Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0014042

Physical Location & Seq

Genomic Coordinates Forward (+)

3658262 .. 3663624

Gene Structure 5363 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGTCCATATCTTTTAGCTTCTCCAATCCTCAGTTTTCCTTTCCGTCAAACCTTATAAAAGTGTCCGATTCCAAACCCTGCAAAATCCCTTTTACTCCCTCCACCCTTCCCCCCTCCCATAACGACGTCGTCTCCCCTTTTCCCGCCACAGTGGGACCCATGAATCCCTCCACTCTCACTCCCCACCGCTCCGCTTTCCGCTCGCCCTTCCTCAACCCCATTTCTCTCCCCGCCGCAACCTCCGTACACTCCCGACGACGCCGTTTCATGGTCTCATTTCCCCGGAACTCTGCCGCCTCCTCGGAACAATCAGCAGATGCTTCACCGCCGCCTCCCGATGTCTTCGGCGGCAAGAGAGAGCTCACCGGTATCCAGCCCGTTGTGGAGAAGCTTTCGCCGCCCCTCCGGATAGTGACGTCGGCAATTGTGTTCGCTGGAGCTGTGGCTGCGGGTTATGGGTTGGGTCTCCGGTTGGGGAAGTCCCAAAACGCAGCTTATGGCGGTGCAGCGGTGCTTGGAGCCGCCGGTGGGGCCGCCTTATACGCTATGAACTCTTGTGCTCCCGAGGTCGCCGCGGTTGACTTGCACAATTACGTTGCCGGGTTCGACGACCCGAAAGCTGTTAAGAAGGAGGACATTGAAGGCATTGCGAGAAAATATGGTGTGAGCAAGCAAGACGAGGCGTTTAATGCCGAGCTCTGTGATTTGTACTGTCGTTTTGTCACTTCTGTTCTTCCTCCTGGAGCTCAAGAACTCAAAGGGGACGAAGTCGACACAATCGTCAGCTTCAAGAATTCTCTGGGAATCGATGACCCCGAAGCAGCTTCAATGCATATGGAGATTGGAAGGCGAATTTTCAGACAGAGGCTCGAGACTGACCGTGAGGGTGATCTAGAGCAGCGTCGGGCGTTTCAGAAGCTGATATATGTTTCGACTCTTGTGTTTGGGGATGCTTCATCTTTCCTCTTACCTTGGAAGCGTGTTTTCAAAGTTACTGATTCCCAGGTTGAGCTGGCTATCCGTGATAATGCCCAGCGTTTATACGCTTCCAAGTTAAAATCCGTTGGCAGAGATATTGGAGTAGAGCAGCTTGTTAAGCTTAAAGAAGCACAACTCATGTATCGGCTCTCTGACGAGCACGCAGAGGACTTGTTCAAGGAACATGCAAGAAAACTAGTTGAGGCAAATATTTCAGCAGCACTTCGTATACTCAAGTCCAGAACCAGATCAGCTGCTGGAATCATGGAAGTTGTGGAAGAGCTCGATAAAATGTTAGAACTCAATAGTTTGCTCATCTCACTAAAGAACCAGCCAGATGCAGCTCGCTTTGCACCTGGGGTTGGGCCGGTTTCTTTACTTGGTGGCGACTACGATGCTGATAGAAAGATGGATGACTTGAAGCTCCTCTTCAGAGCATATGTTACGGATTCTTTATCCACAGGTTGCTTGGAAGAGAGTAAGCTTTCGGCCTTGAATCAGTTAAGGAATATATTTGGTTTGGGCAAACGAGAGGCAGAATCCATTGTGCTTGATGTTACTTTGAAGGTATACCGTAAACGTCTTTCACAATCTGTTACATCGGGTGAGTTAGAAGCCGCAGACAGCAAAGCAGCCTTCCTTCAAAATATCTGTGAGGAGCTGCATTTTGATCCACAAAGAGCCAGCCAGATTCATGAAGAAATTTACCGGCAAAAGCTTCAGCAGTGTGTTGCTGATGGAGAGCTGAATGAGGATGATGTTGCTGTATTGTTAAGGCTACGGGTCATGCTTTGTATACCTCAGCAGACAGTTGAGGCTGCCCATTCAGATATCTGTGGCAGTTTGTTTGAAAAGGTTGTGAAGGACGCAATTGCATCTGGTGTTGATGGTTACGACGCTGATGTAAAACAAGCCGTGAGAAAGGCAGCTCATGGTCTGCGATTGTCTAGGGAGGCTGCCATGTCTATTGCTGGCAAGGCAGTAAGAAAGATTTTTATTAACTATGTAAAACGAGCGCGGTCAGTTGGTAGCCGTACTGAGTCTGCAAAAGAACTTAAAAAAATGATAGCCTTCAACACATTGGTTGTGACTGAATTGGTGGCAGACATAAAAGGGGAATCATCTGATTCCGCCACAGAAGAGCCAATAAAGGAGCCAGAGACAGAAGTCCTGGAGGATGAGGAATGGGAATCTATTCAGACACTTCGGAAGATTAGACCTGACAAGGAGCTTACTGCAAAGTTGGGGAAGCCCGGCCAGACTGAGATAACTCTTAAAGATGACCTGGAAGAAAGAGAGAGGACTGACCTATACAAAACATACTTGCTGTTTTGTATAACTGGGGAAGTGAAGAGGATTCCCTTTGGTGCTCAAATCACTACAAAGAAGGATGATTCAGAATATGTGCTACTAAATCAGCTTGGGGCGATATTGGGTTTAAGCATTACGGAGATAGTGGAAGTACACAGGAGCCTGGCCGAGCAAGCTTTCAGGCAACAGGCAGAGGTGATTTTAGCTGATGGACAATTGACAAAAGCCAGGGTAGAGCAACTTAATGAATTGCAGAAGCAAGTTGGCTTGCCTCCGCAGTACGTGGAAAAGATAATAAAGAATATAACTACTACAAAAATGGCAGCTGCGATTGAAACCGCTATTGGTCAGGGGAGGCTCAATATTAAGCAGATAAGAGAACTTAAAGAATCAAGTGTTAATTTAGACAGCATGATATCTAAGAGCTTGCGAGAAAGTCTGTTCAAAAAGACCGTGGATGAGATATTCTCATCAGGCACTGGAGAGTTTGATGAGGAAGAAGTCTATGAGAAAATCCCAGCAGATCTCAATATTAATGCTGAGAAGGCCAAGAATGTTGTTCGAGAGCTTGCACGGACCAGATTATCAAACTCACTGATTCAAGCTGTGTCACTTCTGAGGCAGAGAAATCGTCAAGGAGTGGTTTCCTCTCTTAATGACTTGCTAGCTTGCGACAAAGCTGTGCCTGCCAAGCCACTTTCATGGGAAGTTCCAGAGGAGCTGGCCGATCTCTTTGGCATATACATGAAAAGTGATGCGGCACCAGAGAAATTATCGCGCTTGCAGTATCTCTTGGGCATTAGTGACAGCATGGCCACTGCTCTCCTGGAGATGGGAGATAGATTACAAACCATCGGTGCGGAGGAGGAAAAGTTTGTATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009658 GO:0009706 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019866 GO:0031090 GO:0031897 GO:0031967 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tic110

Protein Analysis

1058

Amino Acids

116.59

Weight (kDa)

5.8

Isoelectric Point (pI)

45.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tic110	PF16940	121 - 693	5.6e-288	Chloroplast envelope transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014042)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06950
fragaria_vesca	FvH4_3g40080
malus_domestica	MD03G1058800.v1.1 MD11G1060900.v1.1
prunus_persica	Prupe.6G045000_v2.0.a1
pyrus_communis	pycom03g04710 pycom11g05040
rosa_chinensis	RchiOBHm_Chr5g0072271
rosa_laevigata	RLG00000036290
rosa_multiflora	Rmu_sc0000714.1_g000010
rosa_roxburghii	Rroxscaffold_1G00009000
rosa_rugosa	Rorug05G0417400
rosa_samantha	Rh5AG473200 Rh5BG493500 Rh5CG517500 Rh5DG505400
rosa_wichuraiana	Rw5G043970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	56
AasI	GACNNNNNNGTC	1 cut(s)	779
AatII	GACGTC	2 cut(s)	129, 419
AccB1I	GGYRCC	1 cut(s)	3049
AccBSI	CCGCTC	2 cut(s)	189, 201
