Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0013053

Physical Location & Seq

Genomic Coordinates Reverse (-)

5940224 .. 5942906

Gene Structure 2683 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGGTTAAAAGCCCGCCACTGAAAGCCTTCTCAATCTTCAACTCCTCCATCCTCCGCGGCTTCCATCACACCCATCAATCCATTTCCTTCATTCTCCACCAGCTCCTCGCCTCCAACTTGATCTCCCACGCCCAGTCCCTCTTCCTTCAAGTCCTCTCCGGCCGCCTTTCCTCACCGTTCTTCACTCCCTCTTCCCTCCTCGGGCACTTAACTCAGCCCAATCTGAATCCAACCTCCGTCCGCGGCCATCTATACGAAGCAATAGTCAATGCCCATGTTCAATCTCAGTCTCCGGAGCAAGCCCTTTACTTTTTGAAGCAGATGGTTGAGCAGGGCCTCGTTCCCAGATCGAACACTTTCAATAATCTGTTGGGTTTTCTCGTGAAGGCAAAAGATCACGGCAAAGCGTGGCGGGTTTTCGATGAGTTTAAGGGCAAAGTTGAATTGGATGTGTATAGTTTTGGGATAGTGATGAAAAGTTGCTGCGAGGCCGGCAATTTGGATCGGGGATTTGAGCTTTTGATTGAATTAGAGGAATTGGGTTGGGCGCCGAATGTTGTTTTGTACACTACATTGATAGATGGATGCTGCAAAAATGGCGATTTGGAGCGTGCCAAGAAGATGTTTTGTAAAATAGAGGAGGTTGGTTTGGTTGCTAACCAGTATACTTACACTGTTTTGATTCATGGGTTTTTCAAGAAAGGGTATAAAAAAGATGGGTTCGAGCTCTACGAGAAGATGAAAAGTAATGGGGTTGTTCCGAATGTGTGTACTTATACTTGTTTGATCGATGAGTGTTGCAGTAATGGGGAAGTGAAGAGAGCCCTGAAACTGTTCGACGAAATGCGTGAAAGAGGGGTGGCTCGCAATGTGGTAGCGTACAACACCGTGATCGGTGGGTTGTGCAAGGAAATGAGGATTTGGGAAGCTGATAACTTTGTGAATCGGATGAAGAGGGAGGGTATTAGTCCGAATGTAGTTACGTACAATGCGCTGATTGAGGGGTTTTGCAACGTCGGAGAGTTGGACAGGGCTTCGAGTTTATTTAATCAGTTGAAGTCAAGTGGACAGTCTCCAACTTTGGTGACTTACAATGTTTTGATTCAAGGCTTTGCTAGAGCACAAAATTCTGCAAGAGTTTCTGATTTGGTGAGAGAGATGAGCGACAGAGGCGTTTCTCCTTCAAAAGTGACATACACAATCTTGATCAATGGCTTGGTTCGATCTAGTGACACAGAAAGAGCATTTCAGATATTCTCTTCCATGGAGAAGGCCGGAGTGGTGCCCGATGTTTACACCTACGGTGTCTTGATTCATGGGTCATGCACAAAAGGTGATATGAAGGAAGCATCGAAGCTGTTCAAATCAATGAGTGACAAGCATTTGAATCCAAATGATGTCATATTCAATATGATGATTCATGGTTACTGCGGAGAAGGTAGCTCTTACAGGGCACTGAGGCTGCTCAAAGAGATGAGAAGGAACGGAATGATTCCGAATGTGGCCAGCTACAGTTCGACCATTGGAGTTCTCTGTAATGATGGGAAGTGGGAGGAGGCTGAAATGCTGCTCAAGGACATGACAGAGTCAGATGCTGTCGGCTACTCGCGCTTTCCACCGCGTGTGTTGCACGCGCCAGCACACGGACCGGCCTCTGGGTCTGTCCATTTTGGGCTGGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0050896 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1900864 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

562

Amino Acids

62.45

Weight (kDa)

8.51

Isoelectric Point (pI)

33.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	78 - 173	7.8e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	117 - 162	5.5e-07	PPR repeat family
PPR_long	PF17177	145 - 262	3.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	183 - 211	1.5e-10	PPR repeat
