pycom03g09340

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007496
Physical Location & Seq
Reverse (-)
7844046 .. 7847115
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2574 bp
ATGAGTACATTGCGCTCTACATGTGAAATTGTTGAATCAAGAGAAGAGCTCCACTCGGAGTCCAAAATGGACGAGAAAGGTGCAAAACACAGCATGCTAAAATTAGGAAATAAATATTCAATCGAGGATGATATTAATCAGCTTTTTCAGGCAATTGATGGTAGAAAGTCTGCTAGGATATCAGGTTTGTCGCATGAGGCTAGCAAAGACGCTTTGAGGAAGAGTGCTATGAAAAGGCCAATGAGAGTTGGTTCATCAAATACATCAGGTATCGGAATTTCAGAGCCTGTGAGTTTGAAGCAAGCACTTAGAGGATTGTGCATCACTCAGGCATCAGAGTTGGCTGCCATGAAGCGGCTACCGAGGCTAGGTCGTTCATCAGGAGTTTCAGAAGCAGGAACTATCAAAAGGTTGTATGGCACAGTGGTAGCCGAGGCCAATGGGCCTGGCATACCTATGACTGAAGGTAAGGGGAATTATGTAGAGGTGTCCCTTGTGCCTGAAACATCCAAAAATTCTGAAAACAATCCTGGATCCATGCAATCAGAGACACAGGTTGATACTGCCAGAACTTTGGGAGCTGGACTGATGCCCGATGCTAGTAAGACCAGATTACCACCGCATGATCAAATTACTCCTTTTTCATCAGCAGTTAAAAGTGATATATCGAAGGGAGAAGATAGAGAACCGGAATCAGCAGAATCTTCATTCCATTTTCATCATGGTAAGAAAGTCATGCAGAGGGGTGCGGTTGCTTCTATCTCCACTGATGTTCCAATTAATTCTTCAAATTCAAACAAGGGGCTGCACTCTGCTGCTTCTCCATCTGGCTCTAGCACTGGTAGTAAGGTAGGCAAATCATCTGGCAGTAGTTCACATCTAATCAAGCCGGTTCTCAGGAGCAAAAACGTGGTTAAGAAAACTGTCAAGCAGGATTCAAGTTCTGGTTCTAGTAGTTCCAAATCAGATAACCGACAAGTTGATAGTGATTCGTGCTCTAGTACTAGCAAGTTAAAGGACCAGACACACAACTGTACTCCAAAGCATGAAGGGAAAGGAAATGAGAAATTATCTCTGGCATCAAGCAGTACAAGTCTTAGCAAAGAATTGAATTCAGGTATTGTTAACAGAGGTACAAGCACACTGGATTTCGCTTCGAATTACAGTAGTAGGATTAACTCTGCAGTGACAAAAGTTGATGAGAGGTCAAGATCAAAGGAAAACGGGGATTTCTCACAAAGCTCAAAAAGTAGCATTGGTGACGGTAGCAGCACTACATGCACTAGTGAGGAGAGCAGTCTAAGTGGGTCTGGTCGTAGCGGCAAGAGGCCACACATGTCAAGAGACTTGAGATGGGAAGCCATCCACCACGTCCAGAAGCAGCATGGAAGCTTAGGTTTGAAGCACTTTAAGTTGCTTAGGAGAATCGGTTCTGGGGACATTGGGACTGTTTATCTTGCTGAGCTAGCTGGAACAAATTGTGTATTTGCTTTGAAAGTGATGGACAACGAGTTTTTGGCTACCAGGAAGAAAATGATCAGGGCCCAAACAGAAAGAGAGATACTGGAAATGCTGGATCATCCATTTCTTCCCACACTATATTCCCATTGTGTGTCAGATAACCTCTCATGTTTGGTTATGGAGTATTGTCCGGGTGGGGATCTGCACGTGCTGCGGCAAAAGCAACCTGGCAGGAGTTTCTGTGAACAAGCAGCCAGGTTTTATGTTGCTGAAGTCCTCCTTGCCTTGGAGTATTTACACATGCTAGGAGTTGTGTATCGAGACTTGAAACCTGAAAATATTCTGGTCCGAGAAGACGGCCACATCATGCTCACAGATTTTGATTTGTCACTTAGATGTGCTGTCAATCCAACGCTGCTTAAATCATCGTCTCCTGTTTTGGAGAGCACAGGAAAAATGTCAAGCCCATGCATAGAATCTAGCTGCATTGATCCTTTTTGCCTTCAACCATCCTGGCAAGTTCCATGCTTCACTCCCAGACTTTTATCCGCCGCTGCAAAATCTCGAAAAGTGAAATCCGAGCTTGCTGCCCAGGTCAGCCCATTGCCACAGCTTGTAGTGGAGCCTACTAGTGCCCGGTCCAACTCATTTGTTGGAACCCATGAATACCTTGCCCCGGAGATCATTAAAGGAGACGGCCATGGGAATGCTGTTGATTGGTGGACATATGGAATCTTTCTGTTTGAGCTGTTATATGGTAAGACACCCTTCAAGGGGTCAGGAAATGAGGACACATTATCCAATGTTGTGTCGAAAAGTCTCAAGTTCCCAAATACACCTATAGTGAGTTTTTATGCAAGAGATCTGATCAGAGGCCTCTTAATAAAGGACCCTGAAAATCGTCTAGGATCAGTGAAAGGGGCTGCAGAAATCAAGCAGCATCCTTTCTTTGAAGGCCTCAATTGGGCATTGATTCGGTGTGCAGTACCGCCAGAGCTTCCCAAGTGGGGTGATATTGGAATTGGTACACCATCTGCTTCACAGAAACAGGAGAGCACGGGCTGCAAGGAGTTGGAGGGTATAGAAGAGCATATAGACTTTGACCTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

