pycom03g13410

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012741
Physical Location & Seq
Forward (+)
14420776 .. 14421912
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGTGGTTTTCTGATTATGTCTTCTTGGAAAGAAGATGGACAAAAGATGAAAGCACATTGAAGTCAGGTTTTCGAAGACTAGAGGACTTCCCCATGCCTTTTTGGTTGGCTCTCTTTGTAGAAGGAACACGATTTACGCAGGCAAAGCTAATGGTAGCTCAAGAGTTTGCAGCTTCTAGAGGGTTGCCTATTCCTAGGAACGTCTTGCTTCCACGGACGAAGGTGGCATTTCGTGCTGGCCTGGTGTGCCGTTGCATCCTTATGCATGATCTTGTTTATGGGACTTATGCTTCTGCTAAAATTCTAGGCCATAACTTCTGGAAAAATGCAATGGGGCTGACATATTATCAACTGTATCTGCTTCCATGTAGAACTGAGGCCAACAGTTGTGGGAATAGGTTCGGAAATAATGTTACTAACTATAAGGAATTCCGATTGTTGCAGTTAAAATTACAAATCAGGAGACACTCAATGCAGGAGTTGCCAGAAACGGCTGATGGAATTGGACAATGGTGTAAAGATGTGTTTGTTACCAAGGTATAG

Protein Analysis

181

Amino Acids

21.03

Weight (kDa)

9.61

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 149 - 179 1.5e-07 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 300, 428
AgsI TTSAA 1 cut(s) 61
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 3 cut(s) 148, 158, 173
AluI AGCT 3 cut(s) 148, 158, 173
Alw26I GTCTC 1 cut(s) 457
AoxI GGCC 3 cut(s) 238, 307, 378
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 300, 428
Asp700I GAANNNNTTC 1 cut(s) 398
AspA2I CCTAGG 1 cut(s) 194
AsuII TTCGAA 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 194
BbsI GAAGAC 2 cut(s) 13, 82
BbvI GCAGC 1 cut(s) 182
BccI CCATC 2 cut(s) 30, 491
BceAI ACGGC 2 cut(s) 234, 507
BciT130I CCWGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 4 cut(s) 80, 177, 195, 305
BisI GCNGC 1 cut(s) 171
BlnI CCTAGG 1 cut(s) 194
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 13, 82
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 194, 212, 534
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 3 cut(s) 194, 212, 534
BseGI GGATG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 182
BshFI GGCC 3 cut(s) 240, 309, 380
BslFI GGGAC 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 3 cut(s) 240, 309, 380
Bsp119I TTCGAA 1 cut(s) 73
Bsp143I GATC 1 cut(s) 268
BspANI GGCC 3 cut(s) 240, 309, 380
BspCNI CTCAG 1 cut(s) 367
BspT104I TTCGAA 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 336
BssECI CCNNGG 3 cut(s) 194, 212, 534
BssMI GATC 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 194, 534
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 354, 386
BstAPI GCANNNNNTGC 2 cut(s) 233, 481
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 141, 238
BstDEI CTNAG 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 255
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 4 cut(s) 145, 233, 246, 481
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 2 cut(s) 13, 82
BsuRI GGCC 3 cut(s) 240, 309, 380
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 141, 238
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 3 cut(s) 94, 266, 366
CviJI RGCY 9 cut(s) 110, 148, 158, 173, 240, 309, 337, 380, 494
CviKI_1 RGCY 9 cut(s) 110, 148, 158, 173, 240, 309, 337, 380, 494
DdeI CTNAG 1 cut(s) 375
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 194, 534
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 2 cut(s) 194, 534
EcoT22I ATGCAT 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 194, 534
FaeI CATG 3 cut(s) 97, 269, 369
FaqI GGGAC 1 cut(s) 295
FatI CATG 3 cut(s) 93, 265, 365
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 4 cut(s) 80, 177, 195, 305
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 3 cut(s) 240, 309, 380
Hin1II CATG 3 cut(s) 97, 269, 369
Hpy188I TCNGA 3 cut(s) 13, 404, 434
Hpy188III TCNNGA 4 cut(s) 161, 177, 319, 460
HpyAV CCTTC 2 cut(s) 116, 214
HpyCH4III ACNGT 2 cut(s) 354, 386
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 6 cut(s) 170, 255, 265, 329, 442, 475
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 233, 246, 481
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 3 cut(s) 97, 269, 369
Kzo9I GATC 1 cut(s) 268
LpnPI CCDG 9 cut(s) 51, 125, 222, 227, 254, 304, 445, 461, 498
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 264
MaeI CTAG 4 cut(s) 80, 177, 195, 305
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 412, 529
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 3 cut(s) 13, 45, 87
MluCI AATT 4 cut(s) 300, 428, 449, 501
MnlI CCTC 3 cut(s) 76, 173, 370
Mph1103I ATGCAT 1 cut(s) 267
MroXI GAANNNNTTC 1 cut(s) 398
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 242
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 4 cut(s) 145, 233, 246, 481
NdeII GATC 1 cut(s) 268
NlaIII CATG 3 cut(s) 97, 269, 369
NsiI ATGCAT 1 cut(s) 267
NspV TTCGAA 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 398
PkrI GCNGC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PsrI GAACNNNNNNTAC 2 cut(s) 118, 150
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 242
SetI ASST 8 cut(s) 70, 150, 160, 175, 204, 225, 401, 540
SfaNI GCATC 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 4 cut(s) 300, 428, 449, 501
SspMI CTAG 4 cut(s) 80, 177, 195, 305
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 2 cut(s) 194, 534
TaaI ACNGT 2 cut(s) 354, 386
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 73
TasI AATT 4 cut(s) 300, 428, 449, 501
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 229
XapI RAATTY 2 cut(s) 300, 428
XbaI TCTAGA 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 4 cut(s) 80, 177, 195, 305
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.