pycom01g05820

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011740
Physical Location & Seq
Forward (+)
5704819 .. 5707185
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05820.1

Sequence Viewer

Length: 846 bp
ATGGGTAGGAACATTTCTGACTTTTGCCGGCTTCGTCGTGGTTGGCGGTTTCTCCACGCCTCGCTATATATAAATACGTTCAACTCAAAAAATGGAAACCAGAAAAAAAACCAAATTTGGAAAAAAGAAAGAAAGCGTAGAGTCAGATCCAAAATAGCCGTGGGTAGAAAACGAAAACGAATCGAGGGAGCTGAAACAAAATCAATCGAATCAATTTTGAAAATGGCGACGGTGTCCCCTCTGGCCAAATACAAGCTCGTCTTCCTGGGCGATCAGTCCGTCGGCAAAACCAGCATCATCACCCGCTTCATGTACGACAAATTCGACACCACGTATCAGGCTACGATTGGAATCGATTTTCTGTCAAAAACGATGTACCTTGAAGATCGGACAGTTCGTTTGCAGCTCTGGGATACTGCAGGCCAAGAAAGATTTAGGAGTCTGATTCCAAGCTACATCAGGGATTCTTCTGTTGCAGTAATCGTCTATGATGTAGCCAATAGACAGTCATTTCTGAACACTAATAAGTGGATTGAGGAAGTACGAACAGAACGGGGAACTGATGTCATCATCGTGCTTGTGGGGAACAAGACTGATCTCGTCGATAAAAGGCAAGTCTCGATAGAGGAAGGAGATTCAAAATCTCGCGAGTTTGGAGTCATGTTCATTGAAACTAGTGCAAAAGCAGGATTTAACATCAAGCCTCTATTCCGTAAAATTGCTGCTGCTTTGCCAGGGATGGAAACCCTTTCTTCCACAAAACAGGAAGACATGGTCGATGTAAACCTGAAACCGACTGTTAACTCGTCCCATACAGAGCAGCAAGGAGGAGGTTGCGCATGCTAG

Protein Analysis

282

Amino Acids

32.07

Weight (kDa)

