2 cut(s) 1561, 3089
Bsp68I TCGCGA 1 cut(s) 1137
BspCNI CTCAG 8 cut(s) 939, 1035, 1069, 1155, 1377, 1615, 1887, 1909
BspDI ATCGAT 1 cut(s) 1923
BspEI TCCGGA 2 cut(s) 1487, 3004
BspFNI CGCG 4 cut(s) 528, 1137, 1874, 2636
BspHI TCATGA 1 cut(s) 3012
BspMAI CTGCAG 1 cut(s) 404
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 9 cut(s) 75, 88, 323, 930, 968, 981, 1440, 1601, 2996
BspT107I GGYRCC 2 cut(s) 2270, 2807
BsrDI GCAATG 2 cut(s) 1494, 3129
BsrFI RCCGGY 2 cut(s) 1022, 1888
BsrI ACTGG 7 cut(s) 85, 596, 798, 2272, 2365, 2606, 2649
BssAI RCCGGY 2 cut(s) 1022, 1888
BssNAI GTATAC 2 cut(s) 1654, 2356
BssNI GRCGYC 2 cut(s) 150, 3097
BssSI CACGAG 1 cut(s) 2951
BssT1I CCWWGG 6 cut(s) 311, 631, 1195, 1561, 2491, 3089
Bst1107I GTATAC 2 cut(s) 1654, 2356
Bst2BI CACGAG 1 cut(s) 2951
Bst2UI CCWGG 4 cut(s) 263, 978, 2102, 2814
Bst4CI ACNGT 8 cut(s) 259, 587, 614, 1052, 1412, 1691, 2228, 2386
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 2 cut(s) 150, 3097
BstAPI GCANNNNNTGC 1 cut(s) 1557
BstC8I GCNNGC 8 cut(s) 172, 407, 545, 831, 2107, 2587, 2806, 3245
BstDSI CCRYGG 2 cut(s) 1561, 3089
BstF5I GGATG 3 cut(s) 1101, 2096, 3159
BstFNI CGCG 4 cut(s) 528, 1137, 1874, 2636
BstHHI GCGC 2 cut(s) 530, 1474
BstMAI GTCTC 5 cut(s) 397, 853, 1044, 1970, 2743
BstNI CCWGG 4 cut(s) 263, 978, 2102, 2814
BstNSI RCATGY 2 cut(s) 1450, 1762
BstSCI CCNGG 9 cut(s) 76, 261, 976, 1321, 1322, 2100, 2273, 2274, 2812
BstSFI CTRYAG 3 cut(s) 400, 2385, 2919
BstSLI GKGCMC 1 cut(s) 2275
BstUI CGCG 4 cut(s) 528, 1137, 1874, 2636
BstV1I GCAGC 7 cut(s) 102, 414, 559, 1618, 2391, 2593, 2886
BstX2I RGATCY 6 cut(s) 80, 112, 315, 922, 973, 1432
BstXI CCANNNNNNTGG 1 cut(s) 1166
BstYI RGATCY 6 cut(s) 80, 112, 315, 922, 973, 1432
BstZ17I GTATAC 2 cut(s) 1654, 2356
Bsu15I ATCGAT 1 cut(s) 1923
BsuI GTATCC 1 cut(s) 2489
BsuTUI ATCGAT 1 cut(s) 1923
BtgI CCRYGG 2 cut(s) 1561, 3089
BtgZI GCGATG 2 cut(s) 2116, 2880
BtrI CACGTC 1 cut(s) 3025
BtsCI GGATG 3 cut(s) 1101, 2096, 3159
BtsI GCAGTG 3 cut(s) 1506, 2116, 2436
BtsIMutI CAGTG 6 cut(s) 92, 805, 1506, 2116, 2382, 2436
BtuMI TCGCGA 1 cut(s) 1137
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 8 cut(s) 172, 407, 545, 831, 2107, 2587, 2806, 3245
CaiI CAGNNNCTG 4 cut(s) 1186, 1609, 2246, 3149
CciI TCATGA 1 cut(s) 3012
CfoI GCGC 2 cut(s) 530, 1474
Cfr10I RCCGGY 2 cut(s) 1022, 1888
Cfr13I GGNCC 8 cut(s) 4, 137, 296, 481, 587, 2054, 2362, 2631
