pycom03g07020

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007641
Physical Location & Seq
Forward (+)
5577187 .. 5579412
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGAGTCTTCTTCTATTCTTTCACTTTACCCTACTTAAAGTCCCCCCCCCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGGCTCTGCAACGTCGGTCAAGCGGATCTGCTTTGTGGATAAAGATGGAAAATCAGCTTCAGTATCACCAATATGAGTGCCTTCTGTTTGATCTAGATGACACCCTTTACCCTCTGAGTTCTGGAATTTCAGTGCAAATCACTACGAATATTAAAGAATATATGATTCAAAAGCTTGGCATGGACGAAAGTGAAGTTCCGGAATTGTGTATTTCTTTGTACAAGGATTATGGAACAACAATGGCTGGTCTTAAGGCGAAGGGCTATAACTTCGACTATGATGACTTTCATAGTTTTGTTCATGGGAGGTTGCCATATGAATTGCTTCATCCTGATCCTGTTCTGAGGGGTCTTTTACTTAGACTGCCTGTCCGGAAAGTCATTTTCACGAATTCGGATAAGGCACATGCGGCTACTGTGCTCAAAAGGCTTGGAATTGAGGACTGCTTTGAGTCGGTTATATGCTTTGAGACACTGAATCCCACCAACTGTGCTGATGATTCTGTGGGTGCAGGTGTAGCTGAACACATTGAACAACCAGATACTGGTGCGGTAATTCCAAAGACCCCAGTTGTCTCCAAACCATTTGAACAGGCATATGAAGAAGCTTTTAAGATAGCAAACATCGACCCTCAAAGAACGTTATTCTTCGACGATAGCATTCGGAATTTACAAACTGCAAAGCAGTGTGGCCTTCACACAGTATTGGTTGGCACCTCTCACCGAACTAAAGGCGTGGACCATGCACTTGAGAGCATCCATAATATGAAGGAAGCACTGCCAGAACTCTGGGAAACCAGCACCGGGGAGTCTGAAAATGTCCGGAATTCACGAGAGATTGCAATTGAGCAACTGGTAGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

325

Amino Acids

36.24

Weight (kDa)