PPR_2	PF13041	184 - 233	1.8e-15	PPR repeat family
PPR	PF01535	187 - 216	5.4e-08	PPR repeat
PPR_long	PF17177	191 - 305	6e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	216 - 248	9.6e-06	PPR repeat
PPR_3	PF13812	217 - 266	1.3e-09	Pentatricopeptide repeat domain
PPR	PF01535	222 - 252	3.8e-06	PPR repeat
PPR_2	PF13041	225 - 267	5.3e-13	PPR repeat family
PPR_1	PF12854	250 - 283	3.1e-13	PPR repeat
PPR_2	PF13041	254 - 290	2.8e-11	PPR repeat family
TPR_24	PF23276	258 - 351	3.4e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	258 - 299	1.4e-06	Pentatricopeptide repeat domain
PPR	PF01535	258 - 287	6.6e-09	PPR repeat
PPR_long	PF17177	260 - 376	5.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	285 - 318	3.7e-07	PPR repeat
PPR_2	PF13041	289 - 338	2.7e-17	PPR repeat family
PPR_3	PF13812	315 - 369	1.2e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	321 - 352	2.7e-12	PPR repeat
PPR_2	PF13041	324 - 373	3.4e-17	PPR repeat family
PPR	PF01535	327 - 356	1.8e-06	PPR repeat
PPR_2	PF13041	362 - 408	3.4e-13	PPR repeat family
PPR_long	PF17177	378 - 465	1.4e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	383 - 434	9.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	390 - 423	3.6e-07	PPR repeat
PPR	PF01535	397 - 427	3.6e-06	PPR repeat
PPR_2	PF13041	397 - 442	1e-14	PPR repeat family
PPR_3	PF13812	422 - 476	2e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	426 - 457	1.7e-10	PPR repeat
PPR_1	PF12854	460 - 493	3.7e-07	PPR repeat
PPR_2	PF13041	464 - 513	1.7e-12	PPR repeat family
PPR	PF01535	467 - 496	5e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11690 AT4G11690
fragaria_vesca	FvH4_3g36170
malus_domestica	MD03G1092900.v1.1
prunus_persica	Prupe.6G076800_v2.0.a1
pyrus_communis	pycom03g07420
rosa_chinensis	RchiOBHm_Chr5g0064671 RchiOBHm_Chr5g0064911
rosa_laevigata	RLG00000035740 RLG00000035742
rosa_multiflora	Rmu_sc0020903.1_g000003 Rmu_sc0025431.1_g000001
rosa_roxburghii	Rroxscaffold_1G00016400
rosa_rugosa	Rorug05G0366600
rosa_samantha	Rh5AG425100 Rh5CG462600 Rh5DG453100
rosa_wichuraiana	Rw5G039940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	547, 1282
AccI	GTMKAC	1 cut(s)	665
AccII	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
AccIII	TCCGGA	1 cut(s)	292
AciI	CCGC	9 cut(s)	14, 55, 57, 163, 241, 243, 412, 1431, 1619
AclWI	GGATC	1 cut(s)	510
AcoI	YGGCCR	3 cut(s)	160, 244, 1503
AcsI	RAATTY	1 cut(s)	1126
AcyI	GRCGYC	1 cut(s)	548
