RLG00000034218

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007496
Physical Location & Seq
Forward (+)
38658930 .. 38662435
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034218

Sequence Viewer

Length: 2502 bp
ATGAGTACATCAGGCATTACATGTGAGATTGTCGAAGCAGGAGAAGAAGTAGATTCTCAATTGAAAATGGATAAGAATGATGGCAAGCAATGTGTGCCAAACTCAGGAAATAGGTATTCAATAGAGGATGATATTAACAAGCTTTTTCAGGCCATTGATGTTAGAAATTCAGGCAGGATTTCAAGCCCATGGCATGAAAGTAGCAAAGATGCCTTGAGGAAGAGTGCTATGAAAAAGCCAATGAGAGCTAGTACATCAAAAGTATCAGGTATAGGAATTTCAGAGCCTGTAAGTTTGAAGCAAGCGCTCAGAGGATTATGCATCTCTCAGGCATCAGAGATGGCTGCCATGAAGCGGCTATCGAGGCCAGGTCGTTCATCTGGGGTTTCAGAAGCAGGAACTATTAAAAGGTTGTACAGGGCAGTGGTAGTCGAGGAAAATGGGTCTGACCTCCCAATGAATGAAGGTAAAGGGAATTTTGTAGAGATCTCCTTAGTACCAGATGCATCAAATAGCTCTCAGCAGATGCCTGGATTCATGCAATCAGAGCCACAGTTTCATCCAGCCACAACTTCAGCTGATGCAATGATGACCAAATTAGCACCACAGAAACAAATTTCTTCGTCACCAACAGCAGTTCAGAGTAAGATGTTAAAGGGAGAAGATGGAAAACTGGAAGATGCAGATACTTCATCAAGCAAAAAAGTCATGCAGGTGGAGATTCCAATCACTACTCTCAATTTGTACAAGGATCAGCACTCTGCTTCTTCTCTATCTGGCAGTAAGTTAAGGAAATCTTCTGGCAGTGATTCACATTTAATCAAGCCGGTCCTGAGGAGCAAGAACATTGTTACAAAAAATATGAAGCCGGATTTGAGTTCTGGTTCTAGTAGTTCCAAATCAGATAACCAGATAATTGATAATGATTTAGTTTCTACTACTAGTAAATTGGAGGGTCAGACGCACAGTGGTACTCCAAAGCATGAAAGGGATGGAAATACAAAAAGTTCTCTGATCTCCAGTAGTAATAGTGTAGAAATGAACTCAGGTATTGTTGATAGAAAAGGGAAAGCAGATTTCGGGTCAAATTACAGTAACAGGAGCAAATCCATAGTGACAAATGCTGATGAGAGTTCAAGATCAAGAGAAAAGGGAGATTTCTCACAAAGCTCGAAAAGTAGCATCGGTGATTACAGTAGCAGTACAAGCACTAGTGAGGAGAGCACTCTAAGTGGATCTGGTCGAAGTGGCAAGAGGCCTCACATGTCAAAAGACTTGAGATGGGAAGCCATCCATCATGCTCAGAAGCAGCAAGGAAGCTTAGGTTTGAGGCACTTTAAGATGCTCAGGAAGCTTGGTTCTGGGGACATTGGAACCGTTTATCTTGCTGAGCTGACTGGGACAAACTGTGTATTTGCTGTGAAAGTAATGGATAATGAGTCTTTGGCTACCAAGAAGAAAATGATTAGGGCCCAAACAGAAAGAGAGATACTGGAGATACTGGATCATCCTTTTCTCCCTACACTGTATGCTCACTTTGTGTCAGATAAGCTCTCCTGTTTGGTTATGGAGTACTGTCCTGGTGGAGATCTACATGTGCTGCGACAAAAGCAACCTGGCAGGAGTTTCTGCGAACAAGCAGCCAGGTTCTATGTCTCTGAAGTCCTCCTTGCGCTGGAGTATTTACACATGCAAGGTGTTGTGTATCGAGACTTGAAACCAGAAAATATCCTGGTCCGAGATGACGGCCACATAATGCTCACAGATTTTGATTTGTCACTTAGGTGTGCAGTCAATCCAATGCTACTCAAATCATCGTCTCCAGTTGTGGAGAGCAGAAAAAAGATGGCAAACCCATGTATCGAATCCAGCTGCATTGACCCTTTCTGCCTCCAACCAGCCTGGCAGGGCTCCTGCTTCACTCCTAGGATTTTATCTGCTGGTGCAAAATCTCGGAAAATAAAATCTGAACTTGCTGCCCAGGTCAGTCCACTTCCACAGCTTGTAGTGGAGCCAACGGATGCCCGATCAAACTCCTTTGTTGGAACCCATGAATACCTTGCTCCAGAGATCATCAAAGGAGAGGGTCATGGGAGTGCCGTTGATTGGTGGACATTTGGAATCTTTCTGTTCGAGCTGCTATATGGTAAGACACCCTTCAAGGGATCAGGAAATGAGGACACATTAGCCAATGTTGTCTCGAAAAGTCTCAAGTTCCCAAACAACCCTATAGTTAGTTTTCATGCACGAGATTTGATCAGAGGCCTCTTAATAAAGGACCCTGAGAATCGTTTAGGATCTGTGAAGGGGGCTGCAGAGATTAAGCAGCATCCTTTCTTTGAAGGCCTTAACTGGGCATTAGTTCGGTGTGCAATACCACCAGAGATGCCAAAATTGTGTGATAGTGTAATTTGTGCACCTCCTGCTCCACAGATGCAGGAGAGCACAGGGTGCAAGGAGTTGGAGGGTACAGTAGAGCATGTAGACTTTGAGCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

