pycom03g03990

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005248
Physical Location & Seq
Forward (+)
3150095 .. 3154406
4312 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1281 bp
ATGGACGGCAGATATTGCCCCAACTATGCAGGAAGCTGCGATGGGTCACATGTCAAATCCTCAAACCCAAATTTTCCCATTTCCCAAACACGAACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCCAGCACTGGGATCTGTCTTCTTTCTCTTCAGTCTGCAAGAACAAAGCTCAAAGATTCTTGCGGAAGTATCAACTCGAAGTTTCAACGATATTAGCAAATGGCTAATTCTTTGGGTCTTTCACAGATCAAGCATAAGATATCTTGTTTTTACATTTCAGGTTGAGTCCAATAAGACTATGAAGAGGTTAGAATCAAGAAAGTCTCGTTCGTGGTGGTGGGATAGTCACATCAGTCCCAAGAACTCTAAGTGGCTTGCAGATAATCTCGAGGAGATGGATCGAAGCATCAAACGGATGTTGAAGCTGATCGAAGAGGATGGGGATTCATTTGCCAAAAAGGCTGAAATGTATTATCAAAAGAGGCCTGAATTGATTGCTCATGTTGAAGAGTTTTATCGCTTGTACCGGTCCCTGGCCGAACGTTATGATCATGTGACAGGAGAATTGCGGAAGAATGCACCCTCGGATCTCCAATCCCAGAGTTCATGCCTTTCTGACATTGGTTCAGAACTGCCATCTGCATTGCCCTCTCCTGATGTCCAACCCGGAAAGCTAGGTCGTCGTAAATCTGGCCCTCGTGCTGCTGGCTTTGATTTCTTCCTTGGCCCTGGTGGAAACAGCTCAGATCATTACCAGAAAGAGGGAGATGAATCATTTTCATTAGCCGATTATTCTGAGCCAGAATCTGATGATTCTTCAGTCAACAATTATTCAACTCCCTTGTCAAATGGTCTTGATCAAGGGCAGACGAGAAAGATAATTGAGTTGGAAATTGAGCTTCGTGAAGCGAAGGAGAAGCTCCGGATGCAGCAGGAAGAGAATGTGGACAGTTCATTCACGGGTGCAAAACCTGATCATACCGAAGAATTTCCTGCAAAAATTGCAGAATATGAGGAGGAGCTGACTAGCAGAAATGAAAAATTGCGGGAATCGGAAGAAGAGATTGCCAGGTTGAACATCAAGCTCAAAAGGTACGAGTCTTCAATAATGGTCTTAAGCTTGCACTTGAAGCAGAGCCTGCACAACATAATAATGGTCTTAAGCTTGCACTTGAACCAGAGCCTGCACAACATAATAATGGTCTTAAGCTTGCTCTTGAATTTTCAAAACCGAAAGAGACCAAGATACGTGAGGGTGCCAGAGACAGGGAGATAA

Protein Analysis

427

Amino Acids

48.96

Weight (kDa)

6.49

Isoelectric Point (pI)

