pycom01g06140
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009254
Physical Location & Seq
Reverse (-)
6245471 .. 6259526
14056 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
ATGGGAGGCGGCGGCGGCAGAGGCAGAGGCGGTTCTCGCACTCAGCGACGCCACTTCCGTCAGAACAGAGAGAACGTTTGGAAGCGTCCCAAGTCTGATCCTTCCTCTGAAAACACCAATGAAAACAATGGCGAGAATCGTGGTTGGGAACCCTTTGCCACTCAAAACCCTGCATTCGATGAATACTATAAGGAGCAAGGGATAGTGAGCCCAGAAGAGTGGGATGCGTTCATTGAAGTTCTTCGAAAGCCGTTGCCTGCTGCTTTTAGAATCAATTCAAGTAGCCAATTTTGTTCGGATATCCGAAGGCAGTTAGAGGATGACTTTATGATCTCTCTACAATCTGAGGTAAGTGAAGGGGGTGAACCACAGCCTATTAGGCCGTTGCCTTGGTATCCTGAGAATCTAGCTTGGCATTCGAATTTCTCTCGCATGCAGCTGAGGAAGAACCAAACACTTGAGAGGTTTCATGAGTTCTTAAAGCTTGAAAATGAGATTGGAAACATCACAAGACAGGAGGCTGTCAGCATGGTGCCTCCACTATTCCTTGATGTACGTCCAGATCATTTTGTACTTGATATGTGCGCTGCACCAGGTTCGAAAACATTCCAATTGCTTGAGATTATACACCGATCGATCAAACCAGGATCCCTACCTGATGGATTGGTTGTAGCAAATGATCGATCTGATGTCCAAAGATGTAATCTACTCATCCATCAAACAAAACGAATGTGCACAGCCAACTTGATAGTCACAAATCATGAGGCTCAGCACTTTCCTGGCTGCCGGGCAAAGTCTACTGCTTCCGAAATAGGAGCTGAAATGGAGCCACCCATCAGCCAACTTGTGTTTGATCGTGTTTTATGTGATGTTCCTTGCAGTGGAGATGGTACCCTTCGCAAGGCTCCTGATATATGGAGGAAATGGCATGCTGGATTGGGCAATGGGGTTCATCCTCTACAAATTCAAATAGCTATGCGAGGTTTATCATTGCTTAAAGTTGGTGGAAGAATGGTGTACTCTACCTGCTCTATGAATCCAGTTGAGAATGAAGCTGTGGTTGCTGAGTTTTTGCGGGGAGGTGGTGGGTTTGTTGAGCTTATTGATGTCTCTAGTGAGCTGCCTCAACTTGTTCGAAGGCCAGGTCTTAAAAAATGGAAGATACGTGACAAGGGTAGGTGGTTAGCTTCCCACAAAATGGTCAGCAAATATCGCAAAAGTGTCATTGTTCCCAGCATGTTTCCTTCTGGAAGAAGCTTTACAGAACAAATGGACGCTAATGGTAGTATGGAAGTGGGAGAAAAGCATGAGAATGGTGGAAATGTTGAAGATGCGTTGCAGTCAACTGATGATCCTGCAACTGTAGCCAATGAACAAGAGGAGGAAGTTTCTGATTTTCCACTAGAACGCTGCATGAGGATAGTCCCGGATCGATCAAAACGGAGGAGCTTTCTTCATTGCTGTCTTCCACAAACATTCTGCTTTGCCAGCCACTCAGGGGAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltr_RsmB-F

Protein Analysis

503

Amino Acids

56.63

Weight (kDa)

6.72

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltr_RsmB-F PF01189 182 - 359 8.7e-33 16S rRNA methyltransferase RsmB/F
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1034
Acc65I GGTACC 1 cut(s) 890
AccB1I GGYRCC 2 cut(s) 532, 890
AccB7I CCANNNNNTGG 1 cut(s) 1198
AccI GTMKAC 1 cut(s) 797
AciI CCGC 5 cut(s) 9, 12, 15, 30, 1075
AclI AACGTT 1 cut(s) 75
AclWI GGATC 5 cut(s) 92, 642, 655, 1346, 1437
AcsI RAATTY 2 cut(s) 421, 963
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 4 cut(s) 555, 573, 892, 1019
AfiI CCNNNNNNNGG 4 cut(s) 881, 901, 1198, 1498
AgsI TTSAA 5 cut(s) 236, 279, 488, 968, 1328
AjnI CCWGG 4 cut(s) 592, 643, 778, 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
Alw21I GWGCWC 1 cut(s) 737
Alw26I GTCTC 1 cut(s) 1114
Alw44I GTGCAC 1 cut(s) 733
AlwI GGATC 5 cut(s) 92, 642, 655, 1346, 1437
