pycom03g05520

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010550
Physical Location & Seq
Reverse (-)
4360640 .. 4369141
8502 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3441 bp
ATGGGGAGTTTTAGGATGTGCATGTGCTTCACTAGGAAGTTCAGGGTGAAGGAGGAAGAGCCACCGCGGGACGTGAAGGAAGCATTCGAAAAGTACGCTGAGGGGGGGACCCAAATGACGGCGGAGCAGCTGCGCCGCTTCTTGGCCGAGCTCCAGGGGGAGAAAGATGGCGGCGGCGCGTCGGTATCAGACGACGCGGAGCGGATCGTGGAGCAGGTGCTGCAGAAGAGGCACCACATTGCCAAGTTGATCACAAAGCGCACTCTGTCCCTTGAAGACTTCCACCATTACTTGTTCTCTCCCGATCTCAATCCCCCTATTCGAGATCAGGTTCACCAGGACATGACAGCTCCGTTGTCCCATTACTACATATATACCGGCCATAATTCGTACCTGACTGGAAATCAGCTAAGTAGTGACTGCAGCGACGTTCCGATCATAAAGGCACTCAAAAGAGGTGTGAGGGTGGTGGAGCTTGATATTTGGCCAAATTCCACAAAGGACAATGTGCATGTTCTCCACGGAAGGACCTTGACAACCCCTGTGGAACTTATAAAATGCTTGAAATCAATTAAAGAGCATGCCTTTTCTGAATCTCCATACCCCGTCATCATAACTCTTGAAGACCACCTTACGGCTGATCTCCAAGCTAAAGTAGCACTGATGCTCATTCAAACATTTGGTGAAATGTTGTTTTACCCTGAAACCTGCTGTTTGAAAGAGTTACCTTCGCCCGAAGAACTTAAATATCGAATTATTATATCTACAAAACCTCCAGAGGAGTACCTTAAGGATAAAACTTCCAATGAAAATGGACTTGACTCACATAAAAGTGAGGAAGATTTATGGGGGAAGGAACCATCAGAGCTTACCCATGAACGGGAAGATGATGATACGAGTGACAGTGATACAAGTGAGGACAACAATAATAACAATCATGGATCGTTCTCCTCAGTAGAACACGATTACAAATGTCTAATTGCAATCCATGCTGGAAAACCCAAGGGTGGTTTAAAGGACGCGCTAAAAGTTGAACTCGATAAAGTCAGGCGGCTTAGCTTGAGTGAACAAGCACTCGAGAAGGCTGCTGAATCTCATGGAACAGATATTGTTAGATTTACACAGAAGAACATTTTAAGGGTGTATCCAAAAGGTACTCGGTTCAACTCCTCGAACTACAAGCCGCTTGTTGGTTGGATGCATGGTGCTCAAATGGTTGCGTTCAATATGCAGGGATATGGTAGATCTCTTTGGCTGATGCATGGGATGTTTAGAGCCAATGGGGGTTGCGGTTATGTTAAAAAGCCTGATTTTGTTATGAAAGCGGATTCCGATTATCAGGTTTTCGATCCTAAAGCAAAACTGCCAGTAAAGAAGACTTTAAAGGTGAAAGTTTATATGGGAGATGGATGGCATTTGGATTTCAAACAAACACACTTTGATTTATATTCTCCACCAGATTTCTATACTAGGGTTGGCATAGCAGGAGTGCCGGCAGATGAAATAATGAAGAAAACGAAGAAAAAAGAGGACGATTGGACACCTGTATGGGAGGAAGAGTTTACATTTCCATTGACAGTTCCCGAGTTGGCTTTGCTTAGAGTTGAGGTACATGAGTATGACATGTCTGAGAAAGATGATTTTGGTGGCCAAACTTGTTTGCCAGTCTCCGAATTGAGACAAGGGATTCGTGCAGTCCCTCTCTTTGATCGCAAAGGAGTAAAATACAATTCAGTAAGGCTTCTAATGCGATTTGAGTTCAAAGCAGTTTCAAAAATGTCTAATAAGCAGTCTTTCGATGTCTGCTTTTGCTTCCATAGAAAATTTAGGCTCCGGATGGCAGAGCCTCCTGAAGATATTAAGAACCTTTTTGAAAAATTCGCAGAAAATGGCACCATGACCATCGATGACCTGCAGTGTTTTCTGATTGAGTTTCAAGGAGAATCCAGTGCCACCAAGGAGGATGCTCAGACCATTTTCAATAGTCTCAAGCATCTCAACATCTTTCAACGAAAGGGTCTCCACCTTGATGCCTTCTTTCGATATCTCCTCGGCAACCTTAATCCTCCTCTCTATTCAAAGGTGCACCATGACATGAATTCTCCATTGGCTCATTATTTCCTATTTACCGGCCACAACTCTTACTTAACAGGAAATCAATTCAGCAGTAACAGTAGTATACATCCGATAATTAACGCGCTGCTTCAAGGTGTTAGGGTTATTGAATTAGACTTGTGGCCGGGTTCTAAGAAAAATACTGTGGAGGTTCGTCACGGAGGGACGCTTACAAGTCCGGTGGACCTCATCAAATGTTTGCGCGCGATCAAGGACAACGCTTTTGTTGCTTCTGAGTACCCTGTTATCATAACATTTGAAGACCACCTACCTTCAAATCTTCAAGCCAAAGTGGCAAAGATGGTCACTGGGACATTCGGTGACATGCTGTACTCTCCTCATTCAGAATTCTTAATGGAATTCCCTTCACCGGAATCATTGAAGAGGAGGATTTTGATTTCGACCAAGCCACCGGAGTATCATGAATCACAGAGACGGAGAGATGAATCTGGATCCACAAATCATGAAAGGGAACAAGATGATGAAGATGAAGAGGAGCAGGATGCTACTCCGGAGTATAAACAGTTGATTGCGATTCACGCAGGGAAGCCGAAAGGTGGATTAGATATATGGCATATCGATCCAATTAAAGTCAGACGTCTTAGCTTGAGCGAGCAACAACTCGAAAATGAAACAAAAACTCGTGGATCCGACATTGTCAGGTTTACTCAGAGGAATTTGTTGAGGGTTTATCCAAAGGGTACACGTTTGGACTCATCAAATTATAATCCTATGCTTGGATGGAGTCATGGAGCTCAAATGGTGGCATTCAATATGCAAGGATATGGAAGGTACCTTTGGATTATGGAAGGAATGTTCAGAGCAAATGGAGGTTGTGGCTATGTGAAAAAACCTGAATTTTTGCTGGCTGTTGGCCCAAATAATGAGGTTTTTGACCCTGATGAACCTTTGTTACCAGTGAGAACAACTTTGAAGGTAAAAGTATACCTGGGTGAAGGATGGCATTCGGACTTCCACCATACACACTTCGATCTATATTCTCCACCGGACTTCTTCGTGAGGGTTGGAATTGCTGGAGTCCCAAGGGACACAAAAATGATGGAAACCGAACCAATAGAGGACCAATGGGTACCAGTGTGGAACAAGGAGTTCACCTTCCCATTGACGGTTCCAGACCTGGCTGTGCTTAGAGTTGAAGTCAAAGAGTATGACACTTCTGGGAATCATGACTTTGGTGGCCAAACATGTCTGCCAATCCGAGAGCTCAGAACTGGGATCCGAGCTGTTCCTCTGCACAACAAAAGAGGTCAAAAGTATAAATCTGTGAGGCTTCTGATGAGATTTGAATTTGACAATGAGGAGTAA

Protein Analysis

1147

Amino Acids

131.43

Weight (kDa)

6.22

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 115 - 257 1.1e-52 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 352 - 438 1.9e-28 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 460 - 562 9.8e-13 C2 domain
PI-PLC-X PF00388 699 - 841 1.2e-49 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 906 - 992 3.5e-29 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 1015 - 1116 1.4e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 554, 2841
AarI CACCTGC 1 cut(s) 205
AatII GACGTC 1 cut(s) 2716
Acc36I ACCTGC 3 cut(s) 205, 716, 1920
Acc65I GGTACC 2 cut(s) 2907, 3205
AccB1I GGYRCC 4 cut(s) 231, 1892, 2907, 3205
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 2 cut(s) 2179, 3060
AccII CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
AccIII TCCGGA 2 cut(s) 1833, 2626
AcoI YGGCCR 7 cut(s) 144, 379, 485, 1648, 2131, 2237, 3313
AcsI RAATTY 9 cut(s) 490, 1823, 1877, 2098, 2462, 2474, 2791, 2972, 3422
