pycom01g06360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015716
Physical Location & Seq
Reverse (-)
6589206 .. 6589520
315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06360.1

Sequence Viewer

Length: 315 bp
ATGGCTTGGATCTTGCAAGTCCCCAACCGAGGAGCTTCCCTCACTCGGGAACTTAGGGGAGCACTGTTTGTACCATACTTGACCAATCCCGAAACTACTAAGCACCGGTCAACAGCATACCGTCAAGGACCCACAAGACTTTTCCTCCAACCAGGAGGCCAATCACAACACGACACATGTCGGTATCAGAAGCCAATCACAGCGCGACACGTGTCGATATCAGAGGCTAATCACAACACGACATGTGTCAATGTCAGAACAAAGCTAGAAACTCTCTTCTATAAAAGGGGATCATTCTCCCAAAATAATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

11.86

Weight (kDa)

10.42

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 205
AclWI GGATC 2 cut(s) 17, 298
AcvI CACGTG 1 cut(s) 211
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 3 cut(s) 29, 45, 46
AflIII ACRYGT 4 cut(s) 176, 208, 210, 242
AgeI ACCGGT 1 cut(s) 105
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 2 cut(s) 35, 265
AluI AGCT 2 cut(s) 35, 265
Alw21I GWGCWC 1 cut(s) 64
AlwI GGATC 2 cut(s) 17, 298
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 1 cut(s) 157
AsiGI ACCGGT 1 cut(s) 105
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 1 cut(s) 128
BbrPI CACGTG 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 153
BfaI CTAG 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 153
BoxI GACNNNNGTC 3 cut(s) 177, 211, 245
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BsaAI YACGTR 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 45, 46
Bse118I RCCGGY 1 cut(s) 105
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 28
BseLI CCNNNNNNNGG 3 cut(s) 29, 45, 46
BseRI GAGGAG 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 159
BshTI ACCGGT 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 3 cut(s) 29, 45, 46
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 2 cut(s) 9, 290
BspANI GGCC 1 cut(s) 159
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 2 cut(s) 17, 298
BsrFI RCCGGY 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 2 cut(s) 9, 290
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 66, 122
Bst6I CTCTTC 1 cut(s) 281
BstBAI YACGTR 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 53, 99
BstFNI CGCG 1 cut(s) 205
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 2 cut(s) 12, 293
BstMBI GATC 2 cut(s) 9, 290
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 180, 246
BstPAI GACNNNNGTC 3 cut(s) 177, 211, 245
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 1 cut(s) 205
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 71
CspAI ACCGGT 1 cut(s) 105
CviAII CATG 2 cut(s) 177, 243
CviJI RGCY 6 cut(s) 5, 35, 159, 193, 227, 265
CviKI_1 RGCY 6 cut(s) 5, 35, 159, 193, 227, 265
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 2 cut(s) 53, 99
DpnI GATC 2 cut(s) 11, 292
DpnII GATC 2 cut(s) 9, 290
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 128
Eco72I CACGTG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 151
EcoRV GATATC 1 cut(s) 219
FaeI CATG 2 cut(s) 180, 246
FaiI YATR 5 cut(s) 76, 118, 178, 244, 282
FaqI GGGAC 1 cut(s) 5
FatI CATG 2 cut(s) 176, 242
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 204
HaeIII GGCC 1 cut(s) 159
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 205
Hin1II CATG 2 cut(s) 180, 246
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HpaII CCGG 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 3 cut(s) 189, 223, 257
Hpy188III TCNNGA 2 cut(s) 47, 89
Hpy8I GTNNAC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 66, 122
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 1 cut(s) 16
HpyF3I CTNAG 2 cut(s) 53, 99
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 2 cut(s) 180, 246
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 2 cut(s) 9, 290
LmnI GCTCC 2 cut(s) 32, 59
LpnPI CCDG 3 cut(s) 119, 138, 165
MaeI CTAG 1 cut(s) 266
MaeII ACGT 1 cut(s) 210
MalI GATC 2 cut(s) 11, 292
MboI GATC 2 cut(s) 9, 290
MboII GAAGA 1 cut(s) 268
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 5 cut(s) 23, 50, 149, 155, 217
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 205
NdeII GATC 2 cut(s) 9, 290
NlaIII CATG 2 cut(s) 180, 246
NlaIV GGNNCC 1 cut(s) 130
NspI RCATGY 2 cut(s) 180, 246
PciI ACATGT 2 cut(s) 176, 242
PinAI ACCGGT 1 cut(s) 105
PmaCI CACGTG 1 cut(s) 211
PmlI CACGTG 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 2 cut(s) 176, 242
PshAI GACNNNNGTC 3 cut(s) 177, 211, 245
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 151
PspCI CACGTG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
Sau3AI GATC 2 cut(s) 9, 290
Sau96I GGNCC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 64
SetI ASST 3 cut(s) 37, 213, 267
SinI GGWCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 66, 122
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 215
TscAI CASTG 1 cut(s) 69
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 128
XceI RCATGY 2 cut(s) 180, 246
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.