AccI	GTMKAC	4 cut(s)	771, 1207, 1548, 1774
AccII	CGCG	3 cut(s)	575, 1996, 3067
AccIII	TCCGGA	1 cut(s)	405
AclWI	GGATC	1 cut(s)	1643
AcoI	YGGCCR	4 cut(s)	2233, 2454, 3012, 3102
AcsI	RAATTY	3 cut(s)	793, 852, 1680
AcuI	CTGAAG	2 cut(s)	1393, 1682
AcyI	GRCGYC	3 cut(s)	126, 259, 416
AfaI	GTAC	5 cut(s)	246, 710, 2014, 2442, 2570
AfiI	CCNNNNNNNGG	4 cut(s)	268, 528, 1340, 3121
AgeI	ACCGGT	1 cut(s)	365
AhlI	ACTAGT	1 cut(s)	1174
AjnI	CCWGG	8 cut(s)	739, 1002, 1333, 2148, 2262, 2451, 2516, 3114
AjuI	GAANNNNNNNTTGG	2 cut(s)	2616, 2648
Alw21I	GWGCWC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
Alw26I	GTCTC	5 cut(s)	136, 277, 470, 866, 2132
AlwI	GGATC	1 cut(s)	1643
AlwNI	CAGNNNCTG	3 cut(s)	919, 1235, 2768
Ama87I	CYCGRG	2 cut(s)	563, 869
Aor13HI	TCCGGA	1 cut(s)	405
AoxI	GGCC	8 cut(s)	531, 1343, 1467, 2233, 2454, 2835, 3012, 3102
ApoI	RAATTY	3 cut(s)	793, 852, 1680
AseI	ATTAAT	1 cut(s)	2823
AsiGI	ACCGGT	1 cut(s)	365
Asp700I	GAANNNNTTC	1 cut(s)	2172
AspLEI	GCGC	2 cut(s)	1996, 3069
AspS9I	GGNCC	4 cut(s)	155, 531, 1343, 2865
AsuC2I	CCSGG	3 cut(s)	283, 601, 2232
AsuHPI	GGTGA	5 cut(s)	315, 355, 899, 1595, 2497
AsuNHI	GCTAGC	1 cut(s)	2953
AvaI	CYCGRG	2 cut(s)	563, 869
AvaII	GGWCC	2 cut(s)	155, 2865
BaeI	ACNNNNGTAYC	2 cut(s)	1103, 1136
BalI	TGGCCA	1 cut(s)	3104
BanI	GGYRCC	1 cut(s)	3049
BanII	GRGCYC	4 cut(s)	363, 699, 748, 1263
BbsI	GAAGAC	1 cut(s)	334
Bbv12I	GWGCWC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
BbvCI	CCTCAGC	1 cut(s)	1779
BccI	CCATC	6 cut(s)	1381, 1709, 1859, 2492, 3115, 3149
BceAI	ACGGC	3 cut(s)	246, 1877, 1995
BcgI	CGANNNNNNTGC	4 cut(s)	1355, 1389, 2900, 2934
BciT130I	CCWGG	8 cut(s)	741, 1004, 1335, 2150, 2264, 2453, 2518, 3116
BciVI	GTATCC	1 cut(s)	380
BcnI	CCSGG	3 cut(s)	283, 601, 2232
BcoDI	GTCTC	5 cut(s)	136, 277, 470, 866, 2132
BcuI	ACTAGT	1 cut(s)	1174
BfaI	CTAG	4 cut(s)	893, 1175, 1926, 2954
BfuI	GTATCC	1 cut(s)	380
BglI	GCCNNNNNGGC	1 cut(s)	530
BglII	AGATCT	1 cut(s)	2812
Bme18I	GGWCC	2 cut(s)	155, 2865
BmeT110I	CYCGRG	2 cut(s)	563, 869
BmgT120I	GGNCC	4 cut(s)	155, 531, 1343, 2865
BmiI	GGNNCC	7 cut(s)	156, 157, 520, 532, 2133, 2450, 3051
BmrI	ACTGGG	1 cut(s)	2328
BmsI	GCATC	6 cut(s)	307, 940, 1306, 1363, 1862, 3034
BmtI	GCTAGC	1 cut(s)	2957
BmuI	ACTGGG	1 cut(s)	2328
BoxI	GACNNNNGTC	1 cut(s)	2144
BpiI	GAAGAC	1 cut(s)	334
