Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0008364

Physical Location & Seq

Genomic Coordinates Reverse (-)

22474445 .. 22476907

Gene Structure 2463 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g24350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGTCTTATGAAAAAAGGCTTGCTGTTGCCAGAAAAGCTGCCTCTCTCACTGCTCGCCACTGCCATAAGGTACAAAAGGTAGTTGTGGAGTCGGGCACTCGTTCAAACTCAGAAAAAGTTACTACCACTGCAGCAGCTGGTTATGATAGAGCTCCGGAAAGAGTACAAGAATCTGCAGATCATTGTGCTGTTGCCAGTGCTGCCGCTGATGGTGTCAATAGTGGCATTCGTAAAATGAAATTGGCAGAGAACTTAGCCTGTCTGGTAGAGGAATCCTCTCATATCGATGAGAGAAGACCCAAAGGCCGAATGGAAATCAAGAGGTGTCTTGAATTGCGCATAAAGAAGAGAGTGAAGGAACAATACATCAATGGGAAGTTTCGAGACCTAATGGTAAAAGTAATTGCCAATCCAGAAACTCTTCAAGATGCTTATGATTGTATTAGACTCAACTCAAATGTTGATATAGCATTAAGCGATGTGAAAAACTCTTTTGATTCTATGGCTGAAGAGATGCATCATGGGAGTTTTGATGTTAATGCCAATACCTTTTCTATCTCAAAAAGGGGTGTAGGGAATGAAATCTTAGTACTTCCTAATCTAAATCTGAAGGTCATTCAAGAAGCCATCAGAGTTGTTCTTGAAGTTGTTTACAAGCCCGACTTCTCTAAGATTTCACATGGTTATCGAAGTGGGAGGGGGCATTCCACAGCTCTGAAGTACATTAGCAAGGAGATTTCGAATCCTGATTGGTGGTTTACTGTGCTCTTAAATAAAAAGCTAGATGCTTGTATACTTGGCGAACTCTTAACAGCAATGGAGGGCAAGATAGTAGACCCCAGCCTCTTTGATATGATCAAAAGCATGTTTCATGCCAATGTGCTCAACTTGGAGTTTGGTGGTTTCCCGAAAGGTCATGGTCTTCCACAGGAGGGAATATTGTCCCCTATATTAATGAATATATATCTCGACCAATTTGACCGTGAATTTTACAGATTGTCGATGAAATATGAAGCTCTCAGTCTAGATTCTCAAAATGATCAAAAGTCCCAGTCTAAGCTGCGCAGCTGGTTTAGAAGACACCTGAAAGGTAATAATGATCTTGGCTGTGCGGGTGAAGAGAGTTGTAGTGCAAGGGTCCATTCTTGTCGCTTCATGGATGAGATATTTTTTTCGTTTTCTGGTTCTAAGGATGCTGCTCTTGAGTTCAAATCTGAGGTCCTAGATTACTTGCAGAAGTCTTTGCACTTGGAAGTCGATGATCAAACAGAATTATTGCCATGTCAAAAACTGCATGGGATTCGCTTTTTAGGCACTTTGGTTAGAAGAAATGTGATAGAAAGTCCTGCCACAAAAGCAGTCCACAAGTTGAAGGAAAAAGTTGCTTTGTTTGGTCTACAGAAGCAAGAGGCCTGGAATGTTGGGACGGTTCATATAGGGAAGAAATGGTTAGGTCATGGTTTGAAGAAGGTCAAGGAGTCAGAGATCAAGCATTTAGCGGACAGTAGTTCTGTTCTGAGCCAAATTTCCCATTTCCGTAAGTCTGGTATGGAAACTGATCATTGGTACAAGCACTTGTTGAAGATATGGATGCAAGACGTCAATGCCAAAGCAGAAGAGAGTGAGGAAGCTGTCCTGTCTAAGCATGTAGCAGAACCAGCTCTACCCGAAGAGCTAACAAACTCCTTTTACGAATTTCAGAGACAGGTGGAAAAGTATGTTTCTTCTGAGACCTCTTCTATTCTTGCCCTTTTGCCCAATACTGGTTCTTCCACTGAATCTGTCGTCATCACTGAGATAATTGCTCCTGTTAATGCCATTAAAAAGCGTCTCCAACGATATGGATTGACCACATCTGATGGTTATCCCCGTAGATCATCTTTGCTTGTTTTACAAGATAATGACCAAATCATCGACTGGTTTTCAGGTATTGTTCGCCGTTGGCTGAGATGGTATGCTGAGTGTGTAAACTTTAATGAGGTAAAGCTGTTGATTTCGGATATAGTTAGAAAATCTTGCATCCGAACTCTTGCAGCGAAGTATCGTGTGCATGAAAATGAGATAGAAAAACGTTTTGACACAGAACTAAGCAGGATTCCGTCTACCCAAGAAATAGAACAAGAGATGGTTGATGAGACATCAGATACTCAGGCATTTGAAAACGATGAGGCATTAATGTATGGAATTTCTTACAGTGGCTTGTGTTTACTCTCCCTTGCAAGAATGGTGAGCGAGTCAAGGCCTTGCAATTGTTTTGTGTTCGGGTGTATGGCTCCAGCACCGAGTGTTTATACCCTTCATGTCATGGAAAGGCAGAAGTTTCCGGGGTGGAAGACGGGGTTCTCCAGTTGCATTCATCCCAGCTTGAATAGGAGGAGAATTGGATTGTGCAAGCAGCATTTGAAGGATTTGTATCTTGGTCATGTATCACTTCAGTCCGTTGATTTCGGGGCGTGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000959 GO:0000963 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006109 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009507 GO:0009536 GO:0009791 GO:0009845 GO:0009889 GO:0009987 GO:0010467 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0019222 GO:0032501 GO:0032502 GO:0032881 GO:0032885 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048856 GO:0050789 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090351 GO:0090615 GO:0140053 GO:1900864 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Intron_maturas2 RVT_1

Protein Analysis

821

Amino Acids

93.02

Weight (kDa)

8.44

Isoelectric Point (pI)

51.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_1	PF00078	197 - 414	1.9e-07	Reverse transcriptase (RNA-dependent DNA polymerase)
Intron_maturas2	PF01348	600 - 700	1e-10	Type II intron maturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008364)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74350
fragaria_vesca	FvH4_7g02870
malus_domestica	MD02G1287300.v1.1
prunus_persica	Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1 Prupe.2G034000_v2.0.a1
pyrus_communis	pycom02g24350
rosa_chinensis	RchiOBHm_Chr1g0323821
rosa_laevigata	RLG00000030266
rosa_roxburghii	Rroxscaffold_4G00326400
rosa_rugosa	Rorug01G0039700
rosa_samantha	Rh1AG057100 Rh1BG048100 Rh1DG062200
rosa_wichuraiana	Rw1G004880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1602
Acc16I	TGCGCA	2 cut(s)	338, 1064
AccI	GTMKAC	4 cut(s)	793, 834, 1396, 2102
AccIII	TCCGGA	1 cut(s)	154
AciI	CCGC	3 cut(s)	204, 1112, 1499
AclI	AACGTT	1 cut(s)	2071
AcsI	RAATTY	4 cut(s)	986, 1524, 1694, 2184
AcuI	CTGAAG	4 cut(s)	528, 629, 737, 2417
