pycom01g21920

Glutaminyl-peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007377
Physical Location & Seq
Reverse (-)
19722338 .. 19725161
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1164 bp
ATGTCGTTTAATTTGAAAAATTATCTACAAATTTGGCCTAATAAATATTTCGGCGCGTGTAAGCTCAACGGTCAAGACAATGACACCAACCTCGGCGCGTGTTACCGCAAGCATGCCGTTTCCACCTGCAAACCACTCGAGCGCAGCCTTTTACCTCAAACCCCCTTTCTGATCCACCGGTTCATGGCCACCGGTTCGCTTAGGAAGAAGCTGAATAACAATAAGCGGTCCGACCGCAGGCCATACAGTAACGCGCCCATGGCGGCCTCTCGGCGGTCGCTTCCAAACTACAGGAAAACATCTCTGATCCTCGCCGCTTTTCTCATGCTCGGCGTTCTTATTCTCCTCGGCATTTCGTCGAACATGTGGACCACCGTCAGCTACGCCGACGTCTCCCCTGGAATTTACTCGATCGAGGTCGTCCATGAGTTCCCTCACGATCCCGACGCCTTCACCCAGGGGCTACTTTATGCTGGAAATGATACCTTGTTCGAGTCGACTGGGCTTTACGGGAAGTCATCAGTGCGCAAGGTTGCAATTCGAACCGGAAAGGTTGAAGCACTTCAGAAGATGGACCCTTCTTACTTTGGGGAGGGATTAACTCTCCTTGGTGAAAGGCTATTCCAAGTAACTTGGTTGAGGAAAATTGGTTTCATATATGACCGAAAAAATTTAACCAAATTTGAGAAGTTCACGCATCAGATGAAAGATGGCTGGGGACTGGCTACTGATGGAAAAGTGTTGTTTGGAAGTGATGGCAGTTCAGTGTTGTACAAGATTGACCCTCAAACATTAAAAGTTATGGACAGACGTATGGTCAAGTATAAAGATCAAGAAGTGCAAAACCTCAACGAGTTAGAGTTTATAAACGGCGAAGTTTGGGCAAATGTATATCAGACAGATTGCATAGCTAGAATCTCGCATCAAGATGGTAGGGTGCTTGGATGGATCGTCCTTCCACAATTAAGGGATGGCTTACTAGCTTCTGGGAATCATGGTATAGATGTTCTAAACGGCATTGCATGGGACAGTGACAAAAACCGTATATTTGTGACCGGAAAACTATGGCCAAAGCTTTACGAGATCAAGCTACATCCGATGAAGAAACAGTTCAAAGACGGGGCCATTGAGCGGCTTTGCATGCGGAGGCCATTCAATTTTTGA

Protein Analysis

388

Amino Acids

44.18

Weight (kDa)

