Rw4G016560

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008769
Physical Location & Seq
Forward (+)
37730212 .. 37734359
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016560.1

Sequence Viewer

Length: 756 bp
ATGGCTGTTCTTAACTGCCTTCACAAACAGCTGCTGATATATAATGATGGCACAAATTTTTCTCTTGTACTTGATAATCTTCCACATGATCTCATCTATTCTGAAAATCAAGTTTCACCATGGAAGGAAGTTTGGGTGGAGAACCAAGATGGAGAAACACTATCTGAATTGTACAAGCCATTGCAAGACTCACTAATTAAGCGATGCATTGAGATCATCGACTTCGAGAAAAAACATGATTCTGAAAGCAGTGCATTATTGAAAGCAAAAAGTGTTTGGTCTAAAGTCTTCCCAAAACCTCGAAGAAGTTGGCTACCAACTGGTTGCTTGAAACTACTTGAAGTGTTACATGGAGCACTACCAAAGATGTCTTTGATTGCTTCAGACTTTAGTTACCTACCTGACGTGAGAATACTTGGTGAAAGAGCTCCATTGGTTTCTACCAAGAAAGATGGCAGCAGCTCAGACTATGGCAGTTATCTTGATGCAAAGGGGGATGCTGATATCTTCTTCCCCACTGAATTTTGGTTATTGGAACGTATTGATCATTACTGTTCTGGATGGATGAAGCTGCAAAAAGATAACTCATCCAAAGAAGGAAAGAAGAGGCGAACAATTATACTTGATACATCTTCATTCATGGAAGAATTTGGATTGCCCTCGAGGACAAGAACCAAGGATGGATACAATCCACTTTTGGATGACTTCAAGAATACAAAATTCTATCTAAGTGTCCCAACACATAATACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

251

Amino Acids

28.78

Weight (kDa)