AcuI CTGAAG 1 cut(s) 1872
AcyI GRCGYC 1 cut(s) 2713
AflII CTTAAG 1 cut(s) 788
AflIII ACRYGT 3 cut(s) 1623, 2819, 3320
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 153, 336, 3063, 3252
AjuI GAANNNNNNNTTGG 4 cut(s) 2090, 2122, 2896, 2928
AloI GAACNNNNNNTCC 2 cut(s) 2915, 2947
Alw21I GWGCWC 5 cut(s) 153, 1210, 2088, 2872, 3342
Alw26I GTCTC 5 cut(s) 1672, 1672, 1991, 2024, 2542
Alw44I GTGCAC 1 cut(s) 2084
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 2 cut(s) 1076, 1583
Aor13HI TCCGGA 2 cut(s) 1833, 2626
AoxI GGCC 8 cut(s) 144, 379, 485, 1648, 2131, 2237, 2989, 3313
ApaLI GTGCAC 1 cut(s) 2084
ApeKI GCWGC 6 cut(s) 127, 130, 220, 423, 1085, 2200
ApoI RAATTY 9 cut(s) 490, 1823, 1877, 2098, 2462, 2474, 2791, 2972, 3422
ArsI GACNNNNNNTTYG 4 cut(s) 1671, 1703, 2321, 2353
Asp718I GGTACC 2 cut(s) 2907, 3205
AspLEI GCGC 7 cut(s) 135, 179, 261, 1024, 2200, 2319, 2321
AspS9I GGNCC 5 cut(s) 108, 528, 2299, 2990, 3196
AsuC2I CCSGG 1 cut(s) 2241
AsuHPI GGTGA 8 cut(s) 58, 326, 695, 1399, 2447, 2475, 3080, 3220
AsuII TTCGAA 1 cut(s) 87
AvaI CYCGRG 2 cut(s) 1076, 1583
AvaII GGWCC 4 cut(s) 108, 528, 2299, 3196
BaeGI GKGCMC 1 cut(s) 2088
BaeI ACNNNNGTAYC 6 cut(s) 1601, 1634, 2516, 2549, 3197, 3230
BalI TGGCCA 3 cut(s) 487, 1650, 3315
BamHI GGATCC 3 cut(s) 2567, 2762, 3351
BanI GGYRCC 4 cut(s) 231, 1892, 2907, 3205
BanII GRGCYC 3 cut(s) 153, 2872, 3342
BarI GAAGNNNNNNTAC 2 cut(s) 2367, 2399
BauI CACGAG 1 cut(s) 2757
BbsI GAAGAC 4 cut(s) 282, 630, 1382, 2382
Bbv12I GWGCWC 5 cut(s) 153, 1210, 2088, 2872, 3342
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 6 cut(s) 117, 139, 207, 435, 1072, 2187
BceAI ACGGC 2 cut(s) 135, 651
BcgI CGANNNNNNTGC 2 cut(s) 1067, 1101
BciT130I CCWGG 4 cut(s) 155, 338, 3065, 3254
BciVI GTATCC 1 cut(s) 1155
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 1 cut(s) 2241
BcoDI GTCTC 5 cut(s) 1672, 1672, 1991, 2024, 2542
BfaI CTAG 2 cut(s) 33, 1470
BfmI CTRYAG 3 cut(s) 221, 421, 1913
BfrI CTTAAG 1 cut(s) 788
BfuAI ACCTGC 3 cut(s) 205, 716, 1920
BfuI GTATCC 1 cut(s) 1155
BglI GCCNNNNNGGC 1 cut(s) 2408
BglII AGATCT 1 cut(s) 1244
BlpI GCTNAGC 1 cut(s) 1055
Bme1390I CCNGG 5 cut(s) 155, 338, 2241, 3065, 3254
Bme18I GGWCC 4 cut(s) 108, 528, 2299, 3196
BmeT110I CYCGRG 2 cut(s) 1076, 1583
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 5 cut(s) 108, 528, 2299, 2990, 3196
