pycom02g03140

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009140
Physical Location & Seq
Forward (+)
2047228 .. 2050205
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGATAATAAGAAGAAGGTGGCGGTTCCGTTGGTCTGCCACGGTCATTCGCGTCCGGTGGTCGATCTGTTTTACAGTCCGGTTACTCCTGATGGGTTCTTTCTCATCAGTGCCAGTAAGGATTCGAGTCCCATGCTGAGAAATGGAGAGACTGGAGATTGGGTTGGAACATTTGAAGGGCATAAAGGCGCAGTGTGGAGTTGCTGCTTGGATACCAACGCCCTGCGTGCAGCAACTGCTTCTGCAGATTTCTCTGCGAAAGTTTGGGATGCATTAACAGGAGATGTTCTACACTCATTTGAACACAAGCACATTGTTCGAGCCTGTGCTTTTGCAGAGGACACACACCTTTTGCTTACTGGAGGAGTAGAGAAAGTTATTCGTATATATGATTTGAATCGTCCAGATGCTCCTCCAAGAGAAGTGGATAAATCTCCTGGTTCAGTCAGAACTGCTGCATGGCTCCACAGTGACCAGACCATACTAAGTTCTTGTACTGATATGGGAGGTGTCAGATTATGGGATGTAAGAACTGGTAAAATAGTTCAAACGCTTGAAACCAAATCATCTGTGACCAGTTCTGAAGTGAGTCAAGATGGTCGTTATATCACTACTGCTGATGGGTCTACTGTGAAGTTTTGGGATGCAAATTACTTTGGGTTGGTGAAGAGCTACAACATGCCATGCACAGTAGAATCAGCTTCGCTGGAACCAAAGTTCGGGAACAAGTTCATTGCCGGGGGAGAAGACATGTGGGTTCATGTACTTGATTTCCATACTGGTGAAGAGATCGCATGCAACAAGGGTCACCACGGTCCAGTTCACTGTTTGCGATTCTCGCCAGGAGGGGAATCATACGCCTCAGGATCTGAAGATGGCACCATTAGAATATGGCAGACCGGGCCCTTGACTCCAGATGAAGCTGAGGCTAATGGGTCAGTTGGAAAGGTGAAGGTACCCGCAGAAGAGGTTTCTCACAAGATCGAGGGGTTCCACATCACAGAAGAGGGAAAAGCGAAAGAGAAGGAAGAGGCAGCGGTGAACGAGTGA

Protein Analysis

350

Amino Acids

37.92

Weight (kDa)