BplI	GAGNNNNNCTC	2 cut(s)	2741, 2773
BpmI	CTGGAG	5 cut(s)	456, 762, 2171, 2790, 3137
Bpu10I	CCTNAGC	1 cut(s)	1779
BpuEI	CTTGAG	2 cut(s)	732, 1196
BpuMI	CCSGG	3 cut(s)	283, 601, 2232
Bsa29I	ATCGAT	1 cut(s)	807
BsaAI	YACGTR	1 cut(s)	2572
BsaHI	GRCGYC	3 cut(s)	126, 259, 416
BsaI	GGTCTC	2 cut(s)	277, 470
BsaJI	CCNNGG	9 cut(s)	281, 300, 564, 573, 971, 1002, 1334, 2517, 2742
BsaWI	WCCGGW	3 cut(s)	365, 405, 468
BsaXI	ACNNNNNCTCC	5 cut(s)	116, 146, 163, 193, 3146
Bsc4I	CCNNNNNNNGG	4 cut(s)	268, 528, 1340, 3121
Bse118I	RCCGGY	4 cut(s)	365, 524, 1345, 1686
Bse1I	ACTGG	2 cut(s)	2323, 2773
Bse3DI	GCAATG	1 cut(s)	645
BseAI	TCCGGA	1 cut(s)	405
BseBI	CCWGG	8 cut(s)	741, 1004, 1335, 2150, 2264, 2453, 2518, 3116
BseCI	ATCGAT	1 cut(s)	807
BseDI	CCNNGG	9 cut(s)	281, 300, 564, 573, 971, 1002, 1334, 2517, 2742
BseGI	GGATG	6 cut(s)	955, 1396, 1738, 2161, 2374, 2752
BseLI	CCNNNNNNNGG	4 cut(s)	268, 528, 1340, 3121
BseMI	GCAATG	1 cut(s)	645
BseMII	CTCAG	6 cut(s)	44, 1793, 2007, 2232, 2820, 2897
BseNI	ACTGG	2 cut(s)	2323, 2773
BseRI	GAGGAG	5 cut(s)	289, 1394, 1649, 3020, 3167
BseYI	CCCAGC	2 cut(s)	1032, 2806
BsgI	GTGCAG	1 cut(s)	525
Bsh1236I	CGCG	3 cut(s)	575, 1996, 3067
BshFI	GGCC	8 cut(s)	533, 1345, 1469, 2235, 2456, 2837, 3014, 3104
BshNI	GGYRCC	1 cut(s)	3049
BshTI	ACCGGT	1 cut(s)	365
BshVI	ATCGAT	1 cut(s)	807
BsiHKAI	GWGCWC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
BsiHKCI	CYCGRG	2 cut(s)	563, 869
BsiSI	CCGG	9 cut(s)	283, 366, 406, 469, 525, 600, 1346, 1687, 2232
BslFI	GGGAC	3 cut(s)	168, 466, 776
BslI	CCNNNNNNNGG	4 cut(s)	268, 528, 1340, 3121
BsmAI	GTCTC	5 cut(s)	136, 277, 470, 866, 2132
BsmBI	CGTCTC	1 cut(s)	136
BsmFI	GGGAC	3 cut(s)	168, 466, 776
BsnI	GGCC	8 cut(s)	533, 1345, 1469, 2235, 2456, 2837, 3014, 3104
Bso31I	GGTCTC	2 cut(s)	277, 470
BsoBI	CYCGRG	2 cut(s)	563, 869
Bsp1286I	GDGCHC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
Bsp13I	TCCGGA	1 cut(s)	405
Bsp143I	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
BspANI	GGCC	8 cut(s)	533, 1345, 1469, 2235, 2456, 2837, 3014, 3104
BspCNI	CTCAG	6 cut(s)	43, 1792, 2008, 2233, 2821, 2898
BspDI	ATCGAT	1 cut(s)	807
BspEI	TCCGGA	1 cut(s)	405
BspFNI	CGCG	3 cut(s)	575, 1996, 3067
BspHI	TCATGA	1 cut(s)	1672
BspLI	GGNNCC	7 cut(s)	156, 157, 520, 532, 2133, 2450, 3051
BspOI	GCTAGC	1 cut(s)	2957
