Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0012171

Physical Location & Seq

Genomic Coordinates Reverse (-)

5397466 .. 5400666

Gene Structure 3201 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGACAGAAGCAGAAGCAGAAGCAGCTCAAATGGTGGCCGCCAAGAGAAAACCGACAGAAACAGAGGAATCCGATATCTTCGCGCCTTGCTCTTTCTCCAGTCTCGGACTTCACACAACATTATGCGAACAACTCGAAGAGAGGTTGGGCTTCAAAGCTCCGACTCTGGTCCAAGCTCAAGCCATTCCGGTAATCCTCTCCGGCCGCCACGTGCTCGTCAATGCGGCCACCGGCACGGGGAAGACGGTGGCGTATTTGGCTCCGGTCATCCACCACCTGCACTTGTACCAGCCTCGGATTCAGCGCTCTGACGGAACATTCGCGCTGGTTCTTGTGCCCACGAGGGAGCTCTGCTTGCAGGTTCACGAGATTCTGCACAAGGTGCTGCACAGGTTTCATTGGATTGTTCCGGGTTATGTAATGGGGGGTGAAAACAGGTCTAAGGAGAAGGCCAGGCTGCGAAAAGGCATATCTATTCTTGTTGCAACTCCTGGACGGCTCTTGGATCACCTGAAGAATACGTCTTCTTTCGTGCACACGAATTTGCGCTGGATTATCTTCGATGAAGCAGATAGAATTTTGGAATTGGGATTTGGAAAAGAAATAGAGGAAATACTAGATCTTTTAGGCTCAAGGCAACATGGCTCGGCCGCTAAGAATGGCGCTGTTACAAGTAAATATGAGTTTCCAAGGCAGAATTTGCTATTATCAGCTACCTTAAATGAAAAAGTCAATCACCTTGCAAAAATGAGTTTGGAGAAACCTGTTATGATTGGCATGCCTGAGAAGAAGAGGCAGTCTATTGCATTGCATGAGCATTTAGGATCCGATCTCACGGATGATGATGACACACAAACAATGAAGACAACAGGCTCTATTAGTGAAGATTACAGACTTCCAGCTCAATTGACACAGAAATATGTAAAAGTACCATGTAATGCGCGGCTTGCTGTACTTCTTTCGATTCTAAAGTATCTTTTTGAGCGACAAACTTCCGAAAAGATTGTGCTATTCTTTTCAACATGTGATGCAGTGGATTTTCACTACTCCTTGTTAAGTGAGTTCCAATTCAAACCTTCTTCAGGACCAGATGCAGAACTTAAGCAGAGGTTTGTTAATTGTAAGACCTTCCGGTTACATGGGAATATGGAGCAGGAGGACCGAAGAACTGCGTTTGAGACCTTCAAAAGAGAAAAATCAGCTCTTCTTTTGTCCACAGATGTTTGTGCTAGAGGTTTGGATTTCCCGAAAGTAAAATGTATTATACAATATGATTCTCCAGGCGAGGCTACTGAATATGTTCATAGGGTTGGAAGAACCGCTCGGTTGGGTGAACGAGGAGATTCATTGTTGTTTCTACAGCCAATTGAAATGGATTATTTGAAAGAGTTGGAGAAACATGGTGTTTCTCTAACAGAGTTTCCCCTCCTCAAAATATTAGATAGTTTTCCACTATATGGTCATAAGCATGTCAGGAAATTTGTTTCCATTGACGCACATCCCTGGGTTATGTCTTTGCAAAAGGCGCTTGAAGCATTTACCGATAAGCCGGATATGAAGACACTGGCCAAAAACACATTTTGCTCGTGGGTTCGTGCATACACTGCCCACCGTGGTGAGCTGAAAAGAATATTTATGGTGAAGAAACTTCACTTGGGACATGTTGCGAAAAGCTTTGCATTGAAAGACCAGCCTTCGTTGGTCGGTAAGTCATTCCACAATCAAGAAAAAAAGAGGAAGAGGGACGAAAAGCATAAGGGATCATCGAGAAAGAGGAAGGTTTCGAGGAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

599

Amino Acids

67.91

Weight (kDa)

9.38

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	56 - 245	2.6e-41	DEAD/DEAH box helicase
