pycom02g24080

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010072
Physical Location & Seq
Forward (+)
22194386 .. 22206094
11709 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4854 bp
ATGAAGGTGCCGTGCTGCTTGGTGTGCCAGACCCGCTACGACGAGGAGGAAAGAGTTCCGCTTTTGCTTCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCGAAAATGTTCTCTTCGTGTCCGGAAACTACCCTCGTCTGCCCCCGCTGCCGCCACGTCACCGTCGTCGGGAACTCCGTTCAGGCCCTCCGCAAGAACTTCGCTGTGCTCGCCTTGATTCACTCCAGCTCCAATGGTGTATCGTCTGCTGCCGCCTCCAATTTCGATTACGATTACACGGACGACGAGGATGGGGACGACGATGAGGATGATGATGGGGACCGACGGTGCGCTCGTGGGTCCCACACGTCGAGCTCGGGTGGGTGCGGACCAGCGATCGAGCTGGCGGTGCACCACGACTTGAAGTTGGTGCGGCGGATAGGTGAGGGGAGGCAGCCCGGGGTTCAAATGTGGACTGCTGTCATTGGAGGTGGAGGTGGAAGGTGCCGTCACCGGGTTGCGGTGAAGAAAGTGGCGGTGGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAGCTGGATAATTTGCGGTGGGCTTCGATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCTATGAAAAGTGAAGGCACCTTGTGTCTTGTTATGGATAAGTGTTATGGGTCGGTTCAGTCCGAGATGCAGCGCAATGAGGGCAGGCTGACTTTGGAGCAAATTCTAAGATACGGGGCTGACATTGCACGAGGAGTGGCTGAACTTCATGCGGCAGGTGTCATTTGTATGAATCTAAAACCGTCCAATCTTCTTTTGGATGAAAGTGGTCACGCGGTAGTTTCTGATTATGGAGTTGCTGCAATTCTGAAGAAACCTTCCTGCCGGAAAGCTCGATTGGAGTGTGACACCTCATCGATCCATTCTTGTATGGAGTGTACAATGCTTAGCCCCCACTATGCAGCTCCAGAGGCATGGGAGCCAGTGAAGAAGTTATTGAATCCATTTTGGGAGGATGCTATTGGTATTTCTGCTGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACTGGTTCCATTCCATGGGCTGGTTTAAGCACTGAGGAAATTTATCGGGCTGTAATCAAGACTCGGAAACTACCCCCACAATATGCAAGTGTAGTAGGTGTTGGGATACCTAGAGAATTGTGGAAAATGATTGGCGAGTGCCTGCAGTTCAAGGCATCGAAAAGACCATCTTTTAATTCAATGCTAGCCACTTTTCTTCGCCATTTGCAGGAGATACCACGCAGCCCTCCTGCAAGTCCTGATAATGTTTTAGCTAAATCCTCTGGATCAAATGTGATGGAACCATCTCCTGTATCCAAATCAGAAGTTTTTCAGGGTAACCCCACCCTTCTGCATAGACTTGTGTCTGAAGGGGATGTGCGCGGTGTTAGAGATCTGCTTCAAAAGGCCTCAGCAGAGAGTGATAACAGCACAATATTGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTCCATTTGGCCTGTAGACGCGGTAGTGCAGAACTTGTTAATTCTATTTTGGAGTATCAAGAGGCCAATGTGGATGTCTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCATTAGTAGCTGGATCCCCAGAATGCGTTCATGCTCTCATTAACAGCGGTGCTAATGTGAGATCTAGGTTGCGAGAAGGCTTTGGTCCCTCTGTTGCTCATGTCTGTGCCTATCATGGCCAACCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCAGATCCTAATGCGGTGGATGAGGAAGGTGAATCTGTATTGCATAGAGCTGTTGCGAAGAAATATACAGATTGTGCCCTTGTTGTTCTGGAAAATGGGGGCTCTAGGTCAATGTGTGTTTTGAATTCAGAAAAGTTTACACCATTGCACTTGTGCGTGGCAACATGGAATGTTGCTGTTGTTAGAAGGTGGGTGGAAGTTGCAACACCAGAAGAGATTGCTGATGCAATTGATAGACCAAGTCCAGTTGGCACTGCATTGTGTATGGCAGCTTCTCTAAAGAAAGATCATGAAATTGAGGGAAGAGAAATTGTGCAAATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAAGATTTGCAGCATGGACGGACAGCTTTGCATACATCTTCAATGGCCAATGAAGTTGAGTTGGTCAAGATTATACTTGATGCTGGAGTTGATGTGAACATTCGGAATGTACAAAATACAATTCCTCTTCACGTAGCACTGGCCAGAGGGGCAAAGTCATGTGTTGGACTGCTTCTATCAGCTGGAGCTAATTATAATTTGCAGGATGACGAAGGTGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTCGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTGCTGTTGAAGCTAGAAATCACAGTGGCAAGACTTTACGTGACTTTTTGGAGACACTTCCTCGGGAATGGATATCTGAAGATCTGATGGAGGCACTTGTAAATCGGGGAGTGCGTCTATTTGATGTAGGAGACTGGGTCAAATTTAAAAGAAGTATAACAGCTCCTATGTATGGCTGGCAAGATGCAAAACATAGAAGTGTTGGCTTTGTGCAAGGTGCCCCAGATAAGGACCATTTGATAGTATCATTTTGTTCTGGAGAGGCTCGTGTTTCGGTGAATGAAGTTGTAAAAGTTATTCCCTTAGACAGGGGTCAGCATGTCCAGCTGAAACCAGATGTCAAAGAACCCAGATTTGGGTGGCGTGGCCAGTCTCGCGATAGCATTGGTACTGTATTATGTGTCGATGATGATGGTATCCTACGTGTTGGCTTTCCCGGAGCCTCCAGAGGGTGGAAAGCTGATCCAGCAGAGATGGAGAGAGTTGAAGAATTCAAGGTTGGGGACTGGGTTCGAATTCGCCCCACTCTTACAACTGCAAAGCACGGATTAGGATCTGTTACAGCTGGTAGCATTGGTATTGTGTATTGCATAAGACCAGATAGTAGTCTGTTGCTGGAATTAAGCTATCTTCCAAGTCCATGGCATTGTGAACCAGAGGAGGTTGAGCTTGTAACTCCTTTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCTGTTGCAGAGCCTAGGTATGCATGGGGTGGTGAGACACACCATAGTGTTGGAAAAATAAGTGAGATAGAGAATGATGGGCTCCTGGTAATTGAAATACCAAATCGACCAATACCATGGCAGTCTGATCCTTCTGACATGGAGAAGGTGGAAGATTTCAAGGTAGGTGACTGGGTTAGAGTCAAAGCTTCTGTACCATCCCCCAGATATGGATGGGAGGATATCACAAGGAACAGTGTTGGGATAATTCATAGCTTGGAGGAAGATGGTGATATGGGTGTCGCCTTCTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAAAAGGTGCCCCCTTTTGAAGTGGGGCAAGAGATTCACATGATATCATCTGTTACTCAGCCAAGACTTGGATGGTCAAATGAAAGTCCAGCAACTGTTGGAAAAATTGTTAGGATTGATATGGATGGAGCTTTGAATACAAGAGTCACTGGCCGACAGAGCTTGTGGAAAGTTTCTCCTGGAGATGCGGAACGACTTTCTGGATTTGAAGTTGGTGACTGGGTACGTTCAAAACCAAGCCTGGGAACTCGACCAAGCTATGACTGGAACAGCGTTGGAAAGGAAAGTTTAGCTGTCGTGCACAGTGTTCAAGATACAGGTTACCTGGAGTTGGCTTGTTGTTTCCGTAAAGGCAGGTGGATAACTCATTACACAGATGTTGAAAAGGTCCCATGCTTTAAAATTGGGCAGTATGTCCGGTTCCGGACTGGGCTCGTTGAACCAAGATGGGGCTGGAGAGGGGCTCAACCTGATTCTCGAGGCGTCATAACTAGCGTTCATGCTGATGGGGAAGTGAGAGTGGCTTTCTCTGGTTTGCCGGGGTTGTGGAGAGGAGATCCTGCAGATCTTGAAATGGAGCAGATATTTGAAGTTGGAGAGTGGGTGAGATTAGAAGATCAAGCAGGTGCTTGGAAATCCATTGGACCGGGTAGTGTTGGAGTGGTGCAAGGATTGGGATACGATGCAGATACATGGGATGGAACCACATCCGTTGGATTCTGTGGGGAGCAAGAAAAATGGGTTGGGCCTACCTCTGCTCTTGAAAGGGTGAACAGGCTCATGGTTAGCCAGAAGGTTAGGGTTAAGCTGTCTGTCAAGCAGCCAAGATTTGGGTGGTCAGGCCACAGCCATGCAAGTCTTGGAACCATATCCACAATTGATGCTGATGGCAAGCTGAGAATATACACTCCAGCTGGTTCAAAACCTGTGATGATCGATCCATCAGAAGTGGAACTTGTGGAGGAAGAGGAGCTTCACATCGGTGACTGGGTTAGGGTTAAAACATCAGTTTCAACTCCAGCCCACCAGTGGGGAGAGGTGAATCGTTCAAGTGTTGGGCTTGTGCATCGGATGGAAAATGAGGAGCTCTGGGTGGCGTTTTGCTTCACAGAGAGGTTGTGGCTTTGCAAGGCATCGGAAATGGAACGGGTTAGGCCGTTTAAAGTGGGGGACAAAGTGAGGATTAGAGAAGGGCTGGTGACCCCACGGTGGGGTTGGGGAATGGAGACCCACGCGAGCAAAGGACAGGTAGTGGGGGTCGACGCAAATGGGAAGTTAAGGATTAAGTTCCGATGGAGAGAAGGGAAGCCGTGGATTGGAGACCCTGCAGATATTTCTCTTGATGAAAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1618

