pycom03g03140

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008473
Physical Location & Seq
Reverse (-)
2497894 .. 2500587
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGGCAGAAACGGAGGAACCCACAATACCCTTTAAGAATCTGAGTAGCAGAGAGTACCTGGGCCACAAGAAGAAGGTGCATTCTGTGGCTTGGAACTGCACGGGGACGAAGCTCGCGTCGGGTTCTGTTGATCGATCCGCTAGGGTTTGGCACATTGAGCCCCATGGACATGGTAAGGCTAAAGATATTGAATTGAAGGGGCACACAGATAGTGTGGATCAGCTGTGTTGGGACCCCAAGCATGCTGATTTGATTGCCACTGCCTCTGGGGACAAGACGGTTCGTCTTTGGGACGCTCGGAGTGGAAAATGTGCACAGCTAACAGAACTTAGCGGGGAGAACATCAATATCACCTATAAACCTGATGGGACACACATCGCCGTTGGTAATAGGGATGATGAACTAACAATTTTGGATGTTCGGAAGTTTAAGCCAATTCACAAACGAAAGTTCAATTATGAGGTTAATGAAATTGCTTGGAGCACAACAAGTGAAATTTTCTTCTTGACAACTGGAAACGGTACGGTTGAAGTACTAGCATACCCATCTCTTAAACCGGTCGACACTCTCATGGCTCATACAGCTGGTTGTTATTGCATTGCCATTGACCCACTTGGAAGATATTTTGCTGTTGGGAGTGCTGATTCCTTGGTCAGCCTGTGGGATATCTCAGAGATGCTCTGTGTGAAAACATTTCCAAAACTTGAATCACCTGTGAGGACAATAAGCTTCAACCACACTGGAGAGTATCTTGCGTCTGCCAGTGAAGACTTATTCATTGACATATCAAATGTTCAAACCGGGCGGACAGTACATCAGATTCCATGTAGGGCAGCCATGAACAGCGTTGAGTGGAATCCCAAATCCAATCTACTTGCATATGCTGGAGATGACAAGAACAAATATCAAACTGATGAAGGTGTTTTTAGGATATTTGGCTTCAAAAGTGCAACCTAA

Protein Analysis

319

Amino Acids

35.33

Weight (kDa)