ResIII	PF04851	58 - 204	1.3e-09	Type III restriction enzyme, res subunit
Helicase_C	PF00271	321 - 444	4.1e-24	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	517 - 566	3.6e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40700
fragaria_vesca	FvH4_1g09140 FvH4_1g09140 FvH4_1g09140
malus_domestica	MD02G1096800.v1.1
prunus_persica	Prupe.7G194100_v2.0.a1
pyrus_communis	pycom02g07680 pycom15g19610
rosa_chinensis	RchiOBHm_Chr2g0095531
rosa_laevigata	RLG00000016576
rosa_multiflora	Rmu_co8308315.1_g000001 Rmu_co8333539.1_g000001
rosa_roxburghii	Rroxscaffold_2G00146220
rosa_rugosa	Rorug02G0052200
rosa_samantha	Rh2AG100000 Rh2BG101400 Rh2CG103600 Rh2DG100900
rosa_wichuraiana	Rw2G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	285
Acc36I	ACCTGC	2 cut(s)	285, 349
AccB7I	CCANNNNNTGG	1 cut(s)	1457
AccBSI	CCGCTC	1 cut(s)	1322
AccII	CGCG	3 cut(s)	83, 323, 943
AciI	CCGC	6 cut(s)	39, 205, 224, 651, 943, 1320
AclWI	GGATC	4 cut(s)	513, 819, 832, 1768
AcoI	YGGCCR	5 cut(s)	36, 202, 225, 648, 1566
AcsI	RAATTY	4 cut(s)	541, 576, 697, 1478
AcuI	CTGAAG	2 cut(s)	533, 1065
AcvI	CACGTG	1 cut(s)	211
AdeI	CACNNNGTG	1 cut(s)	382
AfaI	GTAC	3 cut(s)	287, 930, 954
AfeI	AGCGCT	1 cut(s)	305
AfiI	CCNNNNNNNGG	3 cut(s)	237, 1082, 1457
AflII	CTTAAG	1 cut(s)	1100
AflIII	ACRYGT	2 cut(s)	1022, 1660
AgsI	TTSAA	8 cut(s)	154, 1020, 1072, 1186, 1370, 1384, 1532, 1684
AjnI	CCWGG	4 cut(s)	452, 490, 1279, 1502
AjuI	GAANNNNNNNTTGG	4 cut(s)	576, 608, 1637, 1669
AleI	CACNNNNGTG	1 cut(s)	1614
AluBI	AGCT	9 cut(s)	26, 158, 176, 349, 713, 902, 1202, 1621, 1674
AluI	AGCT	9 cut(s)	26, 158, 176, 349, 713, 902, 1202, 1621, 1674
Alw21I	GWGCWC	3 cut(s)	216, 351, 537
Alw26I	GTCTC	2 cut(s)	107, 1172
Alw44I	GTGCAC	1 cut(s)	533
AlwI	GGATC	4 cut(s)	513, 819, 832, 1768
Aor51HI	AGCGCT	1 cut(s)	305
AoxI	GGCC	6 cut(s)	36, 202, 225, 450, 648, 1566
ApaLI	GTGCAC	1 cut(s)	533
ApeKI	GCWGC	3 cut(s)	23, 385, 457
ApoI	RAATTY	4 cut(s)	541, 576, 697, 1478
ArsI	GACNNNNNNTTYG	2 cut(s)	302, 334
Asp700I	GAANNNNTTC	1 cut(s)	1299
AspLEI	GCGC	7 cut(s)	85, 306, 325, 549, 665, 943, 1528
AspS9I	GGNCC	3 cut(s)	169, 1085, 1159
AsuC2I	CCSGG	1 cut(s)	411
AsuHPI	GGTGA	6 cut(s)	440, 500, 728, 1343, 1628, 1651
AvaII	GGWCC	3 cut(s)	169, 1085, 1159