AoxI GGCC 2 cut(s) 380, 1139
ApaLI GTGCAC 1 cut(s) 733
ApeKI GCWGC 6 cut(s) 260, 436, 587, 783, 1120, 1410
ApoI RAATTY 2 cut(s) 421, 963
ArsI GACNNNNNNTTYG 2 cut(s) 1129, 1161
Asp700I GAANNNNTTC 3 cut(s) 240, 274, 605
Asp718I GGTACC 1 cut(s) 890
AspLEI GCGC 1 cut(s) 587
AsuC2I CCSGG 2 cut(s) 788, 1427
AsuHPI GGTGA 1 cut(s) 374
AsuII TTCGAA 4 cut(s) 244, 419, 599, 1135
BaeGI GKGCMC 1 cut(s) 737
BamHI GGATCC 1 cut(s) 647
BanI GGYRCC 2 cut(s) 532, 890
BanII GRGCYC 1 cut(s) 212
BarI GAAGNNNNNNTAC 2 cut(s) 1243, 1275
BbsI GAAGAC 1 cut(s) 1457
Bbv12I GWGCWC 1 cut(s) 737
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 6 cut(s) 247, 448, 574, 770, 1107, 1397
BccI CCATC 4 cut(s) 653, 723, 842, 881
BceAI ACGGC 2 cut(s) 235, 367
BcgI CGANNNNNNTGC 2 cut(s) 579, 613
BciT130I CCWGG 4 cut(s) 594, 645, 780, 1143
BciVI GTATCC 1 cut(s) 405
BcnI CCSGG 2 cut(s) 788, 1427
BcoDI GTCTC 1 cut(s) 1114
BfaI CTAG 4 cut(s) 407, 1113, 1403, 1507
BfmI CTRYAG 1 cut(s) 1362
BfuAI ACCTGC 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 379
BisI GCNGC 9 cut(s) 10, 13, 16, 261, 437, 588, 784, 1121, 1411
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 9 cut(s) 11, 14, 17, 262, 438, 589, 785, 1122, 1412
Bme1390I CCNGG 6 cut(s) 594, 645, 780, 788, 1143, 1427
BmiI GGNNCC 6 cut(s) 150, 534, 649, 828, 892, 906
BmrFI CCNGG 6 cut(s) 594, 645, 780, 788, 1143, 1427
BmsI GCATC 2 cut(s) 214, 1321
BpiI GAAGAC 1 cut(s) 1457
Bpu10I CCTNAGC 1 cut(s) 440
Bpu1102I GCTNAGC 1 cut(s) 768
Bpu14I TTCGAA 4 cut(s) 244, 419, 599, 1135
BpuEI CTTGAG 2 cut(s) 479, 638
BpuMI CCSGG 2 cut(s) 788, 1427
Bsa29I ATCGAT 3 cut(s) 635, 682, 1432
BsaAI YACGTR 1 cut(s) 1166
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 389
Bsc4I CCNNNNNNNGG 4 cut(s) 881, 901, 1198, 1498
Bse1I ACTGG 1 cut(s) 1040
Bse3DI GCAATG 3 cut(s) 949, 989, 1456
BseBI CCWGG 4 cut(s) 594, 645, 780, 1143
BseCI ATCGAT 3 cut(s) 635, 682, 1432
BseDI CCNNGG 1 cut(s) 389
BseGI GGATG 4 cut(s) 229, 325, 711, 952
BseLI CCNNNNNNNGG 4 cut(s) 881, 901, 1198, 1498
BseMI GCAATG 3 cut(s) 949, 989, 1456
BseMII CTCAG 7 cut(s) 56, 336, 390, 431, 782, 1056, 1509
BseNI ACTGG 1 cut(s) 1040
BseRI GAGGAG 2 cut(s) 1394, 1459
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 6 cut(s) 247, 448, 574, 770, 1107, 1397
BseYI CCCAGC 1 cut(s) 1232
BsgI GTGCAG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 635
BshFI GGCC 2 cut(s) 382, 1141
BshNI GGYRCC 2 cut(s) 532, 890
BshVI ATCGAT 3 cut(s) 635, 682, 1432
BsiEI CGRYCG 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 737
BsiSI CCGG 2 cut(s) 787, 1427
BslFI GGGAC 2 cut(s) 72, 1409
BslI CCNNNNNNNGG 4 cut(s) 881, 901, 1198, 1498
