pycom01g22890

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008217
Physical Location & Seq
Forward (+)
20344567 .. 20346221
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGGGCTGCTATTGTTCAAAGGAAGCTAAAACGCCTGGCTACGAGGACCCTAAGATTCTTGCTGCCGCGACACCTTTTACTGTGAACGAAGTAGAAGCCTTGTATGAGCTTTTTAAGAAATTGAGCAGTTCGATATTTGATGATGGACTTATCCACAAGGAAGAATTTCAGCTCGCGCTCTTCAGGAACAGAAATCGGAGCAATCTTTTTGCAGACAGGATTTTTGACTTATTTGACATCAAGCGCAATGGGGTTATCGAGTTTGGAGAATTTGTTCGATCATTAGGTGTCTTTCACCCCAATGCACCTGTCGAAGACAAAATTTCATTTGCTTTTAGACTGTATAATCTGAGACAGACAGGGTACATTGAGCGAGAGGAGTTGAAGGAGATGGTACTGGCACTTCTGCACGAATCTGATCTGGTACTCTCGGATGATGTCATCGAAGTGATTGTGGATAGGACATACAGTGACGCAGATAAGAAAGGCGATGGGCGGATTGATGAAGAAGAGTGGAAGGAATTTGTGATGAAGCATCCGTCTATGATCAAGAATATGACTCTCCCGTATTTAAAGGATATATCAGTAGCGTTTCCGAGCTTTGTGATGACTTCTGAAGTTGAAGATTCAGAAATATGA

Protein Analysis

213

Amino Acids

24.47

Weight (kDa)