834

Amino Acids

91.02

Weight (kDa)

8.4

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 447 - 595 7.8e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 447 - 596 3.9e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 677 - 780 2.3e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 684 - 764 1.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 2484
AciI CCGC 1 cut(s) 355
AclWI GGATC 5 cut(s) 759, 1243, 1512, 2173, 2305
AcoI YGGCCR 1 cut(s) 1747
AcsI RAATTY 4 cut(s) 166, 276, 475, 615
AcuI CTGAAG 2 cut(s) 558, 1680
AdeI CACNNNGTG 2 cut(s) 1540, 2451
AfaI GTAC 9 cut(s) 7, 253, 416, 498, 746, 973, 1204, 1574, 2470
AfeI AGCGCT 1 cut(s) 306
AfiI CCNNNNNNNGG 6 cut(s) 104, 354, 1675, 2106, 2162, 2353
AflIII ACRYGT 3 cut(s) 20, 1263, 1594
AgsI TTSAA 8 cut(s) 64, 120, 183, 298, 1137, 1717, 2161, 2342
AhlI ACTAGT 2 cut(s) 941, 1211
AjnI CCWGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
AleI CACNNNNGTG 1 cut(s) 1783
AloI GAACNNNNNNTCC 2 cut(s) 1342, 1374
Alw21I GWGCWC 3 cut(s) 1226, 2419, 2447
Alw26I GTCTC 5 cut(s) 1660, 1704, 1824, 2203, 2213
Alw44I GTGCAC 1 cut(s) 2415
AlwI GGATC 5 cut(s) 759, 1243, 1512, 2173, 2305
AlwNI CAGNNNCTG 1 cut(s) 287
Aor51HI AGCGCT 1 cut(s) 306
AoxI GGCC 7 cut(s) 150, 365, 1256, 1470, 1747, 2263, 2344
ApaI GGGCCC 1 cut(s) 1474
ApaLI GTGCAC 1 cut(s) 2415
ApeKI GCWGC 9 cut(s) 344, 1309, 1600, 1640, 1872, 1976, 2137, 2312, 2326
ApoI RAATTY 4 cut(s) 166, 276, 475, 615
AspA2I CCTAGG 1 cut(s) 1925
AspLEI GCGC 2 cut(s) 307, 1675
AspS9I GGNCC 5 cut(s) 829, 1470, 1471, 1735, 2278
AsuHPI GGTGA 2 cut(s) 618, 1199
AvaII GGWCC 3 cut(s) 829, 1735, 2278
AvrII CCTAGG 1 cut(s) 1925
AxyI CCTNAGG 1 cut(s) 833
BaeGI GKGCMC 2 cut(s) 1474, 2419
BaeI ACNNNNGTAYC 2 cut(s) 1843, 1876
BanII GRGCYC 2 cut(s) 1474, 1913
BauI CACGAG 1 cut(s) 2247
Bbv12I GWGCWC 3 cut(s) 1226, 2419, 2447
BbvI GCAGC 9 cut(s) 331, 1321, 1587, 1652, 1859, 1963, 2124, 2299, 2338
BccI CCATC 8 cut(s) 74, 334, 659, 986, 1275, 1298, 1302, 1840
BceAI ACGGC 2 cut(s) 1762, 2084
BciT130I CCWGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
BclI TGATCA 1 cut(s) 2256
BcoDI GTCTC 5 cut(s) 1660, 1704, 1824, 2203, 2213
BcuI ACTAGT 2 cut(s) 941, 1211