BspPI	GGATC	1 cut(s)	1643
BspQI	GCTCTTC	2 cut(s)	1251, 2112
BspT107I	GGYRCC	1 cut(s)	3049
BspTNI	GGTCTC	2 cut(s)	277, 470
BsrBI	CCGCTC	2 cut(s)	189, 201
BsrDI	GCAATG	1 cut(s)	645
BsrFI	RCCGGY	4 cut(s)	365, 524, 1345, 1686
BsrI	ACTGG	2 cut(s)	2323, 2773
BssAI	RCCGGY	4 cut(s)	365, 524, 1345, 1686
BssECI	CCNNGG	9 cut(s)	281, 300, 564, 573, 971, 1002, 1334, 2517, 2742
BssMI	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
BssNAI	GTATAC	3 cut(s)	1208, 1549, 1775
BssNI	GRCGYC	3 cut(s)	126, 259, 416
BssT1I	CCWWGG	1 cut(s)	971
Bst1107I	GTATAC	3 cut(s)	1208, 1549, 1775
Bst2UI	CCWGG	8 cut(s)	741, 1004, 1335, 2150, 2264, 2453, 2518, 3116
Bst4CI	ACNGT	6 cut(s)	151, 713, 881, 1553, 1789, 2743
Bst6I	CTCTTC	5 cut(s)	1251, 1412, 1446, 2112, 2324
BstACI	GRCGYC	3 cut(s)	126, 259, 416
BstBAI	YACGTR	1 cut(s)	2572
BstDEI	CTNAG	7 cut(s)	30, 1779, 2016, 2241, 2709, 2829, 2906
BstDSI	CCRYGG	2 cut(s)	573, 2742
BstF5I	GGATG	6 cut(s)	955, 1396, 1738, 2161, 2374, 2752
BstFNI	CGCG	3 cut(s)	575, 1996, 3067
BstHHI	GCGC	2 cut(s)	1996, 3069
BstKTI	GATC	5 cut(s)	892, 1229, 1651, 2815, 3019
BstMAI	GTCTC	5 cut(s)	136, 277, 470, 866, 2132
BstMBI	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
BstNI	CCWGG	8 cut(s)	741, 1004, 1335, 2150, 2264, 2453, 2518, 3116
BstNSI	RCATGY	1 cut(s)	1166
BstPAI	GACNNNNGTC	1 cut(s)	2144
BstUI	CGCG	3 cut(s)	575, 1996, 3067
BstV2I	GAAGAC	1 cut(s)	334
BstX2I	RGATCY	1 cut(s)	2812
BstXI	CCANNNNNNTGG	1 cut(s)	2991
BstYI	RGATCY	1 cut(s)	2812
BstZ17I	GTATAC	3 cut(s)	1208, 1549, 1775
Bsu15I	ATCGAT	1 cut(s)	807
BsuI	GTATCC	1 cut(s)	380
BsuRI	GGCC	8 cut(s)	533, 1345, 1469, 2235, 2456, 2837, 3014, 3104
BsuTUI	ATCGAT	1 cut(s)	807
BtgI	CCRYGG	2 cut(s)	573, 2742
BtsCI	GGATG	6 cut(s)	955, 1396, 1738, 2161, 2374, 2752
BtsI	GCAGTG	2 cut(s)	1709, 3105
BtsIMutI	CAGTG	5 cut(s)	156, 1709, 2766, 2882, 3105
CaiI	CAGNNNCTG	3 cut(s)	919, 1235, 2768
CciI	TCATGA	1 cut(s)	1672
CfoI	GCGC	2 cut(s)	1996, 3069
Cfr10I	RCCGGY	4 cut(s)	365, 524, 1345, 1686
Cfr13I	GGNCC	4 cut(s)	155, 531, 1343, 2865
Cfr42I	CCGCGG	1 cut(s)	576
ClaI	ATCGAT	1 cut(s)	807
CseI	GACGC	4 cut(s)	267, 890, 1853, 1883
Csp6I	GTAC	5 cut(s)	245, 709, 2013, 2441, 2569
CspAI	ACCGGT	1 cut(s)	365
CviQI	GTAC	5 cut(s)	245, 709, 2013, 2441, 2569
DdeI	CTNAG	7 cut(s)	30, 1779, 2016, 2241, 2709, 2829, 2906
