Rw5G027130

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007496
Physical Location & Seq
Forward (+)
39847510 .. 39851318
3809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027130.1

Sequence Viewer

Length: 2457 bp
ATGAGTACATCAGGCATTACATGTGAGATTGTCGAAGCAGGAGAAGAAGTAGATTCTCAATTGAAAATGGATGAGAAAGATGGCAAGCAATGTGTGCCAAACTCAGGAAATAGGTATTCGATAGAGGATGATATTAACAAGCTTTTTCAGGCCATTGATGTTAGAAATTCAGGCAGGGCTTCAGGCCCATGGCATGAAAGTAGCAAAGATGCCTTGAGGAAGAGTGCTATGAAAAAGCCAATGAGAGCTAGTACATCAAAAGTATCAGGTATAGGAATTTCAGAGCCTGTAAGTTTGAAGCAAGCGCTCAGAGGATTATGCATCTCTCAGGCATCAGAGATGGCTGCCATGAAGCGGCTATCGAGGCCAGGTCGTTCATCTGGGGTTTCAGAAGCAGGAACTATTAAAAGGTTGTACAGGGCAGTGGTAGTCGAGGAAAATGGATCTGACCTCCCAATGAATGAAGGTAAAGGGAATTTTGTAGAGATCTCCTTAGTACCAGATGCATCAAATAGCTCTCAGCAGATGCCTGGATTCATGCAATCAGAGCCGCAGTTTCATCCGGCCACAACTTCAGCTGATGCAATGATGACCAAATTAGCACCACAGAAACAAATTTCTTCTTCGCCAACAGCAGTTCAGAGTAAGATATTTAAGGGAGAAGATGGAAAACTGGAAGATGCAGATACTTCATCAAGCAAAAAAGTCATACAGGTGGAGGTTCCAATCACTACTCTAAATTCGTACAAGGGTCAGCACTCTGCTTCTTCTCTATCTGGCAGTAAGTTAAGGAAATCTTCTGGCAGTGATTCACATTTAATCAAGCCGGTCCTCAGGAGCAAGAACTTTGTTACAAAAAATATGAAGCAGGATTTGAGTTCTGGTTCTAGTAGTTCCAAATCAGATAACCAGATAGTTGATAATGATTTAGTTTCTGCTACTAGTAAATTGGAGGGTCAGATGCACAGTGGTACTCCCAAGCATGAAAGGGATGGAAATACAAAAAGTTCTCTGATCTCCAGTAATCATAGTGTAGAAATGAACTCAAGTATTGTTGATAGAAAAGGGAAAGCAGATTTCCGGTCAAATTACAGTAACAAGAGCAAATCCATAGTGACAAATGCCGATGAGAATTCAAGATCAAGAGAAAAGGGAGATTTCTCACAAAGCTCAAAAAGTAGCATCGGTGATTACAGTAGCAGTACAAGCACTAGTGAGGAGAGCACTCTAAGTGGGTCTGGTCGAAGTGGCAAGAGGCCTCACATGTCAAAAGACTTGAGATGGGAAGCCATCCATCATGCTCAGAAGCAGCAAGGAAGCTTAGGTTTGAGGCACTTTAAGATGCTCAGGAAGCTTGGTTCTGGGGACATTGGAACCGTTTATCTTGCTGAGCTGACTGGGACAAACTGTGTATTTGCTGTGAAAGTAATGGATAATGAGTCTTTGGCTACCAAGAAGAAAATGATTAGGGCCCAAACAGAAAGAGAGATACTGGAGATACTGGATCATCCATTTCTCCCCACGTTGTATGCTCACTTTGTGTCAGATAAGCTCTCCTGTTTGGTTATGGAGTACTGTCCTGGTGGAGATCTGCATGTGCTGCGACAAAAGCAACCTGGCAGGAGTTTCTGCGAACAAGCAACCAGGTTCTATGTCTCTGAAGTCCTCCTTGCGATGGAGTATTTACACATGCAAGGTGTTGTGTATCGAGACTTGAAACCAGAAAATATCCTGGTCCGAGATGACGGCCACATAATGCTCACAGATTTTGATTTGTCACTTAGATGTGCAGTCAATCCAATGCTACTCAAATCATCGTCTCCAGTTGTGGAGAGCAGAAAAAAGATGGCAAACCCATGTATCGAATCCAGCTGCATTGACCCTTTCTGCCTCCAACCAGCCTGGCAGGGCTCCTGCTTCACTCCTAGGATTTTATCTGCTGGTCCACTCCCACAGCTTGTAGTGGAGCCAACGGATGCCCGATCAAACTCCTTTGTTGGAACCCATGAATACCTTGCTCCAGAGATCATCAAAGGAGAGGGTCATGGAAGTGCTGTTGATTGGTGGACATTTGGAATCTTTCTGTTCGAGCTGCTATATGGTAAGACACCCTTCAAGGGATCAGGAAATGAGGACACATTAGCCAATGTTGTCTCAAAAAGCCTCAAGTTCCCAAACAACCCCATAGTTAGTTTTCATGCACGAGATTTGATCAGAGGCCTCTTAATAAAGGACCCTGAGAATCGTTTAGGATCTGTGAAGGGGGCTGCAGAGATTAAGCAGCATCCTTTCTTTGAAGGCCTTAACTGGGCATTAGTTCGGTGTGCAATACCACCAGAGATGCCCAAATTGTGTGATAGTGTAATTTGTGCACCTCCTGCTCCACAGATGCAGGAGAGCACAGGGTGCAAGGAGTTGGAGGGTACAGTAGAGCATGTAGACTTTGAGCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