BfaI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
BfmI CTRYAG 2 cut(s) 2229, 2313
BfoI RGCGCY 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 703
BglII AGATCT 2 cut(s) 486, 1588
BlnI CCTAGG 1 cut(s) 1925
BlpI GCTNAGC 1 cut(s) 1389
BmcAI AGTACT 1 cut(s) 1574
Bme1390I CCNGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
Bme18I GGWCC 3 cut(s) 829, 1735, 2278
BmgT120I GGNCC 5 cut(s) 829, 1470, 1471, 1735, 2278
BmiI GGNNCC 6 cut(s) 1375, 1472, 1912, 2013, 2047, 2280
BmrFI CCNGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
BmrI ACTGGG 2 cut(s) 1407, 2362
BmuI ACTGGG 2 cut(s) 1407, 2362
BpmI CTGGAG 5 cut(s) 1003, 1514, 1697, 1806, 2049
Bpu10I CCTNAGC 2 cut(s) 1321, 1346
Bpu1102I GCTNAGC 1 cut(s) 1389
BpuEI CTTGAG 3 cut(s) 235, 1297, 2195
BsaBI GATNNNNATC 1 cut(s) 725
BsaJI CCNNGG 3 cut(s) 188, 1925, 1980
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 354, 1675, 2106, 2162, 2353
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2357
Bse21I CCTNAGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 95, 591
Bse8I GATNNNNATC 1 cut(s) 725
BseBI CCWGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
BseDI CCNNGG 3 cut(s) 188, 1925, 1980
BseGI GGATG 7 cut(s) 133, 559, 997, 1290, 1507, 2026, 2329
BseJI GATNNNNATC 1 cut(s) 725
BseLI CCNNNNNNNGG 6 cut(s) 104, 354, 1675, 2106, 2162, 2353
BseMI GCAATG 2 cut(s) 95, 591
BseNI ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2357
BseRI GAGGAG 2 cut(s) 850, 1232
BseSI GKGCMC 2 cut(s) 1474, 2419
BseXI GCAGC 9 cut(s) 331, 1321, 1587, 1652, 1859, 1963, 2124, 2299, 2338
BsgI GTGCAG 1 cut(s) 1809
BshFI GGCC 7 cut(s) 152, 367, 1258, 1472, 1749, 2265, 2346
BsiHKAI GWGCWC 3 cut(s) 1226, 2419, 2447
BsiSI CCGG 2 cut(s) 827, 869
BslFI GGGAC 2 cut(s) 1379, 1414
BslI CCNNNNNNNGG 6 cut(s) 104, 354, 1675, 2106, 2162, 2353
BsmAI GTCTC 5 cut(s) 1660, 1704, 1824, 2203, 2213
BsmBI CGTCTC 1 cut(s) 1824
BsmFI GGGAC 2 cut(s) 1379, 1414
BsnI GGCC 7 cut(s) 152, 367, 1258, 1472, 1749, 2265, 2346
Bsp120I GGGCCC 1 cut(s) 1470
Bsp1286I GDGCHC 5 cut(s) 1226, 1474, 1913, 2419, 2447
Bsp1407I TGTACA 2 cut(s) 414, 744
Bsp1720I GCTNAGC 1 cut(s) 1389
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 1 cut(s) 355
BspANI GGCC 7 cut(s) 152, 367, 1258, 1472, 1749, 2265, 2346
BspLI GGNNCC 6 cut(s) 1375, 1472, 1912, 2013, 2047, 2280
BspMAI CTGCAG 1 cut(s) 2317
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 5 cut(s) 759, 1243, 1512, 2173, 2305
BsrDI GCAATG 2 cut(s) 95, 591
BsrFI RCCGGY 1 cut(s) 826