AcyI	GRCGYC	1 cut(s)	1599
AdeI	CACNNNGTG	1 cut(s)	2285
AfaI	GTAC	5 cut(s)	72, 165, 591, 722, 1568
AfiI	CCNNNNNNNGG	2 cut(s)	932, 1763
AjnI	CCWGG	1 cut(s)	1412
Alw21I	GWGCWC	3 cut(s)	154, 768, 885
Alw26I	GTCTC	5 cut(s)	378, 1696, 1724, 1835, 2129
AlwNI	CAGNNNCTG	1 cut(s)	137
Aor13HI	TCCGGA	1 cut(s)	154
AoxI	GGCC	3 cut(s)	304, 1410, 2240
ApeKI	GCWGC	9 cut(s)	38, 131, 134, 200, 1060, 1065, 1196, 2033, 2395
ApoI	RAATTY	4 cut(s)	986, 1524, 1694, 2184
ArsI	GACNNNNNNTTYG	2 cut(s)	1203, 1235
AseI	ATTAAT	2 cut(s)	953, 2174
Asp700I	GAANNNNTTC	1 cut(s)	420
AspLEI	GCGC	2 cut(s)	339, 1065
AspS9I	GGNCC	2 cut(s)	1138, 1219
AsuC2I	CCSGG	1 cut(s)	2325
AsuHPI	GGTGA	2 cut(s)	1127, 2239
AsuII	TTCGAA	1 cut(s)	740
AvaII	GGWCC	2 cut(s)	1138, 1219
BaeGI	GKGCMC	1 cut(s)	98
BanII	GRGCYC	1 cut(s)	154
BarI	GAAGNNNNNNTAC	2 cut(s)	347, 379
BbsI	GAAGAC	4 cut(s)	301, 914, 1084, 2339
Bbv12I	GWGCWC	3 cut(s)	154, 768, 885
BbvI	GCAGC	9 cut(s)	25, 143, 146, 187, 1047, 1077, 1183, 2045, 2407
BccI	CCATC	5 cut(s)	203, 635, 1853, 1944, 2119
BceAI	ACGGC	1 cut(s)	1923
BcgI	CGANNNNNNTGC	2 cut(s)	1283, 1317
BciT130I	CCWGG	1 cut(s)	1414
BclI	TGATCA	4 cut(s)	855, 1039, 1261, 1558
BcnI	CCSGG	1 cut(s)	2325
BcoDI	GTCTC	5 cut(s)	378, 1696, 1724, 1835, 2129
BfaI	CTAG	3 cut(s)	782, 1025, 1223
BfmI	CTRYAG	3 cut(s)	129, 174, 1397
BmcAI	AGTACT	1 cut(s)	591
Bme1390I	CCNGG	2 cut(s)	1414, 2325
Bme18I	GGWCC	2 cut(s)	1138, 1219
BmgT120I	GGNCC	2 cut(s)	1138, 1219
BmiI	GGNNCC	2 cut(s)	1139, 2274
BmrFI	CCNGG	2 cut(s)	1414, 2325
BmrI	ACTGGG	1 cut(s)	1045
BmsI	GCATC	7 cut(s)	418, 504, 526, 775, 1183, 1581, 2028
BmuI	ACTGGG	1 cut(s)	1045
BpiI	GAAGAC	4 cut(s)	301, 914, 1084, 2339
BplI	GAGNNNNNCTC	2 cut(s)	260, 292
BpmI	CTGGAG	2 cut(s)	2259, 2329
Bpu14I	TTCGAA	1 cut(s)	740
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	1 cut(s)	2325
Bsa29I	ATCGAT	1 cut(s)	285
BsaBI	GATNNNNATC	2 cut(s)	1863, 2412
BsaHI	GRCGYC	1 cut(s)	1599
BsaI	GGTCTC	2 cut(s)	378, 1724
BsaJI	CCNNGG	1 cut(s)	2324
BsaWI	WCCGGW	1 cut(s)	154
Bsc4I	CCNNNNNNNGG	2 cut(s)	932, 1763
Bse1I	ACTGG	5 cut(s)	195, 1051, 1768, 1922, 2346
Bse3DI	GCAATG	1 cut(s)	822
Bse8I	GATNNNNATC	2 cut(s)	1863, 2412
BseAI	TCCGGA	1 cut(s)	154
BseBI	CCWGG	1 cut(s)	1414
BseCI	ATCGAT	1 cut(s)	285
