Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0010414

Physical Location & Seq

Genomic Coordinates Reverse (-)

5369676 .. 5379030

Gene Structure 9355 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGCACTGCAATTTGGCAGAAATGCGTTCCTTTGATCAAAACTATGAACGACACTTCGCAGAAACTGTGAGCGAGTGTCGTTTTTCACTTCACTCACTGCGCACGCGACACCTGTCCCGAGCAACAGTCGTGTATATGAGTAGCACCCTCGGCCGGAACAGAAGCTGGCGGAGAGAGAGTAGGAAGAAAATGGAAGACGAAGGAGGAGAGAGGTCGAGCATCGTCGTCGGTCTCATCGAGAACAGAGCCAAGGAGGTTGGAGTGGCAGCCTTTGATTTAAGATTAGCTTCTCTGCATCTTTCTCAATATATTGAAACTAGCAGTTCATATCAGAATACGAAAACCTTGCTGCAATTCTATGATCCTATGGTGATTATTGTTTCACCGAACAAGCTGGCACCTGATGGTATGGTTGGAGTTTCAGAGTTGGTGGACAGATTTTATGCTACAGTCAAGAAGGTTGTAATGGCCCGTGGTTGCTTCGACGACACTAAGGGTGCCGTACTGATTAAAAATCTAGCAGCCAAGGAACCTTCAGCACTAGGATTGGATACTTATTACAAGCAGTATTACCTCTGCTTAGCTGCTGCTGCAGCTACAATTAAGTGGATAGAAGCAGAGAAAGGAGTCATTGTCACAAACCACTCACTTGTGGTCACTTTTAATGGATCATTTGCTCATATGAATATTGATGCGACTAGTGTCCAAAACTTGGAAATCATTGAGCCACTTCATTCGACACTTTGGGGCACCAGCAACAAGAAAAGAAGTTTATTAAACATGTTTAAGACAACAAAAACAGTTGGAGGGTATGAGGGGCTGGTTCACTGTATTATTATCCGGGATTTCATGTCCAGACTTCTTCGTGCCAATCTTTTGCAGCCTTTAAAAGATATCGAAACTATAAATGCTCGTCTAGATTGCCTGGATGAGCTCATGAGCAACGAACAGCTGTTCTTTGGACTAGCTCAGGTTCTGCGTAAATTTCCAAAAGAGAGTGATAGGGTACTTTGTCACTTTTGCTTCAAGCCAAAGAAAATTACAAACAAAGTTGTGGGTGTTGATTGTGCTAGAAAAAGCCAAGTGTTGGTTTCGAGTATCATTCTTCTGAAAACTGCTCTAGATGCCTTGCCTTTACTCTCAAAGGTTCTTAAAGATGCAAAGTGTTTTCTTCTTGCAAATGTTTACCAGTCTGTATGTGAAAATGAGAAGTATGCTGCCCTTAGAAAGAGGATTGGAGAGGTTATTGATGAGGATGTGCTTCATGCACGGGTTTCATTTGTAGCCCGCACACAGCAGTGTTTTGCTGTCAAAGCTGGAATCGATGGACTCTTGGATATTGCACGGAGATCATTTTGTGATACTAGTGAAGCTATACATAATCTTGCTAACAAGTATCGTGAAGATTTCAAGTTGCCCAATTTGAAACTCCCGTTTAACAATAGGCAAGGGTTCTACTTTAGCATTCCACATAAGGACATTCAAGGGAAACTTCCTAGCCAGTTCATTCAGGTATTGAAACATGGGAATAATATACACTGCTCAACCTTGGAGCTTGCTTCTCTAAACGTTAGAAATAAGTCTGCAGCTGCAGAATGCTACGTACGTACAGAAGTTTGCCTAGAAGAATTAGTAGATGCCATACGAGAGGATGTTTCTGCACTCACACTGCTGGCAGAGGTCTTATGCCTTTTGGATATGATTGTTAATTCATTTGCACATGCCATTTCCACAAAGCCTGCTGATCGTTATTCTAGACCCGAATTCACAGATAATGGCCCAATGGCAATTGATGCTGGAAGACACCCTATCCTAGAGACTATACACAATGACTTTGTCCCCAACAATATCTTTTTATCAGAGGCATCAAACATGGTGCTCATCATGGGCCCAAACATGAGTGGAAAGAGCACTTATCTTCAACAAGTGTGTCTTATAGTTATTCTTGCTCAGATGGGTTGCTATGTACCAGCTCGCTTTGCAACTCTAAGGGTTGTTGATCGTATATTTACAAGGATGGGTGCAGTAGATAATCTTGAATCGAACTCTAGTACGTTCATGACAGAAATGAAAGAAACAGCTTTTATCATGCAAAATGTCTCCCAGAGGAGTTTGGTTATCATGGATGAACTTGGGAGGGCCACATCTTCCTCTGATGGATTTGCAATTGCTTGGAGCTGCTGCGAACATTTATTATCACTGAAAGCGTACACAATATTTGCTACTCATATGGAGAACCTATCTGAACTAGTAACCATCTACCCAAATGTGAAGATACTTCACTTCGACGTTGATATTAAAAATAATCGTTTAGAGTTCAAGTTTCAACTGAAGGATGGGCCGAGACATGTTCCACACTATGGTCTTTTGTTGGCAGAAGTGGCAGGATTACCAAGCTCCGTGATGGAAACAGCCAGAAACATCACATCCAGGATGACAGAGAAGGAAGTGAAGAGGATGGAAGTAAACTGCCTGCACTACCATCCAGTCCAGATGGCTTACCACGTTGCACAGCGGCTGATATGTTTGAAGTACTCGAGCCAAGATGAGGATTCGATCAGAGAAGCACTACATAATTTGAAAGAGAGCTACATTCATGGAATGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000793 GO:0000794 GO:0000795 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0098813 GO:0099086 GO:0140013 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MutS_III MutS_IV MutS_V

Protein Analysis

870

Amino Acids

98.04

Weight (kDa)

8.37

Isoelectric Point (pI)

44.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_III	PF05192	234 - 569	1.3e-18	MutS domain III
MutS_IV	PF05190	440 - 529	6.6e-12	MutS family domain IV
MutS_V	PF00488	627 - 812	4.1e-69	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010414)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17380 AT4G17380
fragaria_vesca	FvH4_5g19230 FvH4_5g19230
malus_domestica	MD04G1062200.v1.1
prunus_persica	Prupe.5G066100_v2.0.a1 Prupe.5G066100_v2.0.a1
pyrus_communis	pycom04g05580
rosa_chinensis	RchiOBHm_Chr7g0203731
rosa_laevigata	RLG00000003509
rosa_multiflora	Rmu_sc0001714.1_g000006 Rmu_sc0005354.1_g000001
rosa_roxburghii	Rroxscaffold_3G00253590
rosa_rugosa	Rorug07G0078500 Rorug07G0078600 Rorug07G0078700
rosa_samantha	Rh7AG208200 Rh7BG207200 Rh7CG220400 Rh7DG215100
rosa_wichuraiana	Rw7G018130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	101
AccB1I	GGYRCC	3 cut(s)	397, 497, 749
AccB7I	CCANNNNNTGG	3 cut(s)	712, 1087, 2360
AccII	CGCG	1 cut(s)	106
AciI	CCGC	3 cut(s)	169, 1288, 2515
AclI	AACGTT	1 cut(s)	1569
AclWI	GGATC	2 cut(s)	356, 676
AcoI	YGGCCR	1 cut(s)	151
AcsI	RAATTY	2 cut(s)	983, 1763
AcuI	CTGAAG	2 cut(s)	519, 2351
AfaI	GTAC	8 cut(s)	504, 1008, 1605, 1609, 1968, 2053, 2210, 2534
AfiI	CCNNNNNNNGG	5 cut(s)	153, 712, 1087, 2360, 2548