Cfr9I CCCGGG 2 cut(s) 1322, 2274
ClaI ATCGAT 1 cut(s) 1923
CpoI CGGWCCG 1 cut(s) 587
CseI GACGC 1 cut(s) 3086
Csp6I GTAC 7 cut(s) 876, 1000, 1192, 1762, 2483, 2536, 2769
CspCI CAANNNNNGTGG 2 cut(s) 1291, 1326
CspI CGGWCCG 1 cut(s) 587
CviAII CATG 8 cut(s) 601, 1447, 1562, 1759, 2539, 2570, 3013, 3090
CviQI GTAC 7 cut(s) 876, 1000, 1192, 1762, 2483, 2536, 2769
DraI TTTAAA 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 4 cut(s) 700, 750, 1402, 3010
Eco130I CCWWGG 6 cut(s) 311, 631, 1195, 1561, 2491, 3089
Eco24I GRGCYC 4 cut(s) 702, 752, 1404, 3012
Eco47I GGWCC 3 cut(s) 481, 587, 2631
Eco53kI GAGCTC 4 cut(s) 700, 750, 1402, 3010
Eco57I CTGAAG 1 cut(s) 989
Eco88I CYCGRG 4 cut(s) 1322, 1580, 2274, 2335
EcoICRI GAGCTC 4 cut(s) 700, 750, 1402, 3010
EcoO109I RGGNCCY 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 2432
EcoRII CCWGG 4 cut(s) 261, 976, 2100, 2812
EcoT14I CCWWGG 6 cut(s) 311, 631, 1195, 1561, 2491, 3089
EcoT22I ATGCAT 1 cut(s) 745
EcoT38I GRGCYC 4 cut(s) 702, 752, 1404, 3012
ErhI CCWWGG 6 cut(s) 311, 631, 1195, 1561, 2491, 3089
Esp3I CGTCTC 2 cut(s) 1044, 1970
FaeI CATG 8 cut(s) 604, 1450, 1565, 1762, 2542, 2573, 3016, 3093
FalI AAGNNNNNCTT 2 cut(s) 2505, 2537
FatI CATG 8 cut(s) 600, 1446, 1561, 1758, 2538, 2569, 3012, 3089
FauI CCCGC 3 cut(s) 962, 1582, 1867
FbaI TGATCA 1 cut(s) 1143
FblI GTMKAC 2 cut(s) 1653, 2355
FokI GGATG 3 cut(s) 1088, 2083, 3166
FriOI GRGCYC 4 cut(s) 702, 752, 1404, 3012
FspBI CTAG 2 cut(s) 1349, 1860
GlaI GCGC 2 cut(s) 529, 1473
GsaI CCCAGC 2 cut(s) 959, 963
GsuI CTGGAG 1 cut(s) 431
HgaI GACGC 1 cut(s) 3086
HhaI GCGC 2 cut(s) 530, 1474
Hin1I GRCGYC 2 cut(s) 150, 3097
Hin1II CATG 8 cut(s) 604, 1450, 1565, 1762, 2542, 2573, 3016, 3093
Hin6I GCGC 2 cut(s) 528, 1472
HinP1I GCGC 2 cut(s) 528, 1472
HindIII AAGCTT 3 cut(s) 1130, 1337, 2551
HphI GGTGA 6 cut(s) 94, 1325, 1418, 2360, 2435, 2648
Hpy166II GTNNAC 3 cut(s) 1020, 1654, 2356
Hpy188I TCNGA 8 cut(s) 148, 325, 591, 1008, 1044, 1598, 1896, 2743
Hpy8I GTNNAC 3 cut(s) 1020, 1654, 2356
Hpy99I CGWCG 4 cut(s) 152, 308, 521, 2091
HpyAV CCTTC 8 cut(s) 196, 253, 465, 985, 1013, 1116, 2191, 3052
HpyCH4III ACNGT 8 cut(s) 259, 587, 614, 1052, 1412, 1691, 2228, 2386
HpyCH4IV ACGT 9 cut(s) 150, 303, 519, 1523, 1676, 1979, 2706, 2914, 3024
HpySE526I ACGT 9 cut(s) 150, 303, 519, 1523, 1676, 1979, 2706, 2914, 3024