9.6

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 129 - 364 7.1e-93 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AarI CACCTGC 1 cut(s) 134
AatII GACGTC 1 cut(s) 393
Acc16I TGCGCA 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 134
AccBSI CCGCTC 1 cut(s) 1132
AccI GTMKAC 1 cut(s) 497
AccII CGCG 3 cut(s) 56, 98, 254
AciI CCGC 8 cut(s) 106, 226, 235, 263, 274, 315, 1132, 1144
AclWI GGATC 4 cut(s) 166, 301, 434, 956
AcoI YGGCCR 2 cut(s) 186, 1067
AcsI RAATTY 4 cut(s) 30, 402, 670, 680
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 2 cut(s) 390, 447
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 4 cut(s) 184, 237, 273, 1131
AflIII ACRYGT 1 cut(s) 363
AgeI ACCGGT 2 cut(s) 177, 191
AgsI TTSAA 4 cut(s) 16, 557, 1114, 1156
AhdI GACNNNNNGTC 1 cut(s) 815
AjnI CCWGG 2 cut(s) 397, 456
AluBI AGCT 7 cut(s) 64, 211, 381, 911, 983, 1075, 1090
AluI AGCT 7 cut(s) 64, 211, 381, 911, 983, 1075, 1090
Alw26I GTCTC 1 cut(s) 397
AlwI GGATC 4 cut(s) 166, 301, 434, 956
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 7 cut(s) 35, 186, 239, 264, 1067, 1122, 1148
ApeKI GCWGC 1 cut(s) 144
ApoI RAATTY 4 cut(s) 30, 402, 670, 680
AsiGI ACCGGT 2 cut(s) 177, 191
Asp700I GAANNNNTTC 2 cut(s) 561, 1109
AspLEI GCGC 5 cut(s) 56, 98, 144, 256, 528
AspS9I GGNCC 4 cut(s) 228, 369, 574, 1122
AsuHPI GGTGA 2 cut(s) 445, 623
AsuII TTCGAA 1 cut(s) 541
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 3 cut(s) 228, 369, 574
BalI TGGCCA 2 cut(s) 188, 1069
BbvI GCAGC 1 cut(s) 156
BccI CCATC 7 cut(s) 565, 704, 725, 749, 923, 939, 965
BceAI ACGGC 3 cut(s) 101, 886, 1030
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 399, 458
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 2 cut(s) 912, 980
BfmI CTRYAG 1 cut(s) 289
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 4 cut(s) 145, 264, 315, 1133
BlsI GCNGC 4 cut(s) 146, 265, 316, 1134
Bme1390I CCNGG 2 cut(s) 399, 458
Bme18I GGWCC 3 cut(s) 228, 369, 574
BmeRI GACNNNNNGTC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 4 cut(s) 228, 369, 574, 1122
BmiI GGNNCC 2 cut(s) 576, 1123
BmrFI CCNGG 2 cut(s) 399, 458
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 2 cut(s) 706, 931
BmuI ACTGGG 1 cut(s) 510
BoxI GACNNNNGTC 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 541
BsaHI GRCGYC 2 cut(s) 390, 447
BsaJI CCNNGG 7 cut(s) 91, 258, 346, 397, 456, 457, 607
BsaWI WCCGGW 4 cut(s) 177, 191, 545, 1055
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 237, 273, 1131
Bse118I RCCGGY 2 cut(s) 177, 191
Bse1I ACTGG 2 cut(s) 505, 726
Bse3DI GCAATG 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 399, 458
BseDI CCNNGG 7 cut(s) 91, 258, 346, 397, 456, 457, 607
BseGI GGATG 3 cut(s) 950, 976, 1093
BseLI CCNNNNNNNGG 4 cut(s) 184, 237, 273, 1131
BseMI GCAATG 1 cut(s) 1017
BseNI ACTGG 2 cut(s) 505, 726
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 1 cut(s) 156
BseYI CCCAGC 1 cut(s) 714
Bsh1236I CGCG 3 cut(s) 56, 98, 254
Bsh1285I CGRYCG 3 cut(s) 235, 278, 414
BshFI GGCC 7 cut(s) 37, 188, 241, 266, 1069, 1124, 1150
BshTI ACCGGT 2 cut(s) 177, 191
BsiEI CGRYCG 3 cut(s) 235, 278, 414
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 4 cut(s) 178, 192, 546, 1056
BslFI GGGAC 2 cut(s) 732, 1040
BslI CCNNNNNNNGG 4 cut(s) 184, 237, 273, 1131
BsmAI GTCTC 1 cut(s) 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 2 cut(s) 732, 1040
BsnI GGCC 7 cut(s) 37, 188, 241, 266, 1069, 1124, 1150
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 8 cut(s) 106, 226, 235, 263, 274, 315, 1132, 1144
BspANI GGCC 7 cut(s) 37, 188, 241, 266, 1069, 1124, 1150
BspFNI CGCG 3 cut(s) 56, 98, 254
BspLI GGNNCC 2 cut(s) 576, 1123
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 4 cut(s) 166, 301, 434, 956
BspT104I TTCGAA 1 cut(s) 541