9.9

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 82 - 204 1.8e-14 ADP-ribosylation factor family
Ras PF00071 85 - 243 4.5e-57 Ras family
Roc PF08477 85 - 199 2.6e-37 Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU PF00009 117 - 245 3.3e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011740)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22290 AT4G39890 AT5G10260
fragaria_vesca FvH4_1g20860
malus_domestica MD01G1030600.v1.1 MD15G1323700.v1.1
prunus_persica Prupe.6G187300_v2.0.a1
pyrus_communis pycom01g05820 pycom15g28560
rosa_chinensis RchiOBHm_Chr2g0113471
rosa_laevigata RLG00000018067
rosa_roxburghii Rroxscaffold_2G00129220 Rroxscaffold_2G00129950
rosa_rugosa Rorug02G0187700
rosa_samantha Rh2AG242200 Rh2BG256200 Rh2DG250200
rosa_wichuraiana Rw0G018870 Rw2G018930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 838
AccII CGCG 1 cut(s) 648
AciI CCGC 2 cut(s) 46, 304
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 2 cut(s) 114, 320
AfaI GTAC 3 cut(s) 314, 377, 543
AgsI TTSAA 5 cut(s) 82, 220, 383, 639, 671
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 2 cut(s) 264, 733
AluBI AGCT 4 cut(s) 191, 256, 406, 453
AluI AGCT 4 cut(s) 191, 256, 406, 453
Alw26I GTCTC 1 cut(s) 622
AlwI GGATC 1 cut(s) 141
AoxI GGCC 2 cut(s) 243, 421
ApeKI GCWGC 4 cut(s) 403, 722, 725, 820
ApoI RAATTY 2 cut(s) 114, 320
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
AspLEI GCGC 1 cut(s) 839
AsuHPI GGTGA 1 cut(s) 292
BalI TGGCCA 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 253, 774
BbvI GCAGC 4 cut(s) 415, 709, 712, 832
BccI CCATC 1 cut(s) 733
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 266, 735
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 622
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 2 cut(s) 675, 844
BfmI CTRYAG 1 cut(s) 417
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 4 cut(s) 404, 723, 726, 821
BlsI GCNGC 4 cut(s) 405, 724, 727, 822
Bme1390I CCNGG 2 cut(s) 266, 735
BmrFI CCNGG 2 cut(s) 266, 735
BmsI GCATC 1 cut(s) 303
BpiI GAAGAC 2 cut(s) 253, 774
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 159, 265, 734
Bse118I RCCGGY 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 2 cut(s) 266, 735
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 159, 265, 734
BseGI GGATG 1 cut(s) 744
BseJI GATNNNNATC 1 cut(s) 350
BseRI GAGGAG 1 cut(s) 843
BseXI GCAGC 4 cut(s) 415, 709, 712, 832
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 2 cut(s) 245, 423
BshVI ATCGAT 1 cut(s) 354
BsiSI CCGG 1 cut(s) 28
BslFI GGGAC 2 cut(s) 220, 793
BsmAI GTCTC 1 cut(s) 622
BsmFI GGGAC 2 cut(s) 220, 793
BsnI GGCC 2 cut(s) 245, 423
Bsp143I GATC 4 cut(s) 146, 271, 385, 595
Bsp68I TCGCGA 1 cut(s) 648
BspACI CCGC 2 cut(s) 46, 304
BspANI GGCC 2 cut(s) 245, 423
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 1 cut(s) 648
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 27
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 3 cut(s) 159, 265, 734
BssMI GATC 4 cut(s) 146, 271, 385, 595
Bst2UI CCWGG 2 cut(s) 266, 735
Bst4CI ACNGT 4 cut(s) 232, 394, 507, 799
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 29, 421, 841
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 744
BstFNI CGCG 1 cut(s) 648
BstHHI GCGC 1 cut(s) 839
BstKTI GATC 4 cut(s) 149, 274, 388, 598
BstMAI GTCTC 1 cut(s) 622
BstMBI GATC 4 cut(s) 146, 271, 385, 595
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 2 cut(s) 266, 735
BstNSI RCATGY 1 cut(s) 843
BstSCI CCNGG 2 cut(s) 264, 733
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 4 cut(s) 415, 709, 712, 832
BstV2I GAAGAC 2 cut(s) 253, 774
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 2 cut(s) 245, 423
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 1 cut(s) 744
BtuMI TCGCGA 1 cut(s) 648
Cac8I GCNNGC 3 cut(s) 29, 421, 841
CfoI GCGC 1 cut(s) 839
Cfr10I RCCGGY 1 cut(s) 27
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 3 cut(s) 313, 376, 542
CviAII CATG 4 cut(s) 310, 661, 772, 840
CviQI GTAC 3 cut(s) 313, 376, 542
DpnI GATC 4 cut(s) 148, 273, 387, 597
DpnII GATC 4 cut(s) 146, 271, 385, 595
EaeI YGGCCR 1 cut(s) 243
EcoRII CCWGG 2 cut(s) 264, 733