818

Amino Acids

89.54

Weight (kDa)

8.18

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 447 - 595 2.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 447 - 596 4.3e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 662 - 765 2.3e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 669 - 749 1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 2439
AciI CCGC 2 cut(s) 355, 551
AclWI GGATC 4 cut(s) 451, 1512, 2128, 2260
AcoI YGGCCR 2 cut(s) 564, 1747
AcsI RAATTY 6 cut(s) 166, 276, 475, 615, 739, 1132
AcuI CTGAAG 3 cut(s) 165, 558, 1680
AdeI CACNNNGTG 2 cut(s) 1540, 2406
AfaI GTAC 9 cut(s) 7, 253, 416, 498, 746, 973, 1204, 1574, 2425
AfeI AGCGCT 1 cut(s) 306
AfiI CCNNNNNNNGG 5 cut(s) 104, 354, 1675, 2117, 2308
AflIII ACRYGT 2 cut(s) 20, 1263
AgsI TTSAA 6 cut(s) 64, 298, 1137, 1717, 2116, 2297
AhlI ACTAGT 2 cut(s) 941, 1211
AjnI CCWGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
AloI GAACNNNNNNTCC 2 cut(s) 1342, 1374
Alw21I GWGCWC 3 cut(s) 1226, 2374, 2402
Alw26I GTCTC 4 cut(s) 1660, 1704, 1824, 2158
Alw44I GTGCAC 1 cut(s) 2370
AlwI GGATC 4 cut(s) 451, 1512, 2128, 2260
AlwNI CAGNNNCTG 1 cut(s) 287
Aor51HI AGCGCT 1 cut(s) 306
AoxI GGCC 9 cut(s) 150, 184, 365, 564, 1256, 1470, 1747, 2218, 2299
ApaI GGGCCC 1 cut(s) 1474
ApaLI GTGCAC 1 cut(s) 2370
ApeKI GCWGC 7 cut(s) 344, 1309, 1600, 1872, 2092, 2267, 2281
ApoI RAATTY 6 cut(s) 166, 276, 475, 615, 739, 1132
AspA2I CCTAGG 1 cut(s) 1925
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 7 cut(s) 185, 829, 1470, 1471, 1735, 1943, 2233
AsuHPI GGTGA 1 cut(s) 1199
AvaII GGWCC 4 cut(s) 829, 1735, 1943, 2233
AvrII CCTAGG 1 cut(s) 1925
AxyI CCTNAGG 1 cut(s) 833
BaeGI GKGCMC 2 cut(s) 1474, 2374
BaeI ACNNNNGTAYC 2 cut(s) 1843, 1876
BanII GRGCYC 2 cut(s) 1474, 1913
BauI CACGAG 1 cut(s) 2202
Bbv12I GWGCWC 3 cut(s) 1226, 2374, 2402
BbvI GCAGC 7 cut(s) 331, 1321, 1587, 1859, 2079, 2254, 2293
BccI CCATC 9 cut(s) 74, 334, 659, 986, 1275, 1298, 1302, 1669, 1840
BceAI ACGGC 1 cut(s) 1762
BciT130I CCWGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
BclI TGATCA 1 cut(s) 2211
BcoDI GTCTC 4 cut(s) 1660, 1704, 1824, 2158
BcuI ACTAGT 2 cut(s) 941, 1211
BfaI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
BfmI CTRYAG 1 cut(s) 2268
BfoI RGCGCY 1 cut(s) 308
BglII AGATCT 2 cut(s) 486, 1588
BisI GCNGC 9 cut(s) 345, 356, 551, 1310, 1601, 1873, 2093, 2268, 2282