DpnI	GATC	5 cut(s)	891, 1228, 1650, 2814, 3018
DpnII	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
DrdI	GACNNNNNNGTC	1 cut(s)	779
DseDI	GACNNNNNNGTC	1 cut(s)	779
EaeI	YGGCCR	4 cut(s)	2233, 2454, 3012, 3102
Eam1104I	CTCTTC	5 cut(s)	1251, 1412, 1446, 2112, 2324
EarI	CTCTTC	5 cut(s)	1251, 1412, 1446, 2112, 2324
EciI	GGCGGA	1 cut(s)	2098
Ecl136II	GAGCTC	4 cut(s)	361, 697, 746, 1261
Eco130I	CCWWGG	1 cut(s)	971
Eco24I	GRGCYC	4 cut(s)	363, 699, 748, 1263
Eco31I	GGTCTC	2 cut(s)	277, 470
Eco32I	GATATC	2 cut(s)	1810, 2706
Eco47I	GGWCC	2 cut(s)	155, 2865
Eco53kI	GAGCTC	4 cut(s)	361, 697, 746, 1261
Eco57I	CTGAAG	2 cut(s)	1393, 1682
Eco88I	CYCGRG	2 cut(s)	563, 869
EcoICRI	GAGCTC	4 cut(s)	361, 697, 746, 1261
EcoO109I	RGGNCCY	1 cut(s)	155
EcoRI	GAATTC	1 cut(s)	793
EcoRII	CCWGG	8 cut(s)	739, 1002, 1333, 2148, 2262, 2451, 2516, 3114
EcoRV	GATATC	2 cut(s)	1810, 2706
EcoT14I	CCWWGG	1 cut(s)	971
EcoT22I	ATGCAT	1 cut(s)	834
EcoT38I	GRGCYC	4 cut(s)	363, 699, 748, 1263
ErhI	CCWWGG	1 cut(s)	971
Esp3I	CGTCTC	1 cut(s)	136
FalI	AAGNNNNNCTT	2 cut(s)	1391, 1423
FaqI	GGGAC	3 cut(s)	168, 466, 776
FauI	CCCGC	3 cut(s)	151, 238, 442
FauNDI	CATATG	2 cut(s)	834, 1417
FblI	GTMKAC	4 cut(s)	771, 1207, 1548, 1774
FokI	GGATG	6 cut(s)	962, 1403, 1745, 2168, 2381, 2759
FriOI	GRGCYC	4 cut(s)	363, 699, 748, 1263
FspBI	CTAG	4 cut(s)	893, 1175, 1926, 2954
GlaI	GCGC	2 cut(s)	1995, 3068
GsaI	CCCAGC	2 cut(s)	1036, 2810
GsuI	CTGGAG	5 cut(s)	456, 762, 2171, 2790, 3137
HaeIII	GGCC	8 cut(s)	533, 1345, 1469, 2235, 2456, 2837, 3014, 3104
HapII	CCGG	9 cut(s)	283, 366, 406, 469, 525, 600, 1346, 1687, 2232
HgaI	GACGC	4 cut(s)	267, 890, 1853, 1883
HhaI	GCGC	2 cut(s)	1996, 3069
Hin1I	GRCGYC	3 cut(s)	126, 259, 416
Hin6I	GCGC	2 cut(s)	1994, 3067
HinP1I	GCGC	2 cut(s)	1994, 3067
HincII	GTYRAC	2 cut(s)	580, 772
HindII	GTYRAC	2 cut(s)	580, 772
HindIII	AAGCTT	5 cut(s)	389, 1097, 1460, 1694, 2463
HpaII	CCGG	9 cut(s)	283, 366, 406, 469, 525, 600, 1346, 1687, 2232
HphI	GGTGA	5 cut(s)	315, 355, 899, 1595, 2497
Hpy166II	GTNNAC	8 cut(s)	247, 580, 772, 1208, 1549, 1556, 1775, 2443
Hpy8I	GTNNAC	8 cut(s)	247, 580, 772, 1208, 1549, 1556, 1775, 2443
Hpy99I	CGWCG	8 cut(s)	128, 131, 258, 261, 421, 611, 906, 1877
HpyCH4III	ACNGT	6 cut(s)	151, 713, 881, 1553, 1789, 2743
HpyCH4IV	ACGT	5 cut(s)	126, 416, 594, 1558, 2571