BaeGI	GKGCMC	2 cut(s)	339, 537
BalI	TGGCCA	1 cut(s)	1568
BamHI	GGATCC	1 cut(s)	824
BanII	GRGCYC	1 cut(s)	351
BauI	CACGAG	3 cut(s)	340, 365, 1585
BbrPI	CACGTG	1 cut(s)	211
BbsI	GAAGAC	4 cut(s)	248, 516, 869, 1565
Bbv12I	GWGCWC	3 cut(s)	216, 351, 537
BbvI	GCAGC	3 cut(s)	35, 372, 444
BceAI	ACGGC	1 cut(s)	512
BciT130I	CCWGG	4 cut(s)	454, 492, 1281, 1504
BcnI	CCSGG	1 cut(s)	411
BcoDI	GTCTC	2 cut(s)	107, 1172
BfaI	CTAG	2 cut(s)	617, 1230
BfmI	CTRYAG	1 cut(s)	1358
BfoI	RGCGCY	3 cut(s)	307, 666, 1529
BfrI	CTTAAG	1 cut(s)	1100
BfuAI	ACCTGC	2 cut(s)	285, 349
BglII	AGATCT	1 cut(s)	619
BisI	GCNGC	8 cut(s)	24, 39, 205, 225, 386, 458, 651, 944
BlsI	GCNGC	8 cut(s)	25, 40, 206, 226, 387, 459, 652, 945
Bme1390I	CCNGG	5 cut(s)	411, 454, 492, 1281, 1504
Bme18I	GGWCC	3 cut(s)	169, 1085, 1159
BmgT120I	GGNCC	3 cut(s)	169, 1085, 1159
BmiI	GGNNCC	2 cut(s)	261, 826
BmrFI	CCNGG	5 cut(s)	411, 454, 492, 1281, 1504
BmsI	GCATC	2 cut(s)	1018, 1081
BoxI	GACNNNNGTC	1 cut(s)	167
BpiI	GAAGAC	4 cut(s)	248, 516, 869, 1565
BpmI	CTGGAG	2 cut(s)	82, 1263
BpuEI	CTTGAG	2 cut(s)	162, 616
BpuMI	CCSGG	1 cut(s)	411
BsaAI	YACGTR	1 cut(s)	211
BsaI	GGTCTC	1 cut(s)	1172
BsaJI	CCNNGG	5 cut(s)	293, 689, 1502, 1503, 1612
BsaWI	WCCGGW	3 cut(s)	187, 262, 1131
Bsc4I	CCNNNNNNNGG	3 cut(s)	237, 1082, 1457
Bse118I	RCCGGY	1 cut(s)	230
Bse1I	ACTGG	2 cut(s)	99, 1569
Bse3DI	GCAATG	1 cut(s)	806
BseBI	CCWGG	4 cut(s)	454, 492, 1281, 1504
BseDI	CCNNGG	5 cut(s)	293, 689, 1502, 1503, 1612
BseGI	GGATG	3 cut(s)	267, 844, 1498
BseLI	CCNNNNNNNGG	3 cut(s)	237, 1082, 1457
BseMI	GCAATG	1 cut(s)	806
BseMII	CTCAG	1 cut(s)	774
BseNI	ACTGG	2 cut(s)	99, 1569
BseRI	GAGGAG	2 cut(s)	1353, 1418
BseSI	GKGCMC	2 cut(s)	339, 537
BseX3I	CGGCCG	2 cut(s)	202, 648
BseXI	GCAGC	3 cut(s)	35, 372, 444
BsgI	GTGCAG	3 cut(s)	263, 359, 371
Bsh1236I	CGCG	3 cut(s)	83, 323, 943
Bsh1285I	CGRYCG	2 cut(s)	205, 651
BshFI	GGCC	6 cut(s)	38, 204, 227, 452, 650, 1568
BsiEI	CGRYCG	2 cut(s)	205, 651
BsiHKAI	GWGCWC	3 cut(s)	216, 351, 537
BsiSI	CCGG	7 cut(s)	188, 201, 231, 263, 410, 1132, 1550
BslFI	GGGAC	2 cut(s)	1671, 1757
BslI	CCNNNNNNNGG	3 cut(s)	237, 1082, 1457
BsmAI	GTCTC	2 cut(s)	107, 1172
BsmFI	GGGAC	2 cut(s)	1671, 1757
BsnI	GGCC	6 cut(s)	38, 204, 227, 452, 650, 1568
