pycom03g12510

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007957
Physical Location & Seq
Reverse (-)
13089454 .. 13108351
18898 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5244 bp
ATGGCTTTTTTTAGGAACTATTCTAACAACACAGTTTCACGAAGTGTCCTGGAAGAAAAGACCCTAGGACAAAGTGTTGAAAGAATTCATAGCTCCAAAGGGAATGAAGATATGGATGTAAATTCTTATGAAAAGGAATGTGATATCAACATGGATGTTCAGTATCAAAGTGAAGGCGAACATGAAGATGCTAGTAGGTTGCACAATGAGGCAGCAGAAGATGAGGTTATTGGTACAAAAGTTTCCAATTTGCCCACTTCTGGGAGGAGAACAGCTGTGGCTGGAAAGTGGGGTTCAACCTTCTGGAAGGACTGTCAACCTATGCGCTCACAGGGTGGGTCTGATTCTGGTCAGGAAACTAAAAGTGGGTTGGACTACAGAAATGCTGTTGGATCTGAAGATAATTCTTCGGATGTCAGGGAAGATGGAATAGATTTTGAAGATGAGGGCAAGCCGAAAGTAAGCAAGGGACAACGGGGTCATTCTGACATCCCTGCAGATGAAATGTTATCTGATGAATATTATGAGCAGGATGGGGAAGAACAGAATGAATTGATGCGCTATAGAGGGTTTCATCACTCTGTTGGGTCAAATTCTAGGCCACAATCAAAGCCTAATTCAGTCAGCGACCGTGCACACTATAGAACTTCTAGAAATGATATTGAAGATTTTGAAGATGGTGATGATGCCAACGAAGATCTTGATGCTGCTGATGCTGATTATGAAGAAGAAGAAGATGAAGATGATCCTGATGATGCCGATTTTGAACCTGATTCTGGTGTTGGGAGTGGTCGCACTGCACAGAAGGATAATAATTGGAATGGTGAAGATTCTGATGATGATAATGATAGTGATGATGAGCTTGATGTTTCAGATGAAGATGATTCTTATTATGGAAAGAAACCTAAGAGTAGGTATCGTGGTAATGCTAAGTCTACAAGGGAACGCAAATCATATCATTCGTCTAGTCGGCAAAGAAGAGGGAAGTCATCGTTTGATGATGATGAGTCCTCAGCAGAGTACTCTGAAAGTGACAGTGACGAGGATTTTAAAAGTATTAAAAGGAAGGGTGTACATACCCGCAAGGGTAATGGCCGAAGAAATGTTACTGGACAAAATGGTGAGGTACGAACTTCTACTAGGTCTGTTAGGAAGGTCTCCTATGTTGAAAGTGATGGAAGTGATGAAGTTGAAGAAGGAAAGAAAAAGAAGTCCCAGAAGGAGGAGATTGAAGAAGAGGATGGTGACTATATTGAAAAGGTGCTTTGGCATCAGCCTAAGGGCATGGCTGAAGATGCTCTGAGGAACAATAAATCAACGGATCCTGTGCTATTAAGTCACTTGTTTGATTCTGAGCCAGATTGGAATCATATGGAGTTCTTGATAAAATGGAAAGGGCAGTCCCATTTGCACTGTCAGTGGAAATCAATTTCCGAGCTACAAAATCTTAGTGGTTTTAAGAAGGTTTTAAATTACACTAAAAAGGTGTTGGAAGATGCCAGGTACAGGAACACAATTTCACGTGAGGAGATTGAGGTTCACGATGTGAGCAAGGAAATGGACTTAGATCTCATCAAGCAGAATAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAGGCAAGATAGCTCTGGCGATGTGGGTCCGGAATTTCTAGTCAAGTGGCAAGGATTGTCTTATGCTGAAGCAACATGGGAGAAGGATGTAGATATTGCGTTTGCACAAGATGCTATCGATGAGTTCAAGGTTCGTGAAGCTGCAATGGCTGTACAAGGGAAAATGGTGGATCTCCAGCGCAAGAGGAGCAAAGGAAGTCTAAGGAAGCTCGAGGAACAGCCCGAATGGTTGAAGGGAGGAAAGCTTCGTGATTATCAGCTTGAGGGTTTGAATTTTCTTGTTAATAGTTGGAGGAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGTAAAACAGTTCAATCAGTTTCAATGCTTGGTTTTTTACAGAATGCTCAACAGATCCACGGGCCATTTCTTGTTGTTGTACCATTATCCACGTTGTCTAACTGGGCCAAAGAATTTAGGAAGTGGCTCCCTGATATGAATATTATTGTGTATGTTGGTACTCGTGCAAGCCGAGAGGTTTGTCAGCAGTATGAATTTAATAATGATAAAATAGTTGGCCGGCCTATTAAATTCAATGCACTATTGACGACGTATGAGGTGGTTTTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTAATGGTTGATGAAGCTCATAGATTAAAGAACAGTGAGGCACAGTTGTACACAACCCTCTTGGAATTTAGCACAAAGAACAAGTTGCTTATAACTGGTACTCCCTTGCAGAACAGCGTAGAGGAGTTATGGGCTCTGCTTCACTTCCTTGACCCTGATAAATTCAAGAATAAAGATGATTTTGTTCAGAATTACAAAAATCTCAGTTCCTTCAATGAGATTGAGCTTGCTAATCTGCATATGGAATTGAGGCCTCACATTCTTCGAAGAGTTATCAAGGATGTGGAAAAGTCTTTACCTCCTAAAATTGAACGGATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTATTATAAGTGGATTTTGGAACGCAACTTTCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCGTTGTTGAATATTGTGGTAGAATTGAAGAAATGTTGTAATCATCCCTTTCTGTTTGAAAGTGCTGACTATGGTTATGGTGGGGACTCAAGTAGTAAAGATGGCAGTAAATTGGAGAGGACTATTTTAAGCAGCGGAAAGTTGGTTATACTCGATAAGCTTCTTATGAAATTGCATGAAACAAAGCACCGTGTTCTCATATTTTCCCAGATGGTAAGAATGTTGGACATACTGGCGGAGTATATGTCAATTAGAGGTTTTCAATTTCAGCGGCTTGATGGAAGTACGAAGGCCGATTTACGCCACCAAGCAATGGAACATTTTAATGCGCCTGGTAGTGAAGACTTTTGCTTCCTCCTTTCAACTCGAGCAGGTGGCCTTGGTATCAACCTTGCCACAGCAGATACGGTTATAATATTCGACTCAGATTGGAATCCACAAAACGACCTGCAGGCAATGAGCAGAGCTCACAGAATAGGTCAACAAGAAGTTGTTAACATATATAGATTTGTCACAAGCAAAAGTGTCGAGGAAGATATCCTGGAGAGAGCTAAGAAAAAGATGGTGCTTGACCATTTGGTGATTCAAAAATTAAATGCAGAGGGTAGGTTGGAGAAGAAAGAATCTAAGAAAGGGACTTTATTTGACAAAAATGAGCTGTCTTCTATTCTACGATTTGGGGCTGAGGAACTTTTCAAGGAAGAAAAAAATGATGAAGAAAGCAAGAAAAGACTCTTAAGTATGGATATTGATGAGATTCTTGAAAGAGCAGAAAAGGTTGAAGAGAAGGAAACTGCAGAGGAAGGGAATGAATTGTTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGCTGAAGATGATGGTAGTTTTTGGAGTCGTTGGATAAAGCCTGAAGCAATATCCCAAGCCGAAGAAGCTCTTGCTCCTCGTGCCACTAGGAATACTAAGAGCTATGCAGAAGCTGATCAGCCTGATAGAAGTAATAAAAGAAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGACGGAAAGCAGATTATACAGTCACCTCAGTGCCAATGATTGACGGAGCATCTGCTCAAGTTAGGGGATGGTCTTCTGGGAACTTATCTAAGAGAGACGCATTGCGATTTTCTCGTGCTGTTATGAAATTTGGGAATGAGAGCCAAATTGCGTTGATAGTTGAGGAGGTCGGTGGTGCAGTTGCAGCAGCTTCAACTGATGCACAGGTTGAGCTTTTCAAGGCATTGATTGATGGCTGCACAGAAGCAGTGGAAGTGGGGAGTCTGGACCCGAAGGGGCCATTGCTGGATTTTTATGGTGTCCCTGTCAAAGCTGGTGATCTGATCAATCGTGTTCGTGAACTTCAACACCTGGCAAAACGCATTAGTCGATATGACGATCCCGTTGCTCAGTTTCGTGTCTTGATGTACCTCAAACCTTCAAATTGGTCGAAAGGTTGTGGCTGGAATCAAATTGATGATGCAAGATTGCTCATTGGAATATACTACCATGGCTTTGGAAATTGGGAAAAGATACGTTTAGATGAAAGGCTTGGCCTTACAAAGAAAATTGCTCCAGTGGAACTTCAACACCATGAAACATTTTTACCCCGTGCTCCGAATTTGAGAGATCGGGCCAACGCTCTTTTGGAAATGGATATTGCAGCTCTTAGTGGAAAGAATGCAAATGTTAAAGTGGGTCGTAAGGCTTCAAAGGAGAGGGAAGCTACTCGCAATGTTTCTTTAGCTCGCAGTGGGCCAAAGAAAGGAAAAACCAGTTCTTCTAGAGCTAATTTTCCAACGAACAAGGACAGACCACTGAAACCCCAGAAAGTTGAACCTTTAGTGAAAGAGGAGGGTGAAATGTCAGACGATGATGAAGTGTATGAGAAGTTTAAGGAGGAGAAATGGATGGAATGGTGTGAAGAGATGATGAAGGATGAAACTAAAACCTTAAATCGTCTTCAACGGTTGCAGACTATTAGTGCCAATCTCCCGAAGGAAAAGGTGCTTTCCAAGATTCGAAGTTATTTACAGCTTCTTGGACGAACGATAGATCAAATTGTTTTAGAAAATGAGGAGGAACCGTATGGACAAGATAGGATGAGCATGCGCTTGTGGAACTATGTTTCTACCTTTTCAAATCTGTCCGGGGAGAGACTTCATCAAATATACTCAAAACTTAAACAGCACCAGGATGTGGAGGCTGGCCCTTCCCACATGAATGGCTCTGCTTCTGGTCCCTTTGGCAGAGACAATGACCCTTCTCCCTTCTCCTTCCATTCGGACAGGCAAAGAGGTCCTGATACAGCGAAGTTTGAAGCATGGAAACGAAGGAGGAGAGGGGAAGCTGATAGCAATATCCAAGTTCAGGCCCCATCTCAAAGAAATATGAATAATGGTACCCGGTTAACTGATCCAAACTCATTAGGGATTCTTGGTGCAGGACCATCAGAAAATAAACGCACTGTGAATGAAAGGCCTTATAGGACACGGCAGACAGGTTATCGGCCAAGTGGGATTGGCACCATTGAACAAGACGCGGCTGAGCCGCTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SNF2_N

Protein Analysis

1748

Amino Acids

199.14

Weight (kDa)

5.6

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 530 - 582 9.1e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 620 - 787 3.8e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 624 - 908 7.1e-86 SNF2-related domain
DEAD PF00270 635 - 792 1.4e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 859 - 1083 2.3e-09 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 938 - 1051 6.4e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1363 - 1444 2.7e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1527 - 1621 2.1e-15 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2348, 2613, 3086
AarI CACCTGC 1 cut(s) 3035
AasI GACNNNNNNGTC 1 cut(s) 477
Acc36I ACCTGC 2 cut(s) 3035, 3129
Acc65I GGTACC 1 cut(s) 5084
AccB1I GGYRCC 2 cut(s) 5084, 5207
AccB7I CCANNNNNTGG 2 cut(s) 2986, 4882
AccI GTMKAC 1 cut(s) 935
AccII CGCG 1 cut(s) 5225
AccIII TCCGGA 1 cut(s) 1645
AciI CCGC 7 cut(s) 1081, 1612, 2808, 2909, 2944, 5225, 5234
AclWI GGATC 8 cut(s) 400, 740, 1316, 1329, 1794, 1999, 4076, 5093
AcoI YGGCCR 3 cut(s) 1093, 2167, 5192
AcuI CTGAAG 6 cut(s) 417, 1311, 1704, 2582, 3546, 3585
AcvI CACGTG 1 cut(s) 1523
AdeI CACNNNGTG 3 cut(s) 44, 335, 1546
AfiI CCNNNNNNNGG 9 cut(s) 260, 261, 776, 1222, 1506, 2986, 4448, 4882, 5053
AflII CTTAAG 1 cut(s) 3409
AjnI CCWGG 6 cut(s) 48, 1499, 3004, 3213, 4053, 4875
AjuI GAANNNNNNNTTGG 2 cut(s) 1932, 1964
AleI CACNNNNGTG 1 cut(s) 3731
Alw21I GWGCWC 3 cut(s) 637, 3142, 4300
Alw26I GTCTC 5 cut(s) 1162, 2595, 3792, 4834, 4929
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 8 cut(s) 400, 740, 1316, 1329, 1794, 1999, 4076, 5093
AlwNI CAGNNNCTG 1 cut(s) 3635
Ama87I CYCGRG 2 cut(s) 1826, 3039
Aor13HI TCCGGA 1 cut(s) 1645
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 8 cut(s) 212, 707, 1757, 2805, 3887, 3890, 3939, 4346
Asp700I GAANNNNTTC 2 cut(s) 84, 2571
Asp718I GGTACC 1 cut(s) 5084
AspA2I CCTAGG 1 cut(s) 64
AspLEI GCGC 5 cut(s) 327, 561, 1797, 3004, 4797
AsuC2I CCSGG 2 cut(s) 4834, 5089
AsuHPI GGTGA 8 cut(s) 692, 836, 1133, 1256, 3265, 3718, 4031, 4553