BsmAI GTCTC 1 cut(s) 1114
BsmFI GGGAC 2 cut(s) 72, 1409
BsmI GAATGC 2 cut(s) 173, 415
BsnI GGCC 2 cut(s) 382, 1141
Bsp119I TTCGAA 4 cut(s) 244, 419, 599, 1135
Bsp1286I GDGCHC 2 cut(s) 212, 737
Bsp1720I GCTNAGC 1 cut(s) 768
BspACI CCGC 5 cut(s) 9, 12, 15, 30, 1075
BspANI GGCC 2 cut(s) 382, 1141
BspCNI CTCAG 7 cut(s) 55, 337, 391, 432, 781, 1057, 1508
BspDI ATCGAT 3 cut(s) 635, 682, 1432
BspHI TCATGA 2 cut(s) 469, 760
BspLI GGNNCC 6 cut(s) 150, 534, 649, 828, 892, 906
BspMI ACCTGC 1 cut(s) 1034
BspPI GGATC 5 cut(s) 92, 642, 655, 1346, 1437
BspT104I TTCGAA 4 cut(s) 244, 419, 599, 1135
BspT107I GGYRCC 2 cut(s) 532, 890
BsrDI GCAATG 3 cut(s) 949, 989, 1456
BsrI ACTGG 1 cut(s) 1040
BssECI CCNNGG 1 cut(s) 389
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 4 cut(s) 594, 645, 780, 1143
Bst4CI ACNGT 1 cut(s) 1363
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 49
BstBAI YACGTR 1 cut(s) 1166
BstBI TTCGAA 4 cut(s) 244, 419, 599, 1135
BstC8I GCNNGC 4 cut(s) 258, 434, 930, 1489
BstDEI CTNAG 7 cut(s) 42, 345, 399, 440, 768, 1065, 1495
BstENI CCTNNNNNAGG 1 cut(s) 899
BstF5I GGATG 4 cut(s) 229, 325, 711, 952
BstHHI GCGC 1 cut(s) 587
BstMAI GTCTC 1 cut(s) 1114
BstMCI CGRYCG 1 cut(s) 635
BstMWI GCNNNNNNNGC 8 cut(s) 15, 21, 36, 379, 1061, 1212, 1364, 1488
BstNI CCWGG 4 cut(s) 594, 645, 780, 1143
BstNSI RCATGY 3 cut(s) 436, 932, 1240
BstSCI CCNGG 6 cut(s) 592, 643, 778, 786, 1141, 1425
BstSFI CTRYAG 1 cut(s) 1362
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 6 cut(s) 247, 448, 574, 770, 1107, 1397
BstV2I GAAGAC 1 cut(s) 1457
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 219
BstYI RGATCY 1 cut(s) 647
Bsu15I ATCGAT 3 cut(s) 635, 682, 1432
BsuI GTATCC 1 cut(s) 405
BsuRI GGCC 2 cut(s) 382, 1141
BsuTUI ATCGAT 3 cut(s) 635, 682, 1432
BtsCI GGATG 4 cut(s) 229, 325, 711, 952
BtsI GCAGTG 1 cut(s) 886
BtsIMutI CAGTG 1 cut(s) 886
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 4 cut(s) 258, 434, 930, 1489
CciI TCATGA 2 cut(s) 469, 760
CfoI GCGC 1 cut(s) 587
ClaI ATCGAT 3 cut(s) 635, 682, 1432
CseI GACGC 3 cut(s) 57, 74, 1283
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 4 cut(s) 554, 572, 891, 1018
CviAII CATG 8 cut(s) 433, 470, 529, 761, 929, 1237, 1307, 1414
CviQI GTAC 4 cut(s) 554, 572, 891, 1018
DdeI CTNAG 7 cut(s) 42, 345, 399, 440, 768, 1065, 1495
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 212
Eco32I GATATC 1 cut(s) 301
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRII CCWGG 4 cut(s) 592, 643, 778, 1141
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 389
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 8 cut(s) 436, 473, 532, 764, 932, 1240, 1310, 1417