Bso31I	GGTCTC	1 cut(s)	1172
Bsp1286I	GDGCHC	4 cut(s)	216, 339, 351, 537
Bsp143I	GATC	5 cut(s)	505, 619, 824, 829, 1760
BspACI	CCGC	6 cut(s)	39, 205, 224, 651, 943, 1320
BspANI	GGCC	6 cut(s)	38, 204, 227, 452, 650, 1568
BspCNI	CTCAG	1 cut(s)	775
BspFNI	CGCG	3 cut(s)	83, 323, 943
BspLI	GGNNCC	2 cut(s)	261, 826
BspMI	ACCTGC	2 cut(s)	285, 349
BspPI	GGATC	4 cut(s)	513, 819, 832, 1768
BspQI	GCTCTTC	1 cut(s)	1209
BspTI	CTTAAG	1 cut(s)	1100
BspTNI	GGTCTC	1 cut(s)	1172
BsrBI	CCGCTC	1 cut(s)	1322
BsrDI	GCAATG	1 cut(s)	806
BsrFI	RCCGGY	1 cut(s)	230
BsrI	ACTGG	2 cut(s)	99, 1569
BssAI	RCCGGY	1 cut(s)	230
BssECI	CCNNGG	5 cut(s)	293, 689, 1502, 1503, 1612
BssMI	GATC	5 cut(s)	505, 619, 824, 829, 1760
BssSI	CACGAG	3 cut(s)	340, 365, 1585
BssT1I	CCWWGG	1 cut(s)	689
Bst2BI	CACGAG	3 cut(s)	340, 365, 1585
Bst2UI	CCWGG	4 cut(s)	454, 492, 1281, 1504
Bst4CI	ACNGT	2 cut(s)	247, 1613
Bst6I	CTCTTC	4 cut(s)	132, 785, 1209, 1733
BstAFI	CTTAAG	1 cut(s)	1100
BstAPI	GCANNNNNTGC	3 cut(s)	382, 700, 1604
BstBAI	YACGTR	1 cut(s)	211
BstC8I	GCNNGC	3 cut(s)	356, 779, 948
BstDEI	CTNAG	3 cut(s)	441, 654, 783
BstDSI	CCRYGG	1 cut(s)	1612
BstENI	CCTNNNNNAGG	1 cut(s)	1080
BstF5I	GGATG	3 cut(s)	267, 844, 1498
BstFNI	CGCG	3 cut(s)	83, 323, 943
BstH2I	RGCGCY	3 cut(s)	307, 666, 1529
BstHHI	GCGC	7 cut(s)	85, 306, 325, 549, 665, 943, 1528
BstKTI	GATC	5 cut(s)	508, 622, 827, 832, 1763
BstMAI	GTCTC	2 cut(s)	107, 1172
BstMBI	GATC	5 cut(s)	505, 619, 824, 829, 1760
BstMCI	CGRYCG	2 cut(s)	205, 651
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 257, 355, 382, 700, 947, 1525, 1532, 1604
BstNI	CCWGG	4 cut(s)	454, 492, 1281, 1504
BstNSI	RCATGY	4 cut(s)	781, 1026, 1472, 1664
BstPAI	GACNNNNGTC	1 cut(s)	167
BstSCI	CCNGG	5 cut(s)	409, 452, 490, 1279, 1502
BstSFI	CTRYAG	1 cut(s)	1358
BstSLI	GKGCMC	2 cut(s)	339, 537
BstUI	CGCG	3 cut(s)	83, 323, 943
BstV1I	GCAGC	3 cut(s)	35, 372, 444
BstV2I	GAAGAC	4 cut(s)	248, 516, 869, 1565
BstX2I	RGATCY	2 cut(s)	619, 824
BstYI	RGATCY	2 cut(s)	619, 824
BstZI	CGGCCG	2 cut(s)	202, 648
BsuRI	GGCC	6 cut(s)	38, 204, 227, 452, 650, 1568
BtgI	CCRYGG	1 cut(s)	1612
BtsCI	GGATG	3 cut(s)	267, 844, 1498
BtsI	GCAGTG	2 cut(s)	1038, 1602
BtsIMutI	CAGTG	3 cut(s)	1038, 1562, 1602
BveI	ACCTGC	2 cut(s)	285, 349
