pycom04g04590

Belongs to the bacterial ribosomal protein bL35 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013010
Physical Location & Seq
Reverse (-)
4205911 .. 4207488
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGAGGAGTACAAACTTCATCCATAAGCGTTTGCTGTCAGTGGAAGAAGAGAAAGTAAGAATGGCTTGTGTAGGTGTAACCATGACCATGGCGGCATCGTTTGGTCTGAGATACTACCCATCGTGCTCTTACCCAACTCAACTCAATCACGGCTCAGTTCAACTCGGTAACTTCAAGAGGCCCAAACCCTTGAGACTCAGTTCCTCGCAATCGCATAGCATTTCTGGGTTGGACTCGCTTCTTCCCCACAAGCTCTCCGCTATCAATTTCACCCCTCCTCGCCGCCTTAGCTCTCTTACCATCGTCTCCGCCAAAGGCTACAAAATGAAGACCCACAAGGCTTCGGCGAAGCGCTTCAGGGTGACGGGTAGAGGAAAAATAGTTCGGAGGAGAGCTGGAAAGCAACATTTGCTTGTAAAGAAGAATGCCAAGAGGAGAACTCGACTCTCCAAAATGCATGCAGTTAGTCGAAGTGACTATGACAACGTGATTGGAGCCTTGCCATACCTAAAAGTAAATAGGAAGGCAGAGTAA

Protein Analysis

178

Amino Acids

19.98

Weight (kDa)

11.41

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 107 - 163 1.4e-19 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24090
fragaria_vesca FvH4_4g09690 FvH4_4g09690
malus_domestica MD04G1052200.v1.1
prunus_persica Prupe.1G102100_v2.0.a1
pyrus_communis pycom04g04590
rosa_chinensis RchiOBHm_Chr4g0405501
rosa_laevigata RLG00000008838
rosa_multiflora Rmu_sc0003981.1_g000024 Rmu_sc0005023.1_g000021
rosa_roxburghii Rroxscaffold_5G00350000
rosa_rugosa Rorug04G0060600
rosa_samantha Rh4AG132300 Rh4BG127200 Rh4CG139800 Rh4DG126600
rosa_wichuraiana Rw0G018140 Rw4G010710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 92, 258, 283, 309
AcuI CTGAAG 1 cut(s) 340
AfaI GTAC 1 cut(s) 10
AfeI AGCGCT 1 cut(s) 353
AgsI TTSAA 2 cut(s) 161, 175
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
AluBI AGCT 3 cut(s) 253, 291, 395
AluI AGCT 3 cut(s) 253, 291, 395
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 187, 310
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 262, 373
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 128
BccI CCATC 2 cut(s) 127, 308
BceAI ACGGC 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 187, 310
BfoI RGCGCY 1 cut(s) 355
BisI GCNGC 2 cut(s) 93, 283
BlsI GCNGC 2 cut(s) 94, 284
BmgT120I GGNCC 1 cut(s) 180
BmiI GGNNCC 1 cut(s) 496
BmsI GCATC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 335
BplI GAGNNNNNCTC 2 cut(s) 424, 456
Bpu10I CCTNAGC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 18
BseMII CTCAG 3 cut(s) 98, 168, 211
BseRI GAGGAG 4 cut(s) 19, 267, 403, 448
BshFI GGCC 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 128
BsmAI GTCTC 2 cut(s) 187, 310
BsmBI CGTCTC 1 cut(s) 310
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 128
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 4 cut(s) 92, 258, 283, 309
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 3 cut(s) 99, 167, 210
BspLI GGNNCC 1 cut(s) 496
BssECI CCNNGG 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 4 cut(s) 107, 154, 197, 287
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 18
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 1 cut(s) 354
BstMAI GTCTC 2 cut(s) 187, 310
BstMWI GCNNNNNNNGC 2 cut(s) 288, 409
BstNSI RCATGY 1 cut(s) 461
BstV2I GAAGAC 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuRI GGCC 1 cut(s) 181
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 459
CfoI GCGC 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 9
CviAII CATG 3 cut(s) 82, 88, 458
CviJI RGCY 9 cut(s) 65, 153, 181, 253, 291, 318, 341, 395, 497
CviKI_1 RGCY 9 cut(s) 65, 153, 181, 253, 291, 318, 341, 395, 497
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 4 cut(s) 107, 154, 197, 287
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 87
Eco47III AGCGCT 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 3 cut(s) 85, 91, 461
FaiI YATR 7 cut(s) 24, 83, 89, 216, 459, 480, 505
FalI AAGNNNNNCTT 2 cut(s) 49, 81
FatI CATG 3 cut(s) 81, 87, 457
Fnu4HI GCNGC 2 cut(s) 93, 283
FokI GGATG 1 cut(s) 5
Fsp4HI GCNGC 2 cut(s) 93, 283
GlaI GCGC 1 cut(s) 353
GluI GCNGC 2 cut(s) 93, 283
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 1 cut(s) 181
HhaI GCGC 1 cut(s) 354
Hin1II CATG 3 cut(s) 85, 91, 461
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HinfI GANTC 3 cut(s) 195, 233, 444
HphI GGTGA 2 cut(s) 262, 373
Hpy188I TCNGA 2 cut(s) 108, 387
Hpy188III TCNNGA 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 517
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 2 cut(s) 457, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 409
HpyF3I CTNAG 4 cut(s) 107, 154, 197, 287
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 3 cut(s) 85, 91, 461
HspAI GCGC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 494
LpnPI CCDG 3 cut(s) 210, 343, 381
LweI GCATC 1 cut(s) 104
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 4 cut(s) 76, 167, 361, 473
MboII GAAGA 5 cut(s) 56, 59, 233, 340, 433
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 1 cut(s) 265
MlyI GAGTC 3 cut(s) 189, 227, 438
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 171, 214, 285, 288, 365, 381, 426
Mph1103I ATGCAT 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 353
MslI CAYNNNNRTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 430
MwoI GCNNNNNNNGC 2 cut(s) 288, 409
NcoI CCATGG 1 cut(s) 87
NlaIII CATG 3 cut(s) 85, 91, 461
NlaIV GGNNCC 1 cut(s) 496
NmuCI GTSAC 2 cut(s) 361, 473
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 353
PkrI GCNGC 2 cut(s) 94, 284
PleI GAGTC 3 cut(s) 189, 227, 438
PpsI GAGTC 3 cut(s) 189, 227, 438
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 180
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 86
SatI GCNGC 2 cut(s) 93, 283
Sau96I GGNCC 1 cut(s) 180
SchI GAGTC 3 cut(s) 189, 227, 438
SduI GDGCHC 1 cut(s) 128
SetI ASST 6 cut(s) 76, 255, 293, 397, 489, 510
SfaNI GCATC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
SphI GCATGC 1 cut(s) 461
Sse9I AATT 1 cut(s) 265
SsiI CCGC 4 cut(s) 92, 258, 283, 309
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 442, 469
TasI AATT 1 cut(s) 265
TatI WGTACW 1 cut(s) 8
TauI GCSGC 2 cut(s) 95, 285
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 361, 473
Tsp45I GTSAC 2 cut(s) 361, 473
TspDTI ATGAA 2 cut(s) 7, 341
TspRI CASTG 1 cut(s) 45
XceI RCATGY 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.