pycom04g00610

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008769
Physical Location & Seq
Reverse (-)
587283 .. 594813
7531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00610.1

Sequence Viewer

Length: 1356 bp
ATGATGCTGCGTCGCGCCCTTCATGCCCAAAGGCTTCCTCTGCTCTTCCACAATCGTCCCAGAGTGAAGCCATGTGAGCTGTGGGCGTCCCCATTTGAAGCCCTTTTCTCCACTCACGTTGTCGGTGACAAACCGATTCTGGTTCGTGATTTCATTCATTCGGCATTATATGATCCGAAACATGGGTATTTCTCTCAAAGATCGAAATCGGTTGGGGTGCTTCAGGGAGCAATTAAGTTCAATCAGCTCGAAGGTAGGAAAGCTTATATGAAATACTTGGAGAAAATTTACAAAGAAAGTGATGTATCTTGGTTTACTCCTGTGGAAATTTTTAAGCCTTGGTATGCGCATGGGATTGCTGAAGCCATAATGCGTACCGCTAACCTTTCAGTTCCTCTAAGGATATATGAAATTGGTGGTGGATCAGGAACTTGTGCAAAGGGAATCATGGATTACATCATGCTGAATGCACCCCCAAGAGTTTACAACAATATGACTTACATCTCAGTTGAAGTCAGTCCCTCACTGGCTGAGATACAACGAGAAACTGTTGGAGAAATCCGTAGTCACTTATCAAAGTTTCGAGTAGAATGTCGTGATGCTACCGACCAGAGTGGATGGGGAGATGTGGAGCACCAACCATGTTGGGTAATAATGCTTGAGGTACTTGATAATCTTCCGCATGATCTTATCTATTCAGAAAATCAAGTTTCCCCATGGATGGAAGTATGGGTAGAGAAGCAACAGGATAGGGAAACACCTTCTGAATTGTATAAGCCATTGCAAGACTCATTAATTAAGAGATGTCTGGAGATCATCGATTTAGACAAAAAACATGATTCTCAGAGCAGTGCAATACTAAAAGCAAAAGGCGTTTGGTCTAAAGTCTTTCCAAAACCTCGAAGATGTTGGCTACCAACCGGTTGCTTGAAACTGCTTGAAGTTTTACATGGAGTGCTACCAAAGATGTCTTTAATTGCTTCAGACTTTAGTTACCTACCTGATGTAAAAATACTTGGTGAAAGAGCTCCATTGGTTTCAACCAAGAAAGATGGCAGCAGCTCGGATTATAACAGTTATCTGGATGCAAGGGGAGATGCTGATATATTTTTCCCCACTGAATTTTGGCTTTTGGAACGTATTGACCATTACTGTTCTGGATGGCTGAAAATTCAGAAAGATAAGTCGTCCAAGGAAGGGAAGAAGAGGCGAGCAATTACGCTTGATACGTCGTCATTCATGGAAGAGTTTGGCTTGCCCTCAAAGACAAGAACCAAGGACGGATACAATCCACTCTTGGATGACTTCAAGAATACGAAATTTTATCTCAGTGTTCCAACACATAATACCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

452

Amino Acids

51.82

Weight (kDa)