AsuII TTCGAA 2 cut(s) 2521, 4705
AvaI CYCGRG 2 cut(s) 1826, 3039
AvaII GGWCC 5 cut(s) 1643, 3970, 4922, 4982, 5129
AvrII CCTAGG 1 cut(s) 64
AxyI CCTNAGG 2 cut(s) 1278, 3684
BaeGI GKGCMC 1 cut(s) 637
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 1908, 1941
BamHI GGATCC 1 cut(s) 1321
BanI GGYRCC 2 cut(s) 5084, 5207
BanII GRGCYC 2 cut(s) 2392, 3142
BarI GAAGNNNNNNTAC 2 cut(s) 1090, 1122
BauI CACGAG 3 cut(s) 2112, 3600, 3816
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 4 cut(s) 3021, 3327, 3768, 4637
Bbv12I GWGCWC 3 cut(s) 637, 3142, 4300
BbvCI CCTCAGC 2 cut(s) 1012, 3357
BbvI GCAGC 8 cut(s) 224, 694, 1744, 2817, 3899, 3902, 3926, 4358
BceAI ACGGC 1 cut(s) 5192
BcgI CGANNNNNNTGC 4 cut(s) 3181, 3215, 4070, 4104
BciT130I CCWGG 6 cut(s) 50, 1501, 3006, 3215, 4055, 4877
BclI TGATCA 2 cut(s) 3637, 4026
BcnI CCSGG 2 cut(s) 4834, 5089
BcoDI GTCTC 5 cut(s) 1162, 2595, 3792, 4834, 4929
BfaI CTAG 9 cut(s) 65, 192, 597, 651, 966, 1140, 1655, 3609, 4467
BfmI CTRYAG 6 cut(s) 376, 495, 562, 640, 3122, 3468
BfrI CTTAAG 1 cut(s) 3409
BfuAI ACCTGC 2 cut(s) 3035, 3129
BglII AGATCT 2 cut(s) 697, 1567
BlnI CCTAGG 1 cut(s) 64
BlpI GCTNAGC 2 cut(s) 5229, 5237
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 8 cut(s) 50, 1501, 3006, 3215, 4055, 4834, 4877, 5089
Bme18I GGWCC 5 cut(s) 1643, 3970, 4922, 4982, 5129
BmeT110I CYCGRG 2 cut(s) 1826, 3039
BmrFI CCNGG 8 cut(s) 50, 1501, 3006, 3215, 4055, 4834, 4877, 5089
BmrI ACTGGG 1 cut(s) 2062
BmuI ACTGGG 1 cut(s) 2062
BpiI GAAGAC 4 cut(s) 3021, 3327, 3768, 4637
BplI GAGNNNNNCTC 2 cut(s) 3124, 3156
BpmI CTGGAG 3 cut(s) 1775, 3236, 4242
Bpu10I CCTNAGC 2 cut(s) 1012, 3357
Bpu1102I GCTNAGC 2 cut(s) 5229, 5237
Bpu14I TTCGAA 2 cut(s) 2521, 4705
BpuEI CTTGAG 3 cut(s) 1898, 2746, 3744
BpuMI CCSGG 2 cut(s) 4834, 5089
Bsa29I ATCGAT 1 cut(s) 1734
BsaAI YACGTR 1 cut(s) 1523
BsaBI GATNNNNATC 2 cut(s) 1365, 3105
BsaI GGTCTC 1 cut(s) 1162
BsaJI CCNNGG 5 cut(s) 64, 2008, 3052, 4192, 4833
BsaWI WCCGGW 1 cut(s) 1645
BsaXI ACNNNNNCTCC 6 cut(s) 2341, 2371, 2903, 2933, 4754, 4784
Bsc4I CCNNNNNNNGG 9 cut(s) 260, 261, 776, 1222, 1506, 2986, 4448, 4882, 5053
Bse118I RCCGGY 1 cut(s) 2169
Bse1I ACTGG 6 cut(s) 1114, 2057, 2356, 2910, 4259, 4458
Bse21I CCTNAGG 2 cut(s) 1278, 3684
Bse3DI GCAATG 6 cut(s) 1767, 2991, 3135, 3803, 3983, 4423
Bse8I GATNNNNATC 2 cut(s) 1365, 3105
BseAI TCCGGA 1 cut(s) 1645
BseBI CCWGG 6 cut(s) 50, 1501, 3006, 3215, 4055, 4877
BseCI ATCGAT 1 cut(s) 1734
BseDI CCNNGG 5 cut(s) 64, 2008, 3052, 4192, 4833
BseJI GATNNNNATC 2 cut(s) 1365, 3105