858

Amino Acids

92.97

Weight (kDa)

8.68

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 471 - 620 1.2e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 471 - 620 4.8e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 701 - 804 4.9e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 708 - 788 8.8e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 355, 620, 749, 1320, 1675, 2010, 2013, 2450
AclWI GGATC 6 cut(s) 528, 541, 1584, 1668, 1946, 2377
AcoI YGGCCR 2 cut(s) 1819, 2158
AcsI RAATTY 4 cut(s) 276, 514, 790, 1111
AcuI CTGAAG 2 cut(s) 483, 1752
AcvI CACGTG 1 cut(s) 1669
AfaI GTAC 7 cut(s) 7, 1003, 1036, 1090, 1135, 2448, 2488
AfiI CCNNNNNNNGG 8 cut(s) 354, 368, 1747, 2137, 2234, 2235, 2425, 2468
AflIII ACRYGT 2 cut(s) 20, 1335
AhlI ACTAGT 2 cut(s) 1283, 2090
AjiI CACGTC 1 cut(s) 1372
AjnI CCWGG 7 cut(s) 445, 529, 1523, 1687, 1715, 1973, 2052
AjuI GAANNNNNNNTTGG 2 cut(s) 769, 801
AloI GAACNNNNNNTCC 2 cut(s) 1414, 1446
Alw21I GWGCWC 4 cut(s) 51, 998, 1910, 2519
Alw26I GTCTC 6 cut(s) 542, 1338, 1776, 1896, 2148, 2285
AlwI GGATC 6 cut(s) 528, 541, 1584, 1668, 1946, 2377
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 9 cut(s) 236, 435, 443, 1328, 1542, 1819, 2158, 2335, 2416
ApaI GGGCCC 1 cut(s) 1546
ApoI RAATTY 4 cut(s) 276, 514, 790, 1111
AseI ATTAAT 2 cut(s) 135, 780
Asp700I GAANNNNTTC 2 cut(s) 1429, 1800
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 7 cut(s) 443, 1018, 1542, 1543, 1807, 2100, 2350
AsuC2I CCSGG 3 cut(s) 1653, 2098, 2138
AsuHPI GGTGA 2 cut(s) 1271, 2483
AsuII TTCGAA 1 cut(s) 1157
AsuNHI GCTAGC 2 cut(s) 200, 1465
AvaII GGWCC 4 cut(s) 1018, 1807, 2100, 2350
BaeGI GKGCMC 2 cut(s) 1546, 2098
BamHI GGATCC 1 cut(s) 533
BanII GRGCYC 2 cut(s) 51, 1546
BbrPI CACGTG 1 cut(s) 1669
BbsI GAAGAC 1 cut(s) 1821
Bbv12I GWGCWC 4 cut(s) 51, 998, 1910, 2519
BccI CCATC 7 cut(s) 152, 832, 1347, 1370, 1495, 1978, 2500
BceAI ACGGC 2 cut(s) 1834, 2173
BcgI CGANNNNNNTGC 4 cut(s) 62, 96, 2030, 2064
BciT130I CCWGG 7 cut(s) 447, 531, 1525, 1689, 1717, 1975, 2054
BclI TGATCA 3 cut(s) 625, 1536, 2328
BcnI CCSGG 3 cut(s) 1653, 2098, 2138
BcoDI GTCTC 6 cut(s) 542, 1338, 1776, 1896, 2148, 2285
BcuI ACTAGT 2 cut(s) 1283, 2090
BfmI CTRYAG 3 cut(s) 1182, 2301, 2385
BglII AGATCT 1 cut(s) 2323
BlpI GCTNAGC 1 cut(s) 1461
BmcAI AGTACT 1 cut(s) 1003