BlnI CCTAGG 1 cut(s) 1925
BlpI GCTNAGC 1 cut(s) 1389
BlsI GCNGC 9 cut(s) 346, 357, 552, 1311, 1602, 1874, 2094, 2269, 2283
BmcAI AGTACT 1 cut(s) 1574
Bme1390I CCNGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
Bme18I GGWCC 4 cut(s) 829, 1735, 1943, 2233
BmgT120I GGNCC 7 cut(s) 185, 829, 1470, 1471, 1735, 1943, 2233
BmiI GGNNCC 7 cut(s) 723, 1375, 1472, 1912, 1968, 2002, 2235
BmrFI CCNGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
BmrI ACTGGG 2 cut(s) 1407, 2317
BmuI ACTGGG 2 cut(s) 1407, 2317
BpmI CTGGAG 4 cut(s) 1003, 1514, 1806, 2004
Bpu10I CCTNAGC 2 cut(s) 1321, 1346
Bpu1102I GCTNAGC 1 cut(s) 1389
BpuEI CTTGAG 4 cut(s) 235, 1030, 1297, 2150
BsaJI CCNNGG 2 cut(s) 188, 1925
BsaWI WCCGGW 1 cut(s) 1080
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 354, 1675, 2117, 2308
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2312
Bse21I CCTNAGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 95, 591
BseBI CCWGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
BseDI CCNNGG 2 cut(s) 188, 1925
BseGI GGATG 8 cut(s) 76, 133, 559, 997, 1290, 1507, 1981, 2284
BseLI CCNNNNNNNGG 5 cut(s) 104, 354, 1675, 2117, 2308
BseMI GCAATG 2 cut(s) 95, 591
BseMII CTCAG 9 cut(s) 117, 322, 341, 533, 847, 1316, 1360, 1380, 2229
BseNI ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2312
BseRI GAGGAG 1 cut(s) 1232
BseSI GKGCMC 2 cut(s) 1474, 2374
BseXI GCAGC 7 cut(s) 331, 1321, 1587, 1859, 2079, 2254, 2293
BsgI GTGCAG 1 cut(s) 1809
BshFI GGCC 9 cut(s) 152, 186, 367, 566, 1258, 1472, 1749, 2220, 2301
BsiHKAI GWGCWC 3 cut(s) 1226, 2374, 2402
BsiSI CCGG 3 cut(s) 563, 827, 1081
BslFI GGGAC 2 cut(s) 1379, 1414
BslI CCNNNNNNNGG 5 cut(s) 104, 354, 1675, 2117, 2308
BsmAI GTCTC 4 cut(s) 1660, 1704, 1824, 2158
BsmBI CGTCTC 1 cut(s) 1824
BsmFI GGGAC 2 cut(s) 1379, 1414
BsnI GGCC 9 cut(s) 152, 186, 367, 566, 1258, 1472, 1749, 2220, 2301
Bsp120I GGGCCC 1 cut(s) 1470
Bsp1286I GDGCHC 5 cut(s) 1226, 1474, 1913, 2374, 2402
Bsp1407I TGTACA 1 cut(s) 414
Bsp1720I GCTNAGC 1 cut(s) 1389
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 2 cut(s) 355, 551
BspANI GGCC 9 cut(s) 152, 186, 367, 566, 1258, 1472, 1749, 2220, 2301
BspCNI CTCAG 9 cut(s) 116, 321, 340, 532, 846, 1315, 1359, 1381, 2230
BspLI GGNNCC 7 cut(s) 723, 1375, 1472, 1912, 1968, 2002, 2235
BspMAI CTGCAG 1 cut(s) 2272
BspPI GGATC 4 cut(s) 451, 1512, 2128, 2260
BsrDI GCAATG 2 cut(s) 95, 591
BsrFI RCCGGY 1 cut(s) 826
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2312
BssAI RCCGGY 1 cut(s) 826
BssECI CCNNGG 2 cut(s) 188, 1925