BsrBI CCGCTC 1 cut(s) 1132
BsrDI GCAATG 1 cut(s) 1017
BsrFI RCCGGY 2 cut(s) 177, 191
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 2 cut(s) 505, 726
BssAI RCCGGY 2 cut(s) 177, 191
BssECI CCNNGG 7 cut(s) 91, 258, 346, 397, 456, 457, 607
BssMI GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
BssNI GRCGYC 2 cut(s) 390, 447
BssT1I CCWWGG 2 cut(s) 258, 607
Bst2UI CCWGG 2 cut(s) 399, 458
Bst4CI ACNGT 6 cut(s) 71, 248, 376, 1031, 1043, 1110
BstACI GRCGYC 2 cut(s) 390, 447
BstAUI TGTACA 1 cut(s) 771
BstBI TTCGAA 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 110, 114, 239, 1142
BstDEI CTNAG 1 cut(s) 200
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 3 cut(s) 950, 976, 1093
BstFNI CGCG 3 cut(s) 56, 98, 254
BstHHI GCGC 5 cut(s) 56, 98, 144, 256, 528
BstKTI GATC 7 cut(s) 174, 309, 414, 442, 832, 951, 1086
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
BstMCI CGRYCG 3 cut(s) 235, 278, 414
BstMWI GCNNNNNNNGC 2 cut(s) 260, 1141
BstNI CCWGG 2 cut(s) 399, 458
BstNSI RCATGY 3 cut(s) 116, 367, 1144
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 397, 456
BstSFI CTRYAG 1 cut(s) 289
BstUI CGCG 3 cut(s) 56, 98, 254
BstV1I GCAGC 1 cut(s) 156
BsuRI GGCC 7 cut(s) 37, 188, 241, 266, 1069, 1124, 1150
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 950, 976, 1093
BtsIMutI CAGTG 3 cut(s) 528, 771, 1036
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 4 cut(s) 110, 114, 239, 1142
CfoI GCGC 5 cut(s) 56, 98, 144, 256, 528
Cfr10I RCCGGY 2 cut(s) 177, 191
Cfr13I GGNCC 4 cut(s) 228, 369, 574, 1122
CpoI CGGWCCG 1 cut(s) 228
CseI GACGC 1 cut(s) 455
Csp6I GTAC 1 cut(s) 772
CspAI ACCGGT 2 cut(s) 177, 191
CspI CGGWCCG 1 cut(s) 228
CviAII CATG 9 cut(s) 113, 184, 259, 325, 364, 425, 995, 1023, 1141
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 1 cut(s) 200
DpnI GATC 7 cut(s) 173, 308, 413, 441, 831, 950, 1085
DpnII GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
DriI GACNNNNNGTC 1 cut(s) 815
EaeI YGGCCR 2 cut(s) 186, 1067
Eam1105I GACNNNNNGTC 1 cut(s) 815
Eco130I CCWWGG 2 cut(s) 258, 607
Eco47I GGWCC 3 cut(s) 228, 369, 574
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 397, 456
EcoT14I CCWWGG 2 cut(s) 258, 607
ErhI CCWWGG 2 cut(s) 258, 607
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 9 cut(s) 116, 187, 262, 328, 367, 428, 998, 1026, 1144
FaqI GGGAC 2 cut(s) 732, 1040
FatI CATG 9 cut(s) 112, 183, 258, 324, 363, 424, 994, 1022, 1140
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 4 cut(s) 145, 264, 315, 1133
FokI GGATG 3 cut(s) 957, 983, 1080
Fsp4HI GCNGC 4 cut(s) 145, 264, 315, 1133
FspBI CTAG 2 cut(s) 912, 980
FspI TGCGCA 1 cut(s) 527
GlaI GCGC 5 cut(s) 55, 97, 143, 255, 527
GluI GCNGC 4 cut(s) 145, 264, 315, 1133
GsaI CCCAGC 1 cut(s) 718
HaeIII GGCC 7 cut(s) 37, 188, 241, 266, 1069, 1124, 1150
HapII CCGG 4 cut(s) 178, 192, 546, 1056
HgaI GACGC 1 cut(s) 455
HhaI GCGC 5 cut(s) 56, 98, 144, 256, 528
Hin1I GRCGYC 2 cut(s) 390, 447
Hin1II CATG 9 cut(s) 116, 187, 262, 328, 367, 428, 998, 1026, 1144
Hin6I GCGC 5 cut(s) 54, 96, 142, 254, 526
HinP1I GCGC 5 cut(s) 54, 96, 142, 254, 526
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 1073
HinfI GANTC 3 cut(s) 494, 915, 991
HpaII CCGG 4 cut(s) 178, 192, 546, 1056
HphI GGTGA 2 cut(s) 445, 623
Hpy166II GTNNAC 3 cut(s) 369, 498, 693
Hpy188I TCNGA 7 cut(s) 171, 232, 306, 567, 702, 897, 1098
Hpy188III TCNNGA 5 cut(s) 74, 437, 443, 833, 926
Hpy8I GTNNAC 3 cut(s) 369, 498, 693
Hpy99I CGWCG 3 cut(s) 361, 392, 449
HpyAV CCTTC 3 cut(s) 460, 588, 965
HpyCH4III ACNGT 6 cut(s) 71, 248, 376, 1031, 1043, 1110
HpyCH4IV ACGT 2 cut(s) 390, 811
HpyCH4V TGCA 6 cut(s) 129, 536, 841, 906, 1022, 1140
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 1141
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 2 cut(s) 390, 811