FaqI GGGAC 2 cut(s) 72, 1409
FatI CATG 8 cut(s) 432, 469, 528, 760, 928, 1236, 1306, 1413
FauI CCCGC 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 9 cut(s) 10, 13, 16, 261, 437, 588, 784, 1121, 1411
FokI GGATG 4 cut(s) 236, 332, 698, 939
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 9 cut(s) 10, 13, 16, 261, 437, 588, 784, 1121, 1411
FspBI CTAG 4 cut(s) 407, 1113, 1403, 1507
GlaI GCGC 1 cut(s) 586
GluI GCNGC 9 cut(s) 10, 13, 16, 261, 437, 588, 784, 1121, 1411
GsaI CCCAGC 1 cut(s) 1236
HaeIII GGCC 2 cut(s) 382, 1141
HapII CCGG 2 cut(s) 787, 1427
HgaI GACGC 3 cut(s) 57, 74, 1283
HhaI GCGC 1 cut(s) 587
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 8 cut(s) 436, 473, 532, 764, 932, 1240, 1310, 1417
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HincII GTYRAC 1 cut(s) 1344
HindII GTYRAC 1 cut(s) 1344
HindIII AAGCTT 2 cut(s) 482, 1255
HinfI GANTC 4 cut(s) 136, 270, 403, 1036
HpaII CCGG 2 cut(s) 787, 1427
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 5 cut(s) 365, 735, 798, 1018, 1344
Hpy188I TCNGA 9 cut(s) 63, 97, 109, 298, 305, 346, 688, 808, 1393
Hpy188III TCNNGA 6 cut(s) 398, 470, 560, 761, 908, 1248
Hpy8I GTNNAC 5 cut(s) 365, 735, 798, 1018, 1344
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 6 cut(s) 111, 300, 350, 905, 1131, 1254
HpyCH4III ACNGT 1 cut(s) 1363
HpyCH4IV ACGT 3 cut(s) 75, 556, 1165
HpyCH4V TGCA 8 cut(s) 173, 436, 590, 735, 879, 1339, 1358, 1413
HpyF10VI GCNNNNNNNGC 8 cut(s) 15, 21, 36, 379, 1061, 1212, 1364, 1488
HpyF3I CTNAG 7 cut(s) 42, 345, 399, 440, 768, 1065, 1495
HpySE526I ACGT 3 cut(s) 75, 556, 1165
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 8 cut(s) 436, 473, 532, 764, 932, 1240, 1310, 1417
HspAI GCGC 1 cut(s) 585
KpnI GGTACC 1 cut(s) 894
LmnI GCTCC 5 cut(s) 193, 815, 826, 910, 1446
Lsp1109I GCAGC 6 cut(s) 247, 448, 574, 770, 1107, 1397
LweI GCATC 2 cut(s) 214, 1321
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 4 cut(s) 407, 1113, 1403, 1507
MaeII ACGT 3 cut(s) 75, 556, 1165
MaeIII GTNAC 2 cut(s) 751, 1166
MboII GAAGA 9 cut(s) 227, 233, 457, 1020, 1171, 1263, 1340, 1445, 1457
MfeI CAATTG 1 cut(s) 611
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 2 cut(s) 212, 737
MluCI AATT 5 cut(s) 274, 287, 421, 611, 963
MroXI GAANNNNTTC 3 cut(s) 240, 274, 605
MseI TTAA 3 cut(s) 479, 996, 1149
MslI CAYNNNNRTG 1 cut(s) 1311
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 2 cut(s) 787, 1427
MspR9I CCNGG 6 cut(s) 594, 645, 780, 788, 1143, 1427
MunI CAATTG 1 cut(s) 611
Mva1269I GAATGC 2 cut(s) 173, 415
MvaI CCWGG 4 cut(s) 594, 645, 780, 1143
MwoI GCNNNNNNNGC 8 cut(s) 15, 21, 36, 379, 1061, 1212, 1364, 1488