70.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 112 - 185 1.2e-37 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1261
AccIII TCCGGA 1 cut(s) 927
AciI CCGC 3 cut(s) 188, 574, 1051
AclI AACGTT 1 cut(s) 547
AclWI GGATC 3 cut(s) 145, 411, 600
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 3 cut(s) 70, 992, 1225
AcuI CTGAAG 2 cut(s) 139, 807
AfaI GTAC 2 cut(s) 530, 1100
AfiI CCNNNNNNNGG 4 cut(s) 134, 538, 766, 1271
AflII CTTAAG 3 cut(s) 1120, 1165, 1210
AflIII ACRYGT 1 cut(s) 49
AgeI ACCGGT 1 cut(s) 531
AjnI CCWGG 3 cut(s) 537, 733, 1073
Alw26I GTCTC 3 cut(s) 333, 1237, 1262
AlwI GGATC 3 cut(s) 145, 411, 600
AlwNI CAGNNNCTG 3 cut(s) 812, 1144, 1189
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 927
AoxI GGCC 4 cut(s) 488, 540, 697, 730
ApeKI GCWGC 3 cut(s) 36, 707, 934
ApoI RAATTY 3 cut(s) 70, 992, 1225
AsiGI ACCGGT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 993
AspS9I GGNCC 3 cut(s) 534, 698, 731
AsuC2I CCSGG 1 cut(s) 672
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 534
BanI GGYRCC 1 cut(s) 1261
BauI CACGAG 1 cut(s) 702
BbsI GAAGAC 2 cut(s) 136, 1098
BbvI GCAGC 3 cut(s) 23, 694, 946
BccI CCATC 4 cut(s) 35, 394, 437, 649
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 3 cut(s) 539, 735, 1075
BclI TGATCA 3 cut(s) 553, 862, 979
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 3 cut(s) 333, 1237, 1262
BfaI CTAG 2 cut(s) 680, 1032
BfrI CTTAAG 3 cut(s) 1120, 1165, 1210
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 3 cut(s) 37, 708, 935
BlsI GCNGC 3 cut(s) 38, 709, 936
Bme1390I CCNGG 4 cut(s) 539, 672, 735, 1075
Bme18I GGWCC 1 cut(s) 534
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 534, 698, 731
BmiI GGNNCC 2 cut(s) 536, 1263
BmrFI CCNGG 4 cut(s) 539, 672, 735, 1075
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 420, 921
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 2 cut(s) 136, 1098
BpmI CTGGAG 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 1255
BsaI GGTCTC 1 cut(s) 1237
BsaJI CCNNGG 4 cut(s) 537, 588, 727, 733
BsaWI WCCGGW 2 cut(s) 531, 927
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 538, 766, 1271
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 647
BseAI TCCGGA 1 cut(s) 927
BseBI CCWGG 3 cut(s) 539, 735, 1075
BseDI CCNNGG 4 cut(s) 537, 588, 727, 733
BseGI GGATG 3 cut(s) 426, 448, 936
BseLI CCNNNNNNNGG 4 cut(s) 134, 538, 766, 1271
BseMI GCAATG 1 cut(s) 647
BseMII CTCAG 2 cut(s) 762, 792
BseNI ACTGG 1 cut(s) 138
BseRI GAGGAG 3 cut(s) 410, 1034, 1037
BseXI GCAGC 3 cut(s) 23, 694, 946
BsgI GTGCAG 2 cut(s) 1130, 1175
BshFI GGCC 4 cut(s) 490, 542, 699, 732
BshNI GGYRCC 1 cut(s) 1261
BshTI ACCGGT 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 3 cut(s) 532, 672, 928
BslFI GGGAC 2 cut(s) 345, 520
BslI CCNNNNNNNGG 4 cut(s) 134, 538, 766, 1271
BsmAI GTCTC 3 cut(s) 333, 1237, 1262
BsmFI GGGAC 2 cut(s) 345, 520
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 4 cut(s) 490, 542, 699, 732
Bso31I GGTCTC 1 cut(s) 1237
BsoBI CYCGRG 1 cut(s) 392
Bsp13I TCCGGA 1 cut(s) 927
Bsp143I GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
BspACI CCGC 3 cut(s) 188, 574, 1051
BspANI GGCC 4 cut(s) 490, 542, 699, 732
BspCNI CTCAG 2 cut(s) 761, 793
BspEI TCCGGA 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 536, 1263
BspPI GGATC 3 cut(s) 145, 411, 600