Amino Acids

178.07

Weight (kDa)

5.75

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 136 - 421 3.6e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 165 - 421 3.1e-29 Protein kinase domain
Ank_2 PF12796 470 - 570 2.7e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 582 - 661 4e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 582 - 626 1.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 702 - 786 3.5e-13 Ankyrin repeats (3 copies)
SH3_15 PF18346 859 - 977 1.5e-18 Mind bomb SH3 repeat domain
SH3_15 PF18346 1046 - 1103 6.2e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1109 - 1227 5.7e-12 Mind bomb SH3 repeat domain
SH3_15 PF18346 1235 - 1356 9.5e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1362 - 1483 1.4e-13 Mind bomb SH3 repeat domain
SH3_15 PF18346 1492 - 1610 7.8e-15 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2346
AarI CACCTGC 3 cut(s) 761, 3843, 4112
Acc36I ACCTGC 3 cut(s) 761, 3843, 4112
AccB1I GGYRCC 5 cut(s) 7, 486, 632, 2672, 3505
AccB7I CCANNNNNTGG 5 cut(s) 1089, 1094, 2907, 3566, 4328
AccI GTMKAC 2 cut(s) 1549, 4758
AccII CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
AccIII TCCGGA 2 cut(s) 124, 3921
AcoI YGGCCR 7 cut(s) 76, 1522, 1762, 2196, 2292, 2821, 3649
AcsI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2597, 2945, 2970
AcuI CTGAAG 5 cut(s) 53, 884, 1443, 2553, 3445
AcyI GRCGYC 1 cut(s) 3981
AdeI CACNNNGTG 1 cut(s) 639
AfaI GTAC 6 cut(s) 934, 1057, 2262, 2845, 3363, 3723
AflIII ACRYGT 2 cut(s) 348, 2878
AhdI GACNNNNNGTC 1 cut(s) 100
AjiI CACGTC 2 cut(s) 160, 351
AjnI CCWGG 4 cut(s) 3252, 3676, 3738, 3822
AjuI GAANNNNNNNTTGG 8 cut(s) 875, 907, 2793, 2825, 2937, 2969, 4224, 4256
AleI CACNNNNGTG 3 cut(s) 3213, 3494, 4479
Alw21I GWGCWC 7 cut(s) 213, 359, 396, 605, 1076, 3801, 4587
Alw26I GTCTC 8 cut(s) 524, 1500, 2501, 2580, 2832, 3197, 4718, 4812
Alw44I GTGCAC 4 cut(s) 392, 601, 1072, 3797
AlwNI CAGNNNCTG 1 cut(s) 3593
Ama87I CYCGRG 4 cut(s) 358, 440, 2517, 3975
Aor13HI TCCGGA 2 cut(s) 124, 3921
ApaLI GTGCAC 4 cut(s) 392, 601, 1072, 3797
ApoI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2597, 2945, 2970
Asp700I GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
AspA2I CCTAGG 1 cut(s) 3182
AspLEI GCGC 3 cut(s) 335, 690, 1437
AspS9I GGNCC 9 cut(s) 187, 322, 342, 371, 1730, 2686, 3886, 4142, 4244
AsuC2I CCSGG 6 cut(s) 441, 442, 497, 2892, 4038, 4146
AsuII TTCGAA 1 cut(s) 2968
AsuNHI GCTAGC 1 cut(s) 1258
AvaI CYCGRG 4 cut(s) 358, 440, 2517, 3975
AvaII GGWCC 7 cut(s) 322, 342, 371, 1730, 2686, 3886, 4142
