pycom15g08640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015716
Physical Location & Seq
Reverse (-)
5496632 .. 5496946
315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08640.1

Sequence Viewer

Length: 315 bp
ATGGCTTGGATCTTGCAAGTCCCCAACCGAAGAGCTTCCCTCACTCAGGAACTTAGGGGAGCACTGTTTGTACCATACTTGACCAATCCCGAAACTACTAAGCACCGGTCAACAGCATACCGTCAAGGACCCACAAGACTTTTCCTCCAACCAGGAGGCCAATCACAACACGACACATGTCGGTATCAGAAGCCAATCACAGCACAACACGTGTCGATATCAGAGGCCAATCACAACATGACACGTGTCAATGTCAGAACAAAGCTAGAAACTCTCTTCTATAAAAGGGGATCATTCTCCCACAATAATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

11.99

Weight (kDa)

10.67

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 17, 298
AcvI CACGTG 2 cut(s) 211, 245
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 5 cut(s) 176, 208, 210, 242, 244
AgeI ACCGGT 1 cut(s) 105
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 2 cut(s) 35, 265
AluI AGCT 2 cut(s) 35, 265
Alw21I GWGCWC 1 cut(s) 64
AlwI GGATC 2 cut(s) 17, 298
AoxI GGCC 2 cut(s) 157, 225
AsiGI ACCGGT 1 cut(s) 105
Asp700I GAANNNNTTC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 128
AvaII GGWCC 1 cut(s) 128
BbrPI CACGTG 2 cut(s) 211, 245
Bbv12I GWGCWC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 153
BfaI CTAG 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 153
BoxI GACNNNNGTC 2 cut(s) 177, 245
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BsaAI YACGTR 2 cut(s) 211, 245
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 105
BseBI CCWGG 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 59
BshFI GGCC 2 cut(s) 159, 227
BshTI ACCGGT 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 2 cut(s) 159, 227
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 2 cut(s) 9, 290
BspANI GGCC 2 cut(s) 159, 227
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 2 cut(s) 17, 298
BspQI GCTCTTC 1 cut(s) 25
BsrFI RCCGGY 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 105
BssMI GATC 2 cut(s) 9, 290
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 66, 122
Bst6I CTCTTC 2 cut(s) 25, 281
BstBAI YACGTR 2 cut(s) 211, 245
BstDEI CTNAG 3 cut(s) 45, 53, 99
BstENI CCTNNNNNAGG 1 cut(s) 44
BstKTI GATC 2 cut(s) 12, 293
BstMBI GATC 2 cut(s) 9, 290
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 180
BstPAI GACNNNNGTC 2 cut(s) 177, 245
BstSCI CCNGG 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 2 cut(s) 159, 227
BtsIMutI CAGTG 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 71
CspAI ACCGGT 1 cut(s) 105
CviAII CATG 2 cut(s) 177, 238
CviJI RGCY 6 cut(s) 5, 35, 159, 193, 227, 265
CviKI_1 RGCY 6 cut(s) 5, 35, 159, 193, 227, 265
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 3 cut(s) 45, 53, 99
DpnI GATC 2 cut(s) 11, 292
DpnII GATC 2 cut(s) 9, 290
Eam1104I CTCTTC 2 cut(s) 25, 281
EarI CTCTTC 2 cut(s) 25, 281
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 128
Eco72I CACGTG 2 cut(s) 211, 245
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 151
EcoRV GATATC 1 cut(s) 219
FaeI CATG 2 cut(s) 180, 241
FaiI YATR 5 cut(s) 76, 118, 178, 239, 282
FaqI GGGAC 1 cut(s) 5
FatI CATG 2 cut(s) 176, 237
FspBI CTAG 1 cut(s) 266
HaeIII GGCC 2 cut(s) 159, 227
HapII CCGG 1 cut(s) 106
Hin1II CATG 2 cut(s) 180, 241
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HpaII CCGG 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 3 cut(s) 189, 223, 257
Hpy188III TCNNGA 2 cut(s) 47, 89
Hpy8I GTNNAC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 66, 122
HpyCH4IV ACGT 2 cut(s) 210, 244
HpyCH4V TGCA 1 cut(s) 16
HpyF3I CTNAG 3 cut(s) 45, 53, 99
HpySE526I ACGT 2 cut(s) 210, 244
Hsp92II CATG 2 cut(s) 180, 241
Kzo9I GATC 2 cut(s) 9, 290
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 4 cut(s) 32, 119, 138, 165
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 210, 244
MalI GATC 2 cut(s) 11, 292
MboI GATC 2 cut(s) 9, 290
MboII GAAGA 2 cut(s) 42, 268
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 4 cut(s) 50, 149, 155, 217
MroXI GAANNNNTTC 1 cut(s) 34
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
NdeII GATC 2 cut(s) 9, 290
NlaIII CATG 2 cut(s) 180, 241
NlaIV GGNNCC 1 cut(s) 130
NspI RCATGY 1 cut(s) 180
PciI ACATGT 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 105
PmaCI CACGTG 2 cut(s) 211, 245
PmlI CACGTG 2 cut(s) 211, 245
Ppu21I YACGTR 2 cut(s) 211, 245
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 1 cut(s) 176
PshAI GACNNNNGTC 2 cut(s) 177, 245
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 151
PspCI CACGTG 2 cut(s) 211, 245
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 25
Sau3AI GATC 2 cut(s) 9, 290
Sau96I GGNCC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 64
SetI ASST 4 cut(s) 37, 213, 247, 267
SinI GGWCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 66, 122
TaiI ACGT 2 cut(s) 213, 247
TaqI TCGA 1 cut(s) 215
TscAI CASTG 1 cut(s) 69
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 128
XagI CCTNNNNNAGG 1 cut(s) 44
XceI RCATGY 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.