pycom01g17900

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009588
Physical Location & Seq
Reverse (-)
17064590 .. 17066973
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1260 bp
ATGATAACCCTCTCTTCGCCTAAAACCCTATCTCCTTTCTCATTTCTCTGCAACCCCATCCCAAAACCCCAAATCCGAAACCCTAAATTCGCCGCGGTGACCCAATACTCAGACTCCAATTCGAAGCCAACCCTCGATTCCGATTGTGAATCGGTGTTCCAGACAGAGAGGGGTCTTAAATTCGACGTCGGCGGGACCTTCTTCCGCCACGAAAGCGCCACCGGCCGCGACCTCGGCGTGCTCGCCGCCGCTCTGTACAAGAAGTCCAAGGGCAAATTGCGAGTTCTCGATGCTCTGTGTGGCTGCGGGATTCGGTCTGTTCGGTACCTCGTCGAGGCGGAGGCCGATTTTGTTTTGGCAAATGACGCGAATGATGAGAACAAGAAAGTTATCACGGAGAACTTGTCGCAGGTGGAGAGTGGTTCCGGGGACGAGGCGAGGTGGGAGGTGTCGCTTACGGATGCGAATCGGATCATGACGGAGTGTTATCTGAAAAGGGATTTCTTTGATTTGATTGACATTGATTCGTTTGGGAGCGATTCGTCGTTTTTGAGGTCGGCGTTTAATGCTTTGAAGTTGAATGGATTGTTGTATGTTACTTCAACTGATGGGTTTTCGTCCGGTGGTCGCAGGCCTCAACAAACTTTAGCTGCATATGGAGCTTATGTCCGGCCAAAGCCATACTCGAATGAGCTCGGTTTGCGAATGCTTATTGGAGGGGCTGTGCGGGAAGCTTCAGTGCTGGGGTATCGAGTTACTCCACTGTTTTCATACTATTCATATCATGGACCAATTTTCAGAGTCATGCTCCGAGTCAACCGGGGAAAGCTTCCTGATAACAAACATTATGGGTTCATCAGCTACTGCAACGGCTGTGGAAGTTCTCAAGAGTACTCATGGGCTGAACTTGGTCGGATCGGTTGCCCCTGCAGTGACTCAAAGGTCTCCAAGTCACTTGTTGTTTCTGGCCCTCTCTGGACAGGGCCGTTGCATGACACTGCTTATATTACAGAAATGTTAAGTTTGGCCGAGGAATGGGGATGGGCAGGCATTGAAACAGGAACTGATCTGGACAAACTTTTGAACCAAATGGTGGCTGAGAGCGACCCCAGATTACCCTTTGGCTACACCAAGTTGGACGAGGTGGCAAGCCGTGCGAAAGTAAATTCTCCGTCACTGAGAGCCGTGATGTCCACCCTTCAAAAGGCATGGTGGACACTTGTTGGGTACGGTTTTGCATGTTTGCCCATTGTATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

420

Amino Acids

46.27

Weight (kDa)

