pycom04g00730

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014577
Physical Location & Seq
Forward (+)
698834 .. 701236
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGGCTCCACTCTCACTCTCCAACCAGCACCCATTAATCTCCTTTCTCTTCTTCCTCACACCCGTTGTCATGGTGGTGGGCATGCTTGGAGTTCTGGGGGTGGAGGGAGCAAATTCTCCGACGTCTTCCAGCACAACATGGTGTGTGGCTAGGAGTGATGCGAGCGCACAGGCTCTTCAGACAGCCTTGGACTATGCATGCGCAGCTGGTGCGGACTGTTCTCCAATCCAACCCGACGGACTATGTTATCTCCCAAACACCATCCAAGCTCATGCCTCTTATGCCTTCAACAGTTACTACCAGCGCCAGGCCAAGGCCCCTGGTTCTTGCGGCTTCTCCGGCACTGCCACCATTGCCCAAACAGACCCCAGTTATGGATCTTGTGATTACCCATCTTCCACAAGTAGACGCGGCTGGAGGGACAACTTCACCTTCCACACCACCAACAGTTCTGAATCCACAAACGCCACCTTCGACGTTCGCACCACCCTTCACCAGCGGATTCACTCCCGGATCGACCCCGATCGATCCCCGACGATAACTCAAAGGCGTCCCTCGATTCCATGTCCCCAACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

194

Amino Acids

20.78

Weight (kDa)

