pycom02g25260

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007095
Physical Location & Seq
Reverse (-)
23276078 .. 23280613
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGAAAACAATTAGGCCTTGTTTGGCACTTTTCTCTCTTCTCATTCTTTCTTGCTTCGTAGCTCAAATCCATGGAAGCAAGCAATCAGAAGCTCTTTCACGGCTTTACAAGTCCAAATTGAAGAACGCCGCCCCCATCGACAAGAGCCCTTTCGTGGCGATTCTTCACGCAAACACAACAAAAACTCATACTCAAGAGGGATTGAAGGAGAAGGATAGGATCGATAAGTTGCCTGGACAACCCAACGTGAAGTTCTCTCAGTATGGCGGGTACGTTACGGTGGATAAAAAAGCCGGTCGTGCATTATTTTACTACTTAGTTGAAGCTGAGAAAGCTAAGGATTCTTCACCTCTTCTTCTATGGCTCAATGGAGGACCTGGTTGTTCTTCTCTTGCCTATGGAGCAATGCTCGAGCTTGGACCGTTCCGAATCCACAGCGATGGAAAAACACTTTACAGAAACAGATTTTCATGGAATCATGCTGCCAATGTGTTGTTCCTTGAGTCTCCTGCTGGGGTGGGTTTTTCTTACTCAAATAGAACTTCTGATTACGATGCAAGCGGGGATAGAAACACAGCTGCAGATAATTATGTATTTTTGGTGAATTGGTTGGAGAGGTTTCCTGAATACAAGGGCAGGGAGTTTTATATTTCTGGGGAAAGCTATGCAGGGCATTATGTACCTCAACTTGCACATACCATTCTCAACCATAATAAGAGGGCAAACAAGACCATTATCAACCTCAAGGGCATTATAATTGGGAATGCAGTTATCAATGATGAAACTGATTCAAAAGGAACGTACGACTACCTTGCGACCCATGCTATGATTTCAGATAAAACTGCAAACAATATAAACAAGTACTGTAACTTTGCAGCTAATGTCACCACTCAACCTAAGGAGTGCAAAGATGCCACTGATGCTGCTGATAAAGATACCTACTACGTTGATATTTTTAACATCTATGCCCCTTTATGCTCGTCTACGAACCTCACTGCAAAACCCAAAAGGACTTCAATTTTCGAATTTGATCCGTGTAGTGAGTACTATGCATATGGTTATTTGAATCGGCCTGATGTTCAACAAGCTCTCCATGCCAATGTCACCAAACTGTCACACGACTGGGAACCATGTAGTGATATAATCACAAACTGGGGCGATAGCGCATCAACCGTACTACCTCTTTTACATGAGTTCCTGGATAATGGACTTCGAGTGTGGATTTTCAGTGGCGACATTGATGGGAGGGTATCAGTGACTTCAACAAAGTATTCTATTGACAAGATGAATCTTGCAGTAAAGACTGAGTGGCATGCTTGGTTCCTTAGTGGAGAGGTTGGTGGGTACACACAAGTGTACGAGGGAGGCCTAACTTTTGCTACAGTGAGAGAGGCAGGGCATCAAGTTCCAAGTTATCAGCCTGCAAGAGGACTTTCTCTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACTACAGGATATTACTAA

Protein Analysis

498

Amino Acids

55.44

Weight (kDa)