BmrFI CCNGG 5 cut(s) 155, 338, 2241, 3065, 3254
BmrI ACTGGG 2 cut(s) 2433, 3357
BmsI GCATC 7 cut(s) 654, 1188, 1248, 1954, 2002, 2020, 2608
BmuI ACTGGG 2 cut(s) 2433, 3357
BpiI GAAGAC 4 cut(s) 282, 630, 1382, 2382
BpmI CTGGAG 3 cut(s) 137, 759, 3171
Bpu10I CCTNAGC 1 cut(s) 99
Bpu1102I GCTNAGC 1 cut(s) 1055
Bpu14I TTCGAA 1 cut(s) 87
BpuEI CTTGAG 3 cut(s) 1081, 1973, 2743
BpuMI CCSGG 1 cut(s) 2241
Bsa29I ATCGAT 2 cut(s) 1905, 2694
BsaBI GATNNNNATC 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 2713
BsaI GGTCTC 1 cut(s) 2024
BsaJI CCNNGG 8 cut(s) 65, 154, 520, 1002, 1956, 2050, 3064, 3158
BsaWI WCCGGW 6 cut(s) 1833, 2293, 2485, 2527, 2626, 3121
BsaXI ACNNNNNCTCC 4 cut(s) 757, 787, 1544, 1574
Bse118I RCCGGY 3 cut(s) 377, 1492, 2129
Bse1I ACTGG 8 cut(s) 403, 1367, 1664, 1947, 2428, 3032, 3209, 3352
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 309
BseAI TCCGGA 2 cut(s) 1833, 2626
BseBI CCWGG 4 cut(s) 155, 338, 3065, 3254
BseCI ATCGAT 2 cut(s) 1905, 2694
BseDI CCNNGG 8 cut(s) 65, 154, 520, 1002, 1956, 2050, 3064, 3158
BseJI GATNNNNATC 1 cut(s) 309
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 7 cut(s) 90, 966, 1620, 1982, 2340, 2798, 3355
BseNI ACTGG 8 cut(s) 403, 1367, 1664, 1947, 2428, 3032, 3209, 3352
BsePI GCGCGC 1 cut(s) 2317
BseRI GAGGAG 8 cut(s) 794, 940, 1159, 2039, 2058, 2442, 2515, 2624
BseSI GKGCMC 1 cut(s) 2088
BseXI GCAGC 6 cut(s) 117, 139, 207, 435, 1072, 2187
BsgI GTGCAG 2 cut(s) 1713, 3353
Bsh1236I CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
BshFI GGCC 8 cut(s) 146, 381, 487, 1650, 2133, 2239, 2991, 3315
BshNI GGYRCC 4 cut(s) 231, 1892, 2907, 3205
BshVI ATCGAT 2 cut(s) 1905, 2694
BsiHKAI GWGCWC 5 cut(s) 153, 1210, 2088, 2872, 3342
BsiHKCI CYCGRG 2 cut(s) 1076, 1583
BslFI GGGAC 9 cut(s) 83, 121, 253, 343, 1682, 2293, 2440, 3140, 3176
BsmAI GTCTC 5 cut(s) 1672, 1672, 1991, 2024, 2542
BsmBI CGTCTC 1 cut(s) 2542
BsmFI GGGAC 9 cut(s) 83, 121, 253, 343, 1682, 2293, 2440, 3140, 3176
BsmI GAATGC 3 cut(s) 83, 2882, 3079
BsnI GGCC 8 cut(s) 146, 381, 487, 1650, 2133, 2239, 2991, 3315
Bso31I GGTCTC 1 cut(s) 2024
BsoBI CYCGRG 2 cut(s) 1076, 1583
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 5 cut(s) 153, 1210, 2088, 2872, 3342
Bsp13I TCCGGA 2 cut(s) 1833, 2626
Bsp1720I GCTNAGC 1 cut(s) 1055
BspANI GGCC 8 cut(s) 146, 381, 487, 1650, 2133, 2239, 2991, 3315