NciI CCSGG 2 cut(s) 788, 1427
NlaIII CATG 8 cut(s) 436, 473, 532, 764, 932, 1240, 1310, 1417
NlaIV GGNNCC 6 cut(s) 150, 534, 649, 828, 892, 906
NmuCI GTSAC 2 cut(s) 751, 1166
NspI RCATGY 3 cut(s) 436, 932, 1240
NspV TTCGAA 4 cut(s) 244, 419, 599, 1135
PaeI GCATGC 2 cut(s) 436, 932
PagI TCATGA 2 cut(s) 469, 760
PcsI WCGNNNNNNNCGW 2 cut(s) 43, 55
PctI GAATGC 2 cut(s) 173, 415
PdmI GAANNNNTTC 3 cut(s) 240, 274, 605
PfeI GAWTC 4 cut(s) 136, 270, 403, 1036
PflMI CCANNNNNTGG 1 cut(s) 1198
PfoI TCCNGGA 1 cut(s) 1425
PkrI GCNGC 9 cut(s) 11, 14, 17, 262, 438, 589, 785, 1122, 1412
Ple19I CGATCG 1 cut(s) 635
Ppu21I YACGTR 1 cut(s) 1166
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 4 cut(s) 592, 643, 778, 1141
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 4 cut(s) 592, 643, 778, 1141
PspN4I GGNNCC 6 cut(s) 150, 534, 649, 828, 892, 906
PsuI RGATCY 1 cut(s) 647
PvuI CGATCG 1 cut(s) 635
PvuII CAGCTG 1 cut(s) 439
RsaI GTAC 4 cut(s) 555, 573, 892, 1019
RsaNI GTAC 4 cut(s) 554, 572, 891, 1018
RseI CAYNNNNRTG 1 cut(s) 1311
SaqAI TTAA 3 cut(s) 479, 996, 1149
SatI GCNGC 9 cut(s) 10, 13, 16, 261, 437, 588, 784, 1121, 1411
ScrFI CCNGG 6 cut(s) 594, 645, 780, 788, 1143, 1427
SduI GDGCHC 2 cut(s) 212, 737
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 2 cut(s) 214, 1321
SfcI CTRYAG 1 cut(s) 1362
SfuI TTCGAA 4 cut(s) 244, 419, 599, 1135
SmiMI CAYNNNNRTG 1 cut(s) 1311
SmlI CTYRAG 2 cut(s) 458, 617
SmoI CTYRAG 2 cut(s) 458, 617
SphI GCATGC 2 cut(s) 436, 932
Sse9I AATT 5 cut(s) 274, 287, 421, 611, 963
SsiI CCGC 5 cut(s) 9, 12, 15, 30, 1075
SspMI CTAG 4 cut(s) 407, 1113, 1403, 1507
StyD4I CCNGG 6 cut(s) 592, 643, 778, 786, 1141, 1425
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 1 cut(s) 1363
TaiI ACGT 3 cut(s) 78, 559, 1168
TaqI TCGA 8 cut(s) 177, 244, 419, 599, 635, 682, 1135, 1432
TasI AATT 5 cut(s) 274, 287, 421, 611, 963
TatI WGTACW 2 cut(s) 571, 1017
TauI GCSGC 3 cut(s) 12, 15, 18
TfiI GAWTC 4 cut(s) 136, 270, 403, 1036
Tru1I TTAA 3 cut(s) 479, 996, 1149
Tru9I TTAA 3 cut(s) 479, 996, 1149
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 751, 1166
TseI GCWGC 6 cut(s) 260, 436, 587, 783, 1120, 1410
Tsp45I GTSAC 2 cut(s) 751, 1166
TspDTI ATGAA 9 cut(s) 135, 195, 220, 458, 941, 1049, 1065, 1386, 1445
TspGWI ACGGA 2 cut(s) 47, 1456
TspRI CASTG 1 cut(s) 886
Van91I CCANNNNNTGG 1 cut(s) 1198
VneI GTGCAC 1 cut(s) 733
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 2 cut(s) 421, 963
XceI RCATGY 3 cut(s) 436, 932, 1240
XmiI GTMKAC 1 cut(s) 797
XmnI GAANNNNTTC 3 cut(s) 240, 274, 605
XspI CTAG 4 cut(s) 407, 1113, 1403, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.