Rroxscaffold_5G00359160

Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH ubiquinone oxidoreductase complex (complex I)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008769
Physical Location & Seq
Forward (+)
39241873 .. 39252186
10314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359160.1

Sequence Viewer

Length: 1299 bp
ATGTGTGCAGTGAACCCATGTGAACTCTGGGCAGTTCCATCCCAAGCCCTTTTCTCCACACACATTGTTGGTGAAAAACCAGTTCTTGTTCGTGATTTCATTCATTCTGCACTATATGACCCTCGACATGGTTATTTCGCTCAAAGATCGAGGTCGGTTGGTGTGCTTCAGAAAGCCATTAAGTTCAATCAGATTGAGGGCAGGAAAGCTTATATGAAATACTTGGATAAAATATACAAGGGAAGTGAGATATCATGGTTTACTCCTGTGGAAATTTTTAAGCCTTGGTATGCCCACGGGATAGCTGAAGCCATAATGCGTACTGCTAACCTTTCAGTTCCCCTCAAGATATATGAAATTGGTGGTGGATCAGGAACTTGTGCAAAGGGAATCATGGATTACATATTGTTGAATGCACCTACCAGAGTTTACAACAATATGACTTACGTCTCAGTTGAAATCAGTCCCTCACTGGCTGAGCTACAAAGAGAAACTGTTGGAGAAGTTCGTAGTCACTTATCAAAGTTTCGAGTCGAATGCCGTGATGCTGCTGACAGGAAGGGATGGGGGGATGTGGAGCAAGAGCCATGCTGGGTAATAATGCTCGAGGTACTTGATAATCTTCCACATGATCTCATCTATTCTGAAAATCAAGTTTCACCATGGAAGGAAGTTTGGGTGGAGAACCAAGATGGAGAAACACTATCTGAATTGTACAAGCCATTGCAAGACTCACTAATTAAGCGATGCATTGAGATCATCGACTTCGAGAAAAAACATGATTCTCAAAGCGGTGCATTATTGAAAGCAAAAAGTGTTTGGTCTAAAGTCTTCCCAAAACCTCGAAGAAGTTGGCTACCAACTGGTTGCTTGAAACTACTTGAAGTGTTACATGGAGCACTACCAAAGATGTCTTTGATTGCTTCAGACTTTAGTTACCTACCTGACGTGAGAATACTTGGTGAAAGAGCTCCATTGGTTTCTACCAAGAAAGATGGCAGCAGCTCAGACTATGGCAGTTATCTTGATGCAAAGGGGGATGCTGATATCTTCTTCCCCACTGAATTTTGGTTATTGGAACGTATTGATCATTACTGTTCTGGATGGATGAAGCTGCAAAAAGATAACTCATCCAAAGAAGGAAAGAAGAGGCGAACAATTATACTTGATACATCTTCATTCATGGAAGAATTTGGATTGCCCTCGAGGACAAGAACCAAGGATGGATACAATCCTCTTTTGGATGACTTCAAGAATACAAAATTCTATCTAAGTGTCCCAACACATAATACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_28

Protein Analysis

432

Amino Acids

49.24

Weight (kDa)