Cac8I	GCNNGC	3 cut(s)	356, 779, 948
CfoI	GCGC	7 cut(s)	85, 306, 325, 549, 665, 943, 1528
Cfr10I	RCCGGY	1 cut(s)	230
Cfr13I	GGNCC	3 cut(s)	169, 1085, 1159
CseI	GACGC	1 cut(s)	1502
Csp6I	GTAC	3 cut(s)	286, 929, 953
CviQI	GTAC	3 cut(s)	286, 929, 953
DdeI	CTNAG	3 cut(s)	441, 654, 783
DpnI	GATC	5 cut(s)	507, 621, 826, 831, 1762
DpnII	GATC	5 cut(s)	505, 619, 824, 829, 1760
DraIII	CACNNNGTG	1 cut(s)	382
EaeI	YGGCCR	5 cut(s)	36, 202, 225, 648, 1566
EagI	CGGCCG	2 cut(s)	202, 648
Eam1104I	CTCTTC	4 cut(s)	132, 785, 1209, 1733
EarI	CTCTTC	4 cut(s)	132, 785, 1209, 1733
Ecl136II	GAGCTC	1 cut(s)	349
EclXI	CGGCCG	2 cut(s)	202, 648
Eco130I	CCWWGG	1 cut(s)	689
Eco24I	GRGCYC	1 cut(s)	351
Eco31I	GGTCTC	1 cut(s)	1172
Eco32I	GATATC	1 cut(s)	76
Eco47I	GGWCC	3 cut(s)	169, 1085, 1159
Eco47III	AGCGCT	1 cut(s)	305
Eco52I	CGGCCG	2 cut(s)	202, 648
Eco53kI	GAGCTC	1 cut(s)	349
Eco57I	CTGAAG	2 cut(s)	533, 1065
Eco72I	CACGTG	1 cut(s)	211
EcoICRI	GAGCTC	1 cut(s)	349
EcoNI	CCTNNNNNAGG	1 cut(s)	1080
EcoRII	CCWGG	4 cut(s)	452, 490, 1279, 1502
EcoRV	GATATC	1 cut(s)	76
EcoT14I	CCWWGG	1 cut(s)	689
EcoT38I	GRGCYC	1 cut(s)	351
ErhI	CCWWGG	1 cut(s)	689
FaqI	GGGAC	2 cut(s)	1671, 1757
Fnu4HI	GCNGC	8 cut(s)	24, 39, 205, 225, 386, 458, 651, 944
FokI	GGATG	3 cut(s)	254, 851, 1485
FriOI	GRGCYC	1 cut(s)	351
Fsp4HI	GCNGC	8 cut(s)	24, 39, 205, 225, 386, 458, 651, 944
FspBI	CTAG	2 cut(s)	617, 1230
GlaI	GCGC	7 cut(s)	84, 305, 324, 548, 664, 942, 1527
GluI	GCNGC	8 cut(s)	24, 39, 205, 225, 386, 458, 651, 944
GsuI	CTGGAG	2 cut(s)	82, 1263
HaeII	RGCGCY	3 cut(s)	307, 666, 1529
HaeIII	GGCC	6 cut(s)	38, 204, 227, 452, 650, 1568
HapII	CCGG	7 cut(s)	188, 201, 231, 263, 410, 1132, 1550
HgaI	GACGC	1 cut(s)	1502
HhaI	GCGC	7 cut(s)	85, 306, 325, 549, 665, 943, 1528
Hin6I	GCGC	7 cut(s)	83, 304, 323, 547, 663, 941, 1526
HinP1I	GCGC	7 cut(s)	83, 304, 323, 547, 663, 941, 1526
HindIII	AAGCTT	1 cut(s)	1672
HinfI	GANTC	7 cut(s)	68, 163, 298, 370, 964, 1274, 1343
HpaII	CCGG	7 cut(s)	188, 201, 231, 263, 410, 1132, 1550
HphI	GGTGA	6 cut(s)	440, 500, 728, 1343, 1628, 1651
Hpy166II	GTNNAC	4 cut(s)	364, 535, 1215, 1334
Hpy188I	TCNGA	7 cut(s)	73, 107, 162, 297, 310, 829, 997
Hpy188III	TCNNGA	6 cut(s)	365, 1083, 1246, 1474, 1724, 1767