BseLI CCNNNNNNNGG 9 cut(s) 260, 261, 776, 1222, 1506, 2986, 4448, 4882, 5053
BseMI GCAATG 6 cut(s) 1767, 2991, 3135, 3803, 3983, 4423
BseNI ACTGG 6 cut(s) 1114, 2057, 2356, 2910, 4259, 4458
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 8 cut(s) 224, 694, 1744, 2817, 3899, 3902, 3926, 4358
BsgI GTGCAG 4 cut(s) 783, 3900, 3925, 5145
Bsh1236I CGCG 1 cut(s) 5225
Bsh1285I CGRYCG 1 cut(s) 631
BshNI GGYRCC 2 cut(s) 5084, 5207
BshVI ATCGAT 1 cut(s) 1734
BsiEI CGRYCG 1 cut(s) 631
BsiHKAI GWGCWC 3 cut(s) 637, 3142, 4300
BsiHKCI CYCGRG 2 cut(s) 1826, 3039
BsiSI CCGG 4 cut(s) 1646, 2170, 4833, 5089
BslFI GGGAC 7 cut(s) 483, 1198, 1387, 2771, 3322, 3989, 4908
BslI CCNNNNNNNGG 9 cut(s) 260, 261, 776, 1222, 1506, 2986, 4448, 4882, 5053
BsmAI GTCTC 5 cut(s) 1162, 2595, 3792, 4834, 4929
BsmBI CGTCTC 1 cut(s) 3792
BsmFI GGGAC 7 cut(s) 483, 1198, 1387, 2771, 3322, 3989, 4908
BsmI GAATGC 2 cut(s) 1999, 4369
Bso31I GGTCTC 1 cut(s) 1162
BsoBI CYCGRG 2 cut(s) 1826, 3039
Bsp119I TTCGAA 2 cut(s) 2521, 4705
Bsp1286I GDGCHC 4 cut(s) 637, 2392, 3142, 4300
Bsp13I TCCGGA 1 cut(s) 1645
Bsp1407I TGTACA 3 cut(s) 1072, 1768, 2304
Bsp1720I GCTNAGC 2 cut(s) 5229, 5237
Bsp19I CCATGG 1 cut(s) 4192
BspACI CCGC 7 cut(s) 1081, 1612, 2808, 2909, 2944, 5225, 5234
BspDI ATCGAT 1 cut(s) 1734
BspEI TCCGGA 1 cut(s) 1645
BspFNI CGCG 1 cut(s) 5225
BspMAI CTGCAG 3 cut(s) 499, 3126, 3472
BspMI ACCTGC 2 cut(s) 3035, 3129
BspPI GGATC 8 cut(s) 400, 740, 1316, 1329, 1794, 1999, 4076, 5093
BspT104I TTCGAA 2 cut(s) 2521, 4705
BspT107I GGYRCC 2 cut(s) 5084, 5207
BspTI CTTAAG 1 cut(s) 3409
BspTNI GGTCTC 1 cut(s) 1162
BsrDI GCAATG 6 cut(s) 1767, 2991, 3135, 3803, 3983, 4423
BsrFI RCCGGY 1 cut(s) 2169
BsrGI TGTACA 3 cut(s) 1072, 1768, 2304
BsrI ACTGG 6 cut(s) 1114, 2057, 2356, 2910, 4259, 4458
BssAI RCCGGY 1 cut(s) 2169
BssECI CCNNGG 5 cut(s) 64, 2008, 3052, 4192, 4833
BssSI CACGAG 3 cut(s) 2112, 3600, 3816
BssT1I CCWWGG 3 cut(s) 64, 3052, 4192
Bst2BI CACGAG 3 cut(s) 2112, 3600, 3816
Bst2UI CCWGG 6 cut(s) 50, 1501, 3006, 3215, 4055, 4877
Bst6I CTCTTC 6 cut(s) 973, 1230, 2518, 2601, 3450, 4602
BstAFI CTTAAG 1 cut(s) 3409
BstAPI GCANNNNNTGC 1 cut(s) 1727
BstAUI TGTACA 3 cut(s) 1072, 1768, 2304
BstBAI YACGTR 1 cut(s) 1523
BstBI TTCGAA 2 cut(s) 2521, 4705
BstC8I GCNNGC 8 cut(s) 452, 2119, 2171, 2484, 3126, 4432, 4793, 4891
BstDSI CCRYGG 2 cut(s) 2008, 4192
BstFNI CGCG 1 cut(s) 5225
BstHHI GCGC 5 cut(s) 327, 561, 1797, 3004, 4797
BstMAI GTCTC 5 cut(s) 1162, 2595, 3792, 4834, 4929
BstMCI CGRYCG 1 cut(s) 631
BstMWI GCNNNNNNNGC 8 cut(s) 713, 1295, 1727, 1763, 1803, 3587, 3602, 3887
