pycom04g10760

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009024
Physical Location & Seq
Forward (+)
13485452 .. 13488080
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGGGCAACTGCCACGGCCACCACCGTAAACGCCGTAACGGCCCCGTCTCCGGTGAAACGGCCAGTTCTGGCTTTAAACATAGTCCGTCGGGTCAAGCAGCTCAAATCCCTTGCTCTAGCTCATCGTCAGCTAAAAAGACCAGCAGCATGGTGGTCGCCTTGCCGTACGCTCACGTCGACTCGAGCTTGCGCGCCCTCGCCGCCCAGGCCGAGGGCTTCGGCCGGCTCGCCGTCGGCGGTCTCAACGGGGCCGTTTACCACGTCACTACTCTAGCAGATGATGGTCCGGGTTCACTTAGGGAAGGATGCCGAAAGAAAGAGCCGCTTTGGATTGTCTTTGAAATTTCAGGCACTATCCATCTCAAGTCTCACTTGAACGTATCATCGTACAAGACTATAGATGGGCGAGGTCAGAGGATTAAACTTACGGGGAAGGGGTTGAGGTTGAAGGAATGTGAACATGTGATTGTATGCAATTTAGAGTTTGAAGGTGGTAGAGGATCAGATGTTGATGGCATCCAAATGAAACCCAATTCTAAACACATATGGATAGATCAGTGCAGCCTTCGTGACTATGAAGACGGACTAATTGATATCTCCAGAGGAAGCACGGATATTACAATTTCGAGGTGTCATTTTTCACAGCATGATAAGACAATACTGATTGGAGCAGATCCCTCGCATATTGATGATAGATGCATCCGGGTTACCATTCACCACTGTTTCTTTGATGGGACCAGACAGAGACATCCTCGTGTTAGATTTGCGAAAGTACATTTATATAACAATTATACCCGAAACTGGGGCATATATGCTGTTTGTGCGAGTGTAGAATCGCAGATATTCTCTGAATGCAATATATATGAAGCAGGAAAGAAAAAAGTGGCATTTAAGTACCTTACGGAGAAGGCAGCGGACAAGGAGACGGCAGTGACTGGCCACATTAGGTCCGAAGGGGACTTATTCGTGACCGGAACTCATGCAGGATTATCACCCGATGGCGGTGAACATTGCATGTTTCATCCTACCCAACACTATCAGACATGGACAAAAGGACCTCCAGCAGATGATCTTAAGCATGTTCTTCAACATTGCACAGGATGGCAGGGTGTTCCGCGGCCAGCTGATCAAGGAGTAGCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

382

Amino Acids

41.86

Weight (kDa)

8.71

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 122 - 317 1.5e-29 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AccII CGCG 2 cut(s) 192, 1117
AciI CCGC 7 cut(s) 201, 237, 323, 914, 1002, 1115, 1117
AclWI GGATC 2 cut(s) 508, 668
AcoI YGGCCR 5 cut(s) 16, 60, 220, 937, 1118
AcsI RAATTY 1 cut(s) 342
AfaI GTAC 4 cut(s) 167, 389, 774, 896
AfiI CCNNNNNNNGG 5 cut(s) 50, 211, 801, 802, 1001
AflII CTTAAG 1 cut(s) 1073
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 5 cut(s) 341, 376, 448, 488, 1088
AjiI CACGTC 2 cut(s) 175, 262
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 6 cut(s) 101, 120, 131, 186, 1124, 1139
AluI AGCT 6 cut(s) 101, 120, 131, 186, 1124, 1139
Alw26I GTCTC 5 cut(s) 52, 245, 372, 739, 917
AlwI GGATC 2 cut(s) 508, 668
AlwNI CAGNNNCTG 1 cut(s) 935
Ama87I CYCGRG 1 cut(s) 181
AoxI GGCC 8 cut(s) 16, 40, 60, 207, 220, 249, 937, 1118
ApeKI GCWGC 4 cut(s) 98, 144, 561, 911
ApoI RAATTY 1 cut(s) 342
AspLEI GCGC 2 cut(s) 192, 194
AspS9I GGNCC 6 cut(s) 41, 249, 284, 735, 948, 1055
AsuC2I CCSGG 2 cut(s) 288, 704
AsuHPI GGTGA 4 cut(s) 65, 707, 984, 1016
AvaI CYCGRG 1 cut(s) 181
AvaII GGWCC 4 cut(s) 284, 735, 948, 1055
BalI TGGCCA 1 cut(s) 939
BauI CACGAG 1 cut(s) 753
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 4 cut(s) 110, 156, 573, 923