BsrGI TGTACA 2 cut(s) 414, 744
BsrI ACTGG 7 cut(s) 678, 1020, 1402, 1497, 1506, 1823, 2357
BssAI RCCGGY 1 cut(s) 826
BssECI CCNNGG 3 cut(s) 188, 1925, 1980
BssSI CACGAG 1 cut(s) 2247
BssT1I CCWWGG 2 cut(s) 188, 1925
Bst2BI CACGAG 1 cut(s) 2247
Bst2UI CCWGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
Bst4CI ACNGT 9 cut(s) 555, 968, 1094, 1196, 1378, 1409, 1527, 1577, 2473
Bst6I CTCTTC 1 cut(s) 215
BstAPI GCANNNNNTGC 3 cut(s) 94, 2423, 2451
BstAUI TGTACA 2 cut(s) 414, 744
BstC8I GCNNGC 2 cut(s) 86, 303
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 7 cut(s) 133, 559, 997, 1290, 1507, 2026, 2329
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 2 cut(s) 307, 1675
BstMAI GTCTC 5 cut(s) 1660, 1704, 1824, 2203, 2213
BstMWI GCNNNNNNNGC 8 cut(s) 94, 364, 547, 1206, 1351, 1609, 2423, 2451
BstNI CCWGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
BstNSI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2483
BstSCI CCNGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
BstSFI CTRYAG 2 cut(s) 2229, 2313
BstSLI GKGCMC 2 cut(s) 1474, 2419
BstV1I GCAGC 9 cut(s) 331, 1321, 1587, 1652, 1859, 1963, 2124, 2299, 2338
BstX2I RGATCY 4 cut(s) 486, 1235, 1588, 2297
BstYI RGATCY 4 cut(s) 486, 1235, 1588, 2297
Bsu36I CCTNAGG 1 cut(s) 833
BsuRI GGCC 7 cut(s) 152, 367, 1258, 1472, 1749, 2265, 2346
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 7 cut(s) 133, 559, 997, 1290, 1507, 2026, 2329
BtsI GCAGTG 2 cut(s) 429, 811
BtsIMutI CAGTG 4 cut(s) 429, 811, 973, 1523
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 86, 303
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 2 cut(s) 307, 1675
Cfr10I RCCGGY 1 cut(s) 826
Cfr13I GGNCC 5 cut(s) 829, 1470, 1471, 1735, 2278
CseI GACGC 1 cut(s) 970
Csp6I GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2469
CviQI GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2469
DraIII CACNNNGTG 2 cut(s) 1540, 2451
EaeI YGGCCR 1 cut(s) 1747
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 188, 1925
Eco147I AGGCCT 3 cut(s) 1258, 2265, 2346
Eco24I GRGCYC 2 cut(s) 1474, 1913
Eco47I GGWCC 3 cut(s) 829, 1735, 2278
Eco47III AGCGCT 1 cut(s) 306
Eco57I CTGAAG 2 cut(s) 558, 1680
Eco81I CCTNAGG 1 cut(s) 833
EcoO109I RGGNCCY 2 cut(s) 1470, 2278
EcoRII CCWGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
EcoT14I CCWWGG 2 cut(s) 188, 1925
EcoT22I ATGCAT 2 cut(s) 323, 508
EcoT38I GRGCYC 2 cut(s) 1474, 1913
ErhI CCWWGG 2 cut(s) 188, 1925
Esp3I CGTCTC 1 cut(s) 1824