BseDI	CCNNGG	1 cut(s)	2324
BseGI	GGATG	5 cut(s)	1165, 1198, 1596, 2019, 2356
BseJI	GATNNNNATC	2 cut(s)	1863, 2412
BseLI	CCNNNNNNNGG	2 cut(s)	932, 1763
BseMI	GCAATG	1 cut(s)	822
BseMII	CTCAG	9 cut(s)	123, 1033, 1206, 1508, 1719, 1785, 1937, 1950, 2162
BseNI	ACTGG	5 cut(s)	195, 1051, 1768, 1922, 2346
BseRI	GAGGAG	1 cut(s)	2389
BseSI	GKGCMC	1 cut(s)	98
BseXI	GCAGC	9 cut(s)	25, 143, 146, 187, 1047, 1077, 1183, 2045, 2407
BseYI	CCCAGC	2 cut(s)	839, 2360
BshFI	GGCC	3 cut(s)	306, 1412, 2242
BshVI	ATCGAT	1 cut(s)	285
BsiHKAI	GWGCWC	3 cut(s)	154, 768, 885
BsiSI	CCGG	2 cut(s)	155, 2324
BslFI	GGGAC	3 cut(s)	928, 1033, 1438
BslI	CCNNNNNNNGG	2 cut(s)	932, 1763
BsmAI	GTCTC	5 cut(s)	378, 1696, 1724, 1835, 2129
BsmBI	CGTCTC	1 cut(s)	1835
BsmFI	GGGAC	3 cut(s)	928, 1033, 1438
BsmI	GAATGC	3 cut(s)	225, 703, 2352
BsnI	GGCC	3 cut(s)	306, 1412, 2242
Bso31I	GGTCTC	2 cut(s)	378, 1724
Bsp119I	TTCGAA	1 cut(s)	740
Bsp1286I	GDGCHC	4 cut(s)	98, 154, 768, 885
Bsp13I	TCCGGA	1 cut(s)	154
Bsp143I	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
BspACI	CCGC	3 cut(s)	204, 1112, 1499
BspANI	GGCC	3 cut(s)	306, 1412, 2242
BspCNI	CTCAG	9 cut(s)	122, 1032, 1207, 1509, 1720, 1786, 1938, 1951, 2161
BspDI	ATCGAT	1 cut(s)	285
BspEI	TCCGGA	1 cut(s)	154
BspLI	GGNNCC	2 cut(s)	1139, 2274
BspMAI	CTGCAG	2 cut(s)	133, 178
BspQI	GCTCTTC	1 cut(s)	1665
BspT104I	TTCGAA	1 cut(s)	740
BspTNI	GGTCTC	2 cut(s)	378, 1724
BsrDI	GCAATG	1 cut(s)	822
BsrI	ACTGG	5 cut(s)	195, 1051, 1768, 1922, 2346
BssECI	CCNNGG	1 cut(s)	2324
BssMI	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
BssNAI	GTATAC	1 cut(s)	794
BssNI	GRCGYC	1 cut(s)	1599
Bst1107I	GTATAC	1 cut(s)	794
Bst2UI	CCWGG	1 cut(s)	1414
Bst4CI	ACNGT	5 cut(s)	763, 983, 1429, 1505, 2195
Bst6I	CTCTTC	7 cut(s)	341, 426, 504, 1113, 1611, 1665, 1741
BstACI	GRCGYC	1 cut(s)	1599
BstBI	TTCGAA	1 cut(s)	740
BstC8I	GCNNGC	3 cut(s)	21, 55, 2393
BstF5I	GGATG	5 cut(s)	1165, 1198, 1596, 2019, 2356
BstHHI	GCGC	2 cut(s)	339, 1065
BstKTI	GATC	8 cut(s)	181, 858, 1042, 1102, 1264, 1488, 1561, 1877
BstMAI	GTCTC	5 cut(s)	378, 1696, 1724, 1835, 2129
BstMBI	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
BstMWI	GCNNNNNNNGC	5 cut(s)	35, 200, 1311, 1355, 1658
BstNI	CCWGG	1 cut(s)	1414
BstNSI	RCATGY	2 cut(s)	868, 1649