AvrII CCTAGG 1 cut(s) 3182
BaeGI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 2677, 3510, 3801
BalI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
BamHI GGATCC 2 cut(s) 1627, 1658
BanI GGYRCC 5 cut(s) 7, 486, 632, 2672, 3505
BanII GRGCYC 6 cut(s) 359, 1904, 3252, 3933, 3964, 4587
BarI GAAGNNNNNNTAC 2 cut(s) 2056, 2088
BauI CACGAG 3 cut(s) 336, 744, 2721
Bbv12I GWGCWC 7 cut(s) 213, 359, 396, 605, 1076, 3801, 4587
BbvCI CCTCAGC 1 cut(s) 1465
BceAI ACGGC 3 cut(s) 474, 4639, 4792
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 4 cut(s) 3254, 3678, 3740, 3824
BciVI GTATCC 4 cut(s) 1173, 1378, 2882, 4169
BcnI CCSGG 6 cut(s) 441, 442, 497, 2892, 4038, 4146
BcoDI GTCTC 8 cut(s) 524, 1500, 2501, 2580, 2832, 3197, 4718, 4812
BfaI CTAG 7 cut(s) 1185, 1259, 1710, 1905, 2469, 3183, 3990
BfmI CTRYAG 4 cut(s) 1217, 1546, 4059, 4824
BfuAI ACCTGC 3 cut(s) 761, 3843, 4112
BfuI GTATCC 4 cut(s) 1173, 1378, 2882, 4169
BglI GCCNNNNNGGC 1 cut(s) 2300
BglII AGATCT 4 cut(s) 1447, 1706, 2536, 4063
BlnI CCTAGG 1 cut(s) 3182
BlpI GCTNAGC 1 cut(s) 941
Bme18I GGWCC 7 cut(s) 322, 342, 371, 1730, 2686, 3886, 4142
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 4 cut(s) 358, 440, 2517, 3975
BmgBI CACGTC 2 cut(s) 160, 351
BmgT120I GGNCC 9 cut(s) 187, 322, 342, 371, 1730, 2686, 3886, 4142, 4244
BmrI ACTGGG 6 cut(s) 2599, 2971, 3349, 3727, 3936, 4495
BmtI GCTAGC 1 cut(s) 1262
BmuI ACTGGG 6 cut(s) 2599, 2971, 3349, 3727, 3936, 4495
BoxI GACNNNNGTC 3 cut(s) 461, 1417, 2766
BplI GAGNNNNNCTC 2 cut(s) 3946, 3978
Bpu10I CCTNAGC 1 cut(s) 1465
Bpu1102I GCTNAGC 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 2968
BpuEI CTTGAG 1 cut(s) 2136
BpuMI CCSGG 6 cut(s) 441, 442, 497, 2892, 4038, 4146
Bsa29I ATCGAT 2 cut(s) 911, 4434
BsaAI YACGTR 4 cut(s) 2284, 2411, 2495, 2879
BsaBI GATNNNNATC 2 cut(s) 1626, 3007
BsaHI GRCGYC 1 cut(s) 3981
BsaI GGTCTC 2 cut(s) 4718, 4812
BsaWI WCCGGW 3 cut(s) 124, 3915, 3921
Bse3DI GCAATG 3 cut(s) 697, 738, 1943
Bse8I GATNNNNATC 2 cut(s) 1626, 3007
BseAI TCCGGA 2 cut(s) 124, 3921
BseBI CCWGG 4 cut(s) 3254, 3678, 3740, 3824
BseCI ATCGAT 2 cut(s) 911, 4434
BseJI GATNNNNATC 2 cut(s) 1626, 3007
BseMI GCAATG 3 cut(s) 697, 738, 1943
BseMII CTCAG 5 cut(s) 1020, 1098, 1479, 3569, 4385
BseRI GAGGAG 7 cut(s) 59, 542, 762, 3128, 4065, 4481, 4595
BseSI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 2677, 3510, 3801
BsgI GTGCAG 1 cut(s) 1581
Bsh1236I CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
Bsh1285I CGRYCG 1 cut(s) 381
BshNI GGYRCC 5 cut(s) 7, 486, 632, 2672, 3505
BshVI ATCGAT 2 cut(s) 911, 4434