6.31

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 16 - 143 8.1e-08 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 16 - 108 8.8e-15 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 16 - 170 1.5e-14 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 17 - 173 3.8e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 17 - 50 2.8e-07 WD domain, G-beta repeat
WD40_Prp19 PF24814 18 - 148 2.1e-18 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 19 - 135 6.3e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 19 - 138 6.9e-19 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 20 - 106 7.5e-13 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 21 - 145 3.4e-20 WDR5 beta-propeller domain
WDR55 PF24796 21 - 144 4.5e-09 WDR55
Beta-prop_THOC3 PF25174 22 - 295 3.4e-120 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 24 - 175 6.5e-10 Aladin seven-bladed propeller
Beta-prop_TEP1_2nd PF25047 26 - 147 3.3e-08 TEP-1 second beta-propeller
EIF3I PF24805 62 - 172 6.9e-06 EIF3I
WD40 PF00400 64 - 98 2.5e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 75 - 150 1.7e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st PF23389 75 - 224 3.8e-10 WDR19 first beta-propeller
WD40_WDHD1_1st PF24817 116 - 295 1.7e-20 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 116 - 295 6.3e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 116 - 293 4.5e-26 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 256 1.2e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 132 - 293 2.4e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 150 - 299 2.5e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 152 - 303 4.8e-18 Prp19 WD40 domain
WD40 PF00400 185 - 222 3.5e-07 WD domain, G-beta repeat
WD40_Gbeta PF25391 188 - 265 2.2e-08 G protein beta WD-40 repeat protein
EIF3I PF24805 189 - 254 2.7e-06 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 210 - 295 4.8e-06 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 116
AciI CCGC 3 cut(s) 138, 333, 805
AclWI GGATC 2 cut(s) 129, 225
AcsI RAATTY 1 cut(s) 495
AfaI GTAC 4 cut(s) 56, 523, 534, 813
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 8 cut(s) 191, 196, 454, 530, 707, 733, 797, 943
AhdI GACNNNNNGTC 1 cut(s) 282
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 5 cut(s) 112, 223, 319, 584, 729
AluI AGCT 5 cut(s) 112, 223, 319, 584, 729
Alw21I GWGCWC 2 cut(s) 316, 485
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 2 cut(s) 129, 225
AoxI GGCC 1 cut(s) 61
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 1 cut(s) 833
ApoI RAATTY 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AsiGI ACCGGT 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 61, 232
AsuC2I CCSGG 1 cut(s) 802
AsuHPI GGTGA 2 cut(s) 343, 702
AvaII GGWCC 1 cut(s) 232
BaeGI GKGCMC 2 cut(s) 204, 316
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 774
Bbv12I GWGCWC 2 cut(s) 316, 485
BbvI GCAGC 1 cut(s) 845
BccI CCATC 2 cut(s) 359, 553
BceAI ACGGC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 59
BcnI CCSGG 1 cut(s) 802
BfaI CTAG 2 cut(s) 141, 536
BisI GCNGC 1 cut(s) 834
BlsI GCNGC 1 cut(s) 835
BmcAI AGTACT 1 cut(s) 534
Bme1390I CCNGG 2 cut(s) 59, 802
Bme18I GGWCC 1 cut(s) 232
BmeRI GACNNNNNGTC 1 cut(s) 282
BmgT120I GGNCC 2 cut(s) 61, 232
BmiI GGNNCC 3 cut(s) 18, 233, 234
BmrFI CCNGG 2 cut(s) 59, 802
BmsI GCATC 1 cut(s) 666
BpiI GAAGAC 1 cut(s) 774
BpmI CTGGAG 2 cut(s) 762, 906
BpuMI CCSGG 1 cut(s) 802
Bsa29I ATCGAT 1 cut(s) 133
BsaJI CCNNGG 3 cut(s) 58, 163, 648
BsaWI WCCGGW 1 cut(s) 556
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 3 cut(s) 517, 745, 762
Bse3DI GCAATG 1 cut(s) 597
BseBI CCWGG 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 133
BseDI CCNNGG 3 cut(s) 58, 163, 648
BseGI GGATG 2 cut(s) 400, 421
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 2 cut(s) 32, 684
BseNI ACTGG 3 cut(s) 517, 745, 762
BseSI GKGCMC 2 cut(s) 204, 316
BseXI GCAGC 1 cut(s) 845
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 1 cut(s) 63
BshTI ACCGGT 1 cut(s) 556
BshVI ATCGAT 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 561
BsiHKAI GWGCWC 2 cut(s) 316, 485
BsiSI CCGG 2 cut(s) 557, 801
BslFI GGGAC 5 cut(s) 118, 245, 284, 305, 382
BsmFI GGGAC 5 cut(s) 118, 245, 284, 305, 382
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 1 cut(s) 63
Bsp1286I GDGCHC 4 cut(s) 162, 204, 316, 485
Bsp143I GATC 3 cut(s) 130, 134, 217
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 3 cut(s) 138, 333, 805
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 2 cut(s) 33, 683
BspDI ATCGAT 1 cut(s) 133
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 3 cut(s) 18, 233, 234
BspPI GGATC 2 cut(s) 129, 225
BsrDI GCAATG 1 cut(s) 597
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 3 cut(s) 517, 745, 762
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 58, 163, 648
BssMI GATC 3 cut(s) 130, 134, 217
BssT1I CCWWGG 2 cut(s) 163, 648
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 4 cut(s) 280, 521, 526, 811
BstC8I GCNNGC 2 cut(s) 114, 243
BstDEI CTNAG 3 cut(s) 41, 329, 670
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 2 cut(s) 400, 421