BspT107I GGYRCC 1 cut(s) 1261
BspTI CTTAAG 3 cut(s) 1120, 1165, 1210
BspTNI GGTCTC 1 cut(s) 1237
BsrDI GCAATG 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 4 cut(s) 537, 588, 727, 733
BssMI GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 727
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 3 cut(s) 539, 735, 1075
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 6 cut(s) 158, 302, 432, 507, 936, 1059
BstAFI CTTAAG 3 cut(s) 1120, 1165, 1210
BstAPI GCANNNNNTGC 3 cut(s) 15, 1007, 1144
BstBAI YACGTR 1 cut(s) 1255
BstC8I GCNNGC 7 cut(s) 381, 712, 1127, 1145, 1172, 1190, 1217
BstDEI CTNAG 3 cut(s) 372, 748, 801
BstF5I GGATG 3 cut(s) 426, 448, 936
BstKTI GATC 9 cut(s) 140, 254, 406, 435, 556, 595, 754, 865, 982
BstMAI GTCTC 3 cut(s) 333, 1237, 1262
BstMBI GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
BstMWI GCNNNNNNNGC 6 cut(s) 15, 464, 931, 1007, 1135, 1144
BstNI CCWGG 3 cut(s) 539, 735, 1075
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 4 cut(s) 537, 670, 733, 1073
BstV1I GCAGC 3 cut(s) 23, 694, 946
BstV2I GAAGAC 2 cut(s) 136, 1098
BstX2I RGATCY 2 cut(s) 137, 592
BstYI RGATCY 2 cut(s) 137, 592
BsuRI GGCC 4 cut(s) 490, 542, 699, 732
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 3 cut(s) 426, 448, 936
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 7 cut(s) 381, 712, 1127, 1145, 1172, 1190, 1217
CaiI CAGNNNCTG 3 cut(s) 812, 1144, 1189
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 3 cut(s) 534, 698, 731
Csp6I GTAC 2 cut(s) 529, 1099
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 4 cut(s) 50, 506, 557, 612
CviQI GTAC 2 cut(s) 529, 1099
DdeI CTNAG 3 cut(s) 372, 748, 801
DpnI GATC 9 cut(s) 139, 253, 405, 434, 555, 594, 753, 864, 981
DpnII GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 6 cut(s) 158, 302, 432, 507, 936, 1059
EarI CTCTTC 6 cut(s) 158, 302, 432, 507, 936, 1059
Eco130I CCWWGG 1 cut(s) 727
Eco147I AGGCCT 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 1237
Eco32I GATATC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 534
Eco57I CTGAAG 2 cut(s) 139, 807
Eco88I CYCGRG 1 cut(s) 392
EcoRII CCWGG 3 cut(s) 537, 733, 1073
EcoRV GATATC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 727
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 4 cut(s) 53, 509, 560, 615
FaqI GGGAC 2 cut(s) 345, 520
FatI CATG 4 cut(s) 49, 505, 556, 611
FauI CCCGC 1 cut(s) 1044
FbaI TGATCA 3 cut(s) 553, 862, 979
Fnu4HI GCNGC 3 cut(s) 37, 708, 935
FokI GGATG 3 cut(s) 433, 455, 943
Fsp4HI GCNGC 3 cut(s) 37, 708, 935
FspBI CTAG 2 cut(s) 680, 1032
GluI GCNGC 3 cut(s) 37, 708, 935
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 4 cut(s) 490, 542, 699, 732
HapII CCGG 3 cut(s) 532, 672, 928
Hin1II CATG 4 cut(s) 53, 509, 560, 615
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 3 cut(s) 1123, 1168, 1213
HinfI GANTC 9 cut(s) 181, 290, 317, 449, 776, 809, 818, 1055, 1103
HpaII CCGG 3 cut(s) 532, 672, 928
Hpy166II GTNNAC 2 cut(s) 829, 952
Hpy188I TCNGA 7 cut(s) 592, 622, 634, 751, 802, 814, 1060
Hpy188III TCNNGA 7 cut(s) 321, 392, 659, 860, 908, 928, 1222
Hpy8I GTNNAC 2 cut(s) 829, 952
Hpy99I CGWCG 1 cut(s) 690
HpyAV CCTTC 1 cut(s) 910
HpyCH4III ACNGT 1 cut(s) 956
HpyCH4IV ACGT 2 cut(s) 547, 1254
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 464, 931, 1007, 1135, 1144
HpyF3I CTNAG 3 cut(s) 372, 748, 801