Bme18I GGWCC 4 cut(s) 1018, 1807, 2100, 2350
BmgBI CACGTC 1 cut(s) 1372
BmgT120I GGNCC 7 cut(s) 443, 1018, 1542, 1543, 1807, 2100, 2350
BmiI GGNNCC 5 cut(s) 535, 1544, 2085, 2119, 2352
BmsI GCATC 6 cut(s) 330, 341, 579, 586, 1088, 2410
BmtI GCTAGC 2 cut(s) 204, 1469
BpiI GAAGAC 1 cut(s) 1821
BplI GAGNNNNNCTC 4 cut(s) 33, 65, 38, 70
Bpu10I CCTNAGC 2 cut(s) 1393, 1418
Bpu1102I GCTNAGC 1 cut(s) 1461
Bpu14I TTCGAA 1 cut(s) 1157
BpuEI CTTGAG 2 cut(s) 1369, 2267
BpuMI CCSGG 3 cut(s) 1653, 2098, 2138
BsaAI YACGTR 1 cut(s) 1669
BsaBI GATNNNNATC 1 cut(s) 135
BsaJI CCNNGG 6 cut(s) 362, 432, 1746, 2052, 2136, 2161
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 4 cut(s) 1414, 1444, 1687, 1717
Bsc4I CCNNNNNNNGG 8 cut(s) 354, 368, 1747, 2137, 2234, 2235, 2425, 2468
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 3 cut(s) 844, 1149, 1569
Bse3DI GCAATG 2 cut(s) 8, 2063
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 7 cut(s) 447, 531, 1525, 1689, 1717, 1975, 2054
BseDI CCNNGG 6 cut(s) 362, 432, 1746, 2052, 2136, 2161
BseGI GGATG 6 cut(s) 133, 506, 1362, 1579, 1970, 2401
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 8 cut(s) 354, 368, 1747, 2137, 2234, 2235, 2425, 2468
BseMI GCAATG 2 cut(s) 8, 2063
BseMII CTCAG 3 cut(s) 341, 910, 1452
BseNI ACTGG 3 cut(s) 844, 1149, 1569
BseRI GAGGAG 1 cut(s) 1304
BseSI GKGCMC 2 cut(s) 1546, 2098
BsgI GTGCAG 3 cut(s) 791, 1649, 2463
BshFI GGCC 9 cut(s) 238, 437, 445, 1330, 1544, 1821, 2160, 2337, 2418
BsiHKAI GWGCWC 4 cut(s) 51, 998, 1910, 2519
BsiSI CCGG 5 cut(s) 689, 890, 1652, 2098, 2138
BslFI GGGAC 3 cut(s) 475, 1451, 1459
BslI CCNNNNNNNGG 8 cut(s) 354, 368, 1747, 2137, 2234, 2235, 2425, 2468
BsmAI GTCTC 6 cut(s) 542, 1338, 1776, 1896, 2148, 2285
BsmBI CGTCTC 2 cut(s) 1896, 2148
BsmFI GGGAC 3 cut(s) 475, 1451, 1459
BsmI GAATGC 1 cut(s) 2173
BsnI GGCC 9 cut(s) 238, 437, 445, 1330, 1544, 1821, 2160, 2337, 2418
Bsp119I TTCGAA 1 cut(s) 1157
Bsp120I GGGCCC 1 cut(s) 1542
Bsp1286I GDGCHC 6 cut(s) 51, 998, 1546, 1910, 2098, 2519
Bsp1720I GCTNAGC 1 cut(s) 1461
Bsp19I CCATGG 1 cut(s) 2161
BspACI CCGC 8 cut(s) 355, 620, 749, 1320, 1675, 2010, 2013, 2450
BspANI GGCC 9 cut(s) 238, 437, 445, 1330, 1544, 1821, 2160, 2337, 2418
BspCNI CTCAG 3 cut(s) 340, 909, 1453
BspLI GGNNCC 5 cut(s) 535, 1544, 2085, 2119, 2352
BspMAI CTGCAG 2 cut(s) 1186, 2389
BspOI GCTAGC 2 cut(s) 204, 1469
BspPI GGATC 6 cut(s) 528, 541, 1584, 1668, 1946, 2377
BspQI GCTCTTC 2 cut(s) 39, 2541
BspT104I TTCGAA 1 cut(s) 1157
BsrDI GCAATG 2 cut(s) 8, 2063
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 3 cut(s) 844, 1149, 1569