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 7 cut(s) 213, 359, 396, 605, 1076, 3801, 4587
BsiHKCI CYCGRG 4 cut(s) 358, 440, 2517, 3975
BsiSI CCGG 9 cut(s) 125, 441, 496, 880, 2892, 3916, 3922, 4037, 4145
BslFI GGGAC 7 cut(s) 311, 328, 335, 1716, 2972, 3872, 4682
BsmAI GTCTC 8 cut(s) 524, 1500, 2501, 2580, 2832, 3197, 4718, 4812
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 7 cut(s) 311, 328, 335, 1716, 2972, 3872, 4682
BsmI GAATGC 1 cut(s) 1673
Bso31I GGTCTC 2 cut(s) 4718, 4812
BsoBI CYCGRG 4 cut(s) 358, 440, 2517, 3975
Bsp119I TTCGAA 1 cut(s) 2968
Bsp13I TCCGGA 2 cut(s) 124, 3921
Bsp1407I TGTACA 3 cut(s) 932, 1055, 2260
Bsp1720I GCTNAGC 1 cut(s) 941
Bsp19I CCATGG 4 cut(s) 79, 1088, 3095, 3284
Bsp68I TCGCGA 1 cut(s) 2832
BspCNI CTCAG 5 cut(s) 1021, 1099, 1478, 3568, 4386
BspDI ATCGAT 2 cut(s) 911, 4434
BspEI TCCGGA 2 cut(s) 124, 3921
BspFNI CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
BspHI TCATGA 1 cut(s) 2089
BspMAI CTGCAG 3 cut(s) 1221, 4063, 4828
BspMI ACCTGC 3 cut(s) 761, 3843, 4112
BspOI GCTAGC 1 cut(s) 1262
BspT104I TTCGAA 1 cut(s) 2968
BspT107I GGYRCC 5 cut(s) 7, 486, 632, 2672, 3505
BspTNI GGTCTC 2 cut(s) 4718, 4812
BsrDI GCAATG 3 cut(s) 697, 738, 1943
BsrGI TGTACA 3 cut(s) 932, 1055, 2260
BssNI GRCGYC 1 cut(s) 3981
BssSI CACGAG 3 cut(s) 336, 744, 2721
BssT1I CCWWGG 5 cut(s) 79, 1088, 3095, 3182, 3284
Bst2BI CACGAG 3 cut(s) 336, 744, 2721
Bst2UI CCWGG 4 cut(s) 3254, 3678, 3740, 3824
Bst4CI ACNGT 9 cut(s) 166, 330, 798, 2480, 2848, 3404, 3595, 3803, 4707
Bst6I CTCTTC 5 cut(s) 121, 2007, 2098, 2283, 4458
BstACI GRCGYC 1 cut(s) 3981
BstAPI GCANNNNNTGC 3 cut(s) 96, 2122, 2393
BstAUI TGTACA 3 cut(s) 932, 1055, 2260
BstBAI YACGTR 4 cut(s) 2284, 2411, 2495, 2879
BstBI TTCGAA 1 cut(s) 2968
BstDEI CTNAG 9 cut(s) 722, 941, 1029, 1107, 1465, 2441, 2758, 3555, 4394
BstDSI CCRYGG 6 cut(s) 79, 1088, 3095, 3284, 4703, 4808
BstEII GGTNACC 3 cut(s) 1391, 3818, 4696
BstENI CCTNNNNNAGG 1 cut(s) 2761
BstFNI CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
BstHHI GCGC 3 cut(s) 335, 690, 1437
BstMAI GTCTC 8 cut(s) 524, 1500, 2501, 2580, 2832, 3197, 4718, 4812
BstMCI CGRYCG 1 cut(s) 381
BstNI CCWGG 4 cut(s) 3254, 3678, 3740, 3824
BstNSI RCATGY 1 cut(s) 2777
BstPAI GACNNNNGTC 3 cut(s) 461, 1417, 2766
BstPI GGTNACC 3 cut(s) 1391, 3818, 4696
BstSFI CTRYAG 4 cut(s) 1217, 1546, 4059, 4824
BstSLI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 2677, 3510, 3801
BstUI CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
BstXI CCANNNNNNTGG 4 cut(s) 969, 3096, 3218, 3285
Bsu15I ATCGAT 2 cut(s) 911, 4434
BsuI GTATCC 4 cut(s) 1173, 1378, 2882, 4169