BstNI CCWGG 6 cut(s) 50, 1501, 3006, 3215, 4055, 4877
BstNSI RCATGY 1 cut(s) 4795
BstSCI CCNGG 8 cut(s) 48, 1499, 3004, 3213, 4053, 4832, 4875, 5087
BstSFI CTRYAG 6 cut(s) 376, 495, 562, 640, 3122, 3468
BstSLI GKGCMC 1 cut(s) 637
BstUI CGCG 1 cut(s) 5225
BstV1I GCAGC 8 cut(s) 224, 694, 1744, 2817, 3899, 3902, 3926, 4358
BstV2I GAAGAC 4 cut(s) 3021, 3327, 3768, 4637
BstX2I RGATCY 6 cut(s) 392, 697, 1321, 1567, 1786, 2004
BstXI CCANNNNNNTGG 2 cut(s) 4199, 4907
BstYI RGATCY 6 cut(s) 392, 697, 1321, 1567, 1786, 2004
Bsu15I ATCGAT 1 cut(s) 1734
Bsu36I CCTNAGG 2 cut(s) 1278, 3684
BsuTUI ATCGAT 1 cut(s) 1734
BtgI CCRYGG 2 cut(s) 2008, 4192
BtgZI GCGATG 1 cut(s) 1650
BtsI GCAGTG 3 cut(s) 795, 3957, 4441
BveI ACCTGC 2 cut(s) 3035, 3129
Cac8I GCNNGC 8 cut(s) 452, 2119, 2171, 2484, 3126, 4432, 4793, 4891
CaiI CAGNNNCTG 1 cut(s) 3635
CfoI GCGC 5 cut(s) 327, 561, 1797, 3004, 4797
Cfr10I RCCGGY 1 cut(s) 2169
ClaI ATCGAT 1 cut(s) 1734
CseI GACGC 2 cut(s) 3809, 5231
DraI TTTAAA 3 cut(s) 1051, 1470, 2217
DraIII CACNNNGTG 3 cut(s) 44, 335, 1546
DrdI GACNNNNNNGTC 1 cut(s) 477
DseDI GACNNNNNNGTC 1 cut(s) 477
EaeI YGGCCR 3 cut(s) 1093, 2167, 5192
Eam1104I CTCTTC 6 cut(s) 973, 1230, 2518, 2601, 3450, 4602
EarI CTCTTC 6 cut(s) 973, 1230, 2518, 2601, 3450, 4602
EciI GGCGGA 1 cut(s) 2924
Ecl136II GAGCTC 1 cut(s) 3140
Eco130I CCWWGG 3 cut(s) 64, 3052, 4192
Eco147I AGGCCT 2 cut(s) 2509, 5164
Eco24I GRGCYC 2 cut(s) 2392, 3142
Eco31I GGTCTC 1 cut(s) 1162
Eco32I GATATC 2 cut(s) 145, 3211
Eco47I GGWCC 5 cut(s) 1643, 3970, 4922, 4982, 5129
Eco53kI GAGCTC 1 cut(s) 3140
Eco57I CTGAAG 6 cut(s) 417, 1311, 1704, 2582, 3546, 3585
Eco72I CACGTG 1 cut(s) 1523
Eco81I CCTNAGG 2 cut(s) 1278, 3684
Eco88I CYCGRG 2 cut(s) 1826, 3039
EcoICRI GAGCTC 1 cut(s) 3140
EcoO109I RGGNCCY 2 cut(s) 4982, 5056
EcoRI GAATTC 1 cut(s) 84
EcoRII CCWGG 6 cut(s) 48, 1499, 3004, 3213, 4053, 4875
EcoRV GATATC 2 cut(s) 145, 3211
EcoT14I CCWWGG 3 cut(s) 64, 3052, 4192
EcoT38I GRGCYC 2 cut(s) 2392, 3142
ErhI CCWWGG 3 cut(s) 64, 3052, 4192
Esp3I CGTCTC 1 cut(s) 3792
FalI AAGNNNNNCTT 2 cut(s) 3576, 3608
FaqI GGGAC 7 cut(s) 483, 1198, 1387, 2771, 3322, 3989, 4908
FauI CCCGC 1 cut(s) 1088
FauNDI CATATG 2 cut(s) 1371, 2496
FbaI TGATCA 2 cut(s) 3637, 4026
FblI GTMKAC 1 cut(s) 935
FriOI GRGCYC 2 cut(s) 2392, 3142
FseI GGCCGGCC 1 cut(s) 2173
FspBI CTAG 9 cut(s) 65, 192, 597, 651, 966, 1140, 1655, 3609, 4467
GlaI GCGC 5 cut(s) 326, 560, 1796, 3003, 4796
GsuI CTGGAG 3 cut(s) 1775, 3236, 4242
HapII CCGG 4 cut(s) 1646, 2170, 4833, 5089
HgaI GACGC 2 cut(s) 3809, 5231
HhaI GCGC 5 cut(s) 327, 561, 1797, 3004, 4797