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 6 cut(s) 362, 432, 1746, 2052, 2136, 2161
BssT1I CCWWGG 2 cut(s) 1746, 2161
Bst2UI CCWGG 7 cut(s) 447, 531, 1525, 1689, 1717, 1975, 2054
Bst4CI ACNGT 6 cut(s) 424, 925, 1034, 1166, 1265, 1450
Bst6I CTCTTC 3 cut(s) 39, 215, 2541
BstAPI GCANNNNNTGC 3 cut(s) 1278, 1672, 2523
BstBAI YACGTR 1 cut(s) 1669
BstBI TTCGAA 1 cut(s) 1157
BstC8I GCNNGC 5 cut(s) 95, 202, 303, 1467, 2046
BstDEI CTNAG 9 cut(s) 308, 327, 896, 1097, 1301, 1393, 1418, 1461, 1853
BstDSI CCRYGG 1 cut(s) 2161
BstF5I GGATG 6 cut(s) 133, 506, 1362, 1579, 1970, 2401
BstHHI GCGC 1 cut(s) 15
BstMAI GTCTC 6 cut(s) 542, 1338, 1776, 1896, 2148, 2285
BstMWI GCNNNNNNNGC 6 cut(s) 364, 1278, 1466, 1672, 1681, 2523
BstNI CCWGG 7 cut(s) 447, 531, 1525, 1689, 1717, 1975, 2054
BstNSI RCATGY 5 cut(s) 24, 97, 1281, 1339, 1765
BstSFI CTRYAG 3 cut(s) 1182, 2301, 2385
BstSLI GKGCMC 2 cut(s) 1546, 2098
BstV2I GAAGAC 1 cut(s) 1821
BstX2I RGATCY 3 cut(s) 533, 1660, 2323
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 3 cut(s) 533, 1660, 2323
BsuRI GGCC 9 cut(s) 238, 437, 445, 1330, 1544, 1821, 2160, 2337, 2418
BtgI CCRYGG 1 cut(s) 2161
BtrI CACGTC 1 cut(s) 1372
BtsCI GGATG 6 cut(s) 133, 506, 1362, 1579, 1970, 2401
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 6 cut(s) 429, 765, 837, 1142, 1191, 2379
Cac8I GCNNGC 5 cut(s) 95, 202, 303, 1467, 2046
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 7 cut(s) 443, 1018, 1542, 1543, 1807, 2100, 2350
CseI GACGC 1 cut(s) 218
Csp6I GTAC 7 cut(s) 6, 1002, 1035, 1089, 1134, 2447, 2487
CviQI GTAC 7 cut(s) 6, 1002, 1035, 1089, 1134, 2447, 2487
DdeI CTNAG 9 cut(s) 308, 327, 896, 1097, 1301, 1393, 1418, 1461, 1853
EaeI YGGCCR 2 cut(s) 1819, 2158
Eam1104I CTCTTC 3 cut(s) 39, 215, 2541
EarI CTCTTC 3 cut(s) 39, 215, 2541
EciI GGCGGA 1 cut(s) 1999
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 1746, 2161
Eco147I AGGCCT 2 cut(s) 2337, 2418
Eco24I GRGCYC 2 cut(s) 51, 1546
Eco32I GATATC 1 cut(s) 180
Eco47I GGWCC 4 cut(s) 1018, 1807, 2100, 2350
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 483, 1752
Eco72I CACGTG 1 cut(s) 1669
EcoICRI GAGCTC 1 cut(s) 49
EcoO109I RGGNCCY 2 cut(s) 1542, 2350
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 7 cut(s) 445, 529, 1523, 1687, 1715, 1973, 2052
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 2 cut(s) 1746, 2161
EcoT22I ATGCAT 1 cut(s) 1934
EcoT38I GRGCYC 2 cut(s) 51, 1546
ErhI CCWWGG 2 cut(s) 1746, 2161
Esp3I CGTCTC 2 cut(s) 1896, 2148
FalI AAGNNNNNCTT 4 cut(s) 1725, 1757, 2213, 2245