BsuTUI ATCGAT 2 cut(s) 911, 4434
BtgI CCRYGG 6 cut(s) 79, 1088, 3095, 3284, 4703, 4808
BtrI CACGTC 2 cut(s) 160, 351
BtsI GCAGTG 1 cut(s) 2052
BtuMI TCGCGA 1 cut(s) 2832
BveI ACCTGC 3 cut(s) 761, 3843, 4112
CaiI CAGNNNCTG 1 cut(s) 3593
CciI TCATGA 1 cut(s) 2089
CfoI GCGC 3 cut(s) 335, 690, 1437
Cfr13I GGNCC 9 cut(s) 187, 322, 342, 371, 1730, 2686, 3886, 4142, 4244
Cfr9I CCCGGG 1 cut(s) 440
ClaI ATCGAT 2 cut(s) 911, 4434
CseI GACGC 4 cut(s) 1560, 2558, 3970, 4769
Csp6I GTAC 6 cut(s) 933, 1056, 2261, 2844, 3362, 3722
CspCI CAANNNNNGTGG 2 cut(s) 4192, 4227
CviQI GTAC 6 cut(s) 933, 1056, 2261, 2844, 3362, 3722
DdeI CTNAG 9 cut(s) 722, 941, 1029, 1107, 1465, 2441, 2758, 3555, 4394
DraI TTTAAA 3 cut(s) 2602, 3898, 4660
DraIII CACNNNGTG 1 cut(s) 639
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 7 cut(s) 76, 1522, 1762, 2196, 2292, 2821, 3649
Eam1104I CTCTTC 5 cut(s) 121, 2007, 2098, 2283, 4458
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 5 cut(s) 121, 2007, 2098, 2283, 4458
EciI GGCGGA 2 cut(s) 433, 539
Ecl136II GAGCTC 2 cut(s) 357, 4585
Eco130I CCWWGG 5 cut(s) 79, 1088, 3095, 3182, 3284
Eco147I AGGCCT 1 cut(s) 1463
Eco24I GRGCYC 6 cut(s) 359, 1904, 3252, 3933, 3964, 4587
Eco31I GGTCTC 2 cut(s) 4718, 4812
Eco32I GATATC 3 cut(s) 2529, 3391, 3543
Eco47I GGWCC 7 cut(s) 322, 342, 371, 1730, 2686, 3886, 4142
Eco53kI GAGCTC 2 cut(s) 357, 4585
Eco57I CTGAAG 5 cut(s) 53, 884, 1443, 2553, 3445
Eco88I CYCGRG 4 cut(s) 358, 440, 2517, 3975
Eco91I GGTNACC 3 cut(s) 1391, 3818, 4696
EcoICRI GAGCTC 2 cut(s) 357, 4585
EcoNI CCTNNNNNAGG 1 cut(s) 2761
EcoO109I RGGNCCY 3 cut(s) 187, 342, 3886
EcoO65I GGTNACC 3 cut(s) 1391, 3818, 4696
EcoRI GAATTC 3 cut(s) 1924, 2945, 2970
EcoRII CCWGG 4 cut(s) 3252, 3676, 3738, 3822
EcoRV GATATC 3 cut(s) 2529, 3391, 3543
EcoT14I CCWWGG 5 cut(s) 79, 1088, 3095, 3182, 3284
EcoT22I ATGCAT 2 cut(s) 1042, 3193
EcoT38I GRGCYC 6 cut(s) 359, 1904, 3252, 3933, 3964, 4587
ErhI CCWWGG 5 cut(s) 79, 1088, 3095, 3182, 3284
Esp3I CGTCTC 1 cut(s) 524
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 1228, 1260, 4437, 4469, 4455, 4487
FaqI GGGAC 7 cut(s) 311, 328, 335, 1716, 2972, 3872, 4682
FauI CCCGC 2 cut(s) 41, 155
FblI GTMKAC 2 cut(s) 1549, 4758
FriOI GRGCYC 6 cut(s) 359, 1904, 3252, 3933, 3964, 4587
FspBI CTAG 7 cut(s) 1185, 1259, 1710, 1905, 2469, 3183, 3990
GlaI GCGC 3 cut(s) 334, 689, 1436
HapII CCGG 9 cut(s) 125, 441, 496, 880, 2892, 3916, 3922, 4037, 4145
HgaI GACGC 4 cut(s) 1560, 2558, 3970, 4769
HhaI GCGC 3 cut(s) 335, 690, 1437
Hin1I GRCGYC 1 cut(s) 3981
Hin6I GCGC 3 cut(s) 333, 688, 1435
HinP1I GCGC 3 cut(s) 333, 688, 1435
HincII GTYRAC 1 cut(s) 4759
