pycom01g24160

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009101
Physical Location & Seq
Forward (+)
21179618 .. 21181592
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGAGAGAAAAGTGCTTCTGGTTTGCTGCGCTGTGGGACTCCTGGGGCTGCTATCTGCGGCTACAGGTTTCGGCGCCGAGGGCACGAGAATCAAGGGTTCTCAGGTTCAGTTTGTCTCGAATACTCAATGCGAATACCCTCGGAGTCCGGCTCTTGGACTCGGTGTAACTGCTGCAGTGGCTCTTATGTTAGCTCACATAATTATAAATGTTTCAACCGGGTGCATTTGTTGCAAAAGGAGCCCGCATCCTTCCAACTCTAACTGGACGGTAGCTCTGTTCTGCTTTGTTTTTTCCTGGTTCACATTTGTTATAGCGTTTCTTCTGCTGCTCACCGGTGCTGCACTCAATGACCGACATGGTGTAGAAAGCATGTACTTTGGCAACTACTACTGTTACGTTGTGAAACCGGGAGTGTTTGCCGGAGGTGCCCTTTTGTCATTTGCATGTGTGGTACTCGGAATTGTCTACTACGTAATCTTAAATTCAGCAAAGAACGGTGATACATCATGGGGCGGTTCTGCGCCAACTCAAGGGACAATAGCTATGGGGCAACAACTCCAGATCCCACCGCAGAGTACTACGACCCAAGAACCAGTATTTGTCCATGAAGATACGTATATGAGACGGCAATTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

214

Amino Acids

22.91

Weight (kDa)