FalI AAGNNNNNCTT 6 cut(s) 781, 813, 1653, 1685, 2141, 2173
FaqI GGGAC 2 cut(s) 1379, 1414
FbaI TGATCA 1 cut(s) 2256
FblI GTMKAC 1 cut(s) 2484
FokI GGATG 7 cut(s) 140, 546, 1004, 1277, 1494, 2033, 2316
FriOI GRGCYC 2 cut(s) 1474, 1913
FspBI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
GlaI GCGC 2 cut(s) 306, 1674
GsuI CTGGAG 5 cut(s) 1003, 1514, 1697, 1806, 2049
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 7 cut(s) 152, 367, 1258, 1472, 1749, 2265, 2346
HapII CCGG 2 cut(s) 827, 869
HgaI GACGC 1 cut(s) 970
HhaI GCGC 2 cut(s) 307, 1675
Hin6I GCGC 2 cut(s) 305, 1673
HinP1I GCGC 2 cut(s) 305, 1673
HindIII AAGCTT 3 cut(s) 140, 1318, 1352
HinfI GANTC 8 cut(s) 53, 534, 721, 809, 1439, 1865, 2121, 2287
HpaII CCGG 2 cut(s) 827, 869
HphI GGTGA 2 cut(s) 618, 1199
Hpy166II GTNNAC 4 cut(s) 1991, 2112, 2417, 2485
Hpy188III TCNNGA 9 cut(s) 105, 832, 1137, 1143, 1348, 1709, 2066, 2169, 2200
Hpy8I GTNNAC 4 cut(s) 1991, 2112, 2417, 2485
HpyAV CCTTC 4 cut(s) 458, 2167, 2299, 2336
HpyCH4III ACNGT 9 cut(s) 555, 968, 1094, 1196, 1378, 1409, 1527, 1577, 2473
HpyF10VI GCNNNNNNNGC 8 cut(s) 94, 364, 547, 1206, 1351, 1609, 2423, 2451
HspAI GCGC 2 cut(s) 305, 1673
Ksp22I TGATCA 1 cut(s) 2256
LmnI GCTCC 6 cut(s) 837, 1101, 1916, 2011, 2068, 2431
Lsp1109I GCAGC 9 cut(s) 331, 1321, 1587, 1652, 1859, 1963, 2124, 2299, 2338
MaeI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
MaeIII GTNAC 5 cut(s) 624, 850, 1094, 1114, 1776
MboII GAAGA 8 cut(s) 56, 232, 612, 674, 689, 759, 789, 1468
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 4 cut(s) 486, 1235, 1588, 2297
MhlI GDGCHC 5 cut(s) 1226, 1474, 1913, 2419, 2447
MlyI GAGTC 1 cut(s) 1448
MmeI TCCRAC 3 cut(s) 1918, 2023, 2442
Mph1103I ATGCAT 2 cut(s) 323, 508
MseI TTAA 9 cut(s) 135, 405, 653, 788, 818, 1338, 2270, 2322, 2349
MslI CAYNNNNRTG 3 cut(s) 713, 1783, 2094
MspA1I CMGCKG 2 cut(s) 578, 1872
MspI CCGG 2 cut(s) 827, 869
MspR9I CCNGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
MwoI GCNNNNNNNGC 8 cut(s) 94, 364, 547, 1206, 1351, 1609, 2423, 2451
NcoI CCATGG 1 cut(s) 188
NlaIV GGNNCC 6 cut(s) 1375, 1472, 1912, 2013, 2047, 2280
NmuCI GTSAC 3 cut(s) 624, 1114, 1776
NsiI ATGCAT 2 cut(s) 323, 508
NspI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2483
OliI CACNNNNGTG 1 cut(s) 1783
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 3 cut(s) 1258, 2265, 2346
PciI ACATGT 3 cut(s) 20, 1263, 1594
PfeI GAWTC 7 cut(s) 53, 534, 721, 809, 1865, 2121, 2287
PleI GAGTC 1 cut(s) 1447
PpsI GAGTC 1 cut(s) 1447