AflIII	ACRYGT	2 cut(s)	780, 2347
AhlI	ACTAGT	3 cut(s)	698, 1364, 2248
AjnI	CCWGG	2 cut(s)	924, 2429
AleI	CACNNNNGTG	1 cut(s)	1297
Alw21I	GWGCWC	3 cut(s)	936, 1881, 1913
Alw26I	GTCTC	4 cut(s)	236, 1811, 2104, 2338
AlwI	GGATC	2 cut(s)	356, 676
AlwNI	CAGNNNCTG	4 cut(s)	65, 165, 976, 2518
Ama87I	CYCGRG	2 cut(s)	117, 2536
AoxI	GGCC	6 cut(s)	151, 468, 1777, 1888, 2139, 2339
ApaI	GGGCCC	1 cut(s)	1892
ApoI	RAATTY	2 cut(s)	983, 1763
AspLEI	GCGC	1 cut(s)	102
AspS9I	GGNCC	6 cut(s)	469, 1778, 1888, 1889, 2139, 2339
AsuC2I	CCSGG	1 cut(s)	842
AsuHPI	GGTGA	2 cut(s)	375, 382
AvaI	CYCGRG	2 cut(s)	117, 2536
BaeGI	GKGCMC	2 cut(s)	752, 1892
BanI	GGYRCC	3 cut(s)	397, 497, 749
BanII	GRGCYC	2 cut(s)	936, 1892
BbsI	GAAGAC	2 cut(s)	201, 1807
Bbv12I	GWGCWC	3 cut(s)	936, 1881, 1913
BceAI	ACGGC	1 cut(s)	485
BcgI	CGANNNNNNTGC	4 cut(s)	208, 242, 328, 362
BciT130I	CCWGG	2 cut(s)	926, 2431
BciVI	GTATCC	1 cut(s)	544
BclI	TGATCA	1 cut(s)	34
BcnI	CCSGG	1 cut(s)	842
BcoDI	GTCTC	4 cut(s)	236, 1811, 2104, 2338
BcuI	ACTAGT	3 cut(s)	698, 1364, 2248
BfmI	CTRYAG	5 cut(s)	447, 591, 1584, 1590, 2606
BfuI	GTATCC	1 cut(s)	544
BlpI	GCTNAGC	1 cut(s)	580
BmcAI	AGTACT	1 cut(s)	2534
Bme1390I	CCNGG	3 cut(s)	842, 926, 2431
BmeT110I	CYCGRG	2 cut(s)	117, 2536
BmgT120I	GGNCC	6 cut(s)	469, 1778, 1888, 1889, 2139, 2339
BmiI	GGNNCC	5 cut(s)	399, 499, 531, 751, 1890
BmrFI	CCNGG	3 cut(s)	842, 926, 2431
BmsI	GCATC	8 cut(s)	228, 304, 682, 1114, 1147, 1627, 1783, 1874
BoxI	GACNNNNGTC	2 cut(s)	112, 701
BpiI	GAAGAC	2 cut(s)	201, 1807
Bpu10I	CCTNAGC	1 cut(s)	969
Bpu1102I	GCTNAGC	1 cut(s)	580
BpuMI	CCSGG	1 cut(s)	842
Bsa29I	ATCGAT	1 cut(s)	1323
BsaAI	YACGTR	2 cut(s)	1603, 1607
BsaI	GGTCTC	1 cut(s)	236
BsaJI	CCNNGG	5 cut(s)	148, 249, 472, 525, 1548
BsaXI	ACNNNNNCTCC	4 cut(s)	163, 193, 618, 648
Bsc4I	CCNNNNNNNGG	5 cut(s)	153, 712, 1087, 2360, 2548
Bse1I	ACTGG	3 cut(s)	1189, 1501, 2486
BseBI	CCWGG	2 cut(s)	926, 2431
BseCI	ATCGAT	1 cut(s)	1323
BseDI	CCNNGG	5 cut(s)	148, 249, 472, 525, 1548
BseLI	CCNNNNNNNGG	5 cut(s)	153, 712, 1087, 2360, 2548
BseMII	CTCAG	2 cut(s)	983, 1964
BseNI	ACTGG	3 cut(s)	1189, 1501, 2486
BseRI	GAGGAG	2 cut(s)	219, 2122
BseSI	GKGCMC	2 cut(s)	752, 1892
BseX3I	CGGCCG	1 cut(s)	151