7.22

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 78 - 487 1.2e-143 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccI GTMKAC 1 cut(s) 983
AciI CCGC 3 cut(s) 129, 267, 561
AclWI GGATC 2 cut(s) 227, 1025
AcsI RAATTY 1 cut(s) 1025
AfaI GTAC 8 cut(s) 272, 681, 803, 863, 1046, 1176, 1346, 1358
AfiI CCNNNNNNNGG 2 cut(s) 154, 1427
AgsI TTSAA 8 cut(s) 121, 205, 323, 792, 1017, 1066, 1082, 1263
AjnI CCWGG 3 cut(s) 232, 376, 1197
AjuI GAANNNNNNNTTGG 4 cut(s) 236, 268, 479, 511
AleI CACNNNNGTG 1 cut(s) 1352
AluBI AGCT 9 cut(s) 62, 92, 326, 335, 415, 578, 663, 878, 1088
AluI AGCT 9 cut(s) 62, 92, 326, 335, 415, 578, 663, 878, 1088
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 2 cut(s) 227, 1025
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 3 cut(s) 14, 1070, 1366
ApeKI GCWGC 4 cut(s) 482, 578, 875, 923
ApoI RAATTY 1 cut(s) 1025
AspLEI GCGC 1 cut(s) 1166
AspS9I GGNCC 2 cut(s) 374, 419
AsuHPI GGTGA 4 cut(s) 339, 613, 877, 1096
AsuII TTCGAA 1 cut(s) 1023
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 2 cut(s) 374, 419
AxyI CCTNAGG 1 cut(s) 897
BanII GRGCYC 1 cut(s) 149
BbvI GCAGC 4 cut(s) 469, 565, 887, 910
BccI CCATC 4 cut(s) 143, 434, 1235, 1451
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 794, 828
BciT130I CCWGG 3 cut(s) 234, 378, 1199
BcoDI GTCTC 1 cut(s) 510
BfmI CTRYAG 3 cut(s) 579, 1380, 1479
BisI GCNGC 5 cut(s) 129, 483, 579, 876, 924
BlsI GCNGC 5 cut(s) 130, 484, 580, 877, 925
BmcAI AGTACT 2 cut(s) 863, 1046
Bme1390I CCNGG 3 cut(s) 234, 378, 1199
Bme18I GGWCC 2 cut(s) 374, 419
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 374, 419
BmiI GGNNCC 2 cut(s) 1128, 1322
BmrFI CCNGG 3 cut(s) 234, 378, 1199
BmrI ACTGGG 2 cut(s) 1132, 1162
BmsI GCATC 5 cut(s) 544, 901, 910, 1175, 1408
BmuI ACTGGG 2 cut(s) 1132, 1162
BplI GAGNNNNNCTC 2 cut(s) 393, 425
Bpu10I CCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 1023
BpuEI CTTGAG 3 cut(s) 177, 521, 728
Bsa29I ATCGAT 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 1074, 1104
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 1427
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 1127, 1157
Bse21I CCTNAGG 1 cut(s) 897
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 3 cut(s) 234, 378, 1199
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 70
BseLI CCNNNNNNNGG 2 cut(s) 154, 1427
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 272, 318, 1296
BseNI ACTGG 2 cut(s) 1127, 1157
BseXI GCAGC 4 cut(s) 469, 565, 887, 910
BseYI CCCAGC 1 cut(s) 512
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 3 cut(s) 16, 1072, 1368
BshVI ATCGAT 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 1 cut(s) 294
BsiWI CGTACG 1 cut(s) 801
BslI CCNNNNNNNGG 2 cut(s) 154, 1427
BsmAI GTCTC 1 cut(s) 510
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 3 cut(s) 16, 1072, 1368
BsoBI CYCGRG 1 cut(s) 410
Bsp119I TTCGAA 1 cut(s) 1023
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 2 cut(s) 219, 1030
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 3 cut(s) 129, 267, 561
BspANI GGCC 3 cut(s) 16, 1072, 1368
BspCNI CTCAG 3 cut(s) 271, 319, 1297
BspDI ATCGAT 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 1128, 1322
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 2 cut(s) 227, 1025
BspT104I TTCGAA 1 cut(s) 1023
BsrDI GCAATG 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 2 cut(s) 1127, 1157
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 2 cut(s) 219, 1030
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 3 cut(s) 234, 378, 1199