PpuMI RGGWCCY 1 cut(s) 2278
PscI ACATGT 3 cut(s) 20, 1263, 1594
Psp5II RGGWCCY 1 cut(s) 2278
Psp6I CCWGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
PspGI CCWGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
PspN4I GGNNCC 6 cut(s) 1375, 1472, 1912, 2013, 2047, 2280
PspOMI GGGCCC 1 cut(s) 1470
PspPI GGNCC 5 cut(s) 829, 1470, 1471, 1735, 2278
PspPPI RGGWCCY 1 cut(s) 2278
PstI CTGCAG 1 cut(s) 2317
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 4 cut(s) 486, 1235, 1588, 2297
PvuII CAGCTG 2 cut(s) 578, 1872
RsaI GTAC 9 cut(s) 7, 253, 416, 498, 746, 973, 1204, 1574, 2470
RsaNI GTAC 9 cut(s) 6, 252, 415, 497, 745, 972, 1203, 1573, 2469
RseI CAYNNNNRTG 3 cut(s) 713, 1783, 2094
SaqAI TTAA 9 cut(s) 135, 405, 653, 788, 818, 1338, 2270, 2322, 2349
Sau96I GGNCC 5 cut(s) 829, 1470, 1471, 1735, 2278
ScaI AGTACT 1 cut(s) 1574
SchI GAGTC 1 cut(s) 1448
ScrFI CCNGG 8 cut(s) 369, 531, 1581, 1617, 1645, 1733, 1903, 1982
SduI GDGCHC 5 cut(s) 1226, 1474, 1913, 2419, 2447
SfcI CTRYAG 2 cut(s) 2229, 2313
SinI GGWCC 3 cut(s) 829, 1735, 2278
SmiMI CAYNNNNRTG 3 cut(s) 713, 1783, 2094
SmlI CTYRAG 3 cut(s) 214, 1276, 2210
SmoI CTYRAG 3 cut(s) 214, 1276, 2210
SpeI ACTAGT 2 cut(s) 941, 1211
SseBI AGGCCT 3 cut(s) 1258, 2265, 2346
SsiI CCGC 1 cut(s) 355
SspMI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
StuI AGGCCT 3 cut(s) 1258, 2265, 2346
StyD4I CCNGG 8 cut(s) 367, 529, 1579, 1615, 1643, 1731, 1901, 1980
StyI CCWWGG 2 cut(s) 188, 1925
TaaI ACNGT 9 cut(s) 555, 968, 1094, 1196, 1378, 1409, 1527, 1577, 2473
TaqI TCGA 9 cut(s) 33, 362, 432, 1172, 1243, 1708, 1863, 2133, 2201
TatI WGTACW 6 cut(s) 5, 251, 414, 744, 1202, 1572
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 7 cut(s) 53, 534, 721, 809, 1865, 2121, 2287
Tru1I TTAA 9 cut(s) 135, 405, 653, 788, 818, 1338, 2270, 2322, 2349
Tru9I TTAA 9 cut(s) 135, 405, 653, 788, 818, 1338, 2270, 2322, 2349
TscAI CASTG 4 cut(s) 429, 811, 973, 1530
TseFI GTSAC 3 cut(s) 624, 1114, 1776
TseI GCWGC 9 cut(s) 344, 1309, 1600, 1640, 1872, 1976, 2137, 2312, 2326
Tsp45I GTSAC 3 cut(s) 624, 1114, 1776
TspGWI ACGGA 1 cut(s) 2033
TspRI CASTG 4 cut(s) 429, 811, 973, 1530
VneI GTGCAC 1 cut(s) 2415
VpaK11BI GGWCC 3 cut(s) 829, 1735, 2278
XapI RAATTY 4 cut(s) 166, 276, 475, 615
XceI RCATGY 5 cut(s) 24, 1267, 1598, 1693, 2483
XmaJI CCTAGG 1 cut(s) 1925
XmiI GTMKAC 1 cut(s) 2484
XspI CTAG 5 cut(s) 249, 888, 942, 1212, 1926
ZrmI AGTACT 1 cut(s) 1574
Zsp2I ATGCAT 2 cut(s) 323, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.