BsgI	GTGCAG	3 cut(s)	1644, 2043, 2459
Bsh1236I	CGCG	1 cut(s)	106
Bsh1285I	CGRYCG	1 cut(s)	154
BshFI	GGCC	6 cut(s)	153, 470, 1779, 1890, 2141, 2341
BshNI	GGYRCC	3 cut(s)	397, 497, 749
BshVI	ATCGAT	1 cut(s)	1323
BsiEI	CGRYCG	1 cut(s)	154
BsiHKAI	GWGCWC	3 cut(s)	936, 1881, 1913
BsiHKCI	CYCGRG	2 cut(s)	117, 2536
BsiSI	CCGG	2 cut(s)	154, 841
BsiWI	CGTACG	1 cut(s)	1603
BslFI	GGGAC	2 cut(s)	100, 1823
BslI	CCNNNNNNNGG	5 cut(s)	153, 712, 1087, 2360, 2548
BsmAI	GTCTC	4 cut(s)	236, 1811, 2104, 2338
BsmFI	GGGAC	2 cut(s)	100, 1823
BsmI	GAATGC	3 cut(s)	1464, 1601, 2607
BsnI	GGCC	6 cut(s)	153, 470, 1779, 1890, 2141, 2341
Bso31I	GGTCTC	1 cut(s)	236
BsoBI	CYCGRG	2 cut(s)	117, 2536
Bsp120I	GGGCCC	1 cut(s)	1888
Bsp1286I	GDGCHC	5 cut(s)	752, 936, 1881, 1892, 1913
Bsp143I	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
Bsp1720I	GCTNAGC	1 cut(s)	580
BspACI	CCGC	3 cut(s)	169, 1288, 2515
BspANI	GGCC	6 cut(s)	153, 470, 1779, 1890, 2141, 2341
BspCNI	CTCAG	2 cut(s)	982, 1963
BspDI	ATCGAT	1 cut(s)	1323
BspFNI	CGCG	1 cut(s)	106
BspHI	TCATGA	2 cut(s)	936, 2058
BspLI	GGNNCC	5 cut(s)	399, 499, 531, 751, 1890
BspMAI	CTGCAG	3 cut(s)	595, 1588, 1594
BspPI	GGATC	2 cut(s)	356, 676
BspT107I	GGYRCC	3 cut(s)	397, 497, 749
BspTNI	GGTCTC	1 cut(s)	236
BsrI	ACTGG	3 cut(s)	1189, 1501, 2486
BssECI	CCNNGG	5 cut(s)	148, 249, 472, 525, 1548
BssMI	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
BssT1I	CCWWGG	3 cut(s)	249, 525, 1548
Bst2UI	CCWGG	2 cut(s)	926, 2431
Bst4CI	ACNGT	5 cut(s)	67, 127, 451, 802, 830
Bst6I	CTCTTC	1 cut(s)	2447
BstAPI	GCANNNNNTGC	1 cut(s)	1793
BstBAI	YACGTR	2 cut(s)	1603, 1607
BstC8I	GCNNGC	9 cut(s)	104, 167, 396, 1288, 1557, 1674, 1740, 1975, 2474
BstDEI	CTNAG	6 cut(s)	492, 580, 969, 1223, 1950, 1988
BstDSI	CCRYGG	1 cut(s)	472
BstFNI	CGCG	1 cut(s)	106
BstHHI	GCGC	1 cut(s)	102
BstKTI	GATC	7 cut(s)	37, 364, 671, 1352, 1747, 2002, 2559
BstMAI	GTCTC	4 cut(s)	236, 1811, 2104, 2338
BstMBI	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
BstMCI	CGRYCG	1 cut(s)	154
BstMWI	GCNNNNNNNGC	8 cut(s)	150, 590, 593, 1124, 1313, 1793, 1979, 2381
BstNI	CCWGG	2 cut(s)	926, 2431
BstNSI	RCATGY	3 cut(s)	784, 1724, 2351
BstPAI	GACNNNNGTC	2 cut(s)	112, 701
BstSCI	CCNGG	3 cut(s)	840, 924, 2429