AdeI	CACNNNGTG	1 cut(s)	1622
AfaI	GTAC	4 cut(s)	566, 772, 881, 986
AfiI	CCNNNNNNNGG	3 cut(s)	201, 1643, 1655
AluBI	AGCT	7 cut(s)	103, 517, 727, 929, 1357, 1443, 1509
AluI	AGCT	7 cut(s)	103, 517, 727, 929, 1357, 1443, 1509
Alw21I	GWGCWC	2 cut(s)	729, 1123
Alw26I	GTCTC	2 cut(s)	294, 1077
AlwI	GGATC	1 cut(s)	510
AlwNI	CAGNNNCTG	1 cut(s)	1595
Ama87I	CYCGRG	1 cut(s)	200
Aor13HI	TCCGGA	1 cut(s)	292
AoxI	GGCC	7 cut(s)	160, 244, 334, 489, 1272, 1503, 1650
ApeKI	GCWGC	4 cut(s)	483, 588, 1462, 1567
ApoI	RAATTY	1 cut(s)	1126
ArsI	GACNNNNNNTTYG	2 cut(s)	249, 281
Asp700I	GAANNNNTTC	4 cut(s)	26, 356, 833, 1358
AspLEI	GCGC	4 cut(s)	550, 994, 1611, 1636
AspS9I	GGNCC	2 cut(s)	334, 1646
AsuHPI	GGTGA	4 cut(s)	165, 1096, 1162, 1346
AvaI	CYCGRG	1 cut(s)	200
AvaII	GGWCC	1 cut(s)	1646
BaeGI	GKGCMC	3 cut(s)	207, 1287, 1456
BalI	TGGCCA	1 cut(s)	1505
BanI	GGYRCC	2 cut(s)	547, 1282
BanII	GRGCYC	2 cut(s)	729, 826
BauI	CACGAG	1 cut(s)	380
Bbv12I	GWGCWC	2 cut(s)	729, 1123
BbvI	GCAGC	4 cut(s)	470, 575, 1449, 1554
BccI	CCATC	8 cut(s)	56, 72, 81, 255, 316, 575, 710, 1535
BceAI	ACGGC	1 cut(s)	415
BclI	TGATCA	1 cut(s)	1206
BcoDI	GTCTC	2 cut(s)	294, 1077
BfaI	CTAG	2 cut(s)	1116, 1227
BfmI	CTRYAG	1 cut(s)	1510
BfoI	RGCGCY	1 cut(s)	551
BisI	GCNGC	7 cut(s)	58, 163, 244, 484, 589, 1463, 1568
BlsI	GCNGC	7 cut(s)	59, 164, 245, 485, 590, 1464, 1569
Bme18I	GGWCC	1 cut(s)	1646
BmeT110I	CYCGRG	1 cut(s)	200
BmgT120I	GGNCC	2 cut(s)	334, 1646
BmiI	GGNNCC	2 cut(s)	549, 1284
BmrI	ACTGGG	1 cut(s)	127
BmsI	GCATC	3 cut(s)	575, 1358, 1582
BmuI	ACTGGG	1 cut(s)	127
BpuEI	CTTGAG	1 cut(s)	1556
Bsa29I	ATCGAT	1 cut(s)	789
BsaAI	YACGTR	1 cut(s)	984
BsaHI	GRCGYC	1 cut(s)	548
BsaJI	CCNNGG	4 cut(s)	55, 199, 241, 1263
BsaWI	WCCGGW	1 cut(s)	292
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bsc4I	CCNNNNNNNGG	3 cut(s)	201, 1643, 1655
Bse118I	RCCGGY	2 cut(s)	491, 1648
Bse1I	ACTGG	2 cut(s)	133, 661
Bse3DI	GCAATG	1 cut(s)	874
BseAI	TCCGGA	1 cut(s)	292
BseCI	ATCGAT	1 cut(s)	789
BseDI	CCNNGG	4 cut(s)	55, 199, 241, 1263
BseGI	GGATG	4 cut(s)	48, 454, 590, 954
BseLI	CCNNNNNNNGG	3 cut(s)	201, 1643, 1655
BseMI	GCAATG	1 cut(s)	874
BseMII	CTCAG	3 cut(s)	227, 299, 1448
BseNI	ACTGG	2 cut(s)	133, 661
BseRI	GAGGAG	5 cut(s)	34, 95, 188, 653, 1568
BseSI	GKGCMC	3 cut(s)	207, 1287, 1456
BseX3I	CGGCCG	1 cut(s)	160
BseXI	GCAGC	4 cut(s)	470, 575, 1449, 1554
Bsh1236I	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
Bsh1285I	CGRYCG	1 cut(s)	163