FaeI CATG 4 cut(s) 313, 664, 775, 843
FaiI YATR 9 cut(s) 67, 69, 71, 311, 489, 662, 773, 813, 841
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 220, 793
FatI CATG 4 cut(s) 309, 660, 771, 839
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 4 cut(s) 404, 723, 726, 821
FokI GGATG 1 cut(s) 751
Fsp4HI GCNGC 4 cut(s) 404, 723, 726, 821
FspBI CTAG 2 cut(s) 675, 844
FspI TGCGCA 1 cut(s) 838
GlaI GCGC 1 cut(s) 838
GluI GCNGC 4 cut(s) 404, 723, 726, 821
HaeIII GGCC 2 cut(s) 245, 423
HapII CCGG 1 cut(s) 28
HhaI GCGC 1 cut(s) 839
Hin1II CATG 4 cut(s) 313, 664, 775, 843
Hin6I GCGC 1 cut(s) 837
HinP1I GCGC 1 cut(s) 837
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 9 cut(s) 141, 180, 209, 351, 439, 445, 464, 635, 657
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 1 cut(s) 28
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 784, 802
Hpy188I TCNGA 5 cut(s) 19, 146, 390, 444, 516
Hpy188III TCNNGA 2 cut(s) 619, 647
Hpy8I GTNNAC 2 cut(s) 784, 802
Hpy99I CGWCG 4 cut(s) 39, 232, 284, 605
HpyAV CCTTC 1 cut(s) 623
HpyCH4III ACNGT 4 cut(s) 232, 394, 507, 799
HpyCH4IV ACGT 2 cut(s) 77, 332
HpyCH4V TGCA 4 cut(s) 403, 419, 476, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpySE526I ACGT 2 cut(s) 77, 332
Hsp92II CATG 4 cut(s) 313, 664, 775, 843
HspAI GCGC 1 cut(s) 837
KroI GCCGGC 1 cut(s) 27
KroNI GCCGGC 1 cut(s) 29
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 4 cut(s) 146, 271, 385, 595
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 4 cut(s) 415, 709, 712, 832
LweI GCATC 1 cut(s) 303
MaeI CTAG 2 cut(s) 675, 844
MaeII ACGT 2 cut(s) 77, 332
MalI GATC 4 cut(s) 148, 273, 387, 597
MboI GATC 4 cut(s) 146, 271, 385, 595
MboII GAAGA 5 cut(s) 253, 395, 459, 744, 779
MflI RGATCY 1 cut(s) 146
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 4 cut(s) 114, 213, 320, 717
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 3 cut(s) 150, 448, 666
MnlI CCTC 8 cut(s) 70, 178, 249, 529, 619, 714, 821, 824
Mox20I TGGCCA 1 cut(s) 245
MroNI GCCGGC 1 cut(s) 27
MscI TGGCCA 1 cut(s) 245
MseI TTAA 2 cut(s) 693, 801
MslI CAYNNNNRTG 1 cut(s) 572
Msp20I TGGCCA 1 cut(s) 245
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 2 cut(s) 266, 735
MvaI CCWGG 2 cut(s) 266, 735
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 1 cut(s) 291
NaeI GCCGGC 1 cut(s) 29
NdeII GATC 4 cut(s) 146, 271, 385, 595
NgoMIV GCCGGC 1 cut(s) 27
NlaIII CATG 4 cut(s) 313, 664, 775, 843
NruI TCGCGA 1 cut(s) 648
NsbI TGCGCA 1 cut(s) 838
NspI RCATGY 1 cut(s) 843
PaeI GCATGC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 2 cut(s) 321, 394
PdiI GCCGGC 1 cut(s) 29
PfeI GAWTC 6 cut(s) 180, 209, 351, 445, 464, 635
PflFI GACNNNGTC 2 cut(s) 232, 773
PkrI GCNGC 4 cut(s) 405, 724, 727, 822
PleI GAGTC 3 cut(s) 149, 447, 665
PpsI GAGTC 3 cut(s) 149, 447, 665
Ppu21I YACGTR 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 264, 733
PspGI CCWGG 2 cut(s) 264, 733
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 2 cut(s) 232, 773
RruI TCGCGA 1 cut(s) 648
RsaI GTAC 3 cut(s) 314, 377, 543
RsaNI GTAC 3 cut(s) 313, 376, 542
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 2 cut(s) 693, 801
SatI GCNGC 4 cut(s) 404, 723, 726, 821
Sau3AI GATC 4 cut(s) 146, 271, 385, 595
SchI GAGTC 3 cut(s) 150, 448, 666
ScrFI CCNGG 2 cut(s) 266, 735
SetI ASST 9 cut(s) 80, 193, 258, 335, 381, 408, 455, 789, 835
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 572
SpeI ACTAGT 1 cut(s) 674
SphI GCATGC 1 cut(s) 843
Sse9I AATT 4 cut(s) 114, 213, 320, 717
SsiI CCGC 2 cut(s) 46, 304
SspMI CTAG 2 cut(s) 675, 844
StyD4I CCNGG 2 cut(s) 264, 733
TaaI ACNGT 4 cut(s) 232, 394, 507, 799
TaiI ACGT 2 cut(s) 80, 335
TaqI TCGA 7 cut(s) 183, 207, 324, 354, 603, 620, 777
TasI AATT 4 cut(s) 114, 213, 320, 717
TfiI GAWTC 6 cut(s) 180, 209, 351, 445, 464, 635
Tru1I TTAA 2 cut(s) 693, 801
Tru9I TTAA 2 cut(s) 693, 801
TseI GCWGC 4 cut(s) 403, 722, 725, 820
TspDTI ATGAA 2 cut(s) 298, 655
TspGWI ACGGA 2 cut(s) 268, 701
Tth111I GACNNNGTC 2 cut(s) 232, 773
XapI RAATTY 2 cut(s) 114, 320
XceI RCATGY 1 cut(s) 843
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XspI CTAG 2 cut(s) 675, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.