7.58

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_28 PF02636 105 - 369 1.6e-30 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1204
AciI CCGC 1 cut(s) 792
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 4 cut(s) 273, 1064, 1190, 1262
AcuI CTGAAG 3 cut(s) 152, 327, 909
AfaI GTAC 3 cut(s) 322, 612, 716
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 7 cut(s) 187, 412, 458, 805, 874, 884, 1252
AjiI CACGTC 1 cut(s) 949
AluBI AGCT 6 cut(s) 209, 305, 481, 971, 1005, 1114
AluI AGCT 6 cut(s) 209, 305, 481, 971, 1005, 1114
Alw21I GWGCWC 2 cut(s) 901, 973
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 2 cut(s) 605, 1204
ApeKI GCWGC 4 cut(s) 548, 999, 1002, 1114
ApoI RAATTY 4 cut(s) 273, 1064, 1190, 1262
AsuHPI GGTGA 3 cut(s) 83, 651, 974
AvaI CYCGRG 2 cut(s) 605, 1204
BanII GRGCYC 1 cut(s) 973
BbsI GAAGAC 1 cut(s) 823
Bbv12I GWGCWC 2 cut(s) 901, 973
BbvI GCAGC 4 cut(s) 535, 1011, 1014, 1101
BccI CCATC 6 cut(s) 46, 558, 686, 989, 1098, 1217
BceAI ACGGC 1 cut(s) 525
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciVI GTATCC 1 cut(s) 1220
BclI TGATCA 1 cut(s) 1087
BcoDI GTCTC 1 cut(s) 454
BfuI GTATCC 1 cut(s) 1220
BisI GCNGC 4 cut(s) 549, 1000, 1003, 1115
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 4 cut(s) 550, 1001, 1004, 1116
BmeT110I CYCGRG 2 cut(s) 605, 1204
BmgBI CACGTC 1 cut(s) 949
BmsI GCATC 4 cut(s) 535, 737, 1018, 1030
BoxI GACNNNNGTC 1 cut(s) 446
BpiI GAAGAC 1 cut(s) 823
Bpu1102I GCTNAGC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 329
BsaJI CCNNGG 4 cut(s) 284, 295, 662, 1218
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 80, 477, 868
Bse3DI GCAATG 1 cut(s) 722
BseDI CCNNGG 4 cut(s) 284, 295, 662, 1218
BseGI GGATG 9 cut(s) 38, 569, 577, 1045, 1109, 1113, 1130, 1228, 1249
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 3 cut(s) 465, 468, 1020
BseNI ACTGG 3 cut(s) 80, 477, 868
BseXI GCAGC 4 cut(s) 535, 1011, 1014, 1101
BseYI CCCAGC 1 cut(s) 591
BsgI GTGCAG 2 cut(s) 27, 93
BsiHKAI GWGCWC 2 cut(s) 901, 973
BsiHKCI CYCGRG 2 cut(s) 605, 1204
BslFI GGGAC 2 cut(s) 450, 1262
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 454
BsmBI CGTCTC 1 cut(s) 454
BsmFI GGGAC 2 cut(s) 450, 1262
BsmI GAATGC 2 cut(s) 418, 542
BsoBI CYCGRG 2 cut(s) 605, 1204
Bsp1286I GDGCHC 2 cut(s) 901, 973
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 5 cut(s) 146, 368, 631, 756, 1087
Bsp1720I GCTNAGC 1 cut(s) 477
Bsp19I CCATGG 1 cut(s) 662
BspACI CCGC 1 cut(s) 792
BspCNI CTCAG 3 cut(s) 464, 469, 1019
BspPI GGATC 1 cut(s) 376
BsrDI GCAATG 1 cut(s) 722
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 80, 477, 868
BssECI CCNNGG 4 cut(s) 284, 295, 662, 1218
BssMI GATC 5 cut(s) 146, 368, 631, 756, 1087
BssT1I CCWWGG 3 cut(s) 284, 662, 1218
Bst4CI ACNGT 2 cut(s) 496, 1097
Bst6I CTCTTC 1 cut(s) 1142
BstAUI TGTACA 1 cut(s) 714
BstDEI CTNAG 4 cut(s) 451, 477, 1006, 1271
BstDSI CCRYGG 2 cut(s) 295, 662
BstF5I GGATG 9 cut(s) 38, 569, 577, 1045, 1109, 1113, 1130, 1228, 1249
BstKTI GATC 5 cut(s) 149, 371, 634, 759, 1090
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 5 cut(s) 146, 368, 631, 756, 1087
BstPAI GACNNNNGTC 1 cut(s) 446
BstV1I GCAGC 4 cut(s) 535, 1011, 1014, 1101
BstV2I GAAGAC 1 cut(s) 823
BsuI GTATCC 1 cut(s) 1220
BtgI CCRYGG 2 cut(s) 295, 662
BtgZI GCGATG 1 cut(s) 760
BtrI CACGTC 1 cut(s) 949
BtsCI GGATG 9 cut(s) 38, 569, 577, 1045, 1109, 1113, 1130, 1228, 1249
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 3 cut(s) 15, 470, 1059
Csp6I GTAC 3 cut(s) 321, 611, 715
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviQI GTAC 3 cut(s) 321, 611, 715
DdeI CTNAG 4 cut(s) 451, 477, 1006, 1271
DpnI GATC 5 cut(s) 148, 370, 633, 758, 1089
DpnII GATC 5 cut(s) 146, 368, 631, 756, 1087
Eam1104I CTCTTC 1 cut(s) 1142
EarI CTCTTC 1 cut(s) 1142
