pycom04g09560

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002650
Physical Location & Seq
Forward (+)
12278150 .. 12280719
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1641 bp
ATGGGGCATAGACATCAGTTCAGCACATCTCATGTTTTTGAAAGTGAAAATGATCAGAATTGGAATCATATGAATACGGAAGAACCTTTTGGGCATTTAGGCCAGGCTGGCTCTGGGGAGAACGGTTCTTTCTTTTATCCTGTAGAAAATATGTTTATAGATGGAGCGCCTTTTGCTTCAAATTGGAACCCGGCTCCGAGGTCATATGGGTACTCTTCCGTGAACCACAATATTGATGTACCACATTATCAACCTGATGCTACTGGTCCATCTCATGACCCTTTTCAGCATCCAATAAGTGCTGGTACCTTTGGCATGTCCAGTGGGAATTATGCTCACCATGCACCTTCTTCTAGTTATGACCGGCGTACATTCCATGGTGTTGAGGGTAGTTTTGTTGATCTTACTGTGAGCAACGGAAGAGGTCCTCACAAGAGAAAAAGCCCAGGGATCCCTTCAGTATCTGAGAGAGGCAGCACCAGCAGATACTATAGTGCTGGAAGTAGTTCCTCTGAGCTCCCTAGATCTTCAGAGTTGCAGCAGGAGAAAGTAAACATGGATACCTCACACGTGCCTTGGGATCACATTTCTATGGGCCCCAGCTATAGAGGGAGTGGACTCTCAATTAGGGGTGAAGGATCCATGAGAAATGTGAGAAGCCGGTCTGAACATGATTTGGAGTCCAACCTAGCGAGGACACATTTATCGACCAATCCTTTGCACACTTCTTATTCTACAAATCTCCCTATTGACCATTCTAGTACAATGGATCTCTCTGGTCAGGGTTCCACTGGTTTGACCAATGAGTGGAACCATATTAGTGTGGCTCCTCATGGAAGGATTATAGCTCCAGATTCAAGTGGTTTCGGTCACGATCCGAACCACTTCCTTGTGGGTAGTGGTAATGCTTCTACGGATACTGGGTTATACCATCATGATTTCATGTTGAGCAGAAGTAATGCGGTCCCTCAAAGTTATCCTGGTGCCTTAGCCCCAACTGTAAGGGGTGTTCGCAGTACCTACTCCCAAAGATCTACCCCAGCCTTCAGGGCTTCTTCAAGCAACTTGCGATTGGGACATGTAGCACATTCAGATGAAGGATTGCAGCTGATGCCAGAAAATTACCCAAGACATCCTAGGCCAGTGGCCTCTGTTGGGTGGCGTCATAATGACAGGAGTGGTAGATTGAGGATGTCTAATGATAGATACCGAGTCCCTGAAGCGTCTGCTCTCCAGGAGCGGTTTACTTCTGAGGGTTTTATGACTGTGGATCGTCCAGCCTTTTATGGGTCCAGAAATATGCTTGATCATCATAGAGATATGAGGCTGGACATAGACAACATGAGTTATGAGGAACTCCTCGCACTTGGCGAAACAATTGGGAATGTGAGCACAGGCTTGTCTGAAGATCTGATACCGAAGTGTTTGACAGAAACAATATATTGTTCATCAGATCAAATCCAGGAGGAAGTCTCATGTGCAATTTGCCTGGAAGAGTACAATGACAGGGATGATGTTGGTGCGCTGAAAAGTTGCGGTCACGATTACCATGTGAGCTGCATCAAGAAGTGGTTGTCAATGAAAAACTCTTGTCCAATCTGCAAAGGTTGTGTTCTTCCTGATAAGATGAAGGAGAAATAA

Protein Analysis

547

Amino Acids

60.39

Weight (kDa)

6.2

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 488 - 535 1.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 492 - 535 3.7e-13 Ring finger domain
zf-C3HC4 PF00097 493 - 534 1.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 493 - 534 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 493 - 532 1.8e-06 RING-type zinc-finger
zf-RING_11 PF17123 493 - 521 7.5e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 2 cut(s) 305, 983
AccB7I CCANNNNNTGG 1 cut(s) 807
AccBSI CCGCTC 1 cut(s) 1240
AciI CCGC 3 cut(s) 962, 1240, 1536
AclWI GGATC 8 cut(s) 445, 458, 588, 633, 646, 777, 869, 1278
AcuI CTGAAG 5 cut(s) 441, 513, 1030, 1239, 1425
AcvI CACGTG 1 cut(s) 571
AcyI GRCGYC 1 cut(s) 1162
AfaI GTAC 7 cut(s) 212, 240, 307, 370, 763, 1018, 1499
AfiI CCNNNNNNNGG 3 cut(s) 807, 1155, 1287