BshFI	GGCC	7 cut(s)	162, 246, 336, 491, 1274, 1505, 1652
BshNI	GGYRCC	2 cut(s)	547, 1282
BshVI	ATCGAT	1 cut(s)	789
BsiEI	CGRYCG	1 cut(s)	163
BsiHKAI	GWGCWC	2 cut(s)	729, 1123
BsiHKCI	CYCGRG	1 cut(s)	200
BsiSI	CCGG	5 cut(s)	159, 293, 492, 1275, 1649
BslFI	GGGAC	1 cut(s)	121
BslI	CCNNNNNNNGG	3 cut(s)	201, 1643, 1655
BsmAI	GTCTC	2 cut(s)	294, 1077
BsmFI	GGGAC	1 cut(s)	121
BsnI	GGCC	7 cut(s)	162, 246, 336, 491, 1274, 1505, 1652
BsoBI	CYCGRG	1 cut(s)	200
Bsp1286I	GDGCHC	6 cut(s)	207, 729, 826, 1123, 1287, 1456
Bsp13I	TCCGGA	1 cut(s)	292
Bsp1407I	TGTACA	1 cut(s)	564
Bsp143I	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
Bsp19I	CCATGG	1 cut(s)	1263
BspACI	CCGC	9 cut(s)	14, 55, 57, 163, 241, 243, 412, 1431, 1619
BspANI	GGCC	7 cut(s)	162, 246, 336, 491, 1274, 1505, 1652
BspCNI	CTCAG	3 cut(s)	226, 298, 1449
BspDI	ATCGAT	1 cut(s)	789
BspEI	TCCGGA	1 cut(s)	292
BspFNI	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
BspLI	GGNNCC	2 cut(s)	549, 1284
BspPI	GGATC	1 cut(s)	510
BspT107I	GGYRCC	2 cut(s)	547, 1282
BsrDI	GCAATG	1 cut(s)	874
BsrFI	RCCGGY	2 cut(s)	491, 1648
BsrGI	TGTACA	1 cut(s)	564
BsrI	ACTGG	2 cut(s)	133, 661
BssAI	RCCGGY	2 cut(s)	491, 1648
BssECI	CCNNGG	4 cut(s)	55, 199, 241, 1263
BssMI	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
BssNAI	GTATAC	1 cut(s)	666
BssNI	GRCGYC	1 cut(s)	548
BssSI	CACGAG	1 cut(s)	380
BssT1I	CCWWGG	1 cut(s)	1263
Bst1107I	GTATAC	1 cut(s)	666
Bst2BI	CACGAG	1 cut(s)	380
Bst4CI	ACNGT	7 cut(s)	177, 676, 834, 889, 1071, 1304, 1514
Bst6I	CTCTTC	5 cut(s)	146, 196, 812, 947, 1264
BstACI	GRCGYC	1 cut(s)	548
BstAUI	TGTACA	1 cut(s)	564
BstBAI	YACGTR	1 cut(s)	984
BstC8I	GCNNGC	9 cut(s)	14, 300, 493, 612, 865, 1507, 1632, 1638, 1677
BstDEI	CTNAG	3 cut(s)	213, 285, 1457
BstDSI	CCRYGG	3 cut(s)	55, 241, 1263
BstF5I	GGATG	4 cut(s)	48, 454, 590, 954
BstFNI	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
BstH2I	RGCGCY	1 cut(s)	551
BstHHI	GCGC	4 cut(s)	550, 994, 1611, 1636
BstKTI	GATC	8 cut(s)	123, 350, 397, 505, 789, 894, 1209, 1226
BstMAI	GTCTC	2 cut(s)	294, 1077
BstMBI	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
BstMCI	CGRYCG	1 cut(s)	163
BstMWI	GCNNNNNNNGC	5 cut(s)	492, 597, 1170, 1608, 1627
BstSFI	CTRYAG	1 cut(s)	1510
BstSLI	GKGCMC	3 cut(s)	207, 1287, 1456
BstSNI	TACGTA	1 cut(s)	984
BstUI	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
BstV1I	GCAGC	4 cut(s)	470, 575, 1449, 1554
BstZ17I	GTATAC	1 cut(s)	666