BstSCI	CCNGG	2 cut(s)	1412, 2323
BstSFI	CTRYAG	3 cut(s)	129, 174, 1397
BstSLI	GKGCMC	1 cut(s)	98
BstV1I	GCAGC	9 cut(s)	25, 143, 146, 187, 1047, 1077, 1183, 2045, 2407
BstV2I	GAAGAC	4 cut(s)	301, 914, 1084, 2339
BstXI	CCANNNNNNTGG	1 cut(s)	1841
BstZ17I	GTATAC	1 cut(s)	794
Bsu15I	ATCGAT	1 cut(s)	285
BsuRI	GGCC	3 cut(s)	306, 1412, 2242
BsuTUI	ATCGAT	1 cut(s)	285
BtgZI	GCGATG	1 cut(s)	492
BtsCI	GGATG	5 cut(s)	1165, 1198, 1596, 2019, 2356
BtsI	GCAGTG	3 cut(s)	48, 58, 126
BtsIMutI	CAGTG	7 cut(s)	48, 58, 126, 202, 1773, 1791, 2200
Cac8I	GCNNGC	3 cut(s)	21, 55, 2393
CaiI	CAGNNNCTG	1 cut(s)	137
CfoI	GCGC	2 cut(s)	339, 1065
Cfr13I	GGNCC	2 cut(s)	1138, 1219
ClaI	ATCGAT	1 cut(s)	285
CseI	GACGC	1 cut(s)	1817
Csp6I	GTAC	5 cut(s)	71, 164, 590, 721, 1567
CviQI	GTAC	5 cut(s)	71, 164, 590, 721, 1567
DpnI	GATC	8 cut(s)	180, 857, 1041, 1101, 1263, 1487, 1560, 1876
DpnII	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
DraIII	CACNNNGTG	1 cut(s)	2285
Eam1104I	CTCTTC	7 cut(s)	341, 426, 504, 1113, 1611, 1665, 1741
EarI	CTCTTC	7 cut(s)	341, 426, 504, 1113, 1611, 1665, 1741
Ecl136II	GAGCTC	1 cut(s)	152
Eco147I	AGGCCT	2 cut(s)	1412, 2242
Eco24I	GRGCYC	1 cut(s)	154
Eco31I	GGTCTC	2 cut(s)	378, 1724
Eco47I	GGWCC	2 cut(s)	1138, 1219
Eco53kI	GAGCTC	1 cut(s)	152
Eco57I	CTGAAG	4 cut(s)	528, 629, 737, 2417
EcoICRI	GAGCTC	1 cut(s)	152
EcoO109I	RGGNCCY	1 cut(s)	1219
EcoRII	CCWGG	1 cut(s)	1412
EcoT22I	ATGCAT	1 cut(s)	519
EcoT38I	GRGCYC	1 cut(s)	154
Esp3I	CGTCTC	1 cut(s)	1835
FalI	AAGNNNNNCTT	2 cut(s)	647, 679
FaqI	GGGAC	3 cut(s)	928, 1033, 1438
FauI	CCCGC	1 cut(s)	1105
FbaI	TGATCA	4 cut(s)	855, 1039, 1261, 1558
FblI	GTMKAC	4 cut(s)	793, 834, 1396, 2102
FokI	GGATG	5 cut(s)	1172, 1205, 1603, 2006, 2343
FriOI	GRGCYC	1 cut(s)	154
FspBI	CTAG	3 cut(s)	782, 1025, 1223
FspI	TGCGCA	2 cut(s)	338, 1064
GlaI	GCGC	2 cut(s)	338, 1064
GsaI	CCCAGC	2 cut(s)	843, 2364
GsuI	CTGGAG	2 cut(s)	2259, 2329
HaeIII	GGCC	3 cut(s)	306, 1412, 2242
HapII	CCGG	2 cut(s)	155, 2324
HgaI	GACGC	1 cut(s)	1817
HhaI	GCGC	2 cut(s)	339, 1065
Hin1I	GRCGYC	1 cut(s)	1599
Hin6I	GCGC	2 cut(s)	337, 1063
HinP1I	GCGC	2 cut(s)	337, 1063
HpaII	CCGG	2 cut(s)	155, 2324
HphI	GGTGA	2 cut(s)	1127, 2239
Hpy166II	GTNNAC	9 cut(s)	652, 759, 794, 835, 1363, 1397, 1969, 2103, 2207