8.56

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 123 - 387 7.4e-31 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1071
Acc16I TGCGCA 1 cut(s) 348
AccII CGCG 1 cut(s) 15
AciI CCGC 2 cut(s) 378, 680
AclWI GGATC 2 cut(s) 167, 430
AcsI RAATTY 5 cut(s) 285, 327, 1121, 1170, 1319
AcuI CTGAAG 3 cut(s) 206, 381, 966
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 2 cut(s) 376, 666
AfiI CCNNNNNNNGG 3 cut(s) 182, 721, 1197
AgeI ACCGGT 1 cut(s) 920
AgsI TTSAA 7 cut(s) 98, 241, 512, 931, 941, 1041, 1309
AluBI AGCT 5 cut(s) 79, 247, 263, 1028, 1062
AluI AGCT 5 cut(s) 79, 247, 263, 1028, 1062
Alw21I GWGCWC 2 cut(s) 636, 1030
AlwI GGATC 2 cut(s) 167, 430
ApeKI GCWGC 3 cut(s) 7, 1056, 1059
ApoI RAATTY 5 cut(s) 285, 327, 1121, 1170, 1319
AseI ATTAAT 1 cut(s) 794
AsiGI ACCGGT 1 cut(s) 920
AspLEI GCGC 2 cut(s) 17, 349
AsuHPI GGTGA 2 cut(s) 137, 1031
BanII GRGCYC 1 cut(s) 1030
Bbv12I GWGCWC 2 cut(s) 636, 1030
BbvI GCAGC 2 cut(s) 1068, 1071
BccI CCATC 4 cut(s) 612, 715, 1046, 1155
BciVI GTATCC 1 cut(s) 1277
BfuI GTATCC 1 cut(s) 1277
BisI GCNGC 3 cut(s) 8, 1057, 1060
BlsI GCNGC 3 cut(s) 9, 1058, 1061
BmrI ACTGGG 1 cut(s) 1345
BmsI GCATC 3 cut(s) 589, 1075, 1087
BmuI ACTGGG 1 cut(s) 1345
BpmI CTGGAG 1 cut(s) 830
BpuEI CTTGAG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 819
BsaBI GATNNNNATC 1 cut(s) 205
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 338, 716, 1191, 1275
BsaWI WCCGGW 1 cut(s) 920
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 721, 1197
Bse118I RCCGGY 1 cut(s) 920
Bse1I ACTGG 2 cut(s) 531, 1351
Bse3DI GCAATG 1 cut(s) 779
Bse8I GATNNNNATC 1 cut(s) 205
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 4 cut(s) 338, 716, 1191, 1275
BseGI GGATG 5 cut(s) 623, 726, 1090, 1166, 1306
BseJI GATNNNNATC 1 cut(s) 205
BseLI CCNNNNNNNGG 3 cut(s) 182, 721, 1197
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 4 cut(s) 519, 522, 857, 1342
BseNI ACTGG 2 cut(s) 531, 1351
BseXI GCAGC 2 cut(s) 1068, 1071
Bsh1236I CGCG 1 cut(s) 15
BshTI ACCGGT 1 cut(s) 920
BshVI ATCGAT 1 cut(s) 819
BsiHKAI GWGCWC 2 cut(s) 636, 1030
BsiSI CCGG 1 cut(s) 921
BslFI GGGAC 3 cut(s) 42, 73, 504
BslI CCNNNNNNNGG 3 cut(s) 182, 721, 1197
BsmFI GGGAC 3 cut(s) 42, 73, 504
BsmI GAATGC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 636, 1030
Bsp143I GATC 5 cut(s) 172, 200, 422, 685, 813
Bsp19I CCATGG 1 cut(s) 716
BspACI CCGC 2 cut(s) 378, 680
BspCNI CTCAG 4 cut(s) 518, 523, 856, 1341
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 1 cut(s) 15
BspPI GGATC 2 cut(s) 167, 430
BspQI GCTCTTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 920
BsrI ACTGG 2 cut(s) 531, 1351
BssAI RCCGGY 1 cut(s) 920
BssECI CCNNGG 4 cut(s) 338, 716, 1191, 1275
BssMI GATC 5 cut(s) 172, 200, 422, 685, 813
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 4 cut(s) 338, 716, 1191, 1275
Bst4CI ACNGT 3 cut(s) 550, 1076, 1154
Bst6I CTCTTC 3 cut(s) 50, 1199, 1239
BstACI GRCGYC 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 1212, 1256
BstDEI CTNAG 5 cut(s) 398, 505, 531, 843, 1328
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 5 cut(s) 623, 726, 1090, 1166, 1306
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 2 cut(s) 17, 349
BstKTI GATC 5 cut(s) 175, 203, 425, 688, 816
BstMBI GATC 5 cut(s) 172, 200, 422, 685, 813
BstMWI GCNNNNNNNGC 3 cut(s) 23, 40, 76
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 1068, 1071
Bsu15I ATCGAT 1 cut(s) 819
BsuI GTATCC 1 cut(s) 1277
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 1 cut(s) 716
BtsCI GGATG 5 cut(s) 623, 726, 1090, 1166, 1306
BtsI GCAGTG 1 cut(s) 856
BtsIMutI CAGTG 4 cut(s) 524, 856, 1116, 1336
Cac8I GCNNGC 2 cut(s) 1212, 1256
CfoI GCGC 2 cut(s) 17, 349
Cfr10I RCCGGY 1 cut(s) 920
ClaI ATCGAT 1 cut(s) 819
CseI GACGC 1 cut(s) 75
Csp6I GTAC 2 cut(s) 375, 665
CspAI ACCGGT 1 cut(s) 920
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviQI GTAC 2 cut(s) 375, 665
DdeI CTNAG 5 cut(s) 398, 505, 531, 843, 1328
DpnI GATC 5 cut(s) 174, 202, 424, 687, 815