BccI CCATC 7 cut(s) 275, 366, 395, 506, 725, 992, 1095
BceAI ACGGC 8 cut(s) 18, 31, 55, 75, 148, 215, 236, 942
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 660, 694
BciT130I CCWGG 1 cut(s) 206
BclI TGATCA 1 cut(s) 1126
BcnI CCSGG 2 cut(s) 288, 704
BcoDI GTCTC 5 cut(s) 52, 245, 372, 739, 917
BfaI CTAG 2 cut(s) 117, 272
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 1073
BglI GCCNNNNNGGC 2 cut(s) 39, 206
BisI GCNGC 7 cut(s) 99, 145, 201, 323, 562, 912, 1118
BlsI GCNGC 7 cut(s) 100, 146, 202, 324, 563, 913, 1119
Bme1390I CCNGG 3 cut(s) 206, 288, 704
Bme18I GGWCC 4 cut(s) 284, 735, 948, 1055
BmeT110I CYCGRG 1 cut(s) 181
BmgBI CACGTC 2 cut(s) 175, 262
BmgT120I GGNCC 6 cut(s) 41, 249, 284, 735, 948, 1055
BmiI GGNNCC 3 cut(s) 43, 250, 736
BmrFI CCNGG 3 cut(s) 206, 288, 704
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 4 cut(s) 296, 525, 686, 708
BmuI ACTGGG 1 cut(s) 811
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 736, 768
BpmI CTGGAG 2 cut(s) 583, 1044
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 2 cut(s) 288, 704
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 4 cut(s) 13, 204, 210, 1115
BsaWI WCCGGW 2 cut(s) 50, 971
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 211, 801, 802, 1001
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 3 cut(s) 63, 806, 940
Bse3DI GCAATG 2 cut(s) 1009, 1090
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 4 cut(s) 13, 204, 210, 1115
BseGI GGATG 6 cut(s) 311, 516, 699, 748, 1021, 1106
BseLI CCNNNNNNNGG 5 cut(s) 50, 211, 801, 802, 1001
BseMI GCAATG 2 cut(s) 1009, 1090
BseNI ACTGG 3 cut(s) 63, 806, 940
BsePI GCGCGC 1 cut(s) 190
BseX3I CGGCCG 1 cut(s) 220
BseXI GCAGC 4 cut(s) 110, 156, 573, 923
BsgI GTGCAG 1 cut(s) 580
Bsh1236I CGCG 2 cut(s) 192, 1117
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 8 cut(s) 18, 42, 62, 209, 222, 251, 939, 1120
BsiEI CGRYCG 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 181
BsiSI CCGG 5 cut(s) 51, 223, 287, 703, 972
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 748, 971
BslI CCNNNNNNNGG 5 cut(s) 50, 211, 801, 802, 1001
BsmAI GTCTC 5 cut(s) 52, 245, 372, 739, 917
BsmBI CGTCTC 2 cut(s) 52, 917
BsmFI GGGAC 2 cut(s) 748, 971
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 8 cut(s) 18, 42, 62, 209, 222, 251, 939, 1120
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 181
Bsp143I GATC 5 cut(s) 500, 553, 673, 1069, 1126
BspACI CCGC 7 cut(s) 201, 237, 323, 914, 1002, 1115, 1117
BspANI GGCC 8 cut(s) 18, 42, 62, 209, 222, 251, 939, 1120
BspFNI CGCG 2 cut(s) 192, 1117
BspLI GGNNCC 3 cut(s) 43, 250, 736
BspPI GGATC 2 cut(s) 508, 668
BspTI CTTAAG 1 cut(s) 1073
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 1009, 1090