BstSFI	CTRYAG	5 cut(s)	447, 591, 1584, 1590, 2606
BstSLI	GKGCMC	2 cut(s)	752, 1892
BstSNI	TACGTA	2 cut(s)	1603, 1607
BstUI	CGCG	1 cut(s)	106
BstV2I	GAAGAC	2 cut(s)	201, 1807
BstZI	CGGCCG	1 cut(s)	151
Bsu15I	ATCGAT	1 cut(s)	1323
BsuI	GTATCC	1 cut(s)	544
BsuRI	GGCC	6 cut(s)	153, 470, 1779, 1890, 2141, 2341
BsuTUI	ATCGAT	1 cut(s)	1323
BtgI	CCRYGG	1 cut(s)	472
BtsI	GCAGTG	5 cut(s)	4, 95, 1304, 1537, 1667
BtsIMutI	CAGTG	7 cut(s)	4, 95, 826, 1304, 1537, 1667, 2198
Cac8I	GCNNGC	9 cut(s)	104, 167, 396, 1288, 1557, 1674, 1740, 1975, 2474
CaiI	CAGNNNCTG	4 cut(s)	65, 165, 976, 2518
CciI	TCATGA	2 cut(s)	936, 2058
CfoI	GCGC	1 cut(s)	102
Cfr13I	GGNCC	6 cut(s)	469, 1778, 1888, 1889, 2139, 2339
ClaI	ATCGAT	1 cut(s)	1323
Csp6I	GTAC	8 cut(s)	503, 1007, 1604, 1608, 1967, 2052, 2209, 2533
CviQI	GTAC	8 cut(s)	503, 1007, 1604, 1608, 1967, 2052, 2209, 2533
DdeI	CTNAG	6 cut(s)	492, 580, 969, 1223, 1950, 1988
DpnI	GATC	7 cut(s)	36, 363, 670, 1351, 1746, 2001, 2558
DpnII	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
DraI	TTTAAA	1 cut(s)	888
EaeI	YGGCCR	1 cut(s)	151
EagI	CGGCCG	1 cut(s)	151
Eam1104I	CTCTTC	1 cut(s)	2447
EarI	CTCTTC	1 cut(s)	2447
EciI	GGCGGA	1 cut(s)	184
Ecl136II	GAGCTC	1 cut(s)	934
EclXI	CGGCCG	1 cut(s)	151
Eco105I	TACGTA	2 cut(s)	1603, 1607
Eco130I	CCWWGG	3 cut(s)	249, 525, 1548
Eco24I	GRGCYC	2 cut(s)	936, 1892
Eco31I	GGTCTC	1 cut(s)	236
Eco32I	GATATC	1 cut(s)	895
Eco52I	CGGCCG	1 cut(s)	151
Eco53kI	GAGCTC	1 cut(s)	934
Eco57I	CTGAAG	2 cut(s)	519, 2351
Eco88I	CYCGRG	2 cut(s)	117, 2536
EcoICRI	GAGCTC	1 cut(s)	934
EcoRI	GAATTC	1 cut(s)	1763
EcoRII	CCWGG	2 cut(s)	924, 2429
EcoRV	GATATC	1 cut(s)	895
EcoT14I	CCWWGG	3 cut(s)	249, 525, 1548
EcoT38I	GRGCYC	2 cut(s)	936, 1892
ErhI	CCWWGG	3 cut(s)	249, 525, 1548
FalI	AAGNNNNNCTT	6 cut(s)	271, 303, 1476, 1508, 1917, 1949
FaqI	GGGAC	2 cut(s)	100, 1823
FauI	CCCGC	1 cut(s)	1295
FauNDI	CATATG	2 cut(s)	681, 2229
FbaI	TGATCA	1 cut(s)	34
FriOI	GRGCYC	2 cut(s)	936, 1892
FspI	TGCGCA	1 cut(s)	101
GlaI	GCGC	1 cut(s)	101
HaeIII	GGCC	6 cut(s)	153, 470, 1779, 1890, 2141, 2341
HapII	CCGG	2 cut(s)	154, 841
HhaI	GCGC	1 cut(s)	102
Hin6I	GCGC	1 cut(s)	100
HinP1I	GCGC	1 cut(s)	100
HinfI	GANTC	5 cut(s)	627, 1320, 1329, 2039, 2552
HpaII	CCGG	2 cut(s)	154, 841