AflIII ACRYGT 2 cut(s) 568, 1078
AgsI TTSAA 4 cut(s) 41, 180, 858, 1059
AjnI CCWGG 6 cut(s) 102, 445, 979, 1233, 1461, 1488
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 5 cut(s) 517, 603, 848, 1108, 1557
AluI AGCT 5 cut(s) 517, 603, 848, 1108, 1557
Alw21I GWGCWC 2 cut(s) 519, 1394
Alw26I GTCTC 1 cut(s) 1477
AlwI GGATC 8 cut(s) 445, 458, 588, 633, 646, 777, 869, 1278
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 4 cut(s) 100, 595, 1139, 1146
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 4 cut(s) 474, 538, 1105, 1557
ArsI GACNNNNNNTTYG 2 cut(s) 700, 732
Asp700I GAANNNNTTC 2 cut(s) 505, 884
Asp718I GGTACC 1 cut(s) 305
AspA2I CCTAGG 1 cut(s) 1136
AspLEI GCGC 2 cut(s) 169, 1525
AspS9I GGNCC 6 cut(s) 266, 425, 595, 596, 964, 1290
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 329, 644
AvaII GGWCC 4 cut(s) 266, 425, 964, 1290
AvrII CCTAGG 1 cut(s) 1136
BaeGI GKGCMC 1 cut(s) 599
BaeI ACNNNNGTAYC 4 cut(s) 478, 511, 1406, 1439
BamHI GGATCC 2 cut(s) 450, 638
BanI GGYRCC 2 cut(s) 305, 983
BanII GRGCYC 2 cut(s) 519, 599
BarI GAAGNNNNNNTAC 2 cut(s) 1398, 1430
BbrPI CACGTG 1 cut(s) 571
Bbv12I GWGCWC 2 cut(s) 519, 1394
BbvI GCAGC 4 cut(s) 486, 550, 1117, 1544
BccI CCATC 3 cut(s) 155, 277, 939
BciT130I CCWGG 6 cut(s) 104, 447, 981, 1235, 1463, 1490
BciVI GTATCC 2 cut(s) 553, 910
BclI TGATCA 2 cut(s) 52, 1306
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 1477
BfaI CTAG 5 cut(s) 354, 522, 689, 759, 1137
BfmI CTRYAG 3 cut(s) 141, 490, 604
BfoI RGCGCY 1 cut(s) 170
BfuI GTATCC 2 cut(s) 553, 910
BglI GCCNNNNNGGC 2 cut(s) 108, 1049
BglII AGATCT 3 cut(s) 524, 1031, 1408
BisI GCNGC 4 cut(s) 475, 539, 1106, 1558
BlnI CCTAGG 1 cut(s) 1136
BlsI GCNGC 4 cut(s) 476, 540, 1107, 1559
Bme1390I CCNGG 7 cut(s) 104, 191, 447, 981, 1235, 1463, 1490
Bme18I GGWCC 4 cut(s) 266, 425, 964, 1290
BmgT120I GGNCC 6 cut(s) 266, 425, 595, 596, 964, 1290
BmrFI CCNGG 7 cut(s) 104, 191, 447, 981, 1235, 1463, 1490
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 4 cut(s) 247, 298, 1101, 1569
BmuI ACTGGG 1 cut(s) 930
BplI GAGNNNNNCTC 2 cut(s) 1457, 1489
BpmI CTGGAG 2 cut(s) 834, 1217
Bpu10I CCTNAGC 1 cut(s) 988
BpuMI CCSGG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 1162
BsaJI CCNNGG 6 cut(s) 197, 376, 445, 446, 575, 1136
Bsc4I CCNNNNNNNGG 3 cut(s) 807, 1155, 1287
Bse118I RCCGGY 2 cut(s) 363, 660
Bse1I ACTGG 5 cut(s) 268, 321, 796, 925, 1142
BseBI CCWGG 6 cut(s) 104, 447, 981, 1235, 1463, 1490
BseDI CCNNGG 6 cut(s) 197, 376, 445, 446, 575, 1136
BseGI GGATG 4 cut(s) 289, 1132, 1197, 1516
BseLI CCNNNNNNNGG 3 cut(s) 807, 1155, 1287
BseMII CTCAG 3 cut(s) 456, 504, 1242
BseNI ACTGG 5 cut(s) 268, 321, 796, 925, 1142
BseRI GAGGAG 2 cut(s) 819, 1349