Hsp92I GRCGYC 2 cut(s) 150, 3097
Hsp92II CATG 8 cut(s) 604, 1450, 1565, 1762, 2542, 2573, 3016, 3093
HspAI GCGC 2 cut(s) 528, 1472
Kpn2I TCCGGA 2 cut(s) 1487, 3004
Ksp22I TGATCA 1 cut(s) 1143
Lsp1109I GCAGC 7 cut(s) 102, 414, 559, 1618, 2391, 2593, 2886
LweI GCATC 5 cut(s) 730, 2105, 2154, 2598, 2840
MaeI CTAG 2 cut(s) 1349, 1860
MaeII ACGT 9 cut(s) 150, 303, 519, 1523, 1676, 1979, 2706, 2914, 3024
MaeIII GTNAC 6 cut(s) 335, 1046, 1367, 1406, 2124, 2231
MboII GAAGA 6 cut(s) 443, 2282, 2525, 2590, 2629, 2645
MfeI CAATTG 2 cut(s) 2058, 2662
MflI RGATCY 6 cut(s) 80, 112, 315, 922, 973, 1432
MhlI GDGCHC 7 cut(s) 417, 702, 752, 1404, 2275, 2798, 3012
MlyI GAGTC 5 cut(s) 5, 776, 1970, 2745, 3152
MmeI TCCRAC 7 cut(s) 171, 456, 744, 957, 1410, 1693, 3125
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 2 cut(s) 1487, 3004
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 7 cut(s) 356, 671, 839, 1680, 1842, 2285, 2597
MslI CAYNNNNRTG 3 cut(s) 1499, 2664, 2787
MspA1I CMGCKG 5 cut(s) 959, 987, 1162, 1609, 2415
MspR9I CCNGG 9 cut(s) 78, 263, 978, 1323, 1324, 2102, 2275, 2276, 2814
MunI CAATTG 2 cut(s) 2058, 2662
Mva1269I GAATGC 1 cut(s) 1737
MvaI CCWGG 4 cut(s) 263, 978, 2102, 2814
MvnI CGCG 4 cut(s) 528, 1137, 1874, 2636
NciI CCSGG 5 cut(s) 78, 1323, 1324, 2275, 2276
NcoI CCATGG 2 cut(s) 1561, 3089
NlaIII CATG 8 cut(s) 604, 1450, 1565, 1762, 2542, 2573, 3016, 3093
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 1 cut(s) 1406
NruI TCGCGA 1 cut(s) 1137
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 2 cut(s) 1450, 1762
OliI CACNNNNGTG 1 cut(s) 2664
PaeR7I CTCGAG 1 cut(s) 1580
PagI TCATGA 1 cut(s) 3012
PaqCI CACCTGC 1 cut(s) 407
PciI ACATGT 1 cut(s) 1446
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PctI GAATGC 1 cut(s) 1737
PdmI GAANNNNTTC 1 cut(s) 578
PflMI CCANNNNNTGG 1 cut(s) 2256
PfoI TCCNGGA 1 cut(s) 76
PleI GAGTC 5 cut(s) 5, 775, 1969, 2744, 3152
PpsI GAGTC 5 cut(s) 5, 775, 1969, 2744, 3152
PscI ACATGT 1 cut(s) 1446
Psp124BI GAGCTC 4 cut(s) 702, 752, 1404, 3012
Psp1406I AACGTT 1 cut(s) 1676
Psp6I CCWGG 4 cut(s) 261, 976, 2100, 2812
PspFI CCCAGC 2 cut(s) 955, 959
PspGI CCWGG 4 cut(s) 261, 976, 2100, 2812
PspPI GGNCC 8 cut(s) 4, 137, 296, 481, 587, 2054, 2362, 2631
PspXI VCTCGAGB 1 cut(s) 1580
PstI CTGCAG 1 cut(s) 404
PstNI CAGNNNCTG 4 cut(s) 1186, 1609, 2246, 3149
PsuI RGATCY 6 cut(s) 80, 112, 315, 922, 973, 1432
