pycom02g06940

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011508
Physical Location & Seq
Reverse (-)
4805157 .. 4806892
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGCCCCTTTATACTAATTCCATGTCGTGGAATCCAAGTGAACAAGTTCCACAACTTTTATCCAAAGATGTAAGCTTCAAAGTGAAATTTCCGCCACAAGTTCATCATGGCGCAAAGCATTTAGGACTCCAACTACAAACAGACCACGGATCATCATCCATAACCCAATCAATCGGTCACTCTCAAGATCCAAGCAATTCATCAGATTCTGGCGAAGTTCAAACATGTGATAAGGATGCTGAAGGCAACATGAAGCCAGTTCTCTTGCAGAGTAATCAAGATTTCATTTTGAATCCTTCTCAAGTTAGTCACGGAAATTTAGTGGGTTGTGTACCATATCCTTATGTCGACCCTTACTTCGGCGGGTTCTTGACTGCATATGGACCACAGGCGATGTCCCATATGGTTGGGATGGCACCTACACGAGTTCTATTGCCTCCCGATCTGGCACAAGATGGACCCATCTACGTCAATGCAAAACAATACCACGGAATCCTCAGAAGGAGACAGTCCCGTGCAAAGTTGGAGGCTCGTAACAAACTTCTCAAAGCTCGAAAGCCTTATCTCCATGAGTCTCGCCATCTTCATGCCGTGAATAGGGTAAGGGGATCTGGTGGACGTTTTCTCAGCACAAAACGACAGTCAGACCAGAACCCCTCCAGTAACACCCACCATGTTTCGGATTCCATCAGCTCACACCAGAAAGATACAGGGGGCACAGAAGGTAATCACTCGGGAAGCAGTGATTTCCTCCCTAGTGTTGCACTCCACTCAGACATGACTAGTGTTTCCAACACCAATGACAACTTTCAGCAACCAGATCGCAGATTCTCAGGCATCCCTCCCCATATGACTGGAGCAATGCAGTTCCCTGGCGGGTTTATGCATGCTGGAAATCAGCACTGTGCTTCTGTTGTCCGGTGA

Protein Analysis

308

Amino Acids

33.64

Weight (kDa)