HindII GTYRAC 1 cut(s) 4759
HindIII AAGCTT 1 cut(s) 3354
HpaII CCGG 9 cut(s) 125, 441, 496, 880, 2892, 3916, 3922, 4037, 4145
Hpy99I CGWCG 8 cut(s) 44, 170, 173, 290, 305, 330, 355, 4763
HpyCH4III ACNGT 9 cut(s) 166, 330, 798, 2480, 2848, 3404, 3595, 3803, 4707
HpyCH4IV ACGT 7 cut(s) 159, 350, 2283, 2410, 2494, 2878, 3724
HpyF3I CTNAG 9 cut(s) 722, 941, 1029, 1107, 1465, 2441, 2758, 3555, 4394
HpySE526I ACGT 7 cut(s) 159, 350, 2283, 2410, 2494, 2878, 3724
Hsp92I GRCGYC 1 cut(s) 3981
HspAI GCGC 3 cut(s) 333, 688, 1435
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 2 cut(s) 124, 3921
MaeI CTAG 7 cut(s) 1185, 1259, 1710, 1905, 2469, 3183, 3990
MaeII ACGT 7 cut(s) 159, 350, 2283, 2410, 2494, 2878, 3724
MfeI CAATTG 2 cut(s) 2028, 4374
MlsI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
MluNI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
MlyI GAGTC 4 cut(s) 1040, 1129, 3357, 3651
MmeI TCCRAC 8 cut(s) 2168, 2296, 3199, 3577, 3754, 4072, 4135, 4192
Mox20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
Mph1103I ATGCAT 2 cut(s) 1042, 3193
MroI TCCGGA 2 cut(s) 124, 3921
MroXI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
MscI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
MslI CAYNNNNRTG 8 cut(s) 782, 1749, 2652, 3213, 3494, 4002, 4347, 4479
Msp20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2823
MspA1I CMGCKG 7 cut(s) 150, 559, 1692, 2333, 2782, 3020, 4412
MspI CCGG 9 cut(s) 125, 441, 496, 880, 2892, 3916, 3922, 4037, 4145
MunI CAATTG 2 cut(s) 2028, 4374
Mva1269I GAATGC 1 cut(s) 1673
MvaI CCWGG 4 cut(s) 3254, 3678, 3740, 3824
MvnI CGCG 5 cut(s) 830, 1437, 1554, 2832, 4733
NciI CCSGG 6 cut(s) 441, 442, 497, 2892, 4038, 4146
NcoI CCATGG 4 cut(s) 79, 1088, 3095, 3284
NheI GCTAGC 1 cut(s) 1258
NruI TCGCGA 1 cut(s) 2832
NsiI ATGCAT 2 cut(s) 1042, 3193
NspI RCATGY 1 cut(s) 2777
NspV TTCGAA 1 cut(s) 2968
OliI CACNNNNGTG 3 cut(s) 3213, 3494, 4479
PaeR7I CTCGAG 1 cut(s) 3975
PagI TCATGA 1 cut(s) 2089
PaqCI CACCTGC 3 cut(s) 761, 3843, 4112
PceI AGGCCT 1 cut(s) 1463
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 177, 334, 2566
PctI GAATGC 1 cut(s) 1673
PdmI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
PfeI GAWTC 8 cut(s) 220, 787, 994, 1832, 3532, 3971, 4215, 4540
PflFI GACNNNGTC 2 cut(s) 2040, 2591
PflMI CCANNNNNTGG 5 cut(s) 1089, 1094, 2907, 3566, 4328
PfoI TCCNGGA 2 cut(s) 2890, 3676
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 4 cut(s) 1039, 1129, 3356, 3650
PpsI GAGTC 4 cut(s) 1039, 1129, 3356, 3650
Ppu21I YACGTR 4 cut(s) 2284, 2411, 2495, 2879
PpuMI RGGWCCY 2 cut(s) 342, 3886
