pycom520g01180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0015716
Physical Location & Seq
Forward (+)
892579 .. 894059
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g01180.7

Sequence Viewer

Length: 1131 bp
ATGAAATACTATTTATGGCCGCAAATATCATTAAGGCATCCTATAATAGTACGCATACATGTGCATCCCATAGGATTTAGAATAAATTTCGTCAATCCAAAATTCTTTATTAAATATATATTTGGAAACCAAATAAATATAAAATATTTAAAAGAAAAGACCCACTATCAATTCATTGTTAGGTCGAACCAACCTTGTGGGCATCGAAGATCCTTGCGATGCGTTTCACCAAAGTGGGGCCGAGTTATGGTCATTGTTGGCCAGAAAAATGGGTTGAAAGTGCTCCCCCTCGTGGCTTCTGTCCCCTCTAGTCAGAAAGAAAAAAATCTCTGCGAGGTTTCGTGCCTGGGTTCCAGTTTCGTGAGTCCACGCTTGTTTCTGGTTTCAAGGCCAAGAGACGAGGTGATGTTTAGGGTTTGGGGCGATGACACTCGGCGAAATGGTGAGTTGGTGGTGGCACAAGGGATGGAAAGTTTTGTACCATACTCGACCAATCCCGAAACTACTAAGCACCGGTCAACAGCATACCGTCAAGGACCCACAAGACTTTTCCTCCAACCAGGAGGCCAATCACAACATGACACATGTCAGTATCAAAGGCCAATCATAGCGCAACACGTGTCGATATTAGAGGCCAATCACAACACGACACGTGGGATCATTCTCCCACAAAAATCCCTAAGGTCATTTGTACCAAACTATTCACTAGAACTCACTCAAAGAGAGCTTGAACCTATGTATTTCTGGGTGAACCACGTACATCTTGTGTTATTTGCTTCCTTGTCTCTATTCATTTACATACTTATCCACACTAATGACCAGAGCAACCTAGTGAAGGTCACAAACTTCACACTTTCTGTTGTACCAAAGTCCTCGCCGATTTTGTGCATCAACAGAGAGTTTAAGAGAGAGAGAGTGAAAATGAGAGTGTTGAGGAAAAAAGAGATCATAGAAGACACGAGAGAGATTTCAAACTGGGGAGTCCCTTGTGGCCCTCGGTATTCGGTGTTTCGTAAAATCTTCATCGTAGCATTGAATTCAGTCCCGTTTGCATCCACGCGTTCGTATCGTCAAGTACTTCAAGCATATGTGTGTGTGTGTGTCTTTGGGTCAAACACAAATATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

377

Amino Acids

43.58

Weight (kDa)