BssSI CACGAG 1 cut(s) 2202
BssT1I CCWWGG 2 cut(s) 188, 1925
Bst2BI CACGAG 1 cut(s) 2202
Bst2UI CCWGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
Bst4CI ACNGT 7 cut(s) 968, 1094, 1196, 1378, 1409, 1577, 2428
Bst6I CTCTTC 1 cut(s) 215
BstAPI GCANNNNNTGC 4 cut(s) 94, 1600, 2378, 2406
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 86, 303
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 8 cut(s) 76, 133, 559, 997, 1290, 1507, 1981, 2284
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstMAI GTCTC 4 cut(s) 1660, 1704, 1824, 2158
BstMWI GCNNNNNNNGC 9 cut(s) 94, 364, 547, 1206, 1351, 1600, 1609, 2378, 2406
BstNI CCWGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
BstNSI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2438
BstSCI CCNGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
BstSFI CTRYAG 1 cut(s) 2268
BstSLI GKGCMC 2 cut(s) 1474, 2374
BstV1I GCAGC 7 cut(s) 331, 1321, 1587, 1859, 2079, 2254, 2293
BstX2I RGATCY 4 cut(s) 443, 486, 1588, 2252
BstYI RGATCY 4 cut(s) 443, 486, 1588, 2252
Bsu36I CCTNAGG 1 cut(s) 833
BsuRI GGCC 9 cut(s) 152, 186, 367, 566, 1258, 1472, 1749, 2220, 2301
BtgI CCRYGG 1 cut(s) 188
BtgZI GCGATG 1 cut(s) 1688
BtsCI GGATG 8 cut(s) 76, 133, 559, 997, 1290, 1507, 1981, 2284
BtsI GCAGTG 2 cut(s) 429, 811
BtsIMutI CAGTG 3 cut(s) 429, 811, 973
Cac8I GCNNGC 2 cut(s) 86, 303
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 826
Cfr13I GGNCC 7 cut(s) 185, 829, 1470, 1471, 1735, 1943, 2233
CsiI ACCWGGT 1 cut(s) 1643
Csp6I GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2424
CviQI GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2424
DraIII CACNNNGTG 2 cut(s) 1540, 2406
EaeI YGGCCR 2 cut(s) 564, 1747
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 188, 1925
Eco147I AGGCCT 3 cut(s) 1258, 2220, 2301
Eco24I GRGCYC 2 cut(s) 1474, 1913
Eco47I GGWCC 4 cut(s) 829, 1735, 1943, 2233
Eco47III AGCGCT 1 cut(s) 306
Eco57I CTGAAG 3 cut(s) 165, 558, 1680
Eco81I CCTNAGG 1 cut(s) 833
EcoO109I RGGNCCY 2 cut(s) 1470, 2233
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
EcoT14I CCWWGG 2 cut(s) 188, 1925
EcoT22I ATGCAT 2 cut(s) 323, 508
EcoT38I GRGCYC 2 cut(s) 1474, 1913
ErhI CCWWGG 2 cut(s) 188, 1925
Esp3I CGTCTC 1 cut(s) 1824
FalI AAGNNNNNCTT 6 cut(s) 781, 813, 1653, 1685, 2096, 2128
FaqI GGGAC 2 cut(s) 1379, 1414
FbaI TGATCA 1 cut(s) 2211
FblI GTMKAC 1 cut(s) 2439
Fnu4HI GCNGC 9 cut(s) 345, 356, 551, 1310, 1601, 1873, 2093, 2268, 2282
FokI GGATG 8 cut(s) 83, 140, 546, 1004, 1277, 1494, 1988, 2271
FriOI GRGCYC 2 cut(s) 1474, 1913