BstZI	CGGCCG	1 cut(s)	160
Bsu15I	ATCGAT	1 cut(s)	789
BsuRI	GGCC	7 cut(s)	162, 246, 336, 491, 1274, 1505, 1652
BsuTUI	ATCGAT	1 cut(s)	789
BtgI	CCRYGG	3 cut(s)	55, 241, 1263
BtsCI	GGATG	4 cut(s)	48, 454, 590, 954
BtsIMutI	CAGTG	3 cut(s)	17, 672, 1454
Cac8I	GCNNGC	9 cut(s)	14, 300, 493, 612, 865, 1507, 1632, 1638, 1677
CaiI	CAGNNNCTG	1 cut(s)	1595
CfoI	GCGC	4 cut(s)	550, 994, 1611, 1636
Cfr10I	RCCGGY	2 cut(s)	491, 1648
Cfr13I	GGNCC	2 cut(s)	334, 1646
Cfr42I	CCGCGG	2 cut(s)	58, 244
ClaI	ATCGAT	1 cut(s)	789
CpoI	CGGWCCG	1 cut(s)	1646
Csp6I	GTAC	4 cut(s)	565, 771, 880, 985
CspI	CGGWCCG	1 cut(s)	1646
CviAII	CATG	7 cut(s)	275, 686, 1264, 1316, 1323, 1421, 1579
CviQI	GTAC	4 cut(s)	565, 771, 880, 985
DdeI	CTNAG	3 cut(s)	213, 285, 1457
DinI	GGCGCC	1 cut(s)	549
DpnI	GATC	8 cut(s)	122, 349, 396, 504, 788, 893, 1208, 1225
DpnII	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
DraIII	CACNNNGTG	1 cut(s)	1622
EaeI	YGGCCR	3 cut(s)	160, 244, 1503
EagI	CGGCCG	1 cut(s)	160
Eam1104I	CTCTTC	5 cut(s)	146, 196, 812, 947, 1264
EarI	CTCTTC	5 cut(s)	146, 196, 812, 947, 1264
Ecl136II	GAGCTC	1 cut(s)	727
EclXI	CGGCCG	1 cut(s)	160
Eco105I	TACGTA	1 cut(s)	984
Eco130I	CCWWGG	1 cut(s)	1263
Eco24I	GRGCYC	2 cut(s)	729, 826
Eco47I	GGWCC	1 cut(s)	1646
Eco52I	CGGCCG	1 cut(s)	160
Eco53kI	GAGCTC	1 cut(s)	727
Eco88I	CYCGRG	1 cut(s)	200
EcoICRI	GAGCTC	1 cut(s)	727
EcoO109I	RGGNCCY	1 cut(s)	334
EcoT14I	CCWWGG	1 cut(s)	1263
EcoT38I	GRGCYC	2 cut(s)	729, 826
EgeI	GGCGCC	1 cut(s)	549
EheI	GGCGCC	1 cut(s)	549
ErhI	CCWWGG	1 cut(s)	1263
FaeI	CATG	7 cut(s)	278, 689, 1267, 1319, 1326, 1424, 1582
FaqI	GGGAC	1 cut(s)	121
FatI	CATG	7 cut(s)	274, 685, 1263, 1315, 1322, 1420, 1578
FauI	CCCGC	2 cut(s)	21, 405
FbaI	TGATCA	1 cut(s)	1206
FblI	GTMKAC	1 cut(s)	665
Fnu4HI	GCNGC	7 cut(s)	58, 163, 244, 484, 589, 1463, 1568
FokI	GGATG	4 cut(s)	35, 461, 597, 961
FriOI	GRGCYC	2 cut(s)	729, 826
Fsp4HI	GCNGC	7 cut(s)	58, 163, 244, 484, 589, 1463, 1568
FspBI	CTAG	2 cut(s)	1116, 1227
GlaI	GCGC	4 cut(s)	549, 993, 1610, 1635
GluI	GCNGC	7 cut(s)	58, 163, 244, 484, 589, 1463, 1568
HaeII	RGCGCY	1 cut(s)	551
HaeIII	GGCC	7 cut(s)	162, 246, 336, 491, 1274, 1505, 1652
HapII	CCGG	5 cut(s)	159, 293, 492, 1275, 1649
HhaI	GCGC	4 cut(s)	550, 994, 1611, 1636
Hin1I	GRCGYC	1 cut(s)	548
Hin1II	CATG	7 cut(s)	278, 689, 1267, 1319, 1326, 1424, 1582
Hin6I	GCGC	4 cut(s)	548, 992, 1609, 1634
HinP1I	GCGC	4 cut(s)	548, 992, 1609, 1634