5.52

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 10 - 298 7.9e-45 EIF3I
Beta-prop_WDR3_2nd PF25172 13 - 131 4.3e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 13 - 133 3.6e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 14 - 226 5.7e-28 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 30 - 166 1.3e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 34 - 215 1.3e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 34 - 226 5.4e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 35 - 133 1.3e-06 G protein beta WD-40 repeat protein
WD40 PF00400 55 - 90 5.6e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 57 - 216 1.1e-09 G protein beta WD-40 repeat protein
WDR55 PF24796 99 - 209 2.2e-08 WDR55
Beta-prop_TEP1_2nd PF25047 101 - 221 3.7e-11 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 101 - 239 2.8e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 102 - 300 3.1e-32 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 106 - 218 4.1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 147 - 215 3.9e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 158 - 298 5.8e-08 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 171 - 305 9.4e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 191 - 300 3.8e-08 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 202 - 300 4.8e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 231 - 301 3.1e-09 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 241 - 300 5e-11 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 241 - 299 5.2e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 244 - 300 5.7e-09 WDR5 beta-propeller domain
WD40 PF00400 264 - 298 6.2e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 955
AccB1I GGYRCC 2 cut(s) 878, 955
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 52
AciI CCGC 3 cut(s) 23, 960, 1037
AclWI GGATC 1 cut(s) 874
AcuI CTGAAG 2 cut(s) 603, 891
AfaI GTAC 3 cut(s) 496, 765, 957
AfiI CCNNNNNNNGG 1 cut(s) 719
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 5 cut(s) 176, 302, 397, 548, 557
AjnI CCWGG 2 cut(s) 436, 841
AloI GAACNNNNNNTCC 2 cut(s) 701, 733
AluBI AGCT 3 cut(s) 672, 701, 923
AluI AGCT 3 cut(s) 672, 701, 923
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 874
AlwNI CAGNNNCTG 2 cut(s) 236, 869
AoxI GGCC 1 cut(s) 902
ApaI GGGCCC 1 cut(s) 906
ApeKI GCWGC 4 cut(s) 204, 230, 455, 1034
Asp700I GAANNNNTTC 1 cut(s) 728
Asp718I GGTACC 1 cut(s) 955
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 815, 902, 903
AsuC2I CCSGG 2 cut(s) 739, 901
AsuHPI GGTGA 4 cut(s) 676, 794, 800, 961
AvaII GGWCC 1 cut(s) 815
AxyI CCTNAGG 1 cut(s) 862
BaeGI GKGCMC 1 cut(s) 906
BanI GGYRCC 2 cut(s) 878, 955
BanII GRGCYC 1 cut(s) 906
BbsI GAAGAC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 4 cut(s) 191, 242, 442, 1046
BccI CCATC 4 cut(s) 86, 590, 614, 869
BcgI CGANNNNNNTGC 4 cut(s) 115, 149, 299, 333
BciT130I CCWGG 2 cut(s) 438, 843
BciVI GTATCC 1 cut(s) 205
BcnI CCSGG 2 cut(s) 739, 901
BcoDI GTCTC 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 1 cut(s) 205
BisI GCNGC 4 cut(s) 205, 231, 456, 1035
BlsI GCNGC 4 cut(s) 206, 232, 457, 1036
Bme1390I CCNGG 4 cut(s) 438, 739, 843, 901
Bme18I GGWCC 1 cut(s) 815
BmgT120I GGNCC 3 cut(s) 815, 902, 903
BmiI GGNNCC 7 cut(s) 27, 464, 711, 880, 904, 957, 992
BmrFI CCNGG 4 cut(s) 438, 739, 843, 901
BmsI GCATC 3 cut(s) 259, 397, 634
BpiI GAAGAC 1 cut(s) 753
BpmI CTGGAG 3 cut(s) 174, 381, 897
Bpu10I CCTNAGC 1 cut(s) 924
BpuMI CCSGG 2 cut(s) 739, 901
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 40, 738, 811
BsaWI WCCGGW 2 cut(s) 55, 79
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 7 cut(s) 114, 157, 364, 538, 576, 784, 818
Bse21I CCTNAGG 1 cut(s) 862
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 438, 843