Hin6I GCGC 5 cut(s) 325, 559, 1795, 3002, 4795
HinP1I GCGC 5 cut(s) 325, 559, 1795, 3002, 4795
HincII GTYRAC 4 cut(s) 317, 3155, 3169, 5094
HindII GTYRAC 4 cut(s) 317, 3155, 3169, 5094
HindIII AAGCTT 2 cut(s) 1859, 2831
HpaI GTTAAC 2 cut(s) 3169, 5094
HpaII CCGG 4 cut(s) 1646, 2170, 4833, 5089
HphI GGTGA 8 cut(s) 692, 836, 1133, 1256, 3265, 3718, 4031, 4553
Hpy99I CGWCG 1 cut(s) 2203
HpyCH4IV ACGT 4 cut(s) 1522, 2042, 2201, 4219
HpyF10VI GCNNNNNNNGC 8 cut(s) 713, 1295, 1727, 1763, 1803, 3587, 3602, 3887
HpySE526I ACGT 4 cut(s) 1522, 2042, 2201, 4219
HspAI GCGC 5 cut(s) 325, 559, 1795, 3002, 4795
Kpn2I TCCGGA 1 cut(s) 1645
KpnI GGTACC 1 cut(s) 5088
KroI GCCGGC 1 cut(s) 2169
KroNI GCCGGC 1 cut(s) 2171
Ksp22I TGATCA 2 cut(s) 3637, 4026
KspAI GTTAAC 2 cut(s) 3169, 5094
LmnI GCTCC 7 cut(s) 98, 1803, 2082, 3601, 3749, 4261, 4303
Lsp1109I GCAGC 8 cut(s) 224, 694, 1744, 2817, 3899, 3902, 3926, 4358
MaeI CTAG 9 cut(s) 65, 192, 597, 651, 966, 1140, 1655, 3609, 4467
MaeII ACGT 4 cut(s) 1522, 2042, 2201, 4219
MaeIII GTNAC 7 cut(s) 1031, 1037, 1105, 1244, 1337, 3184, 3724
MflI RGATCY 6 cut(s) 392, 697, 1321, 1567, 1786, 2004
MhlI GDGCHC 4 cut(s) 637, 2392, 3142, 4300
MlyI GAGTC 7 cut(s) 1016, 2753, 3089, 3399, 3556, 3702, 3973
MmeI TCCRAC 8 cut(s) 351, 370, 1470, 1886, 2877, 3264, 3533, 4505
MroI TCCGGA 1 cut(s) 1645
MroNI GCCGGC 1 cut(s) 2169
MroXI GAANNNNTTC 2 cut(s) 84, 2571
MslI CAYNNNNRTG 5 cut(s) 186, 2997, 3731, 4878, 4905
MspA1I CMGCKG 4 cut(s) 275, 2808, 2944, 5236
MspCI CTTAAG 1 cut(s) 3409
MspI CCGG 4 cut(s) 1646, 2170, 4833, 5089
MspR9I CCNGG 8 cut(s) 50, 1501, 3006, 3215, 4055, 4834, 4877, 5089
Mva1269I GAATGC 2 cut(s) 1999, 4369
MvaI CCWGG 6 cut(s) 50, 1501, 3006, 3215, 4055, 4877
MvnI CGCG 1 cut(s) 5225
MwoI GCNNNNNNNGC 8 cut(s) 713, 1295, 1727, 1763, 1803, 3587, 3602, 3887
NaeI GCCGGC 1 cut(s) 2171
NciI CCSGG 2 cut(s) 4834, 5089
NcoI CCATGG 1 cut(s) 4192
NdeI CATATG 2 cut(s) 1371, 2496
NgoMIV GCCGGC 1 cut(s) 2169
NmeAIII GCCGAG 1 cut(s) 2147
NmuCI GTSAC 6 cut(s) 1031, 1037, 1244, 1337, 3184, 3724
NspI RCATGY 1 cut(s) 4795
NspV TTCGAA 2 cut(s) 2521, 4705
OliI CACNNNNGTG 1 cut(s) 3731
PaeI GCATGC 1 cut(s) 4795
PaeR7I CTCGAG 2 cut(s) 1826, 3039
PaqCI CACCTGC 1 cut(s) 3035
PceI AGGCCT 2 cut(s) 2509, 5164
PcsI WCGNNNNNNNCGW 2 cut(s) 2119, 4069
PctI GAATGC 2 cut(s) 1999, 4369
PdiI GCCGGC 1 cut(s) 2171
PdmI GAANNNNTTC 2 cut(s) 84, 2571
PflMI CCANNNNNTGG 2 cut(s) 2986, 4882
PfoI TCCNGGA 2 cut(s) 48, 3213
PleI GAGTC 7 cut(s) 1015, 2753, 3089, 3399, 3555, 3701, 3972
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 7 cut(s) 1015, 2753, 3089, 3399, 3555, 3701, 3972