7.47

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM PF02005 68 - 405 8.7e-56 N2,N2-dimethylguanosine tRNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
AasI GACNNNNNNGTC 1 cut(s) 941
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 400
Acc65I GGTACC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 324
AccB7I CCANNNNNTGG 2 cut(s) 1093, 1254
AccBSI CCGCTC 1 cut(s) 251
AccII CGCG 3 cut(s) 95, 228, 368
AclWI GGATC 2 cut(s) 479, 923
AcoI YGGCCR 3 cut(s) 223, 671, 1026
AcsI RAATTY 3 cut(s) 86, 179, 1165
AcuI CTGAAG 1 cut(s) 720
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 4 cut(s) 257, 326, 893, 1229
AfiI CCNNNNNNNGG 5 cut(s) 334, 1035, 1093, 1204, 1254
AgsI TTSAA 6 cut(s) 574, 580, 603, 1055, 1084, 1202
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AluBI AGCT 6 cut(s) 650, 662, 694, 734, 829, 861
AluI AGCT 6 cut(s) 650, 662, 694, 734, 829, 861
Alw21I GWGCWC 2 cut(s) 243, 696
Alw26I GTCTC 1 cut(s) 949
AlwI GGATC 2 cut(s) 479, 923
AlwNI CAGNNNCTG 2 cut(s) 864, 1064
AoxI GGCC 7 cut(s) 223, 342, 632, 671, 967, 983, 1026
ApeKI GCWGC 2 cut(s) 303, 650
ApoI RAATTY 3 cut(s) 86, 179, 1165
Asp718I GGTACC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 4 cut(s) 195, 788, 968, 983
AsuC2I CCSGG 2 cut(s) 427, 821
AsuHPI GGTGA 1 cut(s) 109
AsuII TTCGAA 1 cut(s) 122
AvaII GGWCC 2 cut(s) 195, 788
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 243, 696
BbvI GCAGC 2 cut(s) 290, 637
BccI CCATC 3 cut(s) 65, 602, 1035
BceAI ACGGC 4 cut(s) 886, 970, 1137, 1169
BcnI CCSGG 2 cut(s) 427, 821
BcoDI GTCTC 1 cut(s) 949
BfmI CTRYAG 1 cut(s) 928
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 400
BisI GCNGC 6 cut(s) 93, 226, 246, 249, 304, 651
BlsI GCNGC 6 cut(s) 94, 227, 247, 250, 305, 652
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 2 cut(s) 427, 821
Bme18I GGWCC 2 cut(s) 195, 788
BmgT120I GGNCC 4 cut(s) 195, 788, 968, 983
BmiI GGNNCC 3 cut(s) 196, 326, 424
BmrFI CCNGG 2 cut(s) 427, 821
BmsI GCATC 2 cut(s) 280, 451
BplI GAGNNNNNCTC 2 cut(s) 792, 824
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 870
BpuMI CCSGG 2 cut(s) 427, 821
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 949
BsaJI CCNNGG 6 cut(s) 93, 232, 267, 426, 820, 1029
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 1035, 1093, 1204, 1254
Bse118I RCCGGY 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 465
BseDI CCNNGG 6 cut(s) 93, 232, 267, 426, 820, 1029
BseGI GGATG 3 cut(s) 57, 466, 1046
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 5 cut(s) 334, 1035, 1093, 1204, 1254
BseMII CTCAG 3 cut(s) 123, 1089, 1169
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 2 cut(s) 290, 637
BseYI CCCAGC 1 cut(s) 742
Bsh1236I CGCG 3 cut(s) 95, 228, 368
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BshNI GGYRCC 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 243, 696
BsiSI CCGG 5 cut(s) 222, 426, 621, 670, 820
BslFI GGGAC 2 cut(s) 208, 443
BslI CCNNNNNNNGG 5 cut(s) 334, 1035, 1093, 1204, 1254
BsmAI GTCTC 1 cut(s) 949
BsmFI GGGAC 2 cut(s) 208, 443
BsmI GAATGC 1 cut(s) 711
BsnI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
Bso31I GGTCTC 1 cut(s) 949
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 243, 696
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 3 cut(s) 471, 915, 1066
BspANI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BspCNI CTCAG 3 cut(s) 122, 1090, 1170
BspFNI CGCG 3 cut(s) 95, 228, 368
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 196, 326, 424
BspMAI CTGCAG 1 cut(s) 932
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 2 cut(s) 479, 923
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 949