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 3 cut(s) 63, 806, 940
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 13, 204, 210, 1115
BssHII GCGCGC 1 cut(s) 190
BssMI GATC 5 cut(s) 500, 553, 673, 1069, 1126
BssSI CACGAG 1 cut(s) 753
Bst2BI CACGAG 1 cut(s) 753
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 26, 722
BstAFI CTTAAG 1 cut(s) 1073
BstC8I GCNNGC 5 cut(s) 188, 192, 224, 228, 1122
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 2 cut(s) 13, 1115
BstEII GGTNACC 1 cut(s) 706
BstF5I GGATG 6 cut(s) 311, 516, 699, 748, 1021, 1106
BstFNI CGCG 2 cut(s) 192, 1117
BstHHI GCGC 2 cut(s) 192, 194
BstKTI GATC 5 cut(s) 503, 556, 676, 1072, 1129
BstMAI GTCTC 5 cut(s) 52, 245, 372, 739, 917
BstMBI GATC 5 cut(s) 500, 553, 673, 1069, 1126
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 4 cut(s) 39, 200, 206, 821
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 3 cut(s) 464, 1018, 1082
BstPI GGTNACC 1 cut(s) 706
BstSCI CCNGG 3 cut(s) 204, 286, 702
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 2 cut(s) 192, 1117
BstV1I GCAGC 4 cut(s) 110, 156, 573, 923
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 1 cut(s) 673
BstZI CGGCCG 1 cut(s) 220
BsuRI GGCC 8 cut(s) 18, 42, 62, 209, 222, 251, 939, 1120
BtgI CCRYGG 2 cut(s) 13, 1115
BtrI CACGTC 2 cut(s) 175, 262
BtsCI GGATG 6 cut(s) 311, 516, 699, 748, 1021, 1106
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 3 cut(s) 563, 718, 936
Cac8I GCNNGC 5 cut(s) 188, 192, 224, 228, 1122
CaiI CAGNNNCTG 1 cut(s) 935
CfoI GCGC 2 cut(s) 192, 194
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 6 cut(s) 41, 249, 284, 735, 948, 1055
Cfr42I CCGCGG 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 166, 388, 773, 895
CviAII CATG 7 cut(s) 148, 461, 647, 980, 1015, 1044, 1079
CviQI GTAC 4 cut(s) 166, 388, 773, 895
DdeI CTNAG 1 cut(s) 296
DpnI GATC 5 cut(s) 502, 555, 675, 1071, 1128
DpnII GATC 5 cut(s) 500, 553, 673, 1069, 1126
DraI TTTAAA 1 cut(s) 76
EaeI YGGCCR 5 cut(s) 16, 60, 220, 937, 1118
EagI CGGCCG 1 cut(s) 220
EclXI CGGCCG 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 4 cut(s) 284, 735, 948, 1055
Eco52I CGGCCG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 181
Eco91I GGTNACC 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 1055
EcoO65I GGTNACC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 595
EcoT22I ATGCAT 1 cut(s) 701
Esp3I CGTCTC 2 cut(s) 52, 917
FaeI CATG 7 cut(s) 151, 464, 650, 983, 1018, 1047, 1082
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 356, 388
FaqI GGGAC 2 cut(s) 748, 971
FatI CATG 7 cut(s) 147, 460, 646, 979, 1014, 1043, 1078
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 1 cut(s) 1126