HphI	GGTGA	2 cut(s)	375, 382
Hpy166II	GTNNAC	5 cut(s)	433, 826, 1186, 2211, 2467
Hpy188I	TCNGA	8 cut(s)	333, 424, 1110, 1861, 1953, 2155, 2245, 2561
Hpy8I	GTNNAC	5 cut(s)	433, 826, 1186, 2211, 2467
Hpy99I	CGWCG	4 cut(s)	227, 230, 488, 2291
HpyAV	CCTTC	5 cut(s)	194, 451, 543, 2326, 2437
HpyCH4III	ACNGT	5 cut(s)	67, 127, 451, 802, 830
HpyCH4IV	ACGT	6 cut(s)	1569, 1602, 1606, 2054, 2289, 2505
HpyF10VI	GCNNNNNNNGC	8 cut(s)	150, 590, 593, 1124, 1313, 1793, 1979, 2381
HpyF3I	CTNAG	6 cut(s)	492, 580, 969, 1223, 1950, 1988
HpySE526I	ACGT	6 cut(s)	1569, 1602, 1606, 2054, 2289, 2505
HspAI	GCGC	1 cut(s)	100
Ksp22I	TGATCA	1 cut(s)	34
Kzo9I	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
LmnI	GCTCC	3 cut(s)	1552, 2175, 2402
LweI	GCATC	8 cut(s)	228, 304, 682, 1114, 1147, 1627, 1783, 1874
MaeII	ACGT	6 cut(s)	1569, 1602, 1606, 2054, 2289, 2505
MaeIII	GTNAC	4 cut(s)	634, 655, 1013, 2251
MalI	GATC	7 cut(s)	36, 363, 670, 1351, 1746, 2001, 2558
MboI	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
MfeI	CAATTG	2 cut(s)	1788, 2166
MhlI	GDGCHC	5 cut(s)	752, 936, 1881, 1892, 1913
MlyI	GAGTC	2 cut(s)	636, 1323
MmeI	TCCRAC	3 cut(s)	238, 394, 784
MslI	CAYNNNNRTG	1 cut(s)	1297
MspA1I	CMGCKG	3 cut(s)	952, 1589, 2515
MspI	CCGG	2 cut(s)	154, 841
MspR9I	CCNGG	3 cut(s)	842, 926, 2431
MunI	CAATTG	2 cut(s)	1788, 2166
Mva1269I	GAATGC	3 cut(s)	1464, 1601, 2607
MvaI	CCWGG	2 cut(s)	926, 2431
MvnI	CGCG	1 cut(s)	106
MwoI	GCNNNNNNNGC	8 cut(s)	150, 590, 593, 1124, 1313, 1793, 1979, 2381
NciI	CCSGG	1 cut(s)	842
NdeI	CATATG	2 cut(s)	681, 2229
NdeII	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
NlaIV	GGNNCC	5 cut(s)	399, 499, 531, 751, 1890
NmeAIII	GCCGAG	2 cut(s)	129, 2367
NmuCI	GTSAC	3 cut(s)	634, 655, 1013
NsbI	TGCGCA	1 cut(s)	101
NspI	RCATGY	3 cut(s)	784, 1724, 2351
OliI	CACNNNNGTG	1 cut(s)	1297
PaeR7I	CTCGAG	1 cut(s)	2536
PagI	TCATGA	2 cut(s)	936, 2058
PciI	ACATGT	2 cut(s)	780, 2347
PcsI	WCGNNNNNNNCGW	1 cut(s)	234
PctI	GAATGC	3 cut(s)	1464, 1601, 2607
PfeI	GAWTC	3 cut(s)	1320, 2039, 2552
Pfl23II	CGTACG	1 cut(s)	1603
PflFI	GACNNNGTC	1 cut(s)	1835
PflMI	CCANNNNNTGG	3 cut(s)	712, 1087, 2360
PfoI	TCCNGGA	2 cut(s)	840, 2429
PleI	GAGTC	2 cut(s)	635, 1323
PpsI	GAGTC	2 cut(s)	635, 1323