8.38

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 151 2.9e-29 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AccB1I GGYRCC 2 cut(s) 74, 428
AccI GTMKAC 1 cut(s) 468
AciI CCGC 4 cut(s) 59, 245, 516, 572
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 484
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 3 cut(s) 377, 456, 580
AfiI CCNNNNNNNGG 2 cut(s) 155, 533
AgeI ACCGGT 1 cut(s) 335
AgsI TTSAA 1 cut(s) 216
AjnI CCWGG 2 cut(s) 42, 296
AluBI AGCT 3 cut(s) 194, 275, 545
AluI AGCT 3 cut(s) 194, 275, 545
Alw26I GTCTC 2 cut(s) 121, 619
AlwI GGATC 1 cut(s) 559
ApeKI GCWGC 5 cut(s) 27, 49, 173, 328, 341
ApoI RAATTY 1 cut(s) 484
AsiGI ACCGGT 1 cut(s) 335
AspLEI GCGC 3 cut(s) 32, 77, 526
AsuC2I CCSGG 2 cut(s) 220, 411
AsuHPI GGTGA 2 cut(s) 325, 512
BaeGI GKGCMC 2 cut(s) 86, 433
BanI GGYRCC 2 cut(s) 74, 428
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 85
BbvI GCAGC 5 cut(s) 14, 36, 160, 315, 328
BciT130I CCWGG 2 cut(s) 44, 298
BcnI CCSGG 2 cut(s) 220, 411
BcoDI GTCTC 2 cut(s) 121, 619
BfmI CTRYAG 2 cut(s) 63, 174
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 6 cut(s) 28, 50, 60, 174, 329, 342
BlsI GCNGC 6 cut(s) 29, 51, 61, 175, 330, 343
BmcAI AGTACT 1 cut(s) 580
Bme1390I CCNGG 4 cut(s) 44, 220, 298, 411
BmiI GGNNCC 3 cut(s) 76, 242, 430
BmrFI CCNGG 4 cut(s) 44, 220, 298, 411
BmsI GCATC 1 cut(s) 256
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 220, 411
BsaAI YACGTR 2 cut(s) 475, 618
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 43, 78, 140
BsaWI WCCGGW 1 cut(s) 335
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 533
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 269, 596
BseBI CCWGG 2 cut(s) 44, 298
BseDI CCNNGG 3 cut(s) 43, 78, 140
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 155, 533
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 269, 596
BseSI GKGCMC 2 cut(s) 86, 433
BseXI GCAGC 5 cut(s) 14, 36, 160, 315, 328
BsgI GTGCAG 1 cut(s) 327
BshNI GGYRCC 2 cut(s) 74, 428
BshTI ACCGGT 1 cut(s) 335
BsiSI CCGG 5 cut(s) 149, 219, 336, 410, 423
BslFI GGGAC 2 cut(s) 51, 550
BslI CCNNNNNNNGG 2 cut(s) 155, 533
BsmAI GTCTC 2 cut(s) 121, 619
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 2 cut(s) 51, 550
Bsp1286I GDGCHC 3 cut(s) 86, 245, 433
Bsp143I GATC 1 cut(s) 564
BspACI CCGC 4 cut(s) 59, 245, 516, 572
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 3 cut(s) 76, 242, 430
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 559
BspT107I GGYRCC 2 cut(s) 74, 428
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 2 cut(s) 269, 596
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 43, 78, 140
BssMI GATC 1 cut(s) 564
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 44, 298
Bst4CI ACNGT 3 cut(s) 271, 395, 500
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 2 cut(s) 475, 618
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 3 cut(s) 32, 77, 526
BstKTI GATC 1 cut(s) 567
BstMAI GTCTC 2 cut(s) 121, 619
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 5 cut(s) 81, 179, 231, 240, 428
BstNI CCWGG 2 cut(s) 44, 298
BstNSI RCATGY 2 cut(s) 376, 450
BstSCI CCNGG 4 cut(s) 42, 218, 296, 409
BstSFI CTRYAG 2 cut(s) 63, 174
BstSLI GKGCMC 2 cut(s) 86, 433
BstSNI TACGTA 2 cut(s) 475, 618
BstV1I GCAGC 5 cut(s) 14, 36, 160, 315, 328
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 245
CfoI GCGC 3 cut(s) 32, 77, 526
Cfr10I RCCGGY 1 cut(s) 335
Csp6I GTAC 3 cut(s) 376, 455, 579
CspAI ACCGGT 1 cut(s) 335
CviAII CATG 6 cut(s) 359, 373, 447, 510, 608, 639
CviJI RGCY 8 cut(s) 49, 62, 152, 182, 194, 243, 275, 545
CviKI_1 RGCY 8 cut(s) 49, 62, 152, 182, 194, 243, 275, 545
CviQI GTAC 3 cut(s) 376, 455, 579
DdeI CTNAG 1 cut(s) 102
DinI GGCGCC 1 cut(s) 76
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Eco105I TACGTA 2 cut(s) 475, 618
Eco24I GRGCYC 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 42, 296
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 6 cut(s) 362, 376, 450, 513, 611, 642
FaqI GGGAC 2 cut(s) 51, 550