BspCNI CTCAG 7 cut(s) 91, 965, 1621, 1981, 2341, 2797, 3354
BspDI ATCGAT 2 cut(s) 1905, 2694
BspEI TCCGGA 2 cut(s) 1833, 2626
BspFNI CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
BspHI TCATGA 3 cut(s) 2536, 2578, 3301
BspMAI CTGCAG 3 cut(s) 225, 425, 1917
BspMI ACCTGC 3 cut(s) 205, 716, 1920
BspQI GCTCTTC 1 cut(s) 51
BspT104I TTCGAA 1 cut(s) 87
BspT107I GGYRCC 4 cut(s) 231, 1892, 2907, 3205
BspTI CTTAAG 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 2024
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 3 cut(s) 377, 1492, 2129
BsrI ACTGG 8 cut(s) 403, 1367, 1664, 1947, 2428, 3032, 3209, 3352
BssAI RCCGGY 3 cut(s) 377, 1492, 2129
BssECI CCNNGG 8 cut(s) 65, 154, 520, 1002, 1956, 2050, 3064, 3158
BssHII GCGCGC 1 cut(s) 2317
BssNAI GTATAC 2 cut(s) 2180, 3061
BssNI GRCGYC 1 cut(s) 2713
BssSI CACGAG 1 cut(s) 2757
BssT1I CCWWGG 3 cut(s) 1002, 1956, 3158
Bst1107I GTATAC 2 cut(s) 2180, 3061
Bst2BI CACGAG 1 cut(s) 2757
Bst2UI CCWGG 4 cut(s) 155, 338, 3065, 3254
Bst4CI ACNGT 6 cut(s) 905, 1579, 2174, 2260, 2640, 3244
Bst6I CTCTTC 5 cut(s) 51, 221, 1551, 2492, 2601
BstACI GRCGYC 1 cut(s) 2713
BstAFI CTTAAG 1 cut(s) 788
BstAPI GCANNNNNTGC 2 cut(s) 220, 989
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 5 cut(s) 582, 1494, 2319, 2729, 2982
BstDSI CCRYGG 2 cut(s) 65, 520
BstFNI CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
BstHHI GCGC 7 cut(s) 135, 179, 261, 1024, 2200, 2319, 2321
BstMAI GTCTC 5 cut(s) 1672, 1672, 1991, 2024, 2542
BstMWI GCNNNNNNNGC 8 cut(s) 220, 229, 656, 989, 1747, 2342, 2408, 2654
BstNI CCWGG 4 cut(s) 155, 338, 3065, 3254
BstNSI RCATGY 6 cut(s) 25, 515, 584, 1627, 2443, 3324
BstSCI CCNGG 5 cut(s) 153, 336, 2239, 3063, 3252
BstSFI CTRYAG 3 cut(s) 221, 421, 1913
BstSLI GKGCMC 1 cut(s) 2088
BstUI CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
BstV1I GCAGC 6 cut(s) 117, 139, 207, 435, 1072, 2187
BstV2I GAAGAC 4 cut(s) 282, 630, 1382, 2382
BstX2I RGATCY 4 cut(s) 1244, 2567, 2762, 3351
BstYI RGATCY 4 cut(s) 1244, 2567, 2762, 3351
BstZ17I GTATAC 2 cut(s) 2180, 3061
Bsu15I ATCGAT 2 cut(s) 1905, 2694
BsuI GTATCC 1 cut(s) 1155
BsuRI GGCC 8 cut(s) 146, 381, 487, 1650, 2133, 2239, 2991, 3315
BsuTUI ATCGAT 2 cut(s) 1905, 2694
BtgI CCRYGG 2 cut(s) 65, 520
BtrI CACGTC 1 cut(s) 73
BtsI GCAGTG 1 cut(s) 1922