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 3 cut(s) 133, 137, 220
BstMBI GATC 3 cut(s) 130, 134, 217
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 2 cut(s) 157, 581
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 2 cut(s) 57, 800
BstSLI GKGCMC 2 cut(s) 204, 316
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 845
BstV2I GAAGAC 1 cut(s) 774
BstXI CCANNNNNNTGG 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 133
BsuRI GGCC 1 cut(s) 63
BsuTUI ATCGAT 1 cut(s) 133
BtgI CCRYGG 1 cut(s) 163
BtgZI GCGATG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 400, 421
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 258, 738, 769
Cac8I GCNNGC 2 cut(s) 114, 243
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 61, 232
ClaI ATCGAT 1 cut(s) 133
CseI GACGC 3 cut(s) 105, 302, 744
Csp6I GTAC 4 cut(s) 55, 522, 533, 812
CspAI ACCGGT 1 cut(s) 556
CviAII CATG 6 cut(s) 164, 170, 242, 571, 825, 838
CviQI GTAC 4 cut(s) 55, 522, 533, 812
DdeI CTNAG 3 cut(s) 41, 329, 670
DpnI GATC 3 cut(s) 132, 136, 219
DpnII GATC 3 cut(s) 130, 134, 217
DriI GACNNNNNGTC 1 cut(s) 282
Eam1105I GACNNNNNGTC 1 cut(s) 282
EciI GGCGGA 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 163, 648
Eco24I GRGCYC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 57
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 163, 648
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 2 cut(s) 163, 648
FaeI CATG 6 cut(s) 167, 173, 245, 574, 828, 841
FaqI GGGAC 5 cut(s) 118, 245, 284, 305, 382
FatI CATG 6 cut(s) 163, 169, 241, 570, 824, 837
FauI CCCGC 1 cut(s) 326
FauNDI CATATG 1 cut(s) 880
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 1 cut(s) 834
FokI GGATG 2 cut(s) 407, 428
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 834
FspBI CTAG 2 cut(s) 141, 536
GluI GCNGC 1 cut(s) 834
GsuI CTGGAG 2 cut(s) 762, 906
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 557, 801
HgaI GACGC 3 cut(s) 105, 302, 744
Hin1II CATG 6 cut(s) 167, 173, 245, 574, 828, 841
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 727
HinfI GANTC 5 cut(s) 37, 644, 707, 820, 856
HpaII CCGG 2 cut(s) 557, 801
HphI GGTGA 2 cut(s) 343, 702
Hpy166II GTNNAC 2 cut(s) 314, 562
Hpy188I TCNGA 5 cut(s) 42, 300, 423, 673, 819
Hpy188III TCNNGA 1 cut(s) 505
Hpy8I GTNNAC 2 cut(s) 314, 562
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 67, 190, 911
HpyCH4III ACNGT 4 cut(s) 280, 521, 526, 811
HpyCH4V TGCA 6 cut(s) 79, 99, 314, 597, 878, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 581
HpyF3I CTNAG 3 cut(s) 41, 329, 670
Hsp92II CATG 6 cut(s) 167, 173, 245, 574, 828, 841
KflI GGGWCCC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 130, 134, 217
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 1 cut(s) 845
LweI GCATC 1 cut(s) 666
MaeI CTAG 2 cut(s) 141, 536
MalI GATC 3 cut(s) 132, 136, 219
MboI GATC 3 cut(s) 130, 134, 217
MboII GAAGA 4 cut(s) 82, 493, 630, 779
MhlI GDGCHC 4 cut(s) 162, 204, 316, 485
MluCI AATT 6 cut(s) 191, 408, 435, 454, 471, 495
MnlI CCTC 4 cut(s) 7, 274, 454, 711
MseI TTAA 4 cut(s) 33, 429, 465, 552
MslI CAYNNNNRTG 2 cut(s) 168, 569
MspA1I CMGCKG 2 cut(s) 223, 584
MspI CCGG 2 cut(s) 557, 801
MspR9I CCNGG 2 cut(s) 59, 802
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 157, 581
NciI CCSGG 1 cut(s) 802
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 130, 134, 217
NlaIII CATG 6 cut(s) 167, 173, 245, 574, 828, 841
NlaIV GGNNCC 3 cut(s) 18, 233, 234
NspI RCATGY 1 cut(s) 245
PaeI GCATGC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PctI GAATGC 1 cut(s) 79
PfeI GAWTC 5 cut(s) 37, 644, 707, 820, 856
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 1 cut(s) 835
PpuMI RGGWCCY 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 3 cut(s) 18, 233, 234
PspPI GGNCC 2 cut(s) 61, 232
PspPPI RGGWCCY 1 cut(s) 232
PsrI GAACNNNNNNTAC 2 cut(s) 9, 41
PvuII CAGCTG 2 cut(s) 223, 584
RsaI GTAC 4 cut(s) 56, 523, 534, 813
RsaNI GTAC 4 cut(s) 55, 522, 533, 812
RseI CAYNNNNRTG 2 cut(s) 168, 569
SalI GTCGAC 1 cut(s) 560
SaqAI TTAA 4 cut(s) 33, 429, 465, 552
SatI GCNGC 1 cut(s) 834
Sau3AI GATC 3 cut(s) 130, 134, 217
Sau96I GGNCC 2 cut(s) 61, 232
ScaI AGTACT 1 cut(s) 534
ScrFI CCNGG 2 cut(s) 59, 802
SduI GDGCHC 4 cut(s) 162, 204, 316, 485
SfaNI GCATC 1 cut(s) 666
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 168, 569
SphI GCATGC 1 cut(s) 245
Sse9I AATT 6 cut(s) 191, 408, 435, 454, 471, 495
SsiI CCGC 3 cut(s) 138, 333, 805
SspMI CTAG 2 cut(s) 141, 536
StyD4I CCNGG 2 cut(s) 57, 800
StyI CCWWGG 2 cut(s) 163, 648
TaaI ACNGT 4 cut(s) 280, 521, 526, 811
TaqI TCGA 2 cut(s) 133, 561
TasI AATT 6 cut(s) 191, 408, 435, 454, 471, 495
TatI WGTACW 2 cut(s) 532, 811
TfiI GAWTC 5 cut(s) 37, 644, 707, 820, 856
Tru1I TTAA 4 cut(s) 33, 429, 465, 552
Tru9I TTAA 4 cut(s) 33, 429, 465, 552
TscAI CASTG 3 cut(s) 265, 745, 769
TseI GCWGC 1 cut(s) 833
TspDTI ATGAA 5 cut(s) 414, 483, 766, 854, 930
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 265, 745, 769
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 495
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 2 cut(s) 141, 536
ZrmI AGTACT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.