Fsp4HI GCNGC 9 cut(s) 345, 356, 551, 1310, 1601, 1873, 2093, 2268, 2282
FspBI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
GlaI GCGC 1 cut(s) 306
GluI GCNGC 9 cut(s) 345, 356, 551, 1310, 1601, 1873, 2093, 2268, 2282
GsuI CTGGAG 4 cut(s) 1003, 1514, 1806, 2004
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 9 cut(s) 152, 186, 367, 566, 1258, 1472, 1749, 2220, 2301
HapII CCGG 3 cut(s) 563, 827, 1081
HhaI GCGC 1 cut(s) 307
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 3 cut(s) 140, 1318, 1352
HinfI GANTC 7 cut(s) 53, 534, 809, 1439, 1865, 2076, 2242
HpaII CCGG 3 cut(s) 563, 827, 1081
HphI GGTGA 1 cut(s) 1199
Hpy166II GTNNAC 4 cut(s) 1946, 2067, 2372, 2440
Hpy188III TCNNGA 8 cut(s) 105, 835, 1137, 1143, 1348, 1709, 2021, 2124
Hpy8I GTNNAC 4 cut(s) 1946, 2067, 2372, 2440
HpyAV CCTTC 4 cut(s) 458, 2122, 2254, 2291
HpyCH4III ACNGT 7 cut(s) 968, 1094, 1196, 1378, 1409, 1577, 2428
HpyCH4IV ACGT 1 cut(s) 1523
HpyF10VI GCNNNNNNNGC 9 cut(s) 94, 364, 547, 1206, 1351, 1600, 1609, 2378, 2406
HpySE526I ACGT 1 cut(s) 1523
HspAI GCGC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 2211
LmnI GCTCC 5 cut(s) 837, 1916, 1966, 2023, 2386
Lsp1109I GCAGC 7 cut(s) 331, 1321, 1587, 1859, 2079, 2254, 2293
MabI ACCWGGT 1 cut(s) 1643
MaeI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
MaeII ACGT 1 cut(s) 1523
MaeIII GTNAC 4 cut(s) 850, 1094, 1114, 1776
MboII GAAGA 9 cut(s) 56, 232, 612, 615, 674, 689, 759, 789, 1468
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 4 cut(s) 443, 486, 1588, 2252
MhlI GDGCHC 5 cut(s) 1226, 1474, 1913, 2374, 2402
MlyI GAGTC 1 cut(s) 1448
MmeI TCCRAC 3 cut(s) 1918, 1978, 2397
Mph1103I ATGCAT 2 cut(s) 323, 508
MseI TTAA 9 cut(s) 135, 405, 654, 788, 818, 1338, 2225, 2277, 2304
MslI CAYNNNNRTG 3 cut(s) 713, 1783, 2049
MspA1I CMGCKG 2 cut(s) 578, 1872
MspI CCGG 3 cut(s) 563, 827, 1081
MspR9I CCNGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
MwoI GCNNNNNNNGC 9 cut(s) 94, 364, 547, 1206, 1351, 1600, 1609, 2378, 2406
NcoI CCATGG 1 cut(s) 188
NlaIV GGNNCC 7 cut(s) 723, 1375, 1472, 1912, 1968, 2002, 2235
NmuCI GTSAC 2 cut(s) 1114, 1776
NsiI ATGCAT 2 cut(s) 323, 508
NspI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2438
PceI AGGCCT 3 cut(s) 1258, 2220, 2301
PciI ACATGT 2 cut(s) 20, 1263
PfeI GAWTC 6 cut(s) 53, 534, 809, 1865, 2076, 2242
PkrI GCNGC 9 cut(s) 346, 357, 552, 1311, 1602, 1874, 2094, 2269, 2283
PleI GAGTC 1 cut(s) 1447
PpsI GAGTC 1 cut(s) 1447
PpuMI RGGWCCY 1 cut(s) 2233
PscI ACATGT 2 cut(s) 20, 1263
Psp5II RGGWCCY 1 cut(s) 2233