Ppu21I	YACGTR	2 cut(s)	1603, 1607
PscI	ACATGT	2 cut(s)	780, 2347
PshAI	GACNNNNGTC	2 cut(s)	112, 701
Psp124BI	GAGCTC	1 cut(s)	936
Psp1406I	AACGTT	1 cut(s)	1569
Psp6I	CCWGG	2 cut(s)	924, 2429
PspGI	CCWGG	2 cut(s)	924, 2429
PspLI	CGTACG	1 cut(s)	1603
PspN4I	GGNNCC	5 cut(s)	399, 499, 531, 751, 1890
PspOMI	GGGCCC	1 cut(s)	1888
PspPI	GGNCC	6 cut(s)	469, 1778, 1888, 1889, 2139, 2339
PspXI	VCTCGAGB	1 cut(s)	2536
PstI	CTGCAG	3 cut(s)	595, 1588, 1594
PstNI	CAGNNNCTG	4 cut(s)	65, 165, 976, 2518
PsyI	GACNNNGTC	1 cut(s)	1835
PvuII	CAGCTG	2 cut(s)	952, 1589
RsaI	GTAC	8 cut(s)	504, 1008, 1605, 1609, 1968, 2053, 2210, 2534
RsaNI	GTAC	8 cut(s)	503, 1007, 1604, 1608, 1967, 2052, 2209, 2533
RseI	CAYNNNNRTG	1 cut(s)	1297
SacI	GAGCTC	1 cut(s)	936
Sau3AI	GATC	7 cut(s)	34, 361, 668, 1349, 1744, 1999, 2556
Sau96I	GGNCC	6 cut(s)	469, 1778, 1888, 1889, 2139, 2339
ScaI	AGTACT	1 cut(s)	2534
SchI	GAGTC	2 cut(s)	636, 1323
ScrFI	CCNGG	3 cut(s)	842, 926, 2431
SduI	GDGCHC	5 cut(s)	752, 936, 1881, 1892, 1913
SfaNI	GCATC	8 cut(s)	228, 304, 682, 1114, 1147, 1627, 1783, 1874
SfcI	CTRYAG	5 cut(s)	447, 591, 1584, 1590, 2606
Sfr274I	CTCGAG	1 cut(s)	2536
SlaI	CTCGAG	1 cut(s)	2536
SmiMI	CAYNNNNRTG	1 cut(s)	1297
SmlI	CTYRAG	1 cut(s)	2536
SmoI	CTYRAG	1 cut(s)	2536
SnaBI	TACGTA	2 cut(s)	1603, 1607
SpeI	ACTAGT	3 cut(s)	698, 1364, 2248
SsiI	CCGC	3 cut(s)	169, 1288, 2515
SspI	AATATT	2 cut(s)	688, 2217
SstI	GAGCTC	1 cut(s)	936
StyD4I	CCNGG	3 cut(s)	840, 924, 2429
StyI	CCWWGG	3 cut(s)	249, 525, 1548
TaaI	ACNGT	5 cut(s)	67, 127, 451, 802, 830
TaiI	ACGT	6 cut(s)	1572, 1605, 1609, 2057, 2292, 2508
TaqII	GACCGA	1 cut(s)	218
TatI	WGTACW	1 cut(s)	2532
TauI	GCSGC	1 cut(s)	2518
TfiI	GAWTC	3 cut(s)	1320, 2039, 2552
TscAI	CASTG	6 cut(s)	102, 833, 1304, 1544, 1674, 2205
TseFI	GTSAC	3 cut(s)	634, 655, 1013
Tsp45I	GTSAC	3 cut(s)	634, 655, 1013
TspGWI	ACGGA	2 cut(s)	1360, 2389
TspRI	CASTG	6 cut(s)	102, 833, 1304, 1544, 1674, 2205
Tth111I	GACNNNGTC	1 cut(s)	1835
Van91I	CCANNNNNTGG	3 cut(s)	712, 1087, 2360
XapI	RAATTY	2 cut(s)	983, 1763
XbaI	TCTAGA	3 cut(s)	916, 1120, 1754
XceI	RCATGY	3 cut(s)	784, 1724, 2351
XhoI	CTCGAG	1 cut(s)	2536
ZrmI	AGTACT	1 cut(s)	2534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.