Ppu21I YACGTR 1 cut(s) 1523
PpuMI RGGWCCY 1 cut(s) 4982
PsiI TTATAA 3 cut(s) 2348, 2613, 3086
Psp124BI GAGCTC 1 cut(s) 3142
Psp5II RGGWCCY 1 cut(s) 4982
Psp6I CCWGG 6 cut(s) 48, 1499, 3004, 3213, 4053, 4875
PspCI CACGTG 1 cut(s) 1523
PspGI CCWGG 6 cut(s) 48, 1499, 3004, 3213, 4053, 4875
PspPPI RGGWCCY 1 cut(s) 4982
PspXI VCTCGAGB 2 cut(s) 1826, 3039
PstI CTGCAG 3 cut(s) 499, 3126, 3472
PstNI CAGNNNCTG 1 cut(s) 3635
PsuI RGATCY 6 cut(s) 392, 697, 1321, 1567, 1786, 2004
PvuII CAGCTG 1 cut(s) 275
RigI GGCCGGCC 1 cut(s) 2173
RseI CAYNNNNRTG 5 cut(s) 186, 2997, 3731, 4878, 4905
SacI GAGCTC 1 cut(s) 3142
SbfI CCTGCAGG 1 cut(s) 3126
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 7 cut(s) 1016, 2753, 3089, 3399, 3556, 3702, 3973
ScrFI CCNGG 8 cut(s) 50, 1501, 3006, 3215, 4055, 4834, 4877, 5089
SdaI CCTGCAGG 1 cut(s) 3126
SduI GDGCHC 4 cut(s) 637, 2392, 3142, 4300
SfcI CTRYAG 6 cut(s) 376, 495, 562, 640, 3122, 3468
Sfr274I CTCGAG 2 cut(s) 1826, 3039
SfuI TTCGAA 2 cut(s) 2521, 4705
SinI GGWCC 5 cut(s) 1643, 3970, 4922, 4982, 5129
SlaI CTCGAG 2 cut(s) 1826, 3039
SmiMI CAYNNNNRTG 5 cut(s) 186, 2997, 3731, 4878, 4905
SmlI CTYRAG 6 cut(s) 1826, 1877, 2761, 3039, 3409, 3759
SmoI CTYRAG 6 cut(s) 1826, 1877, 2761, 3039, 3409, 3759
SphI GCATGC 1 cut(s) 4795
Sse8387I CCTGCAGG 1 cut(s) 3126
SseBI AGGCCT 2 cut(s) 2509, 5164
SsiI CCGC 7 cut(s) 1081, 1612, 2808, 2909, 2944, 5225, 5234
SspI AATATT 4 cut(s) 521, 2092, 2686, 3090
SspMI CTAG 9 cut(s) 65, 192, 597, 651, 966, 1140, 1655, 3609, 4467
SstI GAGCTC 1 cut(s) 3142
StuI AGGCCT 2 cut(s) 2509, 5164
StyD4I CCNGG 8 cut(s) 48, 1499, 3004, 3213, 4053, 4832, 4875, 5087
StyI CCWWGG 3 cut(s) 64, 3052, 4192
TaiI ACGT 4 cut(s) 1525, 2045, 2204, 4222
TatI WGTACW 4 cut(s) 1020, 1072, 1768, 2304
TauI GCSGC 3 cut(s) 2947, 5228, 5236
TseFI GTSAC 6 cut(s) 1031, 1037, 1244, 1337, 3184, 3724
TseI GCWGC 8 cut(s) 212, 707, 1757, 2805, 3887, 3890, 3939, 4346
Tsp45I GTSAC 6 cut(s) 1031, 1037, 1244, 1337, 3184, 3724
TspGWI ACGGA 4 cut(s) 1334, 2584, 3721, 3762
Van91I CCANNNNNTGG 2 cut(s) 2986, 4882
Vha464I CTTAAG 1 cut(s) 3409
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 5 cut(s) 1643, 3970, 4922, 4982, 5129
XbaI TCTAGA 2 cut(s) 650, 4466
XceI RCATGY 1 cut(s) 4795
XcmI CCANNNNNNNNNTGG 1 cut(s) 4327
XhoI CTCGAG 2 cut(s) 1826, 3039
XmaJI CCTAGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 935
XmnI GAANNNNTTC 2 cut(s) 84, 2571
XspI CTAG 9 cut(s) 65, 192, 597, 651, 966, 1140, 1655, 3609, 4467
ZrmI AGTACT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.