10.12

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1060
AciI CCGC 1 cut(s) 20
AclWI GGATC 2 cut(s) 204, 665
AcoI YGGCCR 2 cut(s) 17, 259
AcsI RAATTY 3 cut(s) 85, 101, 1036
AcvI CACGTG 2 cut(s) 619, 653
AfaI GTAC 6 cut(s) 51, 480, 693, 759, 864, 1077
AfiI CCNNNNNNNGG 4 cut(s) 236, 247, 292, 835
AflIII ACRYGT 6 cut(s) 58, 584, 616, 618, 650, 1058
AgeI ACCGGT 1 cut(s) 513
AgsI TTSAA 6 cut(s) 277, 387, 731, 972, 1036, 1082
AjnI CCWGG 2 cut(s) 345, 559
AleI CACNNNNGTG 1 cut(s) 232
AluBI AGCT 1 cut(s) 727
AluI AGCT 1 cut(s) 727
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 390, 789
AlwI GGATC 2 cut(s) 204, 665
AoxI GGCC 8 cut(s) 17, 238, 259, 389, 565, 599, 633, 991
ApoI RAATTY 3 cut(s) 85, 101, 1036
AseI ATTAAT 1 cut(s) 1125
AsiGI ACCGGT 1 cut(s) 513
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 3 cut(s) 238, 536, 992
AsuHPI GGTGA 4 cut(s) 219, 415, 455, 760
AvaII GGWCC 1 cut(s) 536
AxyI CCTNAGG 1 cut(s) 680
BalI TGGCCA 1 cut(s) 261
BauI CACGAG 2 cut(s) 290, 958
BbrPI CACGTG 2 cut(s) 619, 653
BbsI GAAGAC 1 cut(s) 960
Bbv12I GWGCWC 1 cut(s) 285
BccI CCATC 1 cut(s) 460
BciT130I CCWGG 2 cut(s) 347, 561
BcoDI GTCTC 2 cut(s) 390, 789
BfaI CTAG 3 cut(s) 309, 707, 830
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
BmcAI AGTACT 1 cut(s) 1077
Bme1390I CCNGG 2 cut(s) 347, 561
Bme18I GGWCC 1 cut(s) 536
BmgT120I GGNCC 3 cut(s) 238, 536, 992
BmiI GGNNCC 3 cut(s) 239, 352, 538
BmrFI CCNGG 2 cut(s) 347, 561
BmrI ACTGGG 1 cut(s) 985
BmsI GCATC 6 cut(s) 46, 73, 209, 211, 897, 1061
BmuI ACTGGG 1 cut(s) 985
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 960
BsaAI YACGTR 3 cut(s) 619, 653, 757
BsaJI CCNNGG 2 cut(s) 346, 995
BsaWI WCCGGW 1 cut(s) 513
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 247, 292, 835
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 2 cut(s) 354, 980
Bse21I CCTNAGG 1 cut(s) 680
BseBI CCWGG 2 cut(s) 347, 561
BseDI CCNNGG 2 cut(s) 346, 995
BseGI GGATG 4 cut(s) 37, 64, 471, 1052
BseLI CCNNNNNNNGG 4 cut(s) 236, 247, 292, 835
BseNI ACTGG 2 cut(s) 354, 980
Bsh1236I CGCG 1 cut(s) 1060
BshFI GGCC 8 cut(s) 19, 240, 261, 391, 567, 601, 635, 993
BshTI ACCGGT 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 3 cut(s) 287, 968, 1028
BslI CCNNNNNNNGG 4 cut(s) 236, 247, 292, 835
BsmAI GTCTC 2 cut(s) 390, 789
BsmBI CGTCTC 1 cut(s) 390
BsmFI GGGAC 3 cut(s) 287, 968, 1028
BsnI GGCC 8 cut(s) 19, 240, 261, 391, 567, 601, 635, 993
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 3 cut(s) 209, 657, 945
BspACI CCGC 1 cut(s) 20
BspANI GGCC 8 cut(s) 19, 240, 261, 391, 567, 601, 635, 993
BspFNI CGCG 1 cut(s) 1060
BspLI GGNNCC 3 cut(s) 239, 352, 538
BspPI GGATC 2 cut(s) 204, 665
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 2 cut(s) 354, 980
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 346, 995
BssMI GATC 3 cut(s) 209, 657, 945
BssSI CACGAG 2 cut(s) 290, 958
Bst2BI CACGAG 2 cut(s) 290, 958
Bst2UI CCWGG 2 cut(s) 347, 561
Bst4CI ACNGT 1 cut(s) 530
BstBAI YACGTR 3 cut(s) 619, 653, 757
BstDEI CTNAG 2 cut(s) 507, 680
BstENI CCTNNNNNAGG 1 cut(s) 833
BstF5I GGATG 4 cut(s) 37, 64, 471, 1052
BstFNI CGCG 1 cut(s) 1060
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 3 cut(s) 212, 660, 948
BstMAI GTCTC 2 cut(s) 390, 789
BstMBI GATC 3 cut(s) 209, 657, 945
BstNI CCWGG 2 cut(s) 347, 561
BstNSI RCATGY 2 cut(s) 62, 588
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 345, 559
BstUI CGCG 1 cut(s) 1060
BstV2I GAAGAC 1 cut(s) 960
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 2 cut(s) 197, 269
BstYI RGATCY 1 cut(s) 209
Bsu36I CCTNAGG 1 cut(s) 680
BsuRI GGCC 8 cut(s) 19, 240, 261, 391, 567, 601, 635, 993
BtgZI GCGATG 2 cut(s) 232, 438
BtsCI GGATG 4 cut(s) 37, 64, 471, 1052
CfoI GCGC 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 238, 536, 992
Csp6I GTAC 6 cut(s) 50, 479, 692, 758, 863, 1076
CspAI ACCGGT 1 cut(s) 513
CviAII CATG 3 cut(s) 59, 578, 585
CviQI GTAC 6 cut(s) 50, 479, 692, 758, 863, 1076
DdeI CTNAG 2 cut(s) 507, 680
DpnI GATC 3 cut(s) 211, 659, 947
DpnII GATC 3 cut(s) 209, 657, 945
DraI TTTAAA 1 cut(s) 150
EaeI YGGCCR 2 cut(s) 17, 259
Eco47I GGWCC 1 cut(s) 536
Eco72I CACGTG 2 cut(s) 619, 653
Eco81I CCTNAGG 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 2 cut(s) 345, 559