FaqI GGGAC 3 cut(s) 475, 1451, 1459
FauNDI CATATG 1 cut(s) 2188
FbaI TGATCA 3 cut(s) 625, 1536, 2328
FokI GGATG 6 cut(s) 140, 493, 1349, 1566, 1957, 2388
FriOI GRGCYC 2 cut(s) 51, 1546
GlaI GCGC 1 cut(s) 14
HaeIII GGCC 9 cut(s) 238, 437, 445, 1330, 1544, 1821, 2160, 2337, 2418
HapII CCGG 5 cut(s) 689, 890, 1652, 2098, 2138
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 15
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 1390
HpaI GTTAAC 1 cut(s) 1126
HpaII CCGG 5 cut(s) 689, 890, 1652, 2098, 2138
HphI GGTGA 2 cut(s) 1271, 2483
Hpy166II GTNNAC 5 cut(s) 875, 1126, 1706, 2184, 2489
Hpy188III TCNNGA 9 cut(s) 39, 381, 898, 1209, 1341, 1375, 1781, 2025, 2241
Hpy8I GTNNAC 5 cut(s) 875, 1126, 1706, 2184, 2489
HpyAV CCTTC 6 cut(s) 458, 664, 1043, 1973, 2239, 2408
HpyCH4III ACNGT 6 cut(s) 424, 925, 1034, 1166, 1265, 1450
HpyCH4IV ACGT 3 cut(s) 909, 1371, 1668
HpyF10VI GCNNNNNNNGC 6 cut(s) 364, 1278, 1466, 1672, 1681, 2523
HpyF3I CTNAG 9 cut(s) 308, 327, 896, 1097, 1301, 1393, 1418, 1461, 1853
HpySE526I ACGT 3 cut(s) 909, 1371, 1668
HspAI GCGC 1 cut(s) 13
Ksp22I TGATCA 3 cut(s) 625, 1536, 2328
KspAI GTTAAC 1 cut(s) 1126
LguI GCTCTTC 2 cut(s) 39, 2541
LmnI GCTCC 4 cut(s) 54, 578, 900, 2083
LweI GCATC 6 cut(s) 330, 341, 579, 586, 1088, 2410
MaeII ACGT 3 cut(s) 909, 1371, 1668
MaeIII GTNAC 3 cut(s) 1186, 1259, 1848
MboII GAAGA 9 cut(s) 56, 232, 689, 696, 777, 1540, 1580, 1826, 2558
MfeI CAATTG 2 cut(s) 153, 2422
MflI RGATCY 3 cut(s) 533, 1660, 2323
MhlI GDGCHC 6 cut(s) 51, 998, 1546, 1910, 2098, 2519
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 4 cut(s) 1895, 2095, 2127, 2514
Mph1103I ATGCAT 1 cut(s) 1934
MroXI GAANNNNTTC 2 cut(s) 1429, 1800
MslI CAYNNNNRTG 3 cut(s) 455, 1855, 2166
MspA1I CMGCKG 1 cut(s) 2015
MspI CCGG 5 cut(s) 689, 890, 1652, 2098, 2138
MunI CAATTG 2 cut(s) 153, 2422
Mva1269I GAATGC 1 cut(s) 2173
MvaI CCWGG 7 cut(s) 447, 531, 1525, 1689, 1717, 1975, 2054
MwoI GCNNNNNNNGC 6 cut(s) 364, 1278, 1466, 1672, 1681, 2523
NciI CCSGG 3 cut(s) 1653, 2098, 2138
NcoI CCATGG 1 cut(s) 2161
NdeI CATATG 1 cut(s) 2188
NheI GCTAGC 2 cut(s) 200, 1465
NlaIV GGNNCC 5 cut(s) 535, 1544, 2085, 2119, 2352
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 3 cut(s) 1186, 1259, 1848
NsiI ATGCAT 1 cut(s) 1934
NspI RCATGY 5 cut(s) 24, 97, 1281, 1339, 1765
NspV TTCGAA 1 cut(s) 1157
PaeI GCATGC 1 cut(s) 97
PceI AGGCCT 2 cut(s) 2337, 2418
PciI ACATGT 2 cut(s) 20, 1335
PciSI GCTCTTC 2 cut(s) 39, 2541
PctI GAATGC 1 cut(s) 2173
PdmI GAANNNNTTC 2 cut(s) 1429, 1800
PfeI GAWTC 9 cut(s) 35, 692, 701, 935, 989, 1425, 1937, 2193, 2434