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 7 cut(s) 99, 145, 201, 323, 562, 912, 1118
FokI GGATG 6 cut(s) 318, 503, 686, 735, 1008, 1113
Fsp4HI GCNGC 7 cut(s) 99, 145, 201, 323, 562, 912, 1118
FspBI CTAG 2 cut(s) 117, 272
GlaI GCGC 2 cut(s) 191, 193
GluI GCNGC 7 cut(s) 99, 145, 201, 323, 562, 912, 1118
GsuI CTGGAG 2 cut(s) 583, 1044
HaeIII GGCC 8 cut(s) 18, 42, 62, 209, 222, 251, 939, 1120
HapII CCGG 5 cut(s) 51, 223, 287, 703, 972
HhaI GCGC 2 cut(s) 192, 194
Hin1II CATG 7 cut(s) 151, 464, 650, 983, 1018, 1047, 1082
Hin6I GCGC 2 cut(s) 190, 192
HinP1I GCGC 2 cut(s) 190, 192
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 179, 833
HpaII CCGG 5 cut(s) 51, 223, 287, 703, 972
HphI GGTGA 4 cut(s) 65, 707, 984, 1016
Hpy166II GTNNAC 6 cut(s) 29, 178, 256, 293, 458, 1007
Hpy188I TCNGA 5 cut(s) 414, 505, 850, 952, 1041
Hpy188III TCNNGA 3 cut(s) 569, 600, 967
Hpy8I GTNNAC 6 cut(s) 29, 178, 256, 293, 458, 1007
Hpy99I CGWCG 3 cut(s) 91, 179, 236
HpyAV CCTTC 7 cut(s) 296, 427, 442, 482, 575, 901, 947
HpyCH4III ACNGT 2 cut(s) 26, 722
HpyCH4IV ACGT 3 cut(s) 174, 261, 378
HpyCH4V TGCA 7 cut(s) 474, 561, 699, 855, 983, 1014, 1095
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 200, 206, 821
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 3 cut(s) 174, 261, 378
Hsp92II CATG 7 cut(s) 151, 464, 650, 983, 1018, 1047, 1082
HspAI GCGC 2 cut(s) 190, 192
KroI GCCGGC 1 cut(s) 222
KroNI GCCGGC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 1126
KspI CCGCGG 1 cut(s) 1118
Kzo9I GATC 5 cut(s) 500, 553, 673, 1069, 1126
LmnI GCTCC 1 cut(s) 668
Lsp1109I GCAGC 4 cut(s) 110, 156, 573, 923
LweI GCATC 4 cut(s) 296, 525, 686, 708
MaeI CTAG 2 cut(s) 117, 272
MaeII ACGT 3 cut(s) 174, 261, 378
MaeIII GTNAC 6 cut(s) 35, 262, 569, 706, 931, 967
MalI GATC 5 cut(s) 502, 555, 675, 1071, 1128
MboI GATC 5 cut(s) 500, 553, 673, 1069, 1126
MboII GAAGA 2 cut(s) 590, 1076
MflI RGATCY 1 cut(s) 673
MlsI TGGCCA 1 cut(s) 939
MluCI AATT 6 cut(s) 342, 475, 532, 588, 621, 787
MluNI TGGCCA 1 cut(s) 939
MlyI GAGTC 1 cut(s) 173
Mox20I TGGCCA 1 cut(s) 939
Mph1103I ATGCAT 1 cut(s) 701
MroNI GCCGGC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 939
MseI TTAA 4 cut(s) 75, 420, 891, 1074
MslI CAYNNNNRTG 3 cut(s) 521, 687, 753
Msp20I TGGCCA 1 cut(s) 939
MspA1I CMGCKG 3 cut(s) 914, 1117, 1124
MspCI CTTAAG 1 cut(s) 1073
MspI CCGG 5 cut(s) 51, 223, 287, 703, 972
MspR9I CCNGG 3 cut(s) 206, 288, 704
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 2 cut(s) 192, 1117
MwoI GCNNNNNNNGC 4 cut(s) 39, 200, 206, 821
NaeI GCCGGC 1 cut(s) 224
NciI CCSGG 2 cut(s) 288, 704
NdeI CATATG 1 cut(s) 545
NdeII GATC 5 cut(s) 500, 553, 673, 1069, 1126
NgoMIV GCCGGC 1 cut(s) 222
NlaIII CATG 7 cut(s) 151, 464, 650, 983, 1018, 1047, 1082