6.93

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 46 - 117 1.6e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
Acc16I TGCGCA 1 cut(s) 202
AccI GTMKAC 1 cut(s) 406
AccII CGCG 1 cut(s) 411
AciI CCGC 4 cut(s) 212, 330, 411, 499
AclWI GGATC 3 cut(s) 385, 521, 522
AcsI RAATTY 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 2 cut(s) 122, 550
AfiI CCNNNNNNNGG 3 cut(s) 307, 313, 374
AgsI TTSAA 1 cut(s) 289
AjnI CCWGG 2 cut(s) 306, 319
AluBI AGCT 2 cut(s) 206, 269
AluI AGCT 2 cut(s) 206, 269
AlwI GGATC 3 cut(s) 385, 521, 522
AoxI GGCC 2 cut(s) 309, 315
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 1 cut(s) 112
AseI ATTAAT 1 cut(s) 35
AspLEI GCGC 3 cut(s) 167, 203, 306
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 2 cut(s) 421, 485
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 215
BccI CCATC 2 cut(s) 269, 400
BciT130I CCWGG 2 cut(s) 308, 321
BcnI CCSGG 1 cut(s) 511
BfaI CTAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 3 cut(s) 204, 331, 412
BlsI GCNGC 3 cut(s) 205, 332, 413
Bme1390I CCNGG 3 cut(s) 308, 321, 511
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 6, 318
BmrFI CCNGG 3 cut(s) 308, 321, 511
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 436
BpuMI CCSGG 1 cut(s) 511
Bsa29I ATCGAT 1 cut(s) 526
BsaHI GRCGYC 2 cut(s) 122, 550
BsaJI CCNNGG 3 cut(s) 186, 312, 319
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 313, 374
Bse1I ACTGG 1 cut(s) 369
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 308, 321
BseCI ATCGAT 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 186, 312, 319
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 3 cut(s) 307, 313, 374
BseMI GCAATG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 2 cut(s) 311, 317
BshVI ATCGAT 1 cut(s) 526
BsiEI CGRYCG 1 cut(s) 526
BsiSI CCGG 2 cut(s) 339, 511
BslFI GGGAC 3 cut(s) 434, 537, 552
BslI CCNNNNNNNGG 3 cut(s) 307, 313, 374
BsmFI GGGAC 3 cut(s) 434, 537, 552
BsnI GGCC 2 cut(s) 311, 317
Bsp143I GATC 4 cut(s) 377, 513, 523, 527
BspACI CCGC 4 cut(s) 212, 330, 411, 499
BspANI GGCC 2 cut(s) 311, 317
BspDI ATCGAT 1 cut(s) 526
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 2 cut(s) 6, 318
BspPI GGATC 3 cut(s) 385, 521, 522
BspQI GCTCTTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 1 cut(s) 369
BssECI CCNNGG 3 cut(s) 186, 312, 319
BssMI GATC 4 cut(s) 377, 513, 523, 527
BssNI GRCGYC 2 cut(s) 122, 550
BssT1I CCWWGG 2 cut(s) 186, 312
Bst2UI CCWGG 2 cut(s) 308, 321
Bst4CI ACNGT 3 cut(s) 218, 293, 449
Bst6I CTCTTC 2 cut(s) 53, 180
BstACI GRCGYC 2 cut(s) 122, 550
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 83, 163, 199
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 3 cut(s) 167, 203, 306
BstKTI GATC 4 cut(s) 380, 516, 526, 530
BstMBI GATC 4 cut(s) 377, 513, 523, 527
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 5 cut(s) 203, 209, 281, 339, 353
BstNI CCWGG 2 cut(s) 308, 321
BstNSI RCATGY 2 cut(s) 85, 201
BstSCI CCNGG 3 cut(s) 306, 319, 509
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
Bsu15I ATCGAT 1 cut(s) 526
BsuRI GGCC 2 cut(s) 311, 317
BsuTUI ATCGAT 1 cut(s) 526
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 3 cut(s) 83, 163, 199
CfoI GCGC 3 cut(s) 167, 203, 306
Cfr13I GGNCC 1 cut(s) 316
ClaI ATCGAT 1 cut(s) 526
CseI GACGC 2 cut(s) 417, 539
CviAII CATG 6 cut(s) 70, 82, 138, 198, 272, 564
DpnI GATC 4 cut(s) 379, 515, 525, 529
DpnII GATC 4 cut(s) 377, 513, 523, 527
Eam1104I CTCTTC 2 cut(s) 53, 180
EarI CTCTTC 2 cut(s) 53, 180
Eco130I CCWWGG 2 cut(s) 186, 312
Eco57I CTGAAG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 2 cut(s) 306, 319
EcoT14I CCWWGG 2 cut(s) 186, 312
EcoT22I ATGCAT 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 186, 312
FaeI CATG 6 cut(s) 73, 85, 141, 201, 275, 567
FaqI GGGAC 3 cut(s) 434, 537, 552
FatI CATG 6 cut(s) 69, 81, 137, 197, 271, 563
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 3 cut(s) 204, 331, 412
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 3 cut(s) 204, 331, 412
FspBI CTAG 1 cut(s) 150
FspI TGCGCA 1 cut(s) 202
GlaI GCGC 3 cut(s) 166, 202, 305
GluI GCNGC 3 cut(s) 204, 331, 412
GsuI CTGGAG 1 cut(s) 436
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 2 cut(s) 311, 317
HapII CCGG 2 cut(s) 339, 511
HgaI GACGC 2 cut(s) 417, 539
HhaI GCGC 3 cut(s) 167, 203, 306
Hin1I GRCGYC 2 cut(s) 122, 550
Hin1II CATG 6 cut(s) 73, 85, 141, 201, 275, 567
Hin6I GCGC 3 cut(s) 165, 201, 304
HinP1I GCGC 3 cut(s) 165, 201, 304
HinfI GANTC 3 cut(s) 455, 502, 559
HpaII CCGG 2 cut(s) 339, 511
HphI GGTGA 2 cut(s) 421, 485
Hpy166II GTNNAC 1 cut(s) 407
Hpy188I TCNGA 3 cut(s) 120, 180, 454
Hpy188III TCNNGA 1 cut(s) 579
Hpy8I GTNNAC 1 cut(s) 407
Hpy99I CGWCG 4 cut(s) 124, 239, 479, 538
HpyAV CCTTC 4 cut(s) 295, 442, 481, 500
HpyCH4III ACNGT 3 cut(s) 218, 293, 449
HpyCH4IV ACGT 2 cut(s) 122, 477
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 209, 281, 339, 353
HpySE526I ACGT 2 cut(s) 122, 477
Hsp92I GRCGYC 2 cut(s) 122, 550
Hsp92II CATG 6 cut(s) 73, 85, 141, 201, 275, 567
HspAI GCGC 3 cut(s) 165, 201, 304
Kzo9I GATC 4 cut(s) 377, 513, 523, 527
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 10, 107
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 122, 477
MaeIII GTNAC 1 cut(s) 293
MalI GATC 4 cut(s) 379, 515, 525, 529
MboI GATC 4 cut(s) 377, 513, 523, 527
MboII GAAGA 5 cut(s) 40, 43, 117, 167, 387
MflI RGATCY 1 cut(s) 377
MluCI AATT 1 cut(s) 112
MmeI TCCRAC 3 cut(s) 45, 143, 253
MnlI CCTC 5 cut(s) 65, 97, 286, 411, 565
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 2 cut(s) 206, 499
MspI CCGG 2 cut(s) 339, 511
MspR9I CCNGG 3 cut(s) 308, 321, 511
MvaI CCWGG 2 cut(s) 308, 321
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 5 cut(s) 203, 209, 281, 339, 353
NciI CCSGG 1 cut(s) 511
NdeII GATC 4 cut(s) 377, 513, 523, 527
NlaIII CATG 6 cut(s) 73, 85, 141, 201, 275, 567
NlaIV GGNNCC 2 cut(s) 6, 318
NsbI TGCGCA 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 2 cut(s) 85, 201
PaeI GCATGC 2 cut(s) 85, 201
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PfeI GAWTC 3 cut(s) 455, 502, 559
PfoI TCCNGGA 1 cut(s) 509
PkrI GCNGC 3 cut(s) 205, 332, 413
Ple19I CGATCG 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 306, 319
PspGI CCWGG 2 cut(s) 306, 319
PspN4I GGNNCC 2 cut(s) 6, 318
PspPI GGNCC 1 cut(s) 316
PsuI RGATCY 1 cut(s) 377
PvuI CGATCG 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 206
RseI CAYNNNNRTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 3 cut(s) 204, 331, 412
Sau3AI GATC 4 cut(s) 377, 513, 523, 527
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 3 cut(s) 308, 321, 511
SetI ASST 6 cut(s) 125, 208, 271, 434, 473, 480
SfaNI GCATC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 74
SphI GCATGC 2 cut(s) 85, 201
Sse9I AATT 1 cut(s) 112
SsiI CCGC 4 cut(s) 212, 330, 411, 499
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 3 cut(s) 306, 319, 509
StyI CCWWGG 2 cut(s) 186, 312
TaaI ACNGT 3 cut(s) 218, 293, 449
TaiI ACGT 2 cut(s) 125, 480
TaqI TCGA 4 cut(s) 474, 516, 526, 557
TasI AATT 1 cut(s) 112
TauI GCSGC 2 cut(s) 333, 414
TfiI GAWTC 3 cut(s) 455, 502, 559
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 203
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 349
VspI ATTAAT 1 cut(s) 35
XapI RAATTY 1 cut(s) 112
XceI RCATGY 2 cut(s) 85, 201
XmiI GTMKAC 1 cut(s) 406
XspI CTAG 1 cut(s) 150
ZraI GACGTC 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.