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 4 cut(s) 486, 550, 1117, 1544
BseYI CCCAGC 2 cut(s) 599, 1039
BshFI GGCC 4 cut(s) 102, 597, 1141, 1148
BshNI GGYRCC 2 cut(s) 305, 983
BsiHKAI GWGCWC 2 cut(s) 519, 1394
BsiSI CCGG 3 cut(s) 191, 364, 661
BslFI GGGAC 3 cut(s) 950, 1089, 1199
BslI CCNNNNNNNGG 3 cut(s) 807, 1155, 1287
BsmAI GTCTC 1 cut(s) 1477
BsmFI GGGAC 3 cut(s) 950, 1089, 1199
BsnI GGCC 4 cut(s) 102, 597, 1141, 1148
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 3 cut(s) 519, 599, 1394
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 3 cut(s) 962, 1240, 1536
BspANI GGCC 4 cut(s) 102, 597, 1141, 1148
BspCNI CTCAG 3 cut(s) 457, 505, 1243
BspHI TCATGA 2 cut(s) 274, 934
BspPI GGATC 8 cut(s) 445, 458, 588, 633, 646, 777, 869, 1278
BspT107I GGYRCC 2 cut(s) 305, 983
BsrBI CCGCTC 1 cut(s) 1240
BsrFI RCCGGY 2 cut(s) 363, 660
BsrI ACTGG 5 cut(s) 268, 321, 796, 925, 1142
BssAI RCCGGY 2 cut(s) 363, 660
BssECI CCNNGG 6 cut(s) 197, 376, 445, 446, 575, 1136
BssNI GRCGYC 1 cut(s) 1162
BssT1I CCWWGG 3 cut(s) 376, 575, 1136
Bst2UI CCWGG 6 cut(s) 104, 447, 981, 1235, 1463, 1490
Bst4CI ACNGT 4 cut(s) 125, 409, 1000, 1267
Bst6I CTCTTC 3 cut(s) 220, 415, 1488
BstACI GRCGYC 1 cut(s) 1162
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 465, 513, 988, 1251
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 4 cut(s) 289, 1132, 1197, 1516
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 169, 1525
BstMAI GTCTC 1 cut(s) 1477
BstMWI GCNNNNNNNGC 6 cut(s) 108, 173, 341, 480, 1049, 1111
BstNI CCWGG 6 cut(s) 104, 447, 981, 1235, 1463, 1490
BstNSI RCATGY 2 cut(s) 319, 1082
BstSCI CCNGG 7 cut(s) 102, 189, 445, 979, 1233, 1461, 1488
BstSFI CTRYAG 3 cut(s) 141, 490, 604
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 4 cut(s) 486, 550, 1117, 1544
BstX2I RGATCY 6 cut(s) 450, 524, 638, 769, 1031, 1408
BstYI RGATCY 6 cut(s) 450, 524, 638, 769, 1031, 1408
BsuI GTATCC 2 cut(s) 553, 910
BsuRI GGCC 4 cut(s) 102, 597, 1141, 1148
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 4 cut(s) 289, 1132, 1197, 1516
BtsIMutI CAGTG 3 cut(s) 328, 789, 1149
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 464
CciI TCATGA 2 cut(s) 274, 934
CfoI GCGC 2 cut(s) 169, 1525
Cfr10I RCCGGY 2 cut(s) 363, 660
Cfr13I GGNCC 6 cut(s) 266, 425, 595, 596, 964, 1290
CseI GACGC 2 cut(s) 1151, 1212
Csp6I GTAC 7 cut(s) 211, 239, 306, 369, 762, 1017, 1498
CviQI GTAC 7 cut(s) 211, 239, 306, 369, 762, 1017, 1498
DdeI CTNAG 4 cut(s) 465, 513, 988, 1251
Eam1104I CTCTTC 3 cut(s) 220, 415, 1488
EarI CTCTTC 3 cut(s) 220, 415, 1488
Ecl136II GAGCTC 1 cut(s) 517
Eco130I CCWWGG 3 cut(s) 376, 575, 1136
Eco24I GRGCYC 2 cut(s) 519, 599
Eco47I GGWCC 4 cut(s) 266, 425, 964, 1290
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 5 cut(s) 441, 513, 1030, 1239, 1425