9.1

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 154 - 208 2.4e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 27
AccI GTMKAC 1 cut(s) 348
AciI CCGC 3 cut(s) 92, 363, 876
AclWI GGATC 3 cut(s) 157, 182, 616
AcsI RAATTY 2 cut(s) 86, 316
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 4 cut(s) 27, 359, 597, 679
AflIII ACRYGT 1 cut(s) 224
AgsI TTSAA 3 cut(s) 79, 221, 292
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 1 cut(s) 871
AluBI AGCT 3 cut(s) 75, 551, 693
AluI AGCT 3 cut(s) 75, 551, 693
Alw26I GTCTC 2 cut(s) 499, 579
AlwI GGATC 3 cut(s) 157, 182, 616
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 733
ApoI RAATTY 2 cut(s) 86, 316
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 626, 658
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 383, 458
AvaI CYCGRG 1 cut(s) 733
AvaII GGWCC 2 cut(s) 383, 458
BaeGI GKGCMC 1 cut(s) 719
BanI GGYRCC 1 cut(s) 415
BauI CACGAG 1 cut(s) 423
BccI CCATC 5 cut(s) 406, 449, 470, 588, 695
BceAI ACGGC 1 cut(s) 575
BcgI CGANNNNNNTGC 2 cut(s) 803, 837
BciT130I CCWGG 1 cut(s) 873
BcoDI GTCTC 2 cut(s) 499, 579
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 2 cut(s) 756, 783
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 2 cut(s) 383, 458
BmeT110I CYCGRG 1 cut(s) 733
BmgT120I GGNCC 2 cut(s) 383, 458
BmiI GGNNCC 2 cut(s) 417, 460
BmrFI CCNGG 1 cut(s) 873
BmsI GCATC 2 cut(s) 226, 846
BpmI CTGGAG 2 cut(s) 643, 876
BpuEI CTTGAG 2 cut(s) 168, 285
BsaBI GATNNNNATC 1 cut(s) 154
BsaJI CCNNGG 3 cut(s) 145, 487, 871
BsaWI WCCGGW 1 cut(s) 918
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 359, 597, 679
Bse1I ACTGG 3 cut(s) 257, 660, 859
Bse3DI GCAATG 1 cut(s) 867
Bse8I GATNNNNATC 1 cut(s) 154
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 145, 487, 871
BseGI GGATG 4 cut(s) 155, 241, 417, 837
BseJI GATNNNNATC 1 cut(s) 154
BseLI CCNNNNNNNGG 4 cut(s) 27, 359, 597, 679
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 4 cut(s) 511, 640, 786, 846
BseNI ACTGG 3 cut(s) 257, 660, 859
BseSI GKGCMC 1 cut(s) 719
BshNI GGYRCC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 1 cut(s) 919
BslFI GGGAC 2 cut(s) 382, 496
BslI CCNNNNNNNGG 4 cut(s) 27, 359, 597, 679
BsmAI GTCTC 2 cut(s) 499, 579
BsmFI GGGAC 2 cut(s) 382, 496
BsoBI CYCGRG 1 cut(s) 733
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 5 cut(s) 149, 187, 442, 608, 820
BspACI CCGC 3 cut(s) 92, 363, 876
BspCNI CTCAG 4 cut(s) 510, 639, 785, 845
BspLI GGNNCC 2 cut(s) 417, 460
BspPI GGATC 3 cut(s) 157, 182, 616
BspT107I GGYRCC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 3 cut(s) 257, 660, 859
BssECI CCNNGG 3 cut(s) 145, 487, 871
BssMI GATC 5 cut(s) 149, 187, 442, 608, 820
BssSI CACGAG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 3 cut(s) 510, 642, 905
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 4 cut(s) 497, 626, 772, 832
BstDSI CCRYGG 2 cut(s) 145, 487
BstF5I GGATG 4 cut(s) 155, 241, 417, 837
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 5 cut(s) 152, 190, 445, 611, 823
BstMAI GTCTC 2 cut(s) 499, 579
BstMBI GATC 5 cut(s) 149, 187, 442, 608, 820
BstNI CCWGG 1 cut(s) 873
BstNSI RCATGY 2 cut(s) 228, 890
BstSCI CCNGG 1 cut(s) 871
BstSLI GKGCMC 1 cut(s) 719
BstX2I RGATCY 2 cut(s) 187, 608
BstXI CCANNNNNNTGG 2 cut(s) 407, 854
BstYI RGATCY 2 cut(s) 187, 608
BtgI CCRYGG 2 cut(s) 145, 487
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 4 cut(s) 155, 241, 417, 837
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 2 cut(s) 748, 901
Cac8I GCNNGC 1 cut(s) 888
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 383, 458
Csp6I GTAC 1 cut(s) 332
CviAII CATG 9 cut(s) 22, 107, 225, 250, 569, 587, 674, 778, 887
CviJI RGCY 6 cut(s) 75, 256, 530, 551, 559, 693
CviKI_1 RGCY 6 cut(s) 75, 256, 530, 551, 559, 693
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 4 cut(s) 497, 626, 772, 832
DpnI GATC 5 cut(s) 151, 189, 444, 610, 822
DpnII GATC 5 cut(s) 149, 187, 442, 608, 820