BseDI CCNNGG 3 cut(s) 40, 738, 811
BseGI GGATG 3 cut(s) 274, 529, 649
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 3 cut(s) 128, 876, 915
BseNI ACTGG 7 cut(s) 114, 157, 364, 538, 576, 784, 818
BseRI GAGGAG 2 cut(s) 378, 402
BseSI GKGCMC 1 cut(s) 906
BseXI GCAGC 4 cut(s) 191, 242, 442, 1046
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 1 cut(s) 904
BshNI GGYRCC 2 cut(s) 878, 955
BsiSI CCGG 4 cut(s) 56, 80, 738, 900
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 904
Bsp120I GGGCCC 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 906
Bsp143I GATC 4 cut(s) 64, 789, 866, 981
BspACI CCGC 3 cut(s) 23, 960, 1037
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 3 cut(s) 129, 875, 916
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 7 cut(s) 27, 464, 711, 880, 904, 957, 992
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 1 cut(s) 874
BspQI GCTCTTC 1 cut(s) 662
BspT107I GGYRCC 2 cut(s) 878, 955
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 7 cut(s) 114, 157, 364, 538, 576, 784, 818
BssECI CCNNGG 3 cut(s) 40, 738, 811
BssMI GATC 4 cut(s) 64, 789, 866, 981
Bst2UI CCWGG 2 cut(s) 438, 843
Bst4CI ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
Bst6I CTCTTC 5 cut(s) 662, 780, 960, 999, 1023
BstAPI GCANNNNNTGC 1 cut(s) 236
BstC8I GCNNGC 2 cut(s) 228, 796
BstDEI CTNAG 4 cut(s) 137, 485, 862, 924
BstDSI CCRYGG 2 cut(s) 40, 811
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 3 cut(s) 274, 529, 649
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 4 cut(s) 67, 792, 869, 984
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 4 cut(s) 64, 789, 866, 981
BstMWI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 901
BstNI CCWGG 2 cut(s) 438, 843
BstNSI RCATGY 3 cut(s) 682, 754, 798
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 436, 737, 841, 899
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 906
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 4 cut(s) 191, 242, 442, 1046
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 1 cut(s) 866
BstYI RGATCY 1 cut(s) 866
Bsu36I CCTNAGG 1 cut(s) 862
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 1 cut(s) 904
BtgI CCRYGG 2 cut(s) 40, 811
BtsCI GGATG 3 cut(s) 274, 529, 649
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 115, 198, 475, 823
Cac8I GCNNGC 2 cut(s) 228, 796
CaiI CAGNNNCTG 2 cut(s) 236, 869
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 815, 902, 903
CseI GACGC 1 cut(s) 41
Csp6I GTAC 3 cut(s) 495, 764, 956
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 7 cut(s) 133, 459, 679, 684, 751, 761, 795
CviJI RGCY 7 cut(s) 323, 463, 672, 701, 904, 923, 929
CviKI_1 RGCY 7 cut(s) 323, 463, 672, 701, 904, 923, 929
CviQI GTAC 3 cut(s) 495, 764, 956
DdeI CTNAG 4 cut(s) 137, 485, 862, 924
DpnI GATC 4 cut(s) 66, 791, 868, 983
DpnII GATC 4 cut(s) 64, 789, 866, 981
Eam1104I CTCTTC 5 cut(s) 662, 780, 960, 999, 1023
EarI CTCTTC 5 cut(s) 662, 780, 960, 999, 1023
Eco24I GRGCYC 1 cut(s) 906
Eco47I GGWCC 1 cut(s) 815
Eco57I CTGAAG 2 cut(s) 603, 891
Eco81I CCTNAGG 1 cut(s) 862
Eco91I GGTNACC 1 cut(s) 806
EcoO109I RGGNCCY 1 cut(s) 903
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 2 cut(s) 436, 841
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 906
FaeI CATG 7 cut(s) 136, 462, 682, 687, 754, 764, 798
FaqI GGGAC 1 cut(s) 114
FatI CATG 7 cut(s) 132, 458, 678, 683, 750, 760, 794
FauI CCCGC 1 cut(s) 967
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 4 cut(s) 205, 231, 456, 1035
FokI GGATG 3 cut(s) 281, 536, 656
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 4 cut(s) 205, 231, 456, 1035
GlaI GCGC 1 cut(s) 190
GluI GCNGC 4 cut(s) 205, 231, 456, 1035
GsuI CTGGAG 3 cut(s) 174, 381, 897
HaeIII GGCC 1 cut(s) 904
HapII CCGG 4 cut(s) 56, 80, 738, 900