Psp6I CCWGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
PspGI CCWGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
PspN4I GGNNCC 7 cut(s) 723, 1375, 1472, 1912, 1968, 2002, 2235
PspOMI GGGCCC 1 cut(s) 1470
PspPI GGNCC 7 cut(s) 185, 829, 1470, 1471, 1735, 1943, 2233
PspPPI RGGWCCY 1 cut(s) 2233
PstI CTGCAG 1 cut(s) 2272
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 4 cut(s) 443, 486, 1588, 2252
PvuII CAGCTG 2 cut(s) 578, 1872
RsaI GTAC 9 cut(s) 7, 253, 416, 498, 746, 973, 1204, 1574, 2425
RsaNI GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2424
RseI CAYNNNNRTG 3 cut(s) 713, 1783, 2049
SaqAI TTAA 9 cut(s) 135, 405, 654, 788, 818, 1338, 2225, 2277, 2304
SatI GCNGC 9 cut(s) 345, 356, 551, 1310, 1601, 1873, 2093, 2268, 2282
Sau96I GGNCC 7 cut(s) 185, 829, 1470, 1471, 1735, 1943, 2233
ScaI AGTACT 1 cut(s) 1574
SchI GAGTC 1 cut(s) 1448
ScrFI CCNGG 7 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903
SduI GDGCHC 5 cut(s) 1226, 1474, 1913, 2374, 2402
SexAI ACCWGGT 1 cut(s) 1643
SfcI CTRYAG 1 cut(s) 2268
SinI GGWCC 4 cut(s) 829, 1735, 1943, 2233
SmiMI CAYNNNNRTG 3 cut(s) 713, 1783, 2049
SmlI CTYRAG 4 cut(s) 214, 1045, 1276, 2165
SmoI CTYRAG 4 cut(s) 214, 1045, 1276, 2165
SpeI ACTAGT 2 cut(s) 941, 1211
SseBI AGGCCT 3 cut(s) 1258, 2220, 2301
SsiI CCGC 2 cut(s) 355, 551
SspMI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
StuI AGGCCT 3 cut(s) 1258, 2220, 2301
StyD4I CCNGG 7 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901
StyI CCWWGG 2 cut(s) 188, 1925
TaaI ACNGT 7 cut(s) 968, 1094, 1196, 1378, 1409, 1577, 2428
TaiI ACGT 1 cut(s) 1526
TaqI TCGA 8 cut(s) 33, 119, 362, 432, 1243, 1708, 1863, 2088
TatI WGTACW 5 cut(s) 5, 251, 414, 1202, 1572
TauI GCSGC 2 cut(s) 358, 553
TfiI GAWTC 6 cut(s) 53, 534, 809, 1865, 2076, 2242
Tru1I TTAA 9 cut(s) 135, 405, 654, 788, 818, 1338, 2225, 2277, 2304
Tru9I TTAA 9 cut(s) 135, 405, 654, 788, 818, 1338, 2225, 2277, 2304
TscAI CASTG 3 cut(s) 429, 811, 973
TseFI GTSAC 2 cut(s) 1114, 1776
TseI GCWGC 7 cut(s) 344, 1309, 1600, 1872, 2092, 2267, 2281
Tsp45I GTSAC 2 cut(s) 1114, 1776
TspGWI ACGGA 1 cut(s) 1988
TspRI CASTG 3 cut(s) 429, 811, 973
VneI GTGCAC 1 cut(s) 2370
VpaK11BI GGWCC 4 cut(s) 829, 1735, 1943, 2233
XapI RAATTY 6 cut(s) 166, 276, 475, 615, 739, 1132
XceI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2438
XmaJI CCTAGG 1 cut(s) 1925
XmiI GTMKAC 1 cut(s) 2439
XspI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
ZrmI AGTACT 1 cut(s) 1574
Zsp2I ATGCAT 2 cut(s) 323, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.