HpyF3I	CTNAG	7 cut(s)	30, 1779, 2016, 2241, 2709, 2829, 2906
HpySE526I	ACGT	5 cut(s)	126, 416, 594, 1558, 2571
Hsp92I	GRCGYC	3 cut(s)	126, 259, 416
HspAI	GCGC	2 cut(s)	1994, 3067
KflI	GGGWCCC	1 cut(s)	155
Kpn2I	TCCGGA	1 cut(s)	405
KspI	CCGCGG	1 cut(s)	576
Kzo9I	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
LguI	GCTCTTC	2 cut(s)	1251, 2112
LweI	GCATC	6 cut(s)	307, 940, 1306, 1363, 1862, 3034
MaeI	CTAG	4 cut(s)	893, 1175, 1926, 2954
MaeII	ACGT	5 cut(s)	126, 416, 594, 1558, 2571
MalI	GATC	5 cut(s)	891, 1228, 1650, 2814, 3018
MbiI	CCGCTC	2 cut(s)	189, 201
MboI	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
MfeI	CAATTG	3 cut(s)	423, 1848, 2504
MflI	RGATCY	1 cut(s)	2812
MhlI	GDGCHC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
MlsI	TGGCCA	1 cut(s)	3104
MluNI	TGGCCA	1 cut(s)	3104
MlyI	GAGTC	2 cut(s)	928, 2027
Mox20I	TGGCCA	1 cut(s)	3104
Mph1103I	ATGCAT	1 cut(s)	834
MroI	TCCGGA	1 cut(s)	405
MroXI	GAANNNNTTC	1 cut(s)	2172
MscI	TGGCCA	1 cut(s)	3104
MslI	CAYNNNNRTG	2 cut(s)	2989, 3032
Msp20I	TGGCCA	1 cut(s)	3104
MspA1I	CMGCKG	4 cut(s)	509, 575, 1232, 2615
MspI	CCGG	9 cut(s)	283, 366, 406, 469, 525, 600, 1346, 1687, 2232
MunI	CAATTG	3 cut(s)	423, 1848, 2504
MvaI	CCWGG	8 cut(s)	741, 1004, 1335, 2150, 2264, 2453, 2518, 3116
MvnI	CGCG	3 cut(s)	575, 1996, 3067
NciI	CCSGG	3 cut(s)	283, 601, 2232
NdeI	CATATG	2 cut(s)	834, 1417
NdeII	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
NheI	GCTAGC	1 cut(s)	2953
NlaIV	GGNNCC	7 cut(s)	156, 157, 520, 532, 2133, 2450, 3051
NmeAIII	GCCGAG	2 cut(s)	718, 2482
NmuCI	GTSAC	5 cut(s)	412, 721, 2065, 2898, 3092
NsiI	ATGCAT	1 cut(s)	834
NspI	RCATGY	1 cut(s)	1166
PaeR7I	CTCGAG	1 cut(s)	869
PagI	TCATGA	1 cut(s)	1672
PciSI	GCTCTTC	2 cut(s)	1251, 2112
PcsI	WCGNNNNNNNCGW	2 cut(s)	777, 813
PdmI	GAANNNNTTC	1 cut(s)	2172
PflFI	GACNNNGTC	2 cut(s)	128, 767
PfoI	TCCNGGA	3 cut(s)	739, 2148, 3114
PinAI	ACCGGT	1 cut(s)	365
PleI	GAGTC	2 cut(s)	928, 2026
PpsI	GAGTC	2 cut(s)	928, 2026
Ppu21I	YACGTR	1 cut(s)	2572
PpuMI	RGGWCCY	1 cut(s)	155
PshAI	GACNNNNGTC	1 cut(s)	2144
PshBI	ATTAAT	1 cut(s)	2823
PsiI	TTATAA	1 cut(s)	56
Psp124BI	GAGCTC	4 cut(s)	363, 699, 748, 1263
Psp5II	RGGWCCY	1 cut(s)	155
Psp6I	CCWGG	8 cut(s)	739, 1002, 1333, 2148, 2262, 2451, 2516, 3114
PspFI	CCCAGC	2 cut(s)	1032, 2806