Hpy8I	GTNNAC	4 cut(s)	364, 535, 1215, 1334
HpyAV	CCTTC	6 cut(s)	442, 1086, 1138, 1192, 1704, 1771
HpyCH4III	ACNGT	2 cut(s)	247, 1613
HpyCH4IV	ACGT	2 cut(s)	210, 521
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 257, 355, 382, 700, 947, 1525, 1532, 1604
HpyF3I	CTNAG	3 cut(s)	441, 654, 783
HpySE526I	ACGT	2 cut(s)	210, 521
HspAI	GCGC	7 cut(s)	83, 304, 323, 547, 663, 941, 1526
Kzo9I	GATC	5 cut(s)	505, 619, 824, 829, 1760
LguI	GCTCTTC	1 cut(s)	1209
LmnI	GCTCC	4 cut(s)	163, 265, 346, 1150
Lsp1109I	GCAGC	3 cut(s)	35, 372, 444
LweI	GCATC	2 cut(s)	1018, 1081
MaeI	CTAG	2 cut(s)	617, 1230
MaeII	ACGT	2 cut(s)	210, 521
MaeIII	GTNAC	2 cut(s)	667, 1134
MalI	GATC	5 cut(s)	507, 621, 826, 831, 1762
MbiI	CCGCTC	1 cut(s)	1322
MboI	GATC	5 cut(s)	505, 619, 824, 829, 1760
MfeI	CAATTG	2 cut(s)	905, 1365
MflI	RGATCY	2 cut(s)	619, 824
MhlI	GDGCHC	4 cut(s)	216, 339, 351, 537
MlsI	TGGCCA	1 cut(s)	1568
MluCI	AATT	9 cut(s)	541, 576, 584, 697, 905, 1067, 1117, 1365, 1478
MluNI	TGGCCA	1 cut(s)	1568
MlyI	GAGTC	1 cut(s)	157
MmeI	TCCRAC	3 cut(s)	185, 1291, 1371
Mox20I	TGGCCA	1 cut(s)	1568
MroXI	GAANNNNTTC	1 cut(s)	1299
MscI	TGGCCA	1 cut(s)	1568
MseI	TTAA	4 cut(s)	719, 1055, 1101, 1116
MslI	CAYNNNNRTG	2 cut(s)	1467, 1614
Msp20I	TGGCCA	1 cut(s)	1568
MspCI	CTTAAG	1 cut(s)	1100
MspI	CCGG	7 cut(s)	188, 201, 231, 263, 410, 1132, 1550
MspR9I	CCNGG	5 cut(s)	411, 454, 492, 1281, 1504
MunI	CAATTG	2 cut(s)	905, 1365
MvaI	CCWGG	4 cut(s)	454, 492, 1281, 1504
MvnI	CGCG	3 cut(s)	83, 323, 943
MwoI	GCNNNNNNNGC	9 cut(s)	23, 257, 355, 382, 700, 947, 1525, 1532, 1604
NciI	CCSGG	1 cut(s)	411
NdeII	GATC	5 cut(s)	505, 619, 824, 829, 1760
NlaIV	GGNNCC	2 cut(s)	261, 826
NmeAIII	GCCGAG	1 cut(s)	626
NspI	RCATGY	4 cut(s)	781, 1026, 1472, 1664
OliI	CACNNNNGTG	1 cut(s)	1614
PaeI	GCATGC	1 cut(s)	781
PaqCI	CACCTGC	1 cut(s)	285
PasI	CCCWGGG	1 cut(s)	1503
PciI	ACATGT	2 cut(s)	1022, 1660
PciSI	GCTCTTC	1 cut(s)	1209
PdmI	GAANNNNTTC	1 cut(s)	1299
PfeI	GAWTC	6 cut(s)	68, 298, 370, 964, 1274, 1343
PflMI	CCANNNNNTGG	1 cut(s)	1457
PfoI	TCCNGGA	1 cut(s)	490
PkrI	GCNGC	8 cut(s)	25, 40, 206, 226, 387, 459, 652, 945
PleI	GAGTC	1 cut(s)	157
PmaCI	CACGTG	1 cut(s)	211
PmlI	CACGTG	1 cut(s)	211
PpsI	GAGTC	1 cut(s)	157
Ppu21I	YACGTR	1 cut(s)	211