NlaIV GGNNCC 3 cut(s) 43, 250, 736
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 4 cut(s) 262, 569, 931, 967
NsiI ATGCAT 1 cut(s) 701
NspI RCATGY 3 cut(s) 464, 1018, 1082
PaeR7I CTCGAG 1 cut(s) 181
PauI GCGCGC 1 cut(s) 190
PciI ACATGT 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 857
PdiI GCCGGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 833
Pfl23II CGTACG 1 cut(s) 165
PkrI GCNGC 7 cut(s) 100, 146, 202, 324, 563, 913, 1119
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PpuMI RGGWCCY 1 cut(s) 1055
PscI ACATGT 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 204
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 43, 250, 736
PspPI GGNCC 6 cut(s) 41, 249, 284, 735, 948, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PspXI VCTCGAGB 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 935
PsuI RGATCY 1 cut(s) 673
PteI GCGCGC 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 4 cut(s) 167, 389, 774, 896
RsaNI GTAC 4 cut(s) 166, 388, 773, 895
RseI CAYNNNNRTG 3 cut(s) 521, 687, 753
SacII CCGCGG 1 cut(s) 1118
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 4 cut(s) 75, 420, 891, 1074
SatI GCNGC 7 cut(s) 99, 145, 201, 323, 562, 912, 1118
Sau3AI GATC 5 cut(s) 500, 553, 673, 1069, 1126
Sau96I GGNCC 6 cut(s) 41, 249, 284, 735, 948, 1055
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 206, 288, 704
SfaNI GCATC 4 cut(s) 296, 525, 686, 708
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 1118
SgrBI CCGCGG 1 cut(s) 1118
SinI GGWCC 4 cut(s) 284, 735, 948, 1055
SlaI CTCGAG 1 cut(s) 181
SmiMI CAYNNNNRTG 3 cut(s) 521, 687, 753
SmlI CTYRAG 3 cut(s) 181, 362, 1073
SmoI CTYRAG 3 cut(s) 181, 362, 1073
Sse9I AATT 6 cut(s) 342, 475, 532, 588, 621, 787
SsiI CCGC 7 cut(s) 201, 237, 323, 914, 1002, 1115, 1117
SspMI CTAG 2 cut(s) 117, 272
StyD4I CCNGG 3 cut(s) 204, 286, 702
TaaI ACNGT 2 cut(s) 26, 722
TaiI ACGT 3 cut(s) 177, 264, 381
TaqI TCGA 3 cut(s) 177, 182, 626
TasI AATT 6 cut(s) 342, 475, 532, 588, 621, 787
TatI WGTACW 1 cut(s) 772
TauI GCSGC 3 cut(s) 203, 325, 1120
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 4 cut(s) 75, 420, 891, 1074
Tru9I TTAA 4 cut(s) 75, 420, 891, 1074
TscAI CASTG 3 cut(s) 563, 725, 936
TseFI GTSAC 4 cut(s) 262, 569, 931, 967
TseI GCWGC 4 cut(s) 98, 144, 561, 911
Tsp45I GTSAC 4 cut(s) 262, 569, 931, 967
TspDTI ATGAA 4 cut(s) 539, 591, 879, 1010
TspGWI ACGGA 4 cut(s) 75, 597, 626, 917
TspRI CASTG 3 cut(s) 563, 725, 936
Vha464I CTTAAG 1 cut(s) 1073
VpaK11BI GGWCC 4 cut(s) 284, 735, 948, 1055
XapI RAATTY 1 cut(s) 342
XceI RCATGY 3 cut(s) 464, 1018, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 2 cut(s) 117, 272
Zsp2I ATGCAT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.