PvuII CAGCTG 4 cut(s) 959, 987, 1162, 1609
RruI TCGCGA 1 cut(s) 1137
RsaI GTAC 7 cut(s) 877, 1001, 1193, 1763, 2484, 2537, 2770
RsaNI GTAC 7 cut(s) 876, 1000, 1192, 1762, 2483, 2536, 2769
RseI CAYNNNNRTG 3 cut(s) 1499, 2664, 2787
Rsr2I CGGWCCG 1 cut(s) 587
RsrII CGGWCCG 1 cut(s) 587
SacI GAGCTC 4 cut(s) 702, 752, 1404, 3012
SaqAI TTAA 7 cut(s) 356, 671, 839, 1680, 1842, 2285, 2597
Sau96I GGNCC 8 cut(s) 4, 137, 296, 481, 587, 2054, 2362, 2631
SchI GAGTC 5 cut(s) 5, 776, 1970, 2745, 3152
ScrFI CCNGG 9 cut(s) 78, 263, 978, 1323, 1324, 2102, 2275, 2276, 2814
SduI GDGCHC 7 cut(s) 417, 702, 752, 1404, 2275, 2798, 3012
SfaNI GCATC 5 cut(s) 730, 2105, 2154, 2598, 2840
SfcI CTRYAG 3 cut(s) 400, 2385, 2919
Sfr274I CTCGAG 1 cut(s) 1580
SinI GGWCC 3 cut(s) 481, 587, 2631
SlaI CTCGAG 1 cut(s) 1580
SmaI CCCGGG 2 cut(s) 1324, 2276
SmiMI CAYNNNNRTG 3 cut(s) 1499, 2664, 2787
SmlI CTYRAG 7 cut(s) 1091, 1205, 1358, 1580, 1815, 2294, 2894
SmoI CTYRAG 7 cut(s) 1091, 1205, 1358, 1580, 1815, 2294, 2894
SspMI CTAG 2 cut(s) 1349, 1860
SstI GAGCTC 4 cut(s) 702, 752, 1404, 3012
StyD4I CCNGG 9 cut(s) 76, 261, 976, 1321, 1322, 2100, 2273, 2274, 2812
StyI CCWWGG 6 cut(s) 311, 631, 1195, 1561, 2491, 3089
TaaI ACNGT 8 cut(s) 259, 587, 614, 1052, 1412, 1691, 2228, 2386
TaiI ACGT 9 cut(s) 153, 306, 522, 1526, 1679, 1982, 2709, 2917, 3027
TaqII GACCGA 1 cut(s) 1244
TatI WGTACW 3 cut(s) 1761, 2535, 2768
TauI GCSGC 4 cut(s) 167, 170, 528, 3097
Tru1I TTAA 7 cut(s) 356, 671, 839, 1680, 1842, 2285, 2597
Tru9I TTAA 7 cut(s) 356, 671, 839, 1680, 1842, 2285, 2597
TscAI CASTG 6 cut(s) 92, 805, 1506, 2116, 2389, 2443
TseFI GTSAC 1 cut(s) 1406
TseI GCWGC 7 cut(s) 90, 402, 547, 1606, 2379, 2606, 2899
Tsp45I GTSAC 1 cut(s) 1406
TspDTI ATGAA 8 cut(s) 587, 839, 1278, 2555, 2586, 2708, 2733, 3029
TspGWI ACGGA 2 cut(s) 1026, 3201
TspMI CCCGGG 2 cut(s) 1322, 2274
TspRI CASTG 6 cut(s) 92, 805, 1506, 2116, 2389, 2443
Van91I CCANNNNNTGG 1 cut(s) 2256
VpaK11BI GGWCC 3 cut(s) 481, 587, 2631
XapI RAATTY 6 cut(s) 1504, 1573, 1780, 2432, 2722, 3135
XceI RCATGY 2 cut(s) 1450, 1762
XcmI CCANNNNNNNNNTGG 1 cut(s) 2372
XhoI CTCGAG 1 cut(s) 1580
XmaI CCCGGG 2 cut(s) 1322, 2274
XmiI GTMKAC 2 cut(s) 1653, 2355
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 2 cut(s) 1349, 1860
ZraI GACGTC 1 cut(s) 151
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.