Hsp92I GRCGYC 2 cut(s) 390, 447
Hsp92II CATG 9 cut(s) 116, 187, 262, 328, 367, 428, 998, 1026, 1144
HspAI GCGC 5 cut(s) 54, 96, 142, 254, 526
Kzo9I GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
Lsp1109I GCAGC 1 cut(s) 156
LweI GCATC 2 cut(s) 706, 931
MaeI CTAG 2 cut(s) 912, 980
MaeII ACGT 2 cut(s) 390, 811
MaeIII GTNAC 5 cut(s) 101, 248, 628, 1031, 1051
MalI GATC 7 cut(s) 173, 308, 413, 441, 831, 950, 1085
MbiI CCGCTC 1 cut(s) 1132
MboI GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
MboII GAAGA 3 cut(s) 217, 580, 1114
MlsI TGGCCA 2 cut(s) 188, 1069
MluNI TGGCCA 2 cut(s) 188, 1069
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 255
Mox20I TGGCCA 2 cut(s) 188, 1069
MroXI GAANNNNTTC 2 cut(s) 561, 1109
MscI TGGCCA 2 cut(s) 188, 1069
MseI TTAA 5 cut(s) 9, 599, 674, 794, 965
MslI CAYNNNNRTG 1 cut(s) 927
Msp20I TGGCCA 2 cut(s) 188, 1069
MspI CCGG 4 cut(s) 178, 192, 546, 1056
MspR9I CCNGG 2 cut(s) 399, 458
MvaI CCWGG 2 cut(s) 399, 458
MvnI CGCG 3 cut(s) 56, 98, 254
MwoI GCNNNNNNNGC 2 cut(s) 260, 1141
NcoI CCATGG 1 cut(s) 258
NdeII GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
NlaIII CATG 9 cut(s) 116, 187, 262, 328, 367, 428, 998, 1026, 1144
NlaIV GGNNCC 2 cut(s) 576, 1123
NmeAIII GCCGAG 4 cut(s) 72, 250, 309, 327
NmuCI GTSAC 2 cut(s) 1031, 1051
NsbI TGCGCA 1 cut(s) 527
NspI RCATGY 3 cut(s) 116, 367, 1144
NspV TTCGAA 1 cut(s) 541
PaeI GCATGC 2 cut(s) 116, 1144
PaeR7I CTCGAG 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 134
PasI CCCWGGG 1 cut(s) 457
PciI ACATGT 1 cut(s) 363
PdmI GAANNNNTTC 2 cut(s) 561, 1109
PfeI GAWTC 2 cut(s) 915, 991
PinAI ACCGGT 2 cut(s) 177, 191
PkrI GCNGC 4 cut(s) 146, 265, 316, 1134
Ple19I CGATCG 1 cut(s) 414
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
PscI ACATGT 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 374
PsiI TTATAA 1 cut(s) 866
Psp6I CCWGG 2 cut(s) 397, 456
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 2 cut(s) 397, 456
PspN4I GGNNCC 2 cut(s) 576, 1123
PspPI GGNCC 4 cut(s) 228, 369, 574, 1122
PspXI VCTCGAGB 1 cut(s) 137
PvuI CGATCG 1 cut(s) 414
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 1 cut(s) 927
Rsr2I CGGWCCG 1 cut(s) 228
RsrII CGGWCCG 1 cut(s) 228
SalI GTCGAC 1 cut(s) 496
SaqAI TTAA 5 cut(s) 9, 599, 674, 794, 965
SatI GCNGC 4 cut(s) 145, 264, 315, 1133
Sau3AI GATC 7 cut(s) 171, 306, 411, 439, 829, 948, 1083
Sau96I GGNCC 4 cut(s) 228, 369, 574, 1122
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 399, 458
SfaNI GCATC 2 cut(s) 706, 931
SfcI CTRYAG 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 541
SinI GGWCC 3 cut(s) 228, 369, 574
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 927
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 2 cut(s) 116, 1144
SsiI CCGC 8 cut(s) 106, 226, 235, 263, 274, 315, 1132, 1144
SspI AATATT 1 cut(s) 47
SspMI CTAG 2 cut(s) 912, 980
StyD4I CCNGG 2 cut(s) 397, 456
StyI CCWWGG 2 cut(s) 258, 607
TaaI ACNGT 6 cut(s) 71, 248, 376, 1031, 1043, 1110
TaiI ACGT 2 cut(s) 393, 814
TaqI TCGA 7 cut(s) 138, 359, 410, 414, 492, 497, 541
TaqII GACCGA 1 cut(s) 678
TatI WGTACW 1 cut(s) 771
TauI GCSGC 3 cut(s) 266, 317, 1135
TfiI GAWTC 2 cut(s) 915, 991
Tru1I TTAA 5 cut(s) 9, 599, 674, 794, 965
Tru9I TTAA 5 cut(s) 9, 599, 674, 794, 965
TscAI CASTG 3 cut(s) 528, 771, 1036
TseFI GTSAC 2 cut(s) 1031, 1051
TseI GCWGC 1 cut(s) 144
Tsp45I GTSAC 2 cut(s) 1031, 1051
TspDTI ATGAA 4 cut(s) 172, 643, 719, 1115
TspRI CASTG 3 cut(s) 528, 771, 1036
VpaK11BI GGWCC 3 cut(s) 228, 369, 574
XapI RAATTY 4 cut(s) 30, 402, 670, 680
XceI RCATGY 3 cut(s) 116, 367, 1144
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 497
XmnI GAANNNNTTC 2 cut(s) 561, 1109
XspI CTAG 2 cut(s) 912, 980
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.