DpnII GATC 5 cut(s) 172, 200, 422, 685, 813
Eam1104I CTCTTC 3 cut(s) 50, 1199, 1239
EarI CTCTTC 3 cut(s) 50, 1199, 1239
Ecl136II GAGCTC 1 cut(s) 1028
Eco130I CCWWGG 4 cut(s) 338, 716, 1191, 1275
Eco24I GRGCYC 1 cut(s) 1030
Eco53kI GAGCTC 1 cut(s) 1028
Eco57I CTGAAG 3 cut(s) 206, 381, 966
EcoICRI GAGCTC 1 cut(s) 1028
EcoT14I CCWWGG 4 cut(s) 338, 716, 1191, 1275
EcoT38I GRGCYC 1 cut(s) 1030
ErhI CCWWGG 4 cut(s) 338, 716, 1191, 1275
FaqI GGGAC 3 cut(s) 42, 73, 504
Fnu4HI GCNGC 3 cut(s) 8, 1057, 1060
FokI GGATG 5 cut(s) 630, 733, 1097, 1173, 1313
FriOI GRGCYC 1 cut(s) 1030
Fsp4HI GCNGC 3 cut(s) 8, 1057, 1060
FspAI RTGCGCAY 1 cut(s) 348
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 2 cut(s) 16, 348
GluI GCNGC 3 cut(s) 8, 1057, 1060
GsuI CTGGAG 1 cut(s) 830
HapII CCGG 1 cut(s) 921
HgaI GACGC 1 cut(s) 75
HhaI GCGC 2 cut(s) 17, 349
Hin1I GRCGYC 1 cut(s) 86
Hin6I GCGC 2 cut(s) 15, 347
HinP1I GCGC 2 cut(s) 15, 347
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 4 cut(s) 136, 444, 788, 839
HpaII CCGG 1 cut(s) 921
HphI GGTGA 2 cut(s) 137, 1031
Hpy166II GTNNAC 2 cut(s) 315, 484
Hpy188I TCNGA 7 cut(s) 177, 700, 766, 846, 985, 1066, 1176
Hpy188III TCNNGA 7 cut(s) 146, 426, 596, 809, 1082, 1158, 1309
Hpy8I GTNNAC 2 cut(s) 315, 484
Hpy99I CGWCG 2 cut(s) 15, 1234
HpyAV CCTTC 4 cut(s) 29, 245, 771, 1190
HpyCH4III ACNGT 3 cut(s) 550, 1076, 1154
HpyCH4IV ACGT 3 cut(s) 117, 1138, 1229
HpyCH4V TGCA 5 cut(s) 437, 470, 784, 854, 1088
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 40, 76
HpyF3I CTNAG 5 cut(s) 398, 505, 531, 843, 1328
HpySE526I ACGT 3 cut(s) 117, 1138, 1229
Hsp92I GRCGYC 1 cut(s) 86
HspAI GCGC 2 cut(s) 15, 347
Kzo9I GATC 5 cut(s) 172, 200, 422, 685, 813
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 3 cut(s) 227, 631, 1033
Lsp1109I GCAGC 2 cut(s) 1068, 1071
LweI GCATC 3 cut(s) 589, 1075, 1087
MaeII ACGT 3 cut(s) 117, 1138, 1229
MaeIII GTNAC 3 cut(s) 125, 566, 992
MalI GATC 5 cut(s) 174, 202, 424, 687, 815
MboI GATC 5 cut(s) 172, 200, 422, 685, 813
MboII GAAGA 6 cut(s) 37, 668, 915, 1213, 1216, 1256
MhlI GDGCHC 2 cut(s) 636, 1030
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 7 cut(s) 48, 405, 532, 655, 909, 1200, 1270
MseI TTAA 5 cut(s) 234, 333, 794, 798, 974
MspI CCGG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 472
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 23, 40, 76
NcoI CCATGG 1 cut(s) 716
NdeII GATC 5 cut(s) 172, 200, 422, 685, 813
NmuCI GTSAC 2 cut(s) 125, 566
NsbI TGCGCA 1 cut(s) 348
PacI TTAATTAA 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 1226
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 3 cut(s) 136, 444, 839
PinAI ACCGGT 1 cut(s) 920
PkrI GCNGC 3 cut(s) 9, 1058, 1061
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 794
PsiI TTATAA 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 1030
RsaI GTAC 2 cut(s) 376, 666
RsaNI GTAC 2 cut(s) 375, 665
SacI GAGCTC 1 cut(s) 1030
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 5 cut(s) 234, 333, 794, 798, 974
SatI GCNGC 3 cut(s) 8, 1057, 1060
Sau3AI GATC 5 cut(s) 172, 200, 422, 685, 813
SchI GAGTC 1 cut(s) 782
SduI GDGCHC 2 cut(s) 636, 1030
SfaNI GCATC 3 cut(s) 589, 1075, 1087
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SsiI CCGC 2 cut(s) 378, 680
SstI GAGCTC 1 cut(s) 1030
StyI CCWWGG 4 cut(s) 338, 716, 1191, 1275
TaaI ACNGT 3 cut(s) 550, 1076, 1154
TaiI ACGT 3 cut(s) 120, 1141, 1232
TaqI TCGA 5 cut(s) 203, 249, 583, 819, 901
TfiI GAWTC 3 cut(s) 136, 444, 839
Tru1I TTAA 5 cut(s) 234, 333, 794, 798, 974
Tru9I TTAA 5 cut(s) 234, 333, 794, 798, 974
TscAI CASTG 4 cut(s) 531, 856, 1123, 1336
TseFI GTSAC 2 cut(s) 125, 566
TseI GCWGC 3 cut(s) 7, 1056, 1059
Tsp45I GTSAC 2 cut(s) 125, 566
TspDTI ATGAA 6 cut(s) 11, 142, 146, 284, 423, 1228
TspGWI ACGGA 2 cut(s) 551, 1296
TspRI CASTG 4 cut(s) 531, 856, 1123, 1336
VspI ATTAAT 1 cut(s) 794
XapI RAATTY 5 cut(s) 285, 327, 1121, 1170, 1319
XcmI CCANNNNNNNNNTGG 2 cut(s) 78, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.