BsrBI CCGCTC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 6 cut(s) 93, 232, 267, 426, 820, 1029
BssMI GATC 3 cut(s) 471, 915, 1066
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 765, 1232
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 5 cut(s) 239, 243, 632, 1048, 1150
BstDEI CTNAG 3 cut(s) 109, 1098, 1178
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 97
BstENI CCTNNNNNAGG 2 cut(s) 332, 1202
BstF5I GGATG 3 cut(s) 57, 466, 1046
BstFNI CGCG 3 cut(s) 95, 228, 368
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 474, 918, 1069
BstMAI GTCTC 1 cut(s) 949
BstMBI GATC 3 cut(s) 471, 915, 1066
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 8 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154
BstNSI RCATGY 1 cut(s) 1242
BstPI GGTNACC 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 425, 819
BstSFI CTRYAG 1 cut(s) 928
BstUI CGCG 3 cut(s) 95, 228, 368
BstV1I GCAGC 2 cut(s) 290, 637
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 3 cut(s) 57, 466, 1046
BtsI GCAGTG 2 cut(s) 937, 996
BtsIMutI CAGTG 5 cut(s) 744, 761, 937, 996, 1175
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 5 cut(s) 239, 243, 632, 1048, 1150
CaiI CAGNNNCTG 2 cut(s) 864, 1064
CciI TCATGA 1 cut(s) 474
CfoI GCGC 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 4 cut(s) 195, 788, 968, 983
Cfr42I CCGCGG 1 cut(s) 96
CseI GACGC 1 cut(s) 374
Csp6I GTAC 4 cut(s) 256, 325, 892, 1228
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 7 cut(s) 475, 785, 805, 897, 992, 1209, 1239
CviQI GTAC 4 cut(s) 256, 325, 892, 1228
DdeI CTNAG 3 cut(s) 109, 1098, 1178
DpnI GATC 3 cut(s) 473, 917, 1068
DpnII GATC 3 cut(s) 471, 915, 1066
DrdI GACNNNNNNGTC 1 cut(s) 941
DseDI GACNNNNNNGTC 1 cut(s) 941
EaeI YGGCCR 3 cut(s) 223, 671, 1026
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
EciI GGCGGA 2 cut(s) 194, 353
Ecl136II GAGCTC 1 cut(s) 694
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 634
Eco24I GRGCYC 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 195, 788
Eco52I CGGCCG 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 694
Eco57I CTGAAG 1 cut(s) 720
Eco91I GGTNACC 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 694
EcoNI CCTNNNNNAGG 2 cut(s) 332, 1202
EcoO109I RGGNCCY 1 cut(s) 195
EcoO65I GGTNACC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 7 cut(s) 478, 788, 808, 900, 995, 1212, 1242
FaqI GGGAC 2 cut(s) 208, 443
FatI CATG 7 cut(s) 474, 784, 804, 896, 991, 1208, 1238
FauI CCCGC 3 cut(s) 185, 299, 720
FauNDI CATATG 1 cut(s) 655
Fnu4HI GCNGC 6 cut(s) 93, 226, 246, 249, 304, 651
FokI GGATG 3 cut(s) 44, 473, 1053
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 6 cut(s) 93, 226, 246, 249, 304, 651
GlaI GCGC 1 cut(s) 217
GluI GCNGC 6 cut(s) 93, 226, 246, 249, 304, 651
GsaI CCCAGC 1 cut(s) 746
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 7 cut(s) 225, 344, 634, 673, 969, 985, 1028
HapII CCGG 5 cut(s) 222, 426, 621, 670, 820
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 7 cut(s) 478, 788, 808, 900, 995, 1212, 1242
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 2 cut(s) 732, 827
HpaII CCGG 5 cut(s) 222, 426, 621, 670, 820
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 3 cut(s) 817, 1194, 1215
Hpy188I TCNGA 8 cut(s) 77, 112, 142, 471, 492, 800, 812, 915
Hpy188III TCNNGA 7 cut(s) 160, 287, 475, 833, 887, 976, 1070
Hpy8I GTNNAC 3 cut(s) 817, 1194, 1215
Hpy99I CGWCG 4 cut(s) 188, 191, 335, 547
HpyAV CCTTC 2 cut(s) 208, 1208
HpyCH4III ACNGT 2 cut(s) 765, 1232
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 6 cut(s) 51, 653, 867, 930, 991, 1238
HpyF10VI GCNNNNNNNGC 8 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154