Eco72I CACGTG 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 517
EcoO109I RGGNCCY 2 cut(s) 425, 596
EcoRII CCWGG 6 cut(s) 102, 445, 979, 1233, 1461, 1488
EcoT14I CCWWGG 3 cut(s) 376, 575, 1136
EcoT38I GRGCYC 2 cut(s) 519, 599
ErhI CCWWGG 3 cut(s) 376, 575, 1136
FaqI GGGAC 3 cut(s) 950, 1089, 1199
FauNDI CATATG 2 cut(s) 69, 205
FbaI TGATCA 2 cut(s) 52, 1306
Fnu4HI GCNGC 4 cut(s) 475, 539, 1106, 1558
FokI GGATG 4 cut(s) 276, 1119, 1204, 1523
FriOI GRGCYC 2 cut(s) 519, 599
Fsp4HI GCNGC 4 cut(s) 475, 539, 1106, 1558
FspBI CTAG 5 cut(s) 354, 522, 689, 759, 1137
GlaI GCGC 2 cut(s) 168, 1524
GluI GCNGC 4 cut(s) 475, 539, 1106, 1558
GsaI CCCAGC 2 cut(s) 603, 1043
GsuI CTGGAG 2 cut(s) 834, 1217
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 4 cut(s) 102, 597, 1141, 1148
HapII CCGG 3 cut(s) 191, 364, 661
HgaI GACGC 2 cut(s) 1151, 1212
HhaI GCGC 2 cut(s) 169, 1525
Hin1I GRCGYC 1 cut(s) 1162
Hin6I GCGC 2 cut(s) 167, 1523
HinP1I GCGC 2 cut(s) 167, 1523
HinfI GANTC 5 cut(s) 64, 618, 680, 854, 1212
HpaII CCGG 3 cut(s) 191, 364, 661
HphI GGTGA 2 cut(s) 329, 644
Hpy166II GTNNAC 4 cut(s) 223, 553, 617, 1245
Hpy188III TCNNGA 8 cut(s) 275, 851, 872, 935, 1293, 1541, 1564, 1619
Hpy8I GTNNAC 4 cut(s) 223, 553, 617, 1245
HpyAV CCTTC 7 cut(s) 357, 465, 629, 831, 1054, 1091, 1624
HpyCH4III ACNGT 4 cut(s) 125, 409, 1000, 1267
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 7 cut(s) 344, 538, 721, 1105, 1481, 1560, 1602
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 173, 341, 480, 1049, 1111
HpyF3I CTNAG 4 cut(s) 465, 513, 988, 1251
HpySE526I ACGT 1 cut(s) 570
Hsp92I GRCGYC 1 cut(s) 1162
HspAI GCGC 2 cut(s) 167, 1523
KpnI GGTACC 1 cut(s) 309
Ksp22I TGATCA 2 cut(s) 52, 1306
LmnI GCTCC 6 cut(s) 164, 199, 522, 832, 853, 1237
Lsp1109I GCAGC 4 cut(s) 486, 550, 1117, 1544
LweI GCATC 4 cut(s) 247, 298, 1101, 1569
MaeI CTAG 5 cut(s) 354, 522, 689, 759, 1137
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 869, 1538
MbiI CCGCTC 1 cut(s) 1240
MboII GAAGA 9 cut(s) 92, 207, 342, 432, 519, 1047, 1418, 1505, 1607
MfeI CAATTG 1 cut(s) 1377
MflI RGATCY 6 cut(s) 450, 524, 638, 769, 1031, 1408
MhlI GDGCHC 3 cut(s) 519, 599, 1394
MluCI AATT 7 cut(s) 58, 181, 328, 624, 1120, 1377, 1482
MlyI GAGTC 3 cut(s) 612, 689, 1221
MmeI TCCRAC 1 cut(s) 708
MroXI GAANNNNTTC 2 cut(s) 505, 884
MslI CAYNNNNRTG 3 cut(s) 590, 819, 1092
MspA1I CMGCKG 1 cut(s) 1108
MspI CCGG 3 cut(s) 191, 364, 661
MspR9I CCNGG 7 cut(s) 104, 191, 447, 981, 1235, 1463, 1490
MunI CAATTG 1 cut(s) 1377
MvaI CCWGG 6 cut(s) 104, 447, 981, 1235, 1463, 1490
MwoI GCNNNNNNNGC 6 cut(s) 108, 173, 341, 480, 1049, 1111
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 376
NdeI CATATG 2 cut(s) 69, 205