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 191
Hin1II CATG 7 cut(s) 136, 462, 682, 687, 754, 764, 798
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 8 cut(s) 122, 127, 397, 589, 695, 834, 851, 910
HpaII CCGG 4 cut(s) 56, 80, 738, 900
HphI GGTGA 4 cut(s) 676, 794, 800, 961
Hpy166II GTNNAC 3 cut(s) 627, 823, 1042
Hpy188I TCNGA 4 cut(s) 449, 515, 583, 871
Hpy188III TCNNGA 6 cut(s) 89, 404, 593, 721, 864, 914
Hpy8I GTNNAC 3 cut(s) 627, 823, 1042
HpyAV CCTTC 4 cut(s) 10, 170, 946, 1018
HpyCH4III ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
HpyCH4V TGCA 8 cut(s) 230, 245, 272, 335, 458, 647, 687, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 901
HpyF3I CTNAG 4 cut(s) 137, 485, 862, 924
Hsp92II CATG 7 cut(s) 136, 462, 682, 687, 754, 764, 798
HspAI GCGC 1 cut(s) 189
KpnI GGTACC 1 cut(s) 959
Kzo9I GATC 4 cut(s) 64, 789, 866, 981
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 2 cut(s) 415, 468
Lsp1109I GCAGC 4 cut(s) 191, 242, 442, 1046
LweI GCATC 3 cut(s) 259, 397, 634
MaeIII GTNAC 4 cut(s) 82, 470, 571, 806
MalI GATC 4 cut(s) 66, 791, 868, 983
MboI GATC 4 cut(s) 64, 789, 866, 981
MboII GAAGA 8 cut(s) 25, 679, 758, 797, 884, 977, 1016, 1040
MflI RGATCY 1 cut(s) 866
MhlI GDGCHC 1 cut(s) 906
MluCI AATT 1 cut(s) 649
MlyI GAGTC 3 cut(s) 136, 598, 904
MmeI TCCRAC 2 cut(s) 145, 922
Mph1103I ATGCAT 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 728
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 4 cut(s) 56, 80, 738, 900
MspR9I CCNGG 4 cut(s) 438, 739, 843, 901
MvaI CCWGG 2 cut(s) 438, 843
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 901
NciI CCSGG 2 cut(s) 739, 901
NdeII GATC 4 cut(s) 64, 789, 866, 981
NlaIII CATG 7 cut(s) 136, 462, 682, 687, 754, 764, 798
NlaIV GGNNCC 7 cut(s) 27, 464, 711, 880, 904, 957, 992
NmuCI GTSAC 3 cut(s) 470, 571, 806
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 3 cut(s) 682, 754, 798
PaeI GCATGC 1 cut(s) 798
PciI ACATGT 1 cut(s) 750
PciSI GCTCTTC 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 5 cut(s) 122, 397, 695, 834, 851
PkrI GCNGC 4 cut(s) 206, 232, 457, 1036
PleI GAGTC 3 cut(s) 135, 597, 904
PpsI GAGTC 3 cut(s) 135, 597, 904
PscI ACATGT 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 436, 841
PspEI GGTNACC 1 cut(s) 806
PspGI CCWGG 2 cut(s) 436, 841
PspN4I GGNNCC 7 cut(s) 27, 464, 711, 880, 904, 957, 992
PspOMI GGGCCC 1 cut(s) 902
PspPI GGNCC 3 cut(s) 815, 902, 903
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 2 cut(s) 236, 869
PsuI RGATCY 1 cut(s) 866
RsaI GTAC 3 cut(s) 496, 765, 957
RsaNI GTAC 3 cut(s) 495, 764, 956
RseI CAYNNNNRTG 1 cut(s) 780
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 4 cut(s) 205, 231, 456, 1035
Sau3AI GATC 4 cut(s) 64, 789, 866, 981
Sau96I GGNCC 3 cut(s) 815, 902, 903
SchI GAGTC 3 cut(s) 136, 598, 904
ScrFI CCNGG 4 cut(s) 438, 739, 843, 901
SduI GDGCHC 1 cut(s) 906
SetI ASST 9 cut(s) 21, 351, 511, 674, 703, 925, 951, 957, 972
SfaNI GCATC 3 cut(s) 259, 397, 634
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 1 cut(s) 780
SphI GCATGC 1 cut(s) 798
Sse9I AATT 1 cut(s) 649
SsiI CCGC 3 cut(s) 23, 960, 1037
StyD4I CCNGG 4 cut(s) 436, 737, 841, 899
TaaI ACNGT 7 cut(s) 44, 77, 470, 631, 691, 815, 827
TaqI TCGA 4 cut(s) 63, 125, 319, 984
TasI AATT 1 cut(s) 649
TatI WGTACW 2 cut(s) 494, 763
TfiI GAWTC 5 cut(s) 122, 397, 695, 834, 851
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 4 cut(s) 115, 198, 475, 830
TseFI GTSAC 3 cut(s) 470, 571, 806
TseI GCWGC 4 cut(s) 204, 230, 455, 1034
Tsp45I GTSAC 3 cut(s) 470, 571, 806
TspDTI ATGAA 3 cut(s) 721, 749, 933
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 4 cut(s) 115, 198, 475, 830
VpaK11BI GGWCC 1 cut(s) 815
XceI RCATGY 3 cut(s) 682, 754, 798
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.