pycom1146g00070

Mini-chromosome maintenance complex-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001146
OG0015716
Physical Location & Seq
Reverse (-)
123643 .. 124426
784 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1146g00070.3

Sequence Viewer

Length: 438 bp
ATGAGAAGCTTGGCACTCAATATGCTTAGCGTTTTCTACATTTCCCCCCTTCAAATTCAGTACAATTCCAATGACATTTTTCTTCTTCAAATTCTGTTGCAGCTTTTGATGCTAAAACCAAAGAGGGCAGAGAGACTGGACCACGTACTAACCTTGGGAACCAGCCAAGAAGAAGCAAGTAATTTCTATGATACCAGATCTCCTCGCTTAAAGACTTGGGGAACTCCTACTATATGGTTTGTATCGCACTTGACCAAACCCGAAACTACAAGTAAGCTTCAACTGAAAATGATACATTACCTTGTGCGTCAACGCCAACCAAAGATACCACTTCTGGATGAAGGAAAAGTACTTCCAAGAGAAGATGCCACATCTACCTACAGGACAGAGAAGGCAAGTGAAGGCGACACCACACTTCGGTACTTGGAAGTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MCM_bind

Protein Analysis

146

Amino Acids

16.87

Weight (kDa)

9.39

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 54, 90
AfaI GTAC 4 cut(s) 62, 147, 351, 422
AfiI CCNNNNNNNGG 1 cut(s) 417
AgsI TTSAA 3 cut(s) 53, 89, 281
AluBI AGCT 3 cut(s) 9, 103, 277
AluI AGCT 3 cut(s) 9, 103, 277
Alw26I GTCTC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 2 cut(s) 54, 90
AspS9I GGNCC 1 cut(s) 139
AvaII GGWCC 1 cut(s) 139
BarI GAAGNNNNNNTAC 2 cut(s) 333, 365
BbvI GCAGC 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 127
BfmI CTRYAG 1 cut(s) 379
BglII AGATCT 1 cut(s) 197
BisI GCNGC 1 cut(s) 101
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 102
BmcAI AGTACT 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 160
BmsI GCATC 2 cut(s) 99, 355
Bpu1102I GCTNAGC 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 141
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 197
Bsp1720I GCTNAGC 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 160
BsrI ACTGG 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 145
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 1 cut(s) 200
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 1 cut(s) 197
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 379
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BtsCI GGATG 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 139
CseI GACGC 1 cut(s) 296
Csp6I GTAC 4 cut(s) 61, 146, 350, 421
CviJI RGCY 4 cut(s) 9, 103, 165, 277
CviKI_1 RGCY 4 cut(s) 9, 103, 165, 277
CviQI GTAC 4 cut(s) 61, 146, 350, 421
DdeI CTNAG 1 cut(s) 26
DpnI GATC 1 cut(s) 199
DpnII GATC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaiI YATR 4 cut(s) 23, 189, 233, 235
Fnu4HI GCNGC 1 cut(s) 101
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 101
HgaI GACGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 311
HindII GTYRAC 1 cut(s) 311
HindIII AAGCTT 2 cut(s) 7, 275
Hpy166II GTNNAC 1 cut(s) 311
Hpy188III TCNNGA 2 cut(s) 335, 435
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 59, 335, 385, 395
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 144
Kzo9I GATC 1 cut(s) 197
LpnPI CCDG 5 cut(s) 122, 175, 208, 320, 367
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 2 cut(s) 99, 355
MaeII ACGT 1 cut(s) 144
MalI GATC 1 cut(s) 199
MboI GATC 1 cut(s) 197
MboII GAAGA 4 cut(s) 74, 77, 182, 374
MflI RGATCY 1 cut(s) 197
MluCI AATT 4 cut(s) 54, 64, 90, 181
MnlI CCTC 2 cut(s) 117, 213
MseI TTAA 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 109
NdeII GATC 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 160
PkrI GCNGC 1 cut(s) 102
Ppu21I YACGTR 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 139
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 4 cut(s) 62, 147, 351, 422
RsaNI GTAC 4 cut(s) 61, 146, 350, 421
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 1 cut(s) 197
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 1 cut(s) 351
SetI ASST 7 cut(s) 11, 105, 147, 155, 279, 303, 380
SfaNI GCATC 2 cut(s) 99, 355
SfcI CTRYAG 1 cut(s) 379
SinI GGWCC 1 cut(s) 139
Sse9I AATT 4 cut(s) 54, 64, 90, 181
StyI CCWWGG 1 cut(s) 153
TaiI ACGT 1 cut(s) 147
TasI AATT 4 cut(s) 54, 64, 90, 181
TatI WGTACW 2 cut(s) 60, 349
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 2 cut(s) 54, 90
ZrmI AGTACT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.