PscI	ACATGT	2 cut(s)	1022, 1660
PshAI	GACNNNNGTC	1 cut(s)	167
Psp124BI	GAGCTC	1 cut(s)	351
Psp6I	CCWGG	4 cut(s)	452, 490, 1279, 1502
PspCI	CACGTG	1 cut(s)	211
PspGI	CCWGG	4 cut(s)	452, 490, 1279, 1502
PspN4I	GGNNCC	2 cut(s)	261, 826
PspPI	GGNCC	3 cut(s)	169, 1085, 1159
PsuI	RGATCY	2 cut(s)	619, 824
RsaI	GTAC	3 cut(s)	287, 930, 954
RsaNI	GTAC	3 cut(s)	286, 929, 953
RseI	CAYNNNNRTG	2 cut(s)	1467, 1614
SacI	GAGCTC	1 cut(s)	351
SapI	GCTCTTC	1 cut(s)	1209
SaqAI	TTAA	4 cut(s)	719, 1055, 1101, 1116
SatI	GCNGC	8 cut(s)	24, 39, 205, 225, 386, 458, 651, 944
Sau3AI	GATC	5 cut(s)	505, 619, 824, 829, 1760
Sau96I	GGNCC	3 cut(s)	169, 1085, 1159
SchI	GAGTC	1 cut(s)	157
ScrFI	CCNGG	5 cut(s)	411, 454, 492, 1281, 1504
SduI	GDGCHC	4 cut(s)	216, 339, 351, 537
SfaNI	GCATC	2 cut(s)	1018, 1081
SfcI	CTRYAG	1 cut(s)	1358
SinI	GGWCC	3 cut(s)	169, 1085, 1159
SmiMI	CAYNNNNRTG	2 cut(s)	1467, 1614
SmlI	CTYRAG	3 cut(s)	177, 631, 1100
SmoI	CTYRAG	3 cut(s)	177, 631, 1100
SphI	GCATGC	1 cut(s)	781
Sse9I	AATT	9 cut(s)	541, 576, 584, 697, 905, 1067, 1117, 1365, 1478
SsiI	CCGC	6 cut(s)	39, 205, 224, 651, 943, 1320
SspI	AATATT	2 cut(s)	1437, 1632
SspMI	CTAG	2 cut(s)	617, 1230
SstI	GAGCTC	1 cut(s)	351
StyD4I	CCNGG	5 cut(s)	409, 452, 490, 1279, 1502
StyI	CCWWGG	1 cut(s)	689
TaaI	ACNGT	2 cut(s)	247, 1613
TaiI	ACGT	2 cut(s)	213, 524
TaqI	TCGA	5 cut(s)	135, 561, 962, 1766, 1784
TaqII	GACCGA	1 cut(s)	1176
TasI	AATT	9 cut(s)	541, 576, 584, 697, 905, 1067, 1117, 1365, 1478
TatI	WGTACW	1 cut(s)	952
TauI	GCSGC	5 cut(s)	41, 207, 227, 653, 946
TfiI	GAWTC	6 cut(s)	68, 298, 370, 964, 1274, 1343
Tru1I	TTAA	4 cut(s)	719, 1055, 1101, 1116
Tru9I	TTAA	4 cut(s)	719, 1055, 1101, 1116
TscAI	CASTG	3 cut(s)	1038, 1569, 1609
TseI	GCWGC	3 cut(s)	23, 385, 457
TspDTI	ATGAA	7 cut(s)	386, 579, 738, 875, 1292, 1335, 1571
TspGWI	ACGGA	2 cut(s)	327, 851
TspRI	CASTG	3 cut(s)	1038, 1569, 1609
Van91I	CCANNNNNTGG	1 cut(s)	1457
Vha464I	CTTAAG	1 cut(s)	1100
VneI	GTGCAC	1 cut(s)	533
VpaK11BI	GGWCC	3 cut(s)	169, 1085, 1159
XagI	CCTNNNNNAGG	1 cut(s)	1080
XapI	RAATTY	4 cut(s)	541, 576, 697, 1478
XceI	RCATGY	4 cut(s)	781, 1026, 1472, 1664
XmnI	GAANNNNTTC	1 cut(s)	1299
XspI	CTAG	2 cut(s)	617, 1230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.