PspGI	CCWGG	8 cut(s)	739, 1002, 1333, 2148, 2262, 2451, 2516, 3114
PspN4I	GGNNCC	7 cut(s)	156, 157, 520, 532, 2133, 2450, 3051
PspPI	GGNCC	4 cut(s)	155, 531, 1343, 2865
PspPPI	RGGWCCY	1 cut(s)	155
PstNI	CAGNNNCTG	3 cut(s)	919, 1235, 2768
PsuI	RGATCY	1 cut(s)	2812
PsyI	GACNNNGTC	2 cut(s)	128, 767
PvuII	CAGCTG	2 cut(s)	1232, 2615
RsaI	GTAC	5 cut(s)	246, 710, 2014, 2442, 2570
RsaNI	GTAC	5 cut(s)	245, 709, 2013, 2441, 2569
RseI	CAYNNNNRTG	2 cut(s)	2989, 3032
SacI	GAGCTC	4 cut(s)	363, 699, 748, 1263
SacII	CCGCGG	1 cut(s)	576
SalI	GTCGAC	1 cut(s)	770
SapI	GCTCTTC	2 cut(s)	1251, 2112
Sau3AI	GATC	5 cut(s)	889, 1226, 1648, 2812, 3016
Sau96I	GGNCC	4 cut(s)	155, 531, 1343, 2865
SchI	GAGTC	2 cut(s)	928, 2027
SduI	GDGCHC	7 cut(s)	363, 562, 699, 748, 1140, 1263, 2350
SfaNI	GCATC	6 cut(s)	307, 940, 1306, 1363, 1862, 3034
Sfr274I	CTCGAG	1 cut(s)	869
Sfr303I	CCGCGG	1 cut(s)	576
SgrAI	CRCCGGYG	1 cut(s)	524
SgrBI	CCGCGG	1 cut(s)	576
SinI	GGWCC	2 cut(s)	155, 2865
SlaI	CTCGAG	1 cut(s)	869
SmiMI	CAYNNNNRTG	2 cut(s)	2989, 3032
SmlI	CTYRAG	3 cut(s)	747, 869, 1211
SmoI	CTYRAG	3 cut(s)	747, 869, 1211
SpeI	ACTAGT	1 cut(s)	1174
SspI	AATATT	3 cut(s)	1189, 2653, 2821
SspMI	CTAG	4 cut(s)	893, 1175, 1926, 2954
SstI	GAGCTC	4 cut(s)	363, 699, 748, 1263
StyI	CCWWGG	1 cut(s)	971
TaaI	ACNGT	6 cut(s)	151, 713, 881, 1553, 1789, 2743
TaiI	ACGT	5 cut(s)	129, 419, 597, 1561, 2574
TaqI	TCGA	7 cut(s)	606, 771, 807, 870, 932, 1263, 2851
TatI	WGTACW	2 cut(s)	708, 2440
TscAI	CASTG	5 cut(s)	156, 1709, 2773, 2889, 3112
TseFI	GTSAC	5 cut(s)	412, 721, 2065, 2898, 3092
Tsp45I	GTSAC	5 cut(s)	412, 721, 2065, 2898, 3092
TspDTI	ATGAA	9 cut(s)	176, 256, 563, 945, 1661, 1689, 2550, 2979, 3050
TspGWI	ACGGA	7 cut(s)	33, 232, 1010, 1051, 1439, 2441, 2878
TspRI	CASTG	5 cut(s)	156, 1709, 2773, 2889, 3112
Tth111I	GACNNNGTC	2 cut(s)	128, 767
VpaK11BI	GGWCC	2 cut(s)	155, 2865
VspI	ATTAAT	1 cut(s)	2823
XapI	RAATTY	3 cut(s)	793, 852, 1680
XbaI	TCTAGA	1 cut(s)	892
XceI	RCATGY	1 cut(s)	1166
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1444, 3112
XhoI	CTCGAG	1 cut(s)	869
XmiI	GTMKAC	4 cut(s)	771, 1207, 1548, 1774
XmnI	GAANNNNTTC	1 cut(s)	2172
XspI	CTAG	4 cut(s)	893, 1175, 1926, 2954
ZraI	GACGTC	2 cut(s)	127, 417
Zsp2I	ATGCAT	1 cut(s)	834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.