5.22

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 62 - 267 1.5e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 653
AccIII TCCGGA 3 cut(s) 307, 480, 930
AciI CCGC 3 cut(s) 132, 518, 659
AclI AACGTT 1 cut(s) 749
AclWI GGATC 2 cut(s) 142, 437
AcsI RAATTY 4 cut(s) 234, 499, 775, 934
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 2 cut(s) 653, 912
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 3 cut(s) 278, 641, 698
AhdI GACNNNNNGTC 1 cut(s) 476
AluBI AGCT 5 cut(s) 166, 283, 629, 716, 971
AluI AGCT 5 cut(s) 166, 283, 629, 716, 971
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 2 cut(s) 572, 688
AlwI GGATC 2 cut(s) 142, 437
AlwNI CAGNNNCTG 1 cut(s) 653
Aor13HI TCCGGA 3 cut(s) 307, 480, 930
AoxI GGCC 1 cut(s) 799
ApoI RAATTY 4 cut(s) 234, 499, 775, 934
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 847
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 2 cut(s) 167, 821
AvaII GGWCC 1 cut(s) 847
BanI GGYRCC 1 cut(s) 821
BauI CACGAG 1 cut(s) 939
Bbv12I GWGCWC 1 cut(s) 531
BccI CCATC 1 cut(s) 148
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 572, 688
BfaI CTAG 1 cut(s) 203
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 611
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 1 cut(s) 913
Bme18I GGWCC 1 cut(s) 847
BmeRI GACNNNNNGTC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 847
BmiI GGNNCC 1 cut(s) 823
BmrFI CCNGG 1 cut(s) 913
BmrI ACTGGG 1 cut(s) 671
BmsI GCATC 1 cut(s) 873
BmuI ACTGGG 1 cut(s) 671
BpuEI CTTGAG 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 913
BsaJI CCNNGG 1 cut(s) 912
BsaWI WCCGGW 3 cut(s) 307, 480, 930
Bsc4I CCNNNNNNNGG 2 cut(s) 653, 912
Bse1I ACTGG 3 cut(s) 658, 677, 966
BseAI TCCGGA 3 cut(s) 307, 480, 930
BseDI CCNNGG 1 cut(s) 912
BseGI GGATG 2 cut(s) 436, 864
BseLI CCNNNNNNNGG 2 cut(s) 653, 912
BseMII CTCAG 2 cut(s) 215, 443
BseNI ACTGG 3 cut(s) 658, 677, 966
BseRI GAGGAG 1 cut(s) 42
BsgI GTGCAG 1 cut(s) 639
BshFI GGCC 1 cut(s) 801
BshNI GGYRCC 1 cut(s) 821
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 4 cut(s) 308, 481, 912, 931
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 653, 912
BsmAI GTCTC 2 cut(s) 572, 688
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 768
BsnI GGCC 1 cut(s) 801
Bsp1286I GDGCHC 1 cut(s) 531
Bsp13I TCCGGA 3 cut(s) 307, 480, 930
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 3 cut(s) 134, 199, 442
BspACI CCGC 3 cut(s) 132, 518, 659
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 2 cut(s) 216, 444
BspEI TCCGGA 3 cut(s) 307, 480, 930
BspLI GGNNCC 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 2 cut(s) 142, 437
BspT107I GGYRCC 1 cut(s) 821
BspTI CTTAAG 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 3 cut(s) 658, 677, 966
BssECI CCNNGG 1 cut(s) 912
BssMI GATC 3 cut(s) 134, 199, 442
BssSI CACGAG 1 cut(s) 939
Bst2BI CACGAG 1 cut(s) 939
Bst4CI ACNGT 3 cut(s) 526, 599, 811
BstAFI CTTAAG 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 327
BstDEI CTNAG 4 cut(s) 224, 452, 467, 972
BstF5I GGATG 2 cut(s) 436, 864
BstKTI GATC 3 cut(s) 137, 202, 445
BstMAI GTCTC 2 cut(s) 572, 688
BstMBI GATC 3 cut(s) 134, 199, 442
BstMWI GCNNNNNNNGC 3 cut(s) 518, 535, 626
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 911
BstX2I RGATCY 1 cut(s) 134
BstXI CCANNNNNNTGG 1 cut(s) 897
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 1 cut(s) 801
BtsCI GGATG 2 cut(s) 436, 864
BtsI GCAGTG 2 cut(s) 800, 884
BtsIMutI CAGTG 4 cut(s) 246, 581, 800, 884
BveI ACCTGC 1 cut(s) 611
CaiI CAGNNNCTG 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 847
Csp6I GTAC 1 cut(s) 328
CviAII CATG 4 cut(s) 289, 410, 515, 851
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 4 cut(s) 224, 452, 467, 972
DpnI GATC 3 cut(s) 136, 201, 444
DpnII GATC 3 cut(s) 134, 199, 442
DriI GACNNNNNGTC 1 cut(s) 476