FatI CATG 6 cut(s) 358, 372, 446, 509, 607, 638
FauI CCCGC 1 cut(s) 252
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 28, 50, 60, 174, 329, 342
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 6 cut(s) 28, 50, 60, 174, 329, 342
GlaI GCGC 3 cut(s) 31, 76, 525
GluI GCNGC 6 cut(s) 28, 50, 60, 174, 329, 342
GsuI CTGGAG 1 cut(s) 545
HaeII RGCGCY 1 cut(s) 78
HapII CCGG 5 cut(s) 149, 219, 336, 410, 423
HhaI GCGC 3 cut(s) 32, 77, 526
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 6 cut(s) 362, 376, 450, 513, 611, 642
Hin6I GCGC 3 cut(s) 30, 75, 524
HinP1I GCGC 3 cut(s) 30, 75, 524
HinfI GANTC 4 cut(s) 39, 90, 145, 159
HpaII CCGG 5 cut(s) 149, 219, 336, 410, 423
HphI GGTGA 2 cut(s) 325, 512
Hpy166II GTNNAC 2 cut(s) 303, 469
Hpy188I TCNGA 2 cut(s) 144, 461
Hpy188III TCNNGA 2 cut(s) 118, 562
Hpy8I GTNNAC 2 cut(s) 303, 469
HpyAV CCTTC 1 cut(s) 261
HpyCH4III ACNGT 3 cut(s) 271, 395, 500
HpyCH4IV ACGT 3 cut(s) 399, 474, 617
HpyCH4V TGCA 5 cut(s) 176, 225, 234, 344, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 179, 231, 240, 428
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 3 cut(s) 399, 474, 617
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 6 cut(s) 362, 376, 450, 513, 611, 642
HspAI GCGC 3 cut(s) 30, 75, 524
KasI GGCGCC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 564
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 5 cut(s) 14, 36, 160, 315, 328
LweI GCATC 1 cut(s) 256
MaeII ACGT 3 cut(s) 399, 474, 617
MaeIII GTNAC 2 cut(s) 166, 395
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 2 cut(s) 314, 623
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 3 cut(s) 86, 245, 433
MluCI AATT 4 cut(s) 201, 462, 484, 632
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 3 cut(s) 33, 153, 154
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 3 cut(s) 73, 150, 419
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 445
MspI CCGG 5 cut(s) 149, 219, 336, 410, 423
MspR9I CCNGG 4 cut(s) 44, 220, 298, 411
MvaI CCWGG 2 cut(s) 44, 298
MwoI GCNNNNNNNGC 5 cut(s) 81, 179, 231, 240, 428
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 2 cut(s) 220, 411
NdeII GATC 1 cut(s) 564
NlaIII CATG 6 cut(s) 362, 376, 450, 513, 611, 642
NlaIV GGNNCC 3 cut(s) 76, 242, 430
NmeAIII GCCGAG 1 cut(s) 103
NspI RCATGY 2 cut(s) 376, 450
PfeI GAWTC 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 335
PkrI GCNGC 6 cut(s) 29, 51, 61, 175, 330, 343
PleI GAGTC 3 cut(s) 33, 153, 153
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 3 cut(s) 33, 153, 153
Ppu21I YACGTR 2 cut(s) 475, 618
PsiI TTATAA 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 42, 296
PspGI CCWGG 2 cut(s) 42, 296
PspN4I GGNNCC 3 cut(s) 76, 242, 430
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 3 cut(s) 377, 456, 580
RsaNI GTAC 3 cut(s) 376, 455, 579
RseI CAYNNNNRTG 1 cut(s) 445
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 6 cut(s) 28, 50, 60, 174, 329, 342
Sau3AI GATC 1 cut(s) 564
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 3 cut(s) 33, 153, 154
ScrFI CCNGG 4 cut(s) 44, 220, 298, 411
SduI GDGCHC 3 cut(s) 86, 245, 433
SetI ASST 9 cut(s) 70, 108, 196, 277, 402, 430, 477, 547, 620
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 63, 174
SfoI GGCGCC 1 cut(s) 76
SgrAI CRCCGGYG 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
SnaBI TACGTA 2 cut(s) 475, 618
Sse9I AATT 4 cut(s) 201, 462, 484, 632
SsiI CCGC 4 cut(s) 59, 245, 516, 572
SspDI GGCGCC 1 cut(s) 74
StyD4I CCNGG 4 cut(s) 42, 218, 296, 409
TaaI ACNGT 3 cut(s) 271, 395, 500
TaiI ACGT 3 cut(s) 402, 477, 620
TaqI TCGA 1 cut(s) 119
TaqII GACCGA 1 cut(s) 369
TasI AATT 4 cut(s) 201, 462, 484, 632
TatI WGTACW 2 cut(s) 375, 578
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 1 cut(s) 183
TseI GCWGC 5 cut(s) 27, 49, 173, 328, 341
TspDTI ATGAA 1 cut(s) 624
TspRI CASTG 1 cut(s) 183
XapI RAATTY 1 cut(s) 484
XceI RCATGY 2 cut(s) 376, 450
XmiI GTMKAC 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.