4.77

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 105 - 176 6e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 68, 176
AciI CCGC 2 cut(s) 66, 496
AcsI RAATTY 4 cut(s) 164, 269, 321, 521
AcuI CTGAAG 2 cut(s) 166, 636
AfaI GTAC 3 cut(s) 365, 396, 426
AgsI TTSAA 3 cut(s) 18, 385, 623
AjnI CCWGG 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 4 cut(s) 26, 109, 172, 600
AluI AGCT 4 cut(s) 26, 109, 172, 600
Alw26I GTCTC 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 6, 62
ApoI RAATTY 4 cut(s) 164, 269, 321, 521
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
Asp700I GAANNNNTTC 2 cut(s) 165, 273
AspLEI GCGC 2 cut(s) 178, 246
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 49
BccI CCATC 3 cut(s) 137, 385, 485
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 346
BisI GCNGC 3 cut(s) 7, 63, 66
BlsI GCNGC 3 cut(s) 8, 64, 67
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 544
BpiI GAAGAC 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse1I ACTGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 253
BseBI CCWGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 439, 535
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 1 cut(s) 49
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 68, 176
BsmAI GTCTC 1 cut(s) 346
Bsp143I GATC 3 cut(s) 278, 418, 546
BspACI CCGC 2 cut(s) 66, 496
BspCNI CTCAG 1 cut(s) 342
BspFNI CGCG 2 cut(s) 68, 176
BspLI GGNNCC 1 cut(s) 48
BspQI GCTCTTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 253
BsrI ACTGG 1 cut(s) 402
BssMI GATC 3 cut(s) 278, 418, 546
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 3 cut(s) 82, 342, 470
Bst6I CTCTTC 2 cut(s) 185, 504
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 51, 350
BstF5I GGATG 2 cut(s) 439, 535
BstFNI CGCG 2 cut(s) 68, 176
BstHHI GCGC 2 cut(s) 178, 246
BstKTI GATC 3 cut(s) 281, 421, 549
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 3 cut(s) 278, 418, 546
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstUI CGCG 2 cut(s) 68, 176
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 2 cut(s) 439, 535
BtsIMutI CAGTG 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 178, 246
Cfr13I GGNCC 1 cut(s) 46
CseI GACGC 1 cut(s) 482
Csp6I GTAC 3 cut(s) 364, 395, 425
CviJI RGCY 7 cut(s) 6, 26, 39, 98, 109, 172, 600
CviKI_1 RGCY 7 cut(s) 6, 26, 39, 98, 109, 172, 600
CviQI GTAC 3 cut(s) 364, 395, 425
DdeI CTNAG 2 cut(s) 51, 350
DpnI GATC 3 cut(s) 280, 420, 548
DpnII GATC 3 cut(s) 278, 418, 546
DraI TTTAAA 1 cut(s) 573
Eam1104I CTCTTC 2 cut(s) 185, 504
EarI CTCTTC 2 cut(s) 185, 504
EciI GGCGGA 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 166, 636
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 34
FaiI YATR 7 cut(s) 105, 345, 466, 545, 557, 581, 637
FbaI TGATCA 1 cut(s) 546
Fnu4HI GCNGC 3 cut(s) 7, 63, 66
FokI GGATG 2 cut(s) 446, 522
Fsp4HI GCNGC 3 cut(s) 7, 63, 66
GlaI GCGC 2 cut(s) 177, 245
GluI GCNGC 3 cut(s) 7, 63, 66
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 178, 246
Hin6I GCGC 2 cut(s) 176, 244
HinP1I GCGC 2 cut(s) 176, 244
HinfI GANTC 4 cut(s) 55, 413, 559, 626
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 7 cut(s) 198, 351, 418, 433, 597, 616, 631
Hpy188III TCNNGA 2 cut(s) 184, 550
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 2 cut(s) 379, 511
HpyCH4III ACNGT 3 cut(s) 82, 342, 470
HpyCH4V TGCA 3 cut(s) 212, 305, 409
HpyF3I CTNAG 2 cut(s) 51, 350
HspAI GCGC 2 cut(s) 176, 244
Ksp22I TGATCA 1 cut(s) 546
Kzo9I GATC 3 cut(s) 278, 418, 546
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 8 cut(s) 21, 48, 169, 202, 321, 345, 383, 407
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 544
MaeIII GTNAC 1 cut(s) 470
MalI GATC 3 cut(s) 280, 420, 548
MboI GATC 3 cut(s) 278, 418, 546
MboII GAAGA 6 cut(s) 172, 173, 326, 518, 521, 635
MluCI AATT 5 cut(s) 119, 164, 269, 321, 521
MlyI GAGTC 1 cut(s) 553
MnlI CCTC 2 cut(s) 37, 370
MroXI GAANNNNTTC 2 cut(s) 165, 273
MseI TTAA 2 cut(s) 114, 572
MslI CAYNNNNRTG 2 cut(s) 300, 446
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 68, 176
NdeII GATC 3 cut(s) 278, 418, 546
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 185
PdmI GAANNNNTTC 2 cut(s) 165, 273
PfeI GAWTC 3 cut(s) 55, 413, 626
PkrI GCNGC 3 cut(s) 8, 64, 67
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 46
PspPPI RGGWCCY 1 cut(s) 46
RsaI GTAC 3 cut(s) 365, 396, 426
RsaNI GTAC 3 cut(s) 364, 395, 425
RseI CAYNNNNRTG 2 cut(s) 300, 446
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 114, 572
SatI GCNGC 3 cut(s) 7, 63, 66
Sau3AI GATC 3 cut(s) 278, 418, 546
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 36
SetI ASST 7 cut(s) 28, 76, 111, 174, 289, 310, 602
SfaNI GCATC 1 cut(s) 544
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 300, 446
Sse9I AATT 5 cut(s) 119, 164, 269, 321, 521
SsiI CCGC 2 cut(s) 66, 496
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 3 cut(s) 82, 342, 470
TaqI TCGA 5 cut(s) 131, 258, 277, 312, 444
TasI AATT 5 cut(s) 119, 164, 269, 321, 521
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 55, 413, 626
Tru1I TTAA 2 cut(s) 114, 572
Tru9I TTAA 2 cut(s) 114, 572
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 6, 62
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 315, 519, 545
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 1 cut(s) 475
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 4 cut(s) 164, 269, 321, 521
XmnI GAANNNNTTC 2 cut(s) 165, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.