pycom02g11650

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009137
Physical Location & Seq
Reverse (-)
8222709 .. 8224380
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGATCCGATCGGTGATGGTGATAAACACGCAGGGGAAGCCTCGCCTTTCCAAGTTCTACGAATTTCAGGAACTTATACGCAGCGTCCATGGAGTGTTGTCCAGTAGAGGTGAGAATGTGAGCAATTTCGTGGATGCTGAGTCTATTTTCGGCCCGGATACTCGCCTTGTGTACAAGCACTTTGCAACTTTGTACATCGTCTTTGTATTCGATAGCTCTGAAAACGAGCTTGCCATGCTCGACCTCATACAAGTATTTGTGGAAACATTGGACAAATGCTTCAAAAATGTATGTGAGCTTGACGTGGTGATCAATTACAGCAAGATGCATACCGTATTGGATGAGATTATTTTTGGAGGTCAAGTGCTGGAAACAAGTTCAACTGAAGTTATGAAGGCCGTCGAAGAAATCTCAAGGTTAGAAGCAGCCTCAAATGCCATCTCACTGGTCCCAAAATCTGTTTCCGCCTGGCGGAGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

161

Amino Acids

18.09

Weight (kDa)

5.23

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 1 - 139 1.5e-40 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 465, 472
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 2 cut(s) 173, 194
AfiI CCNNNNNNNGG 1 cut(s) 471
AgsI TTSAA 2 cut(s) 283, 381
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 467
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 216, 229, 298
AluI AGCT 3 cut(s) 216, 229, 298
AoxI GGCC 2 cut(s) 151, 396
ApeKI GCWGC 2 cut(s) 81, 425
ApoI RAATTY 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 152, 448
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 4 cut(s) 25, 31, 122, 319
AvaII GGWCC 1 cut(s) 448
BbvI GCAGC 2 cut(s) 93, 437
BccI CCATC 2 cut(s) 10, 446
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 469
BciVI GTATCC 1 cut(s) 151
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 155
BfuI GTATCC 1 cut(s) 151
BisI GCNGC 2 cut(s) 82, 426
BlsI GCNGC 2 cut(s) 83, 427
Bme1390I CCNGG 2 cut(s) 155, 469
Bme18I GGWCC 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 2 cut(s) 152, 448
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 2 cut(s) 155, 469
BmsI GCATC 2 cut(s) 124, 315
BpuEI CTTGAG 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse1I ACTGG 2 cut(s) 102, 450
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 2 cut(s) 139, 346
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 102, 450
BseXI GCAGC 2 cut(s) 93, 437
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 153, 398
BsiEI CGRYCG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 155
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 471
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 2 cut(s) 153, 398
Bsp1407I TGTACA 2 cut(s) 171, 192
Bsp143I GATC 3 cut(s) 3, 8, 309
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 2 cut(s) 465, 472
BspANI GGCC 2 cut(s) 153, 398
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 450
BsrGI TGTACA 2 cut(s) 171, 192
BsrI ACTGG 2 cut(s) 102, 450
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 3 cut(s) 3, 8, 309
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 334
BstAUI TGTACA 2 cut(s) 171, 192
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 2 cut(s) 139, 346
BstKTI GATC 3 cut(s) 6, 11, 312
BstMBI GATC 3 cut(s) 3, 8, 309
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 37, 235, 434
BstNI CCWGG 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 153, 467
BstV1I GCAGC 2 cut(s) 93, 437
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuI GTATCC 1 cut(s) 151
BsuRI GGCC 2 cut(s) 153, 398
BtgI CCRYGG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 139, 346
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 231
Cfr13I GGNCC 2 cut(s) 152, 448
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 172, 193
CviAII CATG 2 cut(s) 89, 235
CviJI RGCY 7 cut(s) 40, 153, 216, 229, 298, 398, 428
CviKI_1 RGCY 7 cut(s) 40, 153, 216, 229, 298, 398, 428
CviQI GTAC 2 cut(s) 172, 193
DdeI CTNAG 1 cut(s) 138
DpnI GATC 3 cut(s) 5, 10, 311
DpnII GATC 3 cut(s) 3, 8, 309
EciI GGCGGA 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 2 cut(s) 92, 238
FaiI YATR 7 cut(s) 77, 90, 236, 248, 292, 330, 392
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 88, 234
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 82, 426
FokI GGATG 2 cut(s) 146, 353
Fsp4HI GCNGC 2 cut(s) 82, 426
GluI GCNGC 2 cut(s) 82, 426
HaeIII GGCC 2 cut(s) 153, 398
HapII CCGG 1 cut(s) 155
HgaI GACGC 1 cut(s) 73
Hin1II CATG 2 cut(s) 92, 238
HinfI GANTC 2 cut(s) 140, 475
HpaII CCGG 1 cut(s) 155
HphI GGTGA 4 cut(s) 25, 31, 122, 319
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 8, 220
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 185, 328
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 235, 434
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 2 cut(s) 92, 238
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 3 cut(s) 3, 8, 309
LpnPI CCDG 7 cut(s) 17, 53, 115, 168, 353, 431, 454
Lsp1109I GCAGC 2 cut(s) 93, 437
LweI GCATC 2 cut(s) 124, 315
MaeII ACGT 1 cut(s) 303
MalI GATC 3 cut(s) 5, 10, 311
MboI GATC 3 cut(s) 3, 8, 309
MboII GAAGA 1 cut(s) 416
MluCI AATT 3 cut(s) 62, 124, 313
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 5 cut(s) 51, 101, 254, 350, 439
Mph1103I ATGCAT 1 cut(s) 330
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 155, 469
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 37, 235, 434
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 88
NdeII GATC 3 cut(s) 3, 8, 309
NlaIII CATG 2 cut(s) 92, 238
NlaIV GGNNCC 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 330
PkrI GCNGC 2 cut(s) 83, 427
Ple19I CGATCG 1 cut(s) 11
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 2 cut(s) 152, 448
PvuI CGATCG 1 cut(s) 11
RsaI GTAC 2 cut(s) 173, 194
RsaNI GTAC 2 cut(s) 172, 193
SatI GCNGC 2 cut(s) 82, 426
Sau3AI GATC 3 cut(s) 3, 8, 309
Sau96I GGNCC 2 cut(s) 152, 448
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 155, 469
SetI ASST 8 cut(s) 112, 218, 231, 246, 300, 306, 361, 419
SfaNI GCATC 2 cut(s) 124, 315
SinI GGWCC 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 3 cut(s) 62, 124, 313
SsiI CCGC 2 cut(s) 465, 472
StyD4I CCNGG 2 cut(s) 153, 467
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 1 cut(s) 306
TaqI TCGA 4 cut(s) 210, 240, 402, 478
TasI AATT 3 cut(s) 62, 124, 313
TatI WGTACW 2 cut(s) 171, 192
TscAI CASTG 1 cut(s) 450
TseI GCWGC 2 cut(s) 81, 425
TspDTI ATGAA 1 cut(s) 407
TspRI CASTG 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.