Ecl136II GAGCTC 1 cut(s) 971
Eco130I CCWWGG 3 cut(s) 284, 662, 1218
Eco24I GRGCYC 1 cut(s) 973
Eco32I GATATC 2 cut(s) 252, 1048
Eco53kI GAGCTC 1 cut(s) 971
Eco57I CTGAAG 3 cut(s) 152, 327, 909
Eco88I CYCGRG 2 cut(s) 605, 1204
EcoICRI GAGCTC 1 cut(s) 971
EcoRV GATATC 2 cut(s) 252, 1048
EcoT14I CCWWGG 3 cut(s) 284, 662, 1218
EcoT22I ATGCAT 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 973
ErhI CCWWGG 3 cut(s) 284, 662, 1218
Esp3I CGTCTC 1 cut(s) 454
FaqI GGGAC 2 cut(s) 450, 1262
FbaI TGATCA 1 cut(s) 1087
Fnu4HI GCNGC 4 cut(s) 549, 1000, 1003, 1115
FokI GGATG 9 cut(s) 25, 576, 584, 1052, 1116, 1117, 1120, 1235, 1256
FriOI GRGCYC 1 cut(s) 973
Fsp4HI GCNGC 4 cut(s) 549, 1000, 1003, 1115
GluI GCNGC 4 cut(s) 549, 1000, 1003, 1115
GsaI CCCAGC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 4 cut(s) 390, 531, 731, 782
HphI GGTGA 3 cut(s) 83, 651, 974
Hpy166II GTNNAC 4 cut(s) 13, 23, 261, 430
Hpy188I TCNGA 6 cut(s) 171, 192, 646, 709, 928, 1009
Hpy188III TCNNGA 7 cut(s) 92, 346, 372, 769, 1025, 1101, 1252
Hpy8I GTNNAC 4 cut(s) 13, 23, 261, 430
HpyAV CCTTC 3 cut(s) 553, 661, 1133
HpyCH4III ACNGT 2 cut(s) 496, 1097
HpyCH4IV ACGT 3 cut(s) 447, 948, 1081
HpyCH4V TGCA 9 cut(s) 8, 110, 383, 416, 727, 750, 797, 1031, 1117
HpyF3I CTNAG 4 cut(s) 451, 477, 1006, 1271
HpySE526I ACGT 3 cut(s) 447, 948, 1081
Ksp22I TGATCA 1 cut(s) 1087
Kzo9I GATC 5 cut(s) 146, 368, 631, 756, 1087
LmnI GCTCC 3 cut(s) 577, 896, 976
Lsp1109I GCAGC 4 cut(s) 535, 1011, 1014, 1101
LweI GCATC 4 cut(s) 535, 737, 1018, 1030
MaeII ACGT 3 cut(s) 447, 948, 1081
MaeIII GTNAC 3 cut(s) 512, 888, 935
MalI GATC 5 cut(s) 148, 370, 633, 758, 1089
MboI GATC 5 cut(s) 146, 368, 631, 756, 1087
MboII GAAGA 8 cut(s) 614, 823, 858, 1042, 1045, 1159, 1167, 1199
MhlI GDGCHC 2 cut(s) 901, 973
MluCI AATT 8 cut(s) 273, 357, 710, 738, 1064, 1158, 1190, 1262
MlyI GAGTC 2 cut(s) 540, 725
MmeI TCCRAC 1 cut(s) 478
Mph1103I ATGCAT 1 cut(s) 752
MseI TTAA 3 cut(s) 180, 279, 741
Mva1269I GAATGC 2 cut(s) 418, 542
NcoI CCATGG 1 cut(s) 662
NdeII GATC 5 cut(s) 146, 368, 631, 756, 1087
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 752
PaeR7I CTCGAG 2 cut(s) 605, 1204
PctI GAATGC 2 cut(s) 418, 542
PfeI GAWTC 2 cut(s) 390, 782
PkrI GCNGC 4 cut(s) 550, 1001, 1004, 1116
PleI GAGTC 2 cut(s) 539, 725
PpsI GAGTC 2 cut(s) 539, 725
PshAI GACNNNNGTC 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 591
PspXI VCTCGAGB 2 cut(s) 605, 1204
RsaI GTAC 3 cut(s) 322, 612, 716
RsaNI GTAC 3 cut(s) 321, 611, 715
SacI GAGCTC 1 cut(s) 973
SaqAI TTAA 3 cut(s) 180, 279, 741
SatI GCNGC 4 cut(s) 549, 1000, 1003, 1115
Sau3AI GATC 5 cut(s) 146, 368, 631, 756, 1087
SchI GAGTC 2 cut(s) 540, 725
SduI GDGCHC 2 cut(s) 901, 973
SfaNI GCATC 4 cut(s) 535, 737, 1018, 1030
Sfr274I CTCGAG 2 cut(s) 605, 1204
SlaI CTCGAG 2 cut(s) 605, 1204
SmlI CTYRAG 3 cut(s) 344, 605, 1204
SmoI CTYRAG 3 cut(s) 344, 605, 1204
Sse9I AATT 8 cut(s) 273, 357, 710, 738, 1064, 1158, 1190, 1262
SsiI CCGC 1 cut(s) 792
SstI GAGCTC 1 cut(s) 973
StyI CCWWGG 3 cut(s) 284, 662, 1218
TaaI ACNGT 2 cut(s) 496, 1097
TaiI ACGT 3 cut(s) 450, 951, 1084
TaqI TCGA 9 cut(s) 124, 149, 529, 534, 606, 762, 768, 844, 1205
TasI AATT 8 cut(s) 273, 357, 710, 738, 1064, 1158, 1190, 1262
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 2 cut(s) 390, 782
Tru1I TTAA 3 cut(s) 180, 279, 741
Tru9I TTAA 3 cut(s) 180, 279, 741
TscAI CASTG 3 cut(s) 15, 477, 1066
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 4 cut(s) 548, 999, 1002, 1114
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 8 cut(s) 88, 92, 230, 369, 1124, 1167, 1171, 1284
TspRI CASTG 3 cut(s) 15, 477, 1066
XapI RAATTY 4 cut(s) 273, 1064, 1190, 1262
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XhoI CTCGAG 2 cut(s) 605, 1204
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.