BtsIMutI CAGTG 7 cut(s) 659, 910, 1922, 1954, 2421, 3039, 3216
BveI ACCTGC 3 cut(s) 205, 716, 1920
Cac8I GCNNGC 5 cut(s) 582, 1494, 2319, 2729, 2982
CaiI CAGNNNCTG 1 cut(s) 220
CciI TCATGA 3 cut(s) 2536, 2578, 3301
CfoI GCGC 7 cut(s) 135, 179, 261, 1024, 2200, 2319, 2321
Cfr10I RCCGGY 3 cut(s) 377, 1492, 2129
Cfr13I GGNCC 5 cut(s) 108, 528, 2299, 2990, 3196
Cfr42I CCGCGG 1 cut(s) 68
ClaI ATCGAT 2 cut(s) 1905, 2694
CseI GACGC 4 cut(s) 168, 203, 1028, 2290
CspCI CAANNNNNGTGG 4 cut(s) 525, 560, 2277, 2312
DraI TTTAAA 2 cut(s) 1014, 1383
EaeI YGGCCR 7 cut(s) 144, 379, 485, 1648, 2131, 2237, 3313
Eam1104I CTCTTC 5 cut(s) 51, 221, 1551, 2492, 2601
EarI CTCTTC 5 cut(s) 51, 221, 1551, 2492, 2601
EciI GGCGGA 1 cut(s) 137
Ecl136II GAGCTC 3 cut(s) 151, 2870, 3340
Eco130I CCWWGG 3 cut(s) 1002, 1956, 3158
Eco24I GRGCYC 3 cut(s) 153, 2872, 3342
Eco31I GGTCTC 1 cut(s) 2024
Eco32I GATATC 1 cut(s) 2045
Eco47I GGWCC 4 cut(s) 108, 528, 2299, 3196
Eco53kI GAGCTC 3 cut(s) 151, 2870, 3340
Eco57I CTGAAG 1 cut(s) 1872
Eco88I CYCGRG 2 cut(s) 1076, 1583
EcoICRI GAGCTC 3 cut(s) 151, 2870, 3340
EcoO109I RGGNCCY 2 cut(s) 108, 528
EcoRI GAATTC 3 cut(s) 2098, 2462, 2474
EcoRII CCWGG 4 cut(s) 153, 336, 3063, 3252
EcoRV GATATC 1 cut(s) 2045
EcoT14I CCWWGG 3 cut(s) 1002, 1956, 3158
EcoT22I ATGCAT 2 cut(s) 1203, 1263
EcoT38I GRGCYC 3 cut(s) 153, 2872, 3342
ErhI CCWWGG 3 cut(s) 1002, 1956, 3158
Esp3I CGTCTC 1 cut(s) 2542
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 9 cut(s) 83, 121, 253, 343, 1682, 2293, 2440, 3140, 3176
FauI CCCGC 1 cut(s) 60
FbaI TGATCA 1 cut(s) 249
FblI GTMKAC 2 cut(s) 2179, 3060
FriOI GRGCYC 3 cut(s) 153, 2872, 3342
FspBI CTAG 2 cut(s) 33, 1470
GlaI GCGC 7 cut(s) 134, 178, 260, 1023, 2199, 2318, 2320
GsuI CTGGAG 3 cut(s) 137, 759, 3171
HaeIII GGCC 8 cut(s) 146, 381, 487, 1650, 2133, 2239, 2991, 3315
HgaI GACGC 4 cut(s) 168, 203, 1028, 2290
HhaI GCGC 7 cut(s) 135, 179, 261, 1024, 2200, 2319, 2321
Hin1I GRCGYC 1 cut(s) 2713
Hin6I GCGC 7 cut(s) 133, 177, 259, 1022, 2198, 2317, 2319
HinP1I GCGC 7 cut(s) 133, 177, 259, 1022, 2198, 2317, 2319
HphI GGTGA 8 cut(s) 58, 326, 695, 1399, 2447, 2475, 3080, 3220
Hpy99I CGWCG 3 cut(s) 184, 197, 431
HpyCH4III ACNGT 6 cut(s) 905, 1579, 2174, 2260, 2640, 3244
HpyCH4IV ACGT 4 cut(s) 72, 429, 2713, 2821
HpyF10VI GCNNNNNNNGC 8 cut(s) 220, 229, 656, 989, 1747, 2342, 2408, 2654