HpySE526I ACGT 2 cut(s) 547, 1254
Hsp92II CATG 4 cut(s) 53, 509, 560, 615
Kpn2I TCCGGA 1 cut(s) 927
Ksp22I TGATCA 3 cut(s) 553, 862, 979
Kzo9I GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
LmnI GCTCC 2 cut(s) 930, 1024
Lsp1109I GCAGC 3 cut(s) 23, 694, 946
LweI GCATC 2 cut(s) 420, 921
MaeI CTAG 2 cut(s) 680, 1032
MaeII ACGT 2 cut(s) 547, 1254
MaeIII GTNAC 3 cut(s) 45, 350, 559
MalI GATC 9 cut(s) 139, 253, 405, 434, 555, 594, 753, 864, 981
MboI GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
MflI RGATCY 2 cut(s) 137, 592
MlyI GAGTC 2 cut(s) 299, 1112
MmeI TCCRAC 2 cut(s) 691, 873
MroI TCCGGA 1 cut(s) 927
MroXI GAANNNNTTC 1 cut(s) 993
MseI TTAA 3 cut(s) 1121, 1166, 1211
MslI CAYNNNNRTG 2 cut(s) 1157, 1202
MspCI CTTAAG 3 cut(s) 1120, 1165, 1210
MspI CCGG 3 cut(s) 532, 672, 928
MspR9I CCNGG 4 cut(s) 539, 672, 735, 1075
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 3 cut(s) 539, 735, 1075
MwoI GCNNNNNNNGC 6 cut(s) 15, 464, 931, 1007, 1135, 1144
NciI CCSGG 1 cut(s) 672
NdeII GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
NlaIII CATG 4 cut(s) 53, 509, 560, 615
NlaIV GGNNCC 2 cut(s) 536, 1263
NmuCI GTSAC 3 cut(s) 45, 350, 559
NspI RCATGY 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 392
PceI AGGCCT 1 cut(s) 490
PciI ACATGT 1 cut(s) 49
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 7 cut(s) 181, 317, 449, 776, 809, 818, 1055
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 3 cut(s) 38, 709, 936
PleI GAGTC 2 cut(s) 298, 1111
PpsI GAGTC 2 cut(s) 298, 1111
Ppu21I YACGTR 1 cut(s) 1255
PscI ACATGT 1 cut(s) 49
Psp1406I AACGTT 1 cut(s) 547
Psp6I CCWGG 3 cut(s) 537, 733, 1073
PspGI CCWGG 3 cut(s) 537, 733, 1073
PspN4I GGNNCC 2 cut(s) 536, 1263
PspPI GGNCC 3 cut(s) 534, 698, 731
PstNI CAGNNNCTG 3 cut(s) 812, 1144, 1189
PsuI RGATCY 2 cut(s) 137, 592
RsaI GTAC 2 cut(s) 530, 1100
RsaNI GTAC 2 cut(s) 529, 1099
RseI CAYNNNNRTG 2 cut(s) 1157, 1202
SaqAI TTAA 3 cut(s) 1121, 1166, 1211
SatI GCNGC 3 cut(s) 37, 708, 935
Sau3AI GATC 9 cut(s) 137, 251, 403, 432, 553, 592, 751, 862, 979
Sau96I GGNCC 3 cut(s) 534, 698, 731
SchI GAGTC 2 cut(s) 299, 1112
ScrFI CCNGG 4 cut(s) 539, 672, 735, 1075
SfaNI GCATC 2 cut(s) 420, 921
Sfr274I CTCGAG 1 cut(s) 392
SinI GGWCC 1 cut(s) 534
SlaI CTCGAG 1 cut(s) 392
SmiMI CAYNNNNRTG 2 cut(s) 1157, 1202
SmlI CTYRAG 4 cut(s) 392, 1120, 1165, 1210
SmoI CTYRAG 4 cut(s) 392, 1120, 1165, 1210
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 3 cut(s) 188, 574, 1051
SspMI CTAG 2 cut(s) 680, 1032
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 4 cut(s) 537, 670, 733, 1073
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 1 cut(s) 956
TaiI ACGT 2 cut(s) 550, 1257
TaqI TCGA 4 cut(s) 202, 393, 406, 435
TfiI GAWTC 7 cut(s) 181, 317, 449, 776, 809, 818, 1055
Tru1I TTAA 3 cut(s) 1121, 1166, 1211
Tru9I TTAA 3 cut(s) 1121, 1166, 1211
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 3 cut(s) 45, 350, 559
TseI GCWGC 3 cut(s) 36, 707, 934
Tsp45I GTSAC 3 cut(s) 45, 350, 559
TspDTI ATGAA 7 cut(s) 320, 441, 600, 774, 789, 948, 1056
TspGWI ACGGA 1 cut(s) 433
TspRI CASTG 1 cut(s) 138
Vha464I CTTAAG 3 cut(s) 1120, 1165, 1210
VpaK11BI GGWCC 1 cut(s) 534
XapI RAATTY 3 cut(s) 70, 992, 1225
XceI RCATGY 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 2 cut(s) 680, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.