PshAI GACNNNNGTC 3 cut(s) 461, 1417, 2766
PsiI TTATAA 1 cut(s) 2346
Psp124BI GAGCTC 2 cut(s) 359, 4587
Psp5II RGGWCCY 2 cut(s) 342, 3886
Psp6I CCWGG 4 cut(s) 3252, 3676, 3738, 3822
PspEI GGTNACC 3 cut(s) 1391, 3818, 4696
PspGI CCWGG 4 cut(s) 3252, 3676, 3738, 3822
PspPI GGNCC 9 cut(s) 187, 322, 342, 371, 1730, 2686, 3886, 4142, 4244
PspPPI RGGWCCY 2 cut(s) 342, 3886
PsrI GAACNNNNNNTAC 2 cut(s) 3902, 3934
PstI CTGCAG 3 cut(s) 1221, 4063, 4828
PstNI CAGNNNCTG 1 cut(s) 3593
PsyI GACNNNGTC 2 cut(s) 2040, 2591
PvuI CGATCG 1 cut(s) 381
PvuII CAGCTG 5 cut(s) 559, 2333, 2782, 3020, 4412
RruI TCGCGA 1 cut(s) 2832
RsaI GTAC 6 cut(s) 934, 1057, 2262, 2845, 3363, 3723
RsaNI GTAC 6 cut(s) 933, 1056, 2261, 2844, 3362, 3722
RseI CAYNNNNRTG 8 cut(s) 782, 1749, 2652, 3213, 3494, 4002, 4347, 4479
SacI GAGCTC 2 cut(s) 359, 4587
SalI GTCGAC 1 cut(s) 4757
Sau96I GGNCC 9 cut(s) 187, 322, 342, 371, 1730, 2686, 3886, 4142, 4244
SchI GAGTC 4 cut(s) 1040, 1129, 3357, 3651
SfcI CTRYAG 4 cut(s) 1217, 1546, 4059, 4824
Sfr274I CTCGAG 1 cut(s) 3975
SfuI TTCGAA 1 cut(s) 2968
SinI GGWCC 7 cut(s) 322, 342, 371, 1730, 2686, 3886, 4142
SlaI CTCGAG 1 cut(s) 3975
SmaI CCCGGG 1 cut(s) 442
SmiMI CAYNNNNRTG 8 cut(s) 782, 1749, 2652, 3213, 3494, 4002, 4347, 4479
SmlI CTYRAG 2 cut(s) 2151, 3975
SmoI CTYRAG 2 cut(s) 2151, 3975
SseBI AGGCCT 1 cut(s) 1463
SspI AATATT 1 cut(s) 1491
SspMI CTAG 7 cut(s) 1185, 1259, 1710, 1905, 2469, 3183, 3990
SstI GAGCTC 2 cut(s) 359, 4587
StuI AGGCCT 1 cut(s) 1463
StyI CCWWGG 5 cut(s) 79, 1088, 3095, 3182, 3284
TaaI ACNGT 9 cut(s) 166, 330, 798, 2480, 2848, 3404, 3595, 3803, 4707
TaiI ACGT 7 cut(s) 162, 353, 2286, 2413, 2497, 2881, 3727
TaqII GACCGA 1 cut(s) 339
TatI WGTACW 3 cut(s) 932, 1055, 2260
TauI GCSGC 5 cut(s) 78, 156, 257, 418, 770
TfiI GAWTC 8 cut(s) 220, 787, 994, 1832, 3532, 3971, 4215, 4540
TspGWI ACGGA 6 cut(s) 169, 296, 2185, 3015, 3833, 4198
TspMI CCCGGG 1 cut(s) 440
Tth111I GACNNNGTC 2 cut(s) 2040, 2591
Van91I CCANNNNNTGG 5 cut(s) 1089, 1094, 2907, 3566, 4328
VneI GTGCAC 4 cut(s) 392, 601, 1072, 3797
VpaK11BI GGWCC 7 cut(s) 322, 342, 371, 1730, 2686, 3886, 4142
XagI CCTNNNNNAGG 1 cut(s) 2761
XapI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2597, 2945, 2970
XceI RCATGY 1 cut(s) 2777
XcmI CCANNNNNNNNNTGG 8 cut(s) 808, 3567, 3759, 3948, 4329, 4355, 4524, 4710
XhoI CTCGAG 1 cut(s) 3975
XmaI CCCGGG 1 cut(s) 440
XmaJI CCTAGG 1 cut(s) 3182
XmiI GTMKAC 2 cut(s) 1549, 4758
XmnI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
XspI CTAG 7 cut(s) 1185, 1259, 1710, 1905, 2469, 3183, 3990
Zsp2I ATGCAT 2 cut(s) 1042, 3193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.