HpyF3I CTNAG 3 cut(s) 109, 1098, 1178
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 7 cut(s) 478, 788, 808, 900, 995, 1212, 1242
HspAI GCGC 1 cut(s) 216
KpnI GGTACC 1 cut(s) 328
KspI CCGCGG 1 cut(s) 96
Kzo9I GATC 3 cut(s) 471, 915, 1066
LmnI GCTCC 3 cut(s) 534, 659, 813
Lsp1109I GCAGC 2 cut(s) 290, 637
LweI GCATC 2 cut(s) 280, 451
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 6 cut(s) 97, 595, 754, 932, 951, 1173
MalI GATC 3 cut(s) 473, 917, 1068
MbiI CCGCTC 1 cut(s) 251
MboI GATC 3 cut(s) 471, 915, 1066
MboII GAAGA 2 cut(s) 6, 193
MhlI GDGCHC 2 cut(s) 243, 696
MluCI AATT 6 cut(s) 86, 118, 179, 275, 792, 1165
MlyI GAGTC 4 cut(s) 107, 810, 822, 929
MmeI TCCRAC 2 cut(s) 893, 1116
MseI TTAA 3 cut(s) 177, 564, 1019
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 5 cut(s) 222, 426, 621, 670, 820
MspR9I CCNGG 2 cut(s) 427, 821
Mva1269I GAATGC 1 cut(s) 711
MvnI CGCG 3 cut(s) 95, 228, 368
MwoI GCNNNNNNNGC 8 cut(s) 213, 222, 234, 365, 566, 659, 700, 1154
NciI CCSGG 2 cut(s) 427, 821
NdeI CATATG 1 cut(s) 655
NdeII GATC 3 cut(s) 471, 915, 1066
NlaIII CATG 7 cut(s) 478, 788, 808, 900, 995, 1212, 1242
NlaIV GGNNCC 3 cut(s) 196, 326, 424
NmeAIII GCCGAG 2 cut(s) 213, 1054
NmuCI GTSAC 4 cut(s) 97, 932, 951, 1173
NspI RCATGY 1 cut(s) 1242
NspV TTCGAA 1 cut(s) 122
PagI TCATGA 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 400
PceI AGGCCT 1 cut(s) 634
PctI GAATGC 1 cut(s) 711
PfeI GAWTC 6 cut(s) 137, 149, 310, 466, 524, 539
PflMI CCANNNNNTGG 2 cut(s) 1093, 1254
PkrI GCNGC 6 cut(s) 94, 227, 247, 250, 305, 652
PleI GAGTC 4 cut(s) 107, 809, 821, 929
PpsI GAGTC 4 cut(s) 107, 809, 821, 929
PpuMI RGGWCCY 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 195
PspEI GGTNACC 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 742
PspN4I GGNNCC 3 cut(s) 196, 326, 424
PspPI GGNCC 4 cut(s) 195, 788, 968, 983
PspPPI RGGWCCY 1 cut(s) 195
PstI CTGCAG 1 cut(s) 932
PstNI CAGNNNCTG 2 cut(s) 864, 1064
RsaI GTAC 4 cut(s) 257, 326, 893, 1229
RsaNI GTAC 4 cut(s) 256, 325, 892, 1228
SacI GAGCTC 1 cut(s) 696
SacII CCGCGG 1 cut(s) 96
SaqAI TTAA 3 cut(s) 177, 564, 1019
SatI GCNGC 6 cut(s) 93, 226, 246, 249, 304, 651
Sau3AI GATC 3 cut(s) 471, 915, 1066
Sau96I GGNCC 4 cut(s) 195, 788, 968, 983
ScaI AGTACT 1 cut(s) 893
SchI GAGTC 4 cut(s) 107, 810, 822, 929
ScrFI CCNGG 2 cut(s) 427, 821
SduI GDGCHC 2 cut(s) 243, 696
SfaNI GCATC 2 cut(s) 280, 451
SfcI CTRYAG 1 cut(s) 928
Sfr303I CCGCGG 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 122
SgrBI CCGCGG 1 cut(s) 96
SinI GGWCC 2 cut(s) 195, 788
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 6 cut(s) 86, 118, 179, 275, 792, 1165
SseBI AGGCCT 1 cut(s) 634
SstI GAGCTC 1 cut(s) 696
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 2 cut(s) 425, 819
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 765, 1232
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 122, 135, 183, 288, 333, 686, 751
TaqII GACCGA 1 cut(s) 303
TasI AATT 6 cut(s) 86, 118, 179, 275, 792, 1165
TatI WGTACW 2 cut(s) 255, 891
TauI GCSGC 4 cut(s) 95, 228, 248, 251
TfiI GAWTC 6 cut(s) 137, 149, 310, 466, 524, 539
Tru1I TTAA 3 cut(s) 177, 564, 1019
Tru9I TTAA 3 cut(s) 177, 564, 1019
TscAI CASTG 5 cut(s) 744, 768, 937, 1003, 1182
TseFI GTSAC 4 cut(s) 97, 932, 951, 1173
TseI GCWGC 2 cut(s) 303, 650
Tsp45I GTSAC 4 cut(s) 97, 932, 951, 1173
TspDTI ATGAA 3 cut(s) 759, 768, 844
TspGWI ACGGA 4 cut(s) 410, 473, 494, 1161
TspRI CASTG 5 cut(s) 744, 768, 937, 1003, 1182
Van91I CCANNNNNTGG 2 cut(s) 1093, 1254
VpaK11BI GGWCC 2 cut(s) 195, 788
XagI CCTNNNNNAGG 2 cut(s) 332, 1202
XapI RAATTY 3 cut(s) 86, 179, 1165
XceI RCATGY 1 cut(s) 1242
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.