Bst4CI ACNGT 6 cut(s) 280, 423, 866, 1113, 1174, 1384
Bst6I CTCTTC 3 cut(s) 42, 357, 1473
BstBI TTCGAA 1 cut(s) 1023
BstC8I GCNNGC 4 cut(s) 80, 559, 1314, 1422
BstDEI CTNAG 7 cut(s) 258, 316, 327, 336, 897, 1305, 1325
BstDSI CCRYGG 1 cut(s) 70
BstENI CCTNNNNNAGG 1 cut(s) 1425
BstHHI GCGC 1 cut(s) 1166
BstKTI GATC 2 cut(s) 222, 1033
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 2 cut(s) 219, 1030
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 6 cut(s) 299, 332, 401, 821, 920, 1094
BstNI CCWGG 3 cut(s) 234, 378, 1199
BstNSI RCATGY 1 cut(s) 1316
BstSCI CCNGG 3 cut(s) 232, 376, 1197
BstSFI CTRYAG 3 cut(s) 579, 1380, 1479
BstV1I GCAGC 4 cut(s) 469, 565, 887, 910
BstXI CCANNNNNNTGG 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 897
BsuRI GGCC 3 cut(s) 16, 1072, 1368
BsuTUI ATCGAT 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 453
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 5 cut(s) 915, 993, 1234, 1260, 1389
Cac8I GCNNGC 4 cut(s) 80, 559, 1314, 1422
CfoI GCGC 1 cut(s) 1166
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 374, 419
ClaI ATCGAT 1 cut(s) 222
CsiI ACCWGGT 1 cut(s) 376
Csp6I GTAC 8 cut(s) 271, 680, 802, 862, 1045, 1175, 1345, 1357
CviAII CATG 8 cut(s) 71, 471, 479, 821, 1094, 1131, 1190, 1313
CviQI GTAC 8 cut(s) 271, 680, 802, 862, 1045, 1175, 1345, 1357
DdeI CTNAG 7 cut(s) 258, 316, 327, 336, 897, 1305, 1325
DpnI GATC 2 cut(s) 221, 1032
DpnII GATC 2 cut(s) 219, 1030
Eam1104I CTCTTC 3 cut(s) 42, 357, 1473
EarI CTCTTC 3 cut(s) 42, 357, 1473
Eco130I CCWWGG 1 cut(s) 70
Eco147I AGGCCT 2 cut(s) 16, 1368
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 374, 419
Eco81I CCTNAGG 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 1425
EcoO109I RGGNCCY 1 cut(s) 374
EcoRII CCWGG 3 cut(s) 232, 376, 1197
EcoT14I CCWWGG 1 cut(s) 70
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 8 cut(s) 74, 474, 482, 824, 1097, 1134, 1193, 1316
FatI CATG 8 cut(s) 70, 470, 478, 820, 1093, 1130, 1189, 1312
FauI CCCGC 2 cut(s) 260, 554
FauNDI CATATG 1 cut(s) 1054
FblI GTMKAC 1 cut(s) 983
Fnu4HI GCNGC 5 cut(s) 129, 483, 579, 876, 924
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 5 cut(s) 129, 483, 579, 876, 924
GlaI GCGC 1 cut(s) 1165
GluI GCNGC 5 cut(s) 129, 483, 579, 876, 924
GsaI CCCAGC 1 cut(s) 516
HaeIII GGCC 3 cut(s) 16, 1072, 1368
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 1166
Hin1II CATG 8 cut(s) 74, 474, 482, 824, 1097, 1134, 1193, 1316
Hin6I GCGC 1 cut(s) 1164
HinP1I GCGC 1 cut(s) 1164
HinfI GANTC 8 cut(s) 160, 341, 429, 475, 503, 788, 1066, 1288
HpaII CCGG 1 cut(s) 294
HphI GGTGA 4 cut(s) 339, 613, 877, 1096
Hpy166II GTNNAC 3 cut(s) 984, 1347, 1357
Hpy188I TCNGA 4 cut(s) 88, 428, 547, 835
Hpy188III TCNNGA 3 cut(s) 194, 623, 1472
Hpy8I GTNNAC 3 cut(s) 984, 1347, 1357
HpyAV CCTTC 2 cut(s) 199, 205
HpyCH4III ACNGT 6 cut(s) 280, 423, 866, 1113, 1174, 1384
HpyCH4IV ACGT 4 cut(s) 246, 273, 800, 945
HpyF10VI GCNNNNNNNGC 6 cut(s) 299, 332, 401, 821, 920, 1094
HpyF3I CTNAG 7 cut(s) 258, 316, 327, 336, 897, 1305, 1325
HpySE526I ACGT 4 cut(s) 246, 273, 800, 945
Hsp92II CATG 8 cut(s) 74, 474, 482, 824, 1097, 1134, 1193, 1316
HspAI GCGC 1 cut(s) 1164
Kzo9I GATC 2 cut(s) 219, 1030
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 4 cut(s) 469, 565, 887, 910
LweI GCATC 5 cut(s) 544, 901, 910, 1175, 1408
MabI ACCWGGT 1 cut(s) 376
MaeII ACGT 4 cut(s) 246, 273, 800, 945
MaeIII GTNAC 6 cut(s) 274, 866, 883, 1102, 1113, 1255
MalI GATC 2 cut(s) 221, 1032
MboI GATC 2 cut(s) 219, 1030
MboII GAAGA 8 cut(s) 29, 133, 155, 336, 344, 347, 378, 1460