HpySE526I ACGT 4 cut(s) 72, 429, 2713, 2821
Hsp92I GRCGYC 1 cut(s) 2713
HspAI GCGC 7 cut(s) 133, 177, 259, 1022, 2198, 2317, 2319
KflI GGGWCCC 1 cut(s) 108
Kpn2I TCCGGA 2 cut(s) 1833, 2626
KpnI GGTACC 2 cut(s) 2911, 3209
KroI GCCGGC 1 cut(s) 1492
KroNI GCCGGC 1 cut(s) 1494
Ksp22I TGATCA 1 cut(s) 249
KspI CCGCGG 1 cut(s) 68
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 9 cut(s) 124, 156, 199, 211, 355, 472, 1836, 2611, 2867
Lsp1109I GCAGC 6 cut(s) 117, 139, 207, 435, 1072, 2187
LweI GCATC 7 cut(s) 654, 1188, 1248, 1954, 2002, 2020, 2608
MaeI CTAG 2 cut(s) 33, 1470
MaeII ACGT 4 cut(s) 72, 429, 2713, 2821
MaeIII GTNAC 8 cut(s) 416, 723, 899, 2168, 2270, 2419, 2435, 3027
MbiI CCGCTC 1 cut(s) 202
MflI RGATCY 4 cut(s) 1244, 2567, 2762, 3351
MhlI GDGCHC 5 cut(s) 153, 1210, 2088, 2872, 3342
MlsI TGGCCA 3 cut(s) 487, 1650, 3315
MluNI TGGCCA 3 cut(s) 487, 1650, 3315
MlyI GAGTC 4 cut(s) 815, 2822, 2869, 3162
MmeI TCCRAC 3 cut(s) 1175, 2790, 3121
Mox20I TGGCCA 3 cut(s) 487, 1650, 3315
Mph1103I ATGCAT 2 cut(s) 1203, 1263
MroI TCCGGA 2 cut(s) 1833, 2626
MroNI GCCGGC 1 cut(s) 1492
MscI TGGCCA 3 cut(s) 487, 1650, 3315
MslI CAYNNNNRTG 5 cut(s) 831, 1546, 1617, 2028, 3170
Msp20I TGGCCA 3 cut(s) 487, 1650, 3315
MspA1I CMGCKG 2 cut(s) 67, 130
MspCI CTTAAG 1 cut(s) 788
MspR9I CCNGG 5 cut(s) 155, 338, 2241, 3065, 3254
Mva1269I GAATGC 3 cut(s) 83, 2882, 3079
MvaI CCWGG 4 cut(s) 155, 338, 3065, 3254
MvnI CGCG 7 cut(s) 67, 179, 197, 1022, 2198, 2319, 2321
MwoI GCNNNNNNNGC 8 cut(s) 220, 229, 656, 989, 1747, 2342, 2408, 2654
NaeI GCCGGC 1 cut(s) 1494
NciI CCSGG 1 cut(s) 2241
NgoMIV GCCGGC 1 cut(s) 1492
NmeAIII GCCGAG 2 cut(s) 172, 2031
NmuCI GTSAC 5 cut(s) 416, 899, 2270, 2419, 2435
NsiI ATGCAT 2 cut(s) 1203, 1263
NspI RCATGY 6 cut(s) 25, 515, 584, 1627, 2443, 3324
NspV TTCGAA 1 cut(s) 87
PaeI GCATGC 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 1076
PagI TCATGA 3 cut(s) 2536, 2578, 3301
PaqCI CACCTGC 1 cut(s) 205
PauI GCGCGC 1 cut(s) 2317
PciI ACATGT 2 cut(s) 1623, 3320
PciSI GCTCTTC 1 cut(s) 51
PctI GAATGC 3 cut(s) 83, 2882, 3079
PdiI GCCGGC 1 cut(s) 1494
PflFI GACNNNGTC 1 cut(s) 2771
PleI GAGTC 4 cut(s) 815, 2822, 2868, 3161
PpsI GAGTC 4 cut(s) 815, 2822, 2868, 3161
PpuMI RGGWCCY 2 cut(s) 108, 528
PscI ACATGT 2 cut(s) 1623, 3320
PsiI TTATAA 2 cut(s) 554, 2841
Psp124BI GAGCTC 3 cut(s) 153, 2872, 3342