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 3 cut(s) 62, 581, 588
FaqI GGGAC 3 cut(s) 287, 968, 1028
FatI CATG 3 cut(s) 58, 577, 584
FauNDI CATATG 1 cut(s) 1087
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 4 cut(s) 24, 51, 478, 1039
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 3 cut(s) 309, 707, 830
GlaI GCGC 1 cut(s) 612
GluI GCNGC 1 cut(s) 20
HaeIII GGCC 8 cut(s) 19, 240, 261, 391, 567, 601, 635, 993
HapII CCGG 1 cut(s) 514
HhaI GCGC 1 cut(s) 613
Hin1II CATG 3 cut(s) 62, 581, 588
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HinfI GANTC 2 cut(s) 364, 981
HpaII CCGG 1 cut(s) 514
HphI GGTGA 4 cut(s) 219, 415, 455, 760
Hpy166II GTNNAC 3 cut(s) 368, 519, 751
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 2 cut(s) 361, 497
Hpy8I GTNNAC 3 cut(s) 368, 519, 751
HpyAV CCTTC 1 cut(s) 829
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 3 cut(s) 618, 652, 756
HpyCH4V TGCA 3 cut(s) 64, 888, 1052
HpyF3I CTNAG 2 cut(s) 507, 680
HpySE526I ACGT 3 cut(s) 618, 652, 756
Hsp92II CATG 3 cut(s) 62, 581, 588
HspAI GCGC 1 cut(s) 611
Kzo9I GATC 3 cut(s) 209, 657, 945
LmnI GCTCC 1 cut(s) 288
LweI GCATC 6 cut(s) 46, 73, 209, 211, 897, 1061
MaeI CTAG 3 cut(s) 309, 707, 830
MaeII ACGT 3 cut(s) 618, 652, 756
MaeIII GTNAC 1 cut(s) 838
MalI GATC 3 cut(s) 211, 659, 947
MboI GATC 3 cut(s) 209, 657, 945
MboII GAAGA 3 cut(s) 219, 965, 1012
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 285
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 5 cut(s) 85, 101, 170, 1036, 1126
MluI ACGCGT 1 cut(s) 1058
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 2 cut(s) 373, 990
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 6 cut(s) 32, 111, 149, 903, 1125, 1129
MslI CAYNNNNRTG 4 cut(s) 59, 232, 813, 1090
Msp20I TGGCCA 1 cut(s) 261
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 2 cut(s) 347, 561
MvaI CCWGG 2 cut(s) 347, 561
MvnI CGCG 1 cut(s) 1060
NdeI CATATG 1 cut(s) 1087
NdeII GATC 3 cut(s) 209, 657, 945
NlaIII CATG 3 cut(s) 62, 581, 588
NlaIV GGNNCC 3 cut(s) 239, 352, 538
NmeAIII GCCGAG 2 cut(s) 266, 412
NmuCI GTSAC 1 cut(s) 838
NspI RCATGY 2 cut(s) 62, 588
OliI CACNNNNGTG 1 cut(s) 232
PacI TTAATTAA 1 cut(s) 1129
PciI ACATGT 2 cut(s) 58, 584
PinAI ACCGGT 1 cut(s) 513
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 2 cut(s) 372, 989
PmaCI CACGTG 2 cut(s) 619, 653
PmlI CACGTG 2 cut(s) 619, 653
PpsI GAGTC 2 cut(s) 372, 989
Ppu21I YACGTR 3 cut(s) 619, 653, 757
PpuMI RGGWCCY 1 cut(s) 536
PscI ACATGT 2 cut(s) 58, 584
PshAI GACNNNNGTC 1 cut(s) 585
PshBI ATTAAT 1 cut(s) 1125
Psp5II RGGWCCY 1 cut(s) 536
Psp6I CCWGG 2 cut(s) 345, 559
PspCI CACGTG 2 cut(s) 619, 653
PspGI CCWGG 2 cut(s) 345, 559
PspN4I GGNNCC 3 cut(s) 239, 352, 538
PspPI GGNCC 3 cut(s) 238, 536, 992
PspPPI RGGWCCY 1 cut(s) 536
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 6 cut(s) 51, 480, 693, 759, 864, 1077
RsaNI GTAC 6 cut(s) 50, 479, 692, 758, 863, 1076
RseI CAYNNNNRTG 4 cut(s) 59, 232, 813, 1090
SaqAI TTAA 6 cut(s) 32, 111, 149, 903, 1125, 1129
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 3 cut(s) 209, 657, 945
Sau96I GGNCC 3 cut(s) 238, 536, 992
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 2 cut(s) 373, 990
ScrFI CCNGG 2 cut(s) 347, 561
SduI GDGCHC 1 cut(s) 285
SfaNI GCATC 6 cut(s) 46, 73, 209, 211, 897, 1061
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 4 cut(s) 59, 232, 813, 1090
Sse9I AATT 5 cut(s) 85, 101, 170, 1036, 1126
SsiI CCGC 1 cut(s) 20
SspI AATATT 2 cut(s) 146, 1123
SspMI CTAG 3 cut(s) 309, 707, 830
StyD4I CCNGG 2 cut(s) 345, 559
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 3 cut(s) 621, 655, 759
TaqI TCGA 4 cut(s) 185, 205, 488, 623
TasI AATT 5 cut(s) 85, 101, 170, 1036, 1126
TatI WGTACW 1 cut(s) 1075
TauI GCSGC 1 cut(s) 22
Tru1I TTAA 6 cut(s) 32, 111, 149, 903, 1125, 1129
Tru9I TTAA 6 cut(s) 32, 111, 149, 903, 1125, 1129
TseFI GTSAC 1 cut(s) 838
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 4 cut(s) 17, 163, 781, 1012
VpaK11BI GGWCC 1 cut(s) 536
VspI ATTAAT 1 cut(s) 1125
XagI CCTNNNNNAGG 1 cut(s) 833
XapI RAATTY 3 cut(s) 85, 101, 1036
XceI RCATGY 2 cut(s) 62, 588
XspI CTAG 3 cut(s) 309, 707, 830
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.