EciI GGCGGA 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 383, 458
Eco57I CTGAAG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 871
EcoT22I ATGCAT 1 cut(s) 888
FaeI CATG 9 cut(s) 25, 110, 228, 253, 572, 590, 677, 781, 890
FaqI GGGAC 2 cut(s) 382, 496
FatI CATG 9 cut(s) 21, 106, 224, 249, 568, 586, 673, 777, 886
FauI CCCGC 2 cut(s) 356, 869
FauNDI CATATG 3 cut(s) 379, 402, 849
FblI GTMKAC 1 cut(s) 348
FokI GGATG 4 cut(s) 142, 248, 424, 824
FspBI CTAG 2 cut(s) 756, 783
GlaI GCGC 1 cut(s) 112
GsuI CTGGAG 2 cut(s) 643, 876
HapII CCGG 1 cut(s) 919
HhaI GCGC 1 cut(s) 113
Hin1II CATG 9 cut(s) 25, 110, 228, 253, 572, 590, 677, 781, 890
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 73
HinfI GANTC 8 cut(s) 31, 126, 206, 292, 492, 572, 683, 828
HpaII CCGG 1 cut(s) 919
Hpy166II GTNNAC 4 cut(s) 41, 332, 349, 617
Hpy188I TCNGA 5 cut(s) 205, 500, 646, 682, 775
Hpy188III TCNNGA 5 cut(s) 185, 278, 370, 440, 735
Hpy8I GTNNAC 4 cut(s) 41, 332, 349, 617
HpyAV CCTTC 4 cut(s) 236, 306, 495, 716
HpyCH4III ACNGT 3 cut(s) 510, 642, 905
HpyCH4IV ACGT 2 cut(s) 468, 619
HpyCH4V TGCA 7 cut(s) 268, 377, 476, 518, 764, 865, 886
HpyF3I CTNAG 4 cut(s) 497, 626, 772, 832
HpySE526I ACGT 2 cut(s) 468, 619
Hsp92II CATG 9 cut(s) 25, 110, 228, 253, 572, 590, 677, 781, 890
HspAI GCGC 1 cut(s) 111
Kzo9I GATC 5 cut(s) 149, 187, 442, 608, 820
LmnI GCTCC 1 cut(s) 857
LweI GCATC 2 cut(s) 226, 846
MaeI CTAG 2 cut(s) 756, 783
MaeII ACGT 2 cut(s) 468, 619
MaeIII GTNAC 4 cut(s) 176, 308, 533, 662
MalI GATC 5 cut(s) 151, 189, 444, 610, 822
MboI GATC 5 cut(s) 149, 187, 442, 608, 820
MboII GAAGA 1 cut(s) 575
MflI RGATCY 2 cut(s) 187, 608
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 4 cut(s) 16, 86, 196, 316
MlyI GAGTC 2 cut(s) 120, 581
MmeI TCCRAC 3 cut(s) 154, 504, 816
MnlI CCTC 6 cut(s) 447, 506, 520, 667, 761, 852
Mph1103I ATGCAT 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 1 cut(s) 919
MspR9I CCNGG 1 cut(s) 873
MvaI CCWGG 1 cut(s) 873
NdeI CATATG 3 cut(s) 379, 402, 849
NdeII GATC 5 cut(s) 149, 187, 442, 608, 820
NlaIII CATG 9 cut(s) 25, 110, 228, 253, 572, 590, 677, 781, 890
NlaIV GGNNCC 2 cut(s) 417, 460
NmuCI GTSAC 2 cut(s) 176, 308
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 2 cut(s) 228, 890
PaeI GCATGC 1 cut(s) 890
PciI ACATGT 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 6 cut(s) 31, 206, 292, 492, 683, 828
PflMI CCANNNNNTGG 1 cut(s) 27
PleI GAGTC 2 cut(s) 120, 580
PpsI GAGTC 2 cut(s) 120, 580
PscI ACATGT 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 871
PspGI CCWGG 1 cut(s) 871
PspN4I GGNNCC 2 cut(s) 417, 460
PspPI GGNCC 2 cut(s) 383, 458
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 2 cut(s) 187, 608
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 585
SalI GTCGAC 1 cut(s) 347
Sau3AI GATC 5 cut(s) 149, 187, 442, 608, 820
Sau96I GGNCC 2 cut(s) 383, 458
SchI GAGTC 2 cut(s) 120, 581
ScrFI CCNGG 1 cut(s) 873
SduI GDGCHC 1 cut(s) 719
SetI ASST 7 cut(s) 77, 421, 471, 553, 622, 695, 727
SfaNI GCATC 2 cut(s) 226, 846
SinI GGWCC 2 cut(s) 383, 458
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 183, 300
SmoI CTYRAG 2 cut(s) 183, 300
SpeI ACTAGT 1 cut(s) 782
SphI GCATGC 1 cut(s) 890
Sse9I AATT 4 cut(s) 16, 86, 196, 316
SsiI CCGC 3 cut(s) 92, 363, 876
SspMI CTAG 2 cut(s) 756, 783
StyD4I CCNGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 510, 642, 905
TaiI ACGT 2 cut(s) 471, 622
TaqI TCGA 2 cut(s) 348, 553
TaqII GACCGA 1 cut(s) 164
TasI AATT 4 cut(s) 16, 86, 196, 316
TfiI GAWTC 6 cut(s) 31, 206, 292, 492, 683, 828
TscAI CASTG 2 cut(s) 748, 908
TseFI GTSAC 2 cut(s) 176, 308
Tsp45I GTSAC 2 cut(s) 176, 308
TspDTI ATGAA 5 cut(s) 92, 189, 266, 274, 575
TspGWI ACGGA 3 cut(s) 162, 327, 504
TspRI CASTG 2 cut(s) 748, 908
Van91I CCANNNNNTGG 1 cut(s) 27
VpaK11BI GGWCC 2 cut(s) 383, 458
XapI RAATTY 2 cut(s) 86, 316
XceI RCATGY 2 cut(s) 228, 890
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 2 cut(s) 756, 783
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.