NmuCI GTSAC 2 cut(s) 869, 1538
NspI RCATGY 2 cut(s) 319, 1082
PagI TCATGA 2 cut(s) 274, 934
PasI CCCWGGG 1 cut(s) 446
PciI ACATGT 1 cut(s) 1078
PcsI WCGNNNNNNNCGW 1 cut(s) 1368
PdmI GAANNNNTTC 2 cut(s) 505, 884
PfeI GAWTC 2 cut(s) 64, 854
PflMI CCANNNNNTGG 1 cut(s) 807
PfoI TCCNGGA 2 cut(s) 1233, 1461
PkrI GCNGC 4 cut(s) 476, 540, 1107, 1559
PleI GAGTC 3 cut(s) 612, 688, 1220
PmaCI CACGTG 1 cut(s) 571
PmlI CACGTG 1 cut(s) 571
PpsI GAGTC 3 cut(s) 612, 688, 1220
Ppu21I YACGTR 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 425
PscI ACATGT 1 cut(s) 1078
Psp124BI GAGCTC 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 6 cut(s) 102, 445, 979, 1233, 1461, 1488
PspCI CACGTG 1 cut(s) 571
PspFI CCCAGC 2 cut(s) 599, 1039
PspGI CCWGG 6 cut(s) 102, 445, 979, 1233, 1461, 1488
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 6 cut(s) 266, 425, 595, 596, 964, 1290
PspPPI RGGWCCY 1 cut(s) 425
PsrI GAACNNNNNNTAC 2 cut(s) 993, 1025
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 6 cut(s) 450, 524, 638, 769, 1031, 1408
PvuII CAGCTG 1 cut(s) 1108
RsaI GTAC 7 cut(s) 212, 240, 307, 370, 763, 1018, 1499
RsaNI GTAC 7 cut(s) 211, 239, 306, 369, 762, 1017, 1498
RseI CAYNNNNRTG 3 cut(s) 590, 819, 1092
SacI GAGCTC 1 cut(s) 519
SatI GCNGC 4 cut(s) 475, 539, 1106, 1558
Sau96I GGNCC 6 cut(s) 266, 425, 595, 596, 964, 1290
SchI GAGTC 3 cut(s) 612, 689, 1221
ScrFI CCNGG 7 cut(s) 104, 191, 447, 981, 1235, 1463, 1490
SduI GDGCHC 3 cut(s) 519, 599, 1394
SfaNI GCATC 4 cut(s) 247, 298, 1101, 1569
SfcI CTRYAG 3 cut(s) 141, 490, 604
SinI GGWCC 4 cut(s) 266, 425, 964, 1290
SmiMI CAYNNNNRTG 3 cut(s) 590, 819, 1092
Sse9I AATT 7 cut(s) 58, 181, 328, 624, 1120, 1377, 1482
SsiI CCGC 3 cut(s) 962, 1240, 1536
SspI AATATT 1 cut(s) 232
SspMI CTAG 5 cut(s) 354, 522, 689, 759, 1137
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 7 cut(s) 102, 189, 445, 979, 1233, 1461, 1488
StyI CCWWGG 3 cut(s) 376, 575, 1136
TaaI ACNGT 4 cut(s) 125, 409, 1000, 1267
TaiI ACGT 1 cut(s) 573
TaqI TCGA 1 cut(s) 707
TaqII GACCGA 1 cut(s) 857
TasI AATT 7 cut(s) 58, 181, 328, 624, 1120, 1377, 1482
TatI WGTACW 2 cut(s) 761, 1497
TfiI GAWTC 2 cut(s) 64, 854
TscAI CASTG 3 cut(s) 328, 796, 1149
TseFI GTSAC 2 cut(s) 869, 1538
TseI GCWGC 4 cut(s) 474, 538, 1105, 1557
Tsp45I GTSAC 2 cut(s) 869, 1538
TspDTI ATGAA 5 cut(s) 86, 931, 1110, 1437, 1595
TspGWI ACGGA 4 cut(s) 92, 208, 432, 929
TspRI CASTG 3 cut(s) 328, 796, 1149
Van91I CCANNNNNTGG 1 cut(s) 807
VpaK11BI GGWCC 4 cut(s) 266, 425, 964, 1290
XceI RCATGY 2 cut(s) 319, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmaJI CCTAGG 1 cut(s) 1136
XmnI GAANNNNTTC 2 cut(s) 505, 884
XspI CTAG 5 cut(s) 354, 522, 689, 759, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.