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 7 cut(s) 9, 116, 586, 604, 756, 1017, 1025
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 591
Mph1103I ATGCAT 1 cut(s) 1054
MseI TTAA 1 cut(s) 957
MslI CAYNNNNRTG 3 cut(s) 438, 1098, 1352
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 3 cut(s) 234, 378, 1199
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 3 cut(s) 234, 378, 1199
MwoI GCNNNNNNNGC 6 cut(s) 299, 332, 401, 821, 920, 1094
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 1054
NdeII GATC 2 cut(s) 219, 1030
NlaIII CATG 8 cut(s) 74, 474, 482, 824, 1097, 1134, 1193, 1316
NlaIV GGNNCC 2 cut(s) 1128, 1322
NmuCI GTSAC 4 cut(s) 883, 1102, 1113, 1255
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 1 cut(s) 1316
NspV TTCGAA 1 cut(s) 1023
OliI CACNNNNGTG 1 cut(s) 1352
PaeI GCATGC 1 cut(s) 1316
PaeR7I CTCGAG 1 cut(s) 410
PceI AGGCCT 2 cut(s) 16, 1368
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 7 cut(s) 160, 341, 429, 475, 788, 1066, 1288
Pfl23II CGTACG 1 cut(s) 801
PfoI TCCNGGA 1 cut(s) 1197
PkrI GCNGC 5 cut(s) 130, 484, 580, 877, 925
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PpuMI RGGWCCY 1 cut(s) 374
PsiI TTATAA 1 cut(s) 755
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 3 cut(s) 232, 376, 1197
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 3 cut(s) 232, 376, 1197
PspLI CGTACG 1 cut(s) 801
PspN4I GGNNCC 2 cut(s) 1128, 1322
PspPI GGNCC 2 cut(s) 374, 419
PspPPI RGGWCCY 1 cut(s) 374
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 8 cut(s) 272, 681, 803, 863, 1046, 1176, 1346, 1358
RsaNI GTAC 8 cut(s) 271, 680, 802, 862, 1045, 1175, 1345, 1357
RseI CAYNNNNRTG 3 cut(s) 438, 1098, 1352
SaqAI TTAA 1 cut(s) 957
SatI GCNGC 5 cut(s) 129, 483, 579, 876, 924
Sau3AI GATC 2 cut(s) 219, 1030
Sau96I GGNCC 2 cut(s) 374, 419
ScaI AGTACT 2 cut(s) 863, 1046
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 3 cut(s) 234, 378, 1199
SduI GDGCHC 1 cut(s) 149
SexAI ACCWGGT 1 cut(s) 376
SfaNI GCATC 5 cut(s) 544, 901, 910, 1175, 1408
SfcI CTRYAG 3 cut(s) 579, 1380, 1479
Sfr274I CTCGAG 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 1023
SinI GGWCC 2 cut(s) 374, 419
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 3 cut(s) 438, 1098, 1352
SmlI CTYRAG 4 cut(s) 192, 410, 500, 743
SmoI CTYRAG 4 cut(s) 192, 410, 500, 743
SphI GCATGC 1 cut(s) 1316
Sse9I AATT 7 cut(s) 9, 116, 586, 604, 756, 1017, 1025
SseBI AGGCCT 2 cut(s) 16, 1368
SsiI CCGC 3 cut(s) 129, 267, 561
StuI AGGCCT 2 cut(s) 16, 1368
StyD4I CCNGG 3 cut(s) 232, 376, 1197
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 6 cut(s) 280, 423, 866, 1113, 1174, 1384
TaiI ACGT 4 cut(s) 249, 276, 803, 948
TaqI TCGA 5 cut(s) 138, 222, 411, 1023, 1213
TasI AATT 7 cut(s) 9, 116, 586, 604, 756, 1017, 1025
TatI WGTACW 2 cut(s) 861, 1044
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 7 cut(s) 160, 341, 429, 475, 788, 1066, 1288
Tru1I TTAA 1 cut(s) 957
Tru9I TTAA 1 cut(s) 957
TscAI CASTG 5 cut(s) 922, 1000, 1234, 1260, 1389
TseFI GTSAC 4 cut(s) 883, 1102, 1113, 1255
TseI GCWGC 4 cut(s) 482, 578, 875, 923
Tsp45I GTSAC 4 cut(s) 883, 1102, 1113, 1255
TspDTI ATGAA 4 cut(s) 17, 459, 795, 1301
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 5 cut(s) 922, 1000, 1234, 1260, 1389
VpaK11BI GGWCC 2 cut(s) 374, 419
XagI CCTNNNNNAGG 1 cut(s) 1425
XapI RAATTY 1 cut(s) 1025
XceI RCATGY 1 cut(s) 1316
XhoI CTCGAG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 983
ZrmI AGTACT 2 cut(s) 863, 1046
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.