Hpy8I	GTNNAC	9 cut(s)	652, 759, 794, 835, 1363, 1397, 1969, 2103, 2207
HpyAV	CCTTC	6 cut(s)	349, 604, 1366, 1462, 2306, 2398
HpyCH4III	ACNGT	5 cut(s)	763, 983, 1429, 1505, 2195
HpyCH4IV	ACGT	2 cut(s)	1599, 2071
HpyF10VI	GCNNNNNNNGC	5 cut(s)	35, 200, 1311, 1355, 1658
HpySE526I	ACGT	2 cut(s)	1599, 2071
Hsp92I	GRCGYC	1 cut(s)	1599
HspAI	GCGC	2 cut(s)	337, 1063
Kpn2I	TCCGGA	1 cut(s)	154
Ksp22I	TGATCA	4 cut(s)	855, 1039, 1261, 1558
Kzo9I	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
LguI	GCTCTTC	1 cut(s)	1665
LmnI	GCTCC	3 cut(s)	157, 1810, 2278
Lsp1109I	GCAGC	9 cut(s)	25, 143, 146, 187, 1047, 1077, 1183, 2045, 2407
LweI	GCATC	7 cut(s)	418, 504, 526, 775, 1183, 1581, 2028
MaeI	CTAG	3 cut(s)	782, 1025, 1223
MaeII	ACGT	2 cut(s)	1599, 2071
MaeIII	GTNAC	1 cut(s)	118
MalI	GATC	8 cut(s)	180, 857, 1041, 1101, 1263, 1487, 1560, 1876
MboI	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
MfeI	CAATTG	1 cut(s)	2248
MhlI	GDGCHC	4 cut(s)	98, 154, 768, 885
MlyI	GAGTC	4 cut(s)	98, 441, 1487, 2243
MmeI	TCCRAC	1 cut(s)	1858
Mph1103I	ATGCAT	1 cut(s)	519
MroI	TCCGGA	1 cut(s)	154
MroXI	GAANNNNTTC	1 cut(s)	420
MseI	TTAA	9 cut(s)	473, 537, 770, 809, 953, 1812, 1821, 1974, 2174
MslI	CAYNNNNRTG	3 cut(s)	285, 876, 2055
MspA1I	CMGCKG	3 cut(s)	137, 206, 1068
MspI	CCGG	2 cut(s)	155, 2324
MspR9I	CCNGG	2 cut(s)	1414, 2325
MunI	CAATTG	1 cut(s)	2248
Mva1269I	GAATGC	3 cut(s)	225, 703, 2352
MvaI	CCWGG	1 cut(s)	1414
MwoI	GCNNNNNNNGC	5 cut(s)	35, 200, 1311, 1355, 1658
NciI	CCSGG	1 cut(s)	2325
NdeII	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
NlaIV	GGNNCC	2 cut(s)	1139, 2274
NsbI	TGCGCA	2 cut(s)	338, 1064
NsiI	ATGCAT	1 cut(s)	519
NspI	RCATGY	2 cut(s)	868, 1649
NspV	TTCGAA	1 cut(s)	740
PceI	AGGCCT	2 cut(s)	1412, 2242
PciSI	GCTCTTC	1 cut(s)	1665
PctI	GAATGC	3 cut(s)	225, 703, 2352
PdmI	GAANNNNTTC	1 cut(s)	420
PfeI	GAWTC	8 cut(s)	170, 272, 497, 742, 1028, 1300, 1778, 2095
PleI	GAGTC	4 cut(s)	97, 441, 1486, 2242
PpsI	GAGTC	4 cut(s)	97, 441, 1486, 2242
PpuMI	RGGWCCY	1 cut(s)	1219
PshBI	ATTAAT	2 cut(s)	953, 2174
Psp124BI	GAGCTC	1 cut(s)	154
Psp1406I	AACGTT	1 cut(s)	2071
Psp5II	RGGWCCY	1 cut(s)	1219
Psp6I	CCWGG	1 cut(s)	1412
PspFI	CCCAGC	2 cut(s)	839, 2360
PspGI	CCWGG	1 cut(s)	1412