Eam1105I GACNNNNNGTC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 152
EcoRI GAATTC 2 cut(s) 499, 934
FaeI CATG 4 cut(s) 292, 413, 518, 854
FaqI GGGAC 1 cut(s) 26
FatI CATG 4 cut(s) 288, 409, 514, 850
FauNDI CATATG 2 cut(s) 424, 706
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 2 cut(s) 423, 851
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 1 cut(s) 203
GluI GCNGC 1 cut(s) 519
HaeIII GGCC 1 cut(s) 801
HapII CCGG 4 cut(s) 308, 481, 912, 931
Hin1II CATG 4 cut(s) 292, 413, 518, 854
HindIII AAGCTT 3 cut(s) 281, 714, 969
HinfI GANTC 6 cut(s) 4, 274, 560, 586, 608, 917
HpaII CCGG 4 cut(s) 308, 481, 912, 931
HphI GGTGA 2 cut(s) 167, 821
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 5 cut(s) 225, 453, 505, 774, 922
Hpy188III TCNNGA 8 cut(s) 203, 231, 308, 440, 481, 496, 931, 939
Hpy8I GTNNAC 1 cut(s) 847
Hpy99I CGWCG 2 cut(s) 126, 764
HpyAV CCTTC 4 cut(s) 200, 361, 812, 871
HpyCH4III ACNGT 3 cut(s) 526, 599, 811
HpyCH4IV ACGT 2 cut(s) 121, 749
HpyCH4V TGCA 6 cut(s) 118, 244, 620, 788, 854, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 518, 535, 626
HpyF3I CTNAG 4 cut(s) 224, 452, 467, 972
HpySE526I ACGT 2 cut(s) 121, 749
Hsp92II CATG 4 cut(s) 292, 413, 518, 854
Kpn2I TCCGGA 3 cut(s) 307, 480, 930
Kzo9I GATC 3 cut(s) 134, 199, 442
LweI GCATC 1 cut(s) 873
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 121, 749
MalI GATC 3 cut(s) 136, 201, 444
MboI GATC 3 cut(s) 134, 199, 442
MboII GAAGA 2 cut(s) 722, 748
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 9 cut(s) 234, 311, 428, 499, 543, 663, 775, 934, 951
MlyI GAGTC 3 cut(s) 13, 569, 926
MnlI CCTC 8 cut(s) 60, 63, 231, 408, 447, 541, 750, 835
MroI TCCGGA 3 cut(s) 307, 480, 930
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 4 cut(s) 36, 261, 360, 720
MslI CAYNNNNRTG 1 cut(s) 180
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 4 cut(s) 308, 481, 912, 931
MspR9I CCNGG 1 cut(s) 913
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 1 cut(s) 768
MwoI GCNNNNNNNGC 3 cut(s) 518, 535, 626
NciI CCSGG 1 cut(s) 913
NdeI CATATG 2 cut(s) 424, 706
NdeII GATC 3 cut(s) 134, 199, 442
NlaIII CATG 4 cut(s) 292, 413, 518, 854
NlaIV GGNNCC 1 cut(s) 823
NspI RCATGY 1 cut(s) 518
PaqCI CACCTGC 1 cut(s) 611
PctI GAATGC 1 cut(s) 768
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 3 cut(s) 274, 586, 608
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 1 cut(s) 520
PleI GAGTC 3 cut(s) 12, 568, 925
PpsI GAGTC 3 cut(s) 12, 568, 925
Psp1406I AACGTT 1 cut(s) 749
PspN4I GGNNCC 1 cut(s) 823
PspPI GGNCC 1 cut(s) 847
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 4 cut(s) 36, 261, 360, 720
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 3 cut(s) 134, 199, 442
Sau96I GGNCC 1 cut(s) 847
SchI GAGTC 3 cut(s) 13, 569, 926
ScrFI CCNGG 1 cut(s) 913
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 1 cut(s) 873
SinI GGWCC 1 cut(s) 847
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 359, 857
SmoI CTYRAG 2 cut(s) 359, 857
Sse9I AATT 9 cut(s) 234, 311, 428, 499, 543, 663, 775, 934, 951
SsiI CCGC 3 cut(s) 132, 518, 659
SspI AATATT 1 cut(s) 259
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 911
TaaI ACNGT 3 cut(s) 526, 599, 811
TaiI ACGT 2 cut(s) 124, 752
TaqI TCGA 3 cut(s) 381, 735, 759
TaqII GACCGA 1 cut(s) 114
TasI AATT 9 cut(s) 234, 311, 428, 499, 543, 663, 775, 934, 951
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 3 cut(s) 274, 586, 608
Tru1I TTAA 4 cut(s) 36, 261, 360, 720
Tru9I TTAA 4 cut(s) 36, 261, 360, 720
TscAI CASTG 4 cut(s) 246, 588, 800, 891
TspDTI ATGAA 6 cut(s) 386, 398, 425, 441, 723, 890
TspRI CASTG 4 cut(s) 246, 588, 800, 891
Van91I CCANNNNNTGG 1 cut(s) 653
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 4 cut(s) 234, 499, 775, 934
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 518
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.