HinfI	GANTC	9 cut(s)	226, 682, 943, 1102, 1312, 1387, 1417, 1492, 1586
HpaII	CCGG	5 cut(s)	159, 293, 492, 1275, 1649
HphI	GGTGA	4 cut(s)	165, 1096, 1162, 1346
Hpy166II	GTNNAC	5 cut(s)	567, 666, 771, 1067, 1294
Hpy188I	TCNGA	9 cut(s)	225, 762, 948, 972, 1019, 1144, 1251, 1497, 1591
Hpy188III	TCNNGA	6 cut(s)	293, 382, 697, 1204, 1309, 1683
Hpy8I	GTNNAC	5 cut(s)	567, 666, 771, 1067, 1294
Hpy99I	CGWCG	2 cut(s)	842, 1019
HpyAV	CCTTC	9 cut(s)	37, 97, 155, 379, 1191, 1264, 1336, 1430, 1473
HpyCH4III	ACNGT	7 cut(s)	177, 676, 834, 889, 1071, 1304, 1514
HpyCH4IV	ACGT	2 cut(s)	983, 1014
HpyCH4V	TGCA	7 cut(s)	591, 801, 906, 1011, 1133, 1326, 1630
HpyF10VI	GCNNNNNNNGC	5 cut(s)	492, 597, 1170, 1608, 1627
HpyF3I	CTNAG	3 cut(s)	213, 285, 1457
HpySE526I	ACGT	2 cut(s)	983, 1014
Hsp92I	GRCGYC	1 cut(s)	548
Hsp92II	CATG	7 cut(s)	278, 689, 1267, 1319, 1326, 1424, 1582
HspAI	GCGC	4 cut(s)	548, 992, 1609, 1634
KasI	GGCGCC	1 cut(s)	547
Kpn2I	TCCGGA	1 cut(s)	292
KroI	GCCGGC	1 cut(s)	491
KroNI	GCCGGC	1 cut(s)	493
Ksp22I	TGATCA	1 cut(s)	1206
KspI	CCGCGG	2 cut(s)	58, 244
Kzo9I	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
LmnI	GCTCC	3 cut(s)	108, 295, 607
Lsp1109I	GCAGC	4 cut(s)	470, 575, 1449, 1554
LweI	GCATC	3 cut(s)	575, 1358, 1582
MaeI	CTAG	2 cut(s)	1116, 1227
MaeII	ACGT	2 cut(s)	983, 1014
MaeIII	GTNAC	6 cut(s)	979, 1084, 1189, 1229, 1373, 1424
MalI	GATC	8 cut(s)	122, 349, 396, 504, 788, 893, 1208, 1225
MboI	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
MboII	GAAGA	9 cut(s)	28, 133, 172, 183, 631, 748, 829, 964, 1251
MhlI	GDGCHC	6 cut(s)	207, 729, 826, 1123, 1287, 1456
MlsI	TGGCCA	1 cut(s)	1505
MluCI	AATT	5 cut(s)	443, 496, 527, 536, 1126
MluNI	TGGCCA	1 cut(s)	1505
Mly113I	GGCGCC	1 cut(s)	548
MlyI	GAGTC	1 cut(s)	1595
MmeI	TCCRAC	5 cut(s)	138, 254, 997, 1005, 1100
Mox20I	TGGCCA	1 cut(s)	1505
MroI	TCCGGA	1 cut(s)	292
MroNI	GCCGGC	1 cut(s)	491
MroXI	GAANNNNTTC	4 cut(s)	26, 356, 833, 1358
MscI	TGGCCA	1 cut(s)	1505
MseI	TTAA	4 cut(s)	6, 209, 429, 1047
Msp20I	TGGCCA	1 cut(s)	1505
MspA1I	CMGCKG	2 cut(s)	57, 243
MspI	CCGG	5 cut(s)	159, 293, 492, 1275, 1649
MvnI	CGCG	5 cut(s)	57, 243, 1609, 1621, 1634
MwoI	GCNNNNNNNGC	5 cut(s)	492, 597, 1170, 1608, 1627
NaeI	GCCGGC	1 cut(s)	493
NarI	GGCGCC	1 cut(s)	548
NcoI	CCATGG	1 cut(s)	1263
NdeII	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
NgoMIV	GCCGGC	1 cut(s)	491
NlaIII	CATG	7 cut(s)	278, 689, 1267, 1319, 1326, 1424, 1582
NlaIV	GGNNCC	2 cut(s)	549, 1284