PspN4I	GGNNCC	2 cut(s)	1139, 2274
PspPI	GGNCC	2 cut(s)	1138, 1219
PspPPI	RGGWCCY	1 cut(s)	1219
PsrI	GAACNNNNNNTAC	2 cut(s)	1647, 1679
PstI	CTGCAG	2 cut(s)	133, 178
PstNI	CAGNNNCTG	1 cut(s)	137
PvuII	CAGCTG	2 cut(s)	137, 1068
RsaI	GTAC	5 cut(s)	72, 165, 591, 722, 1568
RsaNI	GTAC	5 cut(s)	71, 164, 590, 721, 1567
RseI	CAYNNNNRTG	3 cut(s)	285, 876, 2055
SacI	GAGCTC	1 cut(s)	154
SapI	GCTCTTC	1 cut(s)	1665
SaqAI	TTAA	9 cut(s)	473, 537, 770, 809, 953, 1812, 1821, 1974, 2174
Sau3AI	GATC	8 cut(s)	178, 855, 1039, 1099, 1261, 1485, 1558, 1874
Sau96I	GGNCC	2 cut(s)	1138, 1219
ScaI	AGTACT	1 cut(s)	591
SchI	GAGTC	4 cut(s)	98, 441, 1487, 2243
ScrFI	CCNGG	2 cut(s)	1414, 2325
SduI	GDGCHC	4 cut(s)	98, 154, 768, 885
SfaNI	GCATC	7 cut(s)	418, 504, 526, 775, 1183, 1581, 2028
SfcI	CTRYAG	3 cut(s)	129, 174, 1397
SfuI	TTCGAA	1 cut(s)	740
SinI	GGWCC	2 cut(s)	1138, 1219
SmiMI	CAYNNNNRTG	3 cut(s)	285, 876, 2055
SmlI	CTYRAG	1 cut(s)	1202
SmoI	CTYRAG	1 cut(s)	1202
SseBI	AGGCCT	2 cut(s)	1412, 2242
SsiI	CCGC	3 cut(s)	204, 1112, 1499
SspI	AATATT	1 cut(s)	939
SspMI	CTAG	3 cut(s)	782, 1025, 1223
SstI	GAGCTC	1 cut(s)	154
StuI	AGGCCT	2 cut(s)	1412, 2242
StyD4I	CCNGG	2 cut(s)	1412, 2323
TaaI	ACNGT	5 cut(s)	763, 983, 1429, 1505, 2195
TaiI	ACGT	2 cut(s)	1602, 2074
TaqI	TCGA	8 cut(s)	285, 382, 688, 740, 969, 1001, 1257, 1914
TatI	WGTACW	3 cut(s)	163, 589, 720
TauI	GCSGC	1 cut(s)	206
TfiI	GAWTC	8 cut(s)	170, 272, 497, 742, 1028, 1300, 1778, 2095
Tru1I	TTAA	9 cut(s)	473, 537, 770, 809, 953, 1812, 1821, 1974, 2174
Tru9I	TTAA	9 cut(s)	473, 537, 770, 809, 953, 1812, 1821, 1974, 2174
TscAI	CASTG	7 cut(s)	55, 65, 133, 202, 1780, 1798, 2200
TseI	GCWGC	9 cut(s)	38, 131, 134, 200, 1060, 1065, 1196, 2033, 2395
TspGWI	ACGGA	3 cut(s)	1526, 2088, 2428
TspRI	CASTG	7 cut(s)	55, 65, 133, 202, 1780, 1798, 2200
VpaK11BI	GGWCC	2 cut(s)	1138, 1219
VspI	ATTAAT	2 cut(s)	953, 2174
XapI	RAATTY	4 cut(s)	986, 1524, 1694, 2184
XbaI	TCTAGA	1 cut(s)	1024
XceI	RCATGY	2 cut(s)	868, 1649
XcmI	CCANNNNNNNNNTGG	1 cut(s)	307
XmiI	GTMKAC	4 cut(s)	793, 834, 1396, 2102
XmnI	GAANNNNTTC	1 cut(s)	420
XspI	CTAG	3 cut(s)	782, 1025, 1223
ZraI	GACGTC	1 cut(s)	1600
ZrmI	AGTACT	1 cut(s)	591
Zsp2I	ATGCAT	1 cut(s)	519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.