Psp5II RGGWCCY 2 cut(s) 108, 528
Psp6I CCWGG 4 cut(s) 153, 336, 3063, 3252
PspGI CCWGG 4 cut(s) 153, 336, 3063, 3252
PspPI GGNCC 5 cut(s) 108, 528, 2299, 2990, 3196
PspPPI RGGWCCY 2 cut(s) 108, 528
PsrI GAACNNNNNNTAC 2 cut(s) 3012, 3044
PstI CTGCAG 3 cut(s) 225, 425, 1917
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 4 cut(s) 1244, 2567, 2762, 3351
PsyI GACNNNGTC 1 cut(s) 2771
PteI GCGCGC 1 cut(s) 2317
PvuII CAGCTG 1 cut(s) 130
RseI CAYNNNNRTG 5 cut(s) 831, 1546, 1617, 2028, 3170
SacI GAGCTC 3 cut(s) 153, 2872, 3342
SacII CCGCGG 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 51
Sau96I GGNCC 5 cut(s) 108, 528, 2299, 2990, 3196
SchI GAGTC 4 cut(s) 815, 2822, 2869, 3162
ScrFI CCNGG 5 cut(s) 155, 338, 2241, 3065, 3254
SduI GDGCHC 5 cut(s) 153, 1210, 2088, 2872, 3342
SfaNI GCATC 7 cut(s) 654, 1188, 1248, 1954, 2002, 2020, 2608
SfcI CTRYAG 3 cut(s) 221, 421, 1913
Sfr274I CTCGAG 1 cut(s) 1076
Sfr303I CCGCGG 1 cut(s) 68
SfuI TTCGAA 1 cut(s) 87
SgrBI CCGCGG 1 cut(s) 68
SinI GGWCC 4 cut(s) 108, 528, 2299, 3196
SlaI CTCGAG 1 cut(s) 1076
SmiMI CAYNNNNRTG 5 cut(s) 831, 1546, 1617, 2028, 3170
SmlI CTYRAG 5 cut(s) 788, 1060, 1076, 1988, 2722
SmoI CTYRAG 5 cut(s) 788, 1060, 1076, 1988, 2722
SphI GCATGC 1 cut(s) 584
SspMI CTAG 2 cut(s) 33, 1470
SstI GAGCTC 3 cut(s) 153, 2872, 3342
StyD4I CCNGG 5 cut(s) 153, 336, 2239, 3063, 3252
StyI CCWWGG 3 cut(s) 1002, 1956, 3158
TaaI ACNGT 6 cut(s) 905, 1579, 2174, 2260, 2640, 3244
TaiI ACGT 4 cut(s) 75, 432, 2716, 2824
TatI WGTACW 1 cut(s) 2445
TauI GCSGC 5 cut(s) 138, 174, 177, 1054, 1186
TscAI CASTG 7 cut(s) 666, 910, 1922, 1954, 2428, 3039, 3216
TseFI GTSAC 5 cut(s) 416, 899, 2270, 2419, 2435
TseI GCWGC 6 cut(s) 127, 130, 220, 423, 1085, 2200
Tsp45I GTSAC 5 cut(s) 416, 899, 2270, 2419, 2435
TspGWI ACGGA 4 cut(s) 342, 537, 2289, 2566
TspRI CASTG 7 cut(s) 666, 910, 1922, 1954, 2428, 3039, 3216
Tth111I GACNNNGTC 1 cut(s) 2771
Vha464I CTTAAG 1 cut(s) 788
VneI GTGCAC 1 cut(s) 2084
VpaK11BI GGWCC 4 cut(s) 108, 528, 2299, 3196
XapI RAATTY 9 cut(s) 490, 1823, 1877, 2098, 2462, 2474, 2791, 2972, 3422
XceI RCATGY 6 cut(s) 25, 515, 584, 1627, 2443, 3324
XhoI CTCGAG 1 cut(s) 1076
XmiI GTMKAC 2 cut(s) 2179, 3060
XspI CTAG 2 cut(s) 33, 1470
ZraI GACGTC 1 cut(s) 2714
Zsp2I ATGCAT 2 cut(s) 1203, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.