NmuCI	GTSAC	4 cut(s)	1084, 1189, 1229, 1373
PcsI	WCGNNNNNNNCGW	1 cut(s)	729
PdiI	GCCGGC	1 cut(s)	493
PdmI	GAANNNNTTC	4 cut(s)	26, 356, 833, 1358
PfeI	GAWTC	8 cut(s)	226, 682, 943, 1102, 1312, 1387, 1417, 1492
PflFI	GACNNNGTC	1 cut(s)	1585
PkrI	GCNGC	7 cut(s)	59, 164, 245, 485, 590, 1464, 1569
PleI	GAGTC	1 cut(s)	1594
PluTI	GGCGCC	1 cut(s)	551
PpsI	GAGTC	1 cut(s)	1594
Ppu21I	YACGTR	1 cut(s)	984
Psp124BI	GAGCTC	1 cut(s)	729
PspN4I	GGNNCC	2 cut(s)	549, 1284
PspPI	GGNCC	2 cut(s)	334, 1646
PstNI	CAGNNNCTG	1 cut(s)	1595
PsyI	GACNNNGTC	1 cut(s)	1585
RsaI	GTAC	4 cut(s)	566, 772, 881, 986
RsaNI	GTAC	4 cut(s)	565, 771, 880, 985
Rsr2I	CGGWCCG	1 cut(s)	1646
RsrII	CGGWCCG	1 cut(s)	1646
SacI	GAGCTC	1 cut(s)	729
SacII	CCGCGG	2 cut(s)	58, 244
SaqAI	TTAA	4 cut(s)	6, 209, 429, 1047
SatI	GCNGC	7 cut(s)	58, 163, 244, 484, 589, 1463, 1568
Sau3AI	GATC	8 cut(s)	120, 347, 394, 502, 786, 891, 1206, 1223
Sau96I	GGNCC	2 cut(s)	334, 1646
SchI	GAGTC	1 cut(s)	1595
SduI	GDGCHC	6 cut(s)	207, 729, 826, 1123, 1287, 1456
SfaNI	GCATC	3 cut(s)	575, 1358, 1582
SfcI	CTRYAG	1 cut(s)	1510
SfoI	GGCGCC	1 cut(s)	549
Sfr303I	CCGCGG	2 cut(s)	58, 244
SgrBI	CCGCGG	2 cut(s)	58, 244
SinI	GGWCC	1 cut(s)	1646
SmlI	CTYRAG	1 cut(s)	1571
SmoI	CTYRAG	1 cut(s)	1571
SnaBI	TACGTA	1 cut(s)	984
Sse9I	AATT	5 cut(s)	443, 496, 527, 536, 1126
SsiI	CCGC	9 cut(s)	14, 55, 57, 163, 241, 243, 412, 1431, 1619
SspDI	GGCGCC	1 cut(s)	547
SspMI	CTAG	2 cut(s)	1116, 1227
SstI	GAGCTC	1 cut(s)	729
StyI	CCWWGG	1 cut(s)	1263
TaaI	ACNGT	7 cut(s)	177, 676, 834, 889, 1071, 1304, 1514
TaiI	ACGT	2 cut(s)	986, 1017
TaqI	TCGA	9 cut(s)	350, 420, 723, 789, 837, 1037, 1222, 1352, 1517
TasI	AATT	5 cut(s)	443, 496, 527, 536, 1126
TatI	WGTACW	2 cut(s)	564, 770
TauI	GCSGC	3 cut(s)	60, 165, 246
TfiI	GAWTC	8 cut(s)	226, 682, 943, 1102, 1312, 1387, 1417, 1492
Tru1I	TTAA	4 cut(s)	6, 209, 429, 1047
Tru9I	TTAA	4 cut(s)	6, 209, 429, 1047
TscAI	CASTG	3 cut(s)	24, 679, 1461
TseFI	GTSAC	4 cut(s)	1084, 1189, 1229, 1373
TseI	GCWGC	4 cut(s)	483, 588, 1462, 1567
Tsp45I	GTSAC	4 cut(s)	1084, 1189, 1229, 1373
TspDTI	ATGAA	8 cut(s)	79, 488, 674, 755, 965, 1304, 1355, 1409
TspGWI	ACGGA	3 cut(s)	226, 1500, 1659
TspRI	CASTG	3 cut(s)	24, 679, 1461
Tth111I	GACNNNGTC	1 cut(s)	1585
VpaK11BI	GGWCC	1 cut(s)	1646
XapI	RAATTY	1 cut(s)	1126
XmiI	GTMKAC	1 cut(s)	665
XmnI	GAANNNNTTC	4 cut(s)	26, 356, 833, 1358
XspI	CTAG	2 cut(s)	1116, 1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.