6.32

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 23 - 188 1.2e-11 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 661
AcsI RAATTY 4 cut(s) 55, 521, 647, 719
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 2 cut(s) 69, 173
AgsI TTSAA 4 cut(s) 262, 331, 341, 709
AjiI CACGTC 1 cut(s) 406
AluBI AGCT 4 cut(s) 31, 428, 462, 571
AluI AGCT 4 cut(s) 31, 428, 462, 571
Alw21I GWGCWC 2 cut(s) 358, 430
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 661
ApeKI GCWGC 4 cut(s) 31, 456, 459, 571
ApoI RAATTY 4 cut(s) 55, 521, 647, 719
AsuHPI GGTGA 2 cut(s) 108, 431
AvaI CYCGRG 1 cut(s) 661
BanII GRGCYC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 2 cut(s) 358, 430
BbvI GCAGC 4 cut(s) 18, 468, 471, 558
BccI CCATC 5 cut(s) 41, 143, 446, 555, 674
BciVI GTATCC 1 cut(s) 677
BclI TGATCA 1 cut(s) 544
BfuI GTATCC 1 cut(s) 677
BisI GCNGC 4 cut(s) 32, 457, 460, 572
BlsI GCNGC 4 cut(s) 33, 458, 461, 573
BmeT110I CYCGRG 1 cut(s) 661
BmgBI CACGTC 1 cut(s) 406
BmsI GCATC 3 cut(s) 194, 475, 487
BpiI GAAGAC 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 119, 675
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 119, 675
BseGI GGATG 6 cut(s) 502, 566, 570, 587, 685, 706
BseMI GCAATG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 325
BseXI GCAGC 4 cut(s) 18, 468, 471, 558
BsiHKAI GWGCWC 2 cut(s) 358, 430
BsiHKCI CYCGRG 1 cut(s) 661
BslFI GGGAC 1 cut(s) 719
BsmFI GGGAC 1 cut(s) 719
BsoBI CYCGRG 1 cut(s) 661
Bsp1286I GDGCHC 2 cut(s) 358, 430
Bsp1407I TGTACA 1 cut(s) 171
Bsp143I GATC 3 cut(s) 88, 213, 544
Bsp19I CCATGG 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 179
BsrGI TGTACA 1 cut(s) 171
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 119, 675
BssMI GATC 3 cut(s) 88, 213, 544
BssT1I CCWWGG 2 cut(s) 119, 675
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 599
BstAUI TGTACA 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 463, 728
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 6 cut(s) 502, 566, 570, 587, 685, 706
BstKTI GATC 3 cut(s) 91, 216, 547
BstMBI GATC 3 cut(s) 88, 213, 544
BstV1I GCAGC 4 cut(s) 18, 468, 471, 558
BstV2I GAAGAC 1 cut(s) 280
BsuI GTATCC 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 119
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 6 cut(s) 502, 566, 570, 587, 685, 706
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 256, 516
CaiI CAGNNNCTG 1 cut(s) 34
Csp6I GTAC 2 cut(s) 68, 172
CviAII CATG 5 cut(s) 86, 120, 236, 350, 640
CviJI RGCY 7 cut(s) 5, 31, 178, 313, 428, 462, 571
CviKI_1 RGCY 7 cut(s) 5, 31, 178, 313, 428, 462, 571
CviQI GTAC 2 cut(s) 68, 172
DdeI CTNAG 2 cut(s) 463, 728
DpnI GATC 3 cut(s) 90, 215, 546
DpnII GATC 3 cut(s) 88, 213, 544
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
Ecl136II GAGCTC 1 cut(s) 428
Eco130I CCWWGG 2 cut(s) 119, 675
Eco24I GRGCYC 1 cut(s) 430
Eco32I GATATC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 661
EcoICRI GAGCTC 1 cut(s) 428
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 2 cut(s) 119, 675
EcoT22I ATGCAT 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 2 cut(s) 119, 675
FaeI CATG 5 cut(s) 89, 123, 239, 353, 643
FaqI GGGAC 1 cut(s) 719
FatI CATG 5 cut(s) 85, 119, 235, 349, 639
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 4 cut(s) 32, 457, 460, 572
FokI GGATG 6 cut(s) 509, 573, 574, 577, 692, 713
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 4 cut(s) 32, 457, 460, 572
GluI GCNGC 4 cut(s) 32, 457, 460, 572
Hin1II CATG 5 cut(s) 89, 123, 239, 353, 643
HinfI GANTC 2 cut(s) 188, 239
HphI GGTGA 2 cut(s) 108, 431
Hpy188I TCNGA 5 cut(s) 103, 166, 244, 385, 466
Hpy188III TCNNGA 4 cut(s) 226, 482, 558, 709
HpyAV CCTTC 3 cut(s) 29, 118, 590
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 2 cut(s) 405, 538
HpyCH4V TGCA 5 cut(s) 184, 207, 254, 488, 574
HpyF3I CTNAG 2 cut(s) 463, 728
HpySE526I ACGT 2 cut(s) 405, 538
Hsp92II CATG 5 cut(s) 89, 123, 239, 353, 643
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 3 cut(s) 88, 213, 544
LmnI GCTCC 2 cut(s) 353, 433
LpnPI CCDG 3 cut(s) 306, 414, 543
Lsp1109I GCAGC 4 cut(s) 18, 468, 471, 558
LweI GCATC 3 cut(s) 194, 475, 487
MaeII ACGT 2 cut(s) 405, 538
MaeIII GTNAC 2 cut(s) 345, 392
MalI GATC 3 cut(s) 90, 215, 546
MboI GATC 3 cut(s) 88, 213, 544
MboII GAAGA 8 cut(s) 71, 280, 315, 499, 502, 616, 624, 656
MhlI GDGCHC 2 cut(s) 358, 430
MluCI AATT 7 cut(s) 55, 167, 195, 521, 615, 647, 719
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 4 cut(s) 309, 600, 657, 670
Mph1103I ATGCAT 1 cut(s) 209
MseI TTAA 2 cut(s) 12, 198
MspA1I CMGCKG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 119
NdeII GATC 3 cut(s) 88, 213, 544
NlaIII CATG 5 cut(s) 89, 123, 239, 353, 643
NsiI ATGCAT 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 661
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 33, 458, 461, 573
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 430
PspXI VCTCGAGB 1 cut(s) 661
PstNI CAGNNNCTG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 2 cut(s) 69, 173
RsaNI GTAC 2 cut(s) 68, 172
SacI GAGCTC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 12, 198
SatI GCNGC 4 cut(s) 32, 457, 460, 572
Sau3AI GATC 3 cut(s) 88, 213, 544
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 2 cut(s) 358, 430
SetI ASST 9 cut(s) 33, 301, 399, 403, 408, 430, 464, 541, 573
SfaNI GCATC 3 cut(s) 194, 475, 487
Sfr274I CTCGAG 1 cut(s) 661
SlaI CTCGAG 1 cut(s) 661
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
Sse9I AATT 7 cut(s) 55, 167, 195, 521, 615, 647, 719
SstI GAGCTC 1 cut(s) 430
StyI CCWWGG 2 cut(s) 119, 675
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 2 cut(s) 408, 541
TaqI TCGA 4 cut(s) 219, 225, 301, 662
TasI AATT 7 cut(s) 55, 167, 195, 521, 615, 647, 719
TatI WGTACW 2 cut(s) 67, 171
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 12, 198
Tru9I TTAA 2 cut(s) 12, 198
TscAI CASTG 2 cut(s) 256, 523
TseI GCWGC 4 cut(s) 31, 456, 459, 571
TspDTI ATGAA 4 cut(s) 581, 624, 628, 741
TspRI CASTG 2 cut(s) 256, 523
XapI RAATTY 4 cut(s) 55, 521, 647, 719
XhoI CTCGAG 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.