PfoI TCCNGGA 1 cut(s) 529
PleI GAGTC 1 cut(s) 67
PmaCI CACGTG 1 cut(s) 1669
PmlI CACGTG 1 cut(s) 1669
PpsI GAGTC 1 cut(s) 67
Ppu21I YACGTR 1 cut(s) 1669
PpuMI RGGWCCY 1 cut(s) 2350
PscI ACATGT 2 cut(s) 20, 1335
PshBI ATTAAT 2 cut(s) 135, 780
Psp124BI GAGCTC 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 2350
Psp6I CCWGG 7 cut(s) 445, 529, 1523, 1687, 1715, 1973, 2052
PspCI CACGTG 1 cut(s) 1669
PspGI CCWGG 7 cut(s) 445, 529, 1523, 1687, 1715, 1973, 2052
PspN4I GGNNCC 5 cut(s) 535, 1544, 2085, 2119, 2352
PspOMI GGGCCC 1 cut(s) 1542
PspPI GGNCC 7 cut(s) 443, 1018, 1542, 1543, 1807, 2100, 2350
PspPPI RGGWCCY 1 cut(s) 2350
PstI CTGCAG 2 cut(s) 1186, 2389
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 3 cut(s) 533, 1660, 2323
RsaI GTAC 7 cut(s) 7, 1003, 1036, 1090, 1135, 2448, 2488
RsaNI GTAC 7 cut(s) 6, 1002, 1035, 1089, 1134, 2447, 2487
RseI CAYNNNNRTG 3 cut(s) 455, 1855, 2166
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 2 cut(s) 39, 2541
Sau96I GGNCC 7 cut(s) 443, 1018, 1542, 1543, 1807, 2100, 2350
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 6 cut(s) 51, 998, 1546, 1910, 2098, 2519
SfaNI GCATC 6 cut(s) 330, 341, 579, 586, 1088, 2410
SfcI CTRYAG 3 cut(s) 1182, 2301, 2385
SfuI TTCGAA 1 cut(s) 1157
SinI GGWCC 4 cut(s) 1018, 1807, 2100, 2350
SmiMI CAYNNNNRTG 3 cut(s) 455, 1855, 2166
SmlI CTYRAG 2 cut(s) 1348, 2282
SmoI CTYRAG 2 cut(s) 1348, 2282
SpeI ACTAGT 2 cut(s) 1283, 2090
SphI GCATGC 1 cut(s) 97
SseBI AGGCCT 2 cut(s) 2337, 2418
SsiI CCGC 8 cut(s) 355, 620, 749, 1320, 1675, 2010, 2013, 2450
SspI AATATT 2 cut(s) 116, 1801
SstI GAGCTC 1 cut(s) 51
StuI AGGCCT 2 cut(s) 2337, 2418
StyI CCWWGG 2 cut(s) 1746, 2161
TaaI ACNGT 6 cut(s) 424, 925, 1034, 1166, 1265, 1450
TaiI ACGT 3 cut(s) 912, 1374, 1671
TaqI TCGA 6 cut(s) 123, 668, 1157, 1780, 2026, 2273
TatI WGTACW 4 cut(s) 5, 1001, 1034, 1088
TauI GCSGC 4 cut(s) 358, 1323, 1678, 2015
TfiI GAWTC 9 cut(s) 35, 692, 701, 935, 989, 1425, 1937, 2193, 2434
TscAI CASTG 6 cut(s) 429, 772, 844, 1149, 1191, 2379
TseFI GTSAC 3 cut(s) 1186, 1259, 1848
Tsp45I GTSAC 3 cut(s) 1186, 1259, 1848
TspDTI ATGAA 9 cut(s) 243, 245, 365, 366, 633, 696, 707, 1062, 2139
TspRI CASTG 6 cut(s) 429, 772, 844, 1149, 1191, 2379
VpaK11BI GGWCC 4 cut(s) 1018, 1807, 2100, 2350
VspI ATTAAT 2 cut(s) 135, 780
XapI RAATTY 4 cut(s) 276, 514, 790, 1111
XceI RCATGY 5 cut(s) 24, 97, 1281, 1339, 1765
XcmI CCANNNNNNNNNTGG 2 cut(s) 719, 1382
XmnI GAANNNNTTC 2 cut(s) 1429, 1800
ZrmI AGTACT 1 cut(s